(12) Patent Application Publication (10) Pub. No.: US 2010/0048405 A1 Raymer Et Al
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US 201000484.05A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2010/0048405 A1 Raymer et al. (43) Pub. Date: Feb. 25, 2010 54) DEVELOPMENT OF fileded on Feb. o.6, 2009 prov1s1onalisional application NNo. HERBCDE-RESISTANT GRASS SPECIES 61/172,427, filed on Apr. 24, 2009. (75) Inventors: Paul L. Raymer, Milner, GA (US); Publication Classification Douglas Heckart, Athens, GA (US); Wayne Allen Parrott, (51) Int. Cl. Athens, GA (US) AON 3.5/10 (2006.01) s AOIH 5/00 (2006.01) Correspondence Address: AOIH I/06 (2006.01) DAVIS WRIGHT TREMAINE LLP - San Fran- CI2O 1/02 (2006.01) cisco CI2N 5/04 (2006.01) 505 MONTGOMERYSTREET, SUITE 800 C7H 2L/00 (2006.01) SAN FRANCISCO, CA 94111 (US) AOIPI3/00 (2006.01) rsr rr (52) U.S. Cl. ......... 504/343; 800/300; 800/267: 800/265; (73) Assignee: ENEEOundation, o'ssor enS, Rath 800/264; 800/263; 435/29: 435/410:536/23.1 (21) Appl. No.: 12/488,452 (57) ABSTRACT Thee i1nVent1On relates1 to a Selectedlected and culturedCulture ACC a SC (22) Filed: Jun. 19, 2009 inhibitor herbicide-resistant plant-resistant plant from the group Panicodae, or tissue, seed, or progeny thereof, and Related U.S. Application Data methods of selecting the same. The invention also relates to (60) Provisional application No. 61/074.381, filed on Jun. methods for controlling weeds in the vicinity of an ACCase 20, 2008, provisional application No. 61/150,459, inhibitor herbicide-resistant plant. FATY ACD BIOSYNTHESIS FATY ACDS Patent Application Publication Feb. 25, 2010 Sheet 1 of 16 US 2010/00484.05 A1 SISEHINUS018GiovAuxº yoº-TUJEOY SCIË,DYALIA, I"OIH ºoo}}~~^?wByuzNGOOTAIBOw !3SYTAXOBAIYO Patent Application Publication Feb. 25, 2010 Sheet 2 of 16 US 2010/00484.05 A1 Patent Application Publication Feb. 25, 2010 Sheet 3 of 16 US 2010/0048405 A1 1600 14 OO 1200 1 OOO 8OO 6OO 400 2OO -200 uM Sethoxydim F.G. 3 Patent Application Publication Feb. 25, 2010 Sheet 4 of 16 US 2010/00484.05 A1 §§§ Patent Application Publication Feb. 25, 2010 Sheet S of 16 US 2010/00484.05 A1 | S"?INH Patent Application Publication Feb. 25, 2010 Sheet 6 of 16 US 2010/00484.05 A1 Patent Application Publication Feb. 25, 2010 Sheet 7 of 16 US 2010/0048405 A1 Sethoxydim injury (d. 7 DAT ammu jaira Kaaret urges line A i teles issue Cuit Control f s fif t 3. 3i: sethoxyid trn rate grams atha FIG. 7 Patent Application Publication Feb. 25, 2010 Sheet 8 of 16 US 2010/00484.05 A1 Patent Application Publication Feb. 25, 2010 Sheet 9 of 16 US 2010/00484.05 A1 Sethoxydim injury G) 14DAT Amelii is a gi issie C. Cit : sees line A SC 3. 35 Seixytim Rate grants aifa F.G. 9 Patent Application Publication Feb. 25, 2010 Sheet 10 of 16 US 2010/00484.05 A1 Patent Application Publication Feb. 25, 2010 Sheet 11 of 16 US 2010/00484.05 A1 Sethoxydim injury GD 21 DAT anewatira Kea are it Tissue Culture Control resee Line A s S. 5. Sethgxydim Rate gras alia) F.G. Patent Application Publication Feb. 25, 2010 Sheet 12 of 16 US 2010/00484.05 A1 O 5OO 1OOO 15OO 2OOO 25OO 3000 3500 Sethoxydim Rate (grams ai. per hectare) F.G. 12 Patent Application Publication Feb. 25, 2010 Sheet 13 of 16 US 2010/0048405 A1 s : --TCC N 50 no-on-AF--- or re-or--- - - A - Line A m mXie LineL. B - E. --- -------- - - - - - - - - -n O 1000 2000 3000 4000 5000 6000 7000 Sethoxydim Rate (grams a. i. per hectare) F.G. 13 Patent Application Publication Feb. 25, 2010 Sheet 14 of 16 US 2010/00484.05 A1 100 O 500 1000 1500 2000 2500 300 3500 Fluazifop-p-butyl Rate (grams a. i. per hectare) F.G. 14 Patent Application Publication Feb. 25, 2010 Sheet 15 of 16 US 2010/00484.05 A1 area -- TCC s S. 60 --—------------ to a Line A -------W----- s axa lines – N 50 | S 40 -WW-W,-------------------- E . A s ai 30 ----- S-M-WW es e a. 20 - e-------X---- re----------- --W O 500 OOO 1500 2000 2500 3000 3500 Fenoxaprop-p-ethyl Rate (grams a. i. per hectare) FG 15 Patent Application Publication Feb. 25, 2010 Sheet 16 of 16 US 2010/00484.05 A1 F.G. 6 US 2010/0048405 A1 Feb. 25, 2010 DEVELOPMENT OF an amount Sufficient to retard growth or kill the callus, select HERBCDE-RESISTANT GRASS SPECIES ing at least one resistant cell based upon a differential effect of the herbicide, and regenerating a viable whole plant of the CROSS-REFERENCE TO RELATED variety from the at least one resistant cell. In some embodi APPLICATIONS ments, the plant is a non-transgenic plant. 0001. This application claims priority from U.S. Provi 0006. In some embodiments of the invention, the ACCase sional Application Ser. No. 61/074.381, filed on Jun. 20. inhibitor herbicide-resistant plant is a member of tribe Pan 2008, U.S. Provisional Application Ser. No. 61/150,459, filed iceae. In some embodiments, the ACCase inhibitor herbicide on Feb. 6, 2009, and U.S. Provisional Application Ser. No. resistant plant is one selected from the group of Axonopus 61/172,427, filed on Apr. 24, 2009, each of which is incorpo (carpetgrass), Digiteria (crabgrass), Echinochloa, Panicum, rated herein by reference in its entirety. Paspalum (Bahiagrass), Pennisetum, Setaria and Steno taphrum (St. Augustine grass). In some embodiments, the FIELD ACCase inhibitor herbicide-resistant plant is one selected from the group of seashore paspalum (P. vaginatum), bent 0002 The invention disclosed herein generally relates to grass, tall fescue grass, Zoysiagrass, bermudagrass (Cynodon grasses with resistance to selective grass herbicides and meth spp), Kentucky Bluegrass, Texas Bluegrass, Perennial ods to develop the same. ryegrass, buffalograss (Buchloe dactyloides), centipedegrass (Eremochloa Ophiuroides) and St. Augustine grass (Steno BACKGROUND taphrum secundatum), Carpetgrass (Axonopus spp.) and 0003 Seashore paspalum (Paspalum vaginatum) is a Bahiagrass (Paspalum notatum). warm-season turfgrass that is generally adapted to dune envi 0007. In some embodiments of the invention, the ACCase ronments. Favorable attributes of seashore paspalum include inhibitor herbicide-resistant plant is resistant to an acetyl its tolerance to salt, water logging, and drought. These char coenzyme A carboxylase (ACCase) inhibitor. In some acteristics make paspalum a premium turfgrass candidate for embodiments, the ACCase inhibitor herbicide-resistant plant venues where any or all of these environmental problems is resistant to a cyclohexanedione herbicide, an aryloxyphe could be an issue. For example, golf course architects recom noxy proprionate herbicide, a phenylpyrazoline herbicide, or mend seashore paspalum for new courses in tropical or Sub mixtures thereof. In some embodiments, the ACCase inhibi tropical coastal areas where salt or water quality can affect tor herbicide-resistant plant is resistant to at least one herbi turfgrass growth and maintenance. In addition, many existing cide selected from the group of alloxydim, butroxydim, clo golf courses have replaced bermudagrass (Cynodon dacty proxydim, profoxydim, Sethoxydim, clefoxydim, clethodim, lon) with paspalum. Compared to bermudagrass, paspalum cycloxydim, tepraloxydim, tralkoxydim, chloraizfop, clodin requires less nitrogen and is more tolerant of irrigation with afop, clofop, cyhalofop, diclofop, fenoxaprop, fenthiaprop, brackish or poor quality water, which reduces management fluaZafop-butyl, fluazifop, haloxyflop, isoxapyrifop, metami costs and improves irrigation flexibility. fop, propaquizafop, quizalofop, trifop and pinoxaden. 0004. A main limitation to replacing bermudagrass with 0008. In some embodiments of the invention, the herbi paspalum is bermudagrass re-establishment. Bermudagrass cide resistance of the ACCase inhibitor herbicide-resistant is highly competitive and difficult to eradicate once estab plant is conferred by a mutation at least one amino acid lished. Bermudagrass and other weedy grasses can greatly position of ACCase gene selected from the group of 1756, reduce the aesthetic value and quality of the paspalum turf. 1781, 1999, 2027, 2041, 2078, 2099 and 2096. In some Accordingly, it is desired to control or limit bermudagrass or embodiments, the herbicide resistance is conferred by an weedy grass growth in paspalum-populated areas. To control isoleucine to leucine mutation at amino acid position 1781. the growth of weedy grasses in paspalum-populated turfgrass 0009 Embodiments of the invention also relate to a prog areas, the development of paspalum turfgrass with resistance eny of an ACCase inhibitor herbicide-resistant plant plant as to selective grass herbicides is desired. Past approaches in described in any of the foregoing paragraphs. In some development of herbicide-resistant turfgrass include the use embodiments, the progeny is a result of sexual reproduction of genetic engineering approaches. However, plants pro of the ACCase inhibitor herbicide-resistant plant parent. In duced by genetic engineering approaches may be difficult to Some embodiments, the progeny is a result of asexual repro commercialize due to governmental regulations and restric duction of the ACCase inhibitor herbicide-resistant plant par tions regarding the use of genetically modified plants. ent Accordingly, embodiments of the invention include the 0010 Embodiments of the invention are also directed to a development of turfgrass cultivars with non-transgenic resis seed of an ACCase inhibitor herbicide-resistant plant as tance to herbicides, as well as cultivars with transgenic resis described in any of the foregoing paragraphs, or a progeny tance. thereof. 0011 Embodiments of the invention relate to sod compris SUMMARY ing an ACCase inhibitor herbicide-resistant plant of as 0005 Embodiments of the invention relate to a selected described in any of the foregoing paragraphs, or a progeny or and cultured ACCase inhibitor herbicide-resistant plant-re seed thereof. Embodiments of the invention are also directed sistant plant from the group Panicodae, or tissue, seed, or a turfgrass nursery plot comprising an ACCase inhibitor her progeny thereof.