SET07 Antibody / KMT5A [Clone 43AT551.86] (F40198)

SET07 Antibody / KMT5A [clone 43AT551.86] (F40198)

Catalog No. Formulation Size

F40198-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml

F40198-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml Bulk quote request

Availability 1-3 business days

Species Reactivity Human, Mouse

Format Purified

Clonality Monoclonal (mouse origin)

Isotype Mouse IgG1

Clone Name 43AT551.86

Purity Purified

UniProt Q9NQR1

Applications Western blot : 1:1000 IHC (Paraffin) : 1:10-1:50 Immunofluorescence : 1:10-1:50

Limitations This SET07 antibody is available for research use only.

Western blot analysis of 293 lysate using SET07 antibody at 1:1000. IHC analysis of FFPE human testis tissue stained with SET07 antibody

Immunofluorescence analysis of SET07 antibody and HeLa cells with (bottom) and without (top) PE-conjugated goat anti-mouse lgG secondary.

Description SET07 is a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. [UniProt]

Application Notes Titration of the SET07 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.

Storage Aliquot the SET07 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.