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Sgk: an Old Enzyme Revisited Sgk: an old enzyme revisited Nicolette Farman, … , Sheerazed Boulkroun, Nathalie Courtois-Coutry J Clin Invest. 2002;110(9):1233-1234. https://doi.org/10.1172/JCI17064. Commentary The elucidation of a key role for the serum- and glucocorticoid-regulated kinase (Sgk) in the regulation of the epithelial Na+ channel (ENaC) has led to substantial advances in the understanding of the signaling pathways involved in corticosteroid hormone action (1, 2). Indeed it is well established that the mineralocorticoid hormone aldosterone is a major regulator of body fluid homeostasis and blood pressure levels, mainly because of its capacity to modulate renal sodium reabsorption. However many aspects of this regulation pathway are not fully understood. Aldosterone binds to the mineralocorticoid receptor (MR), a member of the nuclear receptor superfamily which controls a network of genes in tight epithelia, ultimately leading to regulation of sodium entry into epithelial cells via the amiloride-sensitive ENaC (3). Most of the early steps of aldosterone action following MR-mediated transcriptional events are still incompletely elucidated. Differential hybridization studies have identified the serine/threonine kinase Sgk as a major transcriptional target of aldosterone. At least two other isoforms of Sgk, Sgk2 and Sgk3, have been identified after the initial characterization of Sgk (1), now referred to as Sgk1 (4). In this issue of the JCI, Wulff and colleagues (5) generated sgk1- deficient mice and examined the effects of dietary salt-restriction on renal water and electrolyte excretion. Sgk1 is a positive regulator of sodium transport Within one hour of aldosterone exposure, […] Find the latest version: https://jci.me/17064/pdf COMMENTARY See the related article beginning on page 1263. Sgk: an old enzyme revisited Nicolette Farman, Sheerazed Boulkroun, and Nathalie Courtois-Coutry Institut National de la Santé et de la Recherche Médicale U478, Institut Fédératif de Recherche 02, Université Paris 7, Faculté de Médecine Xaviér Bichat, Paris, France J. Clin. Invest. 110:1233–1234 (2002). doi:10.1172/JCI200217064. The elucidation of a key role for the leagues (5) generated sgk1-deficient expression system (6–10). It has also serum- and glucocorticoid-regulated mice and examined the effects of been shown that Sgk1 promotes an kinase (Sgk) in the regulation of the dietary salt-restriction on renal water increase in the number of active epithelial Na+ channel (ENaC) has led and electrolyte excretion. ENaCs at the surface of the oocyte to substantial advances in the under- (10–12). It was initially thought that standing of the signaling pathways Sgk1 is a positive regulator Sgk1 could phosphorylate the sodium involved in corticosteroid hormone of sodium transport channel itself, but several attempts action (1, 2). Indeed it is well estab- Within one hour of aldosterone expo- failed to confirm this hypothesis (13). lished that the mineralocorticoid hor- sure, Sgk1 is transcriptionally induced In fact, control of the number of mone aldosterone is a major regulator in both the amphibian A6 cell line and active ENaCs (including the regula- of body fluid homeostasis and blood mammalian tight epithelia, and tion of their surface expression) pressure levels, mainly because of its strongly stimulates sodium transport appears to depend on ENaC interac- capacity to modulate renal sodium when co-expressed with the three tion with the ubiquitin ligase Nedd4 reabsorption. However many aspects ENaC subunits — αENaC, βENaC, (13). Multiple experimental approach- of this regulation pathway are not and γENaC, in the Xenopus oocyte es have led to the notion that the fully understood. Aldosterone binds to the mineralocorticoid receptor (MR), a member of the nuclear recep- tor superfamily which controls a net- work of genes in tight epithelia, ulti- mately leading to regulation of sodium entry into epithelial cells via the amiloride-sensitive ENaC (3). Most of the early steps of aldosterone action following MR-mediated tran- scriptional events are still incomplete- ly elucidated. Differential hybridiza- tion studies have identified the serine/threonine kinase Sgk as a major transcriptional target of aldos- terone. At least two other isoforms of Sgk, Sgk2 and Sgk3, have been identi- fied after the initial characterization of Sgk (1), now referred to as Sgk1 (4). In this issue of the JCI, Wulff and col- Address correspondence to: Nicolette Farman, INSERM U478, Faculté de Médecine Figure 1 Xaviér Bichat, 16 rue Henri Huchard, BP 416, Mechanism of ENaC expression in an aldosterone-sensitive epithelial cell. (a) In a resting state, few 75870 Paris Cedex 18, France. ENaCs, which facilitate sodium reabsorption in a rate-limiting fashion, are resident in the apical Phone: 33-1-44-85-63-23; membrane. Factors known to enhance ENaC surface expression and activity are counterbalanced Fax: 33-1-42-29-16-44; by retrieval of these channels from the membrane through the ubiquitination pathway mediated E-mail: [email protected]. by Nedd4-2. (b) Shortly after aldosterone exposure and binding to the MR, transcriptional stim- Conflict of interest: No conflict of interest ulation of Sgk1 leads to phosphorylation of Nedd4-2, which subsequently disrupts ENaC/Nedd4-2 has been declared. Nonstandard abbreviations used: serum- interactions. In this situation, ubiquitination of ENaCs is reduced, thus favoring ENaC residence and glucocorticoid-regulated kinase (Sgk); in the apical membrane and enhanced sodium reabsorption. Afterward, stimulation by aldosterone epithelial Na+ channel (ENaC); requires successive activation of several genes resulting in sustained sodium reabsorption across mineralocorticoid receptor (MR); the epithelium. PI3K, phosphatidylinositol-3′-kinase; ERK, extracellular signal-regulated kinase; pseudohypoaldosteronism (PHA). PKA, protein kinase A; Na/K-ATPase, sodium/potassium ATPase. The Journal of Clinical Investigation | November 2002 | Volume 110 | Number 9 1233 interaction of the WW domains of They showed that the amiloride-sensi- data provide support for the notion Nedd4 with PY motifs in the C-termi- tive transepithelial potential differ- that hypertension and insulin resist- nal intracellular tail of the three ENaC ence, measured in an isolated perfused ance are mechanistically linked. subunits is critical for retrieval of tubule setting, appeared to be reduced these channels from the epithelial cell in Sgk1 mutant mice, indicative of 1. Webster, M.K., Goya, L., Ge, Y., Maiyar, A.C., and membrane, thus reducing the capaci- impaired sodium transport in the Firestone, G.L. 1993. Characterization of sgk, a ty of these cells to reabsorb sodium. absence of Sgk1. However immuno- novel member of the serine/threonine protein kinase gene family which is transcriptionally Interestingly, it has recently been fluorescence revealed that ENaCs were induced by glucocorticoids and serum. Mol. Cell demonstrated that channel retrieval still present within the apical mem- Biol. 13:2031–2040. from the cell membrane is prevented brane, strongly suggesting that factors 2. Pearce, D. 2001. The role of SGK1 in hormone- regulated sodium transport. Trends Endocrinol. by the phosphorylation of Nedd4-2, other than Sgk1 are involved in ENaC Metab. 12:341–347. an isoform of Nedd4, by Sgk1 (13, 14). maintenance at this site. 3. Stockand, J.D. 2002. New ideas about aldos- Besides direct evidence that Sgk1 In conclusion, the authors have terone signaling in epithelia. Am. J. Physiol. 282:F559–F576. indeed phosphorylates Nedd4-2, it unmasked a salt-wasting syndrome by 4. Kobayashi, T., Deak, M., Morrice, N., and Cohen, was also shown that this event impairs imposing a forced situation — a sodi- P. 1999. Characterization of the structure and reg- Nedd4-2 interaction with the ENaC, um-free diet — upon sgk1–/– mice. ulation of two novel isoforms of serum- and glu- cocorticoid-induced protein kinase. Biochem. J. leading to an increase in ENaC surface These results justify consideration of 344:189–197. expression and activity (13–15). Thus sgk1 as a potential causal gene in some 5. Wulff, P., et al. 2002. Impaired renal Na+ reten- Sgk1 and Nedd4-2 exert opposite forms of pseudohypoaldosteronism tion in the sgk1-knockout mouse. J. Clin. Invest. 110:1263–1268. doi:10.1172/JCI200215696. effects on the ENaC: Sgk1 stimulates (PHA). Indeed, mutations in ENaC 6. Chen, S.Y., et al. 1999. Epithelial sodium channel ENaC membrane expression and subunits (16) or the MR (17) have been regulated by aldosterone-induced protein sgk. activity in response to aldosterone, evidenced in some cases of PHA, but Proc. Natl. Acad. Sci. USA. 96:2514–2519. 7. Naray-Fejes-Toth, A., Canessa, C., Cleaveland, while Nedd4-2 reduces it (Figure 1). no common genetic defect has been E.S., Aldrich, G., and Fejes-Toth, G. 1999. sgk is determined. Along the same line, an aldosterone-induced kinase in the renal col- Genetic disruption recent evidence (18) supports a critical lecting duct. Effects on epithelial Na+ channels. J. Biol. Chem. 274:16973–16978. of sgk1 generates role for WNK (with no lysine) kinases 8. Shigaev, A., Asher, C., Latter, H., Garty, H., and a mild salt-wasting syndrome in the regulation of sodium transport Reuveny, E. 2000. Regulation of sgk by aldos- Contrary to expectations, the report by in the distal nephron, as highlighted terone and its effects on the epithelial Na(+) channel. Am. J. Physiol. 278:F613–F619. Wulff et al. (5) revealed that there was by WNK kinase mutations in a rare 9. Brennan, F.E., and Fuller, P.J. 2000. Rapid upreg- no major abnormality in blood pres- form of hypertension, pseudohypoal- ulation of serum and glucocorticoid-regulated sure and fluid handling in sgk1–/– mice. dosteronism type II, also known as kinase (sgk) gene expression by corticosteroids in –/– vivo. Mol. Cell Endocrinol. 166:129–136. However, when sgk1 mice were placed Gordon Syndrome. 10. Bhargava, A., et al. 2001. The serum- and gluco- on a low-salt diet, their ability to retain corticoid-induced kinase is a physiological sodium was greatly impaired. The Phenotypic compensation raises mediator of aldosterone action. Endocrinology. 142:1587–1594.
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