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Gibasis Geniculata) by Lucas Mackenzie April 12, 2018

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Gibasis Geniculata) by Lucas Mackenzie April 12, 2018 PLNT-310 Plant Propagation Propagation of Tahitian Bridal Veil (Gibasis geniculata) By Lucas Mackenzie April 12, 2018 My name is Lucas Mackenzie, I’m a U2 student studying Environmental Biology and specializing in Plant Biology at McGill University’s faculty of Agriculture and Environmental Science. This paper summarizes the experiment I performed as part of the PLNT- 310 Plant Propagation course offered by Dr. Danielle Donnelly. The experiment involved propagation Tahitian Bridal Veil (Gibasis geniculata) by stem cuttings while treating them with different potting materials and the addition of auxins. Species used: Tahitian Bridal Veil (Gibasis geniculata) Tahitian Bridal Veil is an ornamental plant grown all around the world. It is an Angiosperm monocot belonging to the family Commelinaceae and order Commelinales. It is a perennial with non- tuberous roots, has a creeping growth habit and can survive on calcareous or non-calcareous soil. This plant has many small white flowers and lanceolate to ovate leaves that are dark green on the adaxial side and purple on the abaxial side (Acevedo-Rodriguez and Strong, 2005). It is native to humid New World tropical environments and can be found in South America, Central America and the Caribbean where it lives as an understory plant. It grows best in shade with high humidity, but it is a hardy plant that can tolerate full sun, shaded environments, being dry or even temperatures as low as -9.4°C (Gibasis geniculata, n.d., Tahitian Bridal Veil, n.d.). Objectives: 1) Evaluate the effects of rooting hormone powder on root and shoot growth of G. geniculata stem cuttings. 2) Evaluate the effects of potting material on root and shoot growth of G. geniculata stem cuttings. Rooting Hormone Powder In this experiment Stim-Root #1 rooting hormone powder was used to test the effects of auxins on G. geniculata stem cuttings. Stim-Root #1 is a commercial rooting hormone stimulant and it contains 0.1% IBA. IBA (Indole-3-butyric acid) is an auxin chemical naturally found in plants and is added to cuttings to promote rooting. Some of the effects of auxins include the inhibition of lateral buds, phototropism, gravitropism, fruit development and adventitious root formation, which is what this experiment tested (Stefancic et al., 2005). Potting Materials This experiment tested the effects of perlite versus Agromix (G6) potting mixtures. Perlite is made from volcanic glasses that has been heated, crushed and expanded. It is used for its high porosity and fast drainage, it is often used to start cuttings (Horticultural grade perlite, 1970). G6 is an all-purpose potting mixture composed mainly of peat with some perlite, coconut husk, limestone, micronutrients and gypsum (Agro Mix G6, n.d.). This potting mixture is used for many different purposes and drains less quickly and is less aerated than perlite but contains many nutrients and retains water. Experiment The experiment involved taking 2 node stem cuttings from the plant. These cuttings were done by cutting off 2 node pieces of the stem with a sterilized razor blade, removing the bottom leaf so that each cutting only had a single leaf and finally placing the cutting in its appropriate potting mixture with its appropriate treatment of rooting hormone powder. The cuttings were then left in a mist frame with bottom heat for 5 weeks. 80 cuttings were taking and were exposed to 4 treatments. 1) 20 cuttings, perlite & no rooting hormone powder 2) 20 cuttings, perlite & Stim-Root #1 3) 20 cuttings, Agromix (G6) & no rooting hormone powder 4) 20 cuttings, Agromix (G6) & Stim-Root #1 Results This experiment gave many interesting results; firstly, all the cuttings rooted and formed shoots. For root growth the perlite treatment had an average length of 4.95 cm, the perlite and Stim-Root had an average of 5.28 cm, G6 had an average of 7.7 cm and G6 and Stim-Root had an average of 12.93 cm. An ANOVA and Pairwise T test were performed and showed that the G6 with Stim-Root treatment produced roots that were significantly longer than the other treatments with 95% confidence. For shoot growth, perlite had an average growth of 3.85 cm, Perlite with Stim-Root 3.05 cm, G6 7.2 cm and G6 with Stim-Root 6.875 cm. Using the same statistical tests used for root growth, G6 and G6 with perlite were shown to be equal and their shoot growth was statistically greater than the perlite treatments. Conclusions Overall, the G6 with Stim -Root treatment produced the best root growth and G6 and G6 with Stim-Root both produced the most shoot growth. Therefor, G6 with Stim-Root had the best effect on overall plant growth. However, all the treatments had all their cuttings root and many of the Stim- Root treatments had indistinguishable results from their non- Stim-Root control. Therefor, growers should know that while G6 with Stim-Root was the best, Tahitian Bridal Veil stem cuttings seem to be able to grow in many different potting mixtures and rooting hormones improve rooting, but their effect is not very large, so whether it is cost effective to use these chemicals must be determined by the grower. Acknowledgements I would like to thank Dr. Danielle Donnelly for her vast knowledge that was essential in this experiment, Jonathan Sangiovani with his help with filming, and Guy Rimmer along with the rest of the Raymond Greenhouse staff for watering, supplies, and the maintenance of the greenhouse. References Acevedo-Rodriguez, P., and M.T. Strong. 2005. Monocotyledons and gymnosperms of Puerto Rico and the Virgin Islands. United States national herbarium. 52:1-415. January 26, 2018. https://botany.si.edu/pubs/CUSNH/vol52web.pdf Agro Mix G6. Fafard et frères. Accessed April 12, 2018. http://www.fafard.ca/wp-content/uploads/2016/03/Technical-Sheet-G6-AN.pdf Gibasis geniculata. Learn2Grow. January 26, 2018. http://www.learn2grow.com/plants/gibasis-geniculata/ Tahitian Bridal Veil (Gibasis geniculata). National gardening association. January 26, 2018. https://garden.org/plants/view/112508/Tahitian- Bridal-Veil-Gibasis-geniculata/ Stefancic, M., Stampar, F., and Osterc, G. 2005. Influence of IAA and IBA on root development and quality of Prunus GiSelA 5' leafy cuttings. Hortscience, 40:2052-2055. January 26, 2018. http://hortsci.ashspublications.org/content/40/7/2052.full.pdf Horticultural grade perlite. 1970. National bureau of standards, Washington, D.C., U.S. January 29, 2018. http://books.google.com/books?id=koRT8FBQfMUC. .
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