Banting and Best: the Extraordinary Discovery of Insulin
106 Rev Port Endocrinol Diabetes Metab. 2017;12(1):106-115 Revista Portuguesa de Endocrinologia, Diabetes e Metabolismo www.spedmjournal.com Artigo de Revisão Banting and Best: The Extraordinary Discovery of Insulin Luís Cardosoa,b, Dírcea Rodriguesa,b, Leonor Gomesa,b, Francisco Carrilhoa a Department of Endocrinology, Diabetes, and Metabolism, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal b Faculty of Medicine of the University of Coimbra, Coimbra, Portugal INFORMAÇÃO SOBRE O ARTIGO ABSTRACT Historial do artigo: Diabetes was a feared disease that most certainly led to death before insulin discovery. During the first Recebido a XX de XXXX de 201X two decades of the 20th century, several researchers tested pancreatic extracts, but most of them caused Aceite a XX de XXXX de 201X Online a 30 de junho de 2017 toxic reactions impeding human use. On May 1921, Banting, a young surgeon, and Best, a master’s student, started testing the hypothesis that, by ligating the pancreatic ducts to induce atrophy of the exocrine pancreas and minimizing the effect of digestive enzymes, it would be possible to isolate the Keywords: internal secretion of the pancreas. The research took place at the Department of Physiology of the Diabetes Mellitus University of Toronto under supervision of the notorious physiologist John MacLeod. Banting and Insulin/history Best felt several difficulties depancreatising dogs and a couple of weeks after the experiments had Pancreatic Extracts/history begun most of the dogs initially allocated to the project had succumbed to perioperative complications. When they had depancreatised dogs available, they moved to the next phase of the project and prepared pancreatic extracts from ligated atrophied pancreas.
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