FASTK Rabbit Pab

Leader in Biomolecular Solutions for Life Science FASTK Rabbit pAb Catalog No.: A7069 Basic Information Background Catalog No. The protein encoded by this gene is a member of the serine/threonine protein kinase A7069 family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis- Observed MW promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of 61kDa lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been Calculated MW determined. 45kDa/57kDa/58kDa/61kDa Category Primary antibody Applications WB,IHC,IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 10922 Q14296 IHC 1:50 - 1:200 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 360-549 of human FASTK (NP_006703.1). Synonyms FASTK;FAST Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using FASTK antibody (A7069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunofluorescence analysis of U2OS embedded rat liver using FASTK antibody embedded human kidney using FASTK cells using FASTK antibody (A7069). Blue: (A7069) at dilution of 1:100 (40x lens). antibody (A7069) at dilution of 1:100 (40x DAPI for nuclear staining. lens). Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Gene Symbol Gene Description ACVR1B Activin a Receptor, Type IB
Table S1. Kinase clones included in human kinase cDNA library for yeast two-hybrid screening Gene Symbol Gene Description ACVR1B activin A receptor, type IB ADCK2 aarF domain containing kinase 2 ADCK4 aarF domain containing kinase 4 AGK multiple substrate lipid kinase;MULK AK1 adenylate kinase 1 AK3 adenylate kinase 3 like 1 AK3L1 adenylate kinase 3 ALDH18A1 aldehyde dehydrogenase 18 family, member A1;ALDH18A1 ALK anaplastic lymphoma kinase (Ki-1) ALPK1 alpha-kinase 1 ALPK2 alpha-kinase 2 AMHR2 anti-Mullerian hormone receptor, type II ARAF v-raf murine sarcoma 3611 viral oncogene homolog 1 ARSG arylsulfatase G;ARSG AURKB aurora kinase B AURKC aurora kinase C BCKDK branched chain alpha-ketoacid dehydrogenase kinase BMPR1A bone morphogenetic protein receptor, type IA BMPR2 bone morphogenetic protein receptor, type II (serine/threonine kinase) BRAF v-raf murine sarcoma viral oncogene homolog B1 BRD3 bromodomain containing 3 BRD4 bromodomain containing 4 BTK Bruton agammaglobulinemia tyrosine kinase BUB1 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) BUB1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) C9orf98 chromosome 9 open reading frame 98;C9orf98 CABC1 chaperone, ABC1 activity of bc1 complex like (S. pombe) CALM1 calmodulin 1 (phosphorylase kinase, delta) CALM2 calmodulin 2 (phosphorylase kinase, delta) CALM3 calmodulin 3 (phosphorylase kinase, delta) CAMK1 calcium/calmodulin-dependent protein kinase I CAMK2A calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha CAMK2B calcium/calmodulin-dependent
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Genome-Wide DNA Methylation Analysis of KRAS Mutant Cell Lines Ben Yi Tew1,5, Joel K
www.nature.com/scientificreports OPEN Genome-wide DNA methylation analysis of KRAS mutant cell lines Ben Yi Tew1,5, Joel K. Durand2,5, Kirsten L. Bryant2, Tikvah K. Hayes2, Sen Peng3, Nhan L. Tran4, Gerald C. Gooden1, David N. Buckley1, Channing J. Der2, Albert S. Baldwin2 ✉ & Bodour Salhia1 ✉ Oncogenic RAS mutations are associated with DNA methylation changes that alter gene expression to drive cancer. Recent studies suggest that DNA methylation changes may be stochastic in nature, while other groups propose distinct signaling pathways responsible for aberrant methylation. Better understanding of DNA methylation events associated with oncogenic KRAS expression could enhance therapeutic approaches. Here we analyzed the basal CpG methylation of 11 KRAS-mutant and dependent pancreatic cancer cell lines and observed strikingly similar methylation patterns. KRAS knockdown resulted in unique methylation changes with limited overlap between each cell line. In KRAS-mutant Pa16C pancreatic cancer cells, while KRAS knockdown resulted in over 8,000 diferentially methylated (DM) CpGs, treatment with the ERK1/2-selective inhibitor SCH772984 showed less than 40 DM CpGs, suggesting that ERK is not a broadly active driver of KRAS-associated DNA methylation. KRAS G12V overexpression in an isogenic lung model reveals >50,600 DM CpGs compared to non-transformed controls. In lung and pancreatic cells, gene ontology analyses of DM promoters show an enrichment for genes involved in diferentiation and development. Taken all together, KRAS-mediated DNA methylation are stochastic and independent of canonical downstream efector signaling. These epigenetically altered genes associated with KRAS expression could represent potential therapeutic targets in KRAS-driven cancer. Activating KRAS mutations can be found in nearly 25 percent of all cancers1.
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And Rasopathies-Associated SHP2 and BRAF Mutations
Functional Characterization of Cancer- and RASopathies-associated SHP2 and BRAF Mutations Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) im Fach Biologie/ Molekularbiologie eingereicht an der Lebenswissenschaftlichen Fakultät der Humboldt–Universität zu Berlin von M. Sc. Paula Andrea Medina-Pérez Präsident der Humboldt–Universität zu Berlin Prof. Dr. Jan–Hendrik Olbertz Dekan der Lebenswissenschaftlichen Fakultät Prof. Dr. Richard Lucius Gutachter: 1. Prof. Dr. Reinhold Schäfer 2. Prof. Dr. Hanspeter Herzel 3. Prof. Dr. Holger Sültmann Tag der mündlichen Prüfung: 12.03.2015 Abstract Deregulation of the Ras/MAPK signaling is implicated in a wide variety of human diseases, including developmental disorders and cancer. In the last years, a group of developmental disorders, characterized by an overlapping phenotype in patients, was clustered under the term RASopathies. These disorders result from germline mutations in genes encoding key components of the Ras/MAPK signaling cascade. Although the incidence of solid tumors in patients suffering from these disorders is rather low, reports on different forms of leukemia have considerably increased. In this work, a group of mutations in the genes SHP2/PTPN11 and BRAF, both key regulators of the MAPK signaling pathway and implicated in RASopathies and cancer, were selected for expression in well-established cell systems for a comprehensive molecular and phenotypic characterization using high-throughput approaches and functional assays. Synthetic cDNA sequences carrying the SHP2 mutations T42A, E76D, I282V (Noonan syndrome-associated), E76G, E76K, E139D (Noonan- and leukemia-associated), T468M (LEOPARD syndrome -associated) and the BRAF mutations Q257R, S467A, L485F and K499E (cardio-facio-cutaneous syndrome-associated) were shuttled into the modified lentivi- ral vector pCDH-EF1-IRES-GFP.
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Unit Name Gene Name Forward Sequence Reverse Sequence
SupplTable2.xls Unit name Gene name Forward sequence Reverse sequence AKAP13_0 AKAP13 CAGTGATGTGTCTCTCTCC GTTCTCTCTGGTCTGACTC APAF1_0 APAF1 GGACCCTCAAGAGGATATG GAAAGTACTGTACCCTGGTG ARID4B_0 ARID4B GAGGAGTACTGTAGATCAGC TGGCATCAGTGAGATCCAG ASC_0 ASC CTTCTACCTGGAGACCTAC GTAGGACTGGGACTCCCTTA AXIN1_0 AXIN1 GAGGCTACTCAGAGAGTGTT CAGAAGTAGTACGCCACAAC AXL_0 AXL CCCCTGAGAACATTAGTGCT AGAGCCAAGATGAGGACACA AXUD1_0 AXUD1 GTCCGGCACCACCATGACT GATGTGAAGCTACAGCCAG BAX_0 BAX GAGCAGATCATGAAGACAGG GTCCCAAAGTAGGAGAGGA BCL2L11_0 BCL2L11 TACCTCCCTACAGACAGAG CCTCCTTGCATAGTAAGCGT BCL2L12_0 BCL2L12 TCCTAGCTGCCTTCCTTAG GTATGGCTTCCTTCTCTGTC BCL2L1_0 BCL2L1 ATGGCAGCAGTAAAGCAAGCG TCATTTCCGACTGAAGAGTGA BCLAF1_0 BCLAF1 GTACCCTGAGGAAGCATAC AGTACCACGACCTCTTCCT BIRC5_0 BIRC5 CCACCGCATCTCTACATTCA CTGGTGCCACTTTCAAGACA BNIP1_0 BNIP1 CCGGATCTGTAACCAAGAG CTTAAGAGATCTCCTCCCTG BNIP1_1 BNIP1 CAACTCTCCAACTACACCTG CTCTCAGTGATGGTACTGG BRAF_0 BRAF GAGAGGTCTAATCCCAGAGT CACGTTAGTTAGTGAGCCAG BRCA1_0 BRCA1 CTAACAGCTACCCTTCCATC ACCACATCTCCTCTGACTTC BRCA1_1 BRCA1 GTCTCAGTGTCCAACTCTCT GTCACTCTGAGAGGATAGC C11orf17_0 C11orf17 CTCTAGAAGTGCTGGAGAG GACCATTCCCTATGTCCAAG C11orf17_1 C11orf17 GTTATCACAGAGGCGAGTC CAACTGCTACCACATGAGTC CARD10_0 CARD10 GAGTTCTACTACCCCGAAC CTCAGAGACAAGGTCCACAT CARD10_1 CARD10 GTGGACCTTGTCTCTGAGC GTACAGGTCACAGTCCTTC CASP10_0 CASP10 AGACTCGCTTCCCAAAACTG ATCTGCTTCGATGGATACGG CASP1_0 CASP1 AGGTCCTGAAGGAGAAGAG CCTCCACATCACAGGAACA CASP4_0 CASP4 GGGTCATGGCAGACTCTAT CTATAGTCCAGACCCTCAAG CASP6_0 CASP6 ATGAGCTCGGCCTCGGGG GGCTGCATCCACCTCAGTTA CASP7_0 CASP7 GACCGAGTGCCTACATATC GCTCCTCCACGAGTAATAG
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Supporting Information
Supporting Information Faltermeier et al. 10.1073/pnas.1521674112 A. FU-R1-R2-V5-SV40-BlasƟ-CGW UbiquiƟn-C CMV 5’ LTR R1 Kinase R2 V5 SV40 BlastiR 3’ LTR promoter promoter GFP B. Kinase (kDa) 210 CMV GFP WPR 105 promot E er 78 49 38 28 V5 293t cells Fig. S1. Lentivirus-mediated overexpression of V5-tagged kinases. (A) Full-length kinases were cloned into the FU-R1-R2-V5-SV40-Blasti-CGW lentiviral vector shown. R1 and R2 represent recombination sites required for recombination-based Gateway cloning. LTR, Long-terminal repeat. (B) Western blot showing expression of selected kinases in 293t cells detected by a V5 antibody. The molecular mass of each kinase is indicated in parentheses. A. vector SrcY529F 3 Cap8 cells 2 weeks 2 x107 Tail vein Y529F Src injecƟon SCID 1 Luciferase reporter BLI Radiance (p/sec/cm3/sr) B. Lungs from mice injected with Cap8-SrcY529F cells Mouse 1 Mouse 2 Fig. S2. SrcY529F promotes lung colonization when overexpressed in murine prostate cells. (A) Experimental design to demonstrate that expression of mu- tationally activated kinase SrcY529F in Cap8 cells promotes lung colonization. (B) Bright-field images of lungs removed from mice 3 wk after being injected with Cap8-SrcY529F cells. (Scale bars, 5 mm.) Faltermeier et al. www.pnas.org/cgi/content/short/1521674112 1of5 A. Lungs-NTRK2 210 105 NTRK2 78 49 38 V5 293t cells Metastases Ɵssue Lungs-EGFR 210 EGFR 105 78 49 38 V5 293t cells Metastases Ɵssues Lungs-Her2 210 105 HER2 78 49 38 V5 293t cells Metastases Ɵssues B.
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Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM 014911.2 KIAA1048, Dkfzp686k16132 AP2 Associated Kinase 1
Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM_014911.2 KIAA1048, DKFZp686K16132 AP2 associated kinase 1 (AAK1) AATK NM_001080395.2 AATYK, AATYK1, KIAA0641, LMR1, LMTK1, p35BP apoptosis-associated tyrosine kinase (AATK) ABL1 NM_007313.2 ABL, JTK7, c-ABL, p150 v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1) ABL2 NM_007314.3 ABLL, ARG v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (ABL2) ACVR1 NM_001105.2 ACVRLK2, SKR1, ALK2, ACVR1A activin A receptor ACVR1B NM_004302.3 ACVRLK4, ALK4, SKR2, ActRIB activin A receptor, type IB (ACVR1B) ACVR1C NM_145259.2 ACVRLK7, ALK7 activin A receptor, type IC (ACVR1C) ACVR2A NM_001616.3 ACVR2, ACTRII activin A receptor ACVR2B NM_001106.2 ActR-IIB activin A receptor ACVRL1 NM_000020.1 ACVRLK1, ORW2, HHT2, ALK1, HHT activin A receptor type II-like 1 (ACVRL1) ADCK1 NM_020421.2 FLJ39600 aarF domain containing kinase 1 (ADCK1) ADCK2 NM_052853.3 MGC20727 aarF domain containing kinase 2 (ADCK2) ADCK3 NM_020247.3 CABC1, COQ8, SCAR9 chaperone, ABC1 activity of bc1 complex like (S. pombe) (CABC1) ADCK4 NM_024876.3 aarF domain containing kinase 4 (ADCK4) ADCK5 NM_174922.3 FLJ35454 aarF domain containing kinase 5 (ADCK5) ADRBK1 NM_001619.2 GRK2, BARK1 adrenergic, beta, receptor kinase 1 (ADRBK1) ADRBK2 NM_005160.2 GRK3, BARK2 adrenergic, beta, receptor kinase 2 (ADRBK2) AKT1 NM_001014431.1 RAC, PKB, PRKBA, AKT v-akt murine thymoma viral oncogene homolog 1 (AKT1) AKT2 NM_001626.2 v-akt murine thymoma viral oncogene homolog 2 (AKT2) AKT3 NM_181690.1
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Koch Shrna Gene Webpage
Symbol SEPT9 ADAM30 AEN AMBP ARHGEF12 ATG16L2 BCAS3 A1CF ADAM32 AFF3 AMBRA1 ARHGEF17 ATG2A BCKDK AAK1 ADAM33 AGAP2 AMHR2 ARHGEF2 ATG3 BCL10 AATK ADAM7 AGER AMPH ARHGEF4 ATG4B BCL11A ABCA1 ADAM8 AGK ANAPC2 ARHGEF6 ATG4C BCL11B ABCA3 ADAM9 AGL ANG ARHGEF7 ATG4D BCL2 ABCB1 ADAMDEC1 AGPAT9 ANGPT2 ARID1A ATG5 BCL2L1 ABCB4 ADAMTS1 AGR3 ANGPTL4 ARID1B ATG7 BCL2L11 ABCC1 ADAMTS10 AHR ANKK1 ARID2 ATM BCL2L2 ABCC10 ADAMTS12 AIMP2 ANKRD30A ARID3A ATMIN BCL3 ABCC2 ADAMTS13 AIP ANO1 ARID3B ATP1B3 BCL6 ABCG2 ADAMTS14 AJAP1 ANXA1 ARID4B ATP2B4 BCL7A ABI1 ADAMTS15 AK1 ANXA2 ARID5A ATP7A BCL9 ABL1 ADAMTS16 AK2 ANXA6 ARID5B ATP7B BCR ABL2 ADAMTS17 AK3 ANXA7 ARL11 ATR BECN1 ACIN1 ADAMTS18 AK4 APAF1 ARNT ATRX BFAR ACP1 ADAMTS19 AK5 APC ARSB ATXN1 BIK ACPP ADAMTS2 AK7 APCDD1 ARSG ATXN2 BIN1 ACSL4 ADAMTS20 AK8 APEX1 ASAP1 AURKA BIN2 ACTN1 ADAMTS3 AKAP1 APOBEC1 ASAP3 AURKB BIRC2 ACVR1 ADAMTS4 AKAP13 APOBEC2 ASB15 AURKC BIRC3 ACVR1B ADAMTS5 AKAP3 APOBEC3G ASCC1 AXIN1 BIRC5 ACVR1C ADAMTS7 AKAP8L AQP1 ASCC3 AXIN2 BIRC7 ACVR2A ADAMTS8 AKR1B10 AQP5 ASCL1 AXL BLCAP ACVR2B ADAMTS9 AKR1C1 AQP7 ASCL2 AZGP1 BLK ACVRL1 ADAR AKR1C3 AR ASF1A BACE1 BLM AD026 ADARB1 AKT1 ARAF ASH1L BAD BMI1 ADAM10 ADARB2 AKT2 AREG ASH2L BAG1 BMP2 ADAM11 ADAT2 AKT3 ARF1 ASNS BAG4 BMP2K ADAM12 ADCK1 ALCAM ARF4 ASPH BANF1 BMP2KL ADAM15 ADCK2 ALDH18A1 ARF5 ASPSCR1 BAP1 BMPR1A ADAM17 ADCK3 ALK ARF6 ASS1 BARD1 BMPR1B ADAM18 ADCK4 ALKBH2 ARHGAP12 ASTE1 BAX BMPR2 ADAM19 ADCK5 ALKBH3 ARHGAP22 ASXL1 BAZ1A BMX ADAM2 ADCY6 ALKBH8 ARHGAP25 ATF1 BAZ1B BNIP3 ADAM20 ADK ALOX15 ARHGAP26 ATF2 BAZ2A BPTF ADAM21
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HPFM3 Panorama™ Human Kinase V1 Array
Panorama™ Human Kinase v1 Array Technical Bulletin Catalog Number HPFM3 Panorama™ Human Kinase v1 Array Table of Contents Introduction ......................................................................................................................................... 1 Panorama Array Technology ................................................................................................................. 1 Kit Contents ........................................................................................................................................ 1 Storage Conditions .............................................................................................................................. 2 General Recommendations .................................................................................................................. 2 Kit Components ................................................................................................................................... 3 Protein Arrays .................................................................................................................................................. 3 Anti-phosphotyrosine-Cy5................................................................................................................................ 4 Anti-c-Myc-Cy3 ................................................................................................................................................ 4 Control Assays ....................................................................................................................................
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Table S1. 103 Ferroptosis-Related Genes Retrieved from the Genecards
Table S1. 103 ferroptosis-related genes retrieved from the GeneCards. Gene Symbol Description Category GPX4 Glutathione Peroxidase 4 Protein Coding AIFM2 Apoptosis Inducing Factor Mitochondria Associated 2 Protein Coding TP53 Tumor Protein P53 Protein Coding ACSL4 Acyl-CoA Synthetase Long Chain Family Member 4 Protein Coding SLC7A11 Solute Carrier Family 7 Member 11 Protein Coding VDAC2 Voltage Dependent Anion Channel 2 Protein Coding VDAC3 Voltage Dependent Anion Channel 3 Protein Coding ATG5 Autophagy Related 5 Protein Coding ATG7 Autophagy Related 7 Protein Coding NCOA4 Nuclear Receptor Coactivator 4 Protein Coding HMOX1 Heme Oxygenase 1 Protein Coding SLC3A2 Solute Carrier Family 3 Member 2 Protein Coding ALOX15 Arachidonate 15-Lipoxygenase Protein Coding BECN1 Beclin 1 Protein Coding PRKAA1 Protein Kinase AMP-Activated Catalytic Subunit Alpha 1 Protein Coding SAT1 Spermidine/Spermine N1-Acetyltransferase 1 Protein Coding NF2 Neurofibromin 2 Protein Coding YAP1 Yes1 Associated Transcriptional Regulator Protein Coding FTH1 Ferritin Heavy Chain 1 Protein Coding TF Transferrin Protein Coding TFRC Transferrin Receptor Protein Coding FTL Ferritin Light Chain Protein Coding CYBB Cytochrome B-245 Beta Chain Protein Coding GSS Glutathione Synthetase Protein Coding CP Ceruloplasmin Protein Coding PRNP Prion Protein Protein Coding SLC11A2 Solute Carrier Family 11 Member 2 Protein Coding SLC40A1 Solute Carrier Family 40 Member 1 Protein Coding STEAP3 STEAP3 Metalloreductase Protein Coding ACSL1 Acyl-CoA Synthetase Long Chain Family Member 1 Protein
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Targeted Epigenetic Remodeling of the Cdk5 Gene in Nucleus Accumbens Regulates Cocaine- and Stress-Evoked Behavior
4690 • The Journal of Neuroscience, April 27, 2016 • 36(17):4690–4697 Cellular/Molecular Targeted Epigenetic Remodeling of the Cdk5 Gene in Nucleus Accumbens Regulates Cocaine- and Stress-Evoked Behavior X Elizabeth A. Heller,1* Peter J. Hamilton,1* Dominika D. Burek,1 XSonia I. Lombroso,1 Catherine J. Pen˜a,1 Rachael L. Neve,2 and XEric J. Nestler1 1Fishberg Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, and 2McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 Recent studies have implicated epigenetic remodeling in brain reward regions following psychostimulant or stress exposure. It has only recently become possible to target a given type of epigenetic remodeling to a single gene of interest, and to probe the functional relevance of such regulation to neuropsychiatric disease. We sought to examine the role of histone modifications at the murine Cdk5 (cyclin- dependent kinase 5) locus, given growing evidence of Cdk5 expression in nucleus accumbens (NAc) influencing reward-related behav- iors. Viral-mediated delivery of engineered zinc finger proteins (ZFP) targeted histone H3 lysine 9/14 acetylation (H3K9/14ac), a transcriptionally active mark, or histone H3 lysine 9 dimethylation (H3K9me2), which is associated with transcriptional repression, specifically to the Cdk5 locus in NAc in vivo. We found that Cdk5-ZFP transcription factors are sufficient to bidirectionally regulate Cdk5 gene expression via enrichment of their respective histone modifications. We examined the behavioral consequences of this epigenetic remodeling and found that Cdk5-targeted H3K9/14ac increased cocaine-induced locomotor behavior, as well as resilience to social stress.
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Kinome Expression Profiling to Target New Therapeutic Avenues in Multiple Myeloma
Plasma Cell DIsorders SUPPLEMENTARY APPENDIX Kinome expression profiling to target new therapeutic avenues in multiple myeloma Hugues de Boussac, 1 Angélique Bruyer, 1 Michel Jourdan, 1 Anke Maes, 2 Nicolas Robert, 3 Claire Gourzones, 1 Laure Vincent, 4 Anja Seckinger, 5,6 Guillaume Cartron, 4,7,8 Dirk Hose, 5,6 Elke De Bruyne, 2 Alboukadel Kassambara, 1 Philippe Pasero 1 and Jérôme Moreaux 1,3,8 1IGH, CNRS, Université de Montpellier, Montpellier, France; 2Department of Hematology and Immunology, Myeloma Center Brussels, Vrije Universiteit Brussel, Brussels, Belgium; 3CHU Montpellier, Laboratory for Monitoring Innovative Therapies, Department of Biologi - cal Hematology, Montpellier, France; 4CHU Montpellier, Department of Clinical Hematology, Montpellier, France; 5Medizinische Klinik und Poliklinik V, Universitätsklinikum Heidelberg, Heidelberg, Germany; 6Nationales Centrum für Tumorerkrankungen, Heidelberg , Ger - many; 7Université de Montpellier, UMR CNRS 5235, Montpellier, France and 8 Université de Montpellier, UFR de Médecine, Montpel - lier, France ©2020 Ferrata Storti Foundation. This is an open-access paper. doi:10.3324/haematol. 2018.208306 Received: October 5, 2018. Accepted: July 5, 2019. Pre-published: July 9, 2019. Correspondence: JEROME MOREAUX - [email protected] Supplementary experiment procedures Kinome Index A list of 661 genes of kinases or kinases related have been extracted from literature9, and challenged in the HM cohort for OS prognostic values The prognostic value of each of the genes was computed using maximally selected rank test from R package MaxStat. After Benjamini Hochberg multiple testing correction a list of 104 significant prognostic genes has been extracted. This second list has then been challenged for similar prognosis value in the UAMS-TT2 validation cohort.
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FASTK Antibody
Product Datasheet FASTK Antibody Catalog No: #35737 Orders: [email protected] Description Support: [email protected] Product Name FASTK Antibody Host Species Rabbit Clonality Polyclonal Purification Antigen affinity purification. Applications IHC Species Reactivity Hu Specificity The antibody detects endogenous levels of total FASTK protein. Immunogen Type Recombinant Protein Immunogen Description Fusion protein corresponding to residues near the C terminal of human fas-activated serine/threonine kinase Target Name FASTK Other Names FAST Accession No. Swiss-Prot#: Q14296NCBI Gene ID: 10922Gene Accssion: BC000377 Concentration 1.3mg/ml Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. Storage Store at -20°C Application Details Immunohistochemistry: 1:50-1:200 Images Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #35737 at dilution 1/30. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemical analysis of paraffin-embedded Human brain tissue using #35737 at dilution 1/30. Background The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. Note: This product is for in vitro research use only and is not intended for use in humans or animals. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2.
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