bioRxiv preprint doi: https://doi.org/10.1101/469072; this version posted November 14, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Enzymatic and structural characterization of the Naegleria fowleri glucokinase 2 3 Jillian E. Milanes1, Jimmy Suryadi1, Jan Abendroth2, Wesley C. Van Voorhis3, Kayleigh F. 4 Barrett3, David M. Dranow2, Isabelle Q. Phan4, Stephen L. Patrick1, Soren D. Rozema5, 5 Muhammad M. Khalifa5, Jennifer E. Golden5, and James C. Morris1# 6 7 8 1Eukaryotic Pathogens Innovation Center 9 Department of Genetics and Biochemistry 10 Clemson University 11 Clemson, SC 29634 12 13 2CrystalCore 14 Beryllium Discovery 15 Bainbridge Island, WA 98110 16 17 3Center for Emerging and Re-emerging Infectious Diseases 18 Division of Allergy and Infectious Diseases, Department of Medicine 19 University of Washington 20 Seattle, WA 98109 21 22 4Seattle Structural Genomics Center for Infectious Disease 23 Center for Global Infection Disease Research 24 Seattle Children’s Research Institute 25 Seattle, WA 98109 26 27 5School of Pharmacy 28 Pharmaceutical Sciences Division 29 University of Wisconsin-Madison 30 Madison, WI 53705 31 32 33 Running title: Crystal structure of the glucokinase from the human pathogen N. fowleri 34 35 #Address correspondence to: James C. Morris, PhD, 249 LSB, 190 Collings Street, 36 Clemson SC 29634. FAX: (864) 656-0393; Email:
[email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/469072; this version posted November 14, 2018.