Hepatic Lipidomics Reveals the Homeostasis and Prole of Sphingomyelin from Yak Butter in Normal- Fat Diet-Fed Rats Xin LUO Qinghai University Wancheng SUN Qinghai University Yihao LUO (
[email protected] ) Qinghai University Research Keywords: sphingomyelin, lipidomics, steatosis, inammation, lipid metabolism Posted Date: December 3rd, 2020 DOI: https://doi.org/10.21203/rs.3.rs-118802/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License 1 Hepatic Lipidomics Reveals the Homeostasis and Profile of 2 Sphingomyelin from Yak Butter in Normal-Fat Diet-Fed Rats 3 Xin Luo1, Wancheng Sun1, and Yihao Luo1* 4 1College of Agriculture and Animal Husbandry, Qinghai University 5 Address for correspondence: 6 Prof. Yihao Luo 7 College of Agriculture and Animal Husbandry, Qinghai University Ning Da 251, 8 Qinghai Province, Xining 810016, China 9 E-mail:
[email protected] 10 Tel.: +86 13519780623 1 11 Abstract 12 Background:Dietary sphingomyelin was showed to inhibit the uptake of lipids in mice fed with a high-fat diet, however, 13 the effect of sphingomyelin on normal diet was on reported. The current study aims to examine the effects of 14 sphingomyelin extracts from yak butter on hepatic steatosis and inflammation in C57/B6J mice fed with a normal diet. 15 Methods: A UHPLC-QTOF-MS based lipidomics method was utilized to screen the liver metabolites and predict the 16 dominant potential metabolic pathways after sphingomyelin feeding. 17 Results:The results showed that sphingomyelin extracts reduced the accumulation of lipid droplets, suppressed the 18 expression of pro-inflammatory factors IFN -γ, IL-6 and TNF - α, synchronously, promoted the expression of anti- 19 inflammatory factors IL-10, IL-4 and IL-1Ra.