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YMJ-4-2015-En.Pdf CONTENTS Original Researches Kurtanov H.A., Danilova A.L., Yakovleva A.E., Gerasimova V.V., Savvina A.D., Maksimova N.R. Molecular and genetic testing of HLA II class genes in celiac disease patients in Yakutia Bashirov E.V., Dedu T.V., Duglas N.I. The outcomes of different organ preserving treatment of uterine fibroids Khanzadyan M.L., Demura T.A., Polina M.L. The characteristics of the connective tissue of the pelvic floor at the collapse of the genitals Gasanova B.M., Duglas N. I. Features of cytokine status of pregnant women with chronic pyelonephritis, depending on the placenta morphological characteristics Vakhnenko A.A., Skurikhina V.P., Masalskaya E.V., Dolbnya N.R., Danilova A.I., Skurikhina N.N., Bataeva V.V. Diseases of the biliary system in patients with overweight Timofeeva A.V., Mikhailova A.E., Zakharova R.N., Vinokurova S.P., Klimova T.M., Fedorova V.I., Baltakhinova M.E., Fedorov A.I. The functional state of the cardiovascular system of the NEFU named after M.K. Ammosov the I course students - girls Dorovskikh V.A., Lee O.N., Simonova N.V., Shtarberg M.A., Bugreeva T.A. Remaxol in correction of lipid peroxidation of biological membranes induced by cold exposure Korkin A.L., Hasanova S.V. The incidence of ulcerogenesis exogenous factors in patients with newly diagnosed stomach ulcers, complicated with bleeding and perforations Vinokurov M.M., Savelyev V.V., Gogolev N.M., Yalynskaya T.V. Two-level immunocorrection therapy of acute destructive pancreatitis in a multidisciplinary surgical hospital The methods of diagnostics and treatment Vasiliev S.P., Pavlov R.N. The new device and method for the treatment of congenital clubfoot in infants Cheremkina A.S., Ushnickij I.D., Akhremenko Ya.A., Nikiforova E.Yu., Tarasova L.A., Prokop'ev I.A. Characteristics of the “Yagel” preparation antimicrobial activity at the edge gingival inflammation Pavlov S.S., Pal’shin G.A. Experience in the use of rivaroxaban in the prevention of thromboembolic complications after endoprosthesis of large joints Health care, medical education and science organization Bogachevskaja S.A., Bogachevskij A.N. The level of availability of high-tech medical care for acquired heart defects in the RF and the FEFD. Trends over the last 10 years Borisova E.A., Savvina N.V., Lutskan I.P., Timofeev L.F. Effective contract as a method of optimization and growth of incomes of medical workers Ljadova M.V. Analysis of medical-legal questions assessing the quality of emergency aid to for the injured with the musculoskeletal system lesion Aprosimov L.A., Chichahov D.A. Dynamics of pediatric service staffing of the RS (Ya) Zasimova E.Z., Golderova A.S., Okhlopkova E.D., Tikhonova O.G., Ratushnyak S.S. Ratushnyak E.T. Medical and social characteristics of Water Transport Workers of the RS (Ya) Hygiene, sanitation, epidemiology and medical ecology Dogorova O.E., Vinokurova M.K. Characteristics of newly identified patients with infiltrative pulmonary multidrug-resistant tuberculosis in the RS (Ya) Ljalina L.V., Danilova M.A., Glushkevich V.A., Fedulova A.G., Afanas'eva A.N. The incidence of rotavirus infection in different regions of the RF in the pre-vaccination period Ivanova O. S., Maximova S.S., Donskaja A.A, Semenov S.I. Life quality of patients with viral hepatitis and liver cirrhosis Grigorieva A.A., Mironova G.E. Prerequisites of ecological safety of animal husbandry products Arctic medicine Mostakhova T.S. Mortality of the population in the northern and arctic regions of the RS (Ya) in the aspect of demographic security Ohlopkova E.D., Olesova L.D., Konstantinova L.I., Mironova G.E., Semenova E.I., Halyev S.D. Physical efficiency and lipid peroxidation in the sportsmen in the Far North Nutrition in the North Kudrina P.I. Nutrition as an adaptation in the living conditions in the North Topical issue Ivanova A.A., Aprosimov L.A., Potapov A.F., Timofeev L.F. Working-age population mortality in the Republic of Sakha (Yakutia): regional characteristics Ivanova O.N., Berezkina O.N. Chronic pancreatitis in children of the RS (Ya) Bessonov P.P., Bessonova N.G., Pestereva V.N., Atlasova L.M., Skrjabina T.V. Analysis of the state of patients after liver transplantation in the RS (Ya) Vinokurova F.V., Golderova A.S., Efremova S.D., Tikhonova O.G., Grigorieva L.V. The cytokine profile in children with juvenile forms of arthritis Scientific reviews and lectures Ushnickij I.D., Nikiforova E.Yu, Ammosova A.M., Cheremkina A.S., Agafonova E. Yu. Modern aspects of the problems of dental diseases in children with connective tissue dysplasia Tikhonov D.G. The pathogenesis of cholelithiasis Sleptsov I.K., Garmaeva D.K The study of the histogenesis of female fetus reproductive system at the mother's urogenital infections Chugunova S.A., Nikolaeva T. Ya. Risk factors for the development and rupture of cerebral aneurysms Point of view Mikhailova A.E., Zakharova R.N., Klimova T.M., Starostina L.D., Timofeeva A.V. The correlation of the subject’s quality of life and clarity of self-concept Clinical case Popova T.E., Tappahov A.A., Nikolaeva T. Ya., Schneider N.A., Petrova M.M., Petrova A. A., Okoneshnikova L.T., Popov D.A. Chronic inflammatory demyelinating polyneuropathy in a patient with Charcot-Marie-Tooth type IA disease Savvina A.D., Egorova T.V., Markova L.O., Mikhailova E.P. Prokhorova L.V., Savvina N.V., Govorova M.D. Health School as a preventive technology Experience exchange Shevchenko A.A., Koshevoj A.V., Kashkarov E.A., Motora V.I., Mjatlik A.V. Surgical correction of chest wall in the treatment of chronic nonspecific pleural empyema with bronchopleural fistula The chronicle of events History pages Nikolaev V.P. To the 150th anniversary of P.N. Sokolnikov Our jubiljars Word about the Master (to the 65th anniversary of the oncology service of Yakutia) B.A. Egorov – 75 years from the date of birth ______________________________ 4’ 2015 6 Kh.A. Kurtanov, A.L. Danilova, A.E. Yakovleva, A.D. Savvina, N.R. Maksimova MOLECULAR AND GENETIC TESTING OF HLA II CLASS GENES IN CELIAC DISEASE PATIENTS IN YAKUTIA ABSTRACT The article presents the results of molecular genetic study based on typing of class II HLA genes (DRB1, DQA1, DQB1) in patients in age from 8 months to 18 years with a referral diagnosis of celiac disease or with suspected celiac disease. The study of the gene HLA class II with three‐ locus haplotypes DRB1‐DQA1‐DQB1 revealed carriers of the haplotypes associated with celiac disease. The researchers found a high frequency of carriers of the haplotypes DRB1*04 ‐ DQA1*03:01 ‐ DQB1*03:02 (DQ8 type) in the Yakuts compared to the Russians. Keywords: celiac disease, DRB1, DQA1, DQB1genes, haplotype. INTRODUCTION Celiac disease is one of the common medical‐social problems of modern gastroenterology. High frequency of complications and development of associated diseases causing the disability testify to necessity of studying this pathology and search of the latest technologies for detecting groups of risk and rehabilitation of patients [1]. Until recently the disease has been observed rarely (1:5000 – 10000). However, nowadays the disease is known to be identified in the majority of countries (from 1:184 to 1:500). According to the researches conducted in Europe and the USA the prevalence of celiac disease among children was 1:80 – 1:300. In Russia no clinical‐epidemiological researches on this pathology have been conducted, its prevalence approximately estimated 1:1000 [1]. Due to a significant role of celiac disease in the etiology of many systematic diseases there is uncertainty of its diagnostics, treatment and rehabilitation that would promote treatment from celiac disease and diseases connected with it, prevention of oncological intestinal diseases [2]. Celiac disease and its complications have multifactorial pathogenesis detected by genetically associated features of metabolism, immune system and gluten hypersensitivity. Of all the theories of celiac disease pathogenesis the genetic theory is considered the most significant ______________________________ 4’ 2015 7 one according to the World Society of Gastroenterologists. The disease progression is connected with genes HLA‐DQ2 (A1*0501 and B1*0201) diagnosed at 90‐95% of patients and HLA DQ‐8 (A1*03 and B1*0302) at 5‐10% of patients [3, 4]. But existence of these genes not always leads to the progression of celiac disease, as non‐HLA genes can be evidence of predictive celiac disease as well [3]. The concept of molecular genetics concerning association of polymorphic genetic markers with predisposition or resistance of the pathology is noted to be one of the approaches of studying genetic risk factors at multifactorial diseases including celiac disease. These markers typical to concrete pathology can be revealed long before its clinical manifestation that will allow to detect risk groups, to organize their monitoring, and if necessary to appoint preventive therapy. Studying of candidate genes with a product of their expression (enzyme, hormone, receptor) involved directly or indirectly in the development of pathological process is of special interest [2]. Due to contemporary achievements in the field of molecular genetics, the study of impact of candidate genes on the disease progression, its clinical manifestations, variability of quantitative signs of immunity and metabolism in the formation of complications appears to be perspective and conductive to the search of risk criterion of celiac disease [1]. In the Republic of Sakha (Yakutia) the prevalence of celiac disease among children in 2008 was 1:1660, and 1:884 among the children's population in Yakutsk city [2]. The absence of unified diagnostic criteria in Yakutia, especially concerning the subclinical forms causing the higher rate of complications and shortcoming of molecular genetics foundations of celiac disease predetermine the expediency of carrying out this research.
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