Firenze University Press Caryologia www.fupress.com/caryologia International Journal of Cytology, Cytosystematics and Cytogenetics The technique of Plant DNA Barcoding: potential application in floriculture Citation: A. Giovino, F. Martinelli, A. Perrone (2020) The technique of Plant DNA Barcoding: potential application in floriculture. Caryologia 73(2): 27-37. Antonio Giovino1,*, Federico Martinelli2,*, Anna Perrone3 doi: 10.13128/caryologia-730 1 Council for Agricultural Research and Economics (CREA), Research Centre for Plant Received: December 12, 2019 Protection and Certification (CREA-DC), Bagheria, Italy 2 Department of Biology, University of Florence, Sesto Fiorentino, Florence, 50019, Italy Accepted: April 1, 2020 3 Department of Biological, Chemical and Pharmaceutical Sciences and Technologies Uni- Published: July 31, 2020 versity of Palermo, Viale delle Scienze, Palermo, 90128, Italy *Correspondonding authors. E-mail:
[email protected]; federico.martinelli@ Copyright: © 2020 A. Giovino, F. unifi.it Martinelli, A. Perrone. This is an open access, peer-reviewed article pub- lished by Firenze University Press Abstract. The objective of this work was to assess the ability of the DNA barcoding (http://www.fupress.com/caryologia) approach to identify different taxonomic groups from two flowering plant collec- and distributed under the terms of the tions: 1) the most relevant commercial taxa (nursery production) and 2) Mediterra- Creative Commons Attribution License, nean plants with ornamental attitude (new emerging species). “Core markers”, rbcL which permits unrestricted use, distri- and matK, were adoptedthe identification step of 100 taxa belonging to 20 families. A bution, and reproduction in any medi- third marker, the intergenic spacer trnH-psbA, was also tested, on 74 taxa, when the um, provided the original author and source are credited.