bioRxiv preprint doi: https://doi.org/10.1101/2020.07.07.191254; this version posted July 7, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Defining the sediment microbiome of the Indian River Lagoon, FL, USA, an Estuary of National 2 Significance 3 4 David J. Bradshaw II,1* Nicholas J. Dickens,1#a John H. Trefry,2 Peter J. McCarthy1 5 6 1Department of Biological Sciences, Harbor Branch Oceanographic Institute at Florida Atlantic 7 University, Fort Pierce, FL, United States of America 8 2Department of Ocean Engineering and Marine Sciences, Florida Institute of Technology, 9 Melbourne, FL, United States of America 10 #aCurrent address: Amazon.com, Inc., London, United Kingdom 11 12 *Corresponding author 13 E-mail:
[email protected] (DJB) 14 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.07.191254; this version posted July 7, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Sediment Microbiome of the Indian River Lagoon… 15 Abstract 16 The Indian River Lagoon, located on the east coast of Florida, USA, is an Estuary of 17 National Significance and an important economic and ecological resource. The Indian River 18 Lagoon faces several environmental pressures, including freshwater discharges through the St.