The Next Generation www.ems-us.org

EMS President: Catherine B. Klein

Program Chair: Mats Ljungman

Keynote Lecturers: Judith Campisi Jan H.J. Hoeijmakers Leroy Hood Mary-Claire King William J. Martin II Yosef Shiloh John A. Stamatoyannopoulos

EMS Award Recipient: rd Susan S. Wallace 43 Annual Meeting Program

Scan Me!

Access the EMS Bellevue, Washington Annual Meeting website at www.ems-us.org/am2012/index.asp, or Scan this QR code with your smartphone Eastside of Seattle at the Hyatt Regency Bellevue or mobile device to access the site September 8–12, 2012 directly. The QR Code can be decoded by most camera-equipeed smarthphoes with a free downloaded QR code reader application. Sponsors of the 43rd Annual Meeting

Platinum March of Dimes Foundation Grant 4-FY12-536 National Institute of Environmental Health Sciences, NIH R13 Grant 1R13ES022128-01 Gold Abbott Laboratories Illumina EMS Executive Board Janssen Pharmaceutical Companies

Silver Agilent Technologies Miltenyi Biotec Amgen Inc. Molecular Toxicology, Inc. BioReliance Corporation National Institute of Environmental Health Sciences Boehringer Ingelheim Pharmaceuticals Pfizer Global Research and Development Bristol-Myers Squibb Sanofi Genetic Toxicology Association Society of Toxicology ILSI-HESI IVGT Committee WIL Research John Wiley & Sons, Inc. Litron Laboratories Bronze

Environmental and Molecular Mutagenesis John J. Nicolette Editorial Office EpiGenie.com 43rd Annual Meeting Program

Bellevue, Washington Eastside of Seattle at the Hyatt Regency Bellevue September 8–12, 2012

Program Chair: Mats Ljungman, PhD Abstracts printed in Environmental and Molecular Mutagenesis Volume 53, Number S1 The Environmental Mutagen Society was founded in 1969 and is incorporated under the laws of the District of Columbia. Its purpose is to encourage the study of mutagens in the human environment, particularly as they may affect public health, and to engage in and sponsor research and the dissemination of information related to mutagens. Membership is open to all interested scientists. All text and graphics are copyright © 2012 by the Environmental Mutagen Society and may not be used or duplicated without written consent from the Society. MEETING OVERVIEW 43rd Annual Meeting of the Environmental Mutagen Society MEETING OVERVIEWMEETING

Table of Contents Meeting Overview

Meeting Overview...... 2 Friday, September 7 Leadership Officers...... 5 4:00 PM–6:00 PM Grand Staircase Landing (2nd Floor) Councilors...... 5 REGISTRATION Past Presidents...... 5 Headquarters Staff...... 5 Saturday, September 8 Committees...... 6 Table of Contents Special Interest Group Leaders...... 8 7:00 AM–6:00 PM GRAND BALLROOM FOYER Council Task Forces...... 8 REGISTRATION Events 7:00 AM–6:00 PM Green room (3rd floor) Committee Meetings ...... 9 SPEAKER READY ROOM OPEN Special Interest Group Meetings...... 9 Welcome Reception and Student and 8:00 AM–12:00 Noon AUDITORIUM New Investigator Poster Session...... 9 WORKSHOP 1 The EMS Bowling Challenge...... 9 New Developments in Genetic Toxicology Testing: The Pig-a Assay Business Meeting and Town Hall...... 9 9:30 AM–12:00 Noon LAUREL President’s Reception ...... 9 EMS EXECUTIVE BOARD MEETING Future Meetings...... 9 12:00 Noon–1:00 PM Sponsored Events...... 10 LUNCH ON YOUR OWN General Information Welcome to the 43rd 1:00 PM–5:00 PM LARCH EMS Annual Meeting...... 11 EMS COUNCIL MEETING Meeting Highlights...... 11 1:00 PM–5:00 PM AUDITORIUM Registration Hours...... 11 WORKSHOP 2 Registration Materials...... 11 New Developments in Genetic Toxicology: Automated In Vitro Exhibit and Poster Hours...... 11 Micronucleus Assay Author Attended Poster Hours...... 11 1:00 PM–5:00 PM GRAND BALLROOM A Poster Presentations...... 11 WORKSHOP 3 Photography Policy Next Generation Technologies during Scientific Sessions...... 11 First Aid and Security...... 11 5:00 PM–7:00 PM EVERGREEN BALLROOM E About Safety and Security...... 12 WELCOME RECEPTION AND STUDENT AND NEW INVESTIGATOR POSTER SESSION Internet Access at the Annual Meeting ...... 12 Conference Site...... 12 7:00 PM–9:10 PM GRAND BALLROOM A Local Transportation ...... 12 OPENING KEYNOTE SESSION Hotel Parking...... 12 7:00 PM–7:10 PM Meals...... 12 Welcome and Opening of the Annual Meeting Agenda Mats Ljungman, The University of Michigan Friday ...... 13 7:10 PM–8:10 PM Saturday ...... 13 KEYNOTE LECTURE 1 Sunday...... 14 Genomic Analysis of Mutations That Appear “Out of the Blue” Monday...... 20 Lecturer: Mary-Claire King, University of Washington Tuesday...... 26 8:10 PM–9:10 PM Wednesday...... 29 KEYNOTE LECTURE 2 Exhibits...... 32 The Key Role of DNA Damage on Cancer, Aging, and Longevity Lecturer: Jan H.J. Hoeijmakers, Erasmus University Maps of Hyatt Regency Bellevue...... 34

2 www.ems-us.org Bellevue, Washington • September 8–12, 2012 MEETING OVERVIEW

SUNday, September 9 8:00 PM–10:00 PM Lucky Strike Lanes THE EMS BOWLING CHALLENGE 7:00 AM–5:00 PM GRAND BALLROOM FOYER

REGISTRATION MEETING OVERVIEW MONDAY, September 10 7:00 AM–5:00 PM GRAND BALLROOM E 7:00 AM–5:00 PM Grand Ballroom Foyer SPEAKER READY ROOM OPEN REGISTRATION 7:00 AM–8:30 AM BREAKFAST MEETINGS 7:00 AM–5:00 PM GRAND BALLROOM E Breakfast Pickup for Meetings EVERGREEN BALLROOM FOYER (Ticket Required—Breakfast Available at 6:45 AM) SPEAKER READY ROOM OPEN Applied Genetic Toxicology SIG EVERGREEN BALLROOM A 7:00 AM–8:30 AM BREAKFAST MEETINGS DNA Repair and Mutagenic Mechanisms SIG EVERGREEN BALLROOM B Breakfast Pickup for Meetings EVERGREEN BALLROOM FOYER (Ticket Required—Breakfast Available at 6:45 AM) Heritable Mutation and Disease SIG EVERGREEN BALLROOM C Epigenetics SIG EVERGREEN BALLROOM A 8:30 AM–9:30 AM GRAND BALLROOM I Molecular Epidemiology SIG EVERGREEN BALLROOM B KEYNOTE LECTURE 3 New Technologies SIG EVERGREEN BALLROOM C Cancer and Aging: Rival Demons Linked by Inflammation? Lecturer: Judith Campisi, Buck Institute for Age Research 8:30 AM–6:00 PM EVERGREEN BALLROOM E 9:45 AM–12:30 PM GRAND BALLROOM A POSTERS AND EXHIBITS OPEN SYMPOSIUM 1 Inflammation, Cancer, and Aging 8:30 AM–9:30 AM GRAND BALLROOM I KEYNOTE LECTURE 4 9:45 AM–12:30 PM GRAND BALLROOM I Epigenomics: Reading the Living Human Genome SYMPOSIUM 2 Lecturer: John Stamatoyannopoulos, University of Washington Emerging Approaches in Predictive Toxicology 9:45 AM–12:30 PM GRAND BALLROOM A 12:30 PM–1:45 PM EVERGREEN BALLROOM A SYMPOSIUM 3 EMM EDITORIAL BOARD MEETING Next Generation Environmental Epigenetics 12:30 PM–2:00 PM 9:45 AM–12:30 PM GRAND BALLROOM I LUNCH ON YOUR OWN SYMPOSIUM 4 Next Generation Risk Assessment and Regulatory Policies 12:30 PM–2:00 PM EVERGREEN BALLROOM C STUDENT AND NEW INVESTIGATOR LUNCHEON 12:30 PM–12:45 PM GRAND BALLROOM FOYER (Advance Registration Required) BOXED LUNCH PICKUP (Ticket Required—For the EMS Business Meeting and Town Hall) 2:00 PM–4:00 PM GRAND BALLROOM A PLATFORM SESSION 1 12:45 PM–2:00 PM GRAND BALLROOM I DNA Repair BUSINESS MEETING AND TOWN HALL (Boxed Lunches Available) 2:00 PM–4:00 PM GRAND BALLROOM I 2:00 PM–4:00 PM GRAND BALLROOM a PLATFORM SESSION 2 The Environment, Genes, and Public Health PLATFORM SESSION 3 Epigenetics and the DNA Damage Response 4:00 PM–6:00 PM EVERGREEN BALLROOM E 2:00 PM–4:00 PM GRAND BALLROOM i POSTERS SESSION 1 AND EXHIBITS Odd Numbered Posters Attended PLATFORM SESSION 4 Genotoxicity and Toxicogenomic Analyses of Environmental 6:00 PM–7:00 PM COMMITTEE MEETINGS Contaminants 2013 Program Committee (First Meeting) Larch 4:00 PM–6:00 PM EVERGREEN BALLROOM E Awards and Honors Committee Juniper POSTERS SESSION 2 AND EXHIBITS Public Relations and Communications Committee Madrona Even Numbered Posters Attended Publication Policy Committee Laurel 6:00 PM–8:00 PM DINNER ON YOUR OWN www.ems-us.org 3 MEETING OVERVIEW 43rd Annual Meeting of the Environmental Mutagen Society MEETING OVERVIEWMEETING

6:00 PM–7:00 PM COMMITTEE MEETINGS 2:00 PM–4:00 PM GRAND BALLROOM I Alexander Hollaender Outreach Committee Juniper PLATFORM SESSION 6 Chromosome Instability Education, Student and New Investigator Affairs Committee Larch Membership and Professional Development Committee Laurel 4:15 PM–5:15 pM GRAND BALLROOM I KEYNOTE LECTURE 6 7:00 PM–8:00 PM The Emergence of Proactive P4 Medicine: A Revolution in Special Interest Group Leaders Laurel Healthcare Lecturer: Leroy Hood, Institute for Systems Biology 8:00 PM–10:30 PM BELLEVUE ARTs MUSEUM PRESIDENT’S RECEPTION 5:15 PM–6:15 pM GRAND BALLROOM I (Open to All Meeting Registrants) EMS AWARD LECTURE Oxidative DNA Damages: Search and Destroy Lecturer: Susan S. Wallace, University of Vermont Tuesday, September 11 7:00 PM–12:00 Midnight Grand Ballroom E 7:00 AM–5:00 PM GRAND BALLROOM FOYER BANQUET REGISTRATION Awards Presentation by Catherine B. Klein 7:00 AM–5:00 PM GRAND BALLROOM E Alexander Hollaender Award SPEAKER READY ROOM OPEN EMS Award EMS Service Award 7:00 AM–8:30 AM Larch EMS Emerging Scientist Travel Awards EMS EXECUTIVE BOARD MEETING Student and New Investigator Travel Awards

7:00 AM–8:30 AM BREAKFAST MEETINGS Wednesday, September 12 Breakfast Pickup for Meetings EVERGREEN BALLROOM FOYER (Ticket Required—Breakfast Available at 6:45 AM) 7:00 AM–11:30 AM Grand BALLROOM FOYER Environmental Genetic Toxicology SIG EVERGREEN BALLROOM A REGISTRATION

Risk Assessment SIG EVERGREEN BALLROOM B 7:00 AM–11:00 AM GRAND BALLROOM E Transgenic and In Vivo Mutagenesis SIG EVERGREEN BALLROOM C SPEAKER READY ROOM OPEN

8:30 AM–12:00 NOON EVERGREEN BALLROOM E 7:00 AM–8:30 AM COMMITTEE MEETINGS POSTERS AND EXHIBITS OPEN 2013 Program Committee (Second Meeting) Larch 8:30 AM–9:30 AM GRAND BALLROOM I Finance and Resource Committee Laurel KEYNOTE LECTURE 5 Nominating Committee Juniper The ATM-Mediated DNA Damage Response: Moving between the Forest and the Trees 8:30 AM–11:15 AM GRAND BALLROOM A Lecturer: Yosef Shiloh, Tel Aviv University SYMPOSIUM 7 Management of the DNA Damage Response 9:45 AM–12:30 PM GRAND BALLROOM A SYMPOSIUM 5 8:30 AM–11:15 AM GRAND BALLROOM I DNA Damage-Mediated Gene Regulation SYMPOSIUM 8 Health Effects in Heavily Polluted Areas 9:45 AM–12:30 PM GRAND BALLROOM I SYMPOSIUM 6 11:30 AM–12:30 PM GRAND BALLROOM I Mechanisms of Germ Cell Aneuploidy: Do Environmental KEYNOTE LECTURE 7 Exposures Play a Role? Household Air Pollution: A Preventable Cause of Death for Two Million People Annually 12:30 PM–2:00 PM Lecturer: William J. Martin II, National Institutes of Health LUNCH ON YOUR OWN 12:30 PM–12:35 PM GRAND BALLROOM I 12:30 PM–2:00 PM EVERGREEN BALLROOM A CLOSE OF THE MEETING WOMEN IN THE EMS SPECIAL INTEREST GROUP Mats Ljungman, The University of Michigan LUNCH MEETING (Advance Registration Required) 2:00 PM–5:00 PM LARCH COUNCIL MEETING 2:00 PM–4:00 PM GRAND BALLROOM A PLATFORM SESSION 5 Mutagenesis, Environmental Genomics, and Cancer

4 www.ems-us.org Bellevue, Washington • September 8–12, 2012 EMS LEADERSHIP

EMS Officers

Catherine B. Klein, PhD, President Suzanne M. Morris, PhD, Secretary New York University School of Medicine National Center for Toxicological Research, US FDA

Mats Ljungman, PhD, President-Elect Ofelia A. Olivero, PhD, President-Elect (2012–2013) EMS LEADERSHIP The University of Michigan National Cancer Institute, NIH Barbara S. Shane, PhD, DABT, Treasurer Barbara Shane Consulting

EMS Councilors

Andrew B. Buermeyer, PhD (2012) Janet E. Baulch, PhD (2013) Graciela Spivak, PhD (2014) Krista L. Dobo, PhD (2012) Stephen D. Dertinger, PhD (2013) Kevin S. Sweder, PhD (2014) Patricia A. Escobar, PhD (2012) Christopher M. Somers, PhD (2013) Hilde E. van Gijssel, PhD (2014) Francesco Marchetti, PhD (2012) Kandace J. Williams, PhD (2013) David M. Wilson III, PhD (2014) Thomas E. Wilson, MD, PhD (2012) Kristine L. Witt, MS (2014)

Past Presidents

Alexander Hollaender, PhD (1970) John A. Heddle, PhD (1986) James S. Felton, PhD (1999) Ernst Freese, PhD (1972) Anthony V. Carrano, PhD (1987) James D. Tucker, PhD (2000) Frederick J. de Serres, PhD (1974) Sheila M. Galloway, PhD (1988) David M. DeMarini, PhD (2001) Jan Drake, PhD (1976) R. Julian Preston, PhD (1989) Lawrence A. Loeb, MD, PhD (2002) Seymour Abrahamson, PhD (1977) George R. Hoffmann, PhD (1990) David A. Eastmond, PhD (2003) David J. Brusick, PhD, ATS (1978) Michael D. Waters, PhD (1991) Leona D. Samson, PhD (2004) Mortimer L. Mendelsohn, MD, PhD (1979) James T. MacGregor, PhD, DABT (1992) Peter J. Stambrook, PhD (2005) Sheldon Wolff, PhD (1980) Philip C. Hanawalt, PhD (1993) Martina L. Veigl, PhD (2006) Verne A. Ray, PhD (1981) James M. Gentile, PhD (1994) Andrew J. Wyrobek, PhD (2007) Gordon W. Newell, PhD (1982) Raymond R. Tice, PhD (1995) Priscilla K. Cooper, PhD (2008) Richard J. Albertini, MD, PhD (1983) Raymond W. Tennant, PhD (1996) Michael J. Plewa, PhD (2009) Liane B. Russell, PhD (1984) Sid Aaron, PhD, DABT (1997) Jeffrey L. Schwartz, PhD (2010) Michael D. Shelby, PhD (1985) Rosalie K. Elespuru, PhD (1998)

Headquarters Staff

Tonia M. Masson, Executive Director Maureen Kettering, Meetings and Exhibits Manager Becca Isakower, Program Manager Rachel Frohberg, Program Coordinator

www.ems-us.org 5 EMS LEADERSHIP 43rd Annual Meeting of the Environmental Mutagen Society EMS LEADERSHIP Committees

Alexander Hollaender Outreach Philip C. Hanawalt, PhD, Chair David M. DeMarini, PhD Peter J. Stambrook, PhD Graciela Spivak, PhD, Co-Chair James Gentile, PhD Paul A. White, PhD William W. Au, PhD Helena Groot de Restrepo, PhD Mats Ljungman, PhD, Past Chair Priscilla K. Cooper, PhD Regina D. Montero-Montoya, PhD Meredith E. Crosby, PhD Delbert M. Shankel, PhD Awards and Honors Richard D. Wood, PhD, Chair Patricia L. Opresko, PhD Jeffrey L. Schwartz, PhD Karen M. Vasquez, PhD, Co-Chair Barbara L. Parsons, PhD Paul A. White, PhD Joel N. Meyer, PhD Jennifer C. Sasaki, PhD Education, Student and New Investigator Affairs Hilde E. van Gijssel, PhD, Chair Meredith E. Crosby, PhD Meagan Myers Stephanie L. Smith-Roe, PhD, Co-Chair Patricia A. Escobar, PhD Ofelia A. Olivero, PhD Jeffrey C. Bemis, PhD Glenda J. Gentile Senthilkumar Perumal Kuppusamy Amanda Smith Bess Sabine S. Lange, PhD Janice M. Pluth, PhD Javed A. Bhalli, PhD Suzanne M. Morris, PhD Zorica Scuric, PhD Guosheng Chen, PhD John J. Mulvihill, MD Jeffrey Stumpf, PhD

FASEB Representatives Laura J. Niedernhofer, MD, PhD Joann B. Sweasy, PhD Karen M. Vasquez, PhD FASEB Board Representative, The FASEB Journal Editorial Board Peer Review Subcommittee Public Affairs Committee Representative

Finance and Resource Andrew D. Kligerman, PhD, Chair E. Maria Donner, PhD Jeffrey L. Schwartz, PhD Barbara S. Shane, PhD, DABT, Treasurer Barbara L. Parsons, PhD Carol R. Tometsko Jack B. Bishop, PhD Michael J. Plewa, PhD

Membership and Professional Development Kandace J. Williams, PhD, Chair Michael A. Edelbrock, PhD Jonathan B. Ward Jr., PhD Sandy K. Weiner, MS, Co-Chair Catherine B. Klein, PhD Andrew J. Wyrobek, PhD Junjian Chen, PhD Suzanne M. Morris, PhD Special Interest Group Leaders Priscilla K. Cooper, PhD Michael J. Plewa, PhD E. Maria Donner, PhD Jeffrey L. Schwartz, PhD

Nominating Michael J. Plewa, PhD, Chair Stephanie L. Smith-Roe, PhD Jeffrey L. Schwartz, PhD, Co-Chair Ronald D. Snyder, PhD Krista L. Dobo, PhD Kevin S. Sweder, PhD Sheila M. Galloway, PhD Gregory L. Erexson, PhD, DABT Miriam C. Poirier, PhD Parliamentarian

6 www.ems-us.org Bellevue, Washington • September 8–12, 2012 EMS LEADERSHIP

Program Mats Ljungman, PhD, Chair Mugimane G. Manjanatha, PhD Christopher M. Somers, PhD

Janet E. Baulch, PhD Francesco Marchetti, PhD Joann B. Sweasy, PhD EMS LEADERSHIP Jack B. Bishop, PhD Ofelia A. Olivero, PhD Sundar Venkatachalam Krista L. Dobo, PhD Stefan J. Pfuhler, PhD Kristine L. Witt, MS Catherine F. Gibbons, PhD Miriam C. Poirier, PhD Carole L. Yauk, MSc, PhD Nagu Keshava, PhD Barbara S. Shane, PhD, DABT Catherine B. Klein, PhD Ronald D. Snyder, PhD

Public Relations and Communications Jeffrey K. Wickliffe, PhD, Chair Daneida Lizarraga Lopez, PhD, MD Jeffrey L. Schwartz, PhD Carlos A. Torres-Ramos, PhD, Co-Chair Mugimane G. Manjanatha, PhD, Past Chair Sandy K. Weiner, MS Michelle C. DeSimone Meagan Myers Suzanne M. Morris, PhD Masamitsu Honma, PhD Patricia Ostrosky-Wegman, PhD James H. Kim, PhD Barbara L. Parsons, PhD

Publication Policy Stephen D. Dertinger, PhD, Chair Malcolm J. Lippert, PhD David M. Wilson III, PhD, Associate Editor James D. Tucker, PhD, Co-Chair Francesco Marchetti, PhD, Editor-in-Chief Carole L. Yauk, MSc, PhD, Associate Editor Larry D. Claxton, PhD Ronald D. Snyder, PhD Paul A. White, PhD Sheila M. Galloway, PhD Kristine L. Witt, MS Robert H. Heflich, PhD Errol Zeiger, PhD, JD, ATS

Scientific Liaison Coalition Rosalie K. Elespuru, PhD Ofelia A. Olivero, PhD Catherine F. Gibbons, PhD Jeffrey L. Schwartz, PhD

Web Site R. Daniel Benz, PhD Hilde E. van Gijssel, PhD Margaret Pratt, PhD Jack B. Bishop, PhD Mats Ljungman, PhD Jeffrey L. Schwartz, PhD Bevin P. Engelward, ScD John J. Nicolette, MS, DABT John A. Tainer, PhD Barry N. Ford, PhD Ofelia A. Olivero, PhD Martina L. Veigl, PhD

www.ems-us.org 7 EMS LEADERSHIP 43rd Annual Meeting of the Environmental Mutagen Society EMS LEADERSHIP Special Interest Group Leaders

Applied Genetic Toxicology Heritable Mutation Transgenic and In Vivo Krista L. Dobo, PhD and Disease Mutagenesis John J. Nicolette, MS, DABT Francesco Marchetti, PhD Mugimane G. Manjanatha, PhD Christopher M. Somers, PhD Nan Mei, PhD DNA Repair and Mutagenic Carole L. Yauk, MSc, PhD Mechanisms Women in the EMS Robert W. Sobol Jr., PhD Molecular Epidemiology Barbara L. Parsons, PhD Joann B. Sweasy, PhD Miriam C. Poirier, PhD Kristine L. Witt, MS Radim J. Sram, MD, DSc Environmental Genetic SIG Committee Chairs Toxicology New Technologies Ofelia A. Olivero, PhD, Chair Christopher M. Somers, PhD Sundar Venkatachalam Carole L. Yauk, MSc, PhD, Co-Chair Carol D. Swartz, DVM, PhD Risk Assessment Epigenetics David A. Eastmond, PhD Janet E. Baulch, PhD Nagu Keshava, PhD Dana C. Dolinoy, PhD Daneida Lizarraga Lopez, PhD, MD

Council Task Forces Brand Identity Task Force Endowment Task Force Outreach Task Force Thomas E. Wilson, MD, PhD, Chair Ronald D. Snyder, PhD, Chair John J. Nicolette, MS, DABT, Chair David M. DeMarini, PhD Jeffrey L. Schwartz, PhD Patricia A. Escobar, PhD Stephen D. Dertinger, PhD Barbara S. Shane, PhD, DABT Nagu Keshava, PhD Bevin P. Engelward, ScD Graciela Spivak, PhD Catherine B. Klein, PhD Philip C. Hanawalt, PhD David M. Wilson III, PhD Mats Ljungman, PhD James T. MacGregor, PhD, DABT Ofelia A. Olivero, PhD Stephanie L. Smith-Roe, PhD Karren Hven Northgeta, PhD Kristine L. Witt, MS Sandy K. Weiner, MS Carole L. Yauk, MSc, PhD Kandace J. Williams, PhD

8 www.ems-us.org Bellevue, Washington • September 8–12, 2012 EMS EVENTS

EMS Events

EMS Committee Meetings The EMS Bowling Challenge EMS EVENTS The EMS committees are active throughout the year and have at Sunday Evening least one organized meeting during the conference. The committee On Sunday evening at 8:00 pm you will have a chance to show off meeting dates and times are noted in the program. Members are your bowling talents during the “EMS Bowling Challenge”. We will encouraged to be involved in a committee. Contact the committee be bowling at the upscale “Lucky Strike Lanes” (Visit: chair if you are interested in serving on a committee. www.bowlluckystrike.com/locations/washington/bellevue) which is just next door to the Hyatt Regency Hotel. We will have some EMS Special Interest Group Meetings friendly competition between the different SIGs. The breakfast meetings of the Society SIGs are a time-tested favorite of the Annual Meeting. The format provides free-form discussions Business Meeting and Town Hall and short presentations of key challenges and new initiatives. The Monday Afternoon SIGs provide a casual way for young investigators and seasoned researchers to interact. The Business Meeting and Town Hall will take place on Monday at 12:45 pm. Plan to attend this meeting to obtain a brief The SIG breakfast meetings are scheduled Sunday through Tuesday overview of accomplishments which will be shared by the EMS as noted in the program. The Women in EMS SIG is presenting a leadership and EIC. (Committee reports will be available via EMS lunchtime meeting on Tuesday at 12:30 pm. Separate registration is Connect.) The 2012 Town Hall will provide a forum to report the required for the SIG meetings. recommendations of the of the Brand Identity Task Force and EMS leadership. Welcome Reception and Student and New Investigator Poster Session President’s Reception at Bellevue Arts Museum Saturday Afternoon Monday Evening The Welcome Reception will take place at 5:00 pm on Saturday in This event will take place from 8:00 pm–10:30 pm at the wonderful Evergreen Ballroom E. The Student and New Investigator Poster contemporary Bellevue Arts Museum located just a block from the Session is part of the Welcome Reception. All students and new Hyatt Regency Hotel. All meeting registrants are invited to attend. investigators presenting during the meeting should put their poster Coffee and desserts will be served and the whole museum is ours to on display—it is one more opportunity to highlight your research enjoy. and is in addition to your scheduled presentation. The Evergreen Ballroom is located on the first floor. See diagram on page 34.

Future Meetings (Occur in September) EMS 44th Annual Meeting EMS 46th Annual Meeting September 21–25, 2013 September 26–30, 2015 Hyatt Regency Monterey Sheraton New Orleans Monterey, California New Orleans, Louisiana EMS 45th Annual Meeting September 13–17, 2014 Hilton at Disney World Resort Orlando, Florida

www.ems-us.org 9 SPONSORED EVENTS 43rd Annual Meeting of the Environmental Mutagen Society SPONSORED EVENTSS Sponsored Events

Saturday, September 8 Tuesday, September 11

Workshop 1: New Developments in Genetic Symposium 6: Mechanisms of Germ Toxicology Testing I: The Pig-a Assay Cell Aneuploidy: Do Environmental Contributing Sponsors: Bristol-Myers Squibb, Exposures Play a Role? Litron Laboratories, and Miltenyi Biotec Primary Sponsor: March of Dimes Foundation Grant Supported in Part by: BioReliance Corporation, 4-FY12-536 Genetic Toxicology Association, and John J. Nicolette Women in the EMS Special Interest Group Lunch Meeting Workshop 2: New Developments in Genetic Toxicology: Automated In Vitro Contributing Sponsor: National Institute of Micronucleus Assay Environmental Health Sciences Primary Sponsors: Pfizer Global Research and Development and WIL Research general meeting sponsors Contributing Sponsors: Litron Laboratories Supported in Part by: BioReliance Corporation, Abbott Laboratories Genetic Toxicology Association, and John J. Nicolette Amgen Inc. Workshop 3: Next Generation Technologies Boehringer Ingelheim Pharmaceuticals Primary Sponsor: Illumina EpiGenie.com Contributing Sponsor: Agilent Technologies WELCOME RECEPTION AND Student and Environmental and Molecular Mutagenesis NEW INVESTIGATOR POSTER SESSION Editorial Office Supported in part by: Genetic Toxicology Association Janssen Pharmaceutical Companies Sunday, September 9 John Wiley & Sons, Inc. The EMS Bowling Challenge Supported in Part by: BioReliance Corporation Molecular Toxicology, Inc.

Monday, September 10 National Institute of Environmental Health Sciences, NIH R13 Grant 1R13ES022128-01 Symposium 4: Next Generation Risk Assessment and Regulatory Policies Sanofi Supported in Part By: ILSI-HESI IVGT Committee President’s reception Society of Toxicology Contributing Sponsor: EMS Executive Board Student and New Investigator Travel Awards

National Institute of Environmental Health Sciences, NIH R13 Grant 1R13ES022128-01

10 www.ems-us.org Bellevue, Washington • September 8–12, 2012 GENERAL INFORMATION

General Information

Welcome to the 43rd EMS Annual Meeting! Registration Materials EMS was founded to provide a forum for the establishment and The registration reference materials are posted online at support of scientists in the field of environmental mutagenesis. www.ems-us.org/am2012/onlineregkit.asp. You must log in to Over the past 43 years our society has helped shape public access the materials which are restricted to meeting registrants. policy on the environment by providing critical information You will find useful information about late-breaking abstracts, GENERAL INFORMATION on environmental mutagens. While the initial focus of EMS sponsors, exhibitors, the registrant list, and more. Please stop was on germ-cell mutagenesis, which remains an area of strong by the Registration Desk if you need your login and password interest, today the EMS is also the primary professional society for information. scientists involved in understanding mechanisms of DNA repair, mutagenesis, and the regulation of the epigenome, environmental Exhibit and Poster Hours genomics, consequences of mutation, such as cancer, and Sunday 4:00 PM–6:00 PM the evaluation of risk from genotoxic agents for mutagenesis, carcinogenesis, birth defects, and other disorders. Monday 8:30 AM–6:00 PM Tuesday 8:30 AM–12:00 Noon Meeting Highlights This year’s scientific program consists of eight symposia, seven Attended Poster Hours plenary lectures, three workshops, two poster sessions, and six Sunday 4:00 PM–6:00 PM platform sessions. In addition to the scientific program, the Annual Odd Numbered Posters Attended Meeting will convene meetings of the ten Special Interest Groups (SIGs) that represent the diversity of the Society and help set the Monday 4:00 PM–6:00 PM agenda for future EMS meetings. Don’t miss the opportunity to Even Numbered Posters Attended participate in a discussion about the recommendations of the Brand Identity Task Force and EMS leadership during the Business Poster Presentations Meeting and Town Hall on Monday. Be sure to play for your SIG The posters will be displayed in Evergreen Ballroom E. Posters team at the “EMS Bowling Challenge” on Sunday evening, attend should be affixed between 12:00 noon–3:30 pm on Sunday, the President’s Reception at Bellevue Arts Museum on Monday September 9. Each poster should be displayed in the area night, and the Annual Meeting Banquet on Tuesday evening, which corresponding with the assigned poster number. All posters will include award presentations, good food, great company, and will be on display continuously from Sunday through Tuesday. lots of dancing. Poster presenters are requested to attend their posters during Meeting attendees who are interested in bringing a guest to the their assigned poster session; this is when conference participants Welcome Reception, President’s Reception and/or the Banquet can will plan to meet you to discuss your work. All posters must be purchase tickets for these events at the Registration Desk. Guest removed on Tuesday, September 11 between 12:00 noon and tickets for all three events are $150, tickets for individual events are 1:00 pm. also available. Please note badges are required for all events and sessions. Photography Policy during Scientific Sessions Photography and recording of scientific presentations is prohibited Registration without advance specific consent of the presenter(s)/author(s). The Registration Desk hours and locations are listed below. Both Session Chairs are asked to strictly enforce this policy and the Grand Staircase Landing and the Grand Ballroom Foyer are individuals who do not comply will be asked to leave the session. located on the second floor. See diagram on page 35. In addition, cameras and recording devices are prohibited in the Exhibit Hall. Grand Staircase Landing Friday 4:00 PM–6:00 PM First Aid and Security Grand Ballroom Foyer The Hyatt Regency Bellevue has equipped meeting rooms and foyers with house phones to use in case of an emergency. If you Saturday 7:00 AM–6:00 PM need medical or security assistance, pick up a house phone, dial 55, Sunday 7:00 AM–5:00 PM and the hotel operator will connect you to the correct department. Monday 7:00 AM–5:00 PM Tuesday 7:00 AM–5:00 PM Wednesday 7:00 AM–11:30 AM www.ems-us.org 11 GENERAL INFORMATION 43rd Annual Meeting of the Environmental Mutagen Society GENERAL INFORMATIONGENERAL

About Safety and Security “new world flavors” in a hip atmosphere, Zen Express featuring casual Asian cuisine, the Needs Deli, as well as over 45 restaurants There is a possibility of demonstrators given the nature of connected to the hotel via a series of sky bridges. our conference. Instances of this nature range from verbal confrontations, protest, or strikes. We recommend the following This facility offers complimentary 24-hour access to a 7,000 square procedures in the event of demonstrations: foot Hyatt StayFit gym, heated 25-meter lap pool, and nearby Bellevue Downtown Park. • Wear your name badge in the Hyatt Regency Bellevue. When leaving the hotel, remove it so as to blend with other people. The scientific sessions and meetings are located on the lobby level, second floor, and third floor. See the diagram on page 35. • If you see a demonstration or protest beginning, please contact a member of the EMS staff and they will initiate the appropriate response. If you see actions that appear threatening, notify the Local Transportation nearest security officer. Downtown Seattle can be conveniently reached in 20 minutes by express bus 550 leaving every 15 minutes from Bellevue Transit • Do not engage, defend either side, or subdue person(s) in any Center, a few blocks from the Hyatt Regency hotel (fare: $2). For type of disturbance. Demonstrators are usually trying to attract more information about the Seattle area public transit system please media attention. Don’t help them. visit www.ems-us.org/AM2012/transportation.asp. • EMS representatives will respond to media inquiries. Do not participate in interviews or other media responses. Hotel Parking • In the unlikely event that outsiders disrupt a scientific session or Self parking is available for $20 per day for Sunday night through other event, please follow the chairperson’s directions and avoid Thursday night stays. Parking for Friday and Saturday night stays becoming involved in the situation. is complimentary (8:00 pm Friday until midnight Sunday). Valet Parking is available for $24 per day. Prices are subject to change. Our first priority is safety. The best way to stay safe is to be aware of your surroundings and avoid situations where you feel Meals uncomfortable. A light breakfast is provided for registrants of the Special Interest Internet Access at the Annual Meeting Group meetings, held Sunday–Tuesday. A light lunch will be provided for the WEMS SIG on Tuesday. You are on your own for EMS appreciates how important it is for attendees to stay connected lunch and dinner each day with the exception of Monday’s Business to daily responsibilities in their home locations while attending the Meeting and Town Hall which includes a boxed lunch and Tuesday’s meeting. EMS registered meeting attendees will have access to the Banquet, which will include dinner and dancing. EMS Internet Café during registration hours. The Hyatt Regency Bellevue also offers complimentary Internet in Tully’s, located in the For meals on your own, there are several dining options in the hotel, lobby. Please note that this service is not available in the meeting within walking distance, or a short taxi ride away. More information rooms or the meeting room foyers. The Hyatt Regency Bellevue about Bellevue restaurants can be obtained from the concierge. offers a tiered price structure and multiday offers for guest room wired or wireless Internet in sleeping rooms. Conference Site Step into the sophisticated Hyatt Regency Bellevue on Seattle’s desirable Eastside. Rich woods, natural finishes and luxurious fabrics set the tone for an exceptional guest experience. The Hyatt Regency Bellevue is located just 20 minutes from the Seattle- Tacoma International Airport and nine miles east of downtown Seattle. Stroll through connecting sky bridges and discover more than 250 shops, 45 restaurants and lounges, and plenty of entertainment options harbored inside this chic urban streetscape. The Hyatt Regency Bellevue offers many choices for dining and entertainment, including Eques, featuring a daily breakfast buffet, Tully’s Coffee offering coffee and smoothies with fresh baked goods, Daniel’s Broiler, which boasts a USDA Prime Steakhouse and an impressive view of Seattle skyline, Koral Bar and Kitchen, a “new American Kitchen” in a relaxed environment, Joey Bellevue serving

12 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

Agenda AGENDA 11:30 AM Detection of PIGO-Deficient DT40 Cells Using Friday, September 7 Aerolysin Jun Nakamura, University of North Carolina at 4:00 PM–6:00 PM Grand Staircase Landing (2nd Floor) Chapel Hill REGISTRATION 11:50 AM Discussion Saturday, September 8

7:00 AM–6:00 PM Grand Ballroom Foyer 12:00 Noon–1:00 PM REGISTRATION Lunch on Your Own

7:00 AM–6:00 PM Green Room (3rd Floor) Speaker Ready Room Open

1:00 PM–5:00 PM Auditorium 8:00 AM–12:00 Noon Auditorium workshop 2 WORKSHOP 1 New Developments in Genetic Toxicology: New Developments in Genetic Toxicology Testing: Automated In Vitro Micronucleus Assay The Pig-a Assay Chairpersons: Steven M. Bryce, Litron Laboratories and 8 September, September 7–Saturday, Friday, Chairpersons: Stephen D. Dertinger, Litron Laboratories and Krista L. Dobo, Pfizer Global Research and Development Robert H. Heflich, National Center for Toxicological Research, US FDA Primary Sponsors: Pfizer Global Research and Development and WIL Research Contributing Sponsors: Bristol-Myers Squibb, Litron Laboratories, and Miltenyi Biotec Contributing Sponsor: Litron Laboratories Supported in Part by: BioReliance Corporation, Supported in Part by: BioReliance Corporation, Genetic Toxicology Association, and John J. Nicolette Genetic Toxicology Association, and John J. Nicolette 8:00 AM Introduction to Workshop Goals, Pig-a Assay 1:00 PM Introduction Terminology and Principles Krista L. Dobo, Pfizer Global Research and Robert H. Heflich, National Center for Toxicological Development Research, US FDA 1:10 PM Automation of the In Vitro Micronucleus Assay 8:15 AM Pig-a Mutations: From the Pathogenesis of PNH Using Flow Cytometry: An Overview to Assessing the Mutation Rate in Humans Steven M. Bryce, Litron Laboratories Lucio Luzzatto, Istituto Toscano Tumori 1:40 PM Automated In Vitro Micronucleus Assay in V79 9:00 AM Update on the International Rat Erythrocyte Cells Using Flow Cytometry: Screening and

Pig-a Trial Assessing Mode of Action Stephen D. Dertinger, Litron Laboratories John J. Nicolette, Abbott Laboratories 9:45 AM Integration Case Study: 28-Day MNU Study with 2:10 PM Increasing Genotoxicity Screening Capacity: Multiple Genetox Endpoints Assessment of Micronucleus Induction with Laura L. Custer, Bristol-Myers Squibb Aneugen/Clastogen Characterization by High Capacity Flow (HCF) 10:15 AM Break (Auditorium Foyer) Kim Luu, IntelliCyt Corporation 10:30 AM Pig-a, Micronucleus, and Comet Assay 2:40 PM Break (Auditorium Foyer) Combined in a Three-Day Rat Repeated Dose Study with DMBA 2:55 PM Automated In Vitro Micronucleus Assay for Bas-jan M. van der Leede, Janssen Research and Screening of Pharmaceuticals in TK6 Cells Using Development Flow Cytometry with 96-Well Plates Marlies De Boeck, Johnson & Johnson Pharma 11:00 AM How Well Does the Pig-a Assay Complement Research and Development Other In Vivo Genetox Assays in Detecting Rodent Carcinogens? 3:25 PM Automation of the In Vitro Primary Human Robert H. Heflich, National Center for Toxicological Lymphocyte Micronucleus Assay: Image Analysis Research, US FDA versus Flow Cytometry Azeddine Elhajouji, Novartis Pharma AG www.ems-us.org 13 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

4:05 PM Use of Image Analysis to Automate Mechanistic Investigations in the In Vitro MN Assay Maik J. Schuler, Pfizer Global Research and Development

Saturday, 8–Sunday, September 9 September 4:45 PM Discussion

1:00 PM–5:00 PM Grand Ballroom A workshop 3 Next Generation Technologies Chairpersons: Matthew A. Coleman, Lawrence Livermore National Laboratory, Dana Farber Cancer Institute and Paul Van Hummelen, Center for Cancer Genome Discovery Primary Sponsor: Illumina Contributing Sponsor: Agilent Technologies 1:00 PM Getting a Handle on Starting a NGS Study Matthew A. Coleman, Lawrence Livermore National Laboratory 1:10 PM Genomic Approaches towards Individualized Cancer Medicine Paul Van Hummelen, Center for Cancer Genome Discovery (CCGD), Dana Farber Cancer Institute 1:50 PM Genomic Studies for Clinical and Genomic Screens Jay A. Shendure, University of Washington Sunday, September 9 2:30 PM Break (Grand Ballroom Foyer) 2:45 PM Detecting New Human Mutations Using Parent- 7:00 AM–5:00 PM Grand Ballroom Foyer Child Trios: Characteristics and Role in Autism REGISTRATION Spectrum Disorders Brian J. O’Roak, University of Washington 3:20 PM Inverse PCR Screen of Leukemogenic 7:00 AM–5:00 PM Grand Ballroom E Fusiongenes and Analysis by Parallel Sequencing Speaker Ready Room Open Andrew T.M. Vaughan, University of California, Davis 7:00 AM–8:30 AM BREAKFAST MEETINGS 3:55 PM Sequencing the Transcriptome: Motivations, Workflows, Challenges, and Successes Breakfast Pickup Evergreen Ballroom Foyer Mick Correll, Center for Cancer Computational for Meetings Biology, Dana Farber Cancer Institute (Ticket Required—Breakfast Available at 6:45 AM)

4:30 PM Concluding Remarks Applied Genetic Toxicology Evergreen Ballroom A Special Interest Group 5:00 PM–7:00 PM Evergreen Ballroom E Leaders: Krista L. Dobo, Pfizer Global Research and Development and John J. Nicolette, Abbott Laboratories WELCOME RECEPTION AND Student and NEW INVESTIGATOR POSTER SESSION 7:00 AM Welcome Supported in part by: Genetic Toxicology Association 7:15 AM In Vivo Comet Assay: 2-Acetylaminofluorene Case Study Brian Burlinson, Huntingdon Life Sciences 7:45 AM Implementation of Updated Genotoxicity Guidance ICH S2(R1) Sheila M. Galloway, Merck Research Laboratories 8:00 AM ICH S2(R1): A Survey of Industry Practice To Be Presented by the SIG Chairs

14 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

DNA Repair and Evergreen Ballroom B 10:45 AM Break (Grand Ballroom Foyer) Mutagenic Mechanisms 11:00 AM S3 Recombomice and CometChip Technology Special Interest Group AGENDA Shed Light on Gene-Exposure Interactions That Leaders: Robert W. Sobol Jr., University of Pittsburgh Cancer Impact Genomic Stability Institute and Joann B. Sweasy, Yale University School of Medicine Bevin P. Engelward, Massachusetts Institute of 7:00 AM Bring Your Ideas to Bellevue: Discussion of Technology Topics for the 2013 EMS Annual Meeting 11:30 AM S4 The Interaction and Regulation of Polß, XRCC1, 7:45 AM General Discussion: DNA Repair Drug and PARP1 in Response to DNA Damage in Development and the Emergence of Synthetic Tumor Cells Lethality As a Research Tool for Pathway and Robert W. Sobol Jr., University of Pittsburgh Cancer Gene Discovery As Well As a Viable Clinical Institute Option for Cancer Therapeutics 12:00 Noon S5 Etiologic Involvement of Genome Damage in Heritable Mutation Evergreen Ballroom C Inflammation and Disease Sankar Mitra, University of Texas, Galveston September 9 Sunday, Special Interest Group Leaders: Francesco Marchetti, Health Canada, Christopher M. 9:45 AM–12:30 PM Grand Ballroom I Somers, University of Regina, and Carole L. Yauk, Heath Canada SYMPOSIUM 2 7:00 AM Welcome Emerging Approaches in Predictive Toxicology 7:05 AM Linking Endocrine and Genetic Effects in the Male Germline: Insights into Mechanisms Chairpersons: Patricia A. Escobar, Boehringer Ingelheim Patricia Hunt, Washington State University Pharmaceuticals and Sundaresan Venkatachalam, National Institutes of Health 7:35 AM Investigating Unstable Microsatellites in the Mouse Germline 9:45 AM S6 Overview of Predictive Toxicology: Needs and Marc A. Beal, University of Regina Challenges Nigel Green, Pfizer Global Research and 7:50 AM Update on IARC Panel to Designate Human Development Germ Cell Mutagens David M. DeMarini, US Environmental Protection 10:15 AM S7 Computerized Docking Studies to Predict Agency Noncovalent Binding of Chemicals to DNA: Recent Experience with Pharmaceuticals 8:05 AM Adverse Outcome Pathways for Germ Cells Ronald D. Snyder, RDS Consulting Services Carole L. Yauk, Health Canada 10:45 AM Break (Grand Ballroom Foyer) 8:15 AM Discussion 11:00 AM S8 Stem Cells in Predictive Toxicology Testing Ivan N. Rich, HemoGenix, Inc. 11:30 AM S9 3-D Models in Predictive Toxicology Testing Marilyn J. Aardema, BioReliance Corporation 12:00 Noon S10 Functional and Comparative Genomics: Systematic Screening for Genes and Pathways Involved in Human Susceptibility to Chemical Exposures

Luoping Zhang, University of California, Berkeley

9:45 AM–12:30 PM Grand Ballroom A 12:30 PM–1:45 PM Evergreen Ballroom A SYMPOSIUM 1 EMM Editorial Board Meeting Inflammation, Cancer, and Aging Chairpersons: Bevin P. Engelward and Leona D. Samson, 12:30 PM–2:00 PM Massachusetts Institute of Technology Lunch on Your Own 9:45 AM S1 DNA Damage Responses As Keystone Complexes Defending Against Cancer, Aging, and Reactive 12:30 PM–2:00 PM Evergreen Ballroom C Oxygen STUDENT AND NEW INVESTIGATOR Luncheon John A. Tainer, Scripps Research Institute (Advance Registration Required) 10:15 AM S2 DNA Repair Is Indispensable for Survival after Acute Inflammation in Mice Leona D. Samson, Massachusetts Institute of

Technology www.ems-us.org 15 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

2:00 PM–4:00 PM Grand Ballroom A 2:00 PM–4:00 PM Grand Ballroom I Platform Session 1 Platform Session 2 DNA Repair The Environment, Genes, and Public Health Chairpersons: Bennett Van Houten, University of Pittsburgh and Chairpersons: David M. DeMarini, US Environmental Protection Sunday, 9 September Sharon B. Cantor, UMASS Medical School Agency and Marina V. Bakhmutsky, Wayne State University Underlined author indicates presenter. Underlined author indicates presenter. 2:00 PM 1 Watching Nucleotide Excision Repair, One 2:00 PM 8 Health Consequences of the Interaction of Our Molecule at a Time Genome with Our Environment Bennett Van Houten, University of Pittsburgh David M. DeMarini, US Environmental Protection Agency 2:30 PM 2 Exploring the Reactivity Landscape for Abasic Sites in DNA 2:30 PM 9 Long-Term Depleted Uranium Exposure in Admiraal S, O’Brien P. University of Michigan Gulf War Veterans Does Not Cause Increased Medical School, Ann Arbor, MI, United States Chromosome Aberrations in Peripheral Blood Lymphocytes Measured by FISH Whole 2:45 PM 3 Mechanism of Release and Fate of Excised Chromosome Painting Oligonucleotides during Nucleotide Excision Bakhmutsky M1, McDiarmid M2,3, Squibb K2,3, Repair Oliver M2,3, Tucker J1. 1Wayne State University, 1,2 1 1 Kemp MG , Reardon JT , Lindsey-Boltz LA , Sancar Detroit, MI, United States, 2Department of Veterans 1 1 A . University of North Carolina, Chapel Hill, Affairs Medical Center, Baltimore, MD, United 2 NC, United States, Florida Institute of Technology, States, 3University of Maryland School of Medicine, Melbourne, FL, United States Baltimore, MD, United States 3:00 PM 4 Genomewide Assessment of DNA Repair and 2:45 PM 10 High Red Meat-Induced Promutagenic Adducts Transcriptional Recovery Following Exposure to in the Mouse and Human Colon and Their UV Light Inhibition by Resistant Starch 1,2 2 2 Andrade-Lima LC , Prasad J , Paulsen M , Veloso Winter J1, Nyskohus L1, Young GP1, Hu Y1, Conlon 2,3 1 4 2 A , Menck CFM , Wilson TE , Ljungman M . M2, Bird A2, Topping D2, LeLeu R1. 1Flinders 1 Biomedical Sciences Institute, University of São University of South Australia, Adelaide, South 2 Paulo, São Paulo, Brazil, Department of Radiation Australia, Australia, 2CSIRO Preventative Health Oncology, University of Michigan, Ann Arbor, Flagship, Adelaide, South Australia, Australia MI, United States, 3Department of Computational Medicine and Bioinformatics, University of 3:00 PM 11 Population Variation in Micronucleus Response Michigan, Ann Arbor, MI, United States, to Inhaled Benzene in Diversity Outbred 4Department of Pathology, University of Michigan, (J:DO) Mice Ann Arbor, MI, United States Hobbs CA1, Morgan DL3, Shepard K1, Price HC2, Kissling GE3, Shockley KR3, Recio L1, Witt KL3, 3:15 PM 5 XPG Promotes Homologous Recombination and French JE3. 1Integrated Laboratory Systems, Inc., Maintains Genome Stability Genetic and Molecular Toxicology Division, 1 2 1 1 Trego KS , Davalos AR , Groesser T , Wiese C , Research Triangle Park, NC, United States, 2Alion 1 1 1 1 Nelson MR , Tsai MS , Rydberg B , Pluth J , Sung Science and Technology, Life Sciences and Inhalation 3 1,2 1 1 P , Campisi J , Cooper PK . Lawrence Berkeley Toxicology Laboratory, Research Triangle Park, NC, National Laboratory, Berkeley, CA, United States, United States, 3NIEHS, Division of the National 2 The Buck Institute for Research on Aging, Novato, Toxicology Program, Research Triangle Park, NC, 3 CA, United States, Yale University, New Haven, CT, United States United States 3:15 PM 12 High-Throughput Detection of Variations in 3:30 PM 6 Identification of Novel Genes in BRCA1- Exposure-Induced DNA Damage and Repair in Regulated DNA Repair Pathways Human Cells Kotian S, Parvin J. The Ohio State University Ge J, Weingeist D, Wood D, Fessler J, Rowland E, Comprehensive Cancer Center, Columbus, OH, Engelward B. Massachusetts Institute of Technology, United States Cambridge, MA, United States 3:45 PM 7 Loss of MSH2 Suppresses DNA Repair Defects of 3:30 PM 13 Detection of Ultra Rare Mutations and DNA Fanconi Anemia Damage by Next Generation DNA Sequencing Cantor S, Peng M, Ucher A, Stavnezer J. UMASS Schmitt MW, Kennedy SR, Salk JJ. University of Medical School, Worcester, MA, United States Washington, Seattle, WA, United States 3:45 PM 14 Impact of TP53 Status on the Metabolic Activation of Environmental Polycyclic Aromatic Hydrocarbons Wohak LUE1,2, Seidel A3, Phillips DH2, Arlt VM2. 1Institute of Cancer Research, Sutton, Surrey, United Kingdom, 2King’s College London, London, United Kingdom, 3Biochemical Institute for Environmental Carcinogens, Grosshansdorf, Germany 16 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

4:00 PM–6:00 PM Evergreen Ballroom E P19 Interplay of Genes, Environment, and Tissue Physiology POSTER SESSION 1 AND EXHIBITS Controls Susceptibility to Large-Scale Sequence Rearrangements AGENDA Odd Numbered Posters Attended. Kiraly O, Roytman M, Engelward B. Department of Biological Presenting author is underlined. Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States P1 Systems Biology of Melanoma Kaufmann W, University of North Carolina, Chapel Hill, Chapel P21 Prolonged ATF2 Signaling Is a Potential Tool to Monitor Hill, NC, United States Oxidative Stress and Associated Cancer Susceptibility Sridharan DM1, Chan R2, Guo M2, Wilson WC1, Whalen MK1, P3 Telomere Shortening Delays DNA Damage Response in Pluth JM1. 1Lawrence Berkeley National Laboratory, Berkeley, CA, Human Cells United States, 2University of California, Berkeley, Berkeley, CA, Drissi R1, Wu J2, Bockhold C2, Dome J3. 1Division of Oncology, United States Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, United States, 2Department of Oncology, St. Jude Children’s P23 Roles of Mre11/CDK2 Interaction in the DNA Damage Research Hospital, Memphis, TN, United States, 3Division of Response Oncology, Children’s National Medical Center, Washington, DC, Festerling T, Buis J, Ferguson D. University of Michigan, September 9 Sunday, United States Ann Arbor, MI, United States P5 Characterization of Tumor-Associated Variants of MBD4 P25 Effect of Drinking Water Disinfection By-Products (DBPs) Sjolund AB, Sweasy JB. Yale University, New Haven, CT, United in Human Peripheral Blood Lymphocytes and Sperm States Ali A1, Kurzawa-Zegota M1, Najafzadeh M1, Gopalan RC1, Plewa M J2, Anderson D1. 1University of Bradford, Bradford, P7 Probing the Polβ/XRCC1 Interaction and Its Role in West Yorkshire, United Kingdom, 2University of Illinois, Urbana- Stability of DNA Polymerase ß, Base Excision Repair, and Champaign, IL, United States Cellular Response to DNA Damage Fang Q1,2, Lan L2, Svilar D1,2, Schamus S2, Wang X-H2, Sugrue P27 Taking Vitamin E or Not: γ-tocopherol Induces XPA K2, Goellner E1,2, Sobol R1,2. 1Department of Pharmacology & Overexpression and Promotes Nucleotide Excision Repair Chemical Biology, University of Pittsburgh School of Medicine, Li Z, Wang H, Campbell S, Guo H, Musich P, Zou Y. Department Pittsburgh, PA, United States, 2University of Pittsburgh Cancer of Biochemistry and Molecular Biology, East Tennessee State Institute, Hillman Cancer Center, Pittsburgh, PA, United States University, J.H. Quillen College of Medicine, Johnson City, TN, United States P9 The Nitrosated Bile Acid DNA Lesion, O6-carboxymethylguanine, Is a Substrate for Human P29 Association of TGFβ-1 Promoter Polymorphism with O6-alkylguanine DNA Alkyltransferase Bronchial Asthma in South Indian Population Senthong P1, Williams DM3, Wilkinson O3, Millington C3, Mundluru Hema P, Srinivas B, Sreedevi V, Priyanka P, Silaja V, Marriott A2, Watson AJ2, Kelly JR2, Latypov V2, McGown G2, Suresh G, Jyothy A. Institute of Genetics and Hospital for Genetic Thorncroft 2MR , Eyers CE4, Margison GP2, Povey AC1. 1Centre for Diseases,Osmania University, Begumpet, Hyderabad, A.P., India Occupational and Environmental Health, School of Community P31 Children Exposed to Metals Mixtures Demonstrate Based Medicine, Faculty of Medical & Human Sciences, University 2 Dysregulation of Infectious Disease Response of Manchester, Manchester, United Kingdom, Paterson Institute 1,2 1 1 1 3 3 Gruber JF , Patel RD , Rager JE , Sanders AP , Edwards SW , for Cancer Research, Manchester, United Kingdom, Department 3 1 1 of Chemistry, Centre for Chemical Biology Richard Roberts Gallagher JE , Fry RC . UNC Gillings School of Global Public Health, Department of Environmental Sciences and Engineering, Building, University of Sheffield, Sheffield, United Kingdom, 2 4Michael Barber Centre for Mass Spectrometry, Manchester Chapel Hill, NC, United States, UNC Gillings School of Global Public Health, Department of Epidemiology, Chapel Hill, NC, Interdisciplinary Biocentre, School of Chemistry, University of 3 Manchester, Manchester, United Kingdom United States, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency,

P11 Regulation of hMSH4 Protein Homeostasis by NC, United States Ubiquitination in Human Cells P33 Mapping of Transcription Start Sites and Enhancer Xu Y, Her C. Washington State University, Pullman, WA, United Elements Using BruUV-Seq States Veloso A, Paulsen M, Prasad J, Wilson T, Ljungman M. University P13 REV1 Plays Major Roles in PIG-O Mutations Caused by of Michigan, Ann Arbor, MI, United States MMS in DT40 Cells P35 Hydroxyurea Genotoxicity II: Reprotoxic and Nakamura J, Bultman S, Swenberg J, Tian X. University of North Transgenerational Effects on Drosophila Carolina at Chapel Hill, Chapel Hill, NC, United States Ramos P1, Muñoz A1, Hernandez BR1, Rivas H1, Muñoz JA2. 1Fac. P15 Chemotherapeutic Alkylation Treatment: Tumor versus Ciencias, UNAM, Mexico DF, Mexico, 2CCH-Sur, UNAM, Mexico Normal Cell Response DF, Mexico Gupte M1, Sharma V2, Williams K1. 1University of Toledo College P37 Mechanisms Underlying Inflammation-Associated of Medicine, Toledo, OH, United States, 2Bowling Green State Tumorigenesis: Progressive Epigenetic Changes in an University, Bowling Green, OH, United States Enterotoxigenic Bacteroides fragilis Murine Colitis Model P17 The Effect of Environmental Pollutants on DNA Repair DeStefano Shields CE1,2, Van Meerbeke SW2, Baylin SB2, Sears and Damage CL2, O’Hagan HM2, Casero RA2. 1The Johns Hopkins Bloomberg Sram R, Mrhalkova A, Uhlirova K, Spatova M, Rossner P. Institute School of Public Health, Baltimore, MD, United States, 2The Johns of Experimental Medicine AS CR, Prague, Czech Republic Hopkins University School of Medicine, Baltimore, MD, United

States www.ems-us.org 17 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

P39 Benzene Metabolites Enhance the Secretion of Transferrin P57 Toxicokinetics Are Essential for In Vivo/In Vitro Receptor in K562 Cells Extrapolation of B[a]P-Induced Carcinogenomic Daniels SI, Tu B, Chang C, Zhang L, Smith MT. UC Berkeley, Responses Berkeley, CA, United States van Kesteren PC1,2, Zwart PE1, Schaap MM1,4, Pronk TE1, van Herwijnen MH2, Godschalk RW3, Zeilmaker MJ5, Bokkers BG5,

Sunday, 9 September P41 The Harlequin Mouse Model of Premature Aging van Steeg H1,4, Luijten M1. 1Laboratory for Health Protection Displays Increased p53 Signalling, Evidence of Vascular Research, National Institute for Public Health and the Dysfunction and Inflammation in the Retina and Environment (RIVM), Bilthoven, Netherlands, 2Department of Cerebellum Toxicogenomics, Maastricht University, Maastricht, Netherlands, Laliberte AM, Mayers J, MacPherson TC, Edwards M, 3Department of Toxicology, Maastricht University, Maastricht, Kisilevsky I, Thwaites M, Hutnik CML, Hill KA. Western Netherlands, 4Department of Toxicogenetics, Leiden University University, London, ON, Canada Medical Center (LUMC), Leiden, Netherlands, 5Centre for P43 Evaluation of Repeated Dose Gastrointestinal Tract Substances and Integral Risk Assessment, National Institute Micronucleus Assay in Rats: Summary of Collaborative for Public Health and the Environment (RIVM), Bilthoven, Study by CSGMT/JEMS.MMS Netherlands Ohyama W2, Narumi K2, Fujiishi Y2, Hori H3, Matsumura S4, P59 Dramatically Increasing the Capacity of Genotoxicity Ikeda N4, Natsume M1, Tanaka J1, Takashima R5, Hamada Testing: Automating a Flow Cytometry-Based In Vitro S5, Asano N6, Morita T7, Kojima H7, Honma M7, Hayashi M1. Micronucleus Assay 1 2 Biosafety Research Center, Iwata, Shizuoka, Japan, Yakult Liu Z1, Bryce S2, Bemis J2, Luu YK1. 1IntelliCyt Corporation, 3 Honsha, Tokyo, Japan, Suntory Business Expert, Osaka, Japan, Albuquerque, NM, United States, 2Litron Laboratories, Rochester, 4 5 Kao Corporation, Tochigi, Japan, Mitsubishi Chemical Medience NY, United States Corporation, Ibaraki, Japan, 6Kinki University, Osaka, Japan, 7National Institute of Health Sciences, Tokyo, Japan P61 Characterization of DNA-Reactive and NonDNA-Reactive Pharmaceutical Drug Candidates by Gene Expression P45 Withdrawn Profiling P47 Spermicidal Activity of Heliopsis longipes S.F. Blake Heard PL1, Dickinson DA1, Aubrecht J1, Li H2, Fornace AJ2, Li (chilcuague) Root Extract Y1, Henstock P1, Enayetallah A1, Koza-Taylor PH1, Pelletier DJ1, Cariño-Cortés R1, Sánchez-Gutiérrez M1, Izquierdo-Vega JA1, Schuler M1. 1Pfizer, Groton, CT, United States,2 Georgetown Martínez-Loredo E1, Zúñiga-Pérez C1, Cilia-López GV2, Moreno- University, Washington, DC, United States 3 1 Martínez E . Instituto de Ciencias de la Salud, Universidad P63 Tissue Dynamics in PAH Carcinogenicity: Autonoma del Estado de Hidalgo, Pachuca de Soto, Hidalgo, A Toxicogenomics Perspective Mexico, 2Departamento de Toxicología Ambiental, Facultad de Labib S1,2, Guo C1,2, Williams A3, White PA1,2, Halappanavar SO1. Medicina, Universidad Autónoma de San Luis Potosí, San Luis 1 2 3 University of Ottawa, Ottawa, ON, Canada, Mechanistic Studies Potosí, S.L.P., Mexico, Unidad de Fisiología Molecular, Instituto 3 Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Division, Health Canada, Ottawa, ON, Canada, Population México DF, México, Mexico Studies Division, Health Canada, Ottawa, ON, Canada P65 Genotoxicity in Mice, Mus Norvegicus Albinus on Exposure P49 ToxML: A Common Exchange Standard for Raw and Summarized Genetic Toxicology Data and Data Analysis to Fluoride Aluminium and Their Combination Results Bhaskara Rao AV. Pondicherry University, Puducherry, India Benz RD1, Cross KP2, Bower DA2, Judson PN3, Barber CG4, Nath P67 Evaluation of Repeated Dose Liver Micronucleus Assay S5. 1US FDA/CDER/OPS/OTR/DDSR, Silver Spring, MD, United in Rats (II): Summary of Collaborative Study by CSGMT/ States, 2Leadscope, Inc., Columbus, OH, United States, 3Judson JEMS.MMS 4 Consulting Service, Harrogate, United Kingdom, Lhasa Limited, Hamada S1, Takashima R1, Shimada K2, Matsumoto K3, 5 Leeds, United Kingdom, PointCross Life Sciences, Foster City, CA, Kawakami S4, Tanaka J5, Matsumoto H6, Nakai T7, Imamura United States T8, Matsumura S9, Sanada H10, Inoue K11, Muto S12 , Hagio S13 , 14 15 16 17 P51 Spanish Black Radish (Raphanus Sativus L. Var niger) Hayashi A , Takayanagi T , Ogiwara Y , Maeda A , Narumi 18 1 13 18 1 Diet Enhances Clearance of DMBA and Diminishes Toxic K , Takasawa H , Ogawa I , Ohyama W , Wako Y , Kawasako 1 19 19 5 19 1 Effects on Bone Marrow Progenitor Cells K , Morita T , Kojima H , Hayashi M , Honma M . Mitsubishi 1 2 2 2 2 Chemical Medience Corporation, Kamisu, Ibaraki, Japan, N’jai A , Kemp M , Metzger B , Hanlon P , Robbins M , 2 3 Czuprynski C1, Barnes D2. 1University of Wisconsin, Madison, WI, Astellas Pharma, Osaka, Japan, Astellas Research Technologies, Osaka, Japan, 4 5 United States, 2Standard Process, Inc, Palmyra, WI, United States Asahi Kasei Pharma, Shizuoka, Japan, Biosafety Research Center, Shizuoka, Japan, 6Food and Drug Safety Center, P53 Novel Therapeutic Compounds Yel001 and Yel002 Mitigate Kanagawa, Japan, 7Hokko Chemical Industry, Kanagawa, Japan, Radiation-Induced Toxicity 8Ina Research, Nagano, Japan, 9Kao Corporation, Tochigi, Japan, Schiestl RT, Rivina Y. University of California, Los Angeles, CA, 10Kaken Pharmaceutical, Shizuoka, Japan, 11Maruho, Kyoto, United States Japan, 12Mitsubishi Tanabe Pharma, Chiba, Japan, 13Nissan Chemical Industries, Saitama, Japan, 14Shin Nippon Biomedical P55 Benzo[a]pyrene Induces Gene Mutations in Mouse Laboratories, Kagoshima, Japan, 15Suntory Business Expert, Spermatogonial Stem Cells Osaka, Japan, 16Taisho Pharmaceutical, Saitama, Japan, 17Toray Marchetti F, O’Brien JM, Gingerich J, Soper L, Douglas G, Yauk Industries, Kanagawa, Japan, 18Yakult Honsha, Tokyo, Japan, CL. Health Canada, Ottawa, ON, Canada 19National Institute of Health Sciences, Tokyo, Japan

18 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

P69 The JaCVAM Validation Study of the In Vivo Comet Assay: P87 Genotoxicity of Aristolochic Acids in F344 Rats Selection of Test Chemicals Bhalli J, Shaddock J, Pearce M, Ding W, Dobrovolsky V, Heflich

Morita T1, Uno Y2, Kojima H1,3, Hayashi M4, Tice R5, Corvi R6, R. Division of Genetic and Molecular Toxicology, National AGENDA Schectman L7. 1National Institute of Health Sciences, Tokyo, Center for Toxicological Research (NCTR), US Food and Drug Japan, 2Mitsubishi Tanabe Pharma, Chiba, Japan, 3Japanese Administration (FDA), Jefferson, AR, United States Center for the Validation of Alternative Methods, Tokyo, Japan, P89 Single and Combined Effects of Celecoxib and Bleomycin 4Biosafety Research Center, Shizuoka, Japan, 5National Institute of Environmental Health Sciences, Division of the National Schumacher B, Gothke S, Dudley R, Vaglenov A. University of Toxicology Program, Hillsborough, NC, United States, 6European Findlay, Findlay, OH, United States Union Reference Laboratory for Alternative Methods to Animal P91 The Role of p53 in Genotoxicity Testing in Human 7 Testing, Ispra, Italy, Innovative Toxicology Consulting, LLC, Lake Lymphoblastoid Cells Worth, FL, United States Bruesehafer K1, Manshian BB1, Rees BJ1, Doak SH1, Doherty AT2, P71 Evaluation of In Vivo Genotoxicity Induced by N-ethyl-N- O’Donovan MR2, Jenkins GJS1. 1Swansea University, Swansea, nitrosourea, benzo[a]pyrene and 4-nitroquinoline-1-oxide United Kingdom, 2AstraZeneca R&D, Chesire, United Kingdom by Pig-a and gpt Assays P93 Reevaluation of the Mouse Lymphoma Forward Mutation September 9 Sunday, 1 1 2 1 1 Horibata K , Ukai A , Kimoto T , Suzuki T , Kamoshita N , Assay (MLA) Results on 342 Chemicals from the US 1 1 1 1 Masumura K , Nohmi T , Honma M . National Institute of National Toxicology Program’s (NTP) Database 2 Health Sciences, Japan, Tokyo, Japan, TEIJIN Pharma Limited, Schisler M1, Gollapudi B1, McDaniel P2, Moore M2. 1The Dow Tokyo, Japan Chemical Company, Midland, MI, United States, 2National Center P73 Investigation of the Dose-Response Curve for Isopropyl for Toxicological Research, US Food and Drug Administration, Methanesulfonate Using the In Vivo Pig-a Mutation Assay Jefferson, AR, United States Coffing SL, Kenyon MO, Ackerman JI, Dobo KL. Pfizer Inc., P95 Withdrawn Groton, CT, United States P97 Lessons Learned from Technical Variations of the P75 Pig-a Mutation, Micronucleus and Comet Assay Acellular Comet Assay Combined in Three-Day Rat Repeated Dose Studies with Dewhurst N, Grizzle M, Vasquez M. Helix3 Inc., Morrisville, NC, 7,12-Dimethylbenz(a)anthracene United States van der Leede B-jM1, Van Doninck T1, Schuermans A1, De Vlieger K1, De Kooning D1, Dertinger SD2, Van Gompel J1. 1Janssen P99 Maximizing the Utility of the In Vivo TGR Muta™Mouse Research & Development, A Division of Janssen Pharmaceutica Assay via Simultaneous Assessment of Multiple N.V., Beerse, Belgium, 2Litron Laboratories, Rochester, NY, United Genotoxicity Endpoints States Long AS1,2, Lemieux CL2, Gingerich JD2, Ritz CE2, Dertinger SD3, White PA1,2. 1University of Ottawa, Ottawa, ON, Canada, 2Health P77 Investigation of the Prevalence of Positive Results from Canada, Ottawa, ON, Canada, 3Litron Laboratories, Rochester, the GADD45a-GFP “GreenScreen HC” Genotoxicity Assay NY, United States Amongst NSAIDs, Apoptogens and HDAC Inhibitors Walmsley RM1,2, Allsup J2, Scott H2, Topham C1, Johnson D3, P101 Mixtures of Arsenite and Methyl Methanesulfonate Induce Billinton N2. 1University of Manchester, Manchester, United Synergistic Changes in Bcl2, p53, and pH2AX Kingdom, 2Gentronix Ltd, Manchester, United Kingdom, 3Harper Balbuena P1, Clewell R1, Sun B1, Ross S1, Gentry R2, Jager JW3, Adams University College, Shropshire, United Kingdom Clewell H1. 1The Hamner Institutes for Health Sciences, Research Triangle Park, NC, United States, 2Environ International, Monroe, P79 Genotoxic Effects of Novel Synthetic Triazole Containing LA, United States, 3University of New Mexico, Albuquerque, NM, Estradiol Analogs United States Gothke S, Reiff L, Schneider R, Dudley R, Vaglenov A. University of Findlay, Findlay, OH, United States P103 In Vitro Micronucleus Evaluation of Five Ames Negative Rodent Carcinogens

P81 In Vitro Assessment of Benzo-a-Pyrene-Induced Roberts DJ, Nicotra S, Mack M. Bristol-Myers Squibb, New Genotoxicity and Point Mutations in the Human HPRT Brunswick, NJ, United States Gene Shah U-K1, Seager AL1, Doak SH1, Johnson GE1, Carmichael P105 Qualification of a 96-Well High-Throughput In Vitro PL2, Scott SJ2, Scott AD2, Jenkins GJS1. 1Institute of Life Sciences, Micronucleus Assay in CHO Cells Using Flow Cytometry Swansea University, Wales, United Kingdom, 2Safety and Stankowski Jr. LF, Lawlor TE, Aardema MJ. BioReliance Environmental Assurance Centre, Unilever, Bedford, United Corporation, Rockville, MD, United States Kingdom P107 Response Criteria Reduces Impact of TK6 In Vitro P83 Mutagenic Potency of Four Chemicals Contained in Micronucleus Scoring Variability Cigarette Smoke Munzner JB, Homiski ML, Dobo KL, Sobol Z. Pfizer Inc, Groton, Guo X1, Lin H1, Dial S1, Richter P2, Moore M1, Mei N1. 1National CT, United States Center for Toxicological Research, US FDA, Jefferson, AR, United P109 Mutational Studies of Arrhythmogenic Right Ventricular States, 2Center for Tobacco Products, Rockville, MD, United States Cardiomyopathy in Indian Cohort P85 Comparison of Comet, Micronucleus, and Pig-a Mutation Nallari P. Osmania University, Hyderabad, Andhra Pradesh, India Endpoints in a 28-Day Oral Repeat Dose Study in Rats with MNU Roberts DJ, Nicotra S, Mack M, Gleason C, Custer LL. Bristol- Myers Squibb, New Brunswick, NJ, United States www.ems-us.org 19 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

P111 Mutagenicity of Diesel and Soy Biodiesel Exhaust Particles P125 The Nuclear Form of the Mitochondrial RNA Polymerase Mutlu E1,2, Warren SH2, Matthews PP2, King CJ2, Preston B3, Hays Responds to UV DNA Damage and Mitochondrial MD2, Nash DG2,4, Linak WP2, Gilmour MI2, DeMarini DM2. Stress and Is Required for Leukemia Cell Survival after 1University of North Carolina, Chapel Hill, NC, United States, 2US Mitochondrial Stress Environmental Agency, Research Triangle Park, NC, United States, Bralha F1, Schimmer A2,1, Laposa R1. 1University of Toronto,

Sunday, 9–Monday, September 10 September 3ARCADIS G&M, Inc., Durham, NC, United States, 4ORISE, Oak Toronto, ON, Canada, 2Princess Margaret Hospital, Toronto, ON, Ridge, TN, United States Canada P113 High-Resolution Detection of Cancer-Specific Mutations P127 Transcriptional Mutagenesis from 8-oxoguanine or in Patient Blood 8,5’-cyclo-2’-deoxyadenosine in Adult Neural Stem Cells Bertout JA1, Ericson NG1, Morrissey C2, Tella H1, Taylor SD1, Olanbiwonnu T1, Brooks P2, Tamblyn L1, Laposa R1. 1University Brown LG2, Noteboom J2, True LD2, Vessella RL2, Robins HS1, of Toronto, Toronto, ON, Canada, 2Laboratory of Neurogenetics, Drescher CW1,4, Bielas JH1,2. 1Fred Hutchinson Cancer Research National Institute on Alcohol Abuse and Alcoholism, Bethesda, Center, Seattle, WA, United States, 2University of Washington, MD, United States Seattle, WA, United States, 3Department of Veterans Affairs Medical Center, Seattle, WA, United States, 4Pacific Gynecology P129 The Effect of Ribonucleotide Incorporation on DNA Specialists, Seattle, WA, United States Polymerase Eta Fidelity Beardslee RA, McCulloch SD. North Carolina State University, P115 Detection of Genotoxic and Nongenotoxic Carcinogens in Raleigh, NC, United States Xpc-/-p53+/- Mice Melis J1,2, Speksnijder E1, Schaap M1,2, Kuiper R3, Salvatori D4, P131 Beyond Single Gene Mutation Targets: An Array-Based, Maas S4, Robinson J3, Verhoef A2, van Benthem J2, Luijten M2, Genomewide Approach to the Study of Somatic Mosaicism van Steeg H2,1. 1Leiden University Medical Center, Department Eitutis ST, Wishart AE, Hill KA. Western University, London, ON, of Toxicogenetics, Leiden, Netherlands, 2Laboratory for Health Canada Protection Research, National Institute for Public Health and the P133 Benzo[a]pyrene Mutagenicity on Mitochondrial DNA Environment, Bilthoven, Netherlands, 3Dutch Molecular Pathology Ericson N1, Lemieux C2, Marchetti F2, White P2, Bielas J1,3. Center, Department of Pathobiology, Faculty of Veterinary 1Fred Hutchinson Cancer Research Center, Seattle, WA, United Medicine, Utrecht University, Utrecht, Netherlands, 4Leiden States, 2Health Canada, Ottowa, ON, Canada, 3University of University Medical Center, Central Animal Facility, Leiden, Washington, Seattle, WA, United States Netherlands P135 Deoxyribonucleotide Pool Imbalances and Mitochondrial P117 Induction of cII and K-Ras Mutation in Lung DNA of Big Mutagenesis Blue Mice Exposed to Ethylene Oxide by Inhalation Kuong KJ, Loeb LA. University of Washington, Seattle, WA, Parsons BL1, Manjanatha MG1, Myers MB1, McKim KL1, Wang Y 1, Gollapudi BB2, Moore N3, Haber LT4, Moore MM1. United States 1National Center For Toxicological Research, US Food and Drug Administration, Jefferson, AR, United States,2 Toxicology & Environmental Research and Consulting, Dow Chemical 6:00 PM–7:00 PM COMMITTEE MEETINGS 3 Company, Midland, MI, United States, Dow Europe GmbH, 2013 Program Committee Larch Horgen, Switzerland, 4TERA NA, Cincinnati, OH, United States (First Meeting) P119 Investigating Transplacental Carcinogenesis of Environmental PAH Mixtures in a Mouse Model Awards and Honors Committee Juniper Larkin A, Siddens LK, Do BN, Dunn KC, Madeen EP, Krueger SK, Baird WM, Williams DE. Oregon State University, Corvallis, OR, Public Relations and Madrona United States Communications Committee

P121 The Syrian Hamster Embryo (SHE) Cell Transformation Publication Policy Committee Laurel Assay: Results with Three Dietary Ingredients— Menadione, Curcumin, and Quercetin Hydrate 1 1 1 1 2 Pant K , Bruce SW , Sly JE , Klug-Laforce M , Scott AD , Aardema 8:00 PM–10:00 PM Lucky Strike Lanes MJ1. 1BioReliance, Rockville, MD, United States, 2Unilever, Bedfordshire, United Kingdom The EMS Bowling Challenge P123 Classification of Test Agents Based on Mechanism of Supported in Part by: BioReliance Corporation Action in Syrian Hamster Embryo (SHE) Cells Using ATR- FTIR Spectroscopy with Computational Analysis Ahmadzai AA1, Trevisan J1, Pang W1, Riding M1, Pant K3, Carmichael PL2, Scott AD2, Martin FL1. 1Lancaster University, Monday, September 10 Centre for Biophotonics, Lancaster, United Kingdom, 2Unilever, Safety & Environmental Assurance Centre, Bedford, United 7:00 AM–5:00 PM Grand Ballroom Foyer Kingdom, 3BioReliance Corporation, Rockville, MD, United States REGISTRATION

7:00 AM–5:00 PM Grand Ballroom E Speaker Ready Room Open

20 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

7:00 AM–8:30 AM BREAKFAST MEETINGS New Technologies Evergreen Ballroom C Special Interest Group

Breakfast Pickup Evergreen Ballroom Foyer Leader: Sundar Venkatachalam, National Institutes of Health AGENDA for Meetings (Ticket Required—Breakfast Available at 6:45 AM) 7:00 AM Welcome 7:15 AM Induced Pluripotent Stem Cells Epigenetics Evergreen Ballroom A Ivan N. Rich, HemoGenix Inc. Special Interest Group Leaders: Janet E. Baulch, University of Maryland, Baltimore, 7:45 AM Ὁmics Analysis of Archived Tissue Samples: Dana C. Dolinoy, University of Michigan, and Recent Advances Daneida Lizarraga Lopez, Maastricht University Leslie Recio, ILS, Inc. 7:00 AM Welcome and Introductions 8:15 AM Election of Co-Chair 7:15 AM P37 Mechanisms Underlying Inflammation- Associated Tumorigenesis: Progressive 8:30 AM–6:00 PM Evergreen Ballroom E Epigenetic Changes in an Enterotoxigenic Bacteroides fragilis Murine Colitis Mode POSTERS AND EXHIBITS OPEN September 10 Monday, Christina DeStefano Shields, The Johns Hopkins Bloomberg School of Public Health 7:40 AM P32 A Historical Overview of Cell-Based Assays for Detecting Epigenetic Effects Catherine B. Klein, New York University School of Medicine 8:05 AM Banning Bisphenol A in the United States and Canada: A Missed Opportunity to Protect the Fetus Mitchell Turker, Oregon Health & Science 9:45 AM–12:30 PM Grand Ballroom A University SYMPOSIUM 3 Molecular Epidemiology Evergreen Ballroom B Next Generation Environmental Epigenetics Special Interest Group Leaders: Miriam C. Poirier, National Cancer Institute and Chairpersons: Dana C. Dolinoy, The University of Michigan and Radim J. Sram, Institute of Experimental Medicine Heather M. O’Hagan, The Johns Hopkins University School of Medicine 7:00 AM Welcome 9:45 AM S11 Oxidative Damage-Induced Epigenetic Changes 7:15 AM 14 Impact of TP53 Status on the Metabolic Heather M. O’Hagan, The Johns Hopkins University Activation of Environmental Polycyclic Aromatic School of Medicine Hydrocarbons Volker M. Arlt, King’s College London 10:15 AM S12 Low-Dose Ionizing Radiation Induced Epigenetic Adaptations and Disease Risk 7:30 AM P57 Toxikokinetics are Essential for In Vivo/In Vitro Randy L. Jirtle, Duke University Medical Center Extrapolation of B[a]P-Induced Carcinogenomic Responses 10:45 AM Break (Grand Ballroom Foyer) Mirjam Luijten, National Institute for Public Health 11:00 AM S13 Environmentally-Induced Changes in the

and the Environment (RIVM) Epigenome 7:45 AM P63 Tissue Dynamics in PAH Carcinogenicity: A Dana C. Dolinoy, The University of Michigan Toxicogenomics Perspective 11:30 AM S14 Chromatin Responses to Double-Strand Breaks Sarah Labib, University of Ottawa Needed to Maintain Genomic Integrity: A Focus 8:00 AM 12 High-Throughput Detection of Variations in on Breast Cancer Exposure-Induced DNA Damage and Repair in Roger A. Greenberg, University of Pennsylvania Human Cells 12:00 Noon S15 Epigenetic Epidemiology and Environmental Jing Ge, Massachusetts Institute of Technology Health 8:15 AM Discussion Andrea Baccarelli, Harvard School of Public Health www.ems-us.org 21 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

9:45 AM–12:30 PM Grand Ballroom I 2:45 PM 17 Directed Histone Binding Damaged DNA in Cell SYMPOSIUM 4 Nuclear Extracts Hinz J, Duan M, Smerdon M. Washington State Next Generation Risk Assessment and Regulatory University, Pullman, WA, United States Policies

Monday, 10 September 3:00 PM 18 Effects of Age, Sex, and Prenatal Exposure on Chairpersons: Nagu Keshava, US Environmental Protection Agency, DNA Methylation in a Mexican-American Birth Mugimane G. Manjanatha and Suzanne M. Morris, National Cohort Center for Toxicological Research, US FDA Yousefi ,P Aguilar R, Quach H, Karen H, Volberg V, Bradman A, Eskenazi B, Holland N. CERCH, SPH, Supported in Part By: ILSI-HESI IVGT Committee University of California, Berkeley, CA, United States 9:45 AM S16 Strategies in the Application of Genetic Toxicity 3:15 PM 19 Novel Functions of Ataxia Telangtiectasia and Data to Risk Assessment Rad3-Related (ATR) in the Cytoplasm B. Bhaskar Gollapudi, The Dow Chemical Li Z1, Musich P1, Shkriabai N2, Kvaratskhelia M2, Company Zou Y1. 1Department of Biochemistry and Molecular 10:15 AM S17 Integration of New Technologies into Risk Biology, East Tennessee State University, J.H Quillen Assessment College of Medicine, Johnson City, TN, United States, 2Center for Retrovirus Research and Comprehensive Vincent J. Cogliano, US Environmental Protection Cancer Center, College of Pharmacy, The Ohio State Agency University, Columbus, OH, United States 10:45 AM Break (Grand Ballroom Foyer) 3:30 PM 20 Sex- and Diet-Dependent Epigenetic 11:00 AM S18 Ὁmic Biomarkers Downregulation of DNA Glycosylase Mbd4 Donna L. Mendrick, US Food and Drug in Mice Exposed to Inorganic Arsenic May Administration Contribute to Genomic Instability DeSimone MC, Bohn A, Threadgill DW. North 11:30 AM S19 Next Generation Risk Assessment and Carolina State University, Raleigh, NC, United States Regulatory Policies: Risk Characterization and Communication 3:45 PM 21 No Apparent Transgenerational Genetic J. David Miller, Carleton University Instability in the F1 or F2 Offspring of Male MutaTMMice Exposed to N-ethylnitrosourea 12:00 Noon S20 Next Generation Ethics and Health Policy O’Brien JM, Williams A, Douglas GR, Marchetti F, Mark A. Rothstein, University of Louisville Yauk CL. Health Canada, Ottawa, ON, Canada

12:30 PM–12:45 PM Grand Ballroom Foyer 2:00 PM–4:00 PM Grand Ballroom I Boxed Lunch Pickup Platform Session 4 (Ticket Required—For the EMS Business Meeting and Town Hall) Genotoxicity and Toxicogenomic Analyses of Environmental Contaminants 12:45 PM–2:00 PM Grand Ballroom I Chairpersons: Michael J. Plewa, University of Illinois at Urbana and Matias S. Attene Ramos, NIH Chemical Genomics Center Business Meeting and Town Hall (Boxed Lunches Available) Underlined author indicates presenter. 2:00 PM 22 Biological and Molecular Mechanisms for Drinking Water Disinfection Byproduct (DBPs) 2:00 PM–4:00 PM Grand Ballroom A Genotoxicity Platform Session 3 Michael J. Plewa, University of Illinois at Urbana Epigenetics and the DNA Damage Response 2:30 PM 23 Toxicity Assessment of Environmental Chemicals Chairpersons: William Kaufmann, University of North Carolina and Using a Quantitative High-Throughput Ana Luisa Kadekaro, University of Cincinnati Screening Approach Attene-Ramos M1, Huang R1, Sakamuru S1, Witt K2, Underlined author indicates presenter. Tice R2, Austin C1, Xia M1. 1NIH Chemical Genomics Center, NCATS, Bethesda, MD, United States, 2:00 PM 17 Epigenetics and the DNA Damage Response 2Division National Toxicology Program (DNTP), William Kaufmann, University of North Carolina NIEHS, Research Triangle Park, NC, United States 2:30 PM 16 Role of the Paracrine Factor Alpha-Melanocortin 2:45 PM 24 Development of a microRNA Assay to Evaluate and Its Receptor in Modulating the UVR- Genotoxicity of Agents Induced DNA Damage Chen T, Yan J, Chen X. Division of Genetic Kadekaro AL1, Chen J1,2, Chen S1, Yang J1, Swope V1, and Molecular Toxicology, National Center for Kavanagh-Starner R1, Jameson J1, Abdel-Malek Z1. Toxicological Research, US FDA, Jefferson, AR, 1University of Cincinnati, Cincinnati, OH, United United States States, 2Second Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu Province, China 22 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

3:00 PM 25 Cellular Stress Response to Engineered P10 Effect of InsP6 on DNA-PK-Dependent Phosphorylation Nanoparticles: Effect of Size, Surface Coating, Hanakahi L. University of Illinois, Chicago, IL, United States

and Cellular Uptake AGENDA Prasad RY1, McGee JK2, Killius MG1, Ackerman P12 Transcription Arrest at DNA Slipouts, Guanine-Rich DNA D2, Blackman CF2, DeMarini DM2, Simmons Sequences and Strand Breaks In Vitro: Implications for SO2. 1Student Services Contractor, US EPA, Genetic Instability and Human Disease Research Triangle Park, NC, United States, 2US Belotserkovskii BP, Neil AJ, Salinas-Rios V, Hanawalt PC. Stanford Environmental Protection Agency, Research Triangle University, Stanford, CA, United States Park, NC, United States P14 Interplay between Helicobacter pylori Infection and Host 3:15 PM 26 In Vivo Biomarkers for Genotoxic and Gastric Cancer-Associated Variant of DNA Polymerase Nongenotoxic Carcinogen Identification Beta Melis J1,2, Derks K3, Wackers P4, Schaap M2,1, Zwart Kidane MD, Sweasy J. Yale School of Medicine, New Haven, CT, E2, Jonker M4, Breit T4, Pothof J3, van Steeg H2,1, United States 2 1 Luijten M . Leiden University Medical Center, P16 Analyzing the Dynamic Localization of Base Excision Department of Toxicogenetics, Leiden, Netherlands, Repair by Quantitative Subcellular Compartmentalization 2Laboratory for Health Protection Research, National Analysis (Q-SCAN) September 10 Monday, Institute for Public Health and the Environment, Bauer N1, Corbett A1,2, Doetsch P1,2. 1Emory University School of Bilthoven, Netherlands, 3Erasmus University Medicine, Atlanta, GA, United States, 2Winship Cancer Institute, Medical Center, CGC Department of Genetics, Atlanta, GA, United States Rotterdam, Netherlands, 4University of Amsterdam (UvA), MicroArray Department & Integrative P18 Withdrawn Bioinformatics Unit, Amsterdam, Netherlands P20 Oligonucleotide Probe Retrieval Assay (OPRA): A 3:30 PM 27 High Information Content Flow Cytometric Versatile Methodology for Evaluating DNA Repair In Vitro Micronucleus Assay Mitigates the Capacity and Replication Fidelity In Vivo Influence of Apoptogenic Activity Shen JC1, Fox EJ1, Loeb LA1,2. 1Department of Pathology, Bryce S, Avlasevich S, Bemis J, Dertinger SD. Litron University of Washington, Seattle, WA, United States, Laboratories, Rochester, NY, United States 2Department of Biochemistry, University of Washington, Seattle, WA, United States 3:45 PM 28 Dose-Response of Mutagenicity Induced by Alkylating Genotoxins in Rat Peripheral Blood P22 Efficacy of Commonly Used Disinfectants for Inactivation Zeller A1, Tang L1, Guérard M1, Dertinger SD2, of Human Noroviruses and Its Surrogates Singer T1, Gocke E1. 1F. Hoffmann-La Roche, Ltd., Young J, Katz D. Duke University, Durham, NC, United States Basel, Switzerland, 2Litron Laboratories, Rochester, P24 Withdrawn NY, United States P26 Salmonella Mutagenicity of Canadian Cigarettes with Novel Design Features 4:00 PM–6:00 PM Evergreen Ballroom E Mladjenovic N1, Maertens RM2, Siu M1, Soo EC1, White 2 1 POSTER SESSION 2 AND EXHIBITS PA . Tobacco Research Division, Office of Research and Surveillance, Controlled Substances and Tobacco Directorate, Even Numbered Posters Attended. Health Canada, Ottawa, ON, Canada, 2Mechanistic Studies Presenting author is underlined. Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, Health P2 The Werner Syndrome Exonuclease Facilitates DNA Canada, Ottawa, ON, Canada Degradation and High Fidelity DNA Polymerization by Human DNA Polymerase δ P28 Fluoride-Induced Alterations in the Immune System of Mice Pups, Mus Norvegicus Albinus

Kamath-Loeb AS, Shen JC, Schmitt MW, Loeb LA. University of Washington, Seattle, WA, United States Bhaskara Rao AV. Pondicherry University, Puducherry, India. P4 Withdrawn P30 Withdrawn P6 Comet-Fish to Sensitively Assess Global and P32 A Historical Overview of Cell-Based Assays for Detecting Transcription-Coupled Repair of DNA Lesions Epigenetic Effects Guo J, Spivak G, Hanawalt P. Stanford University, Stanford, CA, Klein C. NYU School of Medicine, Tuxedo, NY, United States United States P34 Surrogate Endpoints Reveal Radiation Quality-Dependent P8 Differential Utilization of Importin -α4 and Importin Differences in Mammary Cancer Risk -α7 for UV-Induced Nuclear Import of Xeroderma Sridharan D1, Wilson W1, Whalen M1, Chappell L2, Cucinotta F2, Pigmentosum Group A Pluth J1. 1Lawrence Berkeley National Lab, Berkeley, CA, United 2 Li Z, Musich P, Cartwright B, Zou Y. Department of Biochemistry States, NASA, Lyndon B. Johnson Space Center, Houston, TX, and Molecular Biology, East Tennessee State University, J.H. United States Quillen College of Medicine, Johnson City, TN, United States P36 Development of a GLP 3D EpiDerm™ Reconstructed Human Skin Micronucleus Assay: A Promising Animals- Alternative Model Roy S, Szkudlinska A, Wang K, Shi J, Hickman S, Madraymootoo W, Aardema M. BioReliance, Rockville, MD, United States www.ems-us.org 23 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

P38 Disruption of Adrenal Steroidogenic Pathways and Steroid P56 Hydroxyurea Genotoxicity I: Effects on Germinal and Hormone Secretion in Human Adrenocortical H295R Somatic Cells of Drosophila Cells by Lower Chlorinated PCBs, Their Metabolites and Ramos P1, Muñoz A1, Hernandez BR1, Rivas H1, Muñoz JA2. Commercial Halogenated Biphenyl Mixtures 1Fac. Ciencias, UNAM, Mexico, DF, Mexico, 2CCH-Sur, UNAM, Flor S, Robertson L, Ludewig G. University of Iowa, Iowa City, IA, Mexico, DF, Mexico

Monday, 10 September United States P58 Quercetin and Curcumin Induce Oxidative DNA Damage P40 Reduction of Misleading “False” Positive Results in and p53 Pathways in Human Fibrosarcoma HT1080 Cells Mammalian Cell Genotoxicity Assays III: Sensitivity of Sun B1, White A2, Scott A2, Dent M2, Andersen M1, Clewell R1. p53 Functional Human Cells 1The Hamner Institute for Health Sciences, Durham, NC, United Fowler P2, Smith K1, Jeffrey 1L , Young J1, Fautz R5, Hewitt N6. States, 2Safety & Environmental Assurance Centre, Unilever, 1Covance Labs Ltd, Harrogate, United Kingdom, 2Unilever, Bedfordshire, United Kingdom Sharnbrook, United Kingdom, 3Procter & Gamble, Cincinnati, OH, United States, 4Kirkland Consulting, Tadcaster, United P60 Use of BrU-Seq and BrUChase-Seq to Study Cadmium- Kingdom, 5KPSS, Darmstadt, Germany, 6Hewitt Scientific Induced Changes in the Transcriptome and RNA Services, Erzhausen, Germany Stabilome in Human Fibroblasts Bedi K, Paulsen M, Lu B, Veloso A, Prasad J, Wilson T, Ljungman P42 A Japanese Collaborative Study on Rat Pig-a Assay: Report M. University of Michigan, Ann Arbor, MI, United States on Interlaboratory Difference in Pig-a Assay Using RBCs and RETs P62 Mode-of-Action of Silica Nanoparticle Genotoxicity: Kimoto T1, Horibata K2, Muto S3, Sanada H4, Okamoto M4, Pathway Analysis in Support of Secondary Mechanisms 1 1 1,2 1 1 1 Hashimoto K5, Itoh S5, Uno Y3, Honma M5. 1Teijin Pharma Sullivan A , Downs T , Crosby M , Shan Y , Pfuhler S . Procter 2 Limited, Tokyo, Japan, 2National Institute of Health Sciences, & Gamble Co, Cincinnati, OH, United States, AstraZeneca Tokyo, Japan, 3Mitsubishi Tanabe Pharma Corporation, Chiba, Pharmaceuticals, Waltham, MA, United States 4 Japan, Kaken Pharmaceutical Co., LTD., Shizuoka, Japan, P64 5 A Functional Genomics Approach in Yeast: The Role of Daiichi Sankyo Co., LTD, Shizuoka, Japan DNA Repair in Trichloroethylene Toxicity P44 Toxicity Testing in the 21st Century (TT21C): A Case De La Rosa V, Asfaha J, Vulpe C. University of California, Study Using DNA Damage Repair Pathways to Deliver Berkeley, Berkeley, CA, United States Safety Decisions for Consumer Products P66 1 1 1 2 1 The New Generation of the DEL Assay Detecting Scott A , Adeleye Y , Carmichael P , Clewell R , Davies M , Dent “Nonmutagenic” Carcinogens M1, Malcomber S1, White A1, Zhang Q2, Andersen M2. 1Unilever Parfenova L, Rivina Y, Kutepova V, Nguaen D, Schiestl R. Safety & Environmental Assurance Centre, Bedford, United University of California, Los Angeles, CA, United States Kingdom, 2The Hamner Institutes for Health Sciences, Durham, NC, United States P68 Mutagenicity and DNA Adduct Formation by Aristolochic Acid in the Spleen of Big Blue Rats P46 Identifying Structure-Activity Cliffs in a Salmonella QSAR Model for Predicting the Potential Mutagenicity of Mei N, McDaniel LP, Guo XQ, Chen T. National Center for Genotoxic Drug Impurities and Other Organic Molecules Toxicological Research, Jefferson, AR, United States Cross KP1, Benz RD2, Stavitskaya L2, Kruhlak NL2. 1Leadscope, P70 Unstable Simple Tandem Repeats in the Germline of Mice: Inc., Columbus, OH, United States, 2US FDA/CDER/OPS/OTR/ No Evidence for Mutation Induction Following Gamma DDSR, Silver Spring, MD, United States Radiation Exposure Beal MA1, Glenn TC2, Lance SL3, Somers CM1. 1University P48 Miniaturized Bacterial Mutagenicity Assays Designed to 2 Use Less Test Agent of Regina, Regina, SK, Canada, University of Georgia, Environmental Health Science, Athens, GA, United States, Elespuru R, Rajani A, Butler K. US FDA/CDRH, Silver Spring, 3University of Georgia, Savannah River Ecology Lab, Aiken, SC, MD, United States United States P50 Cyp1b1 Mediates Effects of Both AHP s and Endogenous P72 Use of Flow Cytometry to Distinguish Aneugenicity from Chemicals on Hematopoietic Progenitors in Bone Marrow 1,2 1 1 1 3 Clastogenicity in the In Vitro Micronucleus Assay with Njai A , Larsen M , Tong T , Bushkofsky J , Alexander D , Chinese Hamster V79 Cells Czuprynski C1, Jefcoate C1. 1University of Wisconsin, Madison, Murray J, Sonders P, Nicolette J. Abbott Laboratories, Abbott Park, WI, United States, 2Procter & Gamble, Cincinnati, OH, United IL, United States States, 3University of California, Santa Cruz, CA, United States P74 Micronucleus Assay and Comet Assay Using a Human P52 Chitosan That Dissolves in Hard Water Represses Hepatocyte Chimeric Mouse (PXB-mouse®) Mutagenicity Tanaka J1, Fukumuro M1, Shoji M1, Nakajima M1, Hayashi M1, Yoshikawa K 1, Shankel DM2. 1Kinki University, Nara, Japan, Ishida Y2, Kakuni M2, Tateno C2. 1Public Interest Incorporated 2University of Kansas, Lawrence, KS, United States Foundation Biosafety Research Center, Shizuoka, Japan, P54 Inflammation Underlies Neurodegeneration in Harlequin 2PhoenixBio Co., Ltd., Hiroshima, Japan Mice but Is Not a Key Target of Hormetic Doses of P76 Analysis of In Vivo Pig-a Gene Mutation and Phenobarbital Chromosomal Damage Potential of 13 Reference Prtenjaca A, Hill KA. The University of Western Ontario, London, Compounds ON, Canada Phonethepswath S1, Avlasevich S1, Torous D1, Mereness J1, Bryce S1, Bemis J1, MacGregor J2, Dertinger S1. 1Litron Laboratories, Rochester, NY, United States, 2Toxicology Consulting Services, Arnold, MD, United States 24 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

P78 Evaluation of p53 Genotype on Gene Expression and P96 2-Nitroethanol, but Not Related Nitroalcohols, Is Weakly O6-ethylguanine Levels in the Cerebrum from Male Mutagenic in Bacteria and Negative for Chromosomal and

C57BL/6 Mice Exposed to ENU DNA Damage In Vivo AGENDA Petibone D1, Kulkarni R1, Chang C-W2, Chen J2, Churchwell Swartz CD1, Hobbs CA1, Lentz C1, Garibaldi P1, Green A1, Recio M3, Morris S1. 1Division of Genetic and Molecular Toxicology, L1, Witt KL2. 1Integrated Laboratory Systems, Research Triangle Jefferson, AR, United States,2 Division of Personalized Medicine Park, NC, United States, 2National Institute of Environmental and Nutrition, Jefferson, AR, United States,3 Division of Health Sciences, Research Triangle Park, NC, United States Biochemical Toxicology, Jefferson, AR, United States P98 Reconstructed 3D Human Skin Comet Assay: Intra- and P80 Improvement of In Vitro Risk Assessment for Carcinogenic Interlaboratory Reproducibility with Five Chemicals Potential: Validation of Defined Genotoxic Thresholds Downs T1, Reus A2, Reisinger K3, Krul C2, Pfuhler S1. 1Procter Chapman KE, Doak SH, Jenkins GJS. DNA Damage Group, & Gamble Co, Cincinnati, OH, United States, 2TNO, Zeist, Swansea University, Swansea, Wales, United Kingdom Netherlands, 3Henkel AG & Co KgaA, Düsseldorf, Germany P82 Characterization of Molecular Events Underlying P100 Interlaboratory Prevalidation of the Reconstructed 3-D Carcinogen-Induced Morphologically Transformed (MT) Human Skin Micronucleus Assay: Preliminary Results Phenotypes in the Syrian Hamster Embryo (SHE-MT) with 28 Coded Chemicals Assay Pfuhler S1, Aardema M1,2, Ouedraogo G3, Barnett B1, Faquet B3, September 10 Monday, Pickles JC1, Yasaei H1, Moore C2, Scott AD2, Newbold RF1. 1Brunel Mun G4, Hewitt NJ5, Hoffmann 6S , Curren R4. 1Procter & Gamble University, Institute for Cancer Genetics and Pharmacogenomics, Co, Cincinnati, OH, United States, 2Marilyn Aardema Consulting, Uxbridge, Middlesex, United Kingdom, 2Unilever Safety & LLC, Fairfield, OH, United States,3 L’Oreal Life Sciences Research, Environmental Assurance Centre (SEAC), Colworth Science Park, Aulnay sous Bois, France, 4Institute for In Vitro Sciences, Inc, Sharnbrook, Bedford, United Kingdom Gaithersburg, MD, United States, 5SWS, Erzhausen, Germany, 6seh consulting+services, Koln, Germany P84 Evaluating ToxCast™ High-Throughput Assays for Their Ability to Detect Direct-Acting Genotoxicants P102 Genotoxicity and Uptake of Titanium Dioxide Kligerman A, Judson R, Houck K. US Environmental Protection Nanoparticles in Salmonella typhimurium Agency, Research Triangle Park, NC, United States Butler K1,2, Rajani A2, Elespuru R2. 1National Cancer Institute, Bethesda, MD, United States, 2US Food and Drug Administration, P86 Pig-a Mutant RBCs in Rats Treated with ENU In Utero Silver Spring, MD, United States Dobrovolsky V, Heflich R, Ferguson S. NCTR, Jefferson, AR, United States P104 Effect of Insecticide Exposure in the Generation of Alterations in MLL: A Gene Associated with Acute P88 Evaluation of In Vivo Genotoxicity of Estragole by Lymphoblastic Leukemia Comet Assay Navarrete-Meneses P1,4, Betancourt M2, Bonilla E2, Altamirano Bishop ME, Ding W, Lyn-Cook LE, Pearce MG, Manjanatha M3, Reyes A1, Pérez-Vera P1. 1Laboratorio de Cultivo de MG. US Food and Drug Administration, Jefferson Laboratories, Tejidos, Instituto Nacional de Pediatría, Mexico City, Mexico, National Center for Toxicological Research, Jefferson, AR, United 2Departamento de Ciencias de la Salud, Universidad Autónoma States Metropolitana-Iztapalapa, Mexico City, Mexico, 3FES-Zaragoza, 4 P90 Flow Cytometric 96-Well Microplate-Based In Vitro UNAM. México, DF, Mexico City, Mexico, Posgrado en Ciencias Micronucleus Assay in TK6 Cells: Interlab Assessment of Biológicas, UNAM, Mexico City, Mexico Robustness and Transferability P106 Development of an In Vivo Micronucleus Assay Using De Boeck M1, Bryce S2, Avlasevich S2, Dertinger S2, Billinton N3, Isolated Epithelial Cells of Rat Gastrointestinal Tract: Tate M 3, Walmsley R3,4, Elhajouji A5, Van Goethem F1. 1Janssen Principle and Methodology for Combination with Bone Research & Development, a Division of Janssen Pharmaceutica Marrow Micronucleus Assay 2 N.V., Beerse, Belgium, Litron Laboratories, Rochester, NY, United Okada E, Narumi K, Fujiishi Y, Fukuda Y, Yasutake N, Ohyama 3 4 States, Gentronix Ltd, Manchester, United Kingdom, Faculty W, Uchida K, Kaneko K. Yakult Central Institute, Yaho, Tokyo, of Life Sciences, University of Manchester, Manchester, United Japan Kingdom, 5Novartis Pharma, Basel, Switzerland P108 Characterization of the Effects of Gamma- P92 Further Developments of the Mammalian In Vitro Pig-A hexachlorocyclohexane on Cell Viability and KISS-1 Gene Mutation Assay Regulation MCF-7 Breast Cancer Cells 1 1 2 3 3 Rees BJ , Jenkins GJ , Lynch AM , Tate M , Walmsley RM , Joseph S, d’Auvergne O. Southern University and A&M College, 1 1 Johnson GE . Swansea University, Swansea, United Kingdom, Baton Rouge, LA, United States 2GlaxoSmithKline, Hertford, United Kingdom, 3Getronix Ltd., Manchester, United Kingdom P110 HPRTdup-GFP Mice: A New Knock-In Mouse System for the In Vivo Visualization of Somatic and Germ Cell P94 Impact of Serum Content on Cell Uptake and Toxicity of Mutants Size-Differentiated Nanoparticles Noda A1, Suemori H2, Hirai Y1, Kodama Y1, Nakamura N1. 1 1 1 2 2 Wills JW , Fish D , Holton MD , Hondow N , Brown A , Summers 1Radiation Effects Research Foundation, Hiroshima, Japan,2 Kyoto 1 1 1 1 HD , Jenkins GJS , Doak SH . Swansea University, Swansea, University, Kyoto, Japan United Kingdom, 2University of Leeds, Leeds, United Kingdom P112 Characterizing the Response Curve at Extremely Low Doses of Cobalt-60 Gamma Radiation in Human Lymphoblastoid Cells Joshi G, Joiner M, Tucker J. Wayne State University, Detroit, MI, United States www.ems-us.org 25 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

P114 Do Neutrons Induce a Bystander Effect in Normal Human P134 Downregulation of Telomerase Activity and Shortening of Lymphoblastoid Cells? Telomere Length by Polychlorinated Biphenyls (PCBs) in Seth I, Tucker J, Joiner M. Wayne State University, Detroit, MI, HL-60 Cells United States Xin X, Perumal Kuppusamy S, Schnoor JL, Ludewig G. The University of Iowa, Iowa City, IA, United States P116 Bioactivation of Aristolochic Acid Monday, 10–Tuesday, September 11 September Sidorenko VS, Attaluri S, Johnson F, Grollman AP. Department of Pharmacological Sciences, Stony Brook University School of 6:00 PM–8:00 PM Medicine, Stony Brook, NY, United States Dinner on Your Own P118 Normal and Pathological Levels of KRAS Mutation: Information with Implications for Personalized Cancer Treatment 6:00 PM–7:00 PM COMMITTEE MEETINGS Myers MB, McKim KL, Wang Y, McKinzie PB, Meng F, Parsons BL. National Center for Toxicological Research, US Food Alexander Hollaender Juniper and Drug Administration, Jefferson, AR, United States Outreach Committee P120 The Fanconi Anemia Pathway Restricts the Human Education, Student and Larch Papillomavirus Life Cycle New Investigator Affairs Committee Hoskins EE1, Morreale RJ1, Werner SP1, Higginbotham JM1, Laimins LA3, Lambert PF4, Brown DR5, Gillison ML2, Nuovo GJ2, Membership and professional Laurel Witte DP1, Kim MO1, Davies SM1, Mehta PA1, Butsch Kovacic Development Committee M1, Wikenheiser-Brokamp KA1, Wells SI 1. 1Cincinnati Children’s Hospital, Cincinnati, OH, United States, 2Ohio State University, 3 Columbus, OH, United States, Feinberg School of Medicine, 7:00 PM–8:00 PM Chicago, IL, United States, 4University of Wisconsin, Madison, WI, United States, 5Indiana University, Indianapolis, IN, United States Special Interest Group Leaders Laurel P122 Modifications to the Human TP53 Knock-In Mouse Fibroblast Immortalisation Assay for Studying TP53 Mutations Induced by Environmental Carcinogens Kucab J1, Luijten M2, van Steeg H2, Phillips D1, Arlt V1. 1King’s College London, London, United Kingdom, 2National Institute of Public Health and the Environment, Bilthoven, Netherlands P124 How Soon after Exposure Are Mutations Induced in an Ames Assay? Wagner V, Hines R, Dakoulas E, Taylor B. BioReliance Corporation, Rockville, MD, United States Tuesday, September 11 P126 Xeroderma Pigmentosum A-Null Cortical Neurons Are Protected from UV-Initiated Apoptosis While Cockayne 7:00 AM–5:00 PM Grand Ballroom Foyer Syndrome B-Null and Wild Type Neurons Are Rescued by REGISTRATION 5-Bromo-2-Deoxyuridine (BrdU) Rajakulendran N, Tamblyn L, Laposa R. University of Toronto, Toronto, ON, Canada 7:00 AM–5:00 PM Grand Ballroom E P128 Mutational Patterns of Human Mitochondrial DNA As Speaker Ready Room Open Revealed by Ultra-Deep Sequencing Kennedy S, Schmitt M, Salk J, Loeb L. University of Washington, Seattle, WA, United States P130 Bioanalytical, Molecular, and Genetic Alterations in Ethylene Oxide-Exposed Male B6C3F1 Mice LeBaron MJ1, Schisler MR1, Zhang F1, Jeong YC1, Bartels MJ1, Sura R1, Hotchkiss J1, Geter DR1, Gollapudi BB1, Moore NP2. 1The Dow Chemical Company, TERC, Midland, MI, United States, 2The Dow Chemical Company, TERC, Horgen, Switzerland P132 Patterns of Copy Number Changes between Spleen and Cerebellum Differ in the Harlequin Mouse Model of Mitochondrial Dysfunction Wishart AE, Eitutis ST, Butler J, Locke B, Daley M, Hill K. The University of Western Ontario, London, ON, Canada

26 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

7:00 AM–8:30 AM BREAKFAST MEETINGS

Breakfast Pickup Evergreen Ballroom Foyer AGENDA for Meetings (Ticket Required—Breakfast Available at 6:45 AM)

Environmental Genetic Evergreen Ballroom A Toxicology Special Interest Group Leaders: Christopher M. Somers, University of Regina and Carol D. Swartz, Integrated Laboratory Systems 7:00 AM Welcome 9:45 AM–12:30 PM Grand Ballroom A SYMPOSIUM 5 7:15 AM Realizing the Value of Indicator Species in Human Health Studies DNA Damage-Mediated Gene Regulation Nathaniel Hitt, USGS Leetown Science Center Chairpersons: Bruce C. McKay, University of Ottawa and

7:45 AM Next Generation DNA Sequencing in Mats Ljungman, The University of Michigan September 11 Tuesday, Ecotoxicology Studies 9:45 AM S21 Upstream and Downstream of Wild Type and Travis Glenn, University of Georgia, Environmental Mutant Forms of p53 Health Science Carol Prives, Columbia University 8:15 AM Election of Co-Chair 10:15 AM S22 Posttranscriptional Regulation of the p53 Risk Assessment Evergreen Ballroom B Response Special Interest Group Bruce C. McKay, University of Ottawa Leaders: David A. Eastmond, University of California, Riverside and 10:45 AM Break (Grand Ballroom Foyer) Nagu Keshava, US Environmental Protection Agency 11:00 AM S23 Regulation of HuR Function by Stress-Response Transgenic and In Vivo Evergreen Ballroom C Kinases Mutagenesis Special Interest Group Miriam Gorospe, National Institute on Aging, NIH Leaders: Mugimane G. Manjanatha and Nan Mei, National Center 11:30 AM S24 Stress-Induced Regulation of the Transcriptome for Toxicological Research, US FDA and RNA Stabilome 7:00 AM General Introduction, Nominations and Jayendra Prasad, University of Michigan Elections for Chair and Co-Chair, Circulate 12:00 Noon S25 Toxtracker Assays: Advanced Mechanism-Based Membership List Update, and New Business GFP Reporter Systems for Toxicity Profiling of 7:15 AM P122 Modifications to the Human TP53 Knock-In Chemicals Mouse Fibroblast Immortalizations Assay Harry Vrieling, Leiden University Medical Center for Studying TP53 Mutations Induced by Environmental Carcinogens Jill E. Kucab, King’s College 9:45 AM–12:30 PM Grand Ballroom I 7:30 AM P74 Micronucleus Assay and Comet Assay Using SYMPOSIUM 6 Human Hepatocyte Chimeric Mouse (PXB- Mechanisms of Germ Cell Aneuploidy: Do mouse®) Environmental Exposures Play a Role? Jin Tanaka, Biosafety Research Center

Chairpersons: Francesco Marchetti, Health Canada and 7:45 AM P115 Detection of Genotoxic and Nongenotoxic Kristine L. Witt, National Institute of Environmental Health Sciences Carcinogens in Xpc-/- p53+/- Mice Joost Melis, Leiden University Medical Center Primary Sponsor: March of Dimes Foundation Grant 4-FY12-536 8:00 AM P110 HPRTdup-GFP Mice: A New Knock-In Mouse System for the In Vivo Visualization of Somatic 9:45 AM S26 Making Crossovers: Meiotic Recombination in and Germ Cell Mutants Human Males and Females Asao Noda, Radiation Effects Research Foundation Terry Hassold, Washington State University 8:15 AM P68 Mutagenicity and DNA Adduct Formation by 10:15 AM S27 Endocrine Disruptors and Gametogenesis Aristolochic Acid in the Spleen of Big Blue Rats Patricia Hunt, Washington State University Xiaoqing Q. Guo, National Center for Toxicological Research, US FDA 10:45 AM Break (Grand Ballroom Foyer) 11:00 AM S28 Sperm Aneuploidy Associated with Lifestyle and Work Exposures 8:30 AM–12:00 Noon Evergreen Ballroom E Wendie A. Robbins, University of California Los POSTERS AND EXHIBITS OPEN Angeles www.ems-us.org 27 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

11:30 AM S29 Xenoestrogens Cause Aneuploidy in the Sperm 3:00 PM 32 Dissecting Chemical Interactions and Impact and Offspring of Fish of DNA Lesions on RNA Polymerase II Irvin R. Schultz, Battelle Pacific Northwest National Transcriptional Fidelity Laboratory Wang D. University of California, San Diego, La Jolla, CA, United States 12:00 Noon S30

Tuesday, 11 September Gene-Environment Interactions in Transplacental Ovarian Toxicity and 3:15 PM 33 The Role of Reactive Oxygen Species in Cisplatin Tumorigenesis Cytotoxicity Ulrike Luderer, University of California, Irvine Marullo R1,2, Degtyareva NP1, Werner E1, Ramalingam SS1, Doetsch PW1. 1Emory University, Atlanta, Georgia, United States, 2University of 12:30 PM–2:00 PM Messina, Messina, Italy Lunch on Your Own 3:30 PM 34 Digital Quantification of Random Mitochondrial Deletions Taylor SD 1, Ericson NG1, Prolla TA2, Bielas JH1. 12:30 PM–2:00 PM Evergreen Ballroom A 1Fred Hutchinson Cancer Research Center, Seattle, 2 Women in the EMS Special Interest Group WA, United States, University of Wisconsin- Lunch Meeting Madison, Madison, WI, United States (Advance Registration Required) 3:45 PM 35 Profiling DNA Damage Pathways Activated by Chemicals with Different Mechanisms of DNA Contributing Sponsor: National Institute of Environmental Damage Health Sciences Clewell R1, Sun B1, Thomas 1A , Carmichael P2, White 2 1 1 Leaders: Barbara L. Parsons, National Center for Toxicological A , Andersen M . The Hamner Institutes for Health Research, US FDA and Kristine L. Witt, National Institute of Sciences, Research Triangle Park, NC, United States, 2Unilever, Colworth Science Park, United Kingdom Environmental Health Sciences 12:30 PM Networking 2:00 PM–4:00 PM Grand Ballroom I 12:45 PM New Business Platform Session 6 1:00 PM The Conquest of Cancer through Research Chromosome Instability Julia Carter, Wood Hudson Cancer Research Laboratory Chairpersons: Ofelia A. Olivero, National Cancer Institute, NIH and Martin Arlt, University of Michigan 1:45 PM Discussion Underlined author indicates presenter. 2:00 PM–4:00 PM Grand Ballroom A 2:00 PM 36 Chromosomal Instability and Aneuploidy Induced by Nucleoside Analogs Platform Session 5 Ofelia A. Olivero, National Cancer Institute, NIH Mutagenesis, Environmental Genomics, and Cancer 2:30 PM 37 Mre11 Interacts with Cyclin-Dependent Kinase 2 to Regulate Double-Strand Break Repair Chairpersons: Lawrence A. Loeb, University of Washington and Ferguson D. The University of Michigan Medical Dong Wang, University California, San Diego School, Ann Arbor, MI, United States Underlined author indicates presenter. 2:45 PM 38 Separation of Intra-S Checkpoint Protein Contributions to DNA Replication Fork 2:00 PM 29 Cancer: An Evolving Challenge Protection and Genomic Stability in Human Lawrence A. Loeb, University of Washington Fibroblasts 2 1 3 3 2:30 PM 30 Mutation Rate As a Therapeutic Target in Cancer Smith-Roe S , Patel S , Zhou Y , Simpson D , Rao 4 4 4 5 1 1 1 S , Ibrahim J , Cordeiro-Stone M , Kaufmann W . Fox EJ , Shen J-C , Prindle MJ , Kaur-Rayar 1 1 2 1 1 University of North Carolina at Chapel Hill, Chapel J , Essigmann JM, , Loeb LA . Departments 2 of Biochemistry and Pathology, University Hill, NC, United States, Department of Genetics, University of North Carolina at Chapel Hill, Chapel of Washington, Seattle, WA, United States, 3 2Department of Chemistry, Massachusetts Institute Hill, NC, United States, Department of Pathology of Technology, Boston, MA, United States and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United 2:45 PM 31 Mutational Consequences of Altered DNA States, 4Department of Biostatistics, University of Precursor Pools North Carolina at Chapel Hill, Chapel Hill, NC, Schaaper RM, Ahluwalia D, Bienstock RJ. National United States, 5Lineberger Comprehensive Cancer Institute of Environmental Health Sciences, Research Center, Chapel Hill, NC, United States Triangle Park, NC, United States

28 www.ems-us.org Bellevue, Washington • September 8–12, 2012 AGENDA

3:00 PM 39 The Lowest Detectable Dose of Ionizing Radiation by the Cytokinesis-Block Wednesday, September 12

Micronucleus Method AGENDA Tucker J1, Vadapalli M1, Joiner M1, Fenech M2, 7:00 AM–11:30 AM Grand Ballroom Foyer 3 1 Bonassi S . Wayne State University, Detroit, REGISTRATION MI, United States, 2CSIRO Food and Nutritional Sciences, Adelaide BC, South Australia, Australia, 3 Clinical and Molecular Epidemiology, IRCCS San 7:00 AM–11:00 AM Grand Ballroom E Raffaele Pisana, Rome, Italy Speaker Ready Room Open 3:15 PM 40 Telomere Dysfunction and DNA Repair Deficiency: Markers of Sensitivity to Mutagens and Carcinogens? 7:00 AM–8:30 AM COMMITTEE MEETINGS Lim HK1, Hande MP1,2. 1National University of 2013 Program Committee Larch Singapore, Singapore, 2Tembusu College, NUS, Singapore (Second Meeting) 3:30 PM 41 De Novo CNV Formation in Mouse Embryonic Finance and Resource Committee Laurel Stem Cells Occurs in the Absence of XRCC4- Dependent Nonhomologous End Joining Nominating Committee Juniper Arlt M, Rajendran S, Birkeland S, McSweeney K, Wilson T, Glover T. University of Michigan, Ann Arbor, MI, United States 8:30 AM–11:15 AM Grand Ballroom A 3:45 PM 42 Male Germ Cells Mediate Untargeted Mutations SYMPOSIUM 7 to the Maternal Genome of Offspring: Management of the DNA Damage Response Comparison of Transgenic Rodent and Fish Models Chairpersons: Andrew B. Buermeyer, Oregon State University and Gresham CS1, Norris MB1, Alworth LC2, Winn RN1. Paul Doetsch, Emory University School of Medicine 1Interdisciplinary Toxicology Program and Aquatic 8:30 AM S31 Targeting Gene Therapy: Without Leaving Biotechnology and Environmental Laboratory, September 12 September 11–Wednesday, Tuesday, Athens, GA, United States, 2Office of the Vice Footprints President for Research, University of Georgia, Nancy Maizels, University of Washington Athens, GA, United States 9:00 AM S32 Regulation of DNA Base Excision Repair Paul Doetsch, Emory University School of Medicine 9:30 AM Break (Grand Ballroom Foyer) 9:45 AM S33 Human Disease As a Model for Mismatch Repair Mechanism Christopher D. Heinen, University of Connecticut Health Center 10:15 AM S34 When DNA Damage Is Essential to Life: Breaking and Repairing DNA in Meiosis Naina Phadnis, University of Washington 10:45 AM S35 The O6-Alkylguanine Response: Mechanisms and

Implications for Cancer Therapy Bernd Kaina, University of Mainz

8:30 AM–11:15 AM Grand Ballroom I SYMPOSIUM 8 Health Effects in Heavily Polluted Areas Chairpersons: Radim Sram, Institute of Experimental Medicine and 7:00 PM–12:00 Midnight Evergreen Ballroom E Carol D. Swartz, Integrated Laboratory Systems, Inc. Banquet 8:30 AM S36 Ecological Indicators of Human Cancer Awards Presentation by Catherine B. Klein Mortality Rates: New Tools for Exposure and Risk Modeling Alexander Hollaender Award Nathaniel P. Hitt, US Geological Survey Leetown EMS Award Science Center EMS Service Award EMS Emerging Scientist Travel Awards 9:00 AM S37 Hot Spot B[a]P Exposure in the Czech Republic Radim Sram, Institute of Experimental Medicine Student and New Investigator Travel Awards www.ems-us.org 29 AGENDA 43rd Annual Meeting of the Environmental Mutagen Society AGENDA

9:30 AM Break (Grand Ballroom Foyer) 9:45 AM S38 Investigating the Consequences of Metal Contamination in Yellow Perch Using an Integrated Physiological, Transcriptomic, and Genomics Approach Wednesday, 12 September Patrice Couture, Centre Eau Terre Environment 10:15 AM S39 Linxian China: A Population with High Incidence of Esophageal Cancer and Multiple Adverse Exposures Christian Abnet, National Cancer Institute 10:45 AM S40 Health Risks Form Long-Term Exposure to 12:30 PM–12:35 PM Grand Ballroom I Creosote in Bossier City Patricia L. Williams, University of New Orleans Close of the Meeting Mats Ljungman, The University of Michigan

30 www.ems-us.org eMs Announcements special interest group • Monthly editor’s choice information Announcements • scientific images • president’s corner and Reports • newsletter • sig discussion Forum • FAseB updates it is easy to be a sig member— click the Join button within the sig community. Meet the candidates • candidate Biographical information A place to conduct committee Business • distribution lists to Files of interest communicate with • Minutes of executive Board committee Members and council Meetings • Key Reference Files such As • committee Reports sops and other guidance • Business Meeting documents presentations • EMS Connect tips and guidelines • career opportunities

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www.ems-us.org 31 EXHIBITORS 43rd Annual Meeting of the Environmental Mutagen Society EXHI B ITORS Exhibits

Exhibits and Posters are located in Evergreen Ballroom E and will be open the following times:

Evergreen B allroom E | Sunday 4:00 PM–6:00 PM | Monday 8:30 AM–6:00 PM | Tuesday 8:30 AM–12:00 Noon Sunday, September 9 4:00 PM–6:00 PM Monday, September 10 8:30 AM–6:00 PM Tuesday, September 11 8:30 AM–12:00 Noon Agilent Technologies Booth 3 Angelyn Regala Tel: 800.227.9770 5301 Stevens Creek Blvd Fax: 302.239.4810 Santa Clara, CA 95051 Email: [email protected] United States Website: www.agilent.com/genomics Agilent’s portfolio includes the leading SureSelect and HaloPlex Target Enrichment Next-Generation Sequencing Platforms, Gene Expression and Cytogenetic Microarrays, GeneSpring and Cytogenomics Software, qPCR, PCR, Mutagenesis, Cloning, and SureFISH probes. Agilent offers the Bioanalyzer & TapeStation instruments for sample QC, and Automation for optimized workflow productivity. BioReliance Corporation Booth 1 Scott Hickman Tel: 800.756.5658 14920 Broschart Road Fax: 301.738.2362 Rockville, MD 20850 Email: [email protected] United States Website: www.bioreliance.com BioReliance is a leading provider of outsourcing services for pharmaceutical and biopharmaceutical companies, as well as chemicals, consumer products and medical devices. Specializing in mutagenicity testing, our scientific staff and state-of-the-art facilities provide the most complete and comprehensive toxicology resources available anywhere. FASEB MARC Program Booth 10 Cordelia Adams Tel: 301.634.7930 9650 Rockville Pike Fax: 301.634.7253 Bethesda, MD 20814 Email: [email protected] United States Website: www.faseb.org/marc FASEB MARC Program provides a variety of activities to support the training of minority students, postdoctorates, faculty, and scientists in the biomedical and behavioral sciences. We offer travel awards for scientific meetings, research conferences, and student summer research opportunities programs. We also sponsor Career Development Programs including grantsmanship training seminars. Helix3 Inc. Booth 6 Marie Vasquez Tel: 919.462.8105 100 Southcenter Court, Suite 900 Fax: 919.462.8106 Morrisville, NC 27560 Email: [email protected] United States Website: www.helix3inc.com Helix3 is a contract research laboratory specializing in providing GLP comet assays and investigative/mechanistic studies for regulatory submissions. With over 15 years experience applying our innovative approaches to safety testing and risk assessment, Helix3 is the leader in providing reliable and efficient GLP investigative services.

32 www.ems-us.org Bellevue, Washington • September 8–12, 2012 EXHIBITORS

Illumina Booth 2 EXHI B ITORS

Robin Ball Tel: 858.202.4500 5200 Illumina Way Email: [email protected] San Diego, CA 92122 Website: www.illumina.com United States Illumina, the leader in next generation DNA sequencing and microarray analysis, develops product solutions that address every scale of genomic research. Our broad portfolio of leading-edge instruments, reagents, and software offer scientists the best solution for their research. IntelliCyt Corporation Booth 7 Joe Zock Tel: 505.345.9075 9620 San Mateo Boulevard NE Fax: 866.782.3140 Albuquerque, NM 87113 Email: [email protected] United States Website: www.intellicyt.com IntelliCyt Corporation develops and markets innovative high-throughput cell and bead-based screening solutions for use throughout the life sciences. The company uses proprietary technologies to create instrumentation, reagent kits, and software products that dramatically increase the speed and decrease the cost of detecting and measuring cells and beads. Leadscope Incorporated Booth 9 Heather Landon Tel: 614.675.3730 1393 Dublin Road Fax: 614.675.3732 Columbus, OH 43215 Email: [email protected] United States Website: www.leadscope.com Leadscope licenses high quality toxicity databases and QSAR models developed through our CRADA with the US FDA. The databases and models allow scientists to predict the potential toxicity and adverse human clinical effects of pharmaceuticals (including impurities), cosmetics, food products, and chemicals. The Models, built by the US FDA, employ closed training sets prepared using both proprietary and nonproprietary data. Molecular Toxicology, Inc. Booth 4 John Rundell Tel: 828.264.9099 PO Box 1189 Fax: 828.264.0103 Boone, NC 28607 Email: [email protected] United States Website: www.moltox.com MOLTOX® S9, NADPH RegensysTM, ST-and ECDiscTM culture inocula, ControlChemTM positive controls, MGA dishes, and top agars are recognized worldwide for their performance, quality and value. New genetox products include MutazymeTM, ISO13829-compliant SOS/umu test kits, MoltoxFTTM and BioReliance® Ames II microplate-fluctuation assay kits, Comet Assay and in vitro mammalian cell MN test reagents and kits. Perceptive Instruments LTD. Booth 12 Gary Kyle Tel: 44.1440.730.773 Blois Meadow Business Centre Fax: 44.1440.730.630 Steeple Bumpstead Email: [email protected] Evergreen Ballroom E | Sunday 4:00 PM–6:00 PM | Monday 8:30 AM–6:00 PM | Tuesday 8:30 AM–12:00 Noon Tuesday | 8:30 AM–6:00 PM | Monday PM 4:00 PM–6:00 E | Sunday Ballroom Evergreen Haverhill Suffolk Website: www.perceptive.co.uk United Kingdom Perceptive Instruments develops and markets innovative software solutions for genetic toxicology assays. These include automatic colony counting systems for Ames and MLA, as well as image analysis systems for UDS and Comet assays. We also create comprehensive study management tools such as the Ames Study Manager for conducting and reporting regulatory tests. All our systems are designed for compliance with GLP and 21 CFR part 11.

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36 www.ems-us.org Sustaining Members and Institutional Representatives Silver

Boehringer Ingelheim Pharmaceuticals, Inc. Matthew S. Bogdanffy Bronze

Abbott Laboratories GlaxoSmithKline John J. Nicolette Anthony Mark Lynch DuPont Haskell Global Centers for Pfizer Inc. Health and Environmental Sciences Geralyn L. DeVito E. Maria Donner The Procter & Gamble Company Stefan J. Pfuhler

EMS 2012 Thank You

EMS sincerely appreciates the effort and hard work of the following people who have helped make this a successful and worthwhile meeting.

2012 EMS Executive Board William Kaufmann Photographers Nagu Keshava Jeffrey L. Schwartz 2012 Program Committee Mats Ljungman Sandy K. Weiner Lawrence A. Loeb Mugimane G. Manjanatha Other Key Individuals: Special Interest Group Leaders Francesco Marchetti Tonia M. Masson Bruce Campbell McKay Becca Isakower Workshop, Symposium, and Suzanne M. Morris Maureen Kettering Platform Chairpersons: Heather M. O’Hagan Rachel Frohberg Martin Arlt Ofelia A. Olivero Marina V. Bakhmutsky Michael J. Plewa Steven M. Bryce Matias S. Attene Ramos Andrew B. Buermeyer Leona D. Samson Sharon B. Cantor Jeffrey L. Schwartz Matthew A. Coleman Radim J. Sram Priscilla K. Cooper Carol D. Swartz David M. DeMarini Paul Van Hummelen Stephen D. Dertinger Bennett Van Houten Krista L. Dobo Sundar Venkatachalam Paul Doetsch Dong Wang Dana C. Dolinoy Kristine L. Witt Bevin P. Engelward Patricia A. Escobar Philip C. Hanawalt Robert H. Heflich Ana Luisa Kadekaro DNA Repair Women in EMS

EMS Applied Genetic Toxicology Agents Heritable Mutation & Disease Target Embracing the Science of the Future Source EMS Transgenics & Environmental Genetic Research Education In Vivo Mutagenesis through Cross-Disciplinary Research Toxicology Policy Outcome Collegiality

Process Risk Assessment Epigenetics Molecular Epidemiology New Technologies

44th Annual Meeting September 21–25, 2013 Monterey, California at the Hya Regency

Environmental Mutagen Society 1821 Michael Faraday Drive Suite 300 Reston, VA 20190-5348 Cert no. XXX-XXX-XXX X Email: [email protected] EMS Website: www.ems-us.org www.ems-us.org/am2013