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2010 Physical Biosciences Research Meeting 2010 Physical Biosciences Research Meeting Sheraton Inner Harbor Hotel Baltimore, MD October 17-20, 2010 Office of Basic Energy Sciences Chemical Sciences, Geosciences & Biosciences Division 2010 Physical Biosciences Research Meeting Program and Abstracts Sheraton Inner Harbor Hotel Baltimore, MD October 17-20, 2010 Chemical Sciences, Geosciences, and Biosciences Division Office of Basic Energy Sciences Office of Science U.S. Department of Energy i Cover art is taken from the public domain and can be found at: http://commons.wikimedia.org/wiki/File:Blue_crab_on_market_in_Piraeus_-_Callinectes_sapidus_Rathbun_20020819- 317.jpg This document was produced under contract number DE-AC05-060R23100 between the U.S. Department of Energy and Oak Ridge Associated Universities. The research grants and contracts described in this document are, unless specifically labeled otherwise, supported by the U.S. DOE Office of Science, Office of Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division. ii Foreword This volume provides a record of the 2nd biennial meeting of the Principal Investigators (PIs) funded by the Physical Biosciences program, and is sponsored by the Chemical Sciences, Geosciences, and Biosciences Division of the Office of Basic Energy Sciences (BES) in the U.S. Department of Energy (DOE). Within DOE-BES there are two programs that fund basic research in energy-relevant biological sciences, Physical Biosciences and Photosynthetic Systems. These two Biosciences programs, along with a strong program in Solar Photochemistry, comprise the current Photo- and Bio- Chemistry Team. This meeting specifically brings together under one roof all of the PIs funded by the Physical Biosciences program, along with Program Managers and staff not only from DOE-BES, but also other offices within DOE, the national labs, and even other federal funding agencies. Of course we also have some distinguished speakers who we hope will stimulate your thinking, and inform you about new tools and resources that will allow you to meet - or exceed - your current research objectives; more on that below. Our objective in holding these research meetings is to provide an environment that (1) encourages free exchange of information on your DOE-funded work; (2) facilitates new collaborations between individual research groups with complementary strengths; (3) allows opportunities for discussions with DOE Program Managers and staff; (4) exposes you to new ideas and methodologies; and (5) supplies information on DOE User Facilities, and how one goes about gaining access to them. In that regard, this year’s agenda features several invited speakers from the DOE national labs. We are delighted to feature Allen Orville from Brookhaven National Laboratory as one of our featured “idea- generators” on the physical science side, and Bryan Linggi and John Cort from the Pacific Northwest National Laboratory (PNNL) who will tell you about the exciting “-omics” capabilities at PNNL’s Environmental Molecular Sciences Lab (EMSL) – and how one goes about accessing them. Ian Carmichael from the Notre Dame Radiation Laboratory has also been invited to provide a brief talk on how he and his colleagues are trying to address the serious problem of radiation damage to protein structures that is caused by x-ray beam lines, and why it is a critically important problem to address. While we extend a warm welcome and our sincere appreciation to our invited speakers, the real star at this year’s meeting is…you. It is your hard work, creativity, productivity, and commitment to world- class science that comes across in your submitted abstracts. Whether you are delivering a talk or presenting a poster, we are sincerely appreciative of your contribution to this meeting. The depth and breadth of the DOE-BES Biosciences portfolio is what makes a meeting like this not only exciting, but also – we hope – a very fun and rewarding one to attend. It has been an extraordinary period of time in DOE’s Office of Science, and we want to thank you for your many contributions to the successful execution of the many calls we have put out over the last two years. Finally, we also wish to thank Diane Marceau from DOE-BES and Connie Lansdon from Oak Ridge Institute for Science and Education (ORISE) for their invaluable help in planning and successfully executing the many logistical tasks associated with putting on this meeting. Robert J. Stack, Program Manager, Physical Biosciences, DOE-BES B. Gail McLean, Program Manager, Photosynthetic Systems, DOE-BES Richard V. Greene, Lead, Photo- and Bio-Chemistry Team, DOE-BES iii iv Agenda Agenda AGENDA 2010 Physical Biosciences Research Meeting Sheraton Inner Harbor Hotel, Baltimore, MD October 17-20, 2010 Sunday, October 17, 2010 3:00 – 6:00 p.m. Registration 5:30 – 6:30 Reception (No Host) 6:30 – 7:30 Dinner at Sheraton Inner Harbor Hotel 7:30 – 8:00 Welcome, Opening Remarks, and DOE Update/News Robert Stack, Program Manager, Physical Biosciences, DOE-BES Eric Rohlfing, Director, Chemical Sciences, Geosciences & Biosciences Division, DOE-BES Monday, October 18, 2010 7:15 – 8:00 a.m. Continental Breakfast Session I: Physical Science Tools for Energy Transduction Studies 8:00 – 8:30 a.m. Welcome and Physical Biosciences Program Update Robert Stack, Program Manager, Physical Biosciences 8:30 – 9:30 More than Simply Atomic Structure: Correlated Single-Crystal Spectroscopy and X-ray Diffraction Allen Orville, Brookhaven National Laboratory 9:30 – 10:00 Break Session II: Hydrogen Metabolism and Electron Flux in Microbial Systems Joe Krzycki, Moderator 10:00 – 10:30 Enzymology of Methanogenesis: Mechanism of Methyl-Coenzyme M Reductase Stephen Ragsdale, University of Michigan 10:30 – 11:00 Electron Bifurcation and Novel Pathways of Electron Flow from Formate in a Model Hydrogenotrophic Methanogen John Leigh, University of Washington 11:00 – 11:30 Genetics and Molecular Biology of Hydrogen Metabolism in Sulfate- Reducing Bacteria Judy Wall, University of Missouri 11:30 – 12:00 Genetic Analysis of Hydrogenotrophic Methanogenesis in Methanosarcina Species William Metcalf, University of Illinois 12:00 – 1:00 Lunch Session III: Plant Growth and Regulation Gloria Coruzzi, Moderator 1:00 – 1:30 Regulation of Actin Filament Ends: The Role of Capping Protein in Stochastic Dynamics and Organelle Behavior Christopher Staiger, Purdue University v 1:30 – 2:00 Cellulose Synthesis and the Control of Growth Anisotropy Tobias Baskin, University of Massachusetts 2:00 – 2:30 Exploring Molecular Mechanisms of Lignin Biosynthesis and Its Regulation Chang-Jun Liu, Brookhaven National Laboratory Session IV: Special Guest Lecture/Life Sciences Research Fellow Bob Stack, Moderator 2:30 – 3:00 Spatial and Temporal Organization of Cyanobacterial Metabolism David Savage, Harvard Medical School 3:00 – 6:00 Free/Discussion Time (Put up your poster too!) 6:00 – 6:30 Reception (No-Host) 6:30 – 7:30 Dinner at Sheraton Inner Harbor Hotel Poster Session I 7:30 – 9:30 Odd Numbered Posters (No-Host) Tuesday, October 19, 2010 7:15 – 8:00 a.m. Continental Breakfast Session V: ‘Omics Tools for Energy Transduction Studies Bob Stack, Moderator 8:00 – 9:00 EMSL: A National Scientific User Facility for State-of-the-Art Molecular and Environmental Research Bryan Linggi & John Cort, EMSL, Pacific Northwest National Laboratory 9:00 – 9:30 Break Session VI: Signal Transduction in Plants Elizabeth Vierling, Moderator 9:30 – 10:00 A Proteomic Study of Brassinosteroid Responses in Plants Zhiyong Wang, Carnegie Institute 10:00 – 10:30 The Role of Auxin in Ambient Temperature Growth Regulation Mark Estelle, University of California, San Diego 10:30 – 11:00 Plant Response to LCO/CO Signals Gary Stacey, University of Missouri 11:00 – 11:30 The Crystal Structure of a Self-Activating Gα Protein Reveals a New Mechanism of Signal Initiation Alan Jones, University of North Carolina Session VII: The Archaeal Proteasome Rick Vierstra, Moderator 11:30 – 12:00 Proteasomes and Post-translational Modification of Haloferax volcanii Proteins Julie Maupin-Furlow, University of Florida 12:00 – 1:00 Lunch vi Session VIII: Pumps, Transporters, and Trafficking in Plants Julian Schroeder, Moderator 1:00 – 1:30 Molecular Mechanism and Biological Function of the Plasma Membrane Proton Pump (H+-ATPase) of Arabidopsis thaliana Michael Sussman, University of Wisconsin Biotechnology Center 1:30 – 2:00 FKBP-Mediated Maturation and Sterol Packing of the Arabidopsis ABCB19 Auxin Transporter are Distinct Processes Angus Murphy, Purdue University 2:00 – 2:30 Trafficking to the Plant Storage Vacuoles in Plants Natasha Raikhel, University of California, Riverside 2:30 – 3:00 Functional Analysis of Plant Sucrose Transporters John Ward, University of Minnesota 3:00 – 7:30 Free/Discussion Time and Dinner on Your Own Poster Session II 7:30 – 9:30 Even Numbered Posters (No-Host) Wednesday, October 20, 2010 7:15 – 8:00 a.m. Continental Breakfast Session IX: Meeting the Challenges of X-ray Studies Bob Stack, Moderator 8:00 – 8:30 Radiation Damage in Macromolecular Crystallography Ian Carmichael, Notre Dame Radiation Laboratory 8:30 – 9:00 Energetics and Structure of the ZIP Metal Transporter Dax Fu, Brookhaven National Laboratory 9:00 – 9:30 Break Session X: Bio-inspired Structural Design Jay Groves, Moderator 9:30 – 10:00 Engineering Functional Scaffolds by Supramolecular Self-Assembly David Lynn, Emory University 10:00 – 10:30 Nanotube-Supported Phospholipid Bilayers: Self-Assembly and Nanoscale Confinement Alex Smirnov, North Carolina
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