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IKAP Antibody (Pab) 21.10.2014IKAP antibody (pAb) Rabbit Anti -Human/Mouse IKK Complex Associated Protein (IKBKAP) Instruction Manual Catalog Number PK-AB718-2337 Synonyms IKAP Antibody: IKK complex-associated protein, IKBKAP Description IKAP was initially identified as a scaffold protein of the I κB kinase complex that could bind to IKK α, IKK β, NF-κB, and the NF-κB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist. Quantity 100 µg Source / Host Rabbit Immunogen IKAP antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human IKAP. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, ICC, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually.h application have to be determined indivudually. IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 1 µg/mL. For immunofluorescence start at 20 µg/mL. Images Available upon request. References Cohen L, Henzel WJ, and Baeuerle PA. IKAP is a scaffold protein of the IkB kinase complex. Nature 1998; 395:292-6. Krappmann D, Hatada EN, Tegethoff S, et al. The I kappa B kinase (IKK) complex is tripartite and contains IKK gamma but not IKAP as a regular component. J. Biol. Chem. 2000; 275:29779-87. Anderson SL, Coli R, Daly IW, et al. Familial dysautonomia is caused by mutations of the IKAP gene. Am. J. Hum. Genet. 2001; 68:753-8. Hawkes NA, Otero G, Winkler GS, et al. Purification and characterization of the human elongator complex. J. Biol. Chem. 2002; 277:3047-52. Images Available upon request. Related Products IKAP Peptide, Cat. No. PK-AB718-2337P A20 Cell Lysate, Cat. No. PK-AB718-1288 FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES. PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014 .
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