Cultivating the Uncultured: Growing the Recalcitrant Cluster-2 Frankia Strains
Gtari M, Ghodhabane-Gtari F, Nouioui I, Gtari A, Hezbri K, Mimouni W, Sbissi I, Ayari A, Yamanaka T, Normand P, Tisa L, Boudabous A. Cultivating the uncultured: growing the recalcitrant cluster-2 Frankia strains. Scientific Reports 2015, 5, 13112. Copyright: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. DOI link to article: http://dx.doi.org/10.1038/srep13112 Date deposited: 01/07/2016 This work is licensed under a Creative Commons Attribution 4.0 International License Newcastle University ePrints - eprint.ncl.ac.uk www.nature.com/scientificreports OPEN Cultivating the uncultured: growing the recalcitrant cluster-2 Frankia strains Received: 25 February 2015 1 1 1 1 1 Accepted: 15 July 2015 Maher Gtari , Faten Ghodhbane-Gtari , Imen Nouioui , Amir Ktari , Karima Hezbri , 1 1 1 2 3 Published: 19 August 2015 Wajdi Mimouni , Imed Sbissi , Amani Ayari , Takashi Yamanaka , Philippe Normand , Louis S Tisa4 & Abdellatif Boudabous1 The repeated failures reported in cultivating some microbial lineages are a major challenge in microbial ecology and probably linked, in the case of Frankia microsymbionts to atypical patterns of auxotrophy. Comparative genomics of the so far uncultured cluster-2 Candidatus Frankia datiscae Dg1, with cultivated Frankiae has revealed genome reduction, but no obvious physiological impairments. A direct physiological assay on nodule tissues from Coriaria myrtifolia infected with a closely-related strain permitted the identification of a requirement for alkaline conditions.
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