FACULTY FULL NAME AMAL MAHMOUD HUSSEIN SALEM

POSITION Professor of Bioinformatics and Molecular Virology

Personal Data

Nationality |Egyptian Date of Birth |12/1/1968 Department |Biology Official Email | [email protected] Language Proficiency

Language Read Write Speak Excellent Excellent Excellent English Excellent Excellent Excellent French Excellent Good Very good

Academic Qualifications (Beginning with the most recent)

Date Academic Degree Place of Issue Address 1990 BSc University 1994 MSc -ORSTOM Egypt- 2001 PhD Cairo University-CIRAD Egypt-France

PhD, Master or Fellowship Research Title: (Academic Honors or Distinctions)

PhD Biological and molecular characteristics of maize yellow stripe virus and its relationship with the leafhopper vector Master Characterization and serology of the leafhopper-borne maize yellow stripe tenuivirus in Egypt Fellowship Virus characterization and diagnosis, ORSTOM (Egypt-French project) Fellowship Molecular characterization and Bioinformatics analysis of MYSV (CIRAD Montpellier, France) Fellowship Bioinformatics analysis of CTV and its population structure (University of Davis-California, USA)

Professional Record: (Beginning with the most recent)

Job Rank Place and Address of Work Date Professor Imam Abdulrahman Bin Faisal Saudi Arabia 25-9-2019 Univ. Associate trainer certified by IBCT 19-9-2018 Associate professor Imam Abdulrahman Bin Faisal Saudi Arabia 3-1-2017 Univ. Head of Bioinformatics Dept. GEBRI-Sadat Univ. Egypt 2015-2016 Director manger Alkhwarizmi Center for Egypt 2013 Bioinformatics, Egypt

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Associate professor GEBRI-Sadat Univ. Egypt 2012 Assistant professor GEBRI-Sadat Univ. Egypt 2005 Lecturer GEBRI-Sadat Univ. Egypt 2002 Post-Doc fellow UC-Davis USA 2001 Assistant lecturer Plant protection ins. Ministry of Egypt 1998 agric. PhD fellowship CIRAD-Montpellier France 1995 MSc fellowship French-Egyptian project Egypt 1991 (ORSTOM)

Administrative Positions Held: (Beginning with the most recent)

Administrative Position Office Date Head of Bioinformatics Dept. GEBRI-Sadat Univ., Egypt 2015 Director manger Alkhwarizmi Center for Bioinformatics, Egypt 2013 Head, community service committee Dept. Biology, College of Science, Imam Abdulrahman 2017 Bin Faisal Univ., KSA Head, administrative system committee GEBRI-Sadat Univ., Egypt 2015-2016

Scientific Achievements

Refereed Scientific Research Papers Accepted for Publication

Published Refereed Scientific Researches (In Chronological Order Beginning with the Most Recent)

1. Zidan, A. M., Saad, E. A., Ibrahim, N. E., Mahmoud, A., Hashem, M. H., Hemeida, A. A. (2020). PHARMIP: An insilico method to predict genetics that underpin adverse drug reactions. Methods. 7:1-14. 2. Elhefnawi, M. M., Hasan, M. E., Mahmoud, A., Khidr, Y. A., El-Absawy, E. A., Hemeida, A. A. (2019). Prediction and analysis of three dimensional structure of the p7-transactivated protein1 of hepatitis C virus. Infectious Disorders - Drug Targets. 19(1):55-66. 3. Essam M Janahi; Aboul-Ata E Aboul-Ata; I M El-Kalamawy; Amal Mahmoud; M S El-Ghanam; Kathleen Hefferon. (2017). HBV Genome, HbsAg Isolation and Virotherapy Innovation Using Plant System in the Middle East. HEPATITIS: A GLOBAL HEALTH CONCERN. Vol 2 Chapter 1 P 1-19. ISBN: 978-93-87500-05-1. 4. Elhefnawi, M. M., Hasan, M. E., Mahmoud, A., Khidr, Y. A., El-Absawy, E. A., Hemeida, A. A. (2017). In silico discovery of novel hepatitis c virus p7 transactivated protein1inhibitor by using structure-based virtual screening. J. Product. & Dev., 22(1): 103-119. 5. Mohamed Ezz, Amal Mahmoud, Fawzy A. El-Feky and Alaa A. Hemeida (2017). Searching and Analyzing for a Different Lengths of Microsatellite Repeats in the Completed Genome of Phoenix dactylifera Chloroplast, by Using Regular Expression builder –Language. International Journal of Science and Research (IJSR). 6 (8): 1296-1301. 6. El-aarag, S. Mahmoud, A., Hashem, M. H., Abd Elkader, H., Hemeida, A.E. and ElHefnawi M. (2017). In silico identification of potential key regulatory factors in smoking-induced lung cancer. BMC Medical Genomics 10:40. د. امال محمود.)2017(. المعلوماتيه الحيويه...افاق واعده في تشخيص االمراض. مجلة التقدم العلمي . مجلد 97 . .7 8. Zidan A. M.; Alaa A. Hemeida and Amal Mahmoud (2016). The target to mediate Nitazoxanide's anti-HCV effect. In-silico study. Edition: 1st, Publisher: LAMBERT academic publishing, ISBN: 978-3-659-92424-8

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9. Nadia S. El-Nahas, Gehan S. Sadek, Amal Mahmoud, Amany A. Rady, Salwa, F. Oshiba and Reham M. Barakat. (2015). Immunological, histopathological and immunohistochemical responses to a new B cell epitope-based vaccine against leishmaniasis in experimental mice. Journal of American Science 11(7). 10. Amal Mahmoud and Medhat H. Hashem (2014). Bioinformatics analysis of polymerase and envelope genes of Hepatitis B virus in Egypt. Egyptian J. Virol. 11 32-37. 11. Zidan, A. M., Hemeida, A. A., Mahmoud, A., Hashem, M. H., (2013). Glycogen Synthase Kinase 3 B prediction as primary cellular target to mediate anti- Hepatitis C effect of Nitazoxanide. 12. Ibrahim, A. I , Hemida A. A., Amal M. Hussain, Bekhit, M. and Amal , A. Zweil. (2013). Comparative Studies On Catharanthus sp. Cultivars using Chlorophyll Content and Peroxidase Isozyme. J. product. & Dev., 18(1):77-84. 13. El-Absawy, E.A , Khidr, Y.A. Mahmoud, A., Hasan, M.E. and Hemeida, A.A. (2012). Somatic Embryogenesis and Plant Regeneration Induced from Mature Seeds, Cotyledons and Shoot Tips of Cucumber. Journal of Productivityand Development. J. Product. & Dev., 17(2): 45- 61. 14. Khidr, Y.A. Mahmoud, A. El-Absawy, E.A. Hemeida, A.A. and Hasan, M.E. (2012). Production of Transgenic Cucumber Plantlets containing Sequences from Watermelon Mosaic Virus-II fused with GFP Gene. Egypt. J. Genet. Cytol. 41(1):19-35. 15. Mahmoud, A. (2012). Comparative sequence analysis and epitope prediction of the partial glycoprotein G gene of bovine ephemeral fever virus isolate from Egypt. Egyptian J. Virol. 9 (1) 49-58. 16. Amal Mahmoud and Medhat H. Hashem. (2012). Phylogenetic subtyping of hepatitis C virus 5' UTR isolated in Egypt and the effect of 2 transitions in subdomain IIId on the apical loop structure. Life Science J. 9(1):903-909. 17. Zaghloul, A. H., Mahmoud, Amal, Hassan, H. Y., Hemeida, A. A., Nayel, M. A. and Zaghawa, A. A. (2012). Establishment of dot-blot hybridization for diagnosis of bovine ephemeral fever virus in Egypt. International J. Virol. 8 (3) 271-278. 18. El-Absawy, E.A; Amal Mahmoud, A.A. Hemeida and M. Helmy (2012). Molecular variation of Potato virus Y isolated from Egypt. International J. Virol. 8 (1) 81-89. 19. Hemeida, A. A.; M. Osman; Mohamed El-Shahat; Medhat H. Hashem; Amal Mahmoud and Hosni Dahi (2011). Genetic variations in a conserved 5'-Untranslated region of hepatitis C virus isolated from Egypt. International J. Virol, 7(3) 91-99. 20. Ibrahim A. I, Hemeida A. A, Nower, A. A., Amal Mahmoud, Salwa N. Zein and Asmaa abdelmordy (2009). Antiserum production and molecular detection of Potato virus X and Potato virus Y. Egyptian J. Virol., 6:273- 285. 21. El-Araby, W., S., Ibrahim, I., A., Hemeida, A., A., Mahmoud, Amal, Soliman, A. M. El-Attar, A. K., and Mazyad, H. M. (2009). Biological, serological and molecular diagnosis of three major potato viruses in Egypt. International journal of virology 5 (2): 77-88. 22. Ammar E.-D., Aboul-Ata A. E.and Mahmoud, A. (2009). Maize virus diseases, “in Virus Diseases affecting crops in the Arab world”. Beirut, Lebanon, 970 pp. 23. Mahmoud A., Royer M., Granier M., Ammar E.-D. and Peterschmitt M. (2007). High genetic identity between RNA 3 segment of an Old World isolate and a New World isolate of Maize stripe virus. Arch Virol 152: 1583-1586. 24. Mahmoud A., Royer M., Granier M., Ammar E.-D., Thouvenel J.-C. and Peterschmitt M. (2007). Evidence for a segmented genome and partial nucleotide sequences of Maize yellow stripe virus, a proposed new tenuivirus. Arch Virol 152: 1757-1762. 25. Ammar E.-D., Khlifa E. A., Mahmoud A., Abol-Ela S. E. and Peterschmitt M. (2007). Evidence for multiplication of the leafhopper-borne maize yellow stripe virus in its vector using ELISA and dot-blot hybridization. Arch Virol 152: 489-494.

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26. Mahmoud A. (2001). Ph.D. (Biological and molecular characteristics of maize yellow stripe virus and its relationship with the leafhopper vector). M.Sc. thesis, Cairo University, pp154. 27. Mahmoud A. H., Thouvenel J. C., Abol Ela S. E., Sewify G. H. and Ammar E. D. (1996). Detection of maize yellow stripe tenui-like virus by ELISA and dot-blot tests in host plants and leafhopper vector in Egypt. Phytopath. medit. 35, 19-23. 28. Thouvenel J. C., Abol Ela S. E., Sewify G. H., El-Hariry M., Mahmoud A. H. and Ammar E. D. (1996). Characterization and serology of the leafhopper-borne maize yellow stripe tenuivirus in Egypt. Bull. Fac. Agric., Cairo Univ. 47, 179-190. 29. Amal Mahmoud Hussein et Laurent Bigarre. (1995). Diagnostic du virus des rayures jaunes du mais en Egypte. Nouvelles scientifiques. PP 78. 30. Mahmoud A. (1994). M. Sc. (Characterization and serology of the leafhopper-borne maize yellow stripe tenuivirus in Egypt). Ph.D. thesis, Cairo University, pp75.

Scientific Research Papers Presented to Refereed Specialized Scientific Conferences

1. Mahmoud A. H., Royer M., Granier M., Ammar E. D., Thouvenel J. C. and Peterschmitt M. (1997). Biological and molecular characterization of the leafhopper-borne maize yellow stripe tenuivirus from Egypt. In: Societe Francaise de Phytopathologie., Proceedings of the 10th congress of the mediterranean phytopathological union. June 1st-5th, 1997, Montpellier, France. P. 349-350. 2. Mahmoud A., Granier M., Peterschmitt M., Abul-Ela S. and Ammar E. D. (2001). Multiplication of maize yellow stripe virus in its leafhopper vector, Cicadulina chinai. First conference on Integrated Pest Management (IPM) At the college of Agriculture, Cairo University, Giza, Egypt on 22nd-23rd April, 2001.

3. Mahmoud A., Sheta H., Grafton-Cardwell E., polek M., Gumpf D., Falk B. and Ullman D. (2002). The role of aphid transmission in Citrus tristeza virus population structure. The American society of virology (ASV) meeting. University of Kentucky, USA on 21-24 July, 2002. 4. Diane E. Ullman, Amal Salem, Hany Sheta and Bryce W. Falk. (2002). Is aphid transmission of Citrus tristeza virus (CTV) a genetic bottleneck?. The 2002ESA Annual Meeting and Exhibition Fort Lauderdale, FL. USA, November, 2002. 5. Ammar E.D., Mahmoud A., Khlifa E., Granier M., Peterschmitt M. (2003). Evidence for multiplication of the leafhopper-borne maize yellow stripe tenui-like virus in its vector and suggestion of a new genus for tenuiviruses [Abstract]. In : Advances in plant virology : A three day International conference at CIRAD, Montpellier, France on 29 September - 1 October 2003. Wellesbourne : Association of Applied Biologists, 1 p.. International Conference : Advances in Plant Virology, 2003-09-29/2003-10-01, Montpellier, France. 6. Ibrahim, A. I., Mazyad, H. M., Hemeida, A. A., Amal, Mahmoud, Soliman, A. M.; El-Attar, A. K. and El-Araby, W. S. (2008). Biological studies, serological and molecular diagnosis of three major potato viruses in Egypt. The Second International Conference of Virology "Emerging and Exotic Viral Infection, Challenging Threats of Human, Animal and Plant Health" 5-6 April, 2008, pp. 24. 7. Ibrahim, A. I., Mazyad, H. M., Hemeida, A. A., Amal, Mahmoud, Soliman, A. M., El-Attar, A. K. and El-Araby, W. S. (2008). Comparative study for the detection of three major potato viruses using several molecular and serological methods. “New Life Sciences: From Promises to Practice” Bio Vision 12-16 April 2008, poster presenter's pp. 2. 8. Zaghloul, A. H., Amal Mahmoud, Hassan, H. Y., Hemeida, A. A., Zaghawa, A. A. and Nayel, M. (2010). Establishment of Dot-Blot Hybridization For Diagnosis of Bovine Ephemeral Fever Virus In Egypt.

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The 3rd international conference of the Egyptian society of virology "Current challenge of viral diseases". Cairo University, Egypt on 24-25 November, 2010. 9. Hemeida, A. A.; M. Osman; Mohamed El-Shahat; Medhat H. Hashem; Amal Mahmoud and Hosni Dahi. (2010). Genetic variations in a conserved 5-untranslated region of hepatitis C virus genome isolated from Sohag governate-Egypt. The 3rd international conference of the Egyptian society of virology "Current challenge of viral diseases". Cairo University, Egypt on 24-25 November, 2010. 10. El-Absawy, E. A., Amal Mahmoud, Hemeida, A. A., and Helmy, M. (2010). Molecular variation of Potato virus Y isolated from Egypt. The 3rd international conference of the Egyptian society of virology "Current challenge of viral diseases". Cairo University, Egypt on 24-25 November, 2010. 11. Amal Mahmoud, Maria Tsompana, Hany Sheta, Elizabeth Grafton-Cardwell, MaryLou Polek, J. Gumpf, D, and Diane E. Ullman (2012). Aphid Transmission of Citrus tristeza virus Reduces Nucleotide Diversity and Results in Genotype Alteration and Fixation of New Amino Acid Substitutions in the Leader Proteinase Domain L2. The 4th International Conference of Virology "Towards strategy fo controlling viral pathogens". Sharm El- Sheikh, Egypt on 14-16 November, 2012, pp. 67. 12. Amal Mahmoud and Medhat H. Hashem (2014). Bioinformatics analysis of the S Gene of Hepatitis B Virus in Egypt. The 5th international conference of the Egyptian society of virology "Viruses and their potential hazard to human, animal and plant, the role of national & international organization". Hurghada, Egypt on 9-12 December, 2014. 13. Amal Mahmoud, Reham M. Barakat, Nadia S. El-Nahas, Gehan S. Sadek, Amany A. Rady and Salwa F. Oshiba. (2016). Evaluation of Immune Response Against Leishmaniasis in Vaccinated Experimental Mice by Different Antigenic Epitopes. 1st international conference of Genetic Engineering and Biotechnology Research Institute” Integrative science and biotechnology”. Sharm El-Sheikh, Egypt on 26-29 April, 2016. 14. Mohamed Ezz, Amal Mahmoud, Fawzy El-Tohamy and Alaa A. Hemeida (2016). Detection and Comparative Analysis of the Tandem Repeats Motifs in the Completed Genomes of Human Coronavirus (Hku1) By Using Regular Expression Finder Tool. The 6th international conference of the Egyptian society of virology " Viral risks and environmental changes, the role of national & international organization". Hurghada, Egypt on 29November-2 December, 2016. 15. Mahmoud M. El Hefnawi, Mohamed E. Hasan, Amal Mahmoud, Yehia A. Khidr, El-Sayed A. El-Absawy and Alaa A. Hemeida (2016). 3-D structure prediction and analysis of the p7-transactivated protein1 of hepatitis C virus. The 6th international conference of the Egyptian society of virology " Viral risks and environmental changes, the role of national & international organization". Hurghada, Egypt on 29November-2 December, 2016.

Current Researches

# Research Title Name of Investigator(s) 1 Antiviral treatment: Prediction of vaccines and drugs against Mahmoud Amal, Elhefnawi, M. M., Zidan, coronavirus disease-2019(COVID-19) A. M., Mostafa A, Ahmad M, Hussein S, Covid19-2020-001-BASRC Shehata M, Alaarag S 2 Bioinformatics analysis of bacterial alkaline Proteases in Saudi Amal Mahmoud, Amany Alqosaibi and Arabia Essam Kotb 3 Project: Production of pathogen-free minitubers of potato in Ibrahim Abd Almaksoud, Taymoor and New Wadi governorate Amal Mahmoud 4 Amal Mahmoud, Sahar Eldousary, Ekbal Molecular identification of populations of bacteria associated Khedr

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with samples of stored rice in Saudi Arabia

Membership of Scientific and Professional Societies and Organizations • Member, Egyptian Society of Virology (ESV) • Member, scientific projects committee

Teaching Activities

Undergraduate

# Course/Rotation Title No./Code Extent of Contribution (no. of lectures/Tutorials. Or labs, Clinics) 1 Cytology BIOL 142N 13 lectures 2 Cell biology BIOL 206 Lab advisor 3 Parasitology BIOL 343N Lab advisor 4 Immunology BIOL 442N Lab advisor 5 Molecular Biology BIOL 401N 14 Lectures/ Lab advisor 6 Graduation project BIOL 448N 14 meetings 7 Molecular Biology GENETC 308 14 Lectures/ Lab advisor 8 Micro techniques Lab advisor 9 Bioinformatics BIOL401 15 lectures/ Lab advisor 10 Principle of Genetics GENETC 304 14 lectures/lab advisor 11 Virology MICRO424 14 lectures 12 Gene regulation GENTC 420 14 lectures

Postgraduate

# Course/Rotation Title No./Code Extent of Contribution (no. of lectures/Tutorials. Or labs, Clinics) 1 Advanced Bioinformatics ZOOL 624 14Lectures/14 Labs 2 Advanced physiological microbiology MICRO 574 10 Labs 3 Nucleic acid sequence analysis (MSc) B8-10 Lectures/labs 4 Proteomics (PhD) B8-21 Lectures/labs 5 Protein sequence analysis (MSc) B8-20 Lectures/labs 6 Sequence analysis (PhD) B8-22 Lectures/labs 7 Bioinformatics (MSc) B8-2 Lectures/labs 8 Seminars (MSc/PhD) B8-24 Lectures 9 Special topics (MSc/PhD) B8-23 10 Molecular methods in virology - Lectures 11 Advanced and general virology - Lectures 12 Fundamentals of plant virology - Lectures 13 Plant diseases - Lectures 14 Transgenic plants Lectures 15 Diagnosis and control of plant diseases Lectures

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Bioinformatics BIOL402 Lectures

Course Coordination

# Course Title and Code Coordination Co-coordination Undergrad. Postgrad. From to 1 Cytology- BIOL 142N yes - Yes - 2017 2018 2 Advanced physiology of Practical - - yes 2017 2017 microbiology coordination 3 Molecular Biology Practical - Yes - 2017 2018 coordination 4 Bioinformatics Yes - Yes - 2018 2019 5 Bioinformatics Yes - - Yes 2019 2020 6 Advanced Yes - - Yes 2020 2020 Bioinformatics

Supervision of Master and/or PhD Thesis

# Degre Title Institution Date e Type 1 PhD Detection and Comparative Analysis for the Bioinformatics department, GEBRI, 2019 Tandem Repeats Motif / Microsatellite Repeats Sadat University in the Completed Genomes by Using Regular Expression Finder Tool 2 PhD Functional genomic analysis for stem rust Bioinformatics department, GEBRI, 2019 resistance in durum wheat (T.turgidum L. subsp. Sadat University Durum) 3 PhD Protein structure prediction by Bioinformatics Bioinformatics department, GEBRI, 2018 tools Sadat University 4 PhD Immunological, histopathological and Faculty of medicine, Menoufia 2015 immunohistochemical responses to a new B University cell epitope-based vaccine against leishmaniasis in experimental mice 5 MSc Computational prediction of the protein kinase Bioinformatics department, GEBRI, 2014 Sadat University that mediates the anti-HCV effect of nitazoxanide 6 MSc In silico identification of drug targets in lung Bioinformatics department, GEBRI, 2014 cancer Sadat University 7 MSc Studies on molecular variations of potato virus X Plant Biotechnology department, 2012 and Y in Egypt GEBRI, Sadat University 8 MSc In Vitro Production Of Specific Pathogen Tested Plant Biotechnology department, 2010 Potato Plants GEBRI, Sadat University 9 MSc Development and Standardization of Rapid and Plant Biotechnology department, 2008 Sensitive viral Detection Methods Based on GEBRI, Sadat University Molecular Biology for the Diagnosis of Some Potato Viruses

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Ongoing Research Supervision

# Degre Title Institution Date e Type 1 MSc Studies on keratinase gene of some keratinolytic Department of Biology, Faculty of 2020 science, Imam Abdulrhman Bin Faisal bacteria in the Eastern Province of Saudi Arabia University 2 PhD Bioinformatics and Pharmacogenomics in Bioinformatics department, GEBRI, 2015 Sadat University personalized cancer medicine Introduction

3 PhD Genetic guided clinical pharmacy: right drug, Bioinformatics department, GEBRI, 2015 Sadat University right dose, and right time prediction based on patient's genetic profile.

Administrative Responsibilities, Committee and Community Service (Beginning with the most recent) Administrative Responsibilities

# From To Position Organization 1 2015 Head of Bioinformatics Dept. GEBRI-Sadat University, Egypt 2016

Committee Membership

# From To Position Organization 1 2017 Present Member, postgraduate and scientific research Dept. Biology, College of Science, committees Immam Abdulrahman Bin Faisal Univ., KSA 2 2016 2016 Member-Scientific projects committee GEBRI-Sadat Univ. Egypt 3 2016 2016 Member-Course specification-dept. bioinformatics GEBRI-Sadat Univ. Egypt committee 4 2015 2015 Member-Course specification-dept. plant GEBRI-Sadat Univ. Egypt biotechnology committee 5 2015 2016 Head, community service committee GEBRI-Sadat Univ. Egypt

Scientific Consultations

# From To Institute Full-time or Part- time 1 2016 2016 Society and environment committee-Sadat Univ-Egypt Part-time 2 2013 2016 Alkhwarizmi for Bioinformatics Part-time 3 2005 2005 Viral diagnosis-Farm fries company- Sadat Univ-Egypt Part-time

Volunteer Work

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# From To Type of Volunteer Organization 2013 2019 Personal skills topics Facebook

Personal Key Competencies and Skills: (Computer, Information technology, technical, etc.)

1 Online training course, educational videos 2 Personal skills

Last Update

13/12/2020

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