Molecular Biogeography of Grasses and Tropical Grasslands Jan Hackel
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Molecular biogeography of grasses and tropical grasslands Jan Hackel To cite this version: Jan Hackel. Molecular biogeography of grasses and tropical grasslands. Vegetal Biology. Université Paul Sabatier - Toulouse III, 2017. English. NNT : 2017TOU30222. tel-03123970 HAL Id: tel-03123970 https://tel.archives-ouvertes.fr/tel-03123970 Submitted on 28 Jan 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Molecular biogeography of grasses and tropical grasslands Biogéographie moléculaire des graminées et des savanes tropicales Jan Hackel 13 December 2017 Doctoral dissertation Thèse de doctorat Université Toulouse III – Paul Sabatier Laboratoire Evolution et Diversité Biologique Supervisor/Directeur de thèse: Guillaume Besnard Examination board/Jury de thèse: Monique Gardes, Université Toulouse III – Paul Sabatier Alex Baumel, Aix-Marseille Université (rapporteur) Peter Linder, Universität Zürich Yves Vigouroux, IRD Montpellier (rapporteur) Acknowledgements I would like to thank a number of people for accompanying me through these last three years. First of all, Guillaume Besnard was a great supervisor, always available for feedback, with this intuition for the curious details, and we spent hours in the afternoon heat of Madagascar sterilising grass leaves. Maria Vorontsova was involved in all parts of this dissertation. Pilar Catalán gave me the opportunity to participate in her and Miguel Minaya's work on Loliinae. Colleagues at EDB made these three years a great time scientifically and personally. Sophie Manzi and Amaia Iribar-Pelozuelo helped me with lab work issues. Hervé Gryta was member of my PhD committee and had useful suggestions on several occasions. Lucie Zinger discussed metabarcoding and helped finding those little errors in my scripts. Patricia Jargeat provided fungal isolates. Christophe Thébaud lent me useful books on Madagascar. Antoine Fouquet critically read an article manuscript. The lab administration and the doctoral school made everything run smoothly. My office colleagues, fellow PhD students, and all those who went running, swimming or hiking with me helped me stay balanced and have a good time. Alexandre Meunier was a fast-learning MSc intern. And not to forget all the students who had to endure my first steps in teaching! Several people outside Toulouse gave valuable input. P-A Christin, member of my PhD committee, was always available for questions. Matheus Bianconi shared great results of his parallel analyses on Jansenella and Co. Corinne Vacher, also member of my PhD committee, shared her expertise on the leaf microbiome. Several people answered questions or even shared data, including Elizabeth Kellogg, Caroline Strömberg and Jana U'Ren. Comments of anonymous reviewers greatly improved my papers. I also thank the countless contributors of open software, tutorials, Wikipedia articles and other free content on the internet; I believe they make science better and they certainly made my life easier. Finally, I thank Alex Baumel, Yves Vigouroux and Peter Linder for agreeing to examine my dissertation. The 2016 trip to Madagascar was an incredible experience and I would like to thank all people involved. Special mention to Hélène Ralimanana, Jacqueline Razanatsoa, Cédrique Solofonanatra, Herison Andrianirina and Caroline Lehmann (and of course Maria), and the whole of the Kew Madagascar Conservation Centre in Antananarivo. I hope my little editing on the Flora of Madagascar on Wikipedia, facilitated by KMCC's library, is a small contribution to their work on plant documentation and conservation. This dissertation was made possible through a contract funded by the French Government and through other public funds, including from two “Labex” (CEBA, ANR-10-LABX-25-01 and TULIP, ANR-10-LABX-0041) and an Idex UNITI student mobility grant for fieldwork in Madagascar (RECAC15EX110). The last two years in Europe and the world reminded me that the freedom of students and academics to move and exchange is far from a given and I am grateful for the opportunity to study and work in France. My final thanks go to all my loved ones who supported me. Danke, merci! Table of contents 1 Introduction ...................................................................................................................4 2 Chapter I: Factors underlying the global dispersal of temperate Loliinae grasses ...22 3 Chapter II: Grass diversification in Madagascar .......................................................32 4 Chapter III: Phylogenomics and biogeography of the core Panicoideae ...................49 5 Chapter IV: A metabarcoding method for the analysis of grass endophyte communities in Madagascar ..................................................................................68 6 Conclusion and perspectives .......................................................................................83 7 Annex ...........................................................................................................................90 Introduction 1 Introduction 1.1 Patterns and processes in biogeography The spatial distribution of species Understanding the distribution of organisms on Earth has been a long-standing goal of biology. Why are some regions more diverse than others? Why are some species endemic and others widespread? How have groups with a common ancestor come to colonise widely separated ranges? Questions such as these form the core of the discipline of biogeography (Cox et al. 2016). Studying the geography of earth's biodiversity is an challenging area of fundamental research but has implications that go beyond pure scientific interest. Organisms interact with their physical environment and each other, forming dynamic ecosystems (Tansley 1935) which not least are the basis of human civilisation. In an epoch where humans are modifying the biosphere in unprecedented ways, both through physical alteration and the rearrangement of biotic communities (Waters et al. 2016), it is urgent to understand the fundamental processes behind the spatial distribution of biodiversity. Biodiversity can be studied at various spatial and temporal scales, from biomes to genes, and from geological epochs to days, and these levels all have their own dynamics and influence each other (Levin 1992). One of the most commonly studied units, both in evolutionary biology and ecology, is the species. The existence of species appears an evident fact to most, but defining and delimiting species has been a major challenge in biology. Today, most biologists explicitly or implicitly share a species concept rooted in the theory of evolution, although they may be divided on how to detect and tell apart species in practice (Hey 2006, de Queiroz 2007). A species is seen as an evolutionary independent lineage of organisms that has split from another lineage, a “separately evolving metapopulation lineage” (de Queiroz 2007). This is the concept I also apply in this dissertation. The distribution of species on the planet is highly uneven, but some generalities do exist. For example, most species in a given locality are rare (Preston 1948), the number of species increases with area in a repeatable manner (McGuinness 1984), and, on a global scale, the tropics have more species than higher latitudes in most groups of organisms (Hillebrand 2004). However, beneath these general observations, there is a diversity of patterns more complicated: the taxonomic composition of species assemblages differs between continents, creating distinct biogeographic regions recognised since more than a century (Sclater 1858, Engler 1879). Physically similar biomes, such as tropical forests, savannahs or high mountains, can be found on different continents, but the species, the biota, that compose them differ (Nelson 1978). Biogeography has traditionally addressed these patterns from two angles: an ecological one, focusing on the current-day, observable mechanisms that shape distribution patterns, and a historical–evolutionary one, studying the impact of past geological events and evolutionary history (Cox et al. 2016). These two have long existed as two parallel 4 Introduction disciplines, with little exchange, but there have been increasing efforts to merge them (Wiens & Donoghue 2004, Ricklefs & Jenkins 2011). Ecological processes Thinking in ecology has been strongly influenced by the idea that an organism is adapted to a particular set of environmental factors and other organisms it interacts with, which together form its niche (Grinnell 1917, Elton 1927, Hutchinson 1957). Thus, the niche of an organism would determine where it occurs, and the environment would “filter” species adapted to it (Weiher et al. 2011). This is evident in many cases, with e.g. plant species having adaptations to cold, arid, or humid climates, and being unable to survive under opposing conditions. Species interact with each other, and in a given location, different organisms with exactly the same niches are theoretically expected to exclude each other (Hardin 1960). Organisms may however co-exist when they