Castanea Sativa Mill.) Normal Conditions (Karacxalı, 2004)
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HORTSCIENCE 50(4):577–581. 2015. Notwithstanding, in Turkey, chestnut growers are not used any treatments to harvested chestnuts to reduce contaminants and water Effects of Different Postharvest Storage losses before storage. Chestnut fruits should be considered as Methods on the Quality Parameters of fresh fruit for storage purposes because they have a 40% to 45% humidity ratio under Chestnuts (Castanea sativa Mill.) normal conditions (Karacxalı, 2004). The hu- midity ratio of fruits should be maintained Engin Ertan1, Esra Erdal, Guls€ um€ Alkan, and Burak E. Algul€ within a certain level to ensure good storage. Department of Horticulture, Agriculture Faculty, Adnan Menderes Cold storage (CS) is the best method to University, Aydın, Turkey ensure appropriate storage conditions for fruit (Soylu, 2004). Chestnuts can withstand Additional index words. chestnut, harvest, storage, quality, biochemical CS because they are not susceptible to dam- Abstract age caused by low temperatures. The best . This trial was initiated in the harvest season of 2010 to determine the effects of preservation conditions for chestnuts are –1 traditional and cold storage on the fruit quality properties of chestnuts during the harvest to –2 °C (Jermini et al., 2006; Rouves and and postharvest periods. Physical and biochemical analyses were conducted on fruit Prunet, 2002) with a relative humidity (RH) samples collected about once every 2 weeks from the middle of September until the end of of 90% (Mencarelli, 2004). December. Specifically, the shell and kernel colors (hue, chroma), water activity (aw), and Harvest and postharvest losses are high total sugar (%), total starch (%), total carbohydrate (%), and tannin (ppm) contents were due to incorrect storage methods (SM) and determined. Under traditional and cold storage conditions, the total sugar content of the nut quality is threatened by pest and diseases. chestnuts increased whereas the total starch content decreased during the storage period. Many SM exist to prolong and maintain the In addition, the maximum tannin content was measured in fruit that was cold stored for quality of nuts but many growers lack the a period of 60 days. technique (Bounous, 2009). Miller (2009) stated that, quality of chest- nut has two major components: characteris- Chestnut (Castanea sativa Mill.) is one of The main storage problems affecting chest- tics and condition. Condition is mainly the most important tree nuts in the world. nuts are the presence of insect worms (Cydia determined by environmental factors, espe- According to the Food and Agriculture Or- splendana Hb, Cydia fagliglandana Zel., and cially postharvest handling, and the time ganization (FAO) Statistical Database, the Curculio elephas Gyll) and the development interval after harvest. Condition is comprised worldwide chestnut production is 1,998,880 of fungi, mainly Cyboria, which blackens the of attributes like moisture content, insect tons. Chestnut fruits are highly regarded and flesh, but also Rhizopus sp., Fusarium sp., infestation, fungal decay (mold), and sugar widely consumed throughout Europe, Amer- Collectotrichum sp., Phomopsis sp. (Breisch, content. The most important environmental ica, and Asia. In addition, chestnuts are one 1993; Washington et al., 1997; Wells and factors are temperature and humidity. These of the most popular nuts in the oriental world. Payne, 1980; Xiao-qing et al., 2009), Aspergil- factors directly affect the moisture and sugar Chestnuts are mainly cultivated in China lus sp., Penicillium sp. (Marinelli et al., 2009), content of chestnut kernels. (1,650,000 tons), Republic of Korea (70,000 Alternaria sp., Trichothecium sp., Botrytis sp., In Turkey, chestnut growers typically store tons), Turkey (59,789 tons), and Italy (52,000 Fusicoccum sp., Phoma sp. (Xiao-qing et al., their crops using traditional methods. For tons) (FAO, 2014). 2009), Sclerotinia sp., and Gibberella sp. example, chestnuts are buried with their burs Chestnuts are rich in starch and sugars, (Donis-Gonzalez et al., 2009a). in a pit under trees in the orchard and are primarily monosaccharides and disaccha- Chestnut quality is measured by external covered with plants such as fern. This is the rides such as sucrose, glucose, fructose, and factors such as color, shape, size, surface most commonly used method, especially in the raffinose (Bernardez et al., 2004; De la blemishes, and molds, which are very im- Aegean Region in the western part of Turkey. Montana Miguelez et al., 2004). In addition, portant for consumer acceptance. Internal Using this method for postharvest storage, the chestnuts differ from other nuts for their low disorders may result from anatomical or moisture level of the chestnut fruit is main- fat content which makes them ideally suited physiological changes such as moisture loss, tained and the sugar content increases from the for high complex carbohydrate and low fat chemical conversion, discoloration, senes- time that the fruit separates from the burs until diets (Bounous, 2009) and they have a unique cence, microorganism attack, cell break- the middle of winter (Soylu, 2004), after which flavor and taste. Due to large proportion of down (physiological decay), or insect injury the nuts are sold. moisture and sugar content, enzyme activity (Upchurch et al., 1993; Wang et al., 2000). Extensive research has been conducted on and pericarp characteristics, the shelf life of Weight losses due to dehydration and in- the nutrient content of chestnuts, effect of chestnuts is very limited (Correia et al., festation by insects and microorganisms are storage on fruit quality properties, and pro- 2009). Therefore, chestnuts are frozen, cold the two main problems in chestnut preserva- tection against microbes. However, there are stored, or dried to extend their storage period. tion (Marinelli et al., 2009; Pinto et al., 2007; no studies on the effects of different SM on However, the nuts have a high moisture Talasila et al., 1995; Tian et al., 2009). the quality parameters of chestnuts. Some content and are therefore susceptible to insect Different postharvest preservation treatments researchers evaluated soluble sugars, starch, damage and fungal decay after harvest, have been used to preserve the nutritional and and polyphenols as quality parameters, of the resulting in high perishability (Miller, 2009; sensory properties of the fruit (Bounous, chestnut kernel in their articles (Cristofori Tzortzakis and Metzidakis, 2012). 2002; Conedera et al., 2005) and to keep the et al., 2009; Tian et al., 2009; Portela et al., fresh commodities against physiological 2009; Vasconcelos et al., 2009). and biological losses during postharvest pe- To maintain the quality and extend the riods, such as water curing (Bassi et al., shelf life of chestnuts, it is essential that they Received for publication 20 Nov. 2014. Accepted 2005; Jermini et al., 2006; Marinelli et al., are adequately stored. This study aimed to for publication 20 Feb. 2015. 2009), low temperature, MA storage (Miller, evaluate the effects of different SM (i.e., This research was supported by The Commission of 2009), and CA storage (Tzortzakis and traditional storage (TS) and CS) and length Scientific Research Project of Adnan Menderes University. The authors would like to thank The Metzidakis, 2012), coatings on the surface of storage (0, 15, 30, 45, and 60 d) on the Commission of Scientific Research Project of Adnan of chestnut fruits (i.e., chitosan, carrageenan) quality attributes of chestnut fruits (i.e., Menderes University, for its meritorious support. (Tian et al., 2009), and various chemical kernel and shell color, water activity, total 1To whom reprint requests should be addressed; sanitizers (i.e., hydrogen peroxide, peracetic sugar, total starch, total carbohydrate, and e-mail [email protected]. acid, ozone) (Donis-Gonzalez et al., 2009b). tannin content). HORTSCIENCE VOL. 50(4) APRIL 2015 577 Materials and Methods The water activity (Aw) in a kernel was Table 1. Physical and biochemical traits of nuts used determined using a water activity device for determining of different storage methods on Chestnut samples and storage treatments. (TH-500, Novosina, Switzerland) at 25 °C. chestnut quality. Chestnut (C. sativa Mill.) samples from the To determine the biochemical character- Trait symbol Trait description ‘‘N-23-1 genotype,’’ which were previously istics, the outer shells of the chestnut fruits Physical characteristics of nuts selected for their high nut quality and high were removed, the fruits were dried in an Shell color yield among natural populations (Ertan oven at 65 °C to a constant weight, and were Sc (L, a, b, hue, chroma) et al., 2007), were collected from orchards then ground. All biochemical analyses were Kernel color located in Kusxcular village (38°02#32.87$ N, replicated three times. Kc (L, a, b, hue, chroma) Aw Activity of water (%) 28°28#34.18$ E, 1060 m altitude) in the The anthrone method was used to de- Nazilli district of Aydın province in western Biochemical characteristics of nuts termine the total sugar content (TSC) and TSC Total sugar content (g/100 g) Turkey, during the second fortnight of Oct. total starch content (TStC) that constituted TStC Total starch content (g/100 g) 2010. The orchards have loamy soil, which is the total carbohydrate content (TCC) Total carbohydrates saltless and slightly alkaline, with good (Morris, 1948) using the Shimadzu ultravio- TCC content (g/100 g) mineral and poor organic matter content let 160-A model spectrophotometer. The TC Tannin content (ppm) (Seferoglu and Ertan, 2009). absorbance values were measured spectro- Well-formed chestnuts without any phys- photometrically at 620 nm and the results are ical injury on the outer skin were selected reported on a dry matter basis. Table 2. F-values from the analysis of variance of the immediately after harvesting. Before the treat- The tannin content (TC) was determined physical and biochemical traits as affected by ments, chestnuts were completely mixed and using Folin–Denis reagent according to storage methods (SM) and days of storage (DS). placed in pure water where the majority of the AOAC (1990) (Canbolat et al., 2007).