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PR-Set7 and H4k20me1: at the Crossroads of Genome Integrity, Cell Cycle, Chromosome Condensation, and Transcription

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PR-Set7 and H4k20me1: at the Crossroads of Genome Integrity, Cell Cycle, Chromosome Condensation, and Transcription Downloaded from genesdev.cshlp.org on October 5, 2021 - Published by Cold Spring Harbor Laboratory Press REVIEW PR-Set7 and H4K20me1: at the crossroads of genome integrity, cell cycle, chromosome condensation, and transcription David B. Beck,1,5 Hisanobu Oda,2,3,5 Steven S. Shen,1,4 and Danny Reinberg1,6 1Howard Hughes Medical Institute, Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA; 2Gastrointestinal and Medical Oncology Division, National Kyushu Cancer Center, Fukuoka 811-1395, Japan; 3Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan; 4Center for Health Informatics and Bioinformatics, Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA Histone post-translational modifications impact many candidates for the means to propagate gene expression aspects of chromatin and nuclear function. Histone H4 states epigenetically. Lys 20 methylation (H4K20me) has been implicated in Histone H4 Lys 20 (H4K20) was one of the earliest regulating diverse processes ranging from the DNA damage modified histone residues to be identified, found in both response, mitotic condensation, and DNA replication to pea and calf extracts in 1969 (DeLange et al. 1969). By gene regulation. PR-Set7/Set8/KMT5a is the sole enzyme analyzing metabolically labeled histone tails, H4K20 was that catalyzes monomethylation of H4K20 (H4K20me1). It subsequently identified as a major site of in vivo meth- is required for maintenance of all levels of H4K20me, and, ylation, and it is the only identified and characterized importantly, loss of PR-Set7 is catastrophic for the earliest methylated lysine residue on the H4 N-terminal tail stages of mouse embryonic development. These findings (Zhang and Reinberg 2001). In metazoans, H4K20 can be have placed PR-Set7, H4K20me, and proteins that recog- modified to three separate degrees: mono-, di-, and tri- nize this modification as central nodes of many important methylation. These different methylation states are con- pathways. In this review, we discuss the mechanisms trolled by distinct methyltransferases in vivo, although a required for regulation of PR-Set7 and H4K20me1 levels number of other enzymes have similar activity in vitro and attempt to unravel the many functions attributed to (Beisel et al. 2002; Marango et al. 2008; Schotta et al. 2008; these proteins. Oda et al. 2009). Separate enzymatic activity specific for only monomethylation of H4K20 (H4K20me1) is present in Caenorhabditis elegans, whereas lower eukaryotes such as Schizosaccharomyces pombe contain a single Post-translational modifications of histones have methyltransferase for all H4K20 methylation (H4K20me) emerged as an important means by which the cell can states, while Saccharomyces cerevisae contains little to no demarcate specific regions of the genome for differential H4K20me at all (Wang and Jia 2009; Balakrishnan and and dynamic regulation (Campos and Reinberg 2009). Milavetz 2010; Edwards et al. 2011). Thus, the more recent These modifications, such as phosphorylation, methyla- appearance of H4K20me1 suggests an expansion of the tion, acetylation, and ubiquitination, occur extensively chromatin modification canon in higher eukaryotes. on the N-terminal tails of histones and have diverse In mammals, at least three methyltransferases (PR-Set7, functions and modes of action. A substantial amount of Suv4-20h1, and Suv4-20h2) and one demethylase (PHF8) work has sought to determine how these ‘‘marks’’ regu- directly regulate H4K20 methylation (Fig. 1; Liu et al. 2010; late gene expression, but histone modifications have also Qi et al. 2010). PR-Set7/Set8/KMT5a is the sole monometh- been linked to other cellular processes, such as mitosis yltransferase (Oda et al. 2009), whereas Suv4-20h1/h2 (Hirota et al. 2005; Kelly et al. 2010; Oda et al. 2010; Wang catalyzes the transition from H4K20me1 to H4K20me2/3 et al. 2010; Yamagishi et al. 2010), meiosis (Govin et al. (Schotta et al. 2008). PHF8 demethylates H4K20me1 to 2010), and the DNA damage response (Burma et al. 2001; H4K20me0, while a demethylase for H4K20me2/3 re- Polo and Jackson 2011). Furthermore, despite a lack mains to be identified (Liu et al. 2010; Qi et al. 2010). of clear evidence, histone modifications are leading Although separate enzymes control each level of methyla- tion, H4K20me2/3 are dependent on H4K20me1. Further- [Keywords: PR-Set7; H4K20me1; chromatin; cell cycle; DNA damage] more, each of the H4K20me states exhibits unique genomic 5These authors contributed equally to this work. distributions and is recognized by specific binding proteins, 6Corresponding author. E-mail [email protected]. underscoring its distinct roles. Regulation of H4K20me is Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.177444.111. essential, since knockout studies have shown that PR-Set7 GENES & DEVELOPMENT 26:325–337 Ó 2012 by Cold Spring Harbor Laboratory Press ISSN 0890-9369/12; www.genesdev.org 325 Downloaded from genesdev.cshlp.org on October 5, 2021 - Published by Cold Spring Harbor Laboratory Press Beck et al. Figure 1. Overview of H4K20me. (A)Schematicdia- gram of the different methylation states of histone H4K20; from left to right: unmethylated (H4K20), mono- methylated (H4K20me1), dimethylated (H4K20me2), and trimethylated (H4K20me3), with the various mam- malian enzymes that perform these post-translational modification. (B) Properties of each of the enzymes that affect H4K20 methylation. and at least one of Suv4-20h1/2 are required for devel- that the lack of PR-Set7 catalytic activity causes a growth opment in mice and that loss of these enzymes causes arrest due to increased DNA damage and defects in cell increased DNA damage and cell cycle defects (Schotta cycle progression in embryonic stem (ES) cells (Oda et al. et al. 2008; Oda et al. 2010). Not surprisingly, loss of the 2009). The later lethality in the case of fly embryos may be monomethyltransferase PR-Set7 causes a more severe and explained by increased maternal contribution of PR-Set7 complex phenotype, as this precludes the catalysis of all transcript, possibly delaying depletion of H4K20me1. levels of H4K20 methylation. We hypothesize that the primary function of PR-Set7 PR-Set7 was initially identified as a H4K20 monometh- and the requirement for enzymatic activity is regulation yltransferase by unbiased conventional biochemical pu- of H4K20me levels. Both PR-Set7 and H4K20me1 oscil- rification (Nishioka et al. 2002). PR-Set7 displays robust late during the cell cycle, and maintenance of H4K20me enzymatic activity toward nucleosomal substrates, but levels is critical for proper cell cycle progression through not histone octamers or histone H4 alone. PR-Set7 was its role in protecting genome stability, DNA replication, shown to be strictly a monomethyltransferase through mitosis, and transcription. The emergence of PR-Set7 a combination of nuclear magnetic resonance (NMR), during evolution suggests a role specific to higher eukary- mass spectroscopy, and in vitro experiments (Couture otes, which may be related to the increased complexities of et al. 2005; Xiao et al. 2005). Providing further support mitosis, DNA replication initiation, or gene regulation. and general insight as to how methyltransferases can be However, why PR-Set7 methyltransferase activity is es- monomethyl-specific, crystallographic studies demon- sential for cell viability in higher eukaryotes is still only strated that the substrate recognition channel of the partially understood. Despite many thorough investiga- enzyme is too narrow to accommodate a trimethylated tions into the function of PR-Set7, we believe that we are species. This structure also helped determine that PR-Set7 only now on the cusp of fully understanding the role of acts specifically at H4K20 due to an array of required con- H4K20me1. In the following sections, we first discuss the tacts with the surrounding residues on H4. substrates and regulation of PR-Set7, followed by its role in PR-Set7 catalytic activity is essential for viability. In proper cell cycle progression, the DNA damage response, Drosophila, loss of PR-Set7 causes lethality at the third gene regulation, and higher-order chromatin structure. instar larval stage, potentially through defects in embry- onic patterning (Karachentsev et al. 2005). In mice, PR- PR-Set7 substrates and effectors Set7 knockout has a much more severe phenotype and results in embryonic lethality and arrest between the Although other yet-to-be-identified substrates may exist, four- and eight-cell stage (Oda et al. 2009). Rescue exper- methylation of H4 is likely to be the major effector of PR- iments in mouse embryos elegantly showed that the lack Set7 function and a crucial mediator of its impact on of catalytic activity of PR-Set7 causes the lethality of PR- viability. As is potentially the case for other histone Set7À/À embryos. Injection of wild-type PR-Set7 mRNA modifications, H4K20me could act directly through alter- into one of the PR-Set7À/À blastomeres at the two-cell ing nucleosome structure or indirectly through specific stage led to blastocoel cavity formation from the progeny binders that mediate downstream functions. These two of injected cells, which was not observed for the progeny of possibilities are further complicated in the case of PR-Set7, cells that were injected with mRNA encoding a catalyti- since the loss of H4K20me1 also causes a decrease in cally inactive mutant of PR-Set7. Further analysis showed H4K20me2/3. However, the phenotype of Suv4-20h1/2À/À 326 GENES & DEVELOPMENT Downloaded from genesdev.cshlp.org on October 5, 2021 - Published by Cold Spring Harbor Laboratory Press PR-Set7 and H4K20me1 mice (Suv4-20 double knockout) is much less severe lication (Jorgensen et al. 2007; Huen et al. 2008). This in- than PR-Set7À/À mice, suggesting a unique function for teraction recruits PR-Set7 to sites of DNA damage and H4K20me1 independent of H4K20me2/3 (Schotta et al. replication and is also essential for the CRL4Cdt2-dependent 2008). degradation of PR-Set7 (Abbas et al. 2010; Centore et al.
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