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Somatic Embryogenesis and Regeneration of Endangered Cycad Species
Somatic Embryogenesis and Regeneration of Endangered Cycad Species R.E. Litz and P.A. Moon V.M. Chavez Avila Tropical Research and Education Center Jardin Botanico, Instituto de Biologia University of Florida Universidad Nacional Autonoma de Mexico 18905 SW 280 Street Apartado Postal 70-614 Homestead FL, 33031-3314 04510 Mexico DF USA Mexico Keywords: Somatic embryo, gymnosperm, Cycadales, conservation Abstract The Cycadales (Gymnospermae) include some of the world's most endangered and rare plant species. Many of the cycad species are known only as single specimen trees (e.g., Encephalartos woodii), as very small populations in the wild (e.g., Ceratozamia hildae) or have become extinct in the wild (e.g., Ceratozamia euryphyllidia). All cycads are dioecious, so that seed production is no longer possible with the rarest of the species. Conditions for induction of embryogenic cultures from leaves of mature phase trees of several species in the family Zamiaceae have been reported, and plants have been regenerated from somatic embryos. Embryogenic cultures of two species have been successfully cryopreserved. These strategies should contribute to the conservation of these endangered species and could lay the basis for commercial propagation of these beautiful but rare plants. INTRODUCTION The Cycadales represent the most ancient surviving group of higher plants, having arisen during the Permian era and flourished in the Mesozoic and Jurassic periods. They have been referred to as "living fossils" (Gilbert, 1984). Norstog (1987) considered that the cycads are unique for the study of the evolution of development in higher plants. There are only three extant cycad families, the Cycadaceae, Stangeriaceae and Zamiaceae, and these contain approximately 224 species. -
Lithops and Lithops Turbiniformis (Haw.) N.E.Brown As of 19 September 2014
A Brief History of the Genus Lithops and Lithops turbiniformis (Haw.) N.E.Brown As of 19 September 2014 The genus Lithops is part of the family Aizoaceae and of the subfamily Ruschioideae, one of 5 such subfamilies of the family Aizoaceae. The genus name Lithops was first described by Nicholas Edward Brown (1849-1934) in 1922. He was a herbarium botanist and taxonomist in England. The Lithops name comes from the Greek lithos which means 'stone' and óps which means 'appearance' or 'a face'. They look like 'Living Stones', a common name we like to use for them. (The word Lithops is used as both singular and plural form.) Each Lithops has one pair of leaves with a fissure in between where a solitary flower is produced. In habitat the tops of the leaves are either at ground level often wedged between stones or slightly buried, especially during a dry period. The tops of the leaves appear to be either flat or somewhat raised and more or less rough looking as if cut off short with a translucent window or window-like spots. The size across the two leaves at the apex is generally ¾ to 1½" in diameter. It's a mimicry plant in habitat with surrounding stones of similar size and shape until it flowers. The Flower color varies from yellow to white to bronze to pink. The natural habitat of Lithops is in the dry regions of southern Africa—from the Cape and Transvaal Province regions of the Republic of South Africa into much of the western coast to central and southern parts of Namibia. -
Exposing the Illegal Trade in Cycad Species (Cycadophyta: Encephalartos) at Two Traditional Medicine Markets in South Africa Using DNA Barcoding1 J
771 ARTICLE Exposing the illegal trade in cycad species (Cycadophyta: Encephalartos) at two traditional medicine markets in South Africa using DNA barcoding1 J. Williamson, O. Maurin, S.N.S. Shiba, H. van der Bank, M. Pfab, M. Pilusa, R.M. Kabongo, and M. van der Bank Abstract: Species in the cycad genus Encephalartos are listed in CITES Appendix I and as Threatened or Protected Species in terms of South Africa’s National Environmental Management: Biodiversity Act (NEM:BA) of 2004. Despite regulations, illegal plant harvesting for medicinal trade has continued in South Africa and resulted in declines in cycad populations and even complete loss of sub-populations. Encephalartos is traded at traditional medicine markets in South Africa in the form of bark strips and stem sections; thus, determining the species traded presents a major challenge due to a lack of characteristic plant parts. Here, a case study is presented on the use of DNA barcoding to identify cycads sold at the Faraday and Warwick traditional medicine markets in Johannesburg and Durban, respectively. Market samples were sequenced for the core DNA barcodes (rbcLa and matK) as well as two additional regions: nrITS and trnH-psbA. The barcoding database for cycads at the University of Johannesburg was utilized to assign query samples to known species. Three approaches were followed: tree-based, similarity-based, and character-based (BRONX) methods. Market sam- ples identified were Encephalartos ferox (Near Threatened), Encephalartos lebomboensis (Endangered), Encephalartos natalensis (Near Threatened), Encephalartos senticosus (Vulnerable), and Encephalartos villosus (Least Concern). Results from this study are crucial for making appropriate assessments and decisions on how to manage these markets. -
The International Cultivar Registration Authority Register and Checklist for the Genus Lithops N.E.Br
The International Cultivar Registration Authority Register and Checklist for the genus Lithops N.E.Br. by Keith Green The list that follows is the official checklist and register of Lithops cultivars, in alphabetical order according to cultivar epithet. It was first compiled as a checklist in the (UK) autumn of 2013, but subsequent to that date it is requested new cultivars be registered using the official form available from the link on the right. As it is impossible to monitor every publication worldwide, it is the responsibility of anyone proposing a new Lithops cultivar to alert the registrar. Where the term “First published” is used below, it is to the best belief of the registrar. At a botanic level the list follows the Cole classification system. NB. The term “Nomenclatural standard” is used only where a photograph has so been designated; the term “Image example” being used elsewhere. No offence is inferred by the omission or use of personal titles (such as Mr., Mrs., Dr., etc.) which are recorded as researched by, or presented to the registrar. Names attributed to Lithops as personal or casual notations or in erroneous format may not appear. Entries in red, preceded by an asterisk * are unacceptable cultivar epithets. ‘Akahada Reikogyoku’ Lithops dorotheae ‘Akahada Reikogyoku’. First published by Norihiko Shimada in “Cultivars from Shimada’s Kitchen, Kaktusy, LV11 Special (2): 30-50. 2021”. Image example: Fig. 5, accompanying the protologue taken by Norihiko Shimada of Japan. A red flushed L. dorotheae. 'Albiflora' Lithops lesliei subsp. lesliei var. lesliei 'Albiflora'. This was published as a cultivar by Professor Desmond Cole in “Some Lithops Cultivars, Aloe 22(3): 58-62. -
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ISSN 2473-442X CONTENTS Message from Dr. Patrick Griffith, Co-chair, IUCN/SSC CSG 3 Official newsletter of IUCN/SSC Cycad Specialist Group Botanic Garden: In Focus Vol. IV I Issue 2 I December 2019 Montgomery Botanical Center’s Cycad Collection – Focus on research and conservation 5 Michael Calonje & Patrick Griffith Feature Articles Towards an approach for the conservation and illegal trade prevention of South Africa’s endangered Encephalartos spp. 10 James A. R. Clugston, Michelle Van Der Bankand Ronny M. Kobongo Fire is the most important threat for conservation of Dioon merolae (espadaña) in the hill Nambiyigua, municipality of Villaflores, Chiapas, Mexico 13 Miguel Angel Pérez-Farrera & Mauricio Martínez Martínez Ex-situ Cycad Conservation [1]: Public and Private Collections 16 Chip Jones & JS Khuraijam The Cycad Specialist Group (CSG) is a component of the IUCN Species Research and Conservation News Survival Commission (IUCN/SSC). It consists of a group of volunteer The Cycad Extinction Crisis in South Africa 19 experts addressing conservation Wynand van Eeden & Tim Gregory issues related to cycads, a highly What is Ceratozamia becerrae ? 21 threatened group of land plants. The Andrew P. Vovides, Miguel Angel Pérez-Farrera & José Said Gutiérrez-Ortega CSG exists to bring together the world’s cycad conservation expertise, Preliminary Finding: Seed longevity of Encephalartos in controlled storage 23 and to disseminate this expertise to Ngawethu Ngaka and Phakamani Xaba organizations and agencies which can use this guidance to advance cycad Meeting Reports conservation. 2nd Nong Nooch Cycad Horticulture Workshop 25 Official website of CSG: Anders Lindstrom http://www.cycadgroup.org/ Plant Conservation Genetics Workshop 26 Co-Chairs Caroline Iacuaniello, Stephanie Steele & Christy Powell John Donaldson Patrick Griffith CSG Members 28 Vice Chairs Michael Calonje Cristina Lopez-Gallego Red List Authority Coordinator De Wet Bosenberg CSG Newsletter Committee JS Khuraijam, Editor Irene Terry Andrew P. -
Encephalartos Woodii ELSA POOLEY Writes About the Mysterious Enigma Wood’S Cycad, a Plant That Is Extinct in the Wild
Encephalartos woodii ELSA POOLEY writes about the mysterious enigma Wood’s cycad, a plant that is extinct in the wild. Wood’s cycad is one of the most magnificent and rare plants of this family of ancient plants. It has been extinct in the wild for nearly a century. Only one four-stemmed male plant was ever found. It was first collected by John Medley Wood, director of the Natal Government Herbarium and leading Natal botanist. He was on a botanical expedition in Zululand in 1895, and found it when exploring Ngoye Forest (now spelt Ongoye). In 1903 several suckers Encephalartos woodii growing in Durban Botanic Gardens. photograph © Richard Boon 60 INTERNATIONAL DENDROLOGY SOCIETY TREES were collected for the Durban Botanic Gardens and for the Royal Botanic Gardens at Kew and a private nurseryman in the south of England. (It was described in the Gardeners’ Chronicle in 1908.) In 1907 John Wylie, an assistant to Medley Wood and curator of the Durban Botanic Gardens, collected two of the large trunks and planted them in the Durban Botanic Garden. In 1916 the last surviving stem was removed and was planted in Pretoria. All efforts to locate more plants – and female plants – in the original location have failed. However, hundreds of offshoots have been grown and distributed around the world, so the plant is known outside of South Africa. This is a distinctive species. The original plants stand about 6m tall, with a stately, erect stem which is broad and buttressed at the base. This buttressed stem, and the spreading canopy of arching leaves, even in juvenile plants, distinguishes the species. -
Crime, Culture and Collecting: the Illicit Cycad Market in South Africa
CRIME, CULTURE AND COLLECTING: THE ILLICIT CYCAD MARKET IN SOUTH AFRICA BY JONAS SØRFLATEN TORGERSEN SUPERVISED BY Town PROFESSOR MARK SHAW Cape of THESIS Submitted in partial fulfilment of the requirements for the degree of Master of Philosophy in University Criminology, Law and Society in the Faculty of Law at the University of Cape Town, 2017 Cape Town, South Africa The copyright of this thesis vests in the author. No quotation from it or information derived from it is to be published without full acknowledgement of the source. The thesis is to be used for private study or non- commercial research purposes only. Published by the University of Cape Town (UCT) in terms of the non-exclusive license granted to UCT by the author. University of Cape Town CRIME, CULTURE AND COLLECTING: THE ILLICIT CYCAD MARKET IN SOUTH AFRICA Abstract It is widely accepted that illicit markets are driven by specific contextual factors that determine their nature and scope. Two points in particular have not been explored in the literature on wildlife crime. First, while illicit markets around commodities such as drugs and weapons are fuelled by consumers arguably in need of, or addicted to, the product, the desires of buyers that shape wildlife markets are often shaped by cultural norms which may seem irrational to outsiders. Second, given that wildlife markets are seldom as stringently regulated as those in respect of drugs, weapons or other commodities, the nature of the criminal enterprises that source, move and sell the products are possibly very different. The study examines these two factors – the culture of markets and the degree of criminal enterprise or organisation within them – through a case study of a largely unexamined environmental crime market in South Africa, that of rare cycad plants. -
Inducing Sex Change and Organogenesis from Tissue Culture
114 SouthAfrican Journal of Science98, March/April2002 Gommentary reveals slight heterochromatin differ- lnducing sex change and ences,"'" which is itself due to differential methylation."''n Therefore, it is distinctly organogenesis from tissue possible that methylation controls sex determination. culture in the endangered African Methylation and accompanying hetero- chromatin can be removed by various cycad Encephalartos woodii f actors - such as temper attJte,'5''6 lighft ,27 osmotic stress,28or hormones2e-31- result- (Gycadales, Zamiaceael ing in sex change.3''33Sex change occurs only in organisms that have (virtually) indistinguishable sex chromosomes, indi- Root Gorelicku" and Roy Osborneb cating that incipient sex chromosomes are formed by slight differences in methylation. Differential methylation is I F INCIPIENT SEX CHROMOSOME DIFFEREN- Fourth, if backcrossing is the only realistic evolutionarily the first difference between I tiutiott is caused by differential methy- approach to conservation, then it is pref- females and males3nand is the likely cause I lation between females and males, then erableto use E.woodiiasthe female parent of reported sex changes in cycads. methylating or demethylating cytosine because of maternal inheritance of nucleotides may induce sex change. chloroplast genomes. Fifth, induced sex Methylation may also stimulate regeneration Application of theory to sex change of roots and shoots from tissue culture callus change may assist in the conservation of in cycads and increase genetic variation via greater -
Lithops Scrapbook: Part 1’, Comment on ‘Data on Lithops Cultivar Names’, Cactus World, Formosa, V
Painting of L. julii subsp. fulleri var. brunnea © Jim Porter and reproduced with kind permission. Brief additional notes to the Cole Lithops monographs by Keith Green. Introduction An abridged version of these notes was published over three issues by the BRITISH CACTUS AND SUCCULENT SOCIETY in their journal CACTUS WORLD, in December 2007, March 2008 and June 2008. This is the complete, unedited project. The following notes evolved from my intention to provide an update (without any duplication) to Professor DESMOND T. COLE’s original Lithops monograph - LITHOPS FLOWERING STONES, published in Randburg, Republic of South Africa by Acorn Books in 1988. An attempt was made to briefly document all of the subsequent discoveries within the genus, with emphasis on the originating source. I gave consideration to every “new” Lithops I saw mentioned (the vast majority of which were termed cultivars) and documented, further researched and where possible obtained photographs of those I considered worthy of the rank afforded them. Over the years I therefore amassed quite a reasonable number of entries. Early in 2003 I learned through the pages of the M.S.G. Bulletin that Professor Cole was going to update his work and have a second edition Lithops monograph published. Subsequently I was able to make contact with Professor Cole, and I sent him a rough copy of these (then embryonic) notes hoping that they would be of some assistance to him in compiling his new book. Although he and Naureen kindly mention my help on p. 11 of ‘Cole’05’, I learnt a great deal more from the Coles’ than they could ever have learnt from me! Professor Cole’s reply (which included some Lithops seed) was most informative. -
Red Data List Special Edition
Newsletter of the Southern African Botanical Diversity Network Volume 6 No. 3 ISSN 1027-4286 November 2001 Invasive Alien Plants Part 2 Southern Mozambique Expedition Living Plant Collections: Lowveld, Mozambique, Namibia REDSABONET NewsDATA Vol. 6 No. 3 November LIST 2001 SPECIAL EDITION153 c o n t e n t s Red Data List Features Special 157 Profile: Ezekeil Kwembeya ON OUR COVER: 158 Profile: Anthony Mapaura Ferraria schaeferi, a vulnerable 162 Red Data Lists in Southern Namibian near-endemic. 159 Tribute to Paseka Mafa (Photo: G. Owen-Smith) Africa: Past, Present, and Future 190 Proceedings of the GTI Cover Stories 169 Plant Red Data Books and Africa Regional Workshop the National Botanical 195 Herbarium Managers’ 162 Red Data List Special Institute Course 192 Invasive Alien Plants in 170 Mozambique RDL 199 11th SSC Workshop Southern Africa 209 Further Notes on South 196 Announcing the Southern 173 Gauteng Red Data Plant Africa’s Brachystegia Mozambique Expedition Policy spiciformis 202 Living Plant Collections: 175 Swaziland Flora Protection 212 African Botanic Gardens Mozambique Bill Congress for 2002 204 Living Plant Collections: 176 Lesotho’s State of 214 Index Herbariorum Update Namibia Environment Report 206 Living Plant Collections: 178 Marine Fishes: Are IUCN Lowveld, South Africa Red List Criteria Adequate? Book Reviews 179 Evaluating Data Deficient Taxa Against IUCN 223 Flowering Plants of the Criterion B Kalahari Dunes 180 Charcoal Production in 224 Water Plants of Namibia Malawi 225 Trees and Shrubs of the 183 Threatened -
FOR IMMEDIATE RELEASE: January 21, 2016
FOR IMMEDIATE RELEASE: January 21, 2016 NYBG’s Wild Medicine in the Tropics Explores the Healing Power of Plants and Offers Relief from Winter’s Icy Grip in the Warmth of the Enid A. Haupt Conservatory Running January 23–February 21, 2016, Exhibition Highlights Include Special Valentine’s Day Weekend Events and New Spanish-Language Signage and Mobile Guide The Lowland Tropical Rain Forest Gallery in the Enid A. Haupt Conservatory is home to many of the medicinal plants featured in Wild Medicine in the Tropics. Bronx, NY— After a mild start, winter has returned to the New York area in full force. There’s no better time to explore the warm rain forest and desert galleries of the landmark Enid A. Haupt Conservatory at The New York Botanical Garden, where visitors can discover how plants are used for health and wellness during Wild Medicine in the Tropics. The Botanical Garden’s winter exhibition, which opens January 23 and runs through February 21, 2016, draws on the Haupt Conservatory’s richly varied permanent collection of tropical trees, exotic flowers, and desert succulents to underscore the essential role that plants play in human health—and provides a welcome escape from winter’s icy grip. With interpretative signage, an award-winning iPhone app, and related programming for adults and children, Wild Medicine in the Tropics shows how cultures around the world rely on plants for everything from medicine to cosmetics and features plants that are essential to a wide variety of healing traditions. Learn more about NYBG’s anniversary at nybg.org/125 Continuing the bilingual signage that the Garden used for last year’s highly acclaimed exhibition Frida Kahlo: Art, Garden, Life, many of the signs for Wild Medicine in the Tropics will be in Spanish as well as English, and a Spanish-language guide to the exhibition will be available on the mobile version of the Garden’s Web site. -
GREEN GARDENS AZORES PROJECT: AZORES ABRIEF GARDENS GREEN in the AZORES’ HISTORICAL GARDENS HISTORICAL AZORES’ the in : Azores, Flora De Jardín, Turismo De Jardines
GREEN GARDENS AZORES PROJECT: A BRIEF CHARACTERIZATION OF THE VASCULAR FLORA IN THE AZORES’ HISTORICAL GARDENS Maria João Pereira*, Raimundo Quintal**, Carina Costa*** & Isabel Albergaria**** Abstract The Green Gardens Azores Project is part of the action plan for tourism development in Portugal (2014-2020) aiming to integrate the Azorean Gardens in the circuit of international ‘Garden Tourism’. With that purpose we built a checklist of the vascular plants cultivated in 8 Azorean Historical Gardens. The analysis of this checklist reveals a richness of 1884 specific and infra-specific taxa, hybrids and cultivars. This richness is represented by 168 families, 514 genera, 991 species, 288 hybrids and 958 cultivars. Camellia hybrids correspond to 60% of all the hybrids and Camellia cultivars represent 71% of all the cultivars. Zamiaceae is the family best represented with 73 species while the best represented genera are Encephalartos with 48 species and Camellia with 45 species. The presence of 5 species extinct in the wild and 96 threatened species in the Azorean Gardens stresses the role of the Gardens in the Conservation of World Flora. Keywords: Azores, Garden flora, Garden tourism. EL PROYECTO «JARDINES VERDES DE AZORES»: BREVE CARACTERIZACIÓN DE LA FLORA VASCULAR DE LOS JARDINES HISTÓRICOS DE AZORES 11 Resumen El proyecto «jardines verdes de Azores» es parte de un plan de acción para el desarrollo turístico de Portugal (2014-2020) pretendiendo integrar estos jardines en el circuito inter- nacional de jardines turísticos. Con este propósito hicimos la redacción de un listado de plantas vasculares cultivadas en ocho jardines históricos de Azores. El análisis del listado señaló una riqueza de 1884 categorías específicas, infraespecíficas, híbridos y cultivares.