Anti-TP53BP2 / ASPP2 Antibody (ARG41879)

Product datasheet [email protected]

ARG41879 Package: 100 μl anti-TP53BP2 / ASPP2 antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes TP53BP2 / ASPP2

Tested Reactivity Hu

Tested Application WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name TP53BP2 / ASPP2

Antigen Species Human

Immunogen Recombinant fusion protein corresponding to aa. 200-300 of Human TP53BP2 / ASPP2. (NP_001026855.2)

Conjugation Un-conjugated

Alternate Names P53BP2; Renal carcinoma antigen NY-REN-51; p53-binding protein 2; ASPP2; Tumor suppressor p53-binding protein 2; Apoptosis-stimulating of p53 protein 2; p53BP2; 53BP2; Bcl2-binding protein; PPP1R13A; Bbp; BBP

Application Instructions

Application table Application Dilution

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control DU145

Calculated Mw 126 kDa

Observed Size ~ 170 kDa

Properties

Form Liquid

Purification Affinity purified.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol TP53BP2

Gene Full Name tumor protein p53 binding protein 2

Background This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein- protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis- stimulating activity is inhibited by its interaction with DDX42. [UniProt]

Cellular Localization Cytoplasm, perinuclear region. Nucleus. Note=Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42. [UniProt]

Images

ARG41879 anti-TP53BP2 / ASPP2 antibody WB image

Western blot: 25 µg of DU145 cell lysate stained with ARG41879 anti- TP53BP2 / ASPP2 antibody at 1:1000 dilution.