Interviews of Citology in Uzbek Language Problems and Objectives
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Vocabulario De Morfoloxía, Anatomía E Citoloxía Veterinaria
Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) Servizo de Normalización Lingüística Universidade de Santiago de Compostela COLECCIÓN VOCABULARIOS TEMÁTICOS N.º 4 SERVIZO DE NORMALIZACIÓN LINGÜÍSTICA Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) 2008 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA VOCABULARIO de morfoloxía, anatomía e citoloxía veterinaria : (galego-español- inglés) / coordinador Xusto A. Rodríguez Río, Servizo de Normalización Lingüística ; autores Matilde Lombardero Fernández ... [et al.]. – Santiago de Compostela : Universidade de Santiago de Compostela, Servizo de Publicacións e Intercambio Científico, 2008. – 369 p. ; 21 cm. – (Vocabularios temáticos ; 4). - D.L. C 2458-2008. – ISBN 978-84-9887-018-3 1.Medicina �������������������������������������������������������������������������veterinaria-Diccionarios�������������������������������������������������. 2.Galego (Lingua)-Glosarios, vocabularios, etc. políglotas. I.Lombardero Fernández, Matilde. II.Rodríguez Rio, Xusto A. coord. III. Universidade de Santiago de Compostela. Servizo de Normalización Lingüística, coord. IV.Universidade de Santiago de Compostela. Servizo de Publicacións e Intercambio Científico, ed. V.Serie. 591.4(038)=699=60=20 Coordinador Xusto A. Rodríguez Río (Área de Terminoloxía. Servizo de Normalización Lingüística. Universidade de Santiago de Compostela) Autoras/res Matilde Lombardero Fernández (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal. -
Improvement of Stem Cell-Derived Exosome Release E Ciency By
Improvement of stem cell-derived exosome release eciency by surface modied nanoparticles Dong Jun Park Yonsei University Wonju College of Medicine Wan Su Yun Yonsei University - Wonju Campus Woo Cheol Kim Yonsei University - Wonju Campus Jeong-Eun Park Yonsei University Wonju College of Medicine Su Hoon Lee Yonsei University Wonju College of Medicine Sunmok Ha Yonsei University College of Health Sciences Jin Sil Choi Yonsei University Wonju College of Medicine Jaehong Key Yonsei University - Wonju Campus YoungJoon Seo ( [email protected] ) Yonsei University - Wonju Campus https://orcid.org/0000-0002-2839-4676 Research Keywords: Autophagy, Mesenchymal stem cell-derived exosome, Positively charged nanoparticle, PLGA- PEI NPs, Rab7 Posted Date: November 5th, 2020 DOI: https://doi.org/10.21203/rs.3.rs-42143/v3 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Version of Record: A version of this preprint was published on December 7th, 2020. See the published version at https://doi.org/10.1186/s12951-020-00739-7. Page 1/28 Abstract Background: Mesenchymal stem cells (MSCs) are pluripotent stromal cells that release extracellular vesicles (EVs). EVs contain various growth factors and antioxidants that can positively affect the surrounding cells. Nanoscale MSC-derived EVs, such as exosomes, have been developed as bio-stable nano-type materials. However, some issues, such as low yield and diculty in quantication, limit their use. We hypothesized that enhancing exosome production using nanoparticles would stimulate the release of intracellular molecules. Results: The aim of this study was to elucidate the molecular mechanisms of exosome generation by comparing the internalization of surface-modied, positively charged nanoparticles and exosome generation from MSCs. -
Switch from Translation Initiation to Elongation Needs Not4 and Not5 Collaboration
bioRxiv preprint doi: https://doi.org/10.1101/850859; this version posted November 22, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Switch from translation initiation to elongation needs Not4 and Not5 collaboration George E Allen1°, Olesya O Panasenko1°, Zoltan Villanyi1,&, Marina Zagatti1, Benjamin Weiss1, 2 2 1* 5 Christine Polte , Zoya Ignatova , Martine A Collart 1Department of Microbiology and Molecular Medicine, Institute of Genetics and Genomics Geneva, Faculty of Medicine, University of Geneva, Switzerland 2Biochemistry and Molecular Biology, University of Hamburg, Germany °Equally contributing first authors 10 *Correspondence to: [email protected] &Current Address: Dept of Biochemistry and Molecular Biology, University of Szeged Abstract: Not4 and Not5 are crucial components of the Ccr4-Not complex with pivotal functions in mRNA metabolism. Both associate with ribosomes but mechanistic insights on their 15 function remain elusive. Here we determine that Not5 and Not4 synchronously impact translation initiation and Not5 alone alters translation elongation. Deletion of Not5 causes elongation defects in a codon-dependent fashion, increasing and decreasing the ribosome dwelling occupancy at minor and major codons, respectively. This larger difference in codons’ translation velocities alters translation globally and enables kinetically unfavorable processes 20 such as nascent chain deubiquitination to take place. In turn, this leads to abortive translation and favors protein aggregation. These findings highlight the global impact of Not4 and Not5 in controlling the speed of mRNA translation and transition from initiation to elongation. Summary: Not4 and Not5 regulate translation synchronously but distinguishably, facilitating 25 smooth transition from initiation to elongation Results and discussion The Ccr4-Not complex is a global regulator of mRNA metabolism in eukaryotic cells (for review see (1)). -
Phagocytosis Dynamics Depends on Target Shape
Biophysical Journal Volume 105 September 2013 1143–1150 1143 Phagocytosis Dynamics Depends on Target Shape Debjani Paul,†‡6* Sarra Achouri,†6 Young-Zoon Yoon,† Jurgen Herre,§ Clare E. Bryant,{ and Pietro Cicuta† † ‡ Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom; Department of Biosciences and Bioengineering, § { Indian Institute of Technology Bombay, Powai, Mumbai, India; Department of Medicine and Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom ABSTRACT A complete understanding of phagocytosis requires insight into both its biochemical and physical aspects. One of the ways to explore the physical mechanism of phagocytosis is to probe whether and how the target properties (e.g., size, shape, surface states, stiffness, etc.) affect their uptake. Here we report an imaging-based method to explore phagocytosis kinetics, which is compatible with real-time imaging and can be used to validate existing reports using fixed and stained cells. We measure single-event engulfment time from a large number of phagocytosis events to compare how size and shape of targets determine their engulfment. The data shows an increase in the average engulfment time for increased target size, for spherical particles. The uptake time data on nonspherical particles confirms that target shape plays a more dominant role than target size for phagocytosis: Ellipsoids with an eccentricity of 0.954 and much smaller surface areas than spheres were taken up five times more slowly than spherical targets. INTRODUCTION Macrophages can engulf a wide variety of targets, such as the degradation of phagosomal contents and the induction invading pathogens, dead cells, foreign airborne particles of the appropriate immune responses. -
Actin Nucleator Spire 1 Is a Regulator of Ectoplasmic Specialization in the Testis Qing Wen1,Nanli1,Xiangxiao 1,2,Wing-Yeelui3, Darren S
Wen et al. Cell Death and Disease (2018) 9:208 DOI 10.1038/s41419-017-0201-6 Cell Death & Disease ARTICLE Open Access Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis Qing Wen1,NanLi1,XiangXiao 1,2,Wing-yeeLui3, Darren S. Chu1, Chris K. C. Wong4, Qingquan Lian5,RenshanGe5, Will M. Lee3, Bruno Silvestrini6 and C. Yan Cheng 1 Abstract Germ cell differentiation during the epithelial cycle of spermatogenesis is accompanied by extensive remodeling at the Sertoli cell–cell and Sertoli cell–spermatid interface to accommodate the transport of preleptotene spermatocytes and developing spermatids across the blood–testis barrier (BTB) and the adluminal compartment of the seminiferous epithelium, respectively. The unique cell junction in the testis is the actin-rich ectoplasmic specialization (ES) designated basal ES at the Sertoli cell–cell interface, and the apical ES at the Sertoli–spermatid interface. Since ES dynamics (i.e., disassembly, reassembly and stabilization) are supported by actin microfilaments, which rapidly converts between their bundled and unbundled/branched configuration to confer plasticity to the ES, it is logical to speculate that actin nucleation proteins play a crucial role to ES dynamics. Herein, we reported findings that Spire 1, an actin nucleator known to polymerize actins into long stretches of linear microfilaments in cells, is an important regulator of ES dynamics. Its knockdown by RNAi in Sertoli cells cultured in vitro was found to impede the Sertoli cell tight junction (TJ)-permeability barrier through changes in the organization of F-actin across Sertoli cell cytosol. Unexpectedly, Spire 1 knockdown also perturbed microtubule (MT) organization in Sertoli cells cultured in vitro. -
Chloroplast Incorporation and Long-Term Photosynthetic Performance Through the Life Cycle in Laboratory Cultures of Elysia Timid
Chloroplast incorporation and long-term photosynthetic performance through the life cycle in laboratory cultures of Elysia timida (Sacoglossa, Heterobranchia) Schmitt et al. Schmitt et al. Frontiers in Zoology 2014, 11:5 http://www.frontiersinzoology.com/content/11/1/5 Schmitt et al. Frontiers in Zoology 2014, 11:5 http://www.frontiersinzoology.com/content/11/1/5 RESEARCH Open Access Chloroplast incorporation and long-term photosynthetic performance through the life cycle in laboratory cultures of Elysia timida (Sacoglossa, Heterobranchia) Valerie Schmitt1,2, Katharina Händeler2, Susanne Gunkel2, Marie-Line Escande3, Diedrik Menzel4, Sven B Gould2, William F Martin2 and Heike Wägele1* Abstract Introduction: The Mediterranean sacoglossan Elysia timida is one of the few sea slug species with the ability to sequester chloroplasts from its food algae and to subsequently store them in a functional state in the digestive gland cells for more than a month, during which time the plastids retain high photosynthetic activity (= long-term retention). Adult E. timida have been described to feed on the unicellular alga Acetabularia acetabulum in their natural environment. The suitability of E. timida as a laboratory model culture system including its food source was studied. Results: In contrast to the literature reporting that juvenile E. timida feed on Cladophora dalmatica first, and later on switch to the adult diet A. acetabulum, the juveniles in this study fed directly on A. acetabulum (young, non-calcified stalks); they did not feed on the various Cladophora spp. (collected from the sea or laboratory culture) offered. This could possibly hint to cryptic speciation with no clear morphological differences, but incipient ecological differentiation. -
Cell & Molecular Biology
BSC ZO- 102 B. Sc. I YEAR CELL & MOLECULAR BIOLOGY DEPARTMENT OF ZOOLOGY SCHOOL OF SCIENCES UTTARAKHAND OPEN UNIVERSITY BSCZO-102 Cell and Molecular Biology DEPARTMENT OF ZOOLOGY SCHOOL OF SCIENCES UTTARAKHAND OPEN UNIVERSITY Phone No. 05946-261122, 261123 Toll free No. 18001804025 Fax No. 05946-264232, E. mail [email protected] htpp://uou.ac.in Board of Studies and Programme Coordinator Board of Studies Prof. B.D.Joshi Prof. H.C.S.Bisht Retd.Prof. Department of Zoology Department of Zoology DSB Campus, Kumaun University, Gurukul Kangri, University Nainital Haridwar Prof. H.C.Tiwari Dr.N.N.Pandey Retd. Prof. & Principal Senior Scientist, Department of Zoology, Directorate of Coldwater Fisheries MB Govt.PG College (ICAR) Haldwani Nainital. Bhimtal (Nainital). Dr. Shyam S.Kunjwal Department of Zoology School of Sciences, Uttarakhand Open University Programme Coordinator Dr. Shyam S.Kunjwal Department of Zoology School of Sciences, Uttarakhand Open University Haldwani, Nainital Unit writing and Editing Editor Writer Dr.(Ms) Meenu Vats Dr.Mamtesh Kumari , Professor & Head Associate. Professor Department of Zoology, Department of Zoology DAV College,Sector-10 Govt. PG College Chandigarh-160011 Uttarkashi (Uttarakhand) Dr.Sunil Bhandari Asstt. Professor. Department of Zoology BGR Campus Pauri, HNB (Central University) Garhwal. Course Title and Code : Cell and Molecular Biology (BSCZO 102) ISBN : 978-93-85740-54-1 Copyright : Uttarakhand Open University Edition : 2017 Published By : Uttarakhand Open University, Haldwani, Nainital- 263139 Contents Course 1: Cell and Molecular Biology Course code: BSCZO102 Credit: 3 Unit Block and Unit title Page number Number Block 1 Cell Biology or Cytology 1-128 1 Cell Type : History and origin. -
Proteomic Analysis of Exosome-Like Vesicles Derived from Breast Cancer Cells
ANTICANCER RESEARCH 32: 847-860 (2012) Proteomic Analysis of Exosome-like Vesicles Derived from Breast Cancer Cells GEMMA PALAZZOLO1, NADIA NINFA ALBANESE2,3, GIANLUCA DI CARA3, DANIEL GYGAX4, MARIA LETIZIA VITTORELLI3 and IDA PUCCI-MINAFRA3 1Institute for Biomedical Engineering, Laboratory of Biosensors and Bioelectronics, ETH Zurich, Switzerland; 2Department of Physics, University of Palermo, Palermo, Italy; 3Centro di Oncobiologia Sperimentale (C.OB.S.), Oncology Department La Maddalena, Palermo, Italy; 4Institute of Chemistry and Bioanalytics, University of Applied Sciences Northwestern Switzerland FHNW, Muttenz, Switzerland Abstract. Background/Aim: The phenomenon of membrane that vesicle production allows neoplastic cells to exert different vesicle-release by neoplastic cells is a growing field of interest effects, according to the possible acceptor targets. For instance, in cancer research, due to their potential role in carrying a vesicles could potentiate the malignant properties of adjacent large array of tumor antigens when secreted into the neoplastic cells or activate non-tumoral cells. Moreover, vesicles extracellular medium. In particular, experimental evidence show could convey signals to immune cells and surrounding stroma that at least some of the tumor markers detected in the blood cells. The present study may significantly contribute to the circulation of mammary carcinoma patients are carried by knowledge of the vesiculation phenomenon, which is a critical membrane-bound vesicles. Thus, biomarker research in breast device for trans cellular communication in cancer. cancer can gain great benefits from vesicle characterization. Materials and Methods: Conditioned medium was collected The phenomenon of membrane release in the extracellular from serum starved MDA-MB-231 sub-confluent cell cultures medium has long been known and was firstly described by and exosome-like vesicles (ELVs) were isolated by Paul H. -
Nomina Histologica Veterinaria, First Edition
NOMINA HISTOLOGICA VETERINARIA Submitted by the International Committee on Veterinary Histological Nomenclature (ICVHN) to the World Association of Veterinary Anatomists Published on the website of the World Association of Veterinary Anatomists www.wava-amav.org 2017 CONTENTS Introduction i Principles of term construction in N.H.V. iii Cytologia – Cytology 1 Textus epithelialis – Epithelial tissue 10 Textus connectivus – Connective tissue 13 Sanguis et Lympha – Blood and Lymph 17 Textus muscularis – Muscle tissue 19 Textus nervosus – Nerve tissue 20 Splanchnologia – Viscera 23 Systema digestorium – Digestive system 24 Systema respiratorium – Respiratory system 32 Systema urinarium – Urinary system 35 Organa genitalia masculina – Male genital system 38 Organa genitalia feminina – Female genital system 42 Systema endocrinum – Endocrine system 45 Systema cardiovasculare et lymphaticum [Angiologia] – Cardiovascular and lymphatic system 47 Systema nervosum – Nervous system 52 Receptores sensorii et Organa sensuum – Sensory receptors and Sense organs 58 Integumentum – Integument 64 INTRODUCTION The preparations leading to the publication of the present first edition of the Nomina Histologica Veterinaria has a long history spanning more than 50 years. Under the auspices of the World Association of Veterinary Anatomists (W.A.V.A.), the International Committee on Veterinary Anatomical Nomenclature (I.C.V.A.N.) appointed in Giessen, 1965, a Subcommittee on Histology and Embryology which started a working relation with the Subcommittee on Histology of the former International Anatomical Nomenclature Committee. In Mexico City, 1971, this Subcommittee presented a document entitled Nomina Histologica Veterinaria: A Working Draft as a basis for the continued work of the newly-appointed Subcommittee on Histological Nomenclature. This resulted in the editing of the Nomina Histologica Veterinaria: A Working Draft II (Toulouse, 1974), followed by preparations for publication of a Nomina Histologica Veterinaria. -
Acanthamoeba Is an Evolutionary Ancestor of Macrophages: a Myth Or Reality? Ruqaiyyah Siddiqui Aga Khan University
eCommons@AKU Department of Biological & Biomedical Sciences Medical College, Pakistan February 2012 Acanthamoeba is an evolutionary ancestor of macrophages: A myth or reality? Ruqaiyyah Siddiqui Aga Khan University Naveed Ahmed Khan Aga Khan University Follow this and additional works at: http://ecommons.aku.edu/pakistan_fhs_mc_bbs Part of the Biochemistry Commons Recommended Citation Siddiqui, R., Khan, N. (2012). Acanthamoeba is an evolutionary ancestor of macrophages: A myth or reality?. Experimental Parasitology, 130(2), 95-97. Available at: http://ecommons.aku.edu/pakistan_fhs_mc_bbs/16 Experimental Parasitology 130 (2012) 95–97 Contents lists available at SciVerse ScienceDirect Experimental Parasitology journal homepage: www.elsevier.com/locate/yexpr Minireview Acanthamoeba is an evolutionary ancestor of macrophages: A myth or reality? ⇑ Ruqaiyyah Siddiqui a, Naveed Ahmed Khan a,b, a Aga Khan University, Stadium Road, Karachi, Pakistan b School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, England, UK article info abstract Article history: Given the remarkable similarities in cellular structure (morphological and ultra-structural features), Received 26 October 2011 molecular motility, biochemical physiology, ability to capture prey by phagocytosis and interactions with Received in revised form 17 November 2011 microbial pathogens, here we pose the question whether Acanthamoeba and macrophages are evolution- Accepted 19 November 2011 ary related. This is discussed in the light of evolution and functional aspects such as the astonishing Available online 28 November 2011 resemblance of many bacteria to infect and multiply inside human macrophages and amoebae in analo- gous ways. Further debate and studies will determine if Acanthamoeba is an evolutionary ancestor of Keywords: macrophages. Is this a myth or reality? Acanthamoeba Ó 2011 Elsevier Inc. -
Evidence for in Vivo Modulation of Chloroplast RNA Stability by 3-UTR Homopolymeric Tails in Chlamydomonas Reinhardtii
Evidence for in vivo modulation of chloroplast RNA stability by 3-UTR homopolymeric tails in Chlamydomonas reinhardtii Yutaka Komine*, Elise Kikis*, Gadi Schuster†, and David Stern*‡ *Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853; and †Department of Biology, Technion–Israel Institute of Technology, Haifa 32000, Israel Edited by Lawrence Bogorad, Harvard University, Cambridge, MA, and approved January 8, 2002 (received for review June 27, 2001) Polyadenylation of synthetic RNAs stimulates rapid degradation in synthesizes and degrades poly(A) tails (13). E. coli, by contrast, vitro by using either Chlamydomonas or spinach chloroplast extracts. encodes a poly(A) polymerase. These differences may reflect the Here, we used Chlamydomonas chloroplast transformation to test the evolution of the chloroplast to a compartment whose gene effects of mRNA homopolymer tails in vivo, with either the endog- regulation is controlled by the nucleus. enous atpB gene or a version of green fluorescent protein developed Unlike eukaryotic poly(A) tails, the roles of 3Ј-UTR tails in for chloroplast expression as reporters. Strains were created in which, prokaryotic gene expression are largely unexplored, although after transcription of atpB or gfp, RNase P cleavage occurred upstream they are widely distributed in microorganisms including cya- Glu of an ectopic tRNA moiety, thereby exposing A28,U25A3,[A؉U]26, nobacteria (14). Polyadenylated mRNAs have also been found in or A3 tails. Analysis of these strains showed that, as expected, both plant (15, 16) and animal mitochondria (17). The functions polyadenylated transcripts failed to accumulate, with RNA being of these tails have mostly been tested in vitro, which has undetectable either by filter hybridization or reverse transcriptase– highlighted RNA degradation but not interactions with other PCR. -
Exosome-Mediated Recognition and Degradation of Mrnas Lacking A
R EPORTS wild-type PGK1 mRNA was synthesized with a poly(A) tail of approximately 70 residues and Exosome-Mediated Recognition was subsequently deadenylated slowly (Fig. 2A) (12). In contrast, nonstop-PGK1 transcripts dis- and Degradation of mRNAs appeared rapidly without any detectable dead- enylation intermediates (Fig. 2B). In addition, in Lacking a Termination Codon a ski7⌬ strain, the nonstop mRNA persisted as a fully polyadenylated species for 8 to 10 min Ambro van Hoof,1,2* Pamela A. Frischmeyer,1,3 Harry C. Dietz,1,3 before disappearing (Fig. 2C). These data indi- Roy Parker1,2* cate that exosome function is required for rapid degradation of both the poly(A) tail and the One role of messenger RNA (mRNA) degradation is to maintain the fidelity of body of the mRNA. Based on these observa- gene expression by degrading aberrant transcripts. Recent results show that tions, we suggest that nonstop mRNAs are rap- mRNAs without translation termination codons are unstable in eukaryotic cells. idly degraded in a 3Ј-to-5Ј direction by the We used yeast mutants to demonstrate that these “nonstop” mRNAs are exosome, beginning at the 3Ј end of the poly(A) degraded by the exosome in a 3Ј-to-5Ј direction. The degradation of nonstop tail (13). transcripts requires the exosome-associated protein Ski7p. Ski7p is closely Two observations suggest a mechanism by related to the translation elongation factor EF1A and the translation termi- which nonstop mRNAs are specifically recog- nation factor eRF3. This suggests that the recognition of nonstop mRNAs nized and targeted for destruction by the exo- involves the binding of Ski7p to an empty aminoacyl-(RNA-binding) site (A site) some.