Development of Ozone Technology Fish Storage Systems for Improving Quality Fish Production
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View metadata, citation2014 Internationaland similar papers Symposium at core.ac.uk on Technology Management and Emerging Technologies (ISTMET 2014), May 27 - 29, 2014,brought to you by CORE Bandung, Indonesia provided by Diponegoro University Institutional Repository Development of Ozone Technology Fish Storage Systems for Improving Quality Fish Production 1* 1 1 Muhammad Nur , Maryam Resti , Fajar Arianto , Tri A. Winarni4, Susilo5, and Harjum Muharam6 Zaenul Muhlisin1,Sosiowati Teke1, Ade Ika Susan1, 4Department of Fishery Product Technology, Faculty of Endang Kusdiyantini2 Wuryanti3 1 2 Fisheries and Marine Science, Diponegoro University Department of Physics, Department of Biology and 5 Department of Mechanical Engineering, Faculty of 3Department of Chemistry Faculty of Science and Engineering, Diponegoro University Mathematics 6 Department of Management, Faculty of Business and Diponegoro University Economics Diponegoro University Semarang, Indonesia Semarang, Indonesia E-mail: [email protected] Abstract- Development of ozone technology fish storage system marine fisheries, Indonesia also has great potential for (OTFIS) to maintain the quality of the fish has been done. The freshwater fisheries. The sector of freshwater fish aquaculture ozone technology fish storage system (OTFIS) can be applied in in Indonesia has the potential to be developed through the the fishing boat, fish storage in the Fish Auction Market, fish extension and intensification. The commodities of freshwater storage in the means transport for fish. In this research, we fish farming such as catfish, carp, tilapia, and freshwater produced a prototype that was integrated by the fish container and ozone generator. We used Dielectric Barrier Discharge ornamental fish have a fairly high demand in domestic and Plasma (DBDP) technology for ozone generator. The export markets. Export markets demand a positive impact on characterization of ozone generator has been carried out and the increase in Indonesia’s foreign exchange. showed that ozone generator can produce ozone concentration The fishery export is closely related to the quality of fish from 0.5 ppm to 12 ppm. The ozone concentration in the area is delivered, while the quality of the fish was associated to fish very suitable to be used in food technology. The ozone treatment storage technology from the whole process of traveling fish was performed on red tilapia representing the white-fleshed fish caught up to the consumer. Currently, there are several with ozone concentrations from 0.5 ppm to 4.5 ppm. The results methods for preserving fish using methods including drying, of this treatment showed that organoleptic and TVBN of fish curing, salting, fermentation, and the most commonly used is were still appropriate with Indonesian National Standard (INS). Beside that, TPC testing has been done on the red tilapia showed cooling. Most people in ways that can be harmful to health, for that the higher the ozone concentration the smaller the remaining example by using formalin for preservation of fish. of bacteria colonies. Similarly, for a variable of time, the longer From the above discussion it can be concluded that, fish the exposure to ozone the less the remaining of colonies. The storage technology has not been able to keep the quality of the results of this study have been adapted to realize of OTFIS with a fish. The new technology is needed to resolve the issue. The size of 50 cm x 50 cm x 100 cm (250 liters) and can accommodate alternative is ozone technology. The utilization of appropriate as much as 100 kg of fish. ozone technology and ozone dose right is believed to be used for storage technology in the whole his journey process begin Keywords: fish, storage, quality, plasma, ozone caught to the consumer. The ozone technology has been developed for the water sterilization process, vegetable or I. INTRODUCTION food, medical equipment, and various other applications. In The Indonesian marine fisheries potential spread in 1995, ozone was declared safe by Generalize Recognized as nearly all parts of the existing Indonesian waters as the Safe (GRAS) or was generally recognized to be safe by the territorial sea of the archipelago and the waters of Exclusive FDA (U.S. Food and Drug Administration) for the treatment Economic Zone (EEZ). The Indonesian sea area is estimated of drinking water bottles. These applications are then at 5.8 million km2 with the longest coastline in the world at developed as "GRAS" for food processing by some experts the 81.000 km and the cluster of islands as much as 17.508. This last few years [2]. geographical situation has the potential of fish estimated as Ozone can be produced using two electrodes fed by high many as 6.26 million tons per year. This amount can be voltage with gas fill between the two electrodes in the form of detailed as much as 4.4 million tons can be caught in the pure oxygen gas or air. Between the two electrodes is covered waters of Indonesia and 1.86 million tons can be obtained by a dielectric material in order to avoid the occurrence of arc from Indonesian Exclusive Economic Zone (IEEZ) waters. discharge. The factors that can affect ozone formation in According to the Ministry of Maritime and Fisheries, the general are voltage, dielectric materials, the pressure, the Indonesian marine fisheries nominal value of 57.69 trillion configuration system of the plasma reactor, and a gas in the IDR, or the equivalent of 6.48 percent of GDP in 2012, while plasma reactor [3,4,5]. Ozone formation involves mechanism the value of economic activity around 255.3 billion IDR. The of ionization and recombination both dissociation and potential to grow by 7 percent if compared to the second association. The reaction of ozone formation can be occured in quarter 2013 to the second quarter of 2012 [1]. In addition to a plasma reactor [6]. 978-1-4799-3704-2/14/$31.00 ©2014 IEEE 167 Bacteriocidal effect (substances that can kill bacteria) of points of irrigation in Epirus. Voidarou proved a decrease of ozone has been tested against a variety of organisms, resistance in the E. coli and Enterococcus sp after ozone including Gram-positive bacteria (Listeria monocytogenes, treatment of 30 minutes,with ozone concentration in the range Staphylococcus aureus, Bacillus cereus, Enterococcus from 0.15 to 0.20 mg/lt . Voidarou also wrote some of the faecalis), Gram-negative (Pseudomonas aeruginosa and most important factors that influence the process of Yersinia enterocolitica give), yeast (Candida albicans give inactivation of E. coli, namely: ozone concentration, exposure Zygosaccharomyces Bacilli), spore (Aspergillus niger) and time, and RH. vegetative cells. Microbial inactivation is a complex process. Utilization of ozone have been made to improve the Ozone can affect glycoproteins and or glycolipids membrane , safety and quality of salmon [13]. Quality of sardines can be the binding of membrane enzyme, and may be able to damage maintained properly in a long time through a combination of proteins and DNA. Microorganisms is attacked through the ozone treatment and ice [14]. process of disruption (disorder) in the cell or disintegration Ozone was reported to be effective in detoxifying and that causes lysis process of the cells. Lysis process by ozone is eliminating mold and mycotoxins such as aflatoxins, patulin, a fast inactivation mechanism compared to other disinfectants cyclopiazonic acid, D secalonic acid, and ochratoxin A. The that require disinfecting agent to permeate through the cell factors that determine the ability of ozone to kill insects, rot membrane [2,7,8]. causing bacteria in food, removal toxic mold, and According to Voidarou 2006 [2], microorganisms and decontamination depends among others, ozone concentration, mycotoxin decontamination process depends on the exposure time, and pH [8.11,15]. Utilization of ozone and concentration of ozone given, exposure time, pH, and moisture refrigeration for the storage of fish has been able to maintain content. Disinfecting properties of ozone has been tested by the quality of fish for example was reported by [14, 16, 17, several researchers. For example, Tiwari et. al., 2009 [8], 18,19]. which examined the effectiveness of ozone against the grain Ozone is able to inhibit the process of decay in fish. The pests such as Tribolium castaneum and Ephestia elutella and process of fish preserving in principle is the inhibition of the Coliform bacteria, Micrococcus, Bacillus spores ,fungi, spoilage in fish. Immediately after the fish dies, there will be and so on. In his report [8], Tawari et. al. wrote that the changes that lead to decay. The changes are primarily due to respiratory system to be the entrance of ozone into the body of the activity of enzymes, chemicals, and bacteria. Enzymes the insect. Ozone causes oxidative tissue damage (even at low contained in the fish body will remodel the body parts of fish concentrations), DNA damage, and changes in the pulmonary and resulting changes in taste (flavor), smell (odor), form system, bronchial sensitivity, membrane oxidation or in vivo (appearance), and texture. Chemical activity is the meat fat mutation. Increased respiratory system with increased oxidation by oxygen. The oxygen contained in the air temperature can result in excessive gas exchange due to an oxidizing fatty fish meat and causes the rancid smell. increase in metabolism and respiration rate. Changes caused by bacteria triggered by the occurrence In a different study [9], Ran et al., test the effectiveness of damage to the components in fish by enzyme and chemical of ozone against Criptosporidium (intestinal protozoa, one of activity. Chemical activity produces simpler components. This the causes of diarrhea) and the Giardia parasite protozoan. In condition is preferred bacteria that trigger the growth of addition, Ran also investigated the mechanism of bacteria on the body of fish. In fact, the process of quality Cryptosporidium cell destruction by ozone using scanning deterioration is very complex ongoing.