cazymes 2021 2 NZYTech 2021 awesome news andpromotions. Subscribe our newsletter to receive Contact [email protected] your localsalesrepresentativeformoreinformation. www V Ne F ol isit theOnl w l o sl w us: et t er .nz ine S t or yt e hc yu u-odt balance and track your latest orders. up-to-date your Store, any check Online our buy from can you With NZYWallet research. your on focus to need you flexibility the oers that account prepaid a is NZYWallet NZYW ech.com al l et Inst done! to Checkout, select quote andit’s add your products to Cart, proceed Do you needanurgent quote? Just ant aneous Quot es awesome news andpromotions. Subscribe our newsletter to receive Contact [email protected] your localsalesrepresentativeformoreinformation. www V F Ne ol isit theOnl w l o sl w us: et t er .nz ine S t or yt e hc yu u-odt balance and track your latest orders. up-to-date your Store, any check Online our buy from can product you With NZYWallet research. your on focus to need you flexibility the oers that account prepaid a is NZYWallet NZYW ech.com al l et Inst done! to Checkout, select quote andit’s add your products to Cart, proceed Do you needanurgent quote? Just ant aneous Quot es

3 cazymes 4 NZYTech 2021 aatslNaeyhxsmn phosphorylases Galactosyl-N-acetylhexosamine β DGlcoy-,--hmoe phosphorylases-D-Galactosyl-1,4-L-rhamnose Phospho-β Acetylgalactosaminidases Phospho-β Acetylglucosaminidases Arabinopyranosidases Arabinofuranosidases Fructofuranosidases Glucuronoxylanases Cellobiohydrolases Glucosaminidases Arabinoxylanases Galacturonidases GLYCOSIDE α β Cellodextrinases - -Galactosidases -Galactosidases Amylomaltases Glucansucrases Glucuronidases α β Laminarinases Carrageenases Levansucrases - -Glucosidases -Glucosidases Arabinanases Galactanases Chitosanases Dextranases Fructanases Fucosidases Lichenases Levanases Agarases Inulases OVERVIEW 10 8

@ a glance

5 cazymes 6 NZYTech 2021 39 45 Poly- CARBOHYDRATE ESTERASES Δ β α β α Xanthan lyases Ulvan lyases Rhamnogalacturonan lyases Pectin lyases Pectate lyases Oligogalacturonate lyases Hyaluronate lyases Heparin lyases Chondroitin lyases Alginate lyases Pectin methylesterases Pectin acetyl esterases Glucuronyl esterases Feruloyl esterases Diacetylchitobiose deacetylases Acetylglucosamine deacetylases Acetyl xylan esterases Other Activities Xylosidases Xyloglucanases Trehalases Sialidases Rhamnogalacturonases Porphyranases Peptidoglycan lytic exotransglycosylases Oligosaccharide reducing-end xylanases Mannosylglucose phosphorylases -Mannosidases -Mannanases -Mannosidases -Mannanases -4,5-Unsaturated α -guluronate lyases β -glucuronyl hydrolases Lytic polysaccharide monooxygenases MINI-CELLULOSOMES & O

CARBOHYDRATE-BINDING MODULES Cellobiose dehydrogenase Mini-cellulosomes Xylose TERMS &CONDITIONS AUXILIARY ACTIVITIES GFP-CBM Laccases zz-CBM CBMs ther 50 59 52 61

@ a glance

7 cazymes 8 NZYTech 2021 A plates NZYTech CAZymes starts at1.999€. this catalogue in plates. Contact us incase you want to receive the enzymes inplates. Price perplate containing 50 µLof96enzymes In case you want to test alarge numberofenzymes simultaneously, NZYTech can provide afraction or even all theenzymes listed in produced andpurified to meetthesame quality standards. of theenzymes listed inthiscatalogue, upto the500gram scale, please contact us. You will have access to large quantities ofthe NZYTech highly pure CAZymes are provided inthemgscale (1vialat149€ or3vials at329€). Ifyou require larger quantities ofany Specifies theCAZyme catalogue number. Catalogue Number from humanblood group AandABmucinglycoproteins. N-acetyl-β-D-galactosaminides. β-N-Acetylgalactosaminidases are enzymes thatparticipate inthehydrolysis ofterminal non-reducing N-acetyl-D-galactosamine residues in HOW TO USE THIS CATALOG OVERVIEW CAZymes. In thiscatalogue CAZymes are grouped by enzyme activity. There are > 80 different enzyme categories to list >1000 Catalogue Number CZ0515 cetylgalactosaminidases β-N-Acetylgalactosaminidase 123A Enzyme molecular architecture. Refers to theCAZy family andenzyme CAZ Refers to theenzyme namefollowing the suggested nomenclature for CAZymes as described by Henrissat B. etal. (1998) FEBS Lett 425:352-354. Enzyme y Architecture α-N-Acetylgalactosaminidases are enzymes that cleave non-reducing 1,3-α-N-acetylgalactosamine residues Source Organism Refers to thehost organism from whichthe are enzyme was isolated. All enzymes inthis catalogue have arecombinant origin Optima temperature andpHfor enzyme activity. Temperature andpH and are produced eitherinPichiapas - toris orEscherichia coli. available up Source Organism Paenibacillus sp. ue? Architecture CAZ GH123 to y the EC Number The (EC number) is a numerical classification scheme for enzymes, based onthechemical reactions they catalyze. mL at149€ andthree vials at329€. provided pervial. NZYTech sells onevialwith1 Specifies thenumberofmgpurifiedCAZyme mg/vial 3.2.1.53 EC gram Optimum pH 6.0 scale Temperature (ºC) Optimum 37 and mg/ vial 0.25 in

that are more suited to your application and/or experiment. instructions that accompany each enzyme. Having available well described enzymes allows you selecting the catalysts ence of the paper describing the properties of the different enzymes is provided at www.nzytech.com or in the technical The majority ofNZYTech CAZymes display well-defined enzyme properties thatare described intheliterature. Refer biochemical properties described inliterature 4. NZYTech CA Zymes are throughly characterized andtheir tivity upto 5years after production (Panel B). Thus, itisimportant to follow thestorage guidelines provided for each enzyme. lecular integrity (Panel A) andactivity (Panel B)between batches while stability isreflected by retention offull enzyme ac March 2018for molecular integrity, protein purity andenzyme activity. The data reveals thatthere isminimalvariation inmo- 4 batches ofXylanase 11Afrom thermocellum (CZ0003), produced in2013, 2014, 2015and2016, were tested in NZYTech devotes significant efforts to minimize batch to batch variation andimprove enzyme stability. IntheFigure below 3. Reduced batch- nzytech.com). CZ0633 andCZ0641. Purification level ofall CAZymes listed inthiscatalogue may befound atNZYTech website (www. protein purity. Asanexample, thefigure below provides anSDS-PAGE analysis ofCAZymes CZ0604, CZ0580, CZ0621, NZYTech CAZymes are purifiedusingacomprehensive list ofchromatography protocols to achieve thehighestlevel of 2. Ultra covering >200 CAZy families (www.cazy.com) and>150 EC number activities(www.enzyme..org). produced andpurifiedfrom Pichiapastoris orEscherichia coli. Inthiscatalogue you will findmore than1000CAZymes, portfolio ofresearch enzymes to foster science andindustrialprogress. All enzymes have arecombinant originandare NZYTech R&D department, individually or in collaboration with the CAZy community, is building a large and diverse 1. Alarge anddiverse portf research tools f NZYTech CAZymes: highly pure, stable andoptimized -pure enzymes f to-batch variation andlong storage stability or science andindustry or avariety ofapplications olio ofrecombinant enzymes

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9 cazymes 10 NZYTech 2021 glycoside hydrolases β α Glucosaminidase Glucansucrase G G β Phospho-β β α G Fructofuranosidase Fructanase De Cellode Cellobiohydrolase C Arabinox Arabinop Arabinofuranosidase Arabinanase Amylomaltase Agaras A A -Glucosidase -D-G -G -Glucosidase -G arrageenase alacturonidase alactos alactanase ce ce xtranase alactosidase alactosidase tylglucosaminidase tylgalactosaminidase alactos e xtrinase s s yl ylanase yranosidase

s

s -G

s

-N- s s

alactosidase s

s s yl

s

s

ace s s

-1,4-L-rhamnose phosphor

s

s s

s s s

s tylhe

s

s s s

xosamine s

s s

phosphor ylase * Structure ofCellulase 5Bfrom Bacillus halodurans (PDB4V2X), #CZ0476, pg17 ylase s

s

Lev Lev Laminarinase Inulase Hy He Glucuronox Glucuronidase Phospho-β Ly Peptidogl Oligosaccharide rducing-end x Mannos β α β α Pol Xylanas e Δ Sialidase Rhamnogalacturonase P Porphyranase Xylosidase Xyloglucanase O - -Mannanase -Mannosidase -Mannanase -4,5-Unsaturated ullulanase ther A so aluronidase xosaminidase ansucrase anase y zyme galacturonase s ctivitie ylglucose phosphor

s s s s y s

-Glucosidase s

can l s

ylanase s s s s

s

s s s s s s

s

s

ytic e

β -glucuronyl hdrolase s s

xotransgl

s s

ylase y ylanase cos s

ylase s

s

s

dues from oligosaccharides andpeptidoglycans. β-N-Acetylglucosaminidases are highly specific glycoside hydrolases that catalyze the ofβ-N-acetylglucosamine resi- A 1,3-α-N-acetylgalactosamine residues from humanblood group AandABmucinglycoproteins. tosamine residues in N-acetyl-β-D-galactosaminides. β-N-Acetylgalactosaminidases are enzymes thatparticipate inthehydrolysis ofterminal non-reducing N-acetyl-D-galac A Catalogue Catalogue Number Number CZ0840 CZ0969 CZ0830 CZ0828 CZ0428 CZ0829 CZ0955 CZ0893 CZ0824 CZ0798 CZ0835 CZ0543 CZ0229 CZ0832 CZ0879 CZ0833 CZ0976 CZ0106 CZ0837 CZ0572 CZ0891 CZ0821 CZ0831 CZ0515 cetylglucosaminidases cetylgalactosaminidases Endo-α-N-acetylgalactosaminidase 101A Endo-α-N-acetylgalactosaminidase 101A Endo-N-acetylglucosaminidase 111A α-N-Acetylgalactosaminidase 109A α-N-Acetylgalactosaminidase 129A β-N-Acetylgalactosaminidase 123A β-N-Acetylglucosaminidase 84A β-Acetylglucosaminidase 85A β-Acetylglucosaminidase 73A β-Acetylglucosaminidase 73A β-Acetylglucosaminidase 73A β-Acetylglucosaminidase 73A β-Acetylglucosaminidase 73A β-Acetylglucosaminidase 73B β-Acetylglucosaminidase 18A β-Acetylglucosaminidase 18A β-Acetylglucosaminidase 18A β-Acetylglucosaminidase 18A β-Acetylglucosaminidase 18A β-Acetylglucosaminidase 18A β-Acetylglucosaminidase 18A β-Acetylglucosaminidase 18A β-Acetylglucosaminidase 18B β-Acetylglucosaminidase 18B Keratan sulfate Enzyme Enzyme Elizabethkingia meningoseptica Elizabethkingia meningoseptica Elizabethkingia meningoseptica Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Capnocytophaga canimorsus Streptococcus pneumoniae Streptococcus pneumoniae Lactobacillus plantarum Bifidobacterium bifidum Bifidobacterium longum Clostridium perfringens Streptomyces plicatus Enterococcus faecalis Bacillus megaterium Clostridium difficile Flavobacterium sp. Source Organism Source Organism Lactococcus lactis Lactococcus lactis Bacillus anthracis Bacillus circulans Paenibacillus sp. α-N-Acetylgalactosaminidases are enzymes that cleave non-reducing GH84-CBM32 Architecture CBM50-GH18 CBM50-GH18 Architecture CBM50- CBM50- CAZ GH85 GH109 GH111 GH73 GH73 GH73 GH73 GH73 GH129 GH73 GH18 GH18 GH18 GH18 GH123 GH18 GH18 GH18 GH18 GH101 GH101 CAZ y y 3.2.1.169 3.2.1.96 3.2.1.96 3.2.1.96 3.2.1.96 3.2.1.96 3.2.1.96 3.2.1.96 3.2.1.96 3.2.1.96 3.2.1.49 3.2.1.49 3.2.1.53 3.2.1.97 3.2.1.97 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- EC EC Optimum Optimum Optimum Optimum 7,0-8,0 6,0-8,0 4,0-5,0 5,0-6,0 7,0-8,0 6,0-7,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 8,0 6,0 6,0 6,0 6,0 8,0 6,8 6,8 5,0 pH pH 7,0 7,5 7,5 Temperature (ºC) Temperature (ºC) Optimum Optimum 60 50 30 26 55 35 35 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 0,25 0,25 0,25 0,25 0,25 0,25 0,25 vial vial 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 -

11 cazymes 12 NZYTech 2021 animals, plants andmicrobes. the importance ofstarch asacarbon and energy source, amylases are highly ubiquitous enzymes innature beingproduced by Amylases are enzymes that catalyze the hydrolysis of starch and glycogen into sugars by acting on Amylases Agarases are thefirstenzymes intheagar catabolic pathway anddegrade bothα Agarases Catalogue Catalogue Number Number CZ0549 CZ0394 CZ0230 CZ0407 CZ0562 CZ0538 CZ0263 CZ0534 CZ0453 CZ0234 CZ0750 CZ0764 CZ0749 CZ0297 CZ0738 CZ0736 CZ0739 CZ0537 CZ0733 CZ0743 CZ0165 CZ0747 CZ0152 CZ0517 CZ0741 CZ0751 CZ0317 α-Neoagarobiose 117A α-Neoagarobiose hydrolase 117A α-Neoagarobiose hydrolase 117A α-Neoagarobiose hydrolase 117B α-Neoagarobiose hydrolase 117C Maltogenic α-amylase 13A Maltogenic α-amylase 13A Glucoamylase 97A β- 118A β-Agarase 86A β-Agarase 50D β-Agarase 50A β-Agarase 50A β-Agarase 50A β-Amylase 14A α-Agarase 117A β-Agarase 16D β-Agarase 16A β-Agarase 16A β-Agarase 16A β-Agarase 16B β-Agarase 16B Amylase 126A Amylase 57C Amylase 13A Amylase 13A Amylase 13A Enzyme Enzyme Bacteroides thetaiotaomicron Microbulbifer thermotolerans Saccharophagus degradans Saccharophagus degradans Saccharophagus degradans Thermotoga neapolitana Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Clostridium perfringens Streptococcus mutans Thermotoga maritima Janthinobacterium sp. Bacteroides uniformis Bacteroides uniformis Bacteroides uniformis Bacillus licheniformis Bacteroides plebeius Bacteroides plebeius Bacteroides plebeius Lactobacillus gasseri Bacteroides ovatus Source Organism Source Organism Bacillus circulans Agarivorans sp. Escherichia coli Architecture Architecture GH16-CBM6 GH126 GH118 GH86 GH50 GH50 GH50 GH50 CAZ CAZ GH117 GH117 GH117 GH117 GH117 GH117 GH97 GH57 GH16 GH16 GH16 GH16 GH16 GH14 GH13 GH13 GH13 GH13 GH13 y y orβ 3.2.1.133 3.2.1.133 3.2.1.81 3.2.1.81 3.2.1.81 3.2.1.81 3.2.1.81 3.2.1.81 linkages inagarose. 3.2.1.81 3.2.1.81 3.2.1.81 3.2.1.81 3.2.1.81 3.2.1.81 3.2.1.3 3.2.1.2 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.1 3.2.1.1 3.2.1.1 3.2.1.1 EC EC Optimum Optimum Optimum 4,0-12,0 7,0-8,0 6,5-7,5 6,5-7,5 6,5-7,5 8,0 8,0 8,0 6,0 8,0 8,0 5,0 8,5 6,5 pH pH 7,0 7,0 7,0 7,0 7,0 7,0 7,0 7,0 7,0 7,0 7,5 7,5 7,5 α-glycosidic bonds. Due to Temperature (ºC) Temperature (ºC) Optimum Optimum 20-30 90 40 40 40 40 50 20 30 20 44 45 55 55 55 42 25 75 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 vial vial 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Arabinanases hydrolyze the1,5-α ArabinaNA position inanacceptor, whichmay beglucose ora1,4-α Amylomaltases, also termed 4-α Amylomaltases Amylases ( Catalogue Catalogue Catalogue Number Number Number CZ0806 CZ0904 CZ0690 CZ0968 CZ0844 CZ0902 CZ0220 CZ0254 CZ0799 CZ0393 CZ0552 CZ0758 CZ0759 CZ1004 CZ0961 CZ0419 CZ0561 CZ0219 CZ0931 CZ1027 CZ0191 Amylomaltase 57A Amylomaltase 77A Amylomaltase 77A Amylomaltase 77A Amylomaltase 13A Amylomaltase 13A Amylomaltase 13B Glucoamylase 15A Arabinanase 43D Arabinanase 43A Arabinanase 43A Arabinanase 43A Arabinanase 43A Arabinanase 43B Arabinanase 43B Arabinanase 51A 13A β-Amylase 14B Amylase 13A Amylase 13A Amylase 13B ses cont Enzyme Enzyme Enzyme .) -linked L-arabinofuranoside backbone ofplant cell wall arabinans. -glucanotransferases, are enzymes thattransfer asegment ofa1,4- α Ruminococcus champanellensis Ruminococcus champanellensis Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Thermoactinomyces vulgaris Saccharophagus degradans Thermotoga neapolitana Paenibacillus polymyxa Thermotoga petrophila Thermus thermophilus Thermotoga maritima Thermotoga maritima Thermotoga maritima Thermotoga maritima Pyrococcus furiosus Source Organism Source Organism Source Organism Bacillus subtilis Bacillus subtilis Escherichia coli Escherichia coli Bacillus cereus -D-glucan. GH43-CBM61-doc Architecture Architecture Architecture CBM48-GH13 GH43-CBM13 CBM22-GH51 CAZ CAZ CAZ GH43 GH43 GH43 GH43 GH43 GH57 GH77 GH77 GH77 GH14 GH15 GH13 GH13 GH13 GH13 GH13 GH13 y y y 3.2.1.68 3.2.1.99 3.2.1.99 3.2.1.99 3.2.1.99 3.2.1.99 3.2.1.99 3.2.1.99 3.2.1.99 2.4.1.25 2.4.1.25 2.4.1.25 2.4.1.25 2.4.1.25 2.4.1.25 2.4.1.25 3.2.1.2 3.2.1.3 3.2.1.1 3.2.1.1 3.2.1.1 EC EC EC Optimum Optimum Optimum Optimum 7,0-8,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 8,0 6,0 6,0 6,0 6,0 6,0 6,6 5,0 6,5 pH pH pH 7,0 7,0 7,0 7,0 7,0 7,0 7,0 Temperature (ºC) Temperature (ºC) Temperature (ºC) -D-glucan to anew Optimum Optimum Optimum 90-100 85-90 60 40 50 85 65 70 70 70 25 73 37 37 37 37 37 37 37 37 37 mg/ mg/ mg/ 0,25 0,25 0,25 0,25 0,25 0,25 vial vial vial 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 13 cazymes 14 NZYTech 2021 containing 1,3-and/or 1,5-linkages, arabinoxylans andarabinogalactans. Arabinofuranosidases are exo-acting enzymes thatremove α Arabinofuranosidases Catalogue Number CZ0490 CZ0209 CZ0489 CZ0560 CZ0805 CZ0024 CZ0485 CZ0903 CZ0995 CZ0826 CZ0882 CZ0492 CZ0842 CZ0554 CZ0807 CZ0292 CZ0329 CZ0943 CZ0487 CZ0198 CZ0557 CZ0707 CZ0778 CZ0201 CZ0819 CZ0841 CZ0138 CZ0136 CZ0291 CZ0951 CZ0313 CZ1024 CZ0137 CZ1023 CZ0771 β-L-Arabinofuranosidase 146B β-L-Arabinofuranosidase 142A β-L-Arabinofuranosidase 127A β-L-Arabinofuranosidase 137A β-L-Arabinofuranosidase 137A Dha hydrolase 143A &β-L- Arabinofuranosidase 142A Arabinofuranosidase 121A Arabinofuranosidase 62A Arabinofuranosidase 43A Arabinofuranosidase 43D Arabinofuranosidase 43A Arabinofuranosidase 43A Arabinofuranosidase 43A Arabinofuranosidase 43A Arabinofuranosidase 43D Arabinofuranosidase 43B Arabinofuranosidase 43B Arabinofuranosidase 43B Arabinofuranosidase 43A Arabinofuranosidase 43C Arabinofuranosidase 43B Arabinofuranosidase 43B Arabinofuranosidase 43C Arabinofuranosidase 43E Arabinofuranosidase 43E Arabinofuranosidase 43F Arabinofuranosidase 51A Arabinofuranosidase 51A Arabinofuranosidase 51A Arabinofuranosidase 51A Arabinofuranosidase 51A Arabinofuranosidase 51A Arabinofuranosidase 51A Arabinofuranosidase 51B Arabinofuranosidase 51B & β-Glucuronidase 2A 10BA & Enzyme Ruminococcus champanellensis Ruminococcus champanellensis Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bifidobacterium adolescentis Clostridium thermocellum Clostridium thermocellum Streptomyces avermitilis Clostridium stercorarium Bifidobacterium longum Bifidobacterium longum Thermotoga maritima Podospora anserina Cellvibrio japonicus Cellvibrio japonicus Cellvibrio japonicus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Source Organism Ustilago maydis Bacillus subtilis Bacillus subtilis -L-arabinosides from α CBM22-GH10- M22- CBM57-CBM35 Architecture GH143-GH142 GH43-CBM32 GH43-CBM75 GH43-CBM6 GH43-CBM6 GH137-GH2- GH43-CBM6 GH146 GH142 GH127 GH137 GH121 CAZ GH62 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH51 GH51 GH51 GH51 GH51 GH51 GH51 GH51 GH51 y 3.2.1.185 and and 3.2.1.185 3.2.1.37 and 3.2.1.8 and 3.2.1.185 3.2.1.185 3.2.1.185 3.2.1.185 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.55 3.2.1.31 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- EC -L-arabinofuranosides, Optimum Optimum 5,0-6,0 5,0-6,5 6,0-8,0 4,0-6,0 5,0-6,0 5,5-6,0 4,0-7,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,0 6,0 pH 4,5 6,5 5,5 5,5 7,0 7,0 7,0 7,0 7,0 7,0 7,0 7,2 7,2 7,2 7,2 7,2 Temperature (ºC) Optimum 80-90 35-40 37-60 37-45 50 65 65 30 50 45 70 25 25 25 25 25 35 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 α -L-arabinans mg/ 0,25 0,25 0,25 0,25 0,25 vial 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 from thereducing ornon-reducing carbohydrate chainends. by releasing two to four unitsofglucose from thepolysaccharide ends. Cellobiohydrolases may cleave progressively cellulose Cellobiohydrolases are cellulases thatdegrade cellulose by hydrolysing 1,4-β Cellobiohydrolases are found insea andgut . Carrageenases degrade carrageenans, afamily oflinear sulphated from red edible seaweeds. These enzymes C backbone ofarabinoxylans. Arabinoxylanases are xylanases thatuse anarabinose side-chainasaspecificity determinant and, thus, only cleave the Arabinoxylanases Arabinopyranosidases are enzymes thatdisplay hydrolytic activity towards α Arabinopyranosidases Catalogue Catalogue Catalogue Catalogue Number Number Number Number CZ0060 CZ0909 CZ0905 CZ0056 CZ0420 CZ0982 CZ0454 CZ0760 CZ0295 CZ0293 CZ0061 CZ0753 CZ0017 CZ0176 CZ0181 arrageenases Reducing-end cellobiohydrolase 48A Reducing-end cellobiohydrolase 48A α-L-Arabinopyranosidase 2A Cellobiohydrolase 48A Cellobiohydrolase 48A Cellobiohydrolase 48A Cellobiohydrolase 6A Cellobiohydrolase 9A Cellobiohydrolase 6B Cellobiohydrolase 6B Cellobiohydrolase 5A K-Carrageenase 16A K-Carrageenase 16A Arabinoxylanase 5A Arabinoxylanase 5A Enzyme Enzyme Enzyme Enzyme Ruminococcus champanellensis Bacteroides thetaiotaomicron Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium cellulolyticum Zobellia galactanivorans Clostridium stercorarium Clostridium thermocellum Clostridium thermocellum Zobellia galactanivorans Podospora anserina Podospora anserina Thermobifida fusca Thermobifida fusca Source Organism Source Organism Source Organism Source Organism CBM13-FN-CBM62 Architecture Architecture Architecture Architecture CBM2-GH48 GH16-CBM6 GH5-CBM6- GH5-CBM6 CBM2-GH6 CBM3-GH5 CBM1-GH6 GH48 GH48 GH48 CAZ CAZ CAZ CAZ GH48 GH16 GH9 GH6 GH2 -L-arabinopyranoside. -D-glycosidic bondsinanexo-mode ofaction, y y y y 3.2.1.176 3.2.1.176 3.2.1.83 3.2.1.83 3.2.1.91 3.2.1.91 3.2.1.91 3.2.1.91 3.2.1.91 3.2.1.91 3.2.1.91 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- EC EC EC EC Optimum Optimum Optimum Optimum Optimum 5,0-6,0 5,0-8,0 5,0-8,0 5,0-9,0 5,0-9,0 5,5-6,0 5,5-6,5 5,5-6,5 6,5-7,5 6,5-7,5 6,0 6,6 5,0 5,0 pH pH pH pH 7,0 Temperature (ºC) Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum Optimum 55-60 55-60 20-30 70-75 60 60 60 60 40 50 30 65 37 37 37 mg/ mg/ mg/ mg/ 0,25 0,25 vial vial vial vial 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 15 cazymes 16 NZYTech 2021 linkages incellulose butalso inthehemicelluloses, β lose, one of the most abundant carbon and energy sources on Earth. These enzymes perform the hydrolysis of the 1,4-β Cellulases are highly effective enzymes produced by fungi, bacteria, protozoa andanimals (insects) for thedecomposition of cellu- Cellulases units. Cellodextrinases are enzymes thatparticipate inthehydrolysis of1,4-linkages in1,4-β Cellodextrinases Catalogue Catalogue Number Number CZ0008 CZ0009 CZ0062 CZ0053 CZ0023 CZ0296 CZ0236 CZ0768 CZ0294 CZ0203 CZ0010 CZ0235 CZ0016 CZ0180 CZ0014 CZ0164 CZ0013 CZ0102 CZ0272 CZ0218 CZ0183 CZ0174 CZ0011 CZ0173 CZ0217 CZ0115 CZ0112 Cellulase 5C&Acetyl xylan esterase Lichenase 26A&Cellulase 5E Lichenase 26A&Cellulase 5E Cellulase 124A Cellulase 48A Cellulase 44A Cellulase 131A Cellulase 16A Cellulase 12A Cellulase 12B Cellodextrinase 5A Cellulase 8A Cellulase 8A Cellulase 9A Cellulase 5A Cellulase 6A Cellulase 5A Cellulase 9B Cellulase 5A Cellulase 5D Cellulase 6B Cellulase 5B Cellulase 5A Cellulase 5B Cellulase 5E Cellulase 5E Cellodextrinase 1A Enzyme 2A Enzyme Cellvibrio mixtus xClostridium Ruminococcus flavefaciens Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Thermotoga maritima Thermotoga maritima Podospora anserina Podospora anserina Thermobifida fusca Cellvibrio japonicus Source Organism Cellvibrio mixtus Cellvibrio mixtus Bacillus subtilis Bacillus subtilis Escherichia coli thermocellum Ruminococcus flavefaciens Clostridium cellulolyticum -glucan andxyloglucan. Enzymes inthiscategory refer to endo-actingcellulases. Source Organism Architecture GH131-CBM1 GH26-GH5- GH5-CBM11 GH26-GH5 GH5-CE2 GH124 CBM11 GH48 GH44 CAZ GH16 GH12 GH12 GH8 GH8 GH9 GH9 GH6 GH5 GH5 GH5 GH6 GH5 GH5 GH5 GH5 GH5 Architecture y CAZ GH5 GH1 y 3.2.1.73 and 3.2.1.73 and 3.2.1.4 and 3.1.1.72 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.- EC 3.2.1.74 3.2.1.74 EC -D-glucans, to remove successive Optimum Optimum Optimum Optimum 4,5-8,0 4,0-6,0 4,0-6,0 6,0-8,0 6,5-8,0 6,0-8,5 5,0-6,0 6,0-6,5 4,5-8,0 5,0-9,0 5,0-7,5 4,5-7,0 7,0-8,0 7,0-8,0 6,0-7,0 5,0-7,0 5,0-7,0 6,0 6,0 6,0 6,0 6,5 pH pH 6,2 7,0 7,0 7,0 7,5 Temperature (ºC) Temperature (ºC) Optimum Optimum 60 60 60 60 60 60 60 40 60 80 40 90 50 50 40 50 40 65 50 65 65 65 85 79 37 37 37 -D-glycosidic mg/ mg/ 0,25 0,25 0,25 vial vial 0,6 0,5 3,5 2,2 2 2 2 2 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Cellulases (CONT.) Catalogue Number CZ0409 CZ0404 CZ0906 CZ0908 CZ0306 CZ0390 CZ0446 CZ0864 CZ0896 CZ0403 CZ0899 CZ0865 CZ0564 CZ0895 CZ0920 CZ0434 CZ0843 CZ0392 CZ0334 CZ0907 CZ0679 CZ0897 CZ0479 CZ0476 CZ0447 CZ0461 CZ0477 CZ0381 CZ0391 CZ0914 CZ0451 CZ0413 CZ0412 CZ0913 CZ0921 Cellulase 5B&Mannanse 5A Cellulase 44A Cellulase 48A Cellulase 44A Cellulase 44A Cellulase 9W Cellulase 12A Cellulase 12A Cellulase 9M Cellulase 9Q Cellulase 9A Cellulase 9R Cellulase 5A Cellulase 8A Cellulase 9A Cellulase 5D Cellulase 6A Cellulase 5B Cellulase 9T Cellulase 5A Cellulase 8C Cellulase 9R Cellulase 5B Cellulase 5B Cellulase 5A Cellulase 5B Cellulase 5D Cellulase 5B Cellulase 5C Cellulase 5G Cellulase 5B Cellulase 9E Cellulase 5C Cellulase 5C Cellulase 9J Enzyme Caldicellulosiruptor saccharolyticus Ruminiclostridium thermocellum Ruminiclostridium thermocellum Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Clostridium cellulolyticum Clostridium cellulolyticum Clostridium cellulolyticum Clostridium cellulolyticum Clostridium cellulolyticum Clostridium cellulolyticum Clostridium thermocellum Clostridium cellulolyticum Clostridium cellulolyticum Clostridium cellulolyticum Caldicellulosiruptor bescii Clostridium cellulovorans Thermotoga neapolitana Clostridium cellulovorans Clostridium cellulovorans Bacillus licheniformis Bacillus licheniformis Ruminococcus albus Bacillus halodurans Thermobifida fusca Thermobifida fusca Thermobifida fusca Source Organism Bacillus sp, GH44-CBM44 GH44-CBM44 Architecture GH5-doc-doc GH5-CBM46 GH9-CBM3 GH6-CBM2 GH9-CBM3 GH5-CBM3 CBM2-GH5 GH5-GH5 GH5-Doc GH5-Doc GH44 GH48 CAZ GH12 GH12 GH8 GH9 GH9 GH9 GH9 GH9 GH9 GH9 GH5 GH5 GH5 GH8 GH9 GH5 GH5 GH5 GH5 GH5 GH5 y 3.2.1.4 and 3.2.1.151 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.- 3.2.1.- EC Optimum Optimum 4,0-6,0 4,0-6,0 5,0-6,0 7,0-8,0 8,6-9,0 7,0-8,0 6,0-7,0 6,5-7,5 6,0 6,0 6,0 8,0 6,0 6,0 6,0 6,0 6,0 6,0 6,0 6,8 6,0 6,5 5,0 6,5 5,0 5,0 5,0 5,0 6,8 6,8 pH 5,5 5,2 7,0 7,0 7,0 Temperature (ºC) Optimum 55-60 78,5 80 60 40 40 60 48 60 50 95 65 65 55 45 70 70 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ 0,25 0,25 vial 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

17 cazymes 18 NZYTech 2021 walls offungiandtheexoskeletal elements ofsome animals (including worms andarthropods). Chitinases are hydrolytic enzymes thatbreak down the1,4-β Chitinases Cellulases (CONT.) Catalogue Catalogue Number Number CZ0030 CZ0948 CZ0986 CZ0946 CZ0984 CZ0999 CZ0994 CZ0985 CZ0298 CZ0926 CZ0438 CZ0993 CZ0924 CZ0925 CZ0923 CZ0953 CZ0922 CZ0967 CZ0947 CZ0748 CZ0763 CZ0742 CZ1006 CZ0991 CZ1042 CZ0731 CZ1001 Cellulase 124A Chitinase 18A Chitinase 18A Chitinase 18A Chitinase 18A Chitinase 19A Chitinase 18A Chitinase 19A Cellulase 74A Cellulase 8A Cellulase 9D Cellulase 9A Cellulase 9B Cellulase 9G Cellulase 9G Cellulase 9B Cellulase 9B Cellulase 9K Cellulase 5A Cellulase 5A Cellulase 9C Cellulase 5A Cellulase 9E Cellulase 9C Cellulase 9C Cellulase 9F Cellulase 9F Enzyme Enzyme Ruminiclostridium thermocellum Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Streptomyces thermoviolaceus Bacteroides thetaiotaomicron Saccharophagus degradans Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Clostridium papyrosolvens Clostridium papyrosolvens Clostridium thermocellum Clostridium cellulolyticum Caldicellulosiruptor bescii Streptomyces coelicolor Clostridium clariflavum Clostridium clariflavum Thermotoga maritima Bacillus licheniformis Thermobifida fusca Source Organism Source Organism Bacillus cereus -D-glucosaminide linkages inchitin, acomponent ofthecell GH9-CBM3-doc- GH9-CBM3-doc- GH9-CBM3-Doc GH9-CBM3-Doc GH9-CBM3-Doc GH9-CBM3-Doc GH5-CBM28-S- CBM4-CBM30- CBM4-CBM30- CBM4-CBM30- Architecture Architecture GH9-CBM3- CBM4-GH9 GH9-CBM2 GH9-CBM3 GH9-CBM3 GH124-Doc CBM3-doc GH8-Doc GH9-Doc GH9-doc LH-SLH CAZ CAZ GH74 GH18 GH18 GH18 GH18 GH19 GH18 GH19 GH9 GH5 GH5 doc doc y y 3.2.1.14 and 3.2.1.14 3.2.1.14 3.2.1.14 3.2.1.14 3.2.1.14 3.2.1.14 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.4 3.2.1.- EC EC Optimum Optimum Optimum 5,0-6,0 5,0-6,0 5,0-6,0 5,5-6,0 5,0-7,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,0 6,0 4,0 6,0 6,8 6,8 6,8 6,8 6,0 5,0 6,5 pH pH 5,5 5,5 7,5 7,5 Temperature (ºC) Temperature (ºC) Optimum Optimum 55-60 45-55 60 60 60 90 60 65 65 25 75 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 vial vial 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 dues inβ β Fructofuranosidases Fructanases are enzymes thatparticipate inthehydrolysis offructans, including inulin andlevan. Fructanases units from thenon-reducing endsofthechains. are enzymes thatparticipate inthehydrolysis of1,6-α Dextranases participate intheendo-hydrolysis of1,6-α Dextranases and N-acetyl-D-glucosamine (acetylated unit). tosan. Chitosan isalinear polysaccharide composed ofrandomly distributed β Chitosanases participate intheendo-hydrolysis of1,4- β Chitosanases Catalogue Catalogue Catalogue Catalogue Number Number Number Number -Fructofuranosidases are enzymes thatparticipate inthehydrolysis ofterminal non-reducing β CZ0965 CZ0569 CZ0426 CZ0692 CZ0780 CZ0526 CZ0978 CZ0676 CZ0720 CZ0547 CZ0437 CZ0745 CZ0125 CZ0781 -D-fructofuranosides. Dextran 1,6-α Fructan 2,1-β-fructosidase 32A β-Fructofuranosidase 100B β-Fructofuranosidase 68A β-Fructofuranosidase 32A β-Fructofuranosidase 32B Isomalto- 27A Chitosanase 46A Dextranase 66A Dextranase 49A Fructanase 32A Fructanase 32A Chitosanase 8B Chitosanase 5A -isomaltotriosidase 49A Enzyme Enzyme Enzyme Enzyme Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Arthrobacter dextranolyticus Paenibacillus polymyxa Lactobacillus paracasei Brevibacterium fuscum Streptococcus mutans Streptococcus mutans Arthrobacter oxydans Streptomyces griseus Source Organism Source Organism Source Organism Source Organism Arthrobacter sp. Bacillus subtilis Bacillus cereus Nostoc sp. -D-glucosidic linkages indextrans, to remove successive isomaltotriose -D-glucosidic linkages indextran. Dextran 1,6-α -linkages between D-glucosamine residues inpartly acetylated chi- CBM66-GH32 Architecture Architecture Architecture Architecture GH27-CBM35 GH100 GH68 GH66 GH46 GH49 GH49 CAZ CAZ CAZ CAZ GH32 GH32 GH32 GH32 GH8 GH5 y y y y -1-4-linked D-glucosamine (deacetylated unit) 3.2.1.64 and 3.2.1.64 and 3.2.1.153 3.2.1.132 3.2.1.132 3.2.1.132 3.2.1.26, 3.2.1.26, 3.2.1.80 3.2.1.80 3.2.1.80 3.2.1.80 3.2.1.94 3.2.1.26 3.2.1.26 3.2.1.95 3.2.1.11 3.2.1.11 EC EC EC EC Optimum Optimum Optimum Optimum Optimum Optimum 8,0-9,0 5.0-5.5 4,0-7.0 7,5-7,8 8,0 8,0 6,0 5,5 pH pH pH pH 5,5 3,5 7,0 7,0 5,7 7,5 -D-fructofuranoside resi- Temperature (ºC) Temperature (ºC) Temperature (ºC) Temperature (ºC) -isomaltotriosidases Optimum Optimum Optimum Optimum 60 60 60 40 50 49 30 35 35 37 37 37 37 37 mg/ mg/ mg/ mg/ 0,25 0,25 vial vial vial vial 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 19 cazymes 20 NZYTech 2021 Galactanases are enzymes thatspecifically hydrolyze β G Fucosidases cleave fucosidic bondsinoligosaccharides andglycoconjugates. Fucosidases Catalogue Catalogue Number Number CZ0804 CZ0989 CZ0054 CZ0028 CZ0029 CZ0566 CZ0299 CZ0963 CZ0754 CZ0792 CZ1088 CZ1056 CZ1058 CZ1054 CZ1059 CZ1055 CZ0761 CZ0178 CZ1087 CZ0127 CZ1057 CZ0511 alactanases Exo-1,3-β-Galactanase 43A Methyl-fucosidase 139A β-D-Fucosidase 30A Galactanase 53A Galactanase 53A Galactanase 53A Galactanase 53A Galactanase 53A Fucosidase 141A Fucosidase 95A Fucosidase 95A Fucosidase 95A Fucosidase 95D Fucosidase 29A Fucosidase 29A Fucosidase 29A Fucosidase 95B Fucosidase 29A Fucosidase 29B Fucosidase 95C Fucosidase 29E Fucosidase 29F Enzyme Enzyme Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Clostridium thermocellum Clostridium thermocellum Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Bifidobacterium longum Clostridium perfringens Thermotoga maritima Bacillus licheniformis Cellvibrio japonicus Source Organism Source Organism Lactobacillus casei Prevotella bryantii Bacillus subtilis -D-galactosidic linkages ingalactans andarabinogalactans. Architecture Architecture GH29-CBM32 GH139 GH141 GH95 GH30 GH95 CAZ CAZ GH95 GH95 GH95 GH29 GH95 GH29 GH29 GH43 GH29 GH29 GH29 GH53 GH53 GH53 GH53 GH53 y y 3.2.1.145 3.2.1.89 3.2.1.89 3.2.1.89 3.2.1.89 3.2.1.89 3.2.1.38 3.2.1.63 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.51 3.2.1.- EC EC Optimum Optimum Optimum 3,0-10,0 6,0-10,0 6,5-8,0 4,5-5,0 5,0-7,5 6,0-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 5,0 6,5 pH pH 7,0 7,0 Temperature (ºC) Temperature (ºC) Optimum Optimum 60 60 50 65 42 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 0,25 0,25 0,25 vial vial 0,5 0,5 0,5 0,5 1,5 2 1 1 1 1 1 1 1 1 1 1 1 lactosides, including galactose oligosaccharides, galactomannans andgalactolipids. ipate intheendohydrolysis of1,4-β N-acetylgalactosaminyl residues anda1,2-α Blood-group endo-β β β α α Catalogue Catalogue -Galactosidases are enzymes thatspecifically hydrolyze terminal non-reducing β Number Number -Galactosidases are enzymes thatparticipate inthehydrolysis ofterminal, non-reducing α CZ0686 CZ0200 CZ0684 CZ0026 CZ0384 CZ0399 CZ0493 CZ0238 CZ0555 CZ0705 CZ0225 CZ0223 CZ0322 CZ0782 CZ0199 CZ0196 CZ1050 CZ1064 CZ0414 CZ0195 CZ0516 CZ0155 CZ0710 CZ0153 CZ0701 CZ0411 -G - G alactosidases alactosidases N-Acetylglucosamine endo-β- α-Galactosidase 110A α-Galactosidase 110A Blood-group endo-β- α-Galactosidase 110A α-Galactosidase 110A α-Galactosidase 110B β-Galactosidase 50A α-Galactosidase 95A α-Galactosidase 95A β-Galactosidase 42A β-Galactosidase 42A β-Galactosidase 42A α-Galactosidase 36A α-Galactosidase 36A β-Galactosidase 42A β-Galactosidase 42B α-Galactosidase 97A α-Galactosidase 97B α-Galactosidase 27A α-Galactosidase 27A α-Galactosidase 4A α-Galactosidase 4A β-Galactosidase 2A β-Galactosidase 2B β-Galactosidase 1A galactosidase 98A galactosidase 16C -galactosidases are enzymes thathydrolyze the1,4-β Enzyme Enzyme -D-galactosidic linkages inkeratan sulfate. Caldicellulosiruptor saccharolyticus Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Streptococcus pneumoniae -D-fucosyl residue. Keratan-sulfate endo-1,4-β Clostridium cellulolyticum Zobellia galactanivorans Bifidobacterium bifidum Bifidobacterium longum Bifidobacterium longum Clostridium perfringens Sulfolobus solfataricus Thermotoga maritima Thermotoga maritima Thermotoga maritima Bacillus halodurans Bacteroides ovatus Bacteroides fragilis Bacteroides fragilis Victivallis vadensis Source Organism Source Organism Cellvibrio mixtus Bacillus subtilis Escherichia coli Architecture Architecture -D-galactosyl linkages adjacent to a1,3-α GH110 GH110 GH110 GH110 GH110 GH98 GH50 GH95 GH95 CAZ CAZ GH42 GH42 GH42 GH36 GH36 GH42 GH42 GH97 GH97 GH27 GH27 GH16 GH4 GH4 GH2 GH2 GH1 y y -D-galactose residues inβ 3.2.1.102 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.22 3.2.1.23 3.2.1.23 3.2.1.22 3.2.1.23 3.2.1.22 3.2.1.22 3.2.1.22 3.2.1.22 3.2.1.22 3.2.1.22 3.2.1.22 3.2.1.22 3.2.1.22 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- EC EC -galactosidases are enzymes thatpartic -D-galactose residues inα Optimum Optimum Optimum 4,0-12,0 8,0-9,5 6,0-6,5 4,5-7,0 6,5-7,5 6,5-7,5 6,5-7,5 7,0-7,5 6,0 8,0 6,8 5,0 6,5 6,5 6,5 6,5 6,5 5,5 pH pH 7,0 7,0 7,0 7,0 7,0 7,4 7,4 7,2 Temperature (ºC) Temperature (ºC) Optimum Optimum 28-30 30-37 -D-galactosides. -D-galactosyl or 80 80 80 40 50 30 30 45 26 25 25 37 37 37 37 37 37 37 37 37 37 37 37 37 -D-ga- mg/ mg/ 0,25 0,25 0,25 0,25 vial vial 0,5 0,5 0,5 0,5 0,5 1,2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 - 21 cazymes 22 NZYTech 2021 These enzymes are particular β β galactose-6-phosphate andotherorganic molecules. Phospho-β P β GalRha isfound inrhamnogalacturonan I(RG-I) where onegalactose residue bindsL-Rha inthemainchainofRG-I. Catalogue Catalogue Catalogue Number Number Number CZ0808 CZ0966 CZ0850 CZ0854 CZ0970 CZ0542 CZ0788 CZ0730 CZ0974 CZ0323 CZ0333 CZ0927 CZ0783 CZ0774 CZ0851 CZ0261 CZ0314 -D-G hospho-β - G alactosidases (CONT.) alactosyl -galactosidases are enzymes thathydrolyze theβ β-D-Galactosyl-1,4-L-rhamnose β-D-Galactosyl-1,4-L-rhamnose Endo-1,4-β-galactosidase 98A Phospho-β-Galactosidase 1A Phospho-β-Galactosidase 1A Phospho-β-Galactosidase 1B Phospho-β-Galactosidase 1B β-Galactosidase 42A β-Galactosidase 42A β-Galactosidase 35A β-Galactosidase 35A β-Galactosidase 35A β-Galactosidase 2A β-Galactosidase 2D β-Galactosidase 2A β-Galactosidase 2A phosphorylase 112A phosphorylase 112A β-Galactosidase 2C Enzyme Enzyme Enzyme -G alactosidases -1,4-L-rhamnose phosphorylases -galactoside phosphorylases actingspecifically onD-galactosyl-1,4-β Alicyclobacillus acidocaldarius Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Paenibacillus thiaminolyticus Streptococcus pneumoniae Streptococcus pneumoniae Bifidobacterium longum Streptococcus mutans Lactobacillus gasseri Lactobacillus gasseri Bacteroides ovatus Bacteroides fragilis Source Organism Source Organism Source Organism Lachnoclostridium Lactococcus lactis phytofermentans Opitutus terrae -glycosidic bondbetween aterminal nonreducing Architecture Architecture Architecture GH43-GH42 GH98 GH112 GH112 CAZ CAZ CAZ GH42 GH35 GH35 GH35 GH2 GH2 GH2 GH2 GH2 GH1 GH1 GH1 GH1 y y y 3.2.1.23 and 2.4.1.247 2.4.1.247 3.2.1.102 3.2.1.85 3.2.1.85 3.2.1.85 3.2.1.85 3.2.1.38 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 3.2.1.23 EC EC EC Optimum Optimum Optimum Optimum Optimum 5,5-6,0 5,5-8,0 7,0-8,0 5,5-6,5 6,0-7,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,0-70 8,0 4,0 5,0 6,5 pH pH pH 5,5 7,4 7,4 -L-rhamnose (GalRha). Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 45-55 40 50 65 65 45 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ mg/ vial vial vial 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 sidic bonds. NZYTech makes available arange ofdextransucrases (transfer to the1, tracellular enzymes showing strictsubstrate specificity. Phosphorylases, whichreversibly phosphorolyze oligosaccharides to produce monosaccharide 1-phosphate are generally in- Glucansucrases are enzymes thatconvert sucrose into α Glucansucrases Galacturonidases are enzymes thatattack homoandrhamnogalacturonans. G These enzymes are β G Catalogue Catalogue Catalogue Number Number Number CZ0849 CZ0928 CZ0266 CZ0546 CZ0265 CZ0852 CZ0545 CZ0853 CZ0847 CZ0267 CZ0767 CZ1005 CZ0775 CZ1002 alacturonidases alactosyl 1,3-β-Galactosyl-N-acetylhexosa- 1,3-β-Galactosyl-N-acetylhexosa- Exo- Exo- Exo- Exo- Exo- Exo- mine phosphorylase 112A mine phosphorylase 112A β-D-Galacturonidase 2A α-galacturonidase 138A Glucansucrase 70D Glucansucrase 70A Glucansucrase 70A Glucansucrase 70B Glucansucrase 70C α-galacturonidase 28A α-galacturonidase 28D α-galacturonidase 28B α-galacturonidase 28C α-galacturonidase 28E Enzyme Enzyme Enzyme -galactoside phosphorylases specifically attacking β -N- acetylhexosamine phosphorylases Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Clostridium phytofermentans Bifidobacterium longum Streptococcus mutans Streptococcus mutans Streptococcus mutans Lactobacillus animalis Lactobacillus reuteri Source Organism Source Organism Source Organism -glucan polysaccharides with1 Architecture Architecture Architecture GH138 GH112 GH112 GH28 CAZ GH28 CAZ CAZ GH28 GH28 GH28 GH70 GH70 GH70 GH70 GH70 GH2 y y y -D-galactopyranosyl-1,3-N-acetyl-D-glucosamine. 2.4.1.211 2.4.1.211

6-α 2.4.4.- 2.4.1.5 2.4.1.5 2.4.1.5 2.4.1.5 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- EC EC EC ,2-α bond). , 1 , ,3- Optimum Optimum Optimum Optimum 6,0-7,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 α 6,0 6,0 6,0 8,5 5,8 pH pH pH 5,5 , 1 , ,4- α Temperature (ºC) Temperature (ºC) Temperature (ºC) and/or 1, Optimum Optimum Optimum 22-59 40 37 37 37 37 37 37 37 37 37 37 37 37

6-α -glyco mg/ mg/ mg/ 0,25 vial vial vial 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 - 23 cazymes 24 NZYTech 2021 1,6-α ticipate inhydrolysis of1,6-α cosidases participate in endohydrolysis of 1,3-α D-glucosamine residues from thenon-reducing termini ofthecarbohydrate chain. β β α α Exo- Glucosaminidases Catalogue Catalogue -Glucosidases are enzymes that participate inthehydrolysis ofterminal, non-reducing β -Glucosidases are enzymes thatcleave theglycosidic bondbetween two glucose molecules, occurring asα Number Number CZ0268 CZ0958 CZ0264 CZ0962 CZ0952 CZ0328 CZ0524 CZ0252 CZ0523 CZ0674 CZ0567 CZ0019 CZ0387 CZ0301 CZ0318 CZ0812 CZ0551 CZ0101 CZ0811 CZ0211 CZ0111 CZ1011 -Glucosidases -Glucosidases SKU β -D-glucosidic linkages insome oligosaccharides produced from starch andglycogen by α -Glucosaminidases are enzymes thatparticipate inthehydrolysis ofchitosan orchitosan oligosaccharides to remove successive Exo- Exo- Oligo-1,6-α-Glucosidase 13A Oligo-1,6-α-Glucosidase 13A Oligo-1,6-α-Glucosidase 13B Phospho-α-Glucosidase 4A Phospho-α-Glucosidase 4A Exo- 1,3-α-Glucosidase 87A β-Glucosaminidase 35A β-Glucosaminidase 35A α-Glucosidase 63A α-Glucosidase 97A β-Glucosaminidase 9A α-Glucosidase 13A α-Glucosidase 31A α-Glucosidase 31A α-Glucosidase 13A α-Glucosidase 31B β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1B Enzyme Enzyme Enzyme -D-glucosidic linkages in1,6-α Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bifidobacterium adolescentis Photobacterium profundum Thermococcus kodakarensis Saccharophagus degradans Clostridium acetobutylicum Clostridium thermocellum Paenibacillus polymyxa Sulfolobus solfataricus Thermus thermophilus Streptococcus mutans Thermotoga maritima Pyrococcus horikoshii Tannerella forsythia -D-glucosidic linkages in isolichenin, pseudonigeran and nigeran. These enzymes par Thermobifida fusca Source Organism Source Organism Source Organism Bacillus circulans Bacillus subtilis Bacillus subtilis Escherichia coli Escherichia coli Bacillus cereus -D-glucans andderived oligosaccharides. They participate inhydrolysis of Architecture Architecture Architecture CBM34-GH13 CAZ CAZ CAZ GH35 GH63 GH35 GH97 GH87 GH13 GH13 GH13 GH13 GH31 GH31 GH31 GH9 GH4 GH4 GH1 GH1 GH1 GH1 GH1 GH1 y y y -D-glucosyl residues withrelease ofβ 3.2.1.20 and 3.2.1.165 3.2.1.165 3.2.1.165 3.2.1.122 3.2.1.122 3.2.1.20 3.2.1.84 3.2.1.20 3.2.1.20 3.2.1.20 3.2.1.20 3.2.1.59 3.2.1.70 3.2.1.10 3.2.1.10 3.2.1.10 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 EC EC EC -amylases andisomaltose. Optimum Optimum Optimum Optimum Optimum 5,0-6,0 5,0-8,0 5,5-9,0 5,5-7,0 6,0 6,0 6,5 6,5 5,0 6,5 6,5 6,5 6,5 pH pH pH 7,0 7,0 7,0 7,0 7,0 7,0 7,5 7,5 7,5 - configurations. 1,3-α Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 45-50 45-50 80 60 90 50 50 30 85 25 35 37 37 37 37 37 37 37 37 37 37 37 -D-glucose. -Glu- mg/ mg/ mg/ 0,25 0,25 0,25 0,25 0,25 0,25 0,25 vial vial vial 0,5 0,5 0,5 0,5 0,5 0,5 3 1 1 1 1 1 1 1 1 - Phospho-β-glucosidases catalyze thehydrolysis of1,4-β-linked cellobiose 6-phosphate to yield glucose andglucose 6-phosphate. Phospho-β β Catalogue Catalogue Number Number CZ0098 CZ0099 CZ0980 CZ0894 CZ0449 CZ0444 CZ0260 CZ0396 CZ0097 CZ0455 CZ0259 CZ0786 CZ0262 CZ0525 CZ0100 CZ0397 CZ0457 CZ0973 CZ0160 CZ0818 CZ0418 CZ0510 CZ0919 CZ0441 CZ0772 CZ0319 CZ0163 CZ0777 CZ0212 CZ0161 -Glucosidases ( Phospho-β-Glucosidase 4A Phospho-β-Glucosidase 4A Phospho-β-Glucosidase 4A Phospho-β-Glucosidase 1A Phospho-β-Glucosidase 1A Phospho-β-Glucosidase 1A Phospho-β-Glucosidase 1D Phospho-β-Glucosidase 1B Phospho-β-Glucosidase 1B Phospho-β-Glucosidase 1B Phospho-β-Glucosidase 1C β-Glucosidase 3A β-Glucosidase 3A β-Glucosidase 3A β-Glucosidase 3A β-Glucosidase 3A β-Glucosidase 3B β-Glucosidase 3B β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1A β-Glucosidase 1G β-Glucosidase 1C Enzyme Enzyme -Glucosidases cont Caldicellulosiruptor saccharolyticus Alicyclobacillus acidocaldarius bacterium enrichmentculture Pectobacterium carotovorum Pectobacterium carotovorum Pectobacterium carotovorum Saccharophagus degradans Clostridium cellulolyticum Caldicellulosiruptor bescii Clostridium cellulovorans Paenibacillus polymyxa .) Thermotoga petrophila Streptococcus mutans Streptococcus mutans Streptococcus mutans Thermotoga maritima Ruminococcus albus Bacillus halodurans Pyrococcus furiosus Cellvibrio japonicus Bacteroides ovatus Bacteroides fragilis Source Organism Source Organism Prevotella bryantii Bacillus subtilis Bacillus subtilis Bacillus subtilis Bacillus subtilis Escherichia coli Escherichia coli Escherichia coli clone Architecture Architecture CAZ CAZ GH4 GH4 GH4 GH3 GH3 GH3 GH3 GH3 GH3 GH3 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 y y 3.2.1.86 and 3.2.1.21 and 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.74 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 3.2.1.21 EC EC Optimum Optimum Optimum 6,5-10,0 6,0-6,5 6,0-6,5 5,0-7,0 7,0-8,0 7,0-8,0 6,5-7,5 6,5-7,5 7,0-7,9 8,0 6,0 6,0 8,0 6,0 8,0 6,0 8,0 5,0 6,8 5,0 6,8 pH pH 5,5 7,0 7,0 7,0 7,0 7,0 7,5 7,5 7,5 Temperature (ºC) Temperature (ºC) Optimum Optimum 100-110 25-50 90 40 40 40 40 50 30 30 85 65 45 39 70 25 35 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 0,25 0,25 0,25 vial vial 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 25 cazymes 26 NZYTech 2021 determinant for theglucurono sidechain. Glucuronoxylanases are enzymes that participate in endohydrolysis of the backbone of glucuronoxylans and thus display a specificity Glucuronidases are enzymes thatactonα Glucuronidases Phospho-β Glucuronoxylanases Catalogue Catalogue Catalogue Number Number Number CZ0020 CZ0868 CZ0869 CZ0494 CZ0855 CZ0943 CZ0445 CZ0933 CZ0709 CZ0330 CZ0795 CZ0232 CZ0729 CZ0972 CZ0916 CZ0416 CZ0277 CZ0213 CZ0316 CZ0714 CZ0315 CZ0917 CZ0311 β-L-Arabinofuranosidase 137A & α-Methyl-glucuronidase 115A Phospho-β-Glucosidase 1A Phospho-β-Glucosidase 1A Phospho-β-Glucosidase 1B Phospho-β-Glucosidase 1B Phospho-β-Glucosidase 1C Glucuronoxylanase 30A Glucuronoxylanase 30A Glucuronoxylanase 30A Glucuronoxylanase 30B α-Glucuronidase 115A β-Glucuronidase 79A α-Glucuronidase 67A α-Glucuronidase 67A α-Glucuronidase 4A α-Glucuronidase 4B β-Glucuronidase 2A β-Glucuronidase 2A β-Glucuronidase 2A β-Glucuronidase 2A β-Glucuronidase 2A β-Glucuronidase 2A Glucuronidase 67A Enzyme Enzyme Enzyme -Glucosidases (CONT.) - orβ Geobacillus stearothermophilus Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Acidobacterium capsulatum Streptococcus pneumoniae Sulfolobus solfataricus P2 Clostridium thermocellum -D-glucuronosides to release D-glucuronic acidresidues. Streptococcus pyogenes Clostridium clariflavum Clostridium clariflavum Thermotoga maritima Thermotoga maritima Ruminococcus gnavus Erwinia chrysanthemiErwinia Lactobacillus gasseri Lactobacillus gasseri Lactobacillus brevis Cellvibrio japonicus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Source Organism Source Organism Source Organism Escherichia coli CBM57-CBM35 Architecture Architecture Architecture GH30-CBM6 GH137-GH2- GH115 GH115 CAZ GH30 GH30 GH30 CAZ CAZ GH79 GH67 GH67 GH67 GH4 GH4 GH2 GH2 GH2 GH2 GH2 GH1 GH1 GH1 GH1 GH1 y y y 3.2.1.185 and 3.2.1.139 3.2.1.139 3.2.1.139 3.2.1.136 3.2.1.136 3.2.1.136 3.2.1.136 3.2.1.131 3.2.1.131 3.2.1.131 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.86 3.2.1.31 3.2.1.31 3.2.1.31 3.2.1.31 3.2.1.31 3.2.1.31 3.2.1.31 3.2.1.- EC EC EC Optimum Optimum Optimum Optimum Optimum 5,0-11,0 5,0-8,0 4,5-8,0 2,6-3,5 6,0-7,0 6,0-7,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 5,0 6,0 6,0 6,5 8,8 pH pH pH 7,0 7,0 7,5 7,0 7,2 7,2 7,5 7,2 Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 60 80 80 40 40 40 45 50 25 25 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ mg/ 0,25 0,25 0,25 0,25 vial vial vial 0,5 0,5 0,5 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Inulin fructotransferases are enzymes thatproduce α Inulases residues inhyaluronate. Hyaluronidases are enzymes thatparticipate inrandom hydrolysis of1,4-linkages between N-acetyl- β Hyaluronidases Hexosaminidases are enzymes thatare involved inthehydrolysis ofterminal N-acetyl-D-hexoxamine residues inN-acetyl-β-D-hexosaminides. Hexosaminidases eliminating thediminishing2-1-β Catalogue Catalogue Catalogue Number Number Number CZ0689 CZ0450 CZ0838 CZ0839 CZ0302 CZ0708 CZ0825 CZ0678 CZ0823 CZ0822 CZ0279 CZ0278 CZ0793 CZ0827 CZ0677 CZ0977 CZ0732 CZ0717 Inulin fructotransferase 91A Inulin fructotransferase 91A 84A Hexosaminidase 20A Hexosaminidase 20A Hexosaminidase 20A Hexosaminidase 20A Hexosaminidase 20B Hexosaminidase 20A Hexosaminidase 3A Hexosaminidase 3A Hexosaminidase 3A Hexosaminidase 3A Hexosaminidase 3A Hexosaminidase 3A Hexosaminidase 3C 84A Hyaluronidase 84B Enzyme Enzyme Enzyme -D-fructan (inulin) chainfrom theterminal D-fructosyl-D-fructosyl disaccharide. Saccharophagus degradans Saccharophagus degradans Streptococcus pneumoniae Streptococcus pneumoniae Streptococcus pneumoniae Clostridium paraputrificum Pseudomonas aeruginosa Thermotoga neapolitana Bifidobacterium longum Clostridium perfringens Clostridium perfringens Clostridium perfringens Thermotoga maritima Source Organism Source Organism Source Organism Lactobacillus casei Nonomuraea sp. Escherichia coli Vibrio furnissii Bacillus sp. -D-fructofuranose β -D-fructofuranose 1,2’:2,3’-dianhydride (DFA III)by successively GH84-CBM32- GH84-CBM32 Architecture Architecture Architecture GH20-GH20 CBM32 GH84 GH20 GH20 GH20 GH20 GH20 CAZ CAZ CAZ GH91 GH91 GH3 GH3 GH3 GH3 GH3 GH3 GH3 y y y 4.2.2.18 4.2.2.18 3.2.1.35 3.2.1.35 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 3.2.1.52 EC EC EC -D-glucosamine andD-glucuronate Optimum Optimum Optimum Optimum Optimum 5,0-8,0 5,0-6,0 5,0-5,5 7,0-8,0 7,0-8,0 6,5-7,5 5,0 5,0 5,0 5,0 5,6 6,4 6,4 pH pH pH 5,5 7,0 7,0 7,5 7,4 Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 37-40 65-75 65-75 40 50 20 65 45 37 37 37 37 37 37 37 37 37 37 mg/ mg/ mg/ 0,25 0,25 0,25 0,25 0,25 0,25 vial vial vial 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 27 cazymes 28 NZYTech 2021 abundant intheplant cell walls ofalgae, plants andfungi. ing more than3fructose units. Levanases are enzymes thatparticipate inrandom hydrolysis of2,6- β Levanases Laminarinases are generally endo-actingenzymes thatbreak down 1,3-β Laminarinases Catalogue Catalogue Number Number CZ0406 CZ0858 CZ0508 CZ0305 CZ0509 CZ0459 CZ0863 CZ0452 CZ0675 CZ0222 CZ0018 CZ1060 CZ0861 CZ0757 CZ0331 CZ1052 CZ1053 CZ0124 CZ0135 CZ0571 Laminarinase 157A Laminarinase 157A Laminarinase 64A Laminarinase 64A Laminarinase 64A Laminarinase 55A Laminarinase 81A Laminarinase 16A Laminarinase 16A Laminarinase 16A Laminarinase 16A Laminarinase 16A Laminarinase 81A Laminarinase 16A Laminarinase 16A Laminarinase 16C 32A Levanase 32A Levanase 32B Levanase 32B Enzyme Enzyme Bacteroides thetaiotaomicron Labilibaculum antarcticum Clostridium thermocellum Thermotoga neapolitana Lysobacter enzymogenes Zobellia galactanivorans Zobellia galactanivorans Zobellia galactanivorans Thermotoga petrophila Thermotoga maritima Pyrococcus furiosus Bacillus halodurans Source Organism Source Organism Paenibacillus sp. Paenibacillus sp. Paenibacillus sp. Arthrobacter sp. Polaribacter sp. Bacillus subtilis Bacillus subtilis Bacillus subtilis -D-fructofuranosidic linkages in2,6- β -glucans suchaslaminarin, callose orcurdlan. 1,3-β Architecture GH32-CBM66 Architecture GH16-CBM56 GH64-CBM6 GH16-CBM6 GH157 GH157 GH64 GH64 CAZ GH55 CAZ GH32 GH32 GH32 GH81 GH16 GH16 GH16 GH16 GH81 GH16 GH16 y y 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.39 3.2.1.65 3.2.1.65 3.2.1.65 3.2.1.65 EC EC Optimum Optimum Optimum Optimum 6,0-8,0 4,5-5,0 5,5-6,5 5,0-7,0 6,5-7,5 6,5-7,5 6,5-7,5 5,0 5,0 8,0 6,0 6,5 8,5 6,5 6,2 6,5 6,5 pH pH 7,0 7,0 7,0 -D-fructans (levans) contain- Temperature (ºC) Temperature (ºC) Optimum Optimum 70-80 85-95 78-95 60 40 40 65 45 45 30 37 37 37 37 37 37 37 37 37 41 -glucans are mg/ mg/ 0,25 0,25 vial vial 0,5 0,5 0,5 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 residues inapeptidoglycan andbetween N-acetyl-D-glucosamine residues inchitodextrins. Lysozymes are enzymes that participate in thehydrolysis of 1,4-β L which isinvolved inthelinkage to behydrolyzed isitself substituted atC-3 (EC number3.2.1.6) orC-4 (EC number3.2.1.73). Lichenases are enzymes thatparticipate intheendo-hydrolysis of1,3-1,4-β Lichenases Levansucrases are fructosyltransferases thattransfer β Levansucrases Catalogue Catalogue Catalogue Number Number Number CZ0480 CZ0898 CZ0548 yso CZ0458 CZ0053 CZ0326 CZ0423 CZ0273 CZ0015 CZ0013 CZ0012 CZ0519 CZ0719 CZ0214 CZ0128 CZ0514 CZ0713 CZ0117 zymes Lichenase 26A&Cellulase 5E Lichenase 26A&Cellulase 5E Fructosyltransferase 68A Fructosyltransferase 68A Fructosyltransferase 68A Lichenase 26A 25A Lysozyme 25A Lysozyme 23A Lysozyme 24B Lichenase 16A Lichenase 16D Lichenase 16A Lichenase 16A Lichenase 16A Lichenase 16A Lichenase 8A Lichenase 5A Enzyme Enzyme Enzyme Ruminococcus champanellensis Streptococcus pneumoniae Ruminococcus flavefaciens Ruminococcus flavefaciens Gluconacetobacter xylinus Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Paenibacillus polymyxa Clostridium perfringens Streptococcus mutans Thermotoga maritima Bacillus halodurans Source Organism Source Organism Source Organism Bacillus circulans Bacillus subtilis Bacillus subtilis Escherichia coli -D-frutosyl residue from sucrose to alevan chain. -linkages between N-acetylmuramic acidand N-acetyl-glucosamine GH16-GH16-GH16 Architecture Architecture Architecture GH26-GH5- GH26-GH5 -D-glucans whentheglucose residue, whose reducing group CBM11 GH68 GH68 CAZ GH68 GH26 CAZ GH24 GH25 GH25 GH23 CAZ GH16 GH16 GH16 GH16 GH16 GH8 GH5 y y y 3.2.1.73 and 3.2.1.73 and 3.2.1.73 3.2.1.73 3.2.1.73 3.2.1.73 3.2.1.73 3.2.1.73 3.2.1.73 3.2.1.73 2.4.1.10 2.4.1.10 2.4.1.10 3.2.1.17 3.2.1.17 3.2.1.17 3.2.1.17 3.2.1.6 3.2.1.4 3.2.1.4 EC EC EC Optimum Optimum Optimum Optimum Optimum Optimum 4,5-8,0 6,0-8,0 6,0-8,0 5,5-7,0 6,0-7,0 5,0-7,0 5,0 5,0 6,0 6,0 6,8 5,0 5,0 pH pH 4,5 pH 7,0 7,0 7,0 7,5 Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 60 60 80 60 60 30 65 30 25 32 25 37 37 37 37 37 37 37 mg/ mg/ mg/ 0,25 0,25 0,25 vial vial vial 0,6 0,5 0,5 0,5 1,5 3 2 1 1 1 1 1 1 1 1 29 cazymes 30 NZYTech 2021 and glucomannans. Plant mannanshave 1,4-β β β backbone and1,2- α and1,3-linked branches. α-Mannanases participate inthehydrolysis ofα-mannan, acell wall polysaccharide found inyeasts. This type ofmannanhasa1,6-α linked α Catalogue Catalogue -Mannanases are enzymes that participate in the random hydrolysis of 1,4-β Number Number CZ0064 CZ0550 CZ0202 CZ0464 CZ0063 CZ0308 CZ0055 CZ0288 CZ0025 CZ0289 CZ0205 CZ0992 CZ0942 CZ0442 CZ0565 CZ0425 CZ0997 CZ0479 CZ0478 CZ0270 CZ0307 CZ0146 CZ0901 CZ0681 CZ0719 CZ0215 CZ1007 CZ0123 CZ0147 -Mannanases -Mannanases Cellulase 5B&Mannanse 5A β-Mannanase 134A β-Mannanase 134A β-Mannanase 113A α-Mannanase 76A α-Mannanase 76A β-Mannanase 26A β-Mannanase 26A β-Mannanase 26A α-Mannanase 76B β-Mannanase 26B β-Mannanase 26A β-Mannanase 26B β-Mannanase 26B β-Mannanase 26E β-Mannanase 26C β-Mannanase 5A β-Mannanase 5A β-Mannanase 5A β-Mannanase 5A β-Mannanase 5A β-Mannanase 5A β-Mannanase 5A β-Mannanase 5A β-Mannanase 5A β-Mannanase 5B β-Mannanase 5B Lysozyme 24B Mannanse 5A Enzyme Enzyme Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Alicyclobacillus acidocaldarius Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Ruminococcus flavefaciens Ruminococcus flavefaciens Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium cellulolyticum Clostridium cellulovorans Clostridium cellulovorans Paenibacillus polymyxa Thermotoga petrophila Thermotoga maritima -linkages andare storage polysaccharides. Ivory nutisasource ofmannan. Podospora anserina Podospora anserina Thermobifida fusca Cellvibrio japonicus Cellvibrio japonicus Source Organism Source Organism Bacillus circulans Streptomyces sp. Streptomyces sp. Bacillus subtilis Escherichia coli Bacillus sp. CBM35-GH26- CBM10-GH134 Architecture CBM35-GH26 Architecture CBM35-GH26 GH5-CBM32 CBM23-GH5 GH5-GH5 CBM35 GH134 CAZ GH113 GH24 GH76 GH76 GH76 GH26 GH26 GH26 CAZ GH26 GH26 GH26 GH5 GH5 GH5 GH5 GH5 GH5 GH5 GH5 GH5 GH5 -D-mannosidic linkages in mannans, galactomannans y y 3.2.1.101 3.2.1.101 3.2.1.101 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.78 3.2.1.17 3.2.1.4 EC EC Optimum Optimum Optimum Optimum 5,0-6,0 6,5-8,0 5,0-6,0 6,0-8,0 4,0-6,0 3,0-6,0 4,5-6,5 7,0-8,0 6,0-7,0 5,0-7,0 5,0-7,1 6,0 6,0 pH 5,0 5,0 5,0 6,5 9,5 pH 7,0 7,0 5,5 7,0 7,0 7,5 7,0 7,0 7,0 7,0 7,0 Temperature (ºC) Temperature (ºC) Optimum Optimum 81-93 50 60 60 80 60 90 40 40 50 50 65 30 25 65 45 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 vial vial 0,5 0,5 0,5 0,5 0,5 0,5 1,8 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 oligosaccharides orcarbohydrates. β β α α Catalogue Catalogue -Mannosidases are enzymes withactivity exo-1,4-β -Mannosidases are enzymes thatparticipate inthehydrolysis ofterminal, non-reducing α Number Number CZ0694 CZ0320 CZ0440 CZ0950 CZ0027 CZ0568 CZ0700 CZ0332 CZ0556 CZ0810 CZ0239 CZ0109 CZ0233 CZ0140 CZ1082 CZ0166 CZ0168 CZ0148 CZ0144 CZ0149 CZ0139 CZ0154 CZ0145 CZ0167 CZ0175 CZ0172 CZ0141 CZ0151 -Mannosidases -Mannosidases 1,3-α-Mannosidase 92A α-Mannosidase 125A α-Mannosidase 125A α-Mannosidase 125A α-Mannosidase 92M β-Mannosidase 26A α-Mannosidase 99B α-Mannosidase 92H α-Mannosidase 92D α-Mannosidase 38A α-Mannosidase 92A α-Mannosidase 92O α-Mannosidase 92G α-Mannosidase 38A α-Mannosidase 92C α-Mannosidase 92B α-Mannosidase 92E α-Mannosidase 92S α-Mannosidase 92F α-Mannosidase 47A α-Mannosidase 92L α-Mannosidase 92J α-Mannosidase 92I β-Mannosidase 5A β-Mannosidase 2A β-Mannosidase 2A β-Mannosidase 1A β-Mannosidase 1B Enzyme Enzyme Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Streptococcus pneumoniae Streptococcus pyogenes Clostridium perfringens Thermotoga maritima Pyrococcus furiosus Pyrococcus furiosus Bacteroides fragilis Source Organism Source Organism Cellvibrio mixtus Caulobacter sp. -mannosidase thatremoves mannose residues from thenon-reducing endsof Architecture Architecture GH92-GH92 GH125 GH125 GH125 CAZ GH26 GH99 CAZ GH92 GH92 GH92 GH38 GH92 GH92 GH92 GH92 GH92 GH92 GH92 GH92 GH38 GH92 GH92 GH92 GH47 GH5 GH2 GH2 GH1 GH1 y y 3.2.1.113 and 3.2.1.100 3.2.1.130 3.2.1.113 3.2.1.25 3.2.1.25 3.2.1.25 3.2.1.25 3.2.1.113 3.2.1.25 3.2.1.113 3.2.1.113 3.2.1.24 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- -D-mannose residues inα EC EC Optimum Optimum Optimum Optimum 6,5-7,5 6,5-7,5 5,5-7,5 5,0 5,6 pH 6,5 6,5 6,5 6,5 6,5 6,5 pH 6,5 7,0 7,0 6,5 7,0 7,0 7,0 7,0 7,0 7,0 7,0 7,0 7,5 7,5 7,5 7,4 7,4 Temperature (ºC) Temperature (ºC) Optimum Optimum -D-mannosides. 90 50 95 37 37 37 25 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 0,25 0,25 0,25 vial vial 0,5 0,5 0,5 0,5 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 31 cazymes 32 NZYTech 2021 of oligosaccharides. Oligosaccharide reducing-end xylanases are enzymes that participate in the hydrolysis of 1,4-β Oligosaccharide reducing-end xylanases residues inpolygalacturonan, asignificant carbohydrate component ofthepectinnetwork ofplant cell walls. Polygalacturonases, also known as pectins depolymerases, are enzymes thathydrolyze the1,4-α-glycosidic bondsbetween galacturonic acid hydrobond intheMurNAc residue. N-acetylmuramic acid(MurNAc) andN-acetylglucosamine (GlcNAc) residues inpeptidoglycans withtheconcomitant formation ofa1,6-an - Peptidoglycan lytic exotransglycosylases are enzymes thatparticipate intheexolytic cleavage ofthe1-4-β Peptidoglycan lytic exotransglycosylases nose-1-phosphate (M1P) andD-glucose (Glc) inaninverting manner. Mannosylglucose phosphorylases are enzymes thatconvert D-mannopyranosyl-1,4-β Mannosylglucose phosphorylases Poly Catalogue Catalogue Catalogue Catalogue Number Number Number Number CZ0400 CZ0880 CZ0688 CZ0696 CZ0385 CZ0246 CZ0244 CZ0687 CZ0794 CZ0245 CZ0773 CZ0816 CZ1066 CZ0193 CZ1067 CZ0116 galacturonases Mannosylglucose phosphorylase Mannosylglucose phosphorylase Peptidoglycan lytic exotransgly Peptidoglycan lytic exotransgly Peptidoglycan lytic exotransgly Peptidoglycan lytic exotransgly Oligosaccharide reducing-end Oligosaccharide reducing-end Endopolygalacturonase 28A Endopolygalacturonase 28A Polygalacturonosidase 28A Polygalacturonosidase 28B 28A Polygalacturonase 28A Polygalacturonase 28B Polygalacturonase 4A cosylase 102A cosylase 103A cosylase 23A cosylase 23A xylanase 8A xylanase 8A Enzyme Enzyme Enzyme Enzyme 130A 130A - - - - Bacteroides thetaiotaomicron Bifidobacterium adolescentis Zobellia galactanivorans Zobellia galactanivorans Thermotoga maritima Ruminococcus albus Bacillus halodurans Bacteroides fragilis Source Organism Source Organism Source Organism Source Organism Dickeya dadantii Dickeya dadantii Dickeya dadantii Bacillus subtilis Bacillus subtilis Escherichia coli Escherichia coli Escherichia coli Architecture Architecture Architecture Architecture GH130 GH130 GH102 GH103 CAZ CAZ GH28 GH28 CAZ CAZ GH28 GH28 GH28 GH28 GH28 GH23 GH23 GH8 GH8 GH4 y y y y 2.4.1.281 2.4.1.281 3.2.1.156 3.2.1.156 4.2.2.n1 4.2.2.n1 4.2.2.n1 4.2.2.n1 3.2.1.82 3.2.1.82 3.2.1.67 3.2.1.67 3.2.1.67 3.2.1.67 3.2.1.15 3.2.1.15 -D-glucopyranose (Man-Glc) to D-man- EC EC EC EC -xylose residues from the reducing end Optimum Optimum Optimum Optimum Optimum Optimum 4,5-10,5 6,0-6,5 4,0-4,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 7,0-7,5 7,2-7,8 6,0 6,0 6,0 8,0 6,0 6,0 pH pH pH pH 7,0 -glycosidic linkage between Temperature (ºC) Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum Optimum 37-40 40 40 40 50 30 95 37 37 37 37 37 37 37 37 37 mg/ mg/ mg/ mg/ 0,25 0,25 0,25 0,25 0,25 0,25 vial vial vial vial 0,5 0,5 1 1 1 1 1 1 1 1 of therepeating disaccharide: 1,4-α-D-GalpA-1,2-α-L-Rhap-1. lacturonate at the reducing end. Rhamnogalacturonans are agroup ofclosely related cell wall pecticpolysaccharides thatcontain abackbone Rhamnogalacturonases are enzymes thatparticipate intheendo-hydrolysis ofrhamnogalacturonans releasing oligosaccharides withβ-D-ga- Rhamnogalacturonases in Pullulanases are enzymes thatparticipate inthehydrolysis of1,6-α P backbone ofporphyran consists largely (~70%) of(1->3)-linked β Porphyranases are enzymes thatperform thehydrolysis ofβ Porphyranases Catalogue Catalogue Catalogue Number Number Number CZ0680 CZ0884 CZ0883 CZ0395 CZ0790 CZ0867 CZ0762 CZ0734 CZ0770 CZ0779 CZ0724 CZ0735 CZ0194 CZ0723 CZ1044 CZ0129 CZ0737 CZ0221 CZ0179 CZ1061 α CZ1012 ullulanases - andβ -limit dextrins ofamylopectin andglycogen. Unsaturated rhamnogalacturonyl Unsaturated rhamnogalacturonyl Unsaturated rhamnogalacturonyl Unsaturated rhamnogalacturonyl Unsaturated rhamnogalacturonyl α-Rhamnosidase 78B &Kdo α-Rhamnosidase 106A α-Rhamnosidase 106A α-Rhamnosidase 145A α-Rhamnosidase 78A α-Rhamnosidase 78A α-Rhamnosidase 78A α-Rhamnosidase 78B α-Rhamnosidase 78C β-Porphyranase 86A β-Porphyranase 16A β-Porphyranase 16B β-Porphyranase 16C 13A Neopullulanase 13A 13A hydrolase 105A hydrolase 105A hydrolase 105D hydrolase 105B hydrolase 105C hydrolase 33A Enzyme Enzyme Enzyme Geobacillus stearothermophilus Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Sphingomonas paucimobilis Streptomyces avermitilis Zobellia galactanivorans Zobellia galactanivorans Thermotoga maritima Bacteroides uniformis Bacteroides plebeius Bacteroides plebeius Source Organism Source Organism Source Organism Bacillus subtilis -D-galactopyranose-1,4-α -D-galactopyranose, followed by (1->4)-linked α -D-glucosidic linkages inpullulan, amylopectin andglycogen, and CBM67-GH78- Architecture Architecture Architecture CBM67-GH78 CBM67-GH78 CBM34-GH13 GH106 GH106 GH105 GH105 GH105 GH105 GH105 GH145 GH86 CAZ CAZ CAZ GH78 GH78 GH78 GH33 GH16 GH16 GH16 GH13 GH13 y y y -L-galactopyranose-6-sulfate linkages inporphyran. The 3.2.1.40 and 3.2.1.135 3.2.1.135 3.2.1.178 3.2.1.178 3.2.1.178 3.2.1.178 3.2.1.172 3.2.1.172 3.2.1.172 3.2.1.172 3.2.1.40 3.2.1.40 3.2.1.40 3.2.1.40 3.2.1.40 3.2.1.41 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- EC EC EC Optimum Optimum Optimum Optimum 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 8,0 4,0 6,0 5,0 5,9 pH pH pH 7,0 7,0 7,0 7,8 7,5 -L-galactopyranose-6-sulphate. Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 90 40 30 30 30 30 45 55 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ mg/ 0,25 0,25 vial vial vial 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 33 cazymes 34 NZYTech 2021 two α-glucosetwo units, to glucose. Trehalases are enzymes thatcatalyze theconversion oftrehalose, anatural α-linked disaccharide formed by anα,α-1,1-glucoside bondbetween Trehalases nic acidandsynthetic substrates. Sialidases may actaspathogenic factors inmicrobial infections. Sialidases hydrolyze 2,3-α,2,6- Sialidases Rhamnogalacturonases (CONT.) Catalogue Catalogue Catalogue Number Number Number CZ0990 CZ0544 CZ0936 CZ0559 CZ0870 CZ0228 CZ0226 CZ0769 CZ0703 CZ0532 CZ0533 CZ0765 CZ0673 CZ0237 CZ0481 CZ0797 CZ1049 CZ0231 CZ0871 CZ1076 CZ1075 CZ1071 Malto-oligosyltrehalose synthase 13A Malto-oligosyltrehalose trehalohy Malto-oligosyltrehalose trehalohy Malto-oligosyltrehalose trehalohy Unsaturated rhamnogalacturonyl Unsaturated rhamnogalacturonyl Unsaturated rhamnogalacturonyl α-Trehalose phosphorylase 65A α-Phosphotrehalase 13A α-Phosphotrehalase 13A α-Phosphotrehalase 13A Trehalose synthase 13A Trehalose synthase 13A hydrolase 105A hydrolase 105A hydrolase 105B Trehalase 37A Trehalase 37B Trehalase 15A Sialidase 33D Sialidase 33A Sialidase 33A Sialidase 33A Sialidase 33A Sialidase 33B drolase 13A drolase 13A drolase 13A Enzyme Enzyme Enzyme α and2,8-α-glycosidic linkages ofterminal sialic residues inoligosaccharides, glycoproteins, glycolipids, colomi- - - - Thermoanaerobacter brockii Streptococcus pneumoniae Streptococcus pneumoniae Mycobacterium smegmatis Mycobacterium smegmatis Lactobacillus acidophilus Zobellia galactanivorans Clostridium perfringens Clostridium perfringens Clostridium perfringens Sulfolobus solfataricus Bacillus licheniformis Nostoc punctiforme Deinococcus deserti Bacteroides fragilis Source Organism Source Organism Source Organism Arthrobacter sp. Mariniflexile sp. Mariniflexile sp. Escherichia coli Escherichia coli Escherichia coli Rhizobium sp. CBM40-GH33a Architecture Architecture Architecture CBM48-GH13 CBM48-GH13 CBM48-GH13 -UNK-GH33b GH33a-UNK- GH33b GH105 GH105 GH105 CAZ CAZ CAZ GH65 GH33 GH33 GH33 GH33 GH37 GH37 GH15 GH13 GH13 GH13 GH13 GH13 GH13 y y y 5.4.99.16 5.4.99.16 5.4.99.15 3.2.1.172 3.2.1.172 3.2.1.172 3.2.1.141 3.2.1.141 3.2.1.141 2.4.1.64 3.2.1.28 3.2.1.28 3.2.1.28 3.2.1.93 3.2.1.93 3.2.1.93 3.2.1.18 3.2.1.18 3.2.1.18 3.2.1.18 3.2.1.18 3.2.1.18 EC EC EC Optimum Optimum Optimum Optimum 6,0-9,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 7,0-7,5 8,0 6,0 6,0 9,0 5,0 6,5 pH pH pH 7,0 7,0 7,5 7,5 7,5 7,5 7,4 7,2 7,1 Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 80 40 60 40 30 30 45 25 25 25 25 25 37 37 37 37 37 37 37 37 37 37 mg/ mg/ mg/ 0,25 0,25 0,25 0,25 0,25 0,25 0,25 0,25 vial vial vial 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 gosaccharides produced from hydrolysis are considered as “functional food” ordietary fibers. celluloses found inplant cell walls andsome algae. Xylans are usually decorated withacetyl, arabinose andglucuronic acidsidechains. Xylooli - Xylanases are enzymes thatdegrade thelinear polysaccharide 1,4-β-xylan into xylo-oligosaccharides. Xylan isoneofthemost abundanthemi- X terminus andthesaccharide linked to theresidue. Catalyze thehydrolysis oftheglycosidic bondinanunsaturated saccharide between theunsaturated glucuronyl residue atthenon-reducing Δ Catalogue Catalogue Number Number CZ0006 CZ0004 CZ0005 CZ0002 CZ0003 CZ0856 CZ0954 CZ0845 CZ0204 CZ0939 CZ0290 CZ0007 CZ0443 CZ0022 CZ0382 CZ0383 CZ0001 CZ0325 CZ0276 CZ1068 CZ0275 CZ0051 CZ1065 CZ0185 CZ0107 ylanases -4,5-Unsaturated Δ-4,5-Unsaturated β-glucuronyl Δ-4,5-Unsaturated β-glucuronyl Δ-4,5-Unsaturated β-glucuronyl Δ-4,5-Unsaturated β-glucuronyl Δ-4,5-unsaturated β-glucuronyl Δ-4,5-unsaturated β-glucuronyl Δ-4,5-unsaturated β-glucuronyl Xylanase 11A&Acetyl xylan hydrolase 105A hydrolase 105A hydrolase 88A hydrolase 88A hydrolase 88A hydrolase 88A hydrolase 88B Xylanase 10A Xylanase 10D Xylanase 10D Xylanase 10A Xylanase 10B Xylanase 10B Xylanase 10A Xylanase 10B Xylanase 10B Xylanase 10C Xylanase 10C Xylanase 11A Xylanase 11A Xylanase 11A Xylanase 11A Xylanase 11A Xylanase 11A esterase 4A Enzyme Enzyme β Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron -glucuronyl hydrolases Ruminococcus flavefaciens Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Zobellia galactanivorans Zobellia galactanivorans Nonlabens ulvanivorans Clostridium perfringens Pedobacter heparinus Podospora anserina Thermobifida fusca Thermobifida fusca Cellvibrio japonicus Caldicellulosiruptor Cellvibrio japonicus Caldicellulosiruptor Source Organism Source Organism Cellvibrio mixtus Bacillus subtilis saccharolyticus saccharolyticus GH11-CBM6-CE4 CBM22-GH10- Architecture Architecture GH10-CBM22 CBM22-GH10 CBM22-GH10 GH11-CBM6 GH11-CBM1 CBM22 GH105 GH105 GH88 GH88 GH88 GH88 GH88 CAZ CAZ GH10 GH10 GH10 GH10 GH10 GH10 GH10 GH11 GH11 GH11 GH11 y y 3.2.1.8 and 3.1.1.72 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- EC EC Optimum Optimum Optimum 4,5-8,0 4,5-8,0 4,5-8,0 4,0-11,0 5,0-6,0 5,0-8,0 5,0-8,0 6,0-9,0 5,0-8,0 5,0-8,0 5,0-8,0 6,5-9,0 5,5-6,0 5,0-7,5 7,0-8,0 7,0-8,0 5,0-7,0 6,5-7,5 6,5-7,5 6,5-7,5 6,0 6,6 6,5 6,5 pH pH 7,5 Temperature (ºC) Temperature (ºC) Optimum Optimum 20-55 70-85 60 40 30 50 50 50 50 65 65 65 65 65 65 65 70 37 37 37 37 37 37 37 37 mg/ mg/ 0,75 vial vial 0,5 0,5 0,5 1,5 1,5 1,5 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 35 cazymes 36 NZYTech 2021 X Catalogue Number CZ0900 CZ0988 CZ0996 CZ0495 CZ0995 CZ0945 CZ0563 CZ0987 CZ0433 CZ0746 CZ0975 CZ1000 CZ0401 CZ0918 CZ0491 CZ0915 CZ1030 CZ0912 CZ1028 CZ1063 CZ0718 CZ0716 CZ1079 CZ0911 CZ1051 ylanases ( Xylanase 10BA &Arabinofuranosi- Xylanase 98A Xylanase 98A Xylanase 30A Xylanase 30B Xylanase 10A Xylanase 10A Xylanase 10A Xylanase 10A Xylanase 10A Xylanase 10A Xylanase 10A Xylanase 10A Xylanase 10A Xylanase 10B Xylanase 10A Xylanase 10A Xylanase 10B Xylanase 10B Xylanase 11A Xylanase 11A Xylanase 11A Xylanase 11B Xylanase 8A Xylanase 5A dase 43B Enzyme cont .) Geobacillus stearothermophilus Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Ruminococcus champanellensis Alicyclobacillus acidocaldarius Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Clostridium papyrosolvens Clostridium papyrosolvens Zobellia galactanivorans Thermotoga maritima Thermotoga maritima Rhodopirellula baltica Bacteroides eggerthii Bacillus halodurans Thermobifida fusca Thermobifida fusca Cellvibrio japonicus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Source Organism CBM22-GH10-Doc doc-CBM22-CE4 CBM22-GH43- GH10-CBM22- CBM22-GH10- CBM22-GH10- GH98-CBM35 GH98-CBM35 Architecture GH30-CBM22 GH8-doc-doc GH11-CBM22- CBM22-GH10 GH10-CBM2 CBM22-Doc GH11-CBM2 GH10-doc CBM6 CAZ GH30 GH10 GH10 GH10 GH10 GH10 GH10 GH10 GH10 GH10 GH11 GH11 GH5 y 3.2.1.8 and 3.2.1.55 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 3.2.1.8 EC Optimum Optimum 9,0-10,0 5,0-6,0 5,0-6,0 6,0-8,0 5,0-8,0 5,5-6,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,0 6,0 6,0 6,0 6,0 6,0 5,8 6,5 6,5 pH 6,2 7,0 7,2 7,2 7,2 Temperature (ºC) Optimum 55-60 65-75 90 50 65 25 25 75 37 37 37 37 37 75 37 37 37 37 37 37 37 37 37 37 37 mg/ 0,25 0,25 0,25 vial 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 non-reducing termini ofthecarbohydrate. Xylosidases are exo-acting enzymes thatactonpoly oroligosaccharides to remove successive D-xylose residues from thereducing or X 1,4-β-glucan active enzymes may non-specifically cleave xyloglucan. Xyloglucanases are enzymes thatcleave the1,4-β-glucan backbone ofxyloglucan butdonotattack cellulosic substrates. The majority of X Catalogue Catalogue Number Number CZ0888 CZ0866 CZ0998 CZ0488 CZ0486 CZ0206 CZ0944 CZ0389 CZ0462 CZ0706 CZ0536 CZ0789 CZ0207 CZ0535 CZ0522 CZ0957 CZ0910 CZ0776 CZ0021 CZ0310 CZ1062 CZ0971 CZ0715 CZ1010 ylosidases yloglucanases Xyloglucanase 44A Xyloglucanase 74A Xyloglucanase 74A Xyloglucanase 74A Xyloglucanase 74A Xyloglucanase 9A Xyloglucanase 5A β-Xylosidase 43B α-Xylosidase 31A Xylosidase 120A Xylosidase 120B Xylosidase 39A Xylosidase 43A Xylosidase 43A Xylosidase 43A Xylosidase 43A Xylosidase 43A Xylosidase 43B Xylosidase 43A Xylosidase 3A Xylosidase 3A Xylosidase 3A Xylosidase 3A Xylosidase 3A Enzyme Enzyme Thermoanaerobacterium saccha- Ruminococcus champanellensis Ruminococcus champanellensis Pseudothermotoga thermarum Bifidobacterium adolescentis Bifidobacterium adolescentis Acidothermus cellulolyticus Clostridium thermocellum Clostridium cellulolyticum Clostridium cellulolyticum Zobellia galactanivorans Clostridium stercorarium Clostridium stercorarium Sulfolobus solfataricus Thermotoga maritima Paenibacillus pabuli Thermobifida fusca Cellvibrio japonicus Cellvibrio japonicus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Bacteroides ovatus Source Organism Source Organism Prevotella bryantii rolyticum GH74-doc-doc GH44-CBM76 Architecture Architecture CBM30-GH9- doc-doc GH120 GH120 CAZ CAZ GH39 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH43 GH74 GH74 GH74 GH31 GH5 GH3 GH3 GH3 GH3 GH3 y y 3.2.1.37 and 3.2.1.37 and 3.2.1.177 3.2.1.151 3.2.1.151 3.2.1.151 3.2.1.151 3.2.1.151 3.2.1.151 3.2.1.56 3.2.1.55 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.37 3.2.1.- EC EC Optimum Optimum Optimum 5,0-6,0 6,0-8,0 4,0-5,0 5,0-7,0 5,5-6,0 5,5-6,5 6,5-7,5 6,5-7,5 6,0 6,0 6,0 6,0 6,0 6,8 6,5 5,5 pH pH 5,5 3,5 7,0 7,0 7,5 7,2 7,2 7,2 Temperature (ºC) Temperature (ºC) Optimum Optimum 55-60 75-85 75-83 100 60 60 80 30 65 45 55 25 25 25 75 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 0,25 0,25 vial vial 1,75 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 37 cazymes 38 NZYTech 2021 Below are arange ofenzymes thatare notinsufficientnumberto beclassified asaseparate group. O Catalogue Number CZ0800 CZ0809 CZ0848 CZ0402 CZ0802 CZ0884 CZ0803 CZ0388 CZ0964 CZ0820 CZ0930 CZ0956 CZ0929 CZ0796 CZ0932 CZ0979 CZ0872 CZ0819 CZ0787 CZ0518 CZ0941 CZ0815 CZ1003 CZ0521 CZ0531 CZ1025 ther A Glucan 1,4-α-maltohexaosidase 13A α-1,4-Glucan branching enzyme 13A Dha hydrolase 143A &β-L-Arabino- Rhamnogalacturonan rhamnohy Mannosylglycerate hydrolase 63A β 1,3- Oligosaccharide 4-α-D-glucosyl Cyclodextrin glucanotransferase Cellodextrin phosphorylase 94A 3-O-α-D-Glucosyl-L-rhamnose Nigerose phosphorylase 65A α-Rhamnosidase 78B &Kdo Sucrose glucosylmutase 13A 5A Sucrose phosphorylase 13 A Aceric acidhydrolase 127A 13A Aceric acidhydrolase 127B β-Mannooligosaccharide Sulfoquinovosidase 31A -Glucanosyltransglucosidase 3A β-L-Arabinosidase 27A Endo-apiosidase 140A ctivities phosphorylase 130A Dha hydrolase 143A phosphorylase 65A furanosidase 142A Amylosucrase 13A Inulosucrase 68A 31A hydrolase 33A drolase 106A Enzyme 13A 17A - - Geobacillus stearothermophilus Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Clostridium phytofermentans Clostridium phytofermentans Bifidobacterium adolescentis Rubrobacter radiotolerans Neisseria polysaccharea Pseudomonas putida Lactobacillus gasseri Ruminococcus albus Ruminococcus albus Bacillus halodurans Cellvibrio japonicus Source Organism Erwinia rhapontici Erwinia Paenibacillus sp. Paenibacillus sp. Rhodococcus sp. Escherichia coli Escherichia coli Bacillus sp. CBM67-GH78- Architecture GH143-GH142 CBM48-GH13 GH13-CBM20 CBM34-GH13 GH106 GH140 GH130 GH143 GH127 GH127 GH68 CAZ GH94 GH65 GH65 GH63 GH33 GH27 GH13 GH13 GH31 GH13 GH31 GH13 GH17 GH5 GH3 y 3.2.1.40 and 3.2.1.- and 2.4.1.282 2.4.1.279 5.4.99.11 2.4.1.319 3.2.1.185 3.2.1.123 3.2.1.170 3.2.1.174 2.4.1.161 3.2.1.98 3.2.1.88 3.2.1.54 2.4.1.49 3.2.1.45 2.4.1.18 2.4.1.19 2.4.1.9 2.4.1.4 2.4.1.7 2.4.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- 3.2.1.- EC Optimum Optimum 5,0-6,0 4,5-6,0 6,5-7,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,0 5,0 4,0 5,0 6,0 9,0 6,5 6,5 6,5 pH 6,5 6,5 7,0 7,0 7,0 7,0 Temperature (ºC) Optimum 40 40 50 30 40 30 30 50 30 30 25 25 55 22 37 37 37 37 37 37 37 37 37 37 37 37 mg/ 0,25 0,25 0,25 0,25 0,25 0,25 vial 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 A A Ferulo Diace ce ce tyl x tylglucosamin e d tylchitobiose d yl e ylan e sterase sterase s

ace ace s

* Structure ofFeruloyl esterase 1Afrom Clostridium thermocellum (PDB1GKL), #CZ0031, pg41 tylase tylase s s

Pe Pe Glucuronyl e ctin me ctin ace thyle tyl e sterase sterase sterase s

s s

carbohydrate esterases 39 cazymes 40 NZYTech 2021 Acetylglucosamine deacetylases are enzymes thatdeacetylate carbohydrates containing N-acetyl-D-glucosamine. A ed xylan andxylo-oligosaccharides. Acety xylan esterases hydrolyze theester linkages oftheacetyl groups inposition 2and/or 3ofthexylose moietiesofnatural acetylat A Catalogue Catalogue Number Number CZ0699 CZ0683 CZ0949 CZ0034 CZ0520 CZ0429 CZ0685 CZ0052 CZ0682 CZ0032 CZ0033 CZ0436 CZ0224 CZ0435 CZ0242 CZ0010 CZ0190 CZ0785 CZ0051 CZ0327 CZ0210 CZ0170 CZ0134 CZ0133 CZ0241 CZ0132 cetylglucosamine deacetylases cetyl xylan esterases Peptidoglycan N-Acetylglucosamine Cellulase 5C&Acetyl xylan esterase 2A N-acetylglucosamine 6-phosphate Peptidoglycan N-acetylglucosami- N-Acetylglucosaminylphosphati- N-Acetylglucosaminylphosphati- N-acetylglucosamine 6-phosphate UDP-acetylglucosamine deace - UDP-acetylglucosamine deace- Xylanase 11A&Acetyl xylan dylinositol deacetylase 14A dylinositol deacetylase 14A Acetyl xylan esterase 12A Acetyl xylan esterase 15A Acetyl xylan esterase 12A Acetyl xylan esterase 6A Acetyl xylan esterase 6B Acetyl xylan esterase 4A Acetyl xylan esterase 4A Acetyl xylan esterase 4A Acetyl xylan esterase 2A Acetyl xylan esterase 3A Acetyl xylan esterase 3B Acetyl xylan esterase 7A Acetyl xylan esterase 2B Acetyl xylan esterase 7A Acetyl xylan esterase 2C Acetyl xylan esterase 3A ne deacetylase 4A deacetylase 4A deacetylase 9B deacetylase 9A esterase 4A tylase 11A tylase 11A Enzyme Enzyme Mycobacterium tuberculosis Streptococcus pneumoniae Mycobacterium smegmatis Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Pseudomonas aeruginosa Fibrobacter succinogenes Fibrobacter succinogenes Thermotoga maritima Bacillus halodurans Cellvibrio japonicus Cellvibrio japonicus Cellvibrio japonicus Source Organism Source Organism Bacillus subtilis Bacillus subtilis Bacillus subtilis Bacillus subtilis Escherichia coli Escherichia coli GH11-CBM6-CE4 Architecture Architecture CBM6-CE4 GH5-CE2 CAZ CAZ CE14 CE14 CE15 CE12 CE12 CE11 CE11 CE9 CE9 CE4 CE4 CE4 CE6 CE6 CE4 CE2 CE3 CE2 CE3 CE3 CE2 CE7 CE7 y y 3.1.1.72 and 3.2.1.8 and 3.2.1.4 and 3.5.1.89 3.5.1.89 3.5.1.25 3.5.1.25 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.72 3.1.1.41 3.5.1.- 3.5.1.- 3.5.1.- 3.5.1.- EC EC Optimum Optimum Optimum Optimum 6,5-8,0 4,5-8,0 4,5-8,0 6,0-8,0 6,5-8,0 5,0-7,5 8,0 8,0 6,0 6,0 6,8 6,8 6,5 pH 8,5 pH 7,0 7,0 7,0 7,0 7,0 7,5 7,5 7,5 7,5 7,5 7,5 7,3 Temperature (ºC) Temperature (ºC) Optimum Optimum 100 60 40 30 30 30 50 40 50 65 65 65 45 35 35 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 0,25 0,75 vial vial 0,5 2,5 1,5 4 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 - maceutical industries. of lignin from carbohydrates, butalso to synthesize abroad range ofnovel bioactive components for use infood, cosmetic andphar usually arabinose in “natural” substrates. These enzymes have theability notonly to deconstruct plant biomass, through therelease Feruloyl esterases catalyze thehydrolysis ofthe4-hydroxy-3-methoxycinnamoyl (feruloyl) group from anesterified sugar, whichis Feruloyl esterases Diacetylchitobiose deacetylases are enzymes thatdeacetylate diacetylchitobiose. Diacetylchitobiose deacetylases Catalogue Catalogue Number Number CZ0600 CZ0580 CZ0405 CZ0590 CZ0586 CZ0588 CZ0584 CZ0589 CZ0439 CZ0596 CZ0598 CZ0585 CZ0594 CZ0599 CZ0582 CZ0583 CZ0595 CZ0592 CZ0593 CZ0587 CZ0597 CZ0601 CZ0031 CZ0581 CZ0591 CZ0143 Diacetylchitobiose deacetylase 14A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1B Feruloyl esterase 1B Feruloyl esterase 1B Feruloyl esterase 1C Feruloyl esterase 1A Enzyme Enzyme Clostridium saccharoperbutylace- Clostridium saccharoperbutylace- Lactobacillus fabifermentans Uncultured rumenbacterium Paenibacillus mucilaginosus Streptococcus pneumoniae Clostridium thermocellum Clostridium thermocellum Uncultured soil bacterium Clostridium cellulovorans Zunongwangia profunda Clostridium stercorarium Streptococcus pyogenes Acetivibrio cellulolyticus Sphingomonas wittichii Butyrivibrio fibrisolvens Lactobacillus johnsonii Lactobacillus johnsonii Lactobacillus buchneri Mycobacterium avium Lactobacillus reuteriI Ruminococcus albus Melioribacter roseus Source Organism Source Organism Dickeya dadantii Opitutus terrae Bacillus cereus tonicum tonicum Architecture Architecture CAZ CAZ CE14 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 y y 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.5.1.- EC EC Optimum Optimum Optimum 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 4,0-7,0 4,0-7,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 8,0 pH pH 7,0 Temperature (ºC) Temperature (ºC) Optimum Optimum 60 60 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ 0,25 0,25 vial vial 0,5 0,5 0,5 0,5 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 - 41 cazymes 42 NZYTech 2021 Feruloyl esterases (CONT.) Catalogue Number CZ0606 CZ0608 CZ0609 CZ0604 CZ0605 CZ0602 CZ0603 CZ0620 CZ0607 CZ0630 CZ0626 CZ0628 CZ0629 CZ0624 CZ0636 CZ0625 CZ0634 CZ0623 CZ0622 CZ0635 CZ0632 CZ0633 CZ0610 CZ0627 CZ0616 CZ0618 CZ0619 CZ0614 CZ0615 CZ0613 CZ0612 CZ0621 CZ0631 CZ0617 CZ0611 Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1C Feruloyl esterase 1C Enzyme Clostridium saccharoperbutylace- Lactobacillus kefiranofaciens Thermobacillus xylanilyticus Mycobacterium mageritense Mycobacterium mageritense Amycolatopsis mediterranei Mycobacterium tuberculosis Butyrivibrio proteoclasticus Butyrivibrio proteoclasticus Mycobacterium smegmatis Bacteroides cellulosilyticus Cellulosilyticum ruminicola Lactobacillus amylovorus Legionella pneumophila Mycobacterium kansasii Lactobacillus helveticus Paenibacillus polymyxa Butyrivibrio fibrisolvens Butyrivibrio fibrisolvens Streptococcus sobrinus Klebsiella pneumoniae Klebsiella pneumoniae Deinococcus gobiensis Streptococcus downei Uncultured bacterium Ruminococcu salbus Paenibacillus terrae Paenibacillus terrae Source Organism Thioalkalivibrio sp. Rhodococcus jostii Klebsiella oxytoca Actinomyces sp. Butyrivibrio sp. Butyrivibrio sp. Shigella boydii tonicum Architecture CAZ CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 y 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 EC Optimum Optimum 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 pH Temperature (ºC) Optimum 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ 0,25 0,25 0,25 0,25 vial 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Feruloyl esterases (CONT.) Catalogue Number CZ0640 CZ0660 CZ0646 CZ0648 CZ0644 CZ0650 CZ0649 CZ0638 CZ0639 CZ0666 CZ0645 CZ0668 CZ0664 CZ0642 CZ0656 CZ0658 CZ0654 CZ0643 CZ0669 CZ0659 CZ0665 CZ0655 CZ0662 CZ0663 CZ0652 CZ0653 CZ0647 CZ0637 CZ0670 CZ0667 CZ0657 CZ0641 CZ0661 CZ0651 CZ0671 Feruloyl esterase 1A Feruloyl esterase 1D Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1C Feruloyl esterase 1A Feruloyl esterase 1A Feruloyl esterase 1C Feruloyl esterase 1A Feruloyl esterase 1B Feruloyl esterase 1B Feruloyl esterase 1B Feruloyl esterase 1C Enzyme Thermoanaerobacter mathranii Lactobacillus kefiranofaciens Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium vanbaalenii Bacteroide reticulotermitis Pelagibacter halotolerans Acinetobacter baumannii Lactobacillus acidophilus Lactobacillus delbrueckii Bifidobacterium bifidum Bifidobacterium longum Lysinibacillus sphaericus Bifidobacterium longum Streptococcus sanguinis Lactobacillus crispatus Lactobacillus crispatus Ruminococcus callidus Bifidobacterium breve Uncultured bacterium Segniliparus rotundus Variovorax paradoxus Bacillus licheniformis Leuconostoc citreum Bacteroides plebeius Streptococcus criceti Eubacterium rectale Paenibacillus terrae Source Organism Ruegeria pomeroyi Spiribacter salinus Dickeya dadantii Butyrivibrio sp. Olsenella uli Frankia sp. Architecture CAZ CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 CE1 y 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 3.1.1.73 EC Optimum Optimum 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 7,0-9,0 pH Temperature (ºC) Optimum 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 mg/ 0,25 0,25 0,25 0,25 0,25 vial 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 43 cazymes 44 NZYTech 2021 Pectin acetyl esterases are enzymes thatparticipate intheremoval oftheacetyl sidechainsofpectin. Pectin acetyl esterases aromatic alcohols oflignin. Glucuronoyl esterases participate inthehydrolysis oftheester bondbetween 4-O-methyl-D-glucuronic acidresidues ofglucuronoxylans and Glucuronyl esterases Feruloyl esterases (CONT.) Pectin methylesterases are enzymes thatparticipate intheremoval ofthemethyl sidechainsofpectin. Pectin methylesterases Catalogue Catalogue Catalogue Catalogue Number Number Number Number CZ0036 CZ0699 CZ0037 CZ0672 CZ0576 CZ0578 CZ0579 CZ0574 CZ0575 CZ0573 CZ0814 CZ0577 CZ0727 CZ0216 CZ0813 CZ0791 CZ0721 CZ1013 Peptidoglycan N-Acetylglucosamine Pectin methylesterase NC Pectin Acetyl Esterase 12A Pectin acetyl esterase 12A Pectin methylesterase 8A Pectin methylesterase 8A Pectin methylesterase 8A Pectin acetyl esterase 12B Glucuronyl esterase 15A Glucuronyl esterase 15A Glucuronyl esterase 15A Glucuronyl esterase 15A Glucuronyl esterase 15A Glucuronyl esterase 15B RGI acetyl esterase NC Glucuronyl esterase 15B Feruloyl esterase 1B Feruloyl esterase 1B deacetylase 4A Enzyme Enzyme Enzyme Enzyme Ruminiclostridium thermocellum Caldicellulosiruptor kristjanssonii Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Clostridium cellobioparum Anaeromyces mucronatus Clostridium thermocellum Clostridium thermocellum Zobellia galactanivorans Teredinibacter turnerae Teredinibacter turnerae Dickeya chrysanthemi Prevotella ruminicola Source Organism Source Organism Source Organism Source Organism Dickeya dadantii Dickeya dadantii Bacillus subtilis Opitutus terrae Frankia sp. Architecture Architecture Architecture Architecture CE NC CE NC CAZ CAZ CAZ CAZ CE15 CE15 CE15 CE15 CE15 CE15 CE15 CE12 CE12 CE12 CE8 CE8 CE8 CE4 CE1 CE1 y y y y 3.1.1.73 3.1.1.73 3.1.1.11 3.1.1.11 3.1.1.11 3.1.1.11 3.5.1.- 3.1.1.- 3.1.1.- 3.1.1.- 3.1.1.- 3.1.1.- 3.1.1.- 3.1.1.- 3.1.1.- 3.1.1.- 3.1.1.- 3.1.1.- EC EC EC EC Optimum Optimum Optimum Optimum Optimum Optimum 8,0-9,0 8,0-9,0 8,0-9,0 6,0-8,5 6,0-8,5 7,0-9,0 6,5-7,5 8,0 6,0 6,0 6,0 6,0 6,0 6,0 6,0 pH pH pH pH 7,0 7,2 7,5 Temperature (ºC) Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum Optimum 45-70 40 50 30 65 65 37 37 37 37 37 37 37 37 37 37 37 37 mg/ mg/ mg/ mg/ 0,25 0,25 0,25 vial vial vial vial 0,5 0,5 0,5 0,5 0,5 1,5 2 1 1 1 1 1 1 1 1 Alginate l Hy Heparin l Chondroitin l Oligogalacturonate l Pe ctate l aluronate l y y y ase ase ase s s y y s

ase ase

s s

y ase s

* Structure ofOligogalacturonate 22A from Yersinia enterocolitica X Ul Rhamnogalacturonan l Pol Pe anthan l v ctin l an l y-α -guluronate l y y ase ase y ase s s

(PDB 3PE7), #CZ0726, pg47 s

y ase y s ase

s

polysaccharide lyases 45 cazymes 46 NZYTech 2021 found attached to proteins aspart ofaproteoglycan. sulfated (GAG) composed ofachainalternating sugars (N-acetylgalactosamine andglucuronic acid). Itisusually Chondroitin lyases are enzymes that catalyze thedegradation ofchondroitin by aβ ride distributed widely inthecell walls ofbrown algae, where through bindingwithwater itforms aviscous gum. Alginate lyases are enzymes thatcatalyze thedegradation ofalginate by aβ Alginate lyases C Catalogue Catalogue Number Number CZ0940 CZ0540 CZ0938 CZ0935 CZ0529 CZ0432 CZ0756 CZ0937 CZ1086 CZ0722 CZ1084 CZ1085 CZ0431 CZ0817 CZ0312 CZ1078 CZ0157 CZ1081 hondroitin lyases Chondroitin ABClyase 8B Chondroitin AC lyase 8A Chondroitin AC lyase 8A Chondroitin AC lyase 8A Oligoalginate lyase 15A Oligoalginate lyase 17A Oligoalginate lyase 7A Alginate lyase 14A Alginate lyase 15A Alginate lyase 5A Alginate lyase 7A Alginate lyase 7A Alginate lyase 7A Alginate lyase 7A Alginate lyase 7A Alginate lyase 7A Alginate lyase 7A Alginate lyase 7B Enzyme Enzyme Bacteroides thetaiotaomicron Saccharophagus degradans Saccharophagus degradans Pseudopedobacter saltans Pseudomonas aeruginosa Zobellia galactanivorans Zobellia galactanivorans Klebsiella pneumoniae Agrobacterium fabrum Rhodopirellula baltica Pedobacter heparinus Bacteroides stercoris Photobacterium sp. Source Organism Source Organism Sphingomonas sp. Sphingomonas sp. Agarivorans sp. Agarivorans sp Vibrio sp. Architecture Architecture -elimination mechanism. Alginate isananionicpolysaccha- CAZ CAZ PL14 PL15 PL15 PL17 PL8 PL8 PL8 PL8 PL5 PL7 PL7 PL7 PL7 PL7 PL7 PL7 PL7 PL7 y y -elimination mechanism. Chondroitin sulfate isa 4.2.2.20 4.2.2.5 4.2.2.5 4.2.2.5 4.2.2.3 4.2.2.3 4.2.2.3 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- EC EC Optimum Optimum Optimum 9,0-10,0 5,5-6,5 7,6-9,0 7,5-8,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 8,0 8,5 pH pH 7,0 7,0 7,6 7,5 7,5 7,2 Temperature (ºC) Temperature (ºC) Optimum Optimum 40 40 50 30 30 30 30 30 30 39 45 37 37 37 37 37 37 37 mg/ mg/ 0,25 vial vial 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 down to 3-(4-deoxy-β Hyaluronate lyases are enzymes thatcleave hyaluronate chainsataβ Hyaluronate lyases cleavage ofpectate, yielding oligosaccharides with4-deoxy-α Pectate lyases are enzymes involved inthemaceration andsoft rotting ofplant tissue. Pectate lyases are responsible for theeliminative Pectate lyases Oligogalacturonate lyases are enzymes thatparticipate intheeliminative cleavage of4-(4-deoxy-β Oligogalacturonate lyases gluc-4-enuronosyl groups attheirnon-reducing ends. ronate residues and1,4-α Heparin lyases are enzymes thatparticipate intheeliminative cleavage ofpolysaccharides containing 1,4-linked D-glucuronate orL-idu- Heparin lyases Catalogue Catalogue Catalogue Catalogue Number Number Number Number CZ0208 CZ0038 CZ0039 CZ0240 CZ0250 CZ0248 CZ0934 CZ0726 CZ0766 CZ0981 CZ0857 CZ0801 CZ0247 CZ0130 CZ0251 CZ0158 CZ0159 CZ0131 Oligogalacturonate lyase 22A Oligogalacturonate lyase 22A Heparin-sulfate lyase 12A Heparin-sulfate lyase 12A Heparin-sulfate lyase 12B Hyaluronate lyase 8A Exo-pectate lyase 2A Pectate lyase 10A Heparin lyase 15A Heparin lyase 13A Heparin lyase 21A Pectate lyase 9A Pectate lyase 9A Pectate lyase 3C Pectate lyase 1A Pectate lyase 1A Pectate lyase 1A Pectate lyase 1A -D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine. Enzyme Enzyme Enzyme Enzyme -linked 2-sulfoamino-2- deoxy-6-sulfo-D-glucose residues to give oligosaccharides withterminal 4-deoxy-α Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Streptococcus pneumoniae Clostridium thermocellum Clostridium thermocellum Thermotoga maritima Yersinia enterocolitica Pedobacter heparinus Bacteroides stercoris Cellvibrio japonicus Source Organism Source Organism Source Organism Source Organism Dickeya dadantii Dickeya dadantii Dickeya dadantii Dickeya dadantii Bacillus subtilis Bacillus subtilis -D-mann-4-enuronosyl groups attheirnon-reducing ends. -D-GalNAc-1,4-β Architecture Architecture Architecture Architecture PL22-PL22 CAZ CAZ CAZ CAZ PL22 PL10 PL15 PL13 PL21 PL12 PL12 PL12 PL8 PL9 PL9 PL2 PL3 PL1 PL1 PL1 PL1 y y y y -D-GlcA bond, ultimately breaking thepolysaccharide 4.2.2.8 and 4.2.2.6 4.2.2.6 4.2.2.8 4.2.2.8 4.2.2.8 4.2.2.9 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.7 4.2.2.7 4.2.2.1 4.2.2.- EC EC EC EC -D-gluc-4-enuronosyl)-D-galacturonate. Optimum Optimum Optimum Optimum Optimum Optimum 9,5-10,5 7,0-10,4 7,0-8,0 7,0-9,0 6,0-7,5 6,7-7,3 10,5 6,0 8,0 pH pH 8,5 8,5 8,5 8,5 6,5 pH pH 7,0 7,0 7,2 7,4 Temperature (ºC) Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum Optimum 40-45 25-37 30 30 60 62 55 55 35 37 32 37 37 37 37 37 37 37 mg/ mg/ mg/ mg/ 0,25 vial vial vial vial 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 -D- 47 cazymes 48 NZYTech 2021 D-galact-4-enuronosyl groups attheirnon-reducing ends. group, to give oligosaccharides with4-deoxy-α Poly- Poly Pectin lyases promote theeliminative cleavage of1,4-α Pectin lyases Pectate lyases ( Catalogue Catalogue Catalogue Number Number Number CZ0408 CZ0460 CZ0886 CZ0846 CZ0309 CZ0304 CZ0885 CZ0398 CZ0836 CZ0558 CZ0530 CZ0834 CZ0303 CZ0528 CZ0539 CZ0697 CZ0728 CZ0755 CZ1090 CZ0513 CZ0417 CZ1077 CZ1091 α -guluronate lyases are enzymes thatparticipate intheeliminative cleavage ofpolysaccharides containing aterminal α -α -guluronate lyases Poly Poly α -guluronate lyase 7A Exo-pectate lyase 9A Exo-pectate lyase 2B α-guluronate lyase 7A Pectate lyase 10A Pectate lyase 10A Pectate lyase 9A Pectate lyase 2A Pectate lyase 2A Pectate lyase 2A Pectate lyase 1A Pectate lyase 1A Pectate lyase 1A Pectate lyase 1A Pectate lyase 1D Pectate lyase 1B Pectate lyase 1A Pectate lyase 1B Pectate lyase 1B Pectate lyase 1B Pectate lyase 1C Pectin lyase 1A Pectin lyase 1A Enzyme Enzyme Enzyme cont Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Pectobacterium carotovorum -L-erythro-hex- 4-enuronosyl groups attheirnon-reducing ends. .) Xanthomonas campestris Pseudomonas marginalis Clostridium cellulovorans Zobellia galactanivorans Zobellia galactanivorans Rhodopirellula baltica Rhodopirellula baltica Rhodopirellula baltica Yersinia enterocolitica Yersinia enterocolitica Erwinia chrysanthemiErwinia Erwinia chrysanthemiErwinia Erwinia chrysanthemiErwinia Bacillus licheniformis Bacillus halodurans Source Organism Source Organism Source Organism Dickeya dadantii Bacillus subtilis Klebsiella sp. -D-galacturonan methyl ester to give oligosaccharides with4-deoxy-6-O-methyl-α Architecture Architecture Architecture CBM32-PL7 CAZ CAZ CAZ PL10 PL10 PL9 PL9 PL2 PL2 PL2 PL2 PL7 PL1 PL1 PL1 PL1 PL1 PL1 PL1 PL1 PL1 PL1 PL1 PL1 PL1 y y y 4.2.2.10 4.2.2.10 4.2.2.11 4.2.2.11 4.2.2.9 4.2.2.9 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 4.2.2.2 EC EC EC Optimum Optimum Optimum Optimum Optimum 9,0-10,0 8,0-8,8 7,0-9,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 11,0 8,0 9,0 9,0 9,0 9,0 6,6 8,6 8,6 8,5 8,5 pH pH pH 7,0 7,5 Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 30-50 30-37 60 50 50 50 30 30 30 55 55 37 37 37 37 37 37 37 37 37 37 37 37 -L-guluronate mg/ mg/ mg/ 0,25 0,25 0,25 0,25 0,25 vial vial vial 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 - residues inXanthan. Enzymes thatcatalyse theβ residues inUlvan. Enzymes thatcatalyze theβ and 4-deoxy-4,5- unsaturated D-galactopyranosyluronic acidatthenon-reducing end. nosyluronic acidbondsofrhamnogalacturonan Idomainsinramified hairy regions ofpectin leaving L-rhamnopyranose atthe reducing end Rhamnogalacturonan lyases are endo-enzymes thatparticipate intheeliminative cleavage ofL-α Rhamnogalacturonan lyases Xanthan lyases Ulvan lyases Catalogue Catalogue Catalogue Number Number Number CZ0040 CZ0693 CZ0067 CZ0704 CZ0740 CZ0744 CZ1080 CZ0527 CZ0186 CZ0881 CZ0725 CZ1069 CZ1083 CZ1070 CZ1074 CZ1072 CZ1073 Rhamnogalacturonan exolyase 26A Rhamnogalacturonan endolyase 11A Rhamnogalacturonan endolyase 9A Rhamnogalacturonan endolyase 9B L-Rhamnose-1,4-α-D-glucuronate Rhamnogalacturonan Lyase 11A Rhamnogalacturonan Lyase 11A Rhamnogalacturonan lyase 4A Xanthan lyase 8A Ulvan lyase 24A Ulvan lyase 25A Ulvan lyase 25A Ulvan lyase 25A Ulvan lyase 25B Ulvan lyase 25A Ulvan lyase 25A Ulvan lyase 25A lyase 27A Enzyme Enzyme Enzyme -eliminative cleavage oftheglycosidic bondbetween thesulfated rhamnose and theglucuronic oriduronic acid -eliminative cleavage oftheglycosidic bondbetween thesulfated rhamnose andtheglucuronic oriduronic acid Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Bacteroides cellulosilyticus Paenibacillus alginolyticus Clostridium thermocellum Catenovulum agarivorans Catenovulum agarivorans Algibacter pectinivorans Nonlabens ulvanivorans Pseudoalteromonas sp. Pseudoalteromonas sp. Cellvibrio japonicus Source Organism Source Organism Source Organism Dickeya dadantii Arenitalea lutea Vibrio celticus Architecture Architecture Architecture CBM66-PL26 CAZ CAZ CAZ PL24 PL25 PL25 PL25 PL25 PL25 PL25 PL25 PL27 PL11 PL11 PL11 PL8 PL9 PL9 PL4 y y y 4.2.2.24 4.2.2.23 4.2.2.23 4.2.2.23 4.2.2.12 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- 4.2.2.- EC EC EC - rhamnopyranosyl-1,4-α Optimum Optimum Optimum Optimum Optimum 8,0-10,0 9,0-10,0 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 6,5-7,5 8,0 6,0 8,0 6,0 9,0 pH pH pH 7,0 Temperature (ºC) Temperature (ºC) Temperature (ºC) Optimum Optimum Optimum 45-55 50 65 45 37 37 37 37 37 37 37 37 37 37 37 37 37 -D-galactopyra- mg/ mg/ mg/ 0,25 0,25 0,25 0,25 0,25 0,25 vial vial vial 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 49 cazymes 50 NZYTech 2021 auxiliar activities Lytic pol LACCASE S y saccharide monoox y genase s * Structure ofCellulase 5Bfrom Bacillus halodurans (PDB4V2X), #CZ0476, pg17 considerable attention owing to theirpotential use inbiomassconversion, notably intheproduction ofbiofuels. such ascellulose andchitin. Membersofthepreviously described families CBM33andfamily GH61are infact LPMOs. These enzymes are attracting Lytic polysaccharide monooxygenases (LPMOs) are a recently discovered class of enzymes capable of oxidizing recalcitrant polysaccharides, L laccases play arole intheformation oflignin by promoting theoxidative coupling ofmonolignols, afamily ofnaturally occurring phenols. molecules, performing aone-electron oxidation of withthe four-electron reduction of molecular oxygen to water. It is proposed that Laccases are copper-containing oxidase enzymes that are found in many plants, fungi and microorganisms. Laccases act on phenols and similar Laccases Catalogue Catalogue Number Number CZ0300 CZ0959 ytic polysaccharide monooxygenases CZ0463 CZ0274 CZ0120 CZ0541 CZ0162 CZ0512 Lytic cellulose monooxygenase 10A Lytic cellulose monooxygenase 10A Lytic cellulose monooxygenase 9B Lytic chitinmonooxygenase 10A Lytic chitinmonooxygenase 10A Laccase 1A Laccase A Laccase A Enzyme Enzyme Geotrichum candidum uncultured bacterium Enterococcus faecalis Bacillus licheniformis Thermobifida fusca Source Organism Source Organism Lactococcus lactis Bacillus subtilis Escherichia coli Architecture Architecture AA NC AA NC CAZ CAZ AA10 AA10 AA10 AA10 AA9 AA1 y y 1.10.3.2 1.10.3.- 1.3.3.5 1.-.-.- 1.-.-.- 1.-.-.- 1.-.-.- 1.-.-.- EC EC Optimum Optimum Optimum 6,0-7,0 8,0 6,0 5,0 3,0 6,5 3,8 pH pH 7,5 Temperature (ºC) Temperature (ºC) Optimum Optimum 50-60 20-60 40 50 45 55 25 37 mg/ mg/ 0,25 0,25 vial vial 0,5 1 1 1 1 1 51 cazymes 52 NZYTech 2021 carbohydrate-binding modules zz-CBM GFP-CBM CBms * Structure ofCarbohydrate BindingModule 42A from Clostridium thermocellum (PDB3KMV), #CZ0047, pg 53 wall hydrolysis. CBMsare classified into numerous families, based onaminoacid sequence similarity inthe CAZy database (www.cazy.org). and different hemicelluloses andpectin. CBMsare the most common non-catalytic modules associated withenzymes active inplant cell- proteins. CBMswere previously known ascellulose-binding domains. CBMscan recognize bothcrystalline andamorphouscellulose forms drolases. The majority ofthese domainshave carbohydrate-binding activity. Someofthese domainsare found oncellulosomal scaffoldin Carbohydrate-binding modules (CBMs) are protein domains found in carbohydrate-active enzymes (CAZymes), for example glycoside hy CBMs Catalogue Number CZ0050 CZ0048 CZ0046 CZ0049 CZ0044 CZ0280 CZ0045 CZ0340 CZ0042 CZ0284 CZ0043 CZ0348 CZ0035 CZ0344 CZ0285 CZ0047 CZ0282 CZ0339 CZ0338 CZ0283 CZ0336 CZ0345 CZ0343 CZ0243 CZ0287 CZ0347 CZ0041 CZ0337 CZ0341 Carbohydrate BindingModule 60A Carbohydrate BindingModule 44A Carbohydrate BindingModule 30A Carbohydrate BindingModule 62A Carbohydrate BindingModule 42D Carbohydrate BindingModule 42A Carbohydrate BindingModule 42B Carbohydrate Binding Module 35A Carbohydrate BindingModule 32A Carbohydrate BindingModule 35B Carbohydrate Binding Module 22A Carbohydrate BindingModule 42C Carbohydrate BindingModule 22B Carbohydrate BindingModule 35C Carbohydrate BindingModule 15A Carbohydrate BindingModule 13A Carbohydrate BindingModule 13C Carbohydrate Binding Module 11A Carbohydrate BindingModule 6D Carbohydrate Binding Module 6A Carbohydrate Binding Module 6A Carbohydrate BindingModule 4A Carbohydrate BindingModule 6B Carbohydrate BindingModule 3D Carbohydrate BindingModule 6E Carbohydrate BindingModule 6C Carbohydrate Binding Module 3A Carbohydrate BindingModule 3E Carbohydrate BindingModule 77 CBM name Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Uncultured bacterium Cellvibrio japonicus Source Organism Celvibrio mixtus Architecture CBM60 CBM44 CBM30 CBM62 CBM42 CBM42 CBM42 CBM42 CBM35 CBM35 CBM35 CBM32 CBM22 CBM22 CBM77 CBM15 CBM13 CBM13 CBM11 CAZ CBM6 CBM6 CBM6 CBM6 CBM6 CBM6 CBM4 CBM3 CBM3 CBM3 y 1,3-1,4-β-glucans andsoluble undecorated (cellu- 1,3-1,4-β-glucans andsoluble undecorated (cellu- decorated andundecorated 1,4-β-xylans. CBM22 specifically recognizes thearabinose side-chains arabinogalactans andweakly to bothdecorated specifically recognizes the galactose side-chains specifically recognizes the galactose side-chains lose) anddecorated (xyloglucan) 1,4-β-glucans lose) anddecorated (xyloglucan) 1,4-β-glucans decorated andundecorated 1,4-β-xylans and decorated andundecorated 1,4-β-xylans and nonreducing endsofmannooligosaccharides 1,4-β-xylan, xyloglucan andgalactomannan 1,3-1,4-β-glucans, 1,3-β-glucans andsoluble 1,3-1,4-β-glucans andsoluble 1,4-β-glucans arabinoxylans andarabinogalactans arabinoxylans and arabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans of xyloglucan andgalactomannan Δ-4,5-anhydro-galacturonic acid also bindsto 1,3-1,4- β-glucans and undecorated 1,4-β-xylans crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose Carbohydrate Ligands of galactomannan arabinogalactans arabinogalactans galactomannans of arabinoxylan 1,4-β-glucans 1,4-β-xylans xyloglucan pectins pectins xylans mg/vial 0,5 0,5 0,5 0,5 1,3 3 2 3 2 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 - 53 cazymes 54 NZYTech 2021 CBMs (CONT.) Catalogue Number CZ0448 CZ0360 CZ0380 CZ0368 CZ0465 CZ0366 CZ0350 CZ0364 CZ0369 CZ0349 CZ0358 CZ0365 CZ0359 CZ0362 CZ0363 CZ0355 CZ0370 CZ0352 CZ0353 CZ0379 CZ0378 CZ0376 CZ0374 CZ0357 CZ0375 CZ0372 CZ0373 CZ0377 CZ0361 CZ0351 CZ0371 Carbohydrate Binding Module 50A Carbohydrate BindingModule 65A Carbohydrate BindingModule 62A Carbohydrate Binding Module 50C Carbohydrate Binding Module 25A Carbohydrate BindingModule 35A Carbohydrate BindingModule 22H Carbohydrate BindingModule 22A Carbohydrate BindingModule 22D Carbohydrate BindingModule 22A Carbohydrate BindingModule 35B Carbohydrate BindingModule 22K Carbohydrate BindingModule 22G Carbohydrate BindingModule 22B Carbohydrate Binding Module 22C Carbohydrate BindingModule 22C Carbohydrate BindingModule 22E Carbohydrate BindingModule 22F Carbohydrate BindingModule 13A Carbohydrate BindingModule 13B Carbohydrate BindingModule 22J Carbohydrate BindingModule 22I Carbohydrate BindingModule 6A Carbohydrate BindingModule 9A Carbohydrate Binding Module 6G Carbohydrate BindingModule 4D Carbohydrate Binding Module 4B Carbohydrate Binding Module 4B Carbohydrate BindingModule 4B Carbohydrate Binding Module 6F Carbohydrate BindingModule 4C CBM name Eubacterium cellulosolvens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Thermotoga maritima Source Organism Architecture CBM65_1 CBM9_1- CBM9_2 CBM50 CBM50 CBM62 CBM25 CBM35 CBM35 CBM22 CBM22 CBM22 CBM22 CBM22 CBM22 CBM22 CBM22 CBM22 CBM22 CBM22 CBM22 CBM22 CBM13 CBM13 CAZ CBM6 CBM6 CBM4 CBM4 CBM6 CBM4 CBM4 CBM4 y both decorated andundecorated 1,4-β-xylans and 1,4-1,6-α-glucans, suchaspullulan, andα-manno- preference for decorated 1,4-β 1,4-β linear 1,3-β-glucans withoccasional decorations linear 1,3-β-glucans withoccasional decorations linear 1,3-β-glucans withoccasional decorations undecorated linear 1,3-β-glucans ordecorated arabinoxylan andweakly to arabinogalactan -glucans and1,3-1,4-β arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans andarabinogalactans arabinoxylans and arabinogalactans non-reducing endsofcellulose with 1,6-β-glucose side-chains of 1,6-β-glucose side-chains of 1,6-β-glucose side-chains of 1,6-β-glucose side-chains Carbohydrate Ligands arabinan anpectins se polysaccharides arabinogalactans galactomannans galactomannans 1,3-1,4-β-glucans 1,3-1,4-β-glucans 1,3-1,4-β-glucans arabinoxylan pectins -glucans butdisplays a -glucans (xyloglucan) mg/vial 0,25 0,25 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 CBMs (CONT.) Catalogue Number CZ0890 CZ0500 CZ0889 CZ0468 CZ0466 CZ0506 CZ0484 CZ0504 CZ0469 CZ0859 CZ0505 CZ0862 CZ0983 CZ0482 CZ0483 CZ0878 CZ0470 CZ0876 CZ0507 CZ0467 CZ0874 CZ0875 CZ0873 CZ0475 CZ0474 CZ0473 CZ0472 CZ0877 CZ0752 CZ0501 CZ0471 CZ1014 Carbohydrate BindingModule 2b-2 Carbohydrate Binding Module 80A Carbohydrate BindingModule 66A Carbohydrate BindingModule 64A Carbohydrate Binding Module 65A Carbohydrate BindingModule 56A Carbohydrate BindingModule 56A Carbohydrate BindingModule 2b-1 Carbohydrate BindingModule 28A Carbohydrate BindingModule 26A Carbohydrate BindingModule 63A Carbohydrate BindingModule 56B Carbohydrate Binding Module 78A Carbohydrate Binding Module 79A Carbohydrate BindingModule 76A Carbohydrate BindingModule 47A Carbohydrate BindingModule 75A Carbohydrate BindingModule 57A Carbohydrate BindingModule 27A Carbohydrate BindingModule 16A Carbohydrate BindingModule 61A Carbohydrate BindingModule 41A Carbohydrate BindingModule 51A Carbohydrate BindingModule 21A Carbohydrate BindingModule 17A Carbohydrate Binding Module 6A Carbohydrate Binding Module 4A Carbohydrate BindingModule 5A Carbohydrate BindingModule 2D Carbohydrate Binding Module 2b Carbohydrate BindingModule 2F Carbohydrate Binding Module CBM name Rf999 Bacteroides thetaiotaomicron Caldanaerobius polysaccha- Eubacterium cellulosolvens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Ruminococcus flavefaciens Clostridium cellulovorans Spirochaeta thermophila Clostridium perfringens Thermotoga maritima Thermotoga maritima Thermotoga maritima Streptococcus mitis Bacillus halodurans Pyrococcus furiosus Bacillus halodurans Thermobifida fusca Thermobifida fusca Source Organism Cellulomonas fimi Cellulomonas fimi Cellulomonas fimi Cellulomonas fimi Bacillus circulans Paenibacillus sp. Paenibacillus sp. Clostridium josui Bacillus subtilis Bacillus subtilis Homo sapiens rolyticus Architecture CBM Rf999 CBM65_2 CBM2b-2 CBM2b-1 CBM80 CBM66 CBM64 CBM56 CBM56 CBM56 CBM28 CBM26 CBM63 CBM2b CBM78 CBM79 CBM76 CBM47 CBM75 CBM57 CBM27 CBM16 CBM61 CBM41 CBM51 CBM21 CBM17 CAZ CBM6 CBM4 CBM5 CBM2 CBM2 y non-crystalline cellulose andsoluble 1,4-β-glucans preference for decorated 1,4-β crystalline forms ofcellulose. The protein isa bac 1,3-1,4-β-glucans, soluble 1,4-β-glucans, xyloglu- 1,3-1,4-β-glucans, soluble 1,4-β-glucans, xyloglu- terial expansin thatpromotes root colonization 1,4-β 1,3-β-glucans suchaslaminarin andcurdlan 1,3-β-glucans suchaslaminarin andcurdlan 1,3-β-glucans suchaslaminarin andcurdlan 1,3-1,4-β-glucans, soluble 1,4-β-glucans and α-glucooligosaccharides, particularlythose galactose residues inoligosaccharides -glucans and1,3-1,4-β fucose containing oligosaccharides glucose-, mannose-, andglucose/ mannose-configured substrates crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose cellooligosaccharide ligands can andgalactomannans can andgalactomannans xylans andarabinoxylans containing α-1,6 linkages Carbohydrate Ligands linear arabinans 1,4-β-galactans 1,4-β-mannans 1,4-α-glucans 1,4-α-glucans 1,4-β-glucans 1,3-β-glucans 1,3-β-glucans 1,4-β-xylans 1,4-β-xylans 1,4-β-xylans xyloglucan xyloglucan fructans chitin -glucans butdisplays a -glucans (xyloglucan) - mg/vial 0,25 0,25 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 55 cazymes 56 NZYTech 2021 that exhibits brightgreen fluorescence when exposed to light intheblue to ultraviolet range. GFPfused CBMs are excellent tools for cell signalling studies. NZYTech has developed a series of CBMs fused with the green fluorescent protein (GFP). GFP is a protein composed of 238 residues (26.9 kDa) GFP-CBM CBMs (CONT.) Catalogue Catalogue Number Number CZ0080 CZ0069 CZ0058 CZ0059 CZ0082 CZ0083 CZ0070 CZ0079 CZ0078 CZ0076 CZ0074 CZ0075 CZ0057 CZ0073 CZ0072 CZ0077 CZ0081 CZ1048 CZ1020 CZ1026 CZ1029 CZ0071 CZ1022 CZ1016 CZ1018 CZ1019 CZ1015 CZ1021 CZ1017 Carbohydrate Binding Module 66A Carbohydrate BindingModule 48A Carbohydrate Binding Module 44A Carbohydrate Binding Module 20A Carbohydrate Binding Module 28A Carbohydrate Binding Module 34A Carbohydrate Binding Module 32A Carbohydrate Binding Module 32A Carbohydrate BindingModule 22A Carbohydrate Binding Module 67A Carbohydrate Binding Module 32B Carbohydrate BindingModule 10A Carbohydrate BindingModule 10A Carbohydrate BindingModule 9A Carbohydrate BindingModule 9A Carbohydrate BindingModule 3A Carbohydrate BindingModule 2A Carbohydrate BindingModule 3A Carbohydrate BindingModule 3A Carbohydrate BindingModule 3A Carbohydrate BindingModule 3A Carbohydrate BindingModule 2A Carbohydrate BindingModule 3A Carbohydrate BindingModule 2A Carbohydrate BindingModule 3A Carbohydrate BindingModule 3A Carbohydrate BindingModule 3B Carbohydrate BindingModule 2B Carbohydrate BindingModule 2C CBM name CBM name Bacteroides thetaiotaomicron Ruminococcus champanel Geobacillus stearothermo- Clostridium cellulolyticum Caldicellulosiruptor bescii Zobellia galactanivorans Clostridium perfringens Clostridium perfringens Paenibacillus barcinonensis Acidothermus cellulolyticus Streptococcus mutans Bacteroides cellulosolvens Clostridium thermocellum Caldicellulosiruptor bescii Clostridium stercorarium Acetivibrio cellulolyticus Source Organism Teredinibacter turnerae Clostridium clariflavum Thermotoga maritima Thermotoga maritima Ampullaria crossean Pyrococcus furiosus Thermobifida fusca Thermobifida fusca Thermobifida fusca Cellvibrio japonicus Source Organism Rhizobium sp. Bacillus sp. Bacillus subtilis philus lensis - CBM32-CBM32 Architecture PKD-CBM44 CBM66 CBM48 CBM20 CBM28 CBM34 CBM32 CBM32 CBM22 CBM67 CAZ Architecture GFP-CBM9_1- GFP-CBM10 GFP-CBM10 GFP-CBM9 GFP-CBM3 GFP-CBM3 GFP-CBM3 GFP-CBM2 GFP-CBM3 GFP-CBM3 GFP-CBM3 GFP-CBM2 GFP-CBM2 GFP-CBM2 GFP-CBM2 GFP-CBM3 GFP-CBM3 GFP-CBM3 CBM9_2 y CAZ y terminal fructoside residue offructans galactose residues inoligosaccharides cans, 1,4-β cans, 1,4-β LacNAc (β-D-galactosyl-1,4-β-D-N- the reducing endsof1,3-1,4-β the reducing endsof1,3-1,4-β crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose cellooligosaccharide ligands crystalline forms ofchitin crystalline forms ofchitin Carbohydrate Ligands cellulose and xyloglucan Carbohydrate Ligands acetylglucosamine) -glucans1,4- and -glucans and1,4- cyclodextrins L-rhamnose glycogen alginate xylans β-xylans β-xylans -glu -glu - - mg/vial 0,5 0,5 0,5 0,5 0,5 5 1 1 1 1 1 1 1 1 1 1 1 1 mg/vial 0,5 0,5 1 1 1 1 1 1 1 1 1 GFP-CBM (CONT.) Catalogue Number CZ0090 CZ0088 CZ0086 CZ0096 CZ0084 CZ0089 CZ0094 CZ0085 CZ0095 CZ0496 CZ0093 CZ0092 CZ0286 CZ0502 CZ0503 CZ0346 CZ0087 CZ0354 CZ0497 CZ0342 CZ0335 CZ0091 CZ1038 CZ1036 CZ0142 CZ1034 CZ1035 CZ1032 CZ1033 CZ1037 CZ0177 CZ1031 Carbohydrate BindingModule 2b-2 Carbohydrate BindingModule 66A Carbohydrate BindingModule 48A Carbohydrate BindingModule 48A Carbohydrate Binding Module 64A Carbohydrate BindingModule 44A Carbohydrate BindingModule 20A Carbohydrate BindingModule 2b-1 Carbohydrate BindingModule 30B Carbohydrate BindingModule 62A Carbohydrate Binding Module 63A Carbohydrate BindingModule 34A Carbohydrate BindingModule 32A Carbohydrate BindingModule 32A Carbohydrate BindingModule 22A Carbohydrate BindingModule 27A Carbohydrate BindingModule 61A Carbohydrate BindingModule 11A Carbohydrate BindingModule 6A Carbohydrate BindingModule 6A Carbohydrate BindingModule 4A Carbohydrate BindingModule 2D Carbohydrate Binding Module 2A Carbohydrate Binding Module 3A Carbohydrate BindingModule 3A Carbohydrate BindingModule 3A Carbohydrate Binding Module 2G Carbohydrate BindingModule 3C Carbohydrate Binding Module 2E Carbohydrate Binding Module 2F Carbohydrate Binding Module 1A Carbohydrate Binding Module 1A CBM name Geobacillus stearothermophilus Clostridium acetobutylicum Neocallimastix patriciarum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Clostridium cellulolyticum Clostridium cellulolyticum Spirochaeta thermophila Zobellia galactanivorans Clostridium stercorarium Butyrivibrio fibrisolvens Clostridium perfringens Streptococcus mutans Thermotoga maritima Thermotoga maritima Nostoc punctiforme Bacillus halodurans Thermobifida fusca Thermobifida fusca Thermobifida fusca Thermobifida fusca Trichoderma reesei Source Organism Cellulomonas fimi Cellulomonas fimi Cellulomonas fimi Bacillus subtilis Escherichia coli Bacillus sp. GFP-PKD-CBM44 GFP-CBM2b-2 GFP-CBM2b-1 Architecture GFP-CBM66 GFP-CBM48 GFP-CBM48 GFP-CBM64 GFP-CBM30 GFP-CBM20 GFP-CBM62 GFP-CBM63 GFP-CBM34 GFP-CBM32 GFP-CBM32 GFP-CBM22 GFP-CBM27 GFP-CBM61 GFP-CBM11 GFP-CBM6 GFP-CBM6 GFP-CBM4 GFP-CBM2 GFP-CBM3 GFP-CBM2 GFP-CBM3 GFP-CBM3 GFP-CBM3 GFP-CBM2 GFP-CBM2 GFP-CBM2 GFP-CBM1 GFP-CBM1 CAZ y 1,3-1,4-β-glucans andsoluble unde- 1,3-1,4-β-glucans and1,4-β-glucans arabinoxylans andarabinogalactans LacNAc (β-D-galactosyl-1,4-β-D-N corated (cellulose) anddecorated sin thatpromotes root colonization xyloglucan andgalactomannan; galactose side-chainsofthese the protein isa bacterial expan - crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose crystalline forms ofcellulose it specifically recognizes the 1,4-β-xylans; italso bindsto decorated andundecorated crystalline forms ofcellulose; terminal fructoside residue (xyloglucan) 1,4-β-glucans cellulose andxyloglucan Carbohydrate Ligands -acetylglucosamine) 1,3-1,4-β-glucans polysaccharides 1,4-β-galactans 1,4-β-mannans 1,3-β-glucans 1,3-β-glucans cyclodextrins 1,4-β-xylans 1,4-β-xylans of fructans glycogen glycogen alginate pullulan mg/vial 0,25 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 0,5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 57 cazymes 58 NZYTech 2021 coccus aureus. This ZZ domain protein derivative is particularly recommended to mediate the anchorage of antibodies to cellulosic supports. ZZ-CBMs are modular proteins where different CBMsare fused to theimmunoglobulin G(IgG) binding ZZ domainofprotein Afrom Staphylo- zz-CBM GFP-CBM (CONT.) Catalogue Catalogue Number Number CZ0498 CZ0499 CZ1040 CZ1046 CZ1045 CZ1039 CZ1043 CZ1047 CZ1041 Carbohydrate Binding Module 48A Carbohydrate BindingModule 64A Carbohydrate Binding Module 28A Carbohydrate Binding Module 34A Carbohydrate Binding Module 22A Carbohydrate Binding Module 67A Carbohydrate Binding Module 32B Carbohydrate Binding Module 57A Carbohydrate BindingModule 3A CBM name CBM name Ruminococcus champanellensis Bacteroides thetaiotaomicron Bacteroides thetaiotaomicron Clostridium thermocellum Caldicellulosiruptor bescii Spirochaeta thermophila Clostridium perfringens Source Organism Source Organism Escherichia coli Rhizobium sp. Architecture Architecture GFP-CBM32- GFP-CBM48 GFP-CBM28 GFP-CBM34 GFP-CBM67 GFP-CBM22 GFP-CBM57 ZZ-CBM64 ZZ-CBM3 CBM32 CAZ CAZ y y crystalline forms ofcellulose crystalline forms ofcellulose cellooligosaccharide ligands xylans andarabinoxylans Carbohydrate Ligands Carbohydrate Ligands galactose residues in oligosaccharides L-rhamnose glycogen xylans starch mg/vial mg/vial 0,5 1 1 1 1 1 1 1 1 Xylose isomerase Cellobiose dhy droge nase Mini-CELLULOSOME S * Structure ofClostridium thermocellum Mini-Cellulosome (PDB EMD-1823), #CZ0065, pg60

mini-cellulosomes & other enzymes 59 cazymes 60 NZYTech 2021 partners to produce tailor madenovel mini-cellulosomes combinations. zyme proximity. Inthiscatalogue, NZYTech hasmadeavailable three novel mini-cellulosome combinations butcan cooperate withdifferent assembly and protein functionto produce highly efficientmulti-enzyme complexes for all biological processes thatmightbenefit fromen- manner in the degradation of cellulosic substrates. At NZYTech, we can integrate our current knowledge on the mechanisms of cellulosome polysaccharides. Previous research ondesignercellulosomes hasshown thatcellulosomal cellulases acttogether inanenhanced synergistic It iswell established thatcellulosomes are elaborate “Lego”-like structures thatperform theefficient hydrolysis of plant cell wall recalcitrant Mini-CELLULOSOMES ases, specifically those intramolecular interconverting aldoses and ketoses. Xylose are enzymes thatcatalyze theinterconversion ofD-xylose andD-xylulose. These enzymes belong to thefamily ofisomer X quinones, phenoxyradicals, Fe cellodextrins efficiently totheir corresponding lactones byaping-pong mechanismusingawidespectrumof electron acceptors including Cellobiose dehydrogenases (CDH) are extracellular enzymes produced by various lignocellulose-degrading microbes. They oxidize soluble Cellobiose dehydrogenase Catalogue Catalogue Catalogue Number Number Number CZ0960 CZ0068 CZ0066 CZ0065 CZ0887 ylose isomerase Cellulase 8A, Mini-Cellulosome Cellulase 8A, Mini-Cellulosome Lichenase 26A, Mini-Cellulo- Cellobiose dehydrogenase Xylose isomerase A Enzyme Enzyme Enzyme some 3+ , Cu , 2+ andtriiodideion. Clostridium thermocellum Clostridium thermocellum Clostridium thermocellum Podospora anserina Source Organism Source Organism Source Organism thetaiotaomicron Bacteroides 3×GH8 +Coh-CBM3-Coh-Coh 3×GH16 +Coh-Coh-Coh 3×GH8 +Coh-Coh-Coh Architecture Architecture Architecture CAZ CAZ CAZ CDH N/A y y y 1.1.99.18 3.2.1.73 5.3.1.5 3.2.1.4 3.2.1.4 EC EC EC Optimum Optimum Optimum Optimum 5.0-7.5 5.0-7.5 5.5-7.0 6.5-7.5 5,0 pH pH pH Temperature (ºC) Temperature (ºC) Temperature (°C) Optimum Optimum Optimum 60 60 30 65 37 mg/ mg/ mg/ 0,25 vial vial vial 0,5 0,5 1 1 - NZYTech can shipatdifferent temperatures products to bestored at4°Cor -20 °C, inthe cases where thisdoesnot affect All products are transported inappropriate conditions to maintain intact all theproperties inherent to theirexpected output. Product ShippingandDelivery Custom fees related to theclearance ofgoods uponproduct arrival to thedestiny country are ofCUSTOM ER responsibility. 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Banking expenses should befully supported by theclient. Inspecific cases, theproduction will only take place after bank vali- For international sales, advanced payment isrequired andorders are only processed uponbank validation ofmoney transfer. Payment Terms prices have beennominally changed. Price increases andreductions, ifany, will beautomatically applied to your invoice. and NZYTech website exclude VAT, whichmightbe applied whentheinvoice isissued. Shipmentwill bemadepromptly even if or fax. If you order via our website, we will guarantee theprice shown at the website at that time. Prices shown incatalogue without aPurchase Order. NZYTech will notaccept orders by phone. Orders are only accepted viawebsite (online store), e-mail When placing your order, please refer thequote orpro-forma invoice number. NZYTech will notinitiate theinvoicing process Orders which quotations are valid for 30days. pre-payment onany order, inNZYTech sole discretion. We guarantee written quotations for 90days, except for promotions, for Prices are shown inEuro (€). Prices may besubjected to change withoutpriornotice. NZYTech reserves therightto require Pricing complaint. ingly, i.e., include invoice numberorPurchase Order number, batch number, problem description andevidence to sustain the (CoA). NZYTech will require theCUSTOMER to provide all thenecessary information inorder to process thecomplaint accord- Complaints regarding product quality will only beaccepted aslong astheproduct inquestionhasavalid certificate ofanalysis Complaints as itmay choose to replace itfree ofcharge orrefund thepurchase price. uct itself has a valid Certificate of Analysis (CoA). If a product fails to conform to its specifications, NZYTech should be informed NZYTech warrants thatitsproducts conform to thespecifications intheaccompanying technical brochure as long astheprod - Warranty Agricultural orPesticide Products, Cosmetics, Drugs, Food Additives orHousehold Chemicals. Product Brochures andMaterial Safety Data Sheets(MSDS) available for download. NZYTech products should NOT beused as information abouttheproduct before starting thework. Please visitourwebsite to findoutmore details abouttheproducts, NZYTech products, services, kitsandreagents are for laboratory research andinvitro use only. Customers mustread all relevant Use ofProducts TERMS &CONDITIONS 61 cazymes 62 NZYTech 2021 We reserve therightto discontinue any product withoutpriornotice. Discontinuation ofProducts mistake, butmay besubjected to aprocessing fee of30%to cover costs. will take aquickandappropriate actionto correct theproblem. Returns may beaccepted for items thathave beenordered by result of a mistake from our Logistics team, please contact our Customer Service through e-mail at [email protected], and we tomer Service will provide assistance, shippinginstructionsandauthorizations. Ifanitem isshippedto you incorrectly, asthe Quality Department through e-mail at [email protected]. If the damage requires you to return the goods to NZYTech, our Cus- case ofany damage occur, please retain thedamaged goods, packing material andshippingdocuments please notifyour that theproducts were delivered withoutincidentsandnolonger accepts any complaint regarding packaging ordelivery. In will only have 10working days to notifyNZYTech ifany damage ordiscrepancy occurs. After thistime, NZYTech will assume must inspectall packages immediately uponreceipt andstore thegoods atthedesignated storage temperature. CUSTOMER NZYTech Customer Service is available to assist you in every way possible if a problem arises with your order. The CUSTOMER Return Policy ments mustbestated uponplacement oftheorder, asthese documentswill beprovided atanadditionalcost. In case acustomer requires any certification to be sent withthe goods, i.e., Certificate ofOriginor ATR forms, these require- has itsown deadline. For most countries, thedelivery occurs withinfive working days, except in case ofstock rupture. vices, including gene synthesis, DNAsequencing andoligonucleotides, are only produced ondemandand, therefore, each order products, Molecular Diagnostics kits, Molecular Services andbulkorders can take longer to produce andshipthegoods. All Ser we handle the shipment or EXW - Ex-Works, whenusing a courier customer’s account. In specific cases, like CAZymes, Analytical product quality. Regarding Incoterms, NZYTech works withtheDelivery Terms asDAP –Delivered atPlace ofdestination, where - 63 cazymes Catalogues by NZYTech

Customer Service Team +351 21 364 35 14

www.nzytech.com [email protected]