Table S3. Differential Expression in Gill Tissue from Arctic Charr (Salvelinus Alpinus) Family 12 After Transfer from Freshwater to Seawater

Table S3. Differential expression in gill tissue from Arctic charr (Salvelinus alpinus) family 12 after transfer from freshwater to seawater.

AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 1 510543 < 1.0E-15 8.7 gi_12805155, AAH02035.1, Unknown (protein for MGC:5947) [Mus musculus] 100 1.35E-135 394 2 323017 < 1.0E-15 8.0 gi_213513042, NP_001134270.1, fucosyltransferase 9 [Salmo salar] >gi_209731992, ACI66865.1, 92 4.79E-42 161 Alpha-1,3-fucosyltransferase [Salmo salar] 3 499923 < 1.0E-15 7.9 gi_197631823, ACH70635.1, cytochrome c-like [Salmo salar] >gi_209738490, ACI70114.1, Cytochrome 98 8.02E-64 213 c [Salmo salar] >gi_221219870, ACM08596.1, Cytochrome c [Salmo salar] >gi_221220596, ACM08959.1, Cytochrome c [Salmo salar] >gi_225703780, ACO07736.1, Cytochrome c [Oncorhynchus mykiss] >gi_225705644, ACO08668.1, Cytochrome c [Oncorhynchus mykiss] >gi_303660333, ADM15993.1, Cytochrome c [Salmo salar]

4 99748 < 1.0E-15 7.6 gi_348545563, XP_003460249.1, PREDICTED: hypothetical protein LOC100708591 [Oreochromis 67 2.78E-157 479 niloticus] 5 41921 < 1.0E-15 6.7 gi_209737600, ACI69669.1, Caspase-14 precursor [Salmo salar] 75 1.48E-52 189 6 225544 < 1.0E-15 6.4 gi_83312446, YP_422710.1, Calphotin; microtubule-associated protein 4 [Magnetospirillum magneticum 70 7.52E-05 55 AMB-1] >gi_82947287, BAE52151.1, Calphotin; Microtubule-associated protein 4 [Magnetospirillum magneticum AMB-1]

7 151773 < 1.0E-15 6.3 gi_348517158, XP_003446102.1, PREDICTED: kallikrein-2-like [Oreochromis niloticus] 50 3.49E-28 118 8 293079 < 1.0E-15 6.3 gi_301607131, XP_002933173.1, PREDICTED: hypothetical protein LOC100488659 [Xenopus (Silurana) 56 4.17E-25 64 tropicalis] 9 604942 < 1.0E-15 6.2 gi_394933887, BAM29295.1, Zen 1 [Dermatophagoides farinae] 59 1.47E-09 67 10 511409 5.4E-05 6.2 gi_209734584, ACI68161.1, Caspase-14 precursor [Salmo salar] 76 7.37E-75 246 11 193909 1.4E-04 6.1 gi_209732928, ACI67333.1, NADH dehydrogenase 1 alpha subcomplex subunit 9, mitochondrial 98 1.37E-159 473 precursor [Salmo salar] >gi_223647086, ACN10301.1, NADH dehydrogenase 1 alpha subcomplex subunit 9, mitochondrial precursor [Salmo salar] >gi_223672959, ACN12661.1, NADH dehydrogenase 1 alpha subcomplex subunit 9, mitochondrial precursor [Salmo salar]

12 592962 3.8E-08 5.9 gi_6755574, NP_035544.1, antileukoproteinase precursor [Mus musculus] >gi_2829839, P97430.1, 100 4.05E-100 297 SLPI_MOUSE RecName: Full=Antileukoproteinase; Short=ALP; AltName: Full=Secretory leukocyte protease inhibitor; Flags: Precursor >gi_6606529, AAF19194.1, AF205374_1 secretory leukocyte protease inhibitor [Mus musculus] >gi_1763263, AAC53047.1, secretory leukocyte protease inhibitor [Mus musculus] >gi_1945383, AAC53140.1, secretory leukocyte protease inhibitor [Mus musculus] >gi_1945451, AAC53394.1, secretory leukoprotease inhibitor [Mus musculus] >gi_4100899, AAD09307.1, secretory leukoprotease inhibitor [Mus musculus] >gi_20306996, AAH28509.1, Secretory leukocyte peptidase inhibitor [Mus musculus] >gi_148674431, EDL06378.1, secretory leukocyte peptidase inhibitor, isoform CRA_a [Mus musculus] >gi_148674432, EDL06379.1, secretory leukocyte peptidase inhibitor, isoform CRA_a [Mus musculus]

13 41925 6.2E-05 5.9 gi_209737600, ACI69669.1, Caspase-14 precursor [Salmo salar] 76 1.21E-71 237 14 217197 2.3E-13 5.8 gi_83282445, XP_729774.1, hypothetical protein [Plasmodium yoelii yoelii 17XNL] >gi_23488517, 57 7.23E-13 74 EAA21339.1, hypothetical protein [Plasmodium yoelii yoelii] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 15 413286 2.4E-11 5.7 gi_156352243, XP_001622673.1, predicted protein [Nematostella vectensis] >gi_156209261, 76 7.38E-09 63 EDO30573.1, predicted protein [Nematostella vectensis] 16 192325 3.8E-04 5.4 gi_410896384, XP_003961679.1, PREDICTED: splicing factor 3B subunit 1-like [Takifugu rubripes] 96 < 1.0E-180 2183 17 590957 1.9E-02 5.4 gi_402869608, XP_003898844.1, PREDICTED: immunoglobulin J chain isoform 1 [Papio anubis] 83 1.73E-56 142 18 563373 2.0E-05 5.4 gi_348525450, XP_003450235.1, PREDICTED: hypothetical protein LOC100694880 [Oreochromis 68 2.61E-52 198 niloticus] 19 522943 1.9E-05 5.4 gi_348500316, XP_003437719.1, PREDICTED: protein LSM14 homolog A-like [Oreochromis niloticus] 77 2.03E-131 424 20 201288 < 1.0E-15 5.4 gi_327287605, XP_003228519.1, PREDICTED: putative adenosylhomocysteinase 3-like [Anolis 97 5.22E-60 220 carolinensis] 21 684033 7.4E-04 5.3 gi_348531282, XP_003453139.1, PREDICTED: pecanex-like protein C14orf135 homolog [Oreochromis 82 < 1.0E-180 722 niloticus] 22 640643 4.8E-05 5.3 gi_229366190, ACQ58075.1, Kallikrein-8 precursor [Anoplopoma fimbria] 50 1.15E-26 89 23 547655 1.7E-02 5.2 gi_148725645, CAN87719.1, novel protein (zgc:91911) [Danio rerio] 98 2.74E-107 327 24 642187 8.1E-03 5.1 gi_410920215, XP_003973579.1, PREDICTED: cell division cycle-associated protein 7-like [Takifugu 70 2.25E-130 394 rubripes] 25 480417 2.5E-03 5.1 gi_357483895, XP_003612234.1, ATP synthase subunit a chloroplastic [Medicago truncatula] 78 5.52E-07 56 >gi_355513569, AES95192.1, ATP synthase subunit a chloroplastic [Medicago truncatula] 26 480420 5.7E-04 4.9 gi_390362987, XP_788663.3, PREDICTED: histamine H3 receptor-like [Strongylocentrotus purpuratus] 65 4.34E-06 54 27 50943 1.2E-02 4.9 gi_259089145, NP_001158610.1, deoxycytidine kinase [Oncorhynchus mykiss] >gi_225705244, 100 2.74E-88 278 ACO08468.1, Deoxycytidine kinase [Oncorhynchus mykiss] 28 60409 1.6E-03 4.9 gi_16549028, CAC82808.1, inducible nitric oxide synthase [Oncorhynchus mykiss] 98 < 1.0E-180 1453 29 89387 1.3E-13 4.9 gi_157278121, NP_001098159.1, transcription factor C-MYB [Oryzias latipes] >gi_40714443, 73 < 1.0E-180 669 BAD06940.1, transcription factor C-MYB [Oryzias latipes] 30 286882 2.9E-03 4.7 gi_260794874, XP_002592432.1, hypothetical protein BRAFLDRAFT_67299 [Branchiostoma floridae] 66 4.18E-04 53 >gi_229277651, EEN48443.1, hypothetical protein BRAFLDRAFT_67299 [Branchiostoma floridae] 31 561397 1.8E-02 4.7 gi_113678175, NP_001038335.1, uncharacterized protein LOC558619 precursor [Danio rerio] 45 5.50E-15 82 >gi_94732333, CAK11037.1, novel protein [Danio rerio] 32 354384 1.5E-06 4.6 gi_303227986, NP_001037801.3, chromosome transmission fidelity protein 8 homolog [Danio rerio] 89 8.39E-43 132 >gi_94733486, CAK04527.1, novel protein [Danio rerio] >gi_213624828, AAI71641.1, Si:ch211-218c6.5 protein [Danio rerio]

33 496824 6.0E-04 4.4 gi_85679686, ABC72122.1, myeloperoxidase [Siniperca chuatsi] 84 < 1.0E-180 1111 34 645707 2.3E-03 4.4 gi_189530913, XP_001339622.2, PREDICTED: hypothetical protein LOC799244 [Danio rerio] 83 < 1.0E-180 653 35 60206 8.2E-05 4.3 gi_225706360, ACO09026.1, Ubiquitin-conjugating enzyme E2 G1 [Osmerus mordax] 98 7.71E-104 332 36 677559 1.0E-02 4.2 gi_410896740, XP_003961857.1, PREDICTED: discoidin, CUB and LCCL domain-containing protein 2- 79 < 1.0E-180 870 like [Takifugu rubripes] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 37 496803 9.2E-09 4.1 gi_224613258, ACN60208.1, Myeloperoxidase precursor [Salmo salar] 86 1.46E-168 511 38 707813 1.6E-03 4.1 gi_222527286, YP_002571757.1, laminin G, sub domain 2 [Chloroflexus sp. Y-400-fl] >gi_222451165, 40 1.52E-29 136 ACM55431.1, laminin G, sub domain 2 [Chloroflexus sp. Y-400-fl] 39 588379 2.5E-02 4.1 gi_212656619, ACJ36222.1, forkhead box P1b [Ctenopharyngodon idella] 89 < 1.0E-180 716 40 201196 < 1.0E-15 4.1 gi_209736498, ACI69118.1, Transmembrane and TPR repeat-containing protein 4 [Salmo salar] 77 1.21E-18 93 41 201257 < 1.0E-15 3.9 gi_355560983, EHH17669.1, hypothetical protein EGK_14124, partial [Macaca mulatta] 91 < 1.0E-180 586 42 524561 1.9E-02 3.9 gi_348506772, XP_003440931.1, PREDICTED: 2-oxoglutarate receptor 1-like [Oreochromis niloticus] 79 1.03E-119 365 43 586375 4.5E-03 3.8 gi_94732587, CAK05072.1, novel protein similar to vertebrate potassium inwardly-rectifying channel, 98 9.26E-83 263 subfamily J, member 3 (KCNJ3) [Danio rerio] 44 497949 3.2E-05 3.8 gi_348503347, XP_003439226.1, PREDICTED: enhancer of polycomb homolog 1-like [Oreochromis 71 3.34E-78 175 niloticus] 45 666299 5.2E-02 3.7 gi_225716736, ACO14214.1, HRAS-like suppressor 2 [Esox lucius] 55 1.90E-22 102 46 277922 3.8E-02 3.6 gi_225716830, ACO14261.1, CD209 antigen-like protein A [Esox lucius] 68 1.33E-34 136 47 90802 1.6E-06 3.4 gi_410903199, XP_003965081.1, PREDICTED: keratin, type I cytoskeletal 50 kDa-like [Takifugu 84 2.73E-81 195 rubripes] 48 151768 3.3E-02 3.4 gi_308210828, NP_001184098.1, kallikrein-4 precursor [Canis lupus familiaris] >gi_307827342, 47 2.41E-16 84 ADN94676.1, kallikrein-related peptidase 4 precursor [Canis lupus familiaris] 49 754898 9.5E-03 3.4 gi_255982777, CAP08938.1, fish virus induced TRIM protein [Oncorhynchus mykiss] 80 1.67E-116 364 50 617198 9.5E-05 3.3 gi_348517672, XP_003446357.1, PREDICTED: pumilio homolog 2 isoform 1 [Oreochromis niloticus] 91 < 1.0E-180 923 51 584736 3.3E-03 3.3 gi_213514156, NP_001134448.1, E3 ubiquitin-protein ligase RNF138 [Salmo salar] >gi_209733384, 83 6.07E-46 165 ACI67561.1, E3 ubiquitin-protein ligase RNF138 [Salmo salar] 52 316923 2.3E-10 3.2 gi_326677981, XP_002666131.2, PREDICTED: hypothetical protein LOC100331949 [Danio rerio] 57 5.12E-06 57 53 2452 1.6E-13 3.2 gi_292658798, NP_001167620.1, cytochrome P450, family 1, subfamily B, polypeptide 1 [Oncorhynchus 98 < 1.0E-180 1072 mykiss] >gi_290576814, ADD51159.1, cytochrome P450 family 1 subfamily B polypeptide 1 [Oncorhynchus mykiss]

54 338934 1.1E-12 3.1 gi_76153175, AAX24831.2, SJCHGC03009 protein [Schistosoma japonicum] 65 2.20E-13 79 55 369899 < 1.0E-15 3.1 gi_348535832, XP_003455402.1, PREDICTED: large subunit GTPase 1 homolog [Oreochromis niloticus] 82 < 1.0E-180 901 56 316080 < 1.0E-15 3.0 gi_76153402, AAX25032.2, SJCHGC04714 protein [Schistosoma japonicum] 61 2.74E-05 50 57 275343 < 1.0E-15 3.0 gi_62122915, NP_001014379.1, uncharacterized protein LOC541543 [Danio rerio] >gi_61403165, 54 3.48E-32 88 AAH91811.1, Zgc:112970 [Danio rerio] 58 162163 1.9E-06 3.0 gi_348535132, XP_003455055.1, PREDICTED: hypothetical protein LOC100705794 [Oreochromis 65 8.84E-43 171 niloticus] 59 535967 1.7E-04 3.0 gi_209736726, ACI69232.1, Probable E3 ubiquitin-protein ligase RNF144A-A [Salmo salar] 75 3.27E-42 160 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 60 244895 9.2E-11 3.0 gi_192455690, NP_001122275.1, uncharacterized protein LOC798684 [Danio rerio] >gi_190338851, 61 3.56E-17 89 AAI62677.1, Hypothetical LOC798684 [Danio rerio] >gi_190338853, AAI62685.1, Hypothetical LOC798684 [Danio rerio]

61 316878 5.7E-03 2.9 gi_60687998, AAX30332.1, SJCHGC03023 protein [Schistosoma japonicum] 85 2.57E-14 72 62 551487 4.4E-07 2.9 gi_209737466, ACI69602.1, C-C motif chemokine 19 precursor [Salmo salar] >gi_303662007, 79 1.46E-39 148 ADM16062.1, C-C motif chemokine 19 precursor [Salmo salar] 63 151077 9.6E-13 2.9 gi_209733396, ACI67567.1, Gamma-interferon-inducible lysosomal thiol reductase precursor [Salmo 96 9.06E-177 505 salar] >gi_223647174, ACN10345.1, Gamma-interferon-inducible lysosomal thiol reductase precursor [Salmo salar] >gi_223673047, ACN12705.1, Gamma-interferon-inducible lysosomal thiol reductase precursor [Salmo salar] 64 262843 3.0E-02 2.8 gi_402864226, XP_003896375.1, PREDICTED: sterile alpha motif domain-containing protein 9 isoform 1 52 4.80E-55 94 [Papio anubis] >gi_402864228, XP_003896376.1, PREDICTED: sterile alpha motif domain-containing protein 9 isoform 2 [Papio anubis]

65 134805 < 1.0E-15 2.8 gi_348529836, XP_003452418.1, PREDICTED: CUB and zona pellucida-like domain-containing protein 76 8.20E-143 451 1-like [Oreochromis niloticus] 66 601072 < 1.0E-15 2.8 gi_33331453, AAQ10900.1, cytochrome P450 1A [Salvelinus namaycush] 100 < 1.0E-180 658 67 301121 3.7E-11 2.8 gi_225706062, ACO08877.1, Tumor necrosis factor receptor superfamily member 14 precursor [Osmerus 63 1.81E-58 203 mordax] 68 275338 4.5E-09 2.7 gi_62122915, NP_001014379.1, uncharacterized protein LOC541543 [Danio rerio] >gi_61403165, 57 1.87E-34 94 AAH91811.1, Zgc:112970 [Danio rerio] 69 736165 9.2E-07 2.7 gi_326675606, XP_003200393.1, PREDICTED: hypothetical protein LOC449979 [Danio rerio] 69 < 1.0E-180 817 70 353417 1.4E-04 2.7 gi_317419374, CBN81411.1, Alpha-tectorin [Dicentrarchus labrax] 81 < 1.0E-180 2897 71 402582 2.0E-06 2.7 gi_221220216, ACM08769.1, Apoptosis-associated speck-like protein containing a CARD [Salmo salar] 82 1.78E-32 133 >gi_223646320, ACN09918.1, Apoptosis-associated speck-like protein containing a CARD [Salmo salar] >gi_223672167, ACN12265.1, Apoptosis-associated speck-like protein containing a CARD [Salmo salar]

72 638648 1.5E-02 2.7 gi_156368782, XP_001627871.1, predicted protein [Nematostella vectensis] >gi_156214832, 66 1.89E-16 82 EDO35808.1, predicted protein [Nematostella vectensis] 73 52343 9.2E-06 2.6 gi_410931580, XP_003979173.1, PREDICTED: uncharacterized protein LOC101062517 [Takifugu 84 < 1.0E-180 1007 rubripes] 74 31554 8.6E-08 2.6 gi_348504098, XP_003439599.1, PREDICTED: olfactomedin-4-like [Oreochromis niloticus] 80 2.90E-175 514 75 601086 1.1E-13 2.6 gi_33331451, AAQ10899.1, cytochrome P450 1A [Salvelinus fontinalis] 100 < 1.0E-180 1012 76 134787 1.4E-06 2.6 gi_326675923, XP_001340509.4, PREDICTED: oncoprotein-induced transcript 3 protein-like [Danio rerio] 76 4.22E-45 169 77 64947 < 1.0E-15 2.6 gi_302039713, BAJ13362.1, sodium/potassium-transporting ATPase subunit alpha-1b [Oncorhynchus 98 < 1.0E-180 1886 masou] 78 524585 5.7E-05 2.5 gi_348506772, XP_003440931.1, PREDICTED: 2-oxoglutarate receptor 1-like [Oreochromis niloticus] 80 5.43E-101 326 79 689852 1.1E-02 2.5 gi_33413534, AAN02165.1, reproduction regulator 2 [Epinephelus coioides] 67 2.90E-17 86 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 80 317022 9.1E-05 2.5 gi_326677981, XP_002666131.2, PREDICTED: hypothetical protein LOC100331949 [Danio rerio] 64 1.52E-05 56 81 286857 5.0E-02 2.5 gi_326677981, XP_002666131.2, PREDICTED: hypothetical protein LOC100331949 [Danio rerio] 57 1.16E-08 63 82 317020 1.3E-07 2.5 gi_326668958, XP_002662647.2, PREDICTED: hypothetical protein LOC100333859 [Danio rerio] 55 5.13E-07 64 83 656347 3.3E-02 2.4 gi_348541829, XP_003458389.1, PREDICTED: acid sphingomyelinase-like phosphodiesterase 3a-like 76 3.76E-40 157 [Oreochromis niloticus] 84 21871 6.1E-03 2.4 gi_348538726, XP_003456841.1, PREDICTED: coiled-coil domain-containing protein 61-like isoform 2 81 8.08E-136 421 [Oreochromis niloticus] 85 134822 1.4E-03 2.4 gi_326675919, XP_002665352.2, PREDICTED: hypothetical protein LOC100332877 [Danio rerio] 75 8.98E-103 321 86 134827 2.7E-03 2.4 gi_326675919, XP_002665352.2, PREDICTED: hypothetical protein LOC100332877 [Danio rerio] 81 1.53E-36 150 87 664636 4.4E-11 2.4 gi_220679572, CAX12823.1, novel protein similar to vertebrate tripartite motif-containing family [Danio 73 2.28E-89 296 rerio] 88 229116 9.6E-05 2.4 gi_156615292, XP_001647513.1, predicted protein [Nematostella vectensis] >gi_156214746, 60 2.99E-08 63 EDO35724.1, predicted protein [Nematostella vectensis] 89 343460 7.6E-05 2.4 gi_156408850, XP_001642069.1, predicted protein [Nematostella vectensis] >gi_156229210, 41 1.74E-17 91 EDO50006.1, predicted protein [Nematostella vectensis] 90 282331 3.2E-12 2.3 gi_76153153, AAX24812.2, SJCHGC04011 protein [Schistosoma japonicum] 71 9.59E-13 75 91 367176 6.0E-03 2.3 gi_47225343, CAG09843.1, unnamed protein product [Tetraodon nigroviridis] >gi_151427554, tpd, 90 < 1.0E-180 942 FAA00334.1, TPA: predicted NADPH oxidase-1 [Tetraodon nigroviridis] 92 482759 1.2E-02 2.3 gi_403242711, AFR33114.1, megalocytivirus-induced protein 1 [Cynoglossus semilaevis] 51 1.02E-85 291 93 402558 2.2E-03 2.3 gi_348543961, XP_003459450.1, PREDICTED: sterile alpha motif domain-containing protein 9-like 69 1.04E-32 126 [Oreochromis niloticus] 94 69250 1.3E-02 2.3 gi_348509696, XP_003442383.1, PREDICTED: putative sodium-coupled neutral amino acid transporter 89 < 1.0E-180 612 7-like [Oreochromis niloticus] 95 320706 < 1.0E-15 2.3 gi_34529777, BAC85768.1, unnamed protein product [Homo sapiens] >gi_34530108, BAC85836.1, 51 4.48E-20 102 unnamed protein product [Homo sapiens] 96 251482 6.1E-03 2.3 gi_344245311, EGW01415.1, Tripartite motif-containing protein 39 [Cricetulus griseus] 65 1.35E-41 163 97 559064 1.5E-02 2.3 gi_326663954, XP_686125.4, PREDICTED: hypothetical protein LOC557879 [Danio rerio] 67 1.54E-172 532 98 15915 1.5E-02 2.3 gi_213513542, NP_001135127.1, omega-amidase NIT2 [Salmo salar] >gi_209731714, ACI66726.1, 69 5.49E-38 142 Nitrilase homolog 2 [Salmo salar] 99 237961 3.9E-02 2.3 gi_209733848, ACI67793.1, Oocyte zinc finger protein XlCOF20 [Salmo salar] 87 2.48E-57 198 100 278159 4.2E-08 2.3 gi_198285635, ACH85356.1, hypothetical protein [Salmo salar] 81 1.53E-16 88 101 377 6.7E-07 2.2 gi_300795671, NP_998587.2, UDP glucuronosyltransferase 1 family polypeptide a3 precursor [Danio 89 < 1.0E-180 414 rerio] 102 38735 1.0E-03 2.2 gi_209962463, ACJ02099.1, FGF2 [Salmo salar] 85 5.97E-05 44 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 103 416048 5.2E-02 2.2 gi_150378445, NP_001092900.1, protein asteroid homolog 1 [Danio rerio] >gi_123889254, Q1LYL8.1, 55 1.98E-47 182 ASTE1_DANRE RecName: Full=Protein asteroid homolog 1 >gi_94732441, CAK05130.1, novel protein similar to human HT001 protein (HT001) [Danio rerio] >gi_190339910, AAI63487.1, Asteroid homolog 1 (Drosophila) [Danio rerio] >gi_190339914, AAI63493.1, Asteroid homolog 1 (Drosophila) [Danio rerio]

104 283145 2.4E-02 2.2 gi_148362154, ABQ59677.1, Pit54 [Salmo salar] >gi_260182166, ACX35595.1, PIT54 [Salmo salar] 64 1.09E-87 280 105 242888 3.4E-02 2.1 gi_213512428, NP_001133755.1, Transmembrane protein 60 [Salmo salar] >gi_209155224, 98 4.09E-81 272 ACI33844.1, Transmembrane protein 60 [Salmo salar] >gi_209735886, ACI68812.1, Transmembrane protein 60 [Salmo salar]

106 325758 6.9E-03 2.1 gi_213511522, NP_001134518.1, CD209 antigen-like protein D [Salmo salar] >gi_209733950, 59 5.81E-64 217 ACI67844.1, CD209 antigen-like protein D [Salmo salar] 107 535277 1.0E-06 2.1 gi_209736786, ACI69262.1, Cytochrome c oxidase subunit 4 isoform 2, mitochondrial precursor [Salmo 98 4.54E-120 362 salar] 108 320889 2.7E-02 2.0 gi_67772182, AAY79343.1, unknown protein [Siniperca chuatsi] 63 1.99E-26 112 109 22718 5.6E-03 2.0 gi_410930476, XP_003978624.1, PREDICTED: calpain-3-like [Takifugu rubripes] 67 2.04E-68 238 110 244901 2.6E-08 2.0 gi_348535790, XP_003455381.1, PREDICTED: ephrin type-A receptor 2 [Oreochromis niloticus] 89 < 1.0E-180 750 111 703154 2.6E-02 2.0 gi_348516612, XP_003445832.1, PREDICTED: hypothetical protein LOC100693248 [Oreochromis 77 1.57E-164 507 niloticus] 112 536509 1.7E-03 2.0 gi_221220730, ACM09026.1, GTPase IMAP family member 7 [Salmo salar] 81 3.78E-109 338 113 258054 < 1.0E-15 2.0 gi_209974067, ACJ03990.1, ataxia telangiectasia mutated [Danio rerio] 76 1.27E-41 164 114 664014 4.7E-02 1.9 gi_255982793, CAP08946.1, fish virus induced TRIM protein [Oncorhynchus mykiss] 86 5.45E-62 143 115 613564 3.7E-06 1.9 gi_213514156, NP_001134448.1, E3 ubiquitin-protein ligase RNF138 [Salmo salar] >gi_209733384, 82 2.80E-130 383 ACI67561.1, E3 ubiquitin-protein ligase RNF138 [Salmo salar] 116 242274 5.1E-02 1.9 gi_125819496, XP_690360.2, PREDICTED: hypothetical protein LOC561863 [Danio rerio] 60 1.82E-33 151 117 664404 3.6E-02 1.9 gi_125811426, XP_001335256.1, PREDICTED: hypothetical protein LOC795145 [Danio rerio] 60 1.87E-68 233 118 217178 5.0E-02 1.8 gi_63101284, AAH95638.1, Asph protein, partial [Danio rerio] 85 4.98E-30 130 119 402960 1.2E-02 1.8 gi_60688018, AAX30342.1, SJCHGC03036 protein [Schistosoma japonicum] 64 5.23E-11 60 120 324169 1.0E-10 1.8 gi_5579035, CAB51372.1, transposase [Pleuronectes platessa] 58 2.99E-04 48 121 162523 2.9E-02 1.8 gi_41351177, AAH65621.1, Zgc:77182 [Danio rerio] 93 9.49E-155 461 122 176046 4.4E-03 1.8 gi_348510333, XP_003442700.1, PREDICTED: LOW QUALITY PROTEIN: death-inducer obliterator 1- 39 5.79E-15 89 like [Oreochromis niloticus] 123 316957 1.5E-10 1.8 gi_301604810, XP_002932065.1, PREDICTED: hypothetical protein LOC100488389 [Xenopus (Silurana) 63 3.83E-14 80 tropicalis] 124 537214 5.8E-03 1.8 gi_209734488, ACI68113.1, Cyclin-dependent kinases regulatory subunit 1 [Salmo salar] 100 6.10E-36 137 >gi_209735050, ACI68394.1, Cyclin-dependent kinases regulatory subunit 1 [Salmo salar] 125 44912 4.3E-04 1.7 gi_410919459, XP_003973202.1, PREDICTED: protein lifeguard 2-like [Takifugu rubripes] 89 2.25E-123 378 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 126 161135 1.4E-09 1.7 gi_410913401, XP_003970177.1, PREDICTED: SH2 domain-containing protein 4A-like [Takifugu 60 1.95E-36 145 rubripes] 127 264401 < 1.0E-15 1.7 gi_404250106, AFR53956.1, transposase [Cloning vector pT2/Gene-Trap] 88 1.46E-164 367 128 86162 4.6E-02 1.7 gi_348544532, XP_003459735.1, PREDICTED: tumor necrosis factor receptor superfamily member 21- 57 6.65E-142 450 like [Oreochromis niloticus] 129 115768 3.4E-02 1.7 gi_292626081, XP_002666225.1, PREDICTED: piggyBac transposable element-derived protein 4-like 70 6.11E-171 509 [Danio rerio] 130 716682 5.0E-09 1.7 gi_221220752, ACM09037.1, C-C motif chemokine 20 precursor [Salmo salar] >gi_221221158, 95 3.61E-34 130 ACM09240.1, C-C motif chemokine 20 precursor [Salmo salar] 131 601351 4.7E-02 1.7 gi_213514880, NP_001134597.1, C-C motif chemokine 8 precursor [Salmo salar] >gi_209734562, 96 4.74E-24 103 ACI68150.1, C-C motif chemokine 8 precursor [Salmo salar] 132 87969 1.2E-02 1.7 gi_213514736, NP_001133256.1, Coiled-coil domain-containing protein 82 [Salmo salar] >gi_209147924, 99 2.36E-131 403 ACI32912.1, Coiled-coil domain-containing protein 82 [Salmo salar] 133 415094 4.1E-05 1.7 gi_185132194, NP_001118214.1, cytochrome P450 2K5 [Oncorhynchus mykiss] >gi_8101501, 96 < 1.0E-180 872 AAF72563.1, AF151524_1 cytochrome P450 2K5 [Oncorhynchus mykiss] 134 263649 3.6E-04 1.6 gi_76153175, AAX24831.2, SJCHGC03009 protein [Schistosoma japonicum] 66 1.09E-23 102 135 126240 6.2E-03 1.6 gi_51468063, NP_001003887.1, treslin [Danio rerio] >gi_82085445, Q6DRL4.1, TICRR_DANRE 53 2.58E-132 448 RecName: Full=Treslin; AltName: Full=TopBP1-interacting checkpoint and replication regulator; AltName: Full=TopBP1-interacting, replication-stimulating protein >gi_49618957, AAT68063.1, RIKEN cDNA 5730590G19-like [Danio rerio]

136 120178 2.4E-03 1.6 gi_47216853, CAG11660.1, unnamed protein product [Tetraodon nigroviridis] 84 2.67E-124 397 137 96136 1.9E-02 1.6 gi_404250106, AFR53956.1, transposase [Cloning vector pT2/Gene-Trap] 91 2.24E-32 134 138 525464 2.5E-08 1.6 gi_395740045, XP_003777356.1, PREDICTED: uncharacterized protein LOC100938603 [Pongo abelii] 62 2.04E-07 61 139 212623 7.1E-08 1.6 gi_357575246, AET85182.1, TC1-like transposase [Cyprinus carpio] 78 4.47E-65 148 140 162185 1.1E-03 1.6 gi_348535132, XP_003455055.1, PREDICTED: hypothetical protein LOC100705794 [Oreochromis 59 8.33E-79 267 niloticus] 141 361169 < 1.0E-15 1.6 gi_345786882, XP_533791.3, PREDICTED: LOW QUALITY PROTEIN: interleukin-17 receptor B [Canis 49 1.61E-13 83 lupus familiaris] 142 477715 2.7E-02 1.6 gi_34529777, BAC85768.1, unnamed protein product [Homo sapiens] >gi_34530108, BAC85836.1, 60 8.19E-40 156 unnamed protein product [Homo sapiens] 143 403700 3.2E-02 1.6 gi_225716102, ACO13897.1, Peroxisomal trans-2-enoyl-CoA reductase [Esox lucius] 96 3.01E-24 111 144 567435 3.3E-02 1.6 gi_213511702, NP_001134263.1, CD276 antigen precursor [Salmo salar] >gi_209731944, ACI66841.1, 52 2.97E-27 120 CD276 antigen precursor [Salmo salar] 145 632917 3.0E-02 1.6 gi_209736992, ACI69365.1, Mitochondrial ribosomal protein 63 [Salmo salar] 100 6.72E-36 137 146 699049 1.3E-02 1.5 gi_348541645, XP_003458297.1, PREDICTED: PR domain zinc finger protein 1-like [Oreochromis 77 7.69E-116 371 niloticus] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 147 667273 2.0E-03 1.5 gi_348534757, XP_003454868.1, PREDICTED: succinate receptor 1-like [Oreochromis niloticus] 79 1.10E-112 342 148 694198 3.0E-02 1.5 gi_225717120, ACO14406.1, Chymase precursor [Esox lucius] 71 4.28E-71 237 149 403713 1.8E-02 1.5 gi_225716102, ACO13897.1, Peroxisomal trans-2-enoyl-CoA reductase [Esox lucius] 97 3.80E-77 250 150 391068 9.7E-03 1.5 gi_223649266, ACN11391.1, Transposable element Tcb1 transposase [Salmo salar] 80 3.99E-13 75 151 638026 3.0E-04 1.5 gi_213513191, NP_001134157.1, Carbonic anhydrase 12 precursor [Salmo salar] >gi_209731112, 87 2.36E-56 188 ACI66425.1, Carbonic anhydrase 12 precursor [Salmo salar] >gi_303657463, ADM15876.1, Carbonic anhydrase 12 precursor [Salmo salar]

152 570526 5.1E-02 1.5 gi_189516081, XP_001341582.2, PREDICTED: glutamate [NMDA] receptor-associated protein 1 [Danio 85 9.24E-93 295 rerio] 153 472624 4.0E-02 1.5 gi_185134290, NP_001117918.1, glycogen synthase kinase binding protein [Oncorhynchus mykiss] 81 2.53E-29 124 >gi_37544637, AAM44075.1, guanylate-binding protein [Oncorhynchus mykiss] 154 101054 3.2E-12 1.5 gi_171916109, NP_001116443.1, myxovirus (influenza virus) resistance G [Danio rerio] >gi_48427584, 69 < 1.0E-180 583 CAD67761.2, MxG protein [Danio rerio] 155 758304 3.7E-02 1.5 gi_122891324, CAM13025.1, novel protein [Danio rerio] 63 6.50E-30 127 156 44221 < 1.0E-15 1.4 gi_47213251, CAF92912.1, unnamed protein product [Tetraodon nigroviridis] 57 7.00E-70 259 157 607785 2.5E-06 1.4 gi_348537290, XP_003456128.1, PREDICTED: nucleolar protein 6-like [Oreochromis niloticus] 86 < 1.0E-180 693 158 161758 1.3E-06 1.4 gi_348525206, XP_003450113.1, PREDICTED: cyclin-F-like [Oreochromis niloticus] 84 < 1.0E-180 1033 159 402603 6.1E-03 1.4 gi_348520258, XP_003447645.1, PREDICTED: sterile alpha motif domain-containing protein 9 45 1.84E-61 242 [Oreochromis niloticus] 160 39230 2.1E-02 1.4 gi_348514884, XP_003444970.1, PREDICTED: probable G-protein coupled receptor 61-like 77 4.85E-155 474 [Oreochromis niloticus] 161 140743 2.4E-02 1.4 gi_348509187, XP_003442133.1, PREDICTED: hsp90 co-chaperone Cdc37-like [Oreochromis niloticus] 88 5.00E-166 503 162 501767 2.0E-05 1.4 gi_326670954, XP_001336175.4, PREDICTED: interferon-induced protein 44 [Danio rerio] 70 1.07E-55 143 163 361191 8.7E-06 1.4 gi_301787217, XP_002929026.1, PREDICTED: interleukin-17 receptor B-like [Ailuropoda melanoleuca] 43 7.41E-04 51 164 632057 2.6E-02 1.4 gi_297206854, NP_001171962.1, cytochrome P450 family 1 subfamily C polypeptide 3 [Oncorhynchus 98 < 1.0E-180 1027 mykiss] >gi_290576818, ADD51161.1, cytochrome P450 family 1 subfamily C polypeptide 3 [Oncorhynchus mykiss] 165 497279 1.6E-03 1.4 gi_224613524, ACN60341.1, Calreticulin precursor [Salmo salar] 100 1.79E-63 210 166 701989 1.4E-03 1.4 gi_147905506, NP_001083026.1, putative ferric-chelate reductase 1 precursor [Danio rerio] 62 1.90E-17 96 >gi_166198777, A4QP81.1, FRRS1_DANRE RecName: Full=Putative ferric-chelate reductase 1 >gi_141795735, AAI39693.1, Zgc:163022 protein [Danio rerio]

167 363770 2.6E-05 1.4 gi_125845863, XP_685228.2, PREDICTED: serine/threonine-protein kinase Nek1 [Danio rerio] 47 2.17E-06 59 168 82724 7.2E-04 1.3 gi_67514545, NP_001005931.2, ras-related protein R-Ras [Danio rerio] >gi_66910346, AAH96943.1, 97 6.20E-107 346 Related RAS viral (r-ras) oncogene homolog [Danio rerio] >gi_182891132, AAI63945.1, Rras protein [Danio rerio] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 169 132909 6.1E-03 1.3 gi_62079626, AAX61159.1, selenoprotein W2a [Oreochromis mossambicus] 82 7.01E-34 132 170 574056 3.7E-02 1.3 gi_410916553, XP_003971751.1, PREDICTED: basic leucine zipper transcriptional factor ATF-like 79 1.16E-43 161 [Takifugu rubripes] 171 607729 8.3E-07 1.3 gi_359485606, XP_003633295.1, PREDICTED: uncharacterized protein LOC100853570 [Vitis vinifera] 35 2.18E-10 71 172 264358 3.9E-05 1.3 gi_348540351, XP_003457651.1, PREDICTED: L-amino-acid oxidase-like [Oreochromis niloticus] 83 2.70E-68 246 173 656902 2.2E-05 1.3 gi_348527490, XP_003451252.1, PREDICTED: egl nine homolog 3-like [Oreochromis niloticus] 86 1.54E-126 388 174 125328 1.4E-02 1.3 gi_348523247, XP_003449135.1, PREDICTED: interferon-induced protein 44-like [Oreochromis niloticus] 81 < 1.0E-180 611 175 151812 6.1E-07 1.3 gi_348519900, XP_003447467.1, PREDICTED: polypeptide N-acetylgalactosaminyltransferase 5-like 76 < 1.0E-180 809 [Oreochromis niloticus] 176 22710 4.0E-05 1.3 gi_348517542, XP_003446292.1, PREDICTED: calpain-1 catalytic subunit-like [Oreochromis niloticus] 76 < 1.0E-180 881 177 623711 1.3E-02 1.3 gi_260789262, XP_002589666.1, hypothetical protein BRAFLDRAFT_64030 [Branchiostoma floridae] 80 1.83E-21 99 >gi_229274847, EEN45677.1, hypothetical protein BRAFLDRAFT_64030 [Branchiostoma floridae] 178 513567 2.3E-04 1.3 gi_213513996, NP_001134191.1, Methyltransferase-like protein 6 [Salmo salar] >gi_209731338, 89 < 1.0E-180 582 ACI66538.1, Methyltransferase-like protein 6 [Salmo salar] 179 101018 1.4E-02 1.3 gi_171916109, NP_001116443.1, myxovirus (influenza virus) resistance G [Danio rerio] >gi_48427584, 60 1.96E-71 250 CAD67761.2, MxG protein [Danio rerio] 180 593505 8.1E-06 1.3 gi_165972455, NP_001107059.1, hydroxylysine kinase [Danio rerio] >gi_170652820, A8WFT6.1, 90 1.61E-65 237 AGPD1_DANRE RecName: Full=Hydroxylysine kinase; Short=5-hydroxy-L-lysine kinase >gi_159155391, AAI54452.1, Zgc:171550 protein [Danio rerio] >gi_213625913, AAI71595.1, Zgc:171550 [Danio rerio] >gi_213625925, AAI71623.1, Zgc:171550 [Danio rerio]

181 10284 1.5E-06 1.2 gi_410897635, XP_003962304.1, PREDICTED: zinc finger protein 36, C3H1 type-like 1-like [Takifugu 78 1.36E-138 442 rubripes] 182 156809 4.5E-03 1.2 gi_348541555, XP_003458252.1, PREDICTED: WASP homolog-associated protein with actin, 68 5.16E-07 62 membranes and microtubules-like [Oreochromis niloticus] 183 642610 < 1.0E-15 1.2 gi_348539718, XP_003457336.1, PREDICTED: forkhead box protein Q1-like [Oreochromis niloticus] 78 5.53E-90 294 184 10094 4.0E-02 1.2 gi_348524879, XP_003449950.1, PREDICTED: LOW QUALITY PROTEIN: protein lin-9 homolog 90 < 1.0E-180 816 [Oreochromis niloticus] 185 380731 3.1E-03 1.2 gi_326665123, XP_691524.5, PREDICTED: interferon-induced guanylate-binding protein 1 [Danio rerio] 56 6.62E-49 188 186 182405 5.1E-02 1.2 gi_317419739, CBN81775.1, Aryl hydrocarbon receptor repressor [Dicentrarchus labrax] 76 < 1.0E-180 809 187 124525 6.0E-03 1.2 gi_317419398, CBN81435.1, Centrosomal protein of 55 kDa [Dicentrarchus labrax] 75 8.77E-162 493 188 2430 1.9E-08 1.2 gi_292658798, NP_001167620.1, cytochrome P450, family 1, subfamily B, polypeptide 1 [Oncorhynchus 94 < 1.0E-180 970 mykiss] >gi_290576814, ADD51159.1, cytochrome P450 family 1 subfamily B polypeptide 1 [Oncorhynchus mykiss]

189 397389 < 1.0E-15 1.2 gi_291227093, XP_002733521.1, PREDICTED: RETRotransposon-like family member (retr-1)-like 64 1.23E-58 138 [Saccoglossus kowalevskii] 190 236220 5.4E-02 1.2 gi_225715262, ACO13477.1, Microfibril-associated glycoprotein 4 precursor [Esox lucius] 87 2.63E-131 389 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 191 716677 7.9E-07 1.2 gi_221219202, ACM08262.1, C-C motif chemokine 20 precursor [Salmo salar] 91 1.22E-40 148 192 27066 1.3E-04 1.2 gi_185133146, NP_001118086.1, decoy receptor 3 protein precursor [Oncorhynchus mykiss] 87 1.18E-180 516 >gi_14595071, CAC43329.1, putative decoy receptor 3 protein [Oncorhynchus mykiss] 193 301959 4.1E-02 1.2 gi_156385456, XP_001633646.1, predicted protein [Nematostella vectensis] >gi_156220719, 60 2.29E-16 84 EDO41583.1, predicted protein [Nematostella vectensis] 194 375043 9.4E-12 1.2 gi_156376616, XP_001630455.1, predicted protein [Nematostella vectensis] >gi_156217477, 56 6.17E-13 78 EDO38392.1, predicted protein [Nematostella vectensis] 195 430668 1.8E-09 1.2 gi_185133257, NM_001123683.1, Salmo salar Na/K/2Cl co-transporter (nkcc1a), mRNA >gi_114438953, 98.2 < 1.0E-180 4638 DQ864492.1, Salmo salar Na/K/2Cl co-transporter (NKCC1a) mRNA, complete cds 196 235278 1.4E-04 1.1 gi_47182883, CAG13563.1, unnamed protein product [Tetraodon nigroviridis] 68 6.08E-04 48 197 718648 4.6E-04 1.1 gi_410915276, XP_003971113.1, PREDICTED: chromosome-associated kinesin KIF4-like [Takifugu 74 3.13E-158 503 rubripes] 198 358759 1.0E-02 1.1 gi_356640273, NP_001239290.1, interferon regulatory factor 1 [Salmo salar] >gi_209734940, 97 7.69E-140 425 ACI68339.1, Interferon regulatory factor 1 [Salmo salar] 199 574605 1.4E-08 1.1 gi_348538822, XP_003456889.1, PREDICTED: hypothetical protein LOC100694373 [Oreochromis 67 7.69E-24 108 niloticus] 200 95238 2.1E-08 1.1 gi_348534685, XP_003454832.1, PREDICTED: leukotriene B4 receptor 1-like [Oreochromis niloticus] 75 3.02E-118 369 201 82087 1.1E-08 1.1 gi_348529374, XP_003452188.1, PREDICTED: structural maintenance of chromosomes protein 2-like 92 < 1.0E-180 1831 [Oreochromis niloticus] 202 760876 2.9E-06 1.1 gi_348523243, XP_003449133.1, PREDICTED: hypothetical protein LOC100710414 [Oreochromis 64 2.76E-104 339 niloticus] 203 539119 1.5E-06 1.1 gi_348520943, XP_003447986.1, PREDICTED: hypothetical protein LOC100692175 [Oreochromis 58 2.91E-69 255 niloticus] 204 405403 2.6E-03 1.1 gi_348511041, XP_003443053.1, PREDICTED: LOW QUALITY PROTEIN: helicase-like transcription 56 7.29E-34 148 factor-like [Oreochromis niloticus] 205 308910 2.0E-02 1.1 gi_327289712, XP_003229568.1, PREDICTED: zinc finger protein 658-like [Anolis carolinensis] 58 1.55E-130 409 206 124562 6.1E-06 1.1 gi_317419398, CBN81435.1, Centrosomal protein of 55 kDa [Dicentrarchus labrax] 75 3.22E-151 472 207 245009 2.7E-03 1.1 gi_295792356, ADG29182.1, prostate stem cell antigen precursor-like [Epinephelus coioides] 71 8.72E-64 206 208 614602 3.6E-04 1.1 gi_259155347, NP_001158735.1, pituitary tumor-transforming 1 [Salmo salar] >gi_223646204, 93 1.04E-93 285 ACN09860.1, Securin [Salmo salar] >gi_223672051, ACN12207.1, Securin [Salmo salar] 209 511841 4.7E-02 1.1 gi_226443143, NP_001140055.1, CD209 antigen-like protein A [Salmo salar] >gi_221221544, 66 1.73E-43 161 ACM09433.1, CD209 antigen-like protein A [Salmo salar] 210 16342 4.0E-10 1.1 gi_224048247, XP_002190236.1, PREDICTED: similar to nephrocan [Taeniopygia guttata] 63 2.05E-134 428 211 397361 2.1E-04 1.1 gi_213511424, NP_001134825.1, Cytosolic sulfotransferase 3 [Salmo salar] >gi_209736374, 98 < 1.0E-180 612 ACI69056.1, Cytosolic sulfotransferase 3 [Salmo salar] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 212 714325 9.8E-08 1.1 gi_213511184, NP_001134312.1, serine/threonine-protein kinase VRK1 [Salmo salar] >gi_209732302, 96 < 1.0E-180 362 ACI67020.1, Serine/threonine-protein kinase VRK1 [Salmo salar] 213 275404 5.3E-02 1.1 gi_213510760, NP_001134428.1, PHD finger protein 21A [Salmo salar] >gi_209733198, ACI67468.1, 85 1.51E-08 63 PHD finger protein 21A [Salmo salar] 214 318117 < 1.0E-15 1.1 gi_209736978, ACI69358.1, CD83 antigen precursor [Salmo salar] 91 1.04E-122 377 215 122647 1.5E-02 1.1 gi_18858689, NP_571374.1, hepatocyte nuclear factor 3-gamma [Danio rerio] >gi_2982341, 88 < 1.0E-180 621 AAC06362.1, fork head domain protein FKD2 [Danio rerio] 216 237571 1.1E-02 1.1 gi_18858601, NP_571323.1, early growth response protein 1 [Danio rerio] >gi_1352361, P26632.2, 72 1.32E-175 533 EGR1_DANRE RecName: Full=Early growth response protein 1; Short=EGR-1; AltName: Full=Zinc finger protein Krox-24 >gi_531456, AAA63651.1, egr1 [Danio rerio] >gi_190337755, AAI63873.1, Early growth response 1 [Danio rerio] >gi_1091895, prf, , 2022191A Zn finger protein

217 54067 2.9E-02 1.0 gi_47220850, CAG00057.1, unnamed protein product [Tetraodon nigroviridis] 74 1.05E-133 444 218 554481 5.3E-08 1.0 gi_410914403, XP_003970677.1, PREDICTED: aurora kinase B-like [Takifugu rubripes] 87 < 1.0E-180 546 219 305089 1.1E-10 1.0 gi_410913525, XP_003970239.1, PREDICTED: glycerophosphoinositol inositolphosphodiesterase 74 1.80E-144 472 GDPD2-like [Takifugu rubripes] 220 612733 8.6E-10 1.0 gi_410908433, XP_003967695.1, PREDICTED: neuroepithelial cell-transforming gene 1 protein-like 82 < 1.0E-180 821 [Takifugu rubripes] 221 714371 2.9E-05 1.0 gi_404250106, AFR53956.1, transposase [Cloning vector pT2/Gene-Trap] 91 2.00E-15 84 222 172669 1.8E-03 1.0 gi_348544077, XP_003459508.1, PREDICTED: glucagon receptor-like [Oreochromis niloticus] 86 < 1.0E-180 611 223 183578 6.0E-06 1.0 gi_348543245, XP_003459094.1, PREDICTED: hypothetical protein LOC100708308 [Oreochromis 66 5.34E-122 391 niloticus] 224 680389 6.1E-03 1.0 gi_348530406, XP_003452702.1, PREDICTED: leucine zipper putative tumor suppressor 1-like 68 1.05E-145 466 [Oreochromis niloticus] 225 305078 2.1E-11 1.0 gi_348515375, XP_003445215.1, PREDICTED: glycerophosphodiester phosphodiesterase domain- 68 6.28E-122 395 containing protein 5-like [Oreochromis niloticus] 226 78906 4.1E-06 1.0 gi_348041275, NP_001011729.2, transforming, acidic coiled-coil containing protein 3 [Takifugu rubripes] 55 1.14E-165 532 227 138440 8.0E-03 1.0 gi_326681346, XP_003201796.1, PREDICTED: hypothetical protein LOC100534786, partial [Danio rerio] 50 3.82E-50 196 228 235212 2.1E-05 1.0 gi_318065022, ADV36649.1, CD200 [Oncorhynchus mykiss] 84 7.34E-86 275 229 112486 1.1E-06 1.0 gi_317418583, CBN80621.1, Kinetochore protein Nuf2 [Dicentrarchus labrax] 83 < 1.0E-180 566 230 658241 1.6E-02 1.0 gi_226443222, NP_001140088.1, Centromere protein M [Salmo salar] >gi_221221914, ACM09618.1, 98 1.31E-118 350 Centromere protein M [Salmo salar] 231 552008 7.8E-06 1.0 gi_225716966, ACO14329.1, G1/S-specific cyclin-D2 [Esox lucius] 95 4.76E-163 510 232 613629 6.2E-03 1.0 gi_225707360, ACO09526.1, Kinetochore protein Spc25 [Osmerus mordax] 79 7.16E-93 299 233 212635 < 1.0E-15 1.0 gi_223648372, ACN10944.1, Transposable element Tcb2 transposase [Salmo salar] 91 7.19E-32 117 234 156734 < 1.0E-15 1.0 gi_221220962, ACM09142.1, CD9 antigen [Salmo salar] 92 2.41E-137 422 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 235 52501 3.2E-09 1.0 gi_213515118, NP_001135110.1, Serine/threonine-protein kinase 6 [Salmo salar] >gi_209154810, 97 < 1.0E-180 776 ACI33637.1, Serine/threonine-protein kinase 6 [Salmo salar] 236 54828 2.3E-02 1.0 gi_213514788, NP_001134708.1, Tumor necrosis factor receptor superfamily member 5 precursor 95 4.61E-129 394 [Salmo salar] >gi_209735360, ACI68549.1, Tumor necrosis factor receptor superfamily member 5 precursor [Salmo salar] 237 48015 1.2E-06 1.0 gi_213514228, NP_001133425.1, Retinoblastoma-binding protein 6 [Salmo salar] >gi_209153962, 100 3.39E-65 223 ACI33213.1, Retinoblastoma-binding protein 6 [Salmo salar] 238 143178 2.7E-03 1.0 gi_213513922, NP_001133906.1, G1/S-specific cyclin-E2 [Salmo salar] >gi_209155770, ACI34117.1, 96 < 1.0E-180 761 G1/S-specific cyclin-E2 [Salmo salar] 239 679 1.1E-06 1.0 gi_213512749, NP_001133446.1, Jeltraxin precursor [Salmo salar] >gi_209154038, ACI33251.1, 84 1.07E-88 300 Jeltraxin precursor [Salmo salar] 240 251216 1.9E-02 1.0 gi_213512555, NP_001134610.1, Phosphatidylcholine transfer protein [Salmo salar] >gi_209734630, 99 2.73E-143 421 ACI68184.1, Phosphatidylcholine transfer protein [Salmo salar] 241 511478 9.6E-03 1.0 gi_213511642, NP_001133495.1, Ribonucleoside-diphosphate reductase subunit M2 [Salmo salar] 99 < 1.0E-180 673 >gi_209154228, ACI33346.1, Ribonucleoside-diphosphate reductase subunit M2 [Salmo salar] 242 387478 1.0E-06 1.0 gi_209735458, ACI68598.1, G2/mitotic-specific cyclin-B1 [Salmo salar] 98 < 1.0E-180 780 243 642527 3.9E-02 1.0 gi_209734464, ACI68101.1, C-C motif chemokine 28 precursor [Salmo salar] >gi_209735076, 95 2.02E-30 117 ACI68407.1, C-C motif chemokine 28 precursor [Salmo salar] >gi_209738088, ACI69913.1, C-C motif chemokine 28 precursor [Salmo salar]

244 387503 < 1.0E-15 1.0 gi_209730442, ACI66090.1, G2/mitotic-specific cyclin-B1 [Salmo salar] 98 < 1.0E-180 767 245 756943 3.6E-02 1.0 gi_209156102, ACI34283.1, Interferon-induced protein with tetratricopeptide repeats 5 [Salmo salar] 83 < 1.0E-180 713 246 731846 9.4E-05 1.0 gi_185132957, NP_001117006.1, cystic fibrosis transmembrane conductance regulator II [Salmo salar] 98 < 1.0E-180 2931 >gi_7188560, AAF37801.1, AF161070_1 cystic fibrosis transmembrane conductance regulator II [Salmo salar] 247 655182 2.9E-02 1.0 gi_185132685, NP_001117887.1, putative interferon-alpha/beta receptor alpha chain precursor 44 8.04E-11 71 [Oncorhynchus mykiss] >gi_121487407, CAG25517.1, putative interferon-alpha/beta receptor alpha chain [Oncorhynchus mykiss]

248 18414 1.1E-02 1.0 gi_169640220, DAA06156.1, TPA_exp: claudin 10e [Salmo salar] 100 2.06E-130 392 249 5246 1.0E-02 1.0 gi_148725400, CAK10979.2, novel protein similar to vertebrate anillin, actin binding protein (scraps 90 1.59E-70 255 homolog, Drosophila) (ANLN) [Danio rerio] 250 556671 1.8E-02 1.0 gi_119512519, ZP_01631598.1, Arginyl tRNA synthetase, anticodon binding [Nodularia spumigena 57 8.64E-25 108 CCY9414] >gi_119462826, EAW43784.1, Arginyl tRNA synthetase, anticodon binding [Nodularia spumigena CCY9414] 251 98378 6.7E-03 0.9 gi_94733955, CAK04352.1, novel protein similar to vertebrate G protein-coupled receptor 21 (GPR21) 90 3.49E-55 208 [Danio rerio] 252 225027 2.7E-02 0.9 gi_76163052, AAX30817.2, SJCHGC07841 protein [Schistosoma japonicum] 85 2.11E-10 67 253 390874 2.8E-02 0.9 gi_76153153, AAX24812.2, SJCHGC04011 protein [Schistosoma japonicum] 77 2.88E-07 54 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 254 131826 1.1E-02 0.9 gi_76153153, AAX24812.2, SJCHGC04011 protein [Schistosoma japonicum] 70 9.13E-07 59 255 641699 4.9E-02 0.9 gi_72384337, NP_001026841.1, Golgi transport 1 homolog B [Danio rerio] >gi_66910526, AAH97251.1, 94 1.35E-35 142 Zgc:114204 [Danio rerio] 256 378082 7.6E-07 0.9 gi_47229109, CAG03861.1, unnamed protein product [Tetraodon nigroviridis] 79 7.96E-142 465 257 556042 1.3E-03 0.9 gi_410927255, XP_003977065.1, PREDICTED: uncharacterized protein LOC101071083 [Takifugu 72 1.58E-09 70 rubripes] 258 661021 2.9E-02 0.9 gi_410926427, XP_003976680.1, PREDICTED: cytoskeleton-associated protein 5-like isoform 3 78 < 1.0E-180 1620 [Takifugu rubripes] 259 14690 2.2E-03 0.9 gi_410925363, XP_003976150.1, PREDICTED: phosphatidylinositol 5-phosphate 4-kinase type-2 91 < 1.0E-180 626 gamma-like isoform 1 [Takifugu rubripes] 260 583419 5.9E-04 0.9 gi_410925174, XP_003976056.1, PREDICTED: interphotoreceptor matrix proteoglycan 1-like [Takifugu 71 5.51E-19 94 rubripes] 261 651810 7.6E-03 0.9 gi_410917956, XP_003972452.1, PREDICTED: forkhead box protein E1-like [Takifugu rubripes] 74 5.04E-127 392 262 351926 8.7E-03 0.9 gi_41053453, NP_956891.1, ephrin A1a precursor [Danio rerio] >gi_34783755, AAH56770.1, Ephrin 87 2.28E-109 350 A1a [Danio rerio] >gi_94732426, CAK05183.1, novel protein (zgc:63493) [Danio rerio] 263 368398 4.2E-02 0.9 gi_387155683, CBL94866.1, IL-10 receptor-1 precursor [Oncorhynchus mykiss] 91 < 1.0E-180 1023 264 579670 5.6E-04 0.9 gi_348538738, XP_003456847.1, PREDICTED: mitochondrial ornithine transporter 1-like [Oreochromis 90 1.36E-171 507 niloticus] 265 33226 1.8E-02 0.9 gi_348538509, XP_003456733.1, PREDICTED: lymphoid-specific helicase [Oreochromis niloticus] 88 < 1.0E-180 1225 266 607759 3.6E-13 0.9 gi_348537292, XP_003456129.1, PREDICTED: aquaporin-3-like [Oreochromis niloticus] 91 4.41E-175 501 267 408116 2.6E-02 0.9 gi_348528332, XP_003451672.1, PREDICTED: N-lysine methyltransferase SETD8-A-like [Oreochromis 69 3.40E-101 334 niloticus] 268 494521 1.8E-02 0.9 gi_348518403, XP_003446721.1, PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1- 60 < 1.0E-180 920 like [Oreochromis niloticus] 269 101490 6.9E-04 0.9 gi_348516814, XP_003445932.1, PREDICTED: hypothetical protein LOC100696796 [Oreochromis 71 6.23E-23 101 niloticus] 270 19803 4.3E-03 0.9 gi_348513470, XP_003444265.1, PREDICTED: tumor protein 63-like isoform 1 [Oreochromis niloticus] 91 < 1.0E-180 932 271 174641 7.5E-03 0.9 gi_348505645, XP_003440371.1, PREDICTED: protein zwilch homolog [Oreochromis niloticus] 89 < 1.0E-180 727 272 308951 2.0E-02 0.9 gi_334313554, XP_003339926.1, PREDICTED: zinc finger protein 709-like [Monodelphis domestica] 58 1.58E-74 262 273 469662 5.5E-05 0.9 gi_326674386, XP_001338900.4, PREDICTED: hypothetical protein LOC798456 [Danio rerio] 63 3.27E-179 610 274 583446 3.0E-02 0.9 gi_326668956, XP_001920764.3, PREDICTED: hypothetical protein LOC100000002 [Danio rerio] 53 2.45E-31 143 275 571349 2.5E-04 0.9 gi_326665164, XP_003197983.1, PREDICTED: PAS domain-containing serine/threonine-protein kinase 59 < 1.0E-180 737 [Danio rerio] >gi_169154325, CAQ13600.1, novel protein similar to vertebrate PAS domain containing serine/threonine kinase (PASK) [Danio rerio]

276 235450 7.9E-03 0.9 gi_318065022, ADV36649.1, CD200 [Oncorhynchus mykiss] 92 1.76E-90 292 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 277 750841 4.2E-05 0.9 gi_317419847, CBN81883.1, Pyruvate dehydrogenase [Dicentrarchus labrax] 95 < 1.0E-180 771 278 244832 6.7E-03 0.9 gi_295792356, ADG29182.1, prostate stem cell antigen precursor-like [Epinephelus coioides] 66 3.35E-19 92 279 332653 1.3E-02 0.9 gi_292620944, XP_696722.2, PREDICTED: nucleotide exchange factor SIL1-like [Danio rerio] 78 < 1.0E-180 560 280 569630 1.1E-03 0.9 gi_229365746, ACQ57853.1, Meiotic nuclear division protein 1 homolog [Anoplopoma fimbria] 94 1.69E-117 356 281 685487 2.6E-03 0.9 gi_226443300, NP_001139845.1, Vesicle transport protein SFT2B [Salmo salar] >gi_221219288, 100 5.08E-73 230 ACM08305.1, Vesicle transport protein SFT2B [Salmo salar] 282 301551 2.0E-07 0.9 gi_225706062, ACO08877.1, Tumor necrosis factor receptor superfamily member 14 precursor [Osmerus 67 1.05E-65 236 mordax] 283 53666 1.7E-03 0.9 gi_223646840, ACN10178.1, Thymidylate synthase [Salmo salar] >gi_223672701, ACN12532.1, 85 < 1.0E-180 639 Thymidylate synthase [Salmo salar] 284 302652 9.3E-05 0.9 gi_213627492, AAI71448.1, Zgc:86764 [Danio rerio] 77 < 1.0E-180 640 285 398869 2.4E-04 0.9 gi_213513316, NP_001135012.1, DNA replication complex GINS protein SLD5 [Salmo salar] 95 1.32E-128 384 >gi_209738002, ACI69870.1, DNA replication complex GINS protein SLD5 [Salmo salar] >gi_223646946, ACN10231.1, DNA replication complex GINS protein SLD5 [Salmo salar] >gi_223672809, ACN12586.1, DNA replication complex GINS protein SLD5 [Salmo salar] >gi_303660173, ADM15983.1, DNA replication complex GINS protein SLD5 [Salmo salar]

286 649834 8.2E-11 0.9 gi_213512634, NP_001133671.1, cyclin-A2 [Salmo salar] >gi_209154884, ACI33674.1, Cyclin-A2 98 5.89E-156 461 [Salmo salar] 287 552508 1.1E-02 0.9 gi_213512127, NP_001134803.1, Lysosomal thioesterase PPT2-A precursor [Salmo salar] 99 2.82E-142 424 >gi_209736182, ACI68960.1, Lysosomal thioesterase PPT2-A precursor [Salmo salar] 288 511462 5.0E-02 0.9 gi_213511642, NP_001133495.1, Ribonucleoside-diphosphate reductase subunit M2 [Salmo salar] 96 < 1.0E-180 732 >gi_209154228, ACI33346.1, Ribonucleoside-diphosphate reductase subunit M2 [Salmo salar] 289 560891 2.0E-02 0.9 gi_209732956, ACI67347.1, Nicotinamide riboside kinase 2 [Salmo salar] >gi_209735822, ACI68780.1, 98 1.22E-92 297 Nicotinamide riboside kinase 2 [Salmo salar] 290 17441 2.3E-02 0.9 gi_189517039, XP_001340467.2, PREDICTED: hypothetical protein LOC100000219 [Danio rerio] 51 1.27E-86 174 291 237538 5.3E-02 0.9 gi_18858601, NP_571323.1, early growth response protein 1 [Danio rerio] >gi_1352361, P26632.2, 79 < 1.0E-180 597 EGR1_DANRE RecName: Full=Early growth response protein 1; Short=EGR-1; AltName: Full=Zinc finger protein Krox-24 >gi_531456, AAA63651.1, egr1 [Danio rerio] >gi_190337755, AAI63873.1, Early growth response 1 [Danio rerio] >gi_1091895, prf, , 2022191A Zn finger protein

292 237552 3.8E-07 0.9 gi_18858601, NP_571323.1, early growth response protein 1 [Danio rerio] >gi_1352361, P26632.2, 84 < 1.0E-180 615 EGR1_DANRE RecName: Full=Early growth response protein 1; Short=EGR-1; AltName: Full=Zinc finger protein Krox-24 >gi_531456, AAA63651.1, egr1 [Danio rerio] >gi_190337755, AAI63873.1, Early growth response 1 [Danio rerio] >gi_1091895, prf, , 2022191A Zn finger protein

293 723356 3.9E-02 0.9 gi_185134411, NP_001117919.1, CCAAT/enhancer-binding protein beta [Oncorhynchus mykiss] 96 2.29E-174 508 >gi_33304538, AAN41660.1, CCAAT/enhancer-binding protein beta [Oncorhynchus mykiss] >gi_33439492, AAQ18795.1, CCAAT/enhancer binding protein beta [Oncorhynchus mykiss] >gi_225705818, ACO08755.1, CCAAT/enhancer-binding protein beta [Oncorhynchus mykiss] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 294 472622 3.2E-03 0.9 gi_185134290, NP_001117918.1, glycogen synthase kinase binding protein [Oncorhynchus mykiss] 89 3.49E-16 57 >gi_37544637, AAM44075.1, guanylate-binding protein [Oncorhynchus mykiss] 295 145767 3.2E-14 0.9 gi_185133520, NP_001118097.1, c-x-c chemokine receptor type 3A isoform 1 [Oncorhynchus mykiss] 95 < 1.0E-180 655 >gi_133921937, CAI61936.1, c-x-c chemokine receptor type 3A, variant 1 [Oncorhynchus mykiss] 296 559584 3.4E-06 0.9 gi_185132138, NP_001117797.1, sodium bicarbonate cotransporter [Oncorhynchus mykiss] 96 < 1.0E-180 1935 >gi_24266569, AAN52239.1, AF434166_1 sodium bicarbonate cotransporter [Oncorhynchus mykiss] 297 663789 5.3E-02 0.9 gi_156372888, XP_001629267.1, predicted protein [Nematostella vectensis] >gi_156216263, 36 5.60E-05 48 EDO37204.1, predicted protein [Nematostella vectensis] 298 415817 1.2E-05 0.9 gi_125820434, XP_001333205.1, PREDICTED: hypothetical protein LOC796178 [Danio rerio] 61 7.90E-17 83 299 466375 1.0E-06 0.9 gi_125807411, XP_001343562.1, PREDICTED: hypothetical protein LOC100004199 [Danio rerio] 50 1.30E-55 215 300 305247 3.2E-10 0.9 gi_112363422, ABI16036.1, leukocyte immune-type receptor TS32.15 L1.1a, partial [Ictalurus punctatus] 65 5.24E-128 413 301 654392 8.0E-05 0.8 gi_54792766, NP_001006662.1, kinetochore-associated protein KNL-2 homolog [Danio rerio] 49 9.84E-85 309 >gi_26984618, CAD32865.2, novel protein similar to human hypothetical protein FLJ11186 [Danio rerio] 302 339430 6.1E-05 0.8 gi_47216412, CAG01963.1, unnamed protein product [Tetraodon nigroviridis] 84 < 1.0E-180 1653 303 86065 4.8E-03 0.8 gi_410914403, XP_003970677.1, PREDICTED: aurora kinase B-like [Takifugu rubripes] 86 < 1.0E-180 543 304 729912 5.5E-06 0.8 gi_410898299, XP_003962635.1, PREDICTED: phospholipase D4-like [Takifugu rubripes] 79 < 1.0E-180 636 305 9671 2.5E-02 0.8 gi_348544115, XP_003459527.1, PREDICTED: histone-lysine N-methyltransferase EZH2-like 85 < 1.0E-180 886 [Oreochromis niloticus] 306 604513 2.3E-04 0.8 gi_348542989, XP_003458966.1, PREDICTED: transcription factor Sox-19a-like [Oreochromis niloticus] 79 2.59E-113 366 307 212377 3.3E-02 0.8 gi_348540943, XP_003457946.1, PREDICTED: Schwann cell myelin protein-like [Oreochromis niloticus] 64 1.18E-58 211 308 33247 1.3E-03 0.8 gi_348538509, XP_003456733.1, PREDICTED: lymphoid-specific helicase [Oreochromis niloticus] 87 < 1.0E-180 1237 309 643891 1.4E-02 0.8 gi_348536122, XP_003455546.1, PREDICTED: DNA excision repair protein ERCC-6-like [Oreochromis 70 < 1.0E-180 1201 niloticus] 310 80589 4.9E-03 0.8 gi_348531152, XP_003453074.1, PREDICTED: lamin-B receptor-like [Oreochromis niloticus] 80 < 1.0E-180 716 311 316726 1.1E-03 0.8 gi_348531144, XP_003453070.1, PREDICTED: probable ribonuclease ZC3H12D-like [Oreochromis 65 < 1.0E-180 610 niloticus] 312 506982 1.6E-04 0.8 gi_348522279, XP_003448653.1, PREDICTED: condensin complex subunit 2-like [Oreochromis niloticus] 83 < 1.0E-180 675 313 618761 1.4E-03 0.8 gi_348520692, XP_003447861.1, PREDICTED: rho GTPase-activating protein 11A-like [Oreochromis 85 4.04E-139 466 niloticus] 314 494430 4.2E-02 0.8 gi_348518403, XP_003446721.1, PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1- 59 < 1.0E-180 1080 like [Oreochromis niloticus] 315 676422 2.2E-02 0.8 gi_348517829, XP_003446435.1, PREDICTED: retinoid-inducible serine carboxypeptidase-like 90 < 1.0E-180 569 [Oreochromis niloticus] 316 328179 9.2E-05 0.8 gi_348517221, XP_003446133.1, PREDICTED: UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose 90 6.29E-146 466 transporter-like [Oreochromis niloticus] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 317 27979 2.3E-02 0.8 gi_348515341, XP_003445198.1, PREDICTED: centromere protein I-like [Oreochromis niloticus] 84 < 1.0E-180 1001 318 706595 5.4E-07 0.8 gi_348511191, XP_003443128.1, PREDICTED: protein KRI1 homolog [Oreochromis niloticus] 79 < 1.0E-180 742 319 543418 5.3E-03 0.8 gi_348506269, XP_003440682.1, PREDICTED: rho-related GTP-binding protein RhoV-like [Oreochromis 89 9.55E-116 361 niloticus] 320 30597 1.5E-05 0.8 gi_348500820, XP_003437970.1, PREDICTED: kinesin-like protein KIF2C-like [Oreochromis niloticus] 87 < 1.0E-180 604 321 318109 4.4E-02 0.8 gi_33090227, AAP93909.1, CD83 [Oncorhynchus mykiss] >gi_33090231, AAP93911.1, CD83 90 1.96E-121 368 [Oncorhynchus mykiss] 322 406061 2.8E-02 0.8 gi_326675469, XP_003200362.1, PREDICTED: keratinocyte-associated protein 3-like [Danio rerio] 83 4.69E-95 314 323 649678 2.6E-05 0.8 gi_326674386, XP_001338900.4, PREDICTED: hypothetical protein LOC798456 [Danio rerio] 55 7.24E-127 458 324 636329 4.3E-02 0.8 gi_326673470, XP_003199893.1, PREDICTED: ES1 protein homolog, mitochondrial isoform 1 [Danio 87 5.62E-63 204 rerio] >gi_326673472, XP_003199894.1, PREDICTED: ES1 protein homolog, mitochondrial isoform 2 [Danio rerio] 325 583530 < 1.0E-15 0.8 gi_326668956, XP_001920764.3, PREDICTED: hypothetical protein LOC100000002 [Danio rerio] 45 4.02E-25 72 326 60035 3.1E-03 0.8 gi_320461730, NP_001189366.1, spindle and kinetochore-associated protein 3 [Danio rerio] 58 2.47E-95 318 327 235211 5.6E-06 0.8 gi_318065022, ADV36649.1, CD200 [Oncorhynchus mykiss] 88 5.78E-92 295 328 542697 3.2E-02 0.8 gi_292611339, XP_699777.3, PREDICTED: GTPase IMAP family member 4-like [Danio rerio] 66 1.94E-60 221 329 27723 5.9E-05 0.8 gi_291190745, NP_001167050.1, Condensin complex subunit 3 [Salmo salar] >gi_223647856, 93 < 1.0E-180 1541 ACN10686.1, Condensin complex subunit 3 [Salmo salar] 330 109031 5.0E-02 0.8 gi_268556760, XP_002636369.1, Hypothetical protein CBG08669 [Caenorhabditis briggsae] 60 1.68E-30 129 331 137122 5.1E-02 0.8 gi_259155148, NP_001158816.1, S-adenosylmethionine decarboxylase proenzyme [Salmo salar] 99 < 1.0E-180 656 >gi_223647538, ACN10527.1, S-adenosylmethionine decarboxylase proenzyme [Salmo salar] 332 321540 2.1E-02 0.8 gi_225716632, ACO14162.1, Serine protease 27 precursor [Esox lucius] 85 1.66E-120 394 333 84551 2.6E-03 0.8 gi_225715702, ACO13697.1, Forkhead box protein Q1 [Esox lucius] 88 2.02E-163 478 334 258565 1.3E-07 0.8 gi_225715202, ACO13447.1, Cornifelin homolog B [Esox lucius] 82 3.34E-39 154 335 350009 1.4E-03 0.8 gi_223648068, ACN10792.1, Neutrophil cytosol factor 2 [Salmo salar] 98 < 1.0E-180 793 336 273919 4.2E-09 0.8 gi_221222226, ACM09774.1, Zymogen granule membrane protein 16 precursor [Salmo salar] 48 6.28E-13 78 337 664866 2.4E-03 0.8 gi_221219544, ACM08433.1, Cyclin-dependent kinases regulatory subunit 1 [Salmo salar] 100 1.00E-47 165 >gi_221219678, ACM08500.1, Cyclin-dependent kinases regulatory subunit 1 [Salmo salar] >gi_221221464, ACM09393.1, Cyclin-dependent kinases regulatory subunit 1 [Salmo salar]

338 717094 2.6E-02 0.8 gi_220679133, CAX12457.1, novel protein similar to vetebrate diacylglycerol kinase, delta 130kDa 89 < 1.0E-180 1404 (DGKD) [Danio rerio] 339 113900 4.4E-02 0.8 gi_213626618, AAI69729.1, BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) 52 4.75E-153 495 [Xenopus laevis] 340 329552 1.9E-02 0.8 gi_213515046, NP_001133657.1, CDC45-related protein [Salmo salar] >gi_209154838, ACI33651.1, 99 < 1.0E-180 1052 CDC45-related protein [Salmo salar] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 341 323082 9.7E-06 0.8 gi_213513042, NP_001134270.1, fucosyltransferase 9 [Salmo salar] >gi_209731992, ACI66865.1, 96 < 1.0E-180 736 Alpha-1,3-fucosyltransferase [Salmo salar] 342 600444 6.0E-03 0.8 gi_213512967, NP_001133246.1, Epidermis-type lipoxygenase 3 [Salmo salar] >gi_209147549, 97 < 1.0E-180 888 ACI32895.1, Epidermis-type lipoxygenase 3 [Salmo salar] 343 649829 1.5E-02 0.8 gi_213512634, NP_001133671.1, cyclin-A2 [Salmo salar] >gi_209154884, ACI33674.1, Cyclin-A2 95 < 1.0E-180 448 [Salmo salar] 344 284621 1.2E-02 0.8 gi_213511354, NP_001135192.1, p53 apoptosis effector related to PMP-22 [Salmo salar] 100 1.83E-40 159 >gi_209151977, ACI33091.1, p53 apoptosis effector related to PMP-22 [Salmo salar] 345 112886 1.6E-02 0.8 gi_209731862, ACI66800.1, TNF receptor-associated factor 5 [Salmo salar] 95 3.19E-178 540 346 653664 3.4E-02 0.8 gi_209730618, ACI66178.1, Baculoviral IAP repeat-containing protein 5 [Salmo salar] 99 3.16E-90 273 347 609529 1.7E-02 0.8 gi_169146068, CAQ13980.1, novel protein similar to vertebrate membrane associated guanylate kinase, 82 1.68E-147 494 WW and PDZ domain containing 1 (MAGI1) [Danio rerio] 348 685969 3.2E-02 0.8 gi_165993227, CAP71925.1, si:dkey-81p7.13 [Danio rerio] 53 3.63E-52 200 349 664741 7.3E-05 0.7 gi_49227597, NP_001001838.1, DNA topoisomerase I, mitochondrial precursor [Danio rerio] 82 < 1.0E-180 832 >gi_40841767, AAR92364.1, mitochondrial topoisomerase I [Danio rerio] >gi_67678162, AAH96851.1, Mitochondrial topoisomerase I [Danio rerio]

350 656974 7.3E-10 0.7 gi_47212871, CAF93228.1, unnamed protein product [Tetraodon nigroviridis] 58 4.51E-09 67 351 25531 3.6E-02 0.7 gi_47207707, CAF95282.1, unnamed protein product [Tetraodon nigroviridis] 68 < 1.0E-180 850 352 196366 7.7E-04 0.7 gi_410928632, XP_003977704.1, PREDICTED: desmoglein-2-like [Takifugu rubripes] 65 < 1.0E-180 824 353 328766 4.4E-02 0.7 gi_410923869, XP_003975404.1, PREDICTED: poly(U)-specific endoribonuclease-C-like [Takifugu 91 6.41E-120 292 rubripes] 354 710712 1.9E-04 0.7 gi_410914104, XP_003970528.1, PREDICTED: protocadherin-20-like [Takifugu rubripes] 71 < 1.0E-180 908 355 402633 1.1E-03 0.7 gi_407263762, XP_003945527.1, PREDICTED: zinc finger protein 709-like, partial [Mus musculus] 58 1.79E-137 455 356 583788 9.9E-04 0.7 gi_395515531, XP_003761956.1, PREDICTED: protein NLRC3 [Sarcophilus harrisii] 46 1.57E-57 228 357 127249 4.4E-03 0.7 gi_37780424, AAO64438.1, deoxycytidine kinase 2 [Danio rerio] >gi_63101770, AAH95077.1, 95 4.88E-165 487 Zgc:110540 protein [Danio rerio] >gi_157423304, AAI53550.1, Zgc:110540 [Danio rerio] >gi_213627444, AAI71341.1, Zgc:110540 [Danio rerio] >gi_213627446, AAI71343.1, Zgc:110540 [Danio rerio]

358 6889 4.9E-04 0.7 gi_354497867, XP_003511039.1, PREDICTED: integrin alpha-X [Cricetulus griseus] 57 1.34E-142 392 359 124024 1.0E-02 0.7 gi_348542989, XP_003458966.1, PREDICTED: transcription factor Sox-19a-like [Oreochromis niloticus] 86 1.08E-133 422 360 569007 6.3E-03 0.7 gi_348531288, XP_003453142.1, PREDICTED: kinesin-like protein KIF15-A-like [Oreochromis niloticus] 83 < 1.0E-180 1624 361 714804 3.8E-05 0.7 gi_348529904, XP_003452452.1, PREDICTED: RING finger protein B-like [Oreochromis niloticus] 78 < 1.0E-180 692 362 555368 3.3E-02 0.7 gi_348528468, XP_003451739.1, PREDICTED: general transcription factor IIH subunit 3-like 90 4.44E-162 476 [Oreochromis niloticus] 363 483283 7.1E-09 0.7 gi_348526377, XP_003450696.1, PREDICTED: hypothetical protein LOC100708366 [Oreochromis 59 4.53E-38 155 niloticus] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 364 168521 2.3E-05 0.7 gi_348525328, XP_003450174.1, PREDICTED: centromere protein X-like [Oreochromis niloticus] 82 4.11E-09 63 365 486965 1.6E-11 0.7 gi_348524434, XP_003449728.1, PREDICTED: hypothetical protein LOC100690846 [Oreochromis 62 1.12E-16 90 niloticus] 366 657247 1.6E-02 0.7 gi_348522295, XP_003448661.1, PREDICTED: hypothetical protein LOC100693622 [Oreochromis 47 4.00E-112 383 niloticus] 367 244894 1.7E-04 0.7 gi_348521448, XP_003448238.1, PREDICTED: chromobox protein homolog 5-like [Oreochromis 96 1.12E-43 169 niloticus] 368 728685 3.6E-02 0.7 gi_348520710, XP_003447870.1, PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 80 < 1.0E-180 587 beta-like [Oreochromis niloticus] 369 615441 1.6E-03 0.7 gi_348517050, XP_003446048.1, PREDICTED: tyrosine-protein phosphatase non-receptor type 7-like 77 1.52E-111 221 [Oreochromis niloticus] 370 72363 1.0E-05 0.7 gi_348513737, XP_003444398.1, PREDICTED: notch-regulated ankyrin repeat-containing protein A-like 98 1.21E-62 220 [Oreochromis niloticus] 371 689680 5.4E-02 0.7 gi_348503676, XP_003439390.1, PREDICTED: condensin-2 complex subunit G2-like [Oreochromis 78 < 1.0E-180 1229 niloticus] 372 152259 3.4E-03 0.7 gi_348501846, XP_003438480.1, PREDICTED: rhotekin-2-like [Oreochromis niloticus] 75 < 1.0E-180 760 373 78920 1.3E-03 0.7 gi_348041275, NP_001011729.2, transforming, acidic coiled-coil containing protein 3 [Takifugu rubripes] 58 < 1.0E-180 588 374 550697 1.4E-04 0.7 gi_344249965, EGW06069.1, Ankyrin repeat domain-containing protein 37 [Cricetulus griseus] 65 1.67E-18 93 375 492879 7.4E-06 0.7 gi_326674126, XP_003200076.1, PREDICTED: integrin alpha-E-like [Danio rerio] 62 < 1.0E-180 701 376 400336 3.6E-02 0.7 gi_326668941, XP_688146.5, PREDICTED: protein-glutamine gamma-glutamyltransferase 2-like [Danio 70 < 1.0E-180 704 rerio] 377 246269 3.8E-02 0.7 gi_326667463, XP_003198602.1, PREDICTED: NACHT, LRR and PYD domains-containing protein 3 61 < 1.0E-180 721 [Danio rerio] 378 322268 1.2E-02 0.7 gi_326666615, XP_690759.4, PREDICTED: plexin-C1 [Danio rerio] 70 < 1.0E-180 1085 379 26126 4.6E-02 0.7 gi_317420109, CBN82145.1, Microtubule-associated serine/threonine-protein kinase-like [Dicentrarchus 69 < 1.0E-180 978 labrax] 380 6039 7.1E-05 0.7 gi_292623243, XP_001332938.3, PREDICTED: grainyhead-like 3 [Danio rerio] 75 < 1.0E-180 666 381 552371 5.6E-03 0.7 gi_292618304, XP_697274.2, PREDICTED: cytoskeleton-associated protein 2 [Danio rerio] 57 6.36E-124 396 382 297903 1.1E-02 0.7 gi_291190494, NP_001167378.1, HGV2 protein [Salmo salar] >gi_223672951, ACN12657.1, HGV2 96 < 1.0E-180 792 [Salmo salar] 383 723354 4.2E-03 0.7 gi_289177112, NP_001165969.1, CCAAT/enhancer binding protein beta2 [Oncorhynchus mykiss] 96 2.02E-173 502 >gi_90019518, ABD84407.1, CCAAT/enhancer binding protein beta2 [Oncorhynchus mykiss] 384 277908 5.8E-03 0.7 gi_262409831, ACY66646.1, C-type lectin A [Oplegnathus fasciatus] 53 1.00E-47 183 385 724405 1.6E-03 0.7 gi_238231401, NP_001153973.1, peptidylarginine deiminase [Oncorhynchus mykiss] >gi_223645889, 98 < 1.0E-180 1319 CAX45844.1, peptidylarginine deiminase [Oncorhynchus mykiss] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 386 191896 2.6E-03 0.7 gi_225714882, ACO13287.1, Proliferating cell nuclear antigen [Esox lucius] 100 2.10E-172 504 387 583825 9.8E-03 0.7 gi_225706298, ACO08995.1, Replication factor C subunit 3 [Osmerus mordax] 97 < 1.0E-180 678 388 288702 6.0E-03 0.7 gi_225706062, ACO08877.1, Tumor necrosis factor receptor superfamily member 14 precursor [Osmerus 72 2.01E-60 216 mordax] 389 57168 3.2E-03 0.7 gi_224613412, ACN60285.1, SLIT-ROBO Rho GTPase-activating protein 1 [Salmo salar] 89 < 1.0E-180 871 390 234942 2.1E-06 0.7 gi_223647762, ACN10639.1, Tropomyosin alpha-3 chain [Salmo salar] 100 4.21E-64 231 391 406659 2.7E-02 0.7 gi_223647638, ACN10577.1, Epithelial cadherin precursor [Salmo salar] 80 < 1.0E-180 538 392 77932 2.4E-05 0.7 gi_223647638, ACN10577.1, Epithelial cadherin precursor [Salmo salar] 85 1.88E-125 407 393 406644 4.2E-02 0.7 gi_223647638, ACN10577.1, Epithelial cadherin precursor [Salmo salar] 89 < 1.0E-180 748 394 629967 1.1E-02 0.7 gi_223647006, ACN10261.1, Importin subunit alpha-2 [Salmo salar] >gi_223672871, ACN12617.1, 89 < 1.0E-180 945 Importin subunit alpha-2 [Salmo salar] 395 638546 2.4E-03 0.7 gi_221219742, ACM08532.1, Non-histone chromosomal protein H6 [Salmo salar] >gi_221219900, 100 1.28E-10 68 ACM08611.1, Non-histone chromosomal protein H6 [Salmo salar] >gi_221221472, ACM09397.1, Non- histone chromosomal protein H6 [Salmo salar] >gi_221222148, ACM09735.1, Non-histone chromosomal protein H6 [Salmo salar] >gi_221222336, ACM09829.1, Non-histone chromosomal protein H6 [Salmo salar] 396 219455 2.5E-04 0.7 gi_221130397, XP_002166794.1, PREDICTED: similar to protein-tyrosine phosphatase CRYPalpha1 66 2.97E-04 54 [Hydra magnipapillata] 397 231568 1.2E-02 0.7 gi_21489870, AAM55226.1, AF515834_1CA protein [Schistosoma japonicum] 97 2.52E-17 89 398 164130 1.9E-02 0.7 gi_213625187, AAI70022.1, Serine/threonine kinase 10 (polo-like kinase kinase 1) [Xenopus laevis] 66 6.92E-60 138 399 621154 1.3E-03 0.7 gi_213514942, NP_001134623.1, cell division cycle 2 [Salmo salar] >gi_209734764, ACI68251.1, Cell 96 7.93E-142 433 division control protein 2 homolog [Salmo salar] 400 59689 3.3E-04 0.7 gi_213514774, NP_001133768.1, G2/mitotic-specific cyclin-B3 [Salmo salar] >gi_209155268, 94 < 1.0E-180 639 ACI33866.1, G2/mitotic-specific cyclin-B3 [Salmo salar] 401 377469 3.7E-03 0.7 gi_213514692, NP_001133655.1, Eukaryotic translation initiation factor 2 subunit 1 [Salmo salar] 99 2.58E-168 517 >gi_209154830, ACI33647.1, Eukaryotic translation initiation factor 2 subunit 1 [Salmo salar] 402 728917 4.7E-02 0.7 gi_213512619, NP_001135257.1, Replication protein A 70 kDa DNA-binding subunit [Salmo salar] 97 < 1.0E-180 1208 >gi_209155456, ACI33960.1, Replication protein A 70 kDa DNA-binding subunit [Salmo salar] 403 2814 1.3E-02 0.7 gi_213511867, NP_001133353.1, RAS guanyl-releasing protein 2 [Salmo salar] >gi_209151714, 98 < 1.0E-180 1142 ACI33080.1, RAS guanyl-releasing protein 2 [Salmo salar] 404 410881 5.2E-04 0.7 gi_209734442, ACI68090.1, GTPase IMAP family member 7 [Salmo salar] 91 4.24E-61 215 405 194323 5.2E-02 0.7 gi_209154478, ACI33471.1, Probable histone deacetylase 1-B [Salmo salar] 82 4.73E-40 149 406 273956 4.1E-03 0.7 gi_198285501, ACH85289.1, hypothetical protein [Salmo salar] 85 1.34E-08 62 407 673010 2.4E-02 0.7 gi_185133577, NP_001117165.1, myxovirus resistance 1 [Salmo salar] >gi_1519384, AAB40994.1, Mx1 90 < 1.0E-180 844 protein [Salmo salar] >gi_197632229, ACH70838.1, myxovirus resistance 1 [Salmo salar] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 408 92891 6.2E-09 0.7 gi_169646833, NP_001112372.1, cysteinyl-tRNA synthetase, cytoplasmic [Danio rerio] 93 < 1.0E-180 1224 409 269500 3.0E-05 0.7 gi_125845888, XP_001335163.1, PREDICTED: caspase-8-like [Danio rerio] 64 1.54E-07 64 410 575713 2.2E-02 0.7 gi_125845863, XP_685228.2, PREDICTED: serine/threonine-protein kinase Nek1 [Danio rerio] 68 1.88E-58 221 411 673486 3.9E-05 0.7 gi_123704669, NP_001074055.1, niban-like protein 1 [Danio rerio] >gi_120537617, AAI29201.1, 75 < 1.0E-180 998 Zgc:158319 [Danio rerio] 412 353500 1.2E-02 0.6 gi_9937617, AAG02533.1, AF296387_1MHC class II beta-chain [Oncorhynchus mykiss] >gi_9937619, 76 9.36E-51 191 AAG02534.1, AF296388_1 MHC class II beta-chain [Oncorhynchus mykiss] 413 29582 1.8E-02 0.6 gi_56788875, AAH88598.1, lsp1-prov protein, partial [Xenopus (Silurana) tropicalis] 77 6.08E-48 182 414 339410 1.4E-07 0.6 gi_47216412, CAG01963.1, unnamed protein product [Tetraodon nigroviridis] 89 < 1.0E-180 920 415 286316 8.2E-03 0.6 gi_47086779, NP_997793.1, cytotoxic granule-associated RNA binding protein 1 [Danio rerio] 95 6.83E-160 505 >gi_28279551, AAH45368.1, Cytotoxic granule-associated RNA binding protein 1 [Danio rerio] >gi_148724923, CAM13160.2, novel protein similar to vertebrate TIA1 cytotoxic granule-associated RNA binding protein (TIA1) (zgc:55520) [Danio rerio] >gi_148726254, CAN88646.1, novel protein similar to vertebrate TIA1 cytotoxic granule-associated RNA binding protein (TIA1) (zgc:55520) [Danio rerio]

416 207663 2.5E-06 0.6 gi_41281615, NP_571395.1, plastin-2 [Danio rerio] >gi_73621406, Q6P698.1, PLSL_DANRE RecName: 97 < 1.0E-180 960 Full=Plastin-2; AltName: Full=L-plastin; AltName: Full=Lymphocyte cytosolic plastin 1 >gi_38566164, AAH62381.1, Lymphocyte cytosolic plastin 1 [Danio rerio]

417 207648 4.9E-02 0.6 gi_41281615, NP_571395.1, plastin-2 [Danio rerio] >gi_73621406, Q6P698.1, PLSL_DANRE RecName: 97 < 1.0E-180 958 Full=Plastin-2; AltName: Full=L-plastin; AltName: Full=Lymphocyte cytosolic plastin 1 >gi_38566164, AAH62381.1, Lymphocyte cytosolic plastin 1 [Danio rerio]

418 21229 1.4E-05 0.6 gi_410930448, XP_003978610.1, PREDICTED: uncharacterized protein LOC101076923 [Takifugu 55 1.99E-91 307 rubripes] 419 202596 8.6E-07 0.6 gi_410927452, XP_003977160.1, PREDICTED: protein THEMIS2-like [Takifugu rubripes] 71 1.15E-150 483 420 746684 6.7E-06 0.6 gi_410920539, XP_003973741.1, PREDICTED: ras association domain-containing protein 5-like 79 2.76E-114 371 [Takifugu rubripes] 421 23717 3.5E-03 0.6 gi_410904127, XP_003965544.1, PREDICTED: calcineurin B homologous protein 3-like [Takifugu 78 3.78E-93 303 rubripes] 422 531137 1.3E-04 0.6 gi_410898265, XP_003962618.1, PREDICTED: protein CLN8-like [Takifugu rubripes] 88 1.90E-145 453 423 351175 3.5E-02 0.6 gi_410897355, XP_003962164.1, PREDICTED: serum deprivation-response protein-like [Takifugu 74 6.56E-127 421 rubripes] 424 57574 2.0E-02 0.6 gi_4091078, AAC98961.1, cytochrome P450 [Dicentrarchus labrax] 85 < 1.0E-180 805 425 758889 1.0E-05 0.6 gi_403296778, XP_003939272.1, PREDICTED: integrin beta-7 [Saimiri boliviensis boliviensis] 58 3.50E-110 374 426 138294 5.0E-02 0.6 gi_401664016, BAM36389.1, bloodthirsty-2 [Oplegnathus fasciatus] 74 1.67E-171 513 427 58456 2.1E-02 0.6 gi_371781688, CCD11006.1, chemokine CXCF1a [Oncorhynchus mykiss] 94 1.63E-37 146 428 344947 1.7E-02 0.6 gi_357575246, AET85182.1, TC1-like transposase [Cyprinus carpio] 62 4.18E-80 243 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 429 299810 1.8E-03 0.6 gi_348546345, XP_003460639.1, PREDICTED: E3 ubiquitin-protein ligase TRIM21-like, partial 71 5.98E-51 193 [Oreochromis niloticus] 430 59493 1.6E-03 0.6 gi_348543283, XP_003459113.1, PREDICTED: la-related protein 7-like [Oreochromis niloticus] 70 1.62E-141 447 431 509279 4.1E-09 0.6 gi_348543109, XP_003459026.1, PREDICTED: hypothetical protein LOC100710899 [Oreochromis 82 < 1.0E-180 2881 niloticus] 432 40339 5.3E-03 0.6 gi_348535666, XP_003455320.1, PREDICTED: endoplasmic reticulum aminopeptidase 1 [Oreochromis 86 < 1.0E-180 1419 niloticus] 433 556563 4.4E-05 0.6 gi_348525218, XP_003450119.1, PREDICTED: RING finger protein 213-like [Oreochromis niloticus] 77 < 1.0E-180 4356 434 279632 3.9E-03 0.6 gi_348524845, XP_003449933.1, PREDICTED: epidermis-type lipoxygenase 3-like [Oreochromis 76 4.76E-135 440 niloticus] 435 6224 2.3E-04 0.6 gi_348523758, XP_003449390.1, PREDICTED: dnaJ homolog subfamily C member 3-like [Oreochromis 91 < 1.0E-180 759 niloticus] 436 704246 7.3E-03 0.6 gi_348523363, XP_003449193.1, PREDICTED: proto-oncogene serine/threonine-protein kinase pim-1- 97 1.43E-137 428 like [Oreochromis niloticus] 437 10188 3.3E-02 0.6 gi_348518369, XP_003446704.1, PREDICTED: LOW QUALITY PROTEIN: CCAAT/enhancer-binding 83 < 1.0E-180 1124 protein zeta-like [Oreochromis niloticus] 438 54367 1.0E-12 0.6 gi_348514405, XP_003444731.1, PREDICTED: dedicator of cytokinesis protein 2-like [Oreochromis 91 < 1.0E-180 3015 niloticus] 439 120849 2.6E-02 0.6 gi_348513575, XP_003444317.1, PREDICTED: tetratricopeptide repeat protein 39C-like [Oreochromis 95 < 1.0E-180 1044 niloticus] 440 586731 2.8E-03 0.6 gi_348512294, XP_003443678.1, PREDICTED: hypothetical protein LOC100700550 [Oreochromis 62 7.51E-18 97 niloticus] 441 215477 2.8E-02 0.6 gi_348511305, XP_003443185.1, PREDICTED: hypothetical protein LOC100701357 [Oreochromis 88 < 1.0E-180 636 niloticus] 442 553274 6.7E-06 0.6 gi_348509300, XP_003442188.1, PREDICTED: hypothetical protein LOC100698035 [Oreochromis 50 3.82E-158 521 niloticus] 443 629384 8.6E-03 0.6 gi_348506331, XP_003440713.1, PREDICTED: tribbles homolog 2-like [Oreochromis niloticus] 95 2.13E-143 454 444 381778 8.1E-04 0.6 gi_327282131, XP_003225797.1, PREDICTED: b-cell scaffold protein with ankyrin repeats-like [Anolis 56 6.55E-36 157 carolinensis] 445 70385 8.6E-08 0.6 gi_292668070, ADE40848.1, TNF receptor superfamily member 5A precursor [Oncorhynchus mykiss] 94 4.88E-154 441 446 6174 1.9E-02 0.6 gi_291190548, NP_001167382.1, ribosome biogenesis protein BOP1 [Salmo salar] >gi_223647870, 98 < 1.0E-180 1470 ACN10693.1, Ribosome biogenesis protein BOP1 [Salmo salar] 447 748078 2.8E-03 0.6 gi_291190268, NP_001167222.1, Cytohesin-2 [Salmo salar] >gi_223648744, ACN11130.1, Cytohesin-2 99 < 1.0E-180 794 [Salmo salar] 448 655752 6.1E-08 0.6 gi_225707240, ACO09466.1, FK506-binding protein 4 [Osmerus mordax] 93 < 1.0E-180 704 449 630455 1.2E-10 0.6 gi_223649266, ACN11391.1, Transposable element Tcb1 transposase [Salmo salar] 81 4.21E-12 74 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 450 77786 3.8E-09 0.6 gi_221124107, XP_002161911.1, PREDICTED: similar to predicted protein [Hydra magnipapillata] 51 3.85E-32 116 451 675170 4.4E-03 0.6 gi_218931178, NP_001135345.1, DNA-directed RNA polymerase II subunit RPB3 [Salmo salar] 98 2.87E-132 396 >gi_209734758, ACI68248.1, DNA-directed RNA polymerase II subunit RPB3 [Salmo salar] >gi_223647266, ACN10391.1, DNA-directed RNA polymerase II subunit RPB3 [Salmo salar] >gi_223673145, ACN12754.1, DNA-directed RNA polymerase II subunit RPB3 [Salmo salar]

452 178232 2.7E-02 0.6 gi_213627840, AAI71627.1, Cherp protein [Danio rerio] 77 < 1.0E-180 825 453 327897 1.4E-02 0.6 gi_213515022, NP_001133145.1, protein phosphatase 1, regulatory (inhibitor) subunit 1b-like [Salmo 98 2.30E-28 118 salar] >gi_197632135, ACH70791.1, protein phosphatase 1, regulatory (inhibitor) subunit 1b-like [Salmo salar] 454 39263 1.7E-02 0.6 gi_213514450, NP_001133536.1, Abl interactor 1 [Salmo salar] >gi_209154392, ACI33428.1, Abl 89 < 1.0E-180 588 interactor 1 [Salmo salar] 455 38726 9.4E-03 0.6 gi_213513686, NP_001134899.1, FAM60A [Salmo salar] >gi_209737008, ACI69373.1, FAM60A [Salmo 99 1.78E-89 296 salar] 456 123746 2.6E-02 0.6 gi_213513495, NP_001133676.1, P2X purinoceptor 4 [Salmo salar] >gi_209154318, ACI33391.1, P2X 91 < 1.0E-180 712 purinoceptor 4 [Salmo salar] >gi_209154902, ACI33683.1, P2X purinoceptor 4 [Salmo salar] 457 33310 1.0E-03 0.6 gi_213513110, NP_001133872.1, Interferon-induced protein 44 [Salmo salar] >gi_209155650, 98 < 1.0E-180 860 ACI34057.1, Interferon-induced protein 44 [Salmo salar] 458 477841 1.5E-10 0.6 gi_213512576, NP_001135199.1, Bactericidal permeability-increasing protein precursor [Salmo salar] 99 1.89E-126 399 >gi_209153900, ACI33182.1, Bactericidal permeability-increasing protein precursor [Salmo salar] 459 667431 7.1E-07 0.6 gi_213511658, NP_001134463.1, CD82 antigen [Salmo salar] >gi_209733530, ACI67634.1, CD82 93 3.18E-137 427 antigen [Salmo salar] 460 280268 2.8E-04 0.6 gi_213510882, NP_001133975.1, Keratin, type I cytoskeletal 18 [Salmo salar] >gi_209156024, 95 < 1.0E-180 724 ACI34244.1, Keratin, type I cytoskeletal 18 [Salmo salar] 461 584773 3.8E-02 0.6 gi_209962466, ACJ02102.1, SPATA5 [Salmo salar] 72 4.71E-26 78 462 742529 1.3E-02 0.6 gi_209156226, ACI34345.1, CTP synthase 1 [Salmo salar] 99 < 1.0E-180 1119 463 412367 4.7E-03 0.6 gi_209154664, ACI33564.1, SRC-like-adapter [Salmo salar] 97 2.94E-180 544 464 132957 2.0E-02 0.6 gi_209152323, ACI33107.1, 4-aminobutyrate aminotransferase, mitochondrial precursor [Salmo salar] 99 < 1.0E-180 951 465 192995 7.9E-04 0.6 gi_189534092, XP_001343150.2, PREDICTED: regulator of G-protein signaling 14-like [Danio rerio] 63 1.35E-125 414 466 54041 4.4E-03 0.6 gi_189531040, XP_001921927.1, PREDICTED: protein Daple [Danio rerio] 57 2.01E-86 322 467 345200 1.8E-03 0.6 gi_185135539, NP_001117963.1, calpain 2 catalytic subunit [Oncorhynchus mykiss] >gi_50812447, 93 < 1.0E-180 1260 AAT81417.1, calpain 2 catalytic subunit [Oncorhynchus mykiss] 468 345208 2.4E-04 0.6 gi_185135539, NP_001117963.1, calpain 2 catalytic subunit [Oncorhynchus mykiss] >gi_50812447, 93 < 1.0E-180 1255 AAT81417.1, calpain 2 catalytic subunit [Oncorhynchus mykiss] 469 472618 7.3E-03 0.6 gi_185134290, NP_001117918.1, glycogen synthase kinase binding protein [Oncorhynchus mykiss] 81 1.05E-75 254 >gi_37544637, AAM44075.1, guanylate-binding protein [Oncorhynchus mykiss] 470 530507 5.9E-03 0.6 gi_125837179, XP_697032.2, PREDICTED: hypothetical protein LOC568604 [Danio rerio] 62 4.26E-42 166 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 471 611566 1.5E-03 0.6 gi_125818692, XP_696949.2, PREDICTED: hypothetical protein LOC568525 [Danio rerio] 60 5.91E-27 117 472 551506 2.6E-02 0.5 gi_94733704, CAK11184.1, novel protein [Danio rerio] >gi_94734302, CAK11220.1, novel protein [Danio 67 < 1.0E-180 572 rerio] 473 62787 2.3E-03 0.5 gi_63101817, AAH95174.1, Zgc:136346 protein [Danio rerio] 76 2.41E-30 133 474 62803 7.4E-06 0.5 gi_63101817, AAH95174.1, Zgc:136346 protein [Danio rerio] 76 1.92E-30 133 475 8272 2.6E-07 0.5 gi_56090180, NP_001007773.1, actin-related protein 2 [Danio rerio] >gi_55715814, AAH85445.1, 96 1.96E-171 519 Zgc:101810 [Danio rerio] 476 274671 1.2E-05 0.5 gi_5579035, CAB51372.1, transposase [Pleuronectes platessa] 68 2.84E-10 72 477 257281 3.0E-15 0.5 gi_47086783, NP_997791.1, septin-6 [Danio rerio] >gi_34193920, AAH56592.1, Septin 6 [Danio rerio] 98 < 1.0E-180 716 478 72272 2.2E-05 0.5 gi_4502549, NP_001734.1, calmodulin [Homo sapiens] >gi_5901912, NP_008819.1, calmodulin [Homo 100 7.15E-93 297 sapiens] >gi_6680832, NP_031615.1, calmodulin [Mus musculus] >gi_6680834, NP_031616.1, calmodulin [Mus musculus] >gi_6753244, NP_033920.1, calmodulin [Mus musculus] >gi_6978591, NP_036650.1, calmodulin [Rattus norvegicus] >gi_8394168, NP_059022.1, calmodulin [Rattus norvegicus] >gi_14010863, NP_114175.1, calmodulin [Rattus norvegicus] >gi_33667057, NP_892012.1, calmodulin 3a [Danio rerio] >gi_41054633, NP_955864.1, calmodulin [Danio rerio] >gi_41152492, NP_956290.1, calmodulin 2a [Danio rerio] >gi_41152496, NP_956376.1, calmodulin 1b [Danio rerio] >gi_47087005, NP_998516.1, calmodulin 1a [Danio rerio] >gi_47550761, NP_999901.1, calmodulin 2, beta (phosphorylase kinase, delta) [Danio rerio] >gi_56118468, NP_001008160.1, calmodulin 2 (phosphorylase kinase, delta) [Xenopus (Silurana) tropicalis] >gi_57619286, NP_001009759.1, calmodulin [Ovis aries] >gi_58218968, NP_005175.2, calmodulin [Homo sapiens] >gi_114053089, NP_001039714.1, calmodulin [Bos taurus] >gi_148225823, NP_001080864.1, calmodulin [Xenopus laevis] >gi_148230184, NP_001089059.1, calmodulin 1 (phosphorylase kinase, delta) [Xenopus laevis] >gi_148236373, NP_001084025.1, calmodulin [Xenopus laevis] >gi_148727339, NP_001092028.1, calmodulin 2 (phosphorylase kinase, delta) [Pan troglodytes] >gi_197099032, NP_001125955.1, calmodulin [Pongo abelii] >gi_197100020, NP_001125755.1, calmodulin [Pongo abelii] >gi_197100741, NP_001126243.1, calmodulin [Pongo abelii] >gi_213512460, NP_001133185.1, calmodulin 2 (phosphorylase kinase, delta)-1 [Salmo salar] >gi_213512632, NP_001133186.1, calmodulin 2 (phosphorylase kinase, delta)-3 [Salmo salar] >gi_290542303, NP_001166505.1, calmodulin [Cavia porcellus] >gi_307548853, NP_001182569.1, calmodulin [Oryctolagus cuniculus] >gi_310750331, NP_001185528.1, calmodulin [Taeniopygia guttata] >gi_336020389, NP_001229501.1, calmodulin [Bos taurus] >gi_336088654, NP_001229516.1, calmodulin [Bos taurus] >gi_346644743, NP_001231138.1, calmodulin 3 [Sus scrofa] >gi_346644746, NP_001231139.1, calmodulin 1 [Sus scrofa] >gi_350538779, NP_001232586.1, putative calmodulin 1 variant 2 [Taeniopygia guttata] >gi_356991262, NP_001239360.1, calmodulin 2 [Cavia porcellus] >gi_57036407, XP_533635.1, PREDICTED: calmodulin isoform 1 [Canis lupus familiaris] >gi_57090327, XP_537537.1, PREDICTED: calmodulin isoform 1 [Canis lupus familiaris] >gi_109066549, XP_001109440.1, PREDICTED: calmodulin-like [Macaca mulatta] >gi_109102867, XP_001113516.1, PREDICTED: calmodulin-like isoform 5 [Macaca mulatta] >gi_109125252, XP_001112374.1, PREDICTED: calmodulin-2-like isoform 4 [Macaca mulatta] >gi_126303891, XP_001375537.1, PREDICTED: calmodulin-like [Monodelphis domestica] >gi_149408608, XP_001506524.1, PREDICTED: calmodulin-like isoform 1 [Ornithorhynchus anatinus] >gi_296215721, XP_002754240.1, PREDICTED: calmodulin-like [Callithrix jacchus] >gi_296223919, XP_002757824.1, PREDICTED: calmodulin-like isoform 1 [Callithrix jacchus] >gi_297265955, XP_001113408.2, PREDICTED: calmodulin-like isoform 1 [Macaca mulatta] >gi_297298442, XP_001087048.2, PREDICTED: calmodulin-like isoform 1 [Macaca mulatta] >gi_301605597, XP_002932424.1, PREDICTED: calmodulin-like isoform 1 [Xenopus (Silurana) tropicalis] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score >gi_301605599, XP_002932425.1, PREDICTED: calmodulin-like isoform 2 [Xenopus (Silurana) tropicalis] >gi_301753258, XP_002912465.1, PREDICTED: calmodulin-like isoform 1 [Ailuropoda melanoleuca] >gi_301753260, XP_002912466.1, PREDICTED: calmodulin-like isoform 2 [Ailuropoda melanoleuca] >gi_301770831, XP_002920827.1, PREDICTED: calmodulin-like [Ailuropoda melanoleuca] >gi_301775368, XP_002923096.1, PREDICTED: calmodulin-like isoform 2 [Ailuropoda melanoleuca] >gi_311252670, XP_003125211.1, PREDICTED: calmodulin-like [Sus scrofa] >gi_327265462, XP_003217527.1, PREDICTED: calmodulin-like [Anolis carolinensis] >gi_332223526, XP_003260924.1, PREDICTED: calmodulin-like [Nomascus leucogenys] >gi_332227376, XP_003262870.1, PREDICTED: calmodulin-like isoform 1 [Nomascus leucogenys] >gi_332227378, XP_003262871.1, PREDICTED: calmodulin-like isoform 2 [Nomascus leucogenys] >gi_332227380, XP_003262872.1, PREDICTED: calmodulin-like isoform 3 [Nomascus leucogenys] >gi_332227382, XP_003262873.1, PREDICTED: calmodulin-like isoform 4 [Nomascus leucogenys] >gi_332227384, XP_003262874.1, PREDICTED: calmodulin-like isoform 5 [Nomascus leucogenys] >gi_332227386, XP_003262875.1, PREDICTED: calmodulin-like isoform 6 [Nomascus leucogenys] >gi_332856296, XP_512771.3, PREDICTED: calmodulin isoform 3 [Pan troglodytes] >gi_332856304, XP_003316504.1, PREDICTED: calmodulin isoform 2 [Pan troglodytes] >gi_334310730, XP_001371508.2, PREDICTED: calmodulin-like [Monodelphis domestica] >gi_338710545, XP_001500896.3, PREDICTED: calmodulin- like [Equus caballus] >gi_338714301, XP_003363042.1, PREDICTED: calmodulin-like [Equus caballus] >gi_344274122, XP_003408867.1, PREDICTED: calmodulin-like [Loxodonta africana] >gi_344291760, XP_003417598.1, PREDICTED: calmodulin-like [Loxodonta africana] >gi_345304831, XP_003428265.1, PREDICTED: calmodulin-like isoform 2 [Ornithorhynchus anatinus] >gi_345777291, XP_864645.2, PREDICTED: calmodulin isoform 2 [Canis lupus familiaris] >gi_348506378, XP_003440736.1, PREDICTED: calmodulin-like [Oreochromis niloticus] >gi_348515921, XP_003445488.1, PREDICTED: calmodulin-like [Oreochromis niloticus] >gi_348526692, XP_003450853.1, PREDICTED: calmodulin-like [Oreochromis niloticus] >gi_348529738, XP_003452370.1, PREDICTED: calmodulin-like [Oreochromis niloticus] >gi_390474497, XP_003734788.1, PREDICTED: calmodulin-like isoform 2 [Callithrix jacchus] >gi_395503716, XP_003756209.1, PREDICTED: calmodulin-like [Sarcophilus harrisii] >gi_395827692, XP_003787031.1, PREDICTED: calmodulin-like isoform 1 [Otolemur garnettii] >gi_395827694, XP_003787032.1, PREDICTED: calmodulin-like isoform 2 [Otolemur garnettii] >gi_395829612, XP_003787943.1, PREDICTED: calmodulin-like isoform 1 [Otolemur garnettii] >gi_395829614, XP_003787944.1, PREDICTED: calmodulin-like isoform 2 [Otolemur garnettii] >gi_397493277, XP_003817536.1, PREDICTED: calmodulin-like isoform 1 [Pan paniscus] >gi_397493279, XP_003817537.1, PREDICTED: calmodulin-like isoform 2 [Pan paniscus] >gi_397504254, XP_003822716.1, PREDICTED: calmodulin-like isoform 1 [Pan paniscus] >gi_397504256, XP_003822717.1, PREDICTED: calmodulin-like isoform 2 [Pan paniscus] >gi_397504258, XP_003822718.1, PREDICTED: calmodulin-like isoform 3 [Pan paniscus] >gi_397504260, XP_003822719.1, PREDICTED: calmodulin-like isoform 4 [Pan paniscus] >gi_397504262, XP_003822720.1, PREDICTED: calmodulin-like isoform 5 [Pan paniscus] >gi_397504264, XP_003822721.1, PREDICTED: calmodulin-like isoform 6 [Pan paniscus] >gi_397525731, XP_003832809.1, PREDICTED: calmodulin-like isoform 1 [Pan paniscus] >gi_402876941, XP_003902206.1, PREDICTED: calmodulin [Papio anubis] >gi_402890789, XP_003908656.1, PREDICTED: calmodulin isoform 1 [Papio anubis] >gi_402890791, XP_003908657.1, PREDICTED: calmodulin isoform 2 [Papio anubis] >gi_402890793, XP_003908658.1, PREDICTED: calmodulin isoform 3 [Papio anubis] >gi_402890797, XP_003908660.1, PREDICTED: calmodulin isoform 5 [Papio anubis] >gi_402890799, XP_003908661.1, PREDICTED: calmodulin isoform 6 [Papio anubis] >gi_402890801, XP_003908662.1, PREDICTED: calmodulin isoform 7 [Papio anubis] >gi_402890803, XP_003908663.1, PREDICTED: calmodulin isoform 8 [Papio anubis] >gi_402890805, XP_003908664.1, PREDICTED: calmodulin isoform 9 [Papio anubis] >gi_402890807, XP_003908665.1, PREDICTED: calmodulin isoform 10 [Papio anubis] >gi_402906026, XP_003915808.1, PREDICTED: calmodulin-like [Papio anubis] >gi_403269554, XP_003926790.1, PREDICTED: calmodulin isoform 1 [Saimiri boliviensis AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score boliviensis] >gi_403269556, XP_003926791.1, PREDICTED: calmodulin isoform 2 [Saimiri boliviensis boliviensis] >gi_403299081, XP_003940320.1, PREDICTED: calmodulin [Saimiri boliviensis boliviensis] >gi_410900364, XP_003963666.1, PREDICTED: calmodulin-like [Takifugu rubripes] >gi_410909862, XP_003968409.1, PREDICTED: calmodulin-like [Takifugu rubripes] >gi_410916061, XP_003971505.1, PREDICTED: calmodulin-like [Takifugu rubripes] >gi_410954729, XP_003984014.1, PREDICTED: calmodulin [Felis catus] >gi_410982674, XP_003997674.1, PREDICTED: calmodulin [Felis catus] >gi_49037408, sp, P62161.2, CALM_RAT RecName: Full=Calmodulin; Short=CaM >gi_49037461, sp, P62144.2, CALM_ANAPL RecName: Full=Calmodulin; Short=CaM >gi_49037465, sp, P62149.2, CALM_CHICK RecName: Full=Calmodulin; Short=CaM >gi_49037467, sp, P62151.2, CALM_TORCA RecName: Full=Calmodulin; Short=CaM >gi_49037471, sp, P62155.2, CALM_XENLA RecName: Full=Calmodulin; Short=CaM >gi_49037472, sp, P62156.2, CALM_ONCSP RecName: Full=Calmodulin; Short=CaM >gi_49037473, sp, P62157.2, CALM_BOVIN RecName: Full=Calmodulin; Short=CaM >gi_49037474, sp, P62158.2, CALM_HUMAN RecName: Full=Calmodulin; Short=CaM >gi_49037475, sp, P62160.2, CALM_RABIT RecName: Full=Calmodulin; Short=CaM >gi_49037483, sp, P62204.2, CALM_MOUSE RecName: Full=Calmodulin; Short=CaM >gi_52782718, sp, Q6PI52.3, CALM_DANRE RecName: Full=Calmodulin; Short=CaM >gi_62286510, sp, Q5RAD2.3, CALM_PONAB RecName: Full=Calmodulin; Short=CaM >gi_75072157, sp, Q6YNX6.3, CALM_SHEEP RecName: Full=Calmodulin; Short=CaM >gi_78099191, sp, Q6IT78.3, CALM_CTEID RecName: Full=Calmodulin; Short=CaM >gi_78099192, sp, Q5EHV7.3, CALM_GECJA RecName: Full=Calmodulin; Short=CaM >gi_78099194, sp, Q71UH6.3, CALM_PERFV RecName: Full=Calmodulin; Short=CaM >gi_345576, pir, , JC1305 calmodulin - Japanese medaka >gi_15988184, pdb, 1IQ5, A Chain A, CalmodulinNEMATODE CA2+CALMODULIN DEPENDENT KINASE KINASE Fragment >gi_27065468, pdb, 1LVC, D Chain D, Crystal Structure Of The Adenylyl Cyclase Domain Of Anthrax Edema Factor (Ef) In Complex With Calmodulin And 2' Deoxy, 3' Anthraniloyl Atp >gi_27065469, pdb, 1LVC, E Chain E, Crystal Structure Of The Adenylyl Cyclase Domain Of Anthrax Edema Factor (Ef) In Complex With Calmodulin And 2' Deoxy, 3' Anthraniloyl Atp >gi_27065470, pdb, 1LVC, F Chain F, Crystal Structure Of The Adenylyl Cyclase Domain Of Anthrax Edema Factor (Ef) In Complex With Calmodulin And 2' Deoxy, 3' Anthraniloyl Atp >gi_71042646, pdb, 2BKI, B Chain B, Myosin Vi Nucleotide-Free (Mdinsert2-Iq) Crystal Structure >gi_71042647, pdb, 2BKI, D Chain D, Myosin Vi Nucleotide-Free (Mdinsert2-Iq) Crystal Structure >gi_93278425, pdb, 1WRZ, A Chain A, Calmodulin Complexed With A Peptide From A Human Death- Associated Protein Kinase >gi_157836465, pdb, 2V01, A Chain A, Recombinant Vertebrate Calmodulin Complexed With Pb >gi_157836466, pdb, 2V02, A Chain A, Recombinant Vertebrate Calmodulin Complexed With Ba >gi_193506549, pdb, 2R28, A Chain A, The Complex Structure Of Calmodulin Bound To A Calcineurin Peptide >gi_193506551, pdb, 2R28, B Chain B, The Complex Structure Of Calmodulin Bound To A Calcineurin Peptide >gi_237640385, pdb, 2W73, A Chain A, High-Resolution Structure Of The Complex Between Calmodulin And A Peptide From Calcineurin A >gi_237640386, pdb, 2W73, B Chain B, High-Resolution Structure Of The Complex Between Calmodulin And A Peptide From Calcineurin A >gi_237640387, pdb, 2W73, E Chain E, High-Resolution Structure Of The Complex Between Calmodulin And A Peptide From Calcineurin A >gi_237640388, pdb, 2W73, F Chain F, High- Resolution Structure Of The Complex Between Calmodulin And A Peptide From Calcineurin A >gi_257471927, pdb, 3HR4, B Chain B, Human Inos Reductase And Calmodulin Complex >gi_257471929, pdb, 3HR4, D Chain D, Human Inos Reductase And Calmodulin Complex >gi_257471931, pdb, 3HR4, F Chain F, Human Inos Reductase And Calmodulin Complex >gi_257471933, pdb, 3HR4, H Chain H, Human Inos Reductase And Calmodulin Complex >gi_310942919, pdb, 3OXQ, A Chain A, Crystal Structure Of Ca2+CAM-Cav1.2 Pre-IqIQ DOMAIN COMPLEX >gi_310942920, pdb, 3OXQ, B Chain B, Crystal Structure Of Ca2+CAM-Cav1.2 Pre-IqIQ DOMAIN COMPLEX >gi_310942921, pdb, 3OXQ, C Chain C, Crystal Structure Of Ca2+CAM-Cav1.2 Pre-IqIQ DOMAIN COMPLEX >gi_310942922, pdb, 3OXQ, D Chain D, Crystal Structure Of Ca2+CAM- Cav1.2 Pre-IqIQ DOMAIN COMPLEX >gi_365812921, pdb, 2Y4V, A Chain A, Crystal Structure Of Human Calmodulin In Complex With A Dap Kinase-1 Mutant (W305y) Peptide >gi_388603932, pdb, AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 3SJQ, A Chain A, Crystal Structure Of A Small Conductance Potassium Channel Splice Variant Complexed With Calcium-Calmodulin >gi_388603933, pdb, 3SJQ, B Chain B, Crystal Structure Of A Small Conductance Potassium Channel Splice Variant Complexed With Calcium-Calmodulin >gi_392935623, pdb, 4DCK, B Chain B, Crystal Structure Of The C-Terminus Of Voltage-Gated Sodium Channel In Complex With Fgf13 And Cam >gi_404312754, pdb, 3SUI, A Chain A, Crystal Structure Of Ca2+-Calmodulin In Complex With A Trpv1 C- Terminal Peptide >gi_404312965, pdb, 4G27, R Chain R, Calcium-Calmodulin Complexed With The Calmodulin Binding Domain From A Small Conductance Potassium Channel Splice Variant And Phenylurea >gi_404312967, pdb, 4G28, R Chain R, Calcium- Calmodulin Complexed With The Calmodulin Binding Domain From A Small Conductance Potassium Channel Splice Variant And Ebio-1 >gi_5542035, gb, AAD45181.1, AC006536_1 calmodulin [Homo sapiens] >gi_5901755, gb, AAD55398.1, AF178845_1 calmodulin [Rattus norvegicus] >gi_50274, emb, CAA43674.1, calmodulin [Mus musculus] >gi_55867, emb, CAA32478.1, calmodulin III [Rattus norvegicus] >gi_57041, emb, CAA32120.1, calmodulin [Rattus norvegicus] >gi_57835, emb, CAA32050.1, calmodulin [Rattus norvegicus] >gi_179810, gb, AAA35635.1, calmodulin [Homo sapiens] >gi_179884, gb, AAA35641.1, calmodulin [Homo sapiens] >gi_179888, gb, AAA51918.1, calmodulin [Homo sapiens] >gi_192365, gb, AAA37365.1, calmodulin synthesis [Mus musculus] >gi_203256, gb, AAA40862.1, calmodulin [Rattus norvegicus] >gi_203258, gb, AAA40863.1, calmodulin [Rattus norvegicus] >gi_203260, gb, AAA40864.1, calmodulin [Rattus norvegicus] >gi_207977, gb, AAA72214.1, calmodulin [synthetic construct] >gi_211386, gb, AAA48650.1, calmodulin [Gallus gallus] >gi_211398, gb, AAA48653.1, calmodulin [Gallus gallus] >gi_214017, gb, AAA49668.1, calmodulin (cDNA clone 11G2) [Xenopus laevis] >gi_214019, gb, AAA49669.1, calmodulin (cDNA clone 71) [Xenopus laevis] >gi_469420, gb, AAA66181.1, calmodulin [Mus musculus] >gi_531827, gb, AAB60644.1, calmodulin [Homo sapiens] >gi_665588, dbj, BAA08302.1, calmodulin [Homo sapiens] >gi_818020, emb, CAA32062.1, calmodulin II [Rattus norvegicus] >gi_1199919, dbj, BAA11896.1, calmodulin [Anas platyrhynchos] >gi_1334203, emb, CAA32119.1, calmodulin [Rattus norvegicus] >gi_2055425, gb, AAC83174.1, calmodulin [Homo sapiens] >gi_3719319, gb, AAC63306.1, calmodulin [Perca flavescens] >gi_12653369, gb, AAH00454.1, Calmodulin 1 (phosphorylase kinase, delta) [Homo sapiens] >gi_12836015, dbj, BAB23462.1, unnamed protein product [Mus musculus] >gi_12848868, dbj, BAB28116.1, unnamed protein product [Mus musculus] >gi_12849387, dbj, BAB28319.1, unnamed protein product [Mus musculus] >gi_12850203, dbj, BAB28631.1, unnamed protein product [Mus musculus] >gi_13097165, gb, AAH03354.1, Calmodulin 2 (phosphorylase kinase, delta) [Homo sapiens] >gi_13477325, gb, AAH05137.1, Calmodulin 3 (phosphorylase kinase, delta) [Homo sapiens] >gi_13623675, gb, AAH06464.1, Calmodulin 2 (phosphorylase kinase, delta) [Homo sapiens] >gi_14250335, gb, AAH08597.1, Calmodulin 1 (phosphorylase kinase, delta) [Homo sapiens] >gi_15080116, gb, AAH11834.1, Calmodulin 1 (phosphorylase kinase, delta) [Homo sapiens] >gi_15667249, gb, AAL02363.1, calmodulin 2 [Ovis aries] >gi_16924229, gb, AAH17385.1, Calmodulin 2 (phosphorylase kinase, delta) [Homo sapiens] >gi_17391486, gb, AAH18677.1, Calmodulin 2 (phosphorylase kinase, delta) [Homo sapiens] >gi_18204696, gb, AAH21347.1, Calmodulin 2 [Mus musculus] >gi_19913529, gb, AAH26065.1, Calmodulin 2 (phosphorylase kinase, delta) [Homo sapiens] >gi_26353076, dbj, BAC40168.1, unnamed protein product [Mus musculus] >gi_27881896, gb, AAH44434.1, Calmodulin 3b (phosphorylase kinase, delta) [Danio rerio] >gi_28189857, dbj, BAC56543.1, similar to calmodulin [Bos taurus] >gi_28278823, gb, AAH45298.1, Calmodulin 3a (phosphorylase kinase, delta) [Danio rerio] >gi_29748074, gb, AAH50926.1, Calmodulin 3 479 220485 2.9E-02 0.5 gi_410927482, XP_003977173.1, PREDICTED: probable serine/threonine-protein kinase kinX-like 70 3.03E-17 91 [Takifugu rubripes] 480 350493 5.1E-06 0.5 gi_410924910, XP_003975924.1, PREDICTED: caspase-8-like [Takifugu rubripes] 60 6.86E-63 221 481 259941 4.7E-02 0.5 gi_410922106, XP_003974524.1, PREDICTED: uncharacterized protein LOC101068942 [Takifugu 51 1.73E-13 80 rubripes] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 482 346489 8.3E-03 0.5 gi_410908335, XP_003967646.1, PREDICTED: tyrosine-protein phosphatase non-receptor type 9-like 93 4.77E-118 394 [Takifugu rubripes] 483 6053 1.5E-02 0.5 gi_410898571, XP_003962771.1, PREDICTED: grainyhead-like protein 3 homolog [Takifugu rubripes] 66 5.78E-82 273 484 395715 2.4E-09 0.5 gi_407027877, NP_001258388.1, myelin basic protein isoform 2 [Danio rerio] 70 6.79E-35 142 485 233126 5.5E-02 0.5 gi_363743834, XP_003642929.1, PREDICTED: homer protein homolog 3 isoform 1 [Gallus gallus] 92 1.04E-82 224 486 22147 5.4E-11 0.5 gi_350529345, NP_001070178.2, 12-lipoxygenase [Danio rerio] 76 < 1.0E-180 633 487 214625 1.4E-03 0.5 gi_348545298, XP_003460117.1, PREDICTED: uncharacterized protein K02A2.6-like [Oreochromis 74 < 1.0E-180 1122 niloticus] 488 155114 4.3E-02 0.5 gi_348545066, XP_003460001.1, PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A 93 < 1.0E-180 608 homolog [Oreochromis niloticus] 489 45154 6.6E-05 0.5 gi_348540684, XP_003457817.1, PREDICTED: proto-oncogene vav-like [Oreochromis niloticus] 89 < 1.0E-180 1303 490 87296 4.2E-02 0.5 gi_348537614, XP_003456288.1, PREDICTED: protein CIP2A homolog [Oreochromis niloticus] 83 < 1.0E-180 608 491 498928 5.3E-02 0.5 gi_348531623, XP_003453308.1, PREDICTED: heterogeneous nuclear ribonucleoprotein Q-like 98 < 1.0E-180 827 [Oreochromis niloticus] 492 391781 3.4E-02 0.5 gi_348530012, XP_003452505.1, PREDICTED: hepatoma-derived growth factor-like [Oreochromis 82 5.70E-92 307 niloticus] 493 370129 9.2E-08 0.5 gi_348529084, XP_003452044.1, PREDICTED: myoferlin-like [Oreochromis niloticus] 89 < 1.0E-180 1550 494 266440 1.6E-02 0.5 gi_348528346, XP_003451679.1, PREDICTED: proactivator polypeptide-like [Oreochromis niloticus] 57 1.86E-72 245 495 556594 7.0E-03 0.5 gi_348525218, XP_003450119.1, PREDICTED: RING finger protein 213-like [Oreochromis niloticus] 77 < 1.0E-180 4603 496 486931 2.4E-06 0.5 gi_348524434, XP_003449728.1, PREDICTED: hypothetical protein LOC100690846 [Oreochromis 55 1.87E-19 98 niloticus] 497 289458 < 1.0E-15 0.5 gi_348519379, XP_003447208.1, PREDICTED: double-strand-break repair protein rad21 homolog 88 < 1.0E-180 989 isoform 1 [Oreochromis niloticus] 498 365581 1.1E-07 0.5 gi_348517425, XP_003446234.1, PREDICTED: runt-related transcription factor 3-like [Oreochromis 88 < 1.0E-180 661 niloticus] 499 330656 6.1E-03 0.5 gi_348516399, XP_003445726.1, PREDICTED: serine/threonine-protein phosphatase 2A 55 kDa 97 < 1.0E-180 819 regulatory subunit B alpha isoform-like [Oreochromis niloticus] 500 330507 1.2E-03 0.5 gi_348513286, XP_003444173.1, PREDICTED: synaptotagmin-like protein 1-like [Oreochromis niloticus] 78 6.12E-149 470 501 20078 1.5E-02 0.5 gi_348505986, XP_003440541.1, PREDICTED: PHD and RING finger domain-containing protein 1-like 91 5.38E-115 406 [Oreochromis niloticus] 502 318942 3.1E-03 0.5 gi_339515652, NP_001129727.2, GTP cyclohydrolase 1 [Danio rerio] 88 5.15E-126 404 503 219998 2.8E-02 0.5 gi_317418937, CBN80975.1, O-methyltransferase family 3 [Dicentrarchus labrax] 93 < 1.0E-180 840 504 109836 1.4E-02 0.5 gi_317016311, ADM34974.2, Toll-like receptor 21 [Epinephelus coioides] >gi_340539235, AEK49148.1, 76 < 1.0E-180 1078 TLR21 [Epinephelus coioides] 505 704269 1.0E-02 0.5 gi_308322179, ADO28227.1, serine/threonine-protein kinase pim-3 [Ictalurus furcatus] 94 2.33E-52 191 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 506 537611 1.0E-02 0.5 gi_291190462, NP_001167374.1, EF-hand domain-containing protein D2 [Salmo salar] >gi_223649490, 100 2.40E-115 371 ACN11503.1, EF-hand domain-containing protein D2 [Salmo salar] 507 72275 8.0E-04 0.5 gi_261824806, pdb, 3EWT, AChain A, Crystal Structure Of Calmodulin Complexed With A Peptide 98 3.47E-93 298 >gi_261824808, pdb, 3EWV, A Chain A, Crystal Structure Of Calmodulin Complexed With A Peptide 508 75437 6.7E-06 0.5 gi_259155188, NP_001158836.1, Interleukin-17 receptor A precursor [Salmo salar] >gi_223647648, 92 < 1.0E-180 1500 ACN10582.1, Interleukin-17 receptor A precursor [Salmo salar] 509 258159 5.7E-03 0.5 gi_259089123, NP_001158598.1, activator of basal transcription 1 [Oncorhynchus mykiss] 94 7.36E-69 239 >gi_225705190, ACO08441.1, Activator of basal transcription 1 [Oncorhynchus mykiss] 510 625020 7.1E-03 0.5 gi_225717198, ACO14445.1, Hepatic lectin [Esox lucius] 62 3.87E-69 248 511 580758 8.8E-10 0.5 gi_225708226, ACO09959.1, Rho GDP-dissociation inhibitor 2 [Osmerus mordax] 97 6.33E-116 355 512 634213 5.2E-03 0.5 gi_225708078, ACO09885.1, LAG1 longevity assurance homolog 2 [Osmerus mordax] 86 1.94E-162 497 513 172835 3.0E-03 0.5 gi_224613404, ACN60281.1, Fructose-1,6-bisphosphatase 1 [Salmo salar] 99 < 1.0E-180 655 514 744119 4.5E-06 0.5 gi_223648810, ACN11163.1, Acidic leucine-rich nuclear phosphoprotein 32 family member E [Salmo 98 2.09E-95 310 salar] 515 226942 1.1E-05 0.5 gi_223647762, ACN10639.1, Tropomyosin alpha-3 chain [Salmo salar] 100 6.64E-21 97 516 714849 2.4E-08 0.5 gi_213515524, NP_001133945.1, receptor-interacting serine/threonine-protein kinase 4 [Salmo salar] 96 < 1.0E-180 892 >gi_209155918, ACI34191.1, Receptor-interacting serine/threonine-protein kinase 4 [Salmo salar] 517 324223 4.5E-06 0.5 gi_213514256, NP_001134840.1, Nucleoside diphosphate kinase 3 precursor [Salmo salar] 98 4.03E-106 344 >gi_209736472, ACI69105.1, Nucleoside diphosphate kinase 3 [Salmo salar] 518 234636 1.5E-02 0.5 gi_213512735, NP_001134241.1, replication factor C subunit 4 [Salmo salar] >gi_209731766, 99 4.99E-168 498 ACI66752.1, Replication factor C subunit 4 [Salmo salar] 519 477881 1.9E-04 0.5 gi_213512576, NP_001135199.1, Bactericidal permeability-increasing protein precursor [Salmo salar] 98 < 1.0E-180 594 >gi_209153900, ACI33182.1, Bactericidal permeability-increasing protein precursor [Salmo salar] 520 656017 9.4E-03 0.5 gi_213512204, NP_001133268.1, serine/threonine-protein kinase pim-1 [Salmo salar] >gi_209148370, 100 < 1.0E-180 574 ACI32934.1, Serine/threonine-protein kinase Pim-1 [Salmo salar] 521 321223 5.9E-05 0.5 gi_213512082, NP_001133698.1, syncoilin [Salmo salar] >gi_209154978, ACI33721.1, Asparaginyl- 96 < 1.0E-180 1027 tRNA synthetase, cytoplasmic [Salmo salar] 522 714314 6.0E-05 0.5 gi_213511184, NP_001134312.1, serine/threonine-protein kinase VRK1 [Salmo salar] >gi_209732302, 98 < 1.0E-180 694 ACI67020.1, Serine/threonine-protein kinase VRK1 [Salmo salar] 523 395270 8.1E-09 0.5 gi_213510910, NP_001134848.1, YHR209W [Salmo salar] >gi_209736528, ACI69133.1, YHR209W 98 < 1.0E-180 557 [Salmo salar] 524 522006 4.3E-04 0.5 gi_213510794, NP_001134601.1, Eukaryotic translation initiation factor 6 [Salmo salar] >gi_209732378, 95 2.12E-132 413 ACI67058.1, Eukaryotic translation initiation factor 6 [Salmo salar] >gi_209734590, ACI68164.1, Eukaryotic translation initiation factor 6 [Salmo salar]

525 705745 4.6E-02 0.5 gi_209737000, ACI69369.1, Histone RNA hairpin-binding protein [Salmo salar] 100 9.16E-150 355 526 414633 2.2E-06 0.5 gi_209731266, ACI66502.1, Cytochrome b5 [Salmo salar] 98 5.78E-48 179 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 527 744359 5.0E-02 0.5 gi_209731058, ACI66398.1, FAM49B [Salmo salar] 100 1.52E-74 252 528 576339 3.1E-02 0.5 gi_209155478, ACI33971.1, SUMO-conjugating enzyme UBC9 [Salmo salar] 100 8.78E-112 343 529 707857 3.4E-05 0.5 gi_209153948, ACI33206.1, Transcription factor MafB [Salmo salar] >gi_223648010, ACN10763.1, 97 < 1.0E-180 593 Transcription factor MafB [Salmo salar] 530 344207 1.9E-05 0.5 gi_185135493, NP_001117688.1, alpha-2,8-polysialyltransferase IV [Oncorhynchus mykiss] 98 < 1.0E-180 705 >gi_31559534, BAC77411.1, alpha-2,8-polysialyltransferase IV [Oncorhynchus mykiss] 531 602996 4.7E-04 0.5 gi_157886012, CAP09442.1, novel protein (zgc:136399) [Danio rerio] 85 < 1.0E-180 651 532 221691 7.4E-03 0.4 gi_6572473, AAF17287.1, U45968_1G-protein [Oncorhynchus mykiss] 100 1.56E-100 324 533 330473 3.0E-02 0.4 gi_62122793, NP_001014328.1, leukemia inhibitory factor receptor alpha a precursor [Danio rerio] 64 < 1.0E-180 839 >gi_61403566, AAH91862.1, Leukemia inhibitory factor receptor alpha [Danio rerio] >gi_182890026, AAI65177.1, Lifra protein [Danio rerio]

534 51150 5.4E-02 0.4 gi_47222373, CAG05122.1, unnamed protein product [Tetraodon nigroviridis] 62 2.21E-113 377 535 752928 4.8E-03 0.4 gi_410929832, XP_003978303.1, PREDICTED: arrestin red cell isoform 1-like [Takifugu rubripes] 97 1.40E-142 461 536 202613 3.1E-05 0.4 gi_410927452, XP_003977160.1, PREDICTED: protein THEMIS2-like [Takifugu rubripes] 70 2.82E-151 484 537 743577 6.6E-03 0.4 gi_410913241, XP_003970097.1, PREDICTED: transcriptional repressor CTCF-like [Takifugu rubripes] 89 < 1.0E-180 886 538 196811 1.1E-02 0.4 gi_41053559, NP_956588.1, UDP-N-acetylhexosamine pyrophosphorylase-like protein 1 [Danio rerio] 85 < 1.0E-180 750 >gi_82209691, Q7ZWD4.1, UAP1L_DANRE RecName: Full=UDP-N-acetylhexosamine pyrophosphorylase-like protein 1 >gi_29436948, AAH49467.1, UDP-N-acteylglucosamine pyrophosphorylase 1, like 1 [Danio rerio] 539 511814 3.6E-02 0.4 gi_374110743, A7MBL8.1, PKN2_DANRERecName: Full=Serine/threonine-protein kinase N2; AltName: 86 < 1.0E-180 1448 Full=PKN gamma; AltName: Full=Protein kinase C-like 2; AltName: Full=Protein-kinase C-related kinase 2 >gi_156229814, AAI51830.1, Zgc:153916 protein [Danio rerio]

540 341278 9.7E-06 0.4 gi_350537161, NP_001233265.1, IL-4 receptor-2 precursor [Oncorhynchus mykiss] >gi_317453177, 95 < 1.0E-180 1163 CBL94861.1, IL-4 receptor-2 precursor [Oncorhynchus mykiss] 541 663013 6.1E-04 0.4 gi_348566387, XP_003468983.1, PREDICTED: calcineurin subunit B type 1-like [Cavia porcellus] 93 2.81E-94 310 542 264261 2.0E-12 0.4 gi_348542726, XP_003458835.1, PREDICTED: probable ATP-dependent RNA helicase DDX6-like 93 < 1.0E-180 832 [Oreochromis niloticus] 543 282847 5.1E-02 0.4 gi_348540030, XP_003457491.1, PREDICTED: serine/threonine-protein kinase OSR1-like [Oreochromis 90 < 1.0E-180 872 niloticus] 544 389478 1.0E-02 0.4 gi_348538756, XP_003456856.1, PREDICTED: NACHT, LRR and PYD domains-containing protein 3- 65 < 1.0E-180 639 like [Oreochromis niloticus] 545 687365 4.7E-02 0.4 gi_348537110, XP_003456038.1, PREDICTED: trifunctional purine biosynthetic protein adenosine-3-like 92 < 1.0E-180 1620 [Oreochromis niloticus] 546 8108 1.3E-06 0.4 gi_348535800, XP_003455386.1, PREDICTED: NAD kinase-like [Oreochromis niloticus] 92 < 1.0E-180 707 547 27026 1.1E-02 0.4 gi_348535431, XP_003455204.1, PREDICTED: peptidylprolyl isomerase domain and WD repeat- 96 < 1.0E-180 1120 containing protein 1 [Oreochromis niloticus] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 548 91118 1.4E-04 0.4 gi_348531635, XP_003453314.1, PREDICTED: membrane-bound O-acyltransferase domain-containing 87 < 1.0E-180 543 protein 2-like [Oreochromis niloticus] 549 102309 2.2E-02 0.4 gi_348529039, XP_003452022.1, PREDICTED: hypothetical protein LOC100706146 [Oreochromis 77 8.40E-161 493 niloticus] 550 116729 3.3E-02 0.4 gi_348528714, XP_003451861.1, PREDICTED: scavenger mRNA-decapping enzyme DcpS-like 86 3.54E-156 480 [Oreochromis niloticus] 551 493472 4.6E-02 0.4 gi_348518149, XP_003446594.1, PREDICTED: abl interactor 2-like isoform 3 [Oreochromis niloticus] 87 < 1.0E-180 597 552 84485 4.2E-04 0.4 gi_348516136, XP_003445595.1, PREDICTED: protein FAM101A-like [Oreochromis niloticus] 95 6.46E-100 325 553 313168 2.1E-05 0.4 gi_348515295, XP_003445175.1, PREDICTED: myosin-If [Oreochromis niloticus] 90 < 1.0E-180 1856 554 3175 9.1E-03 0.4 gi_348514399, XP_003444728.1, PREDICTED: hypothetical protein LOC100711292 [Oreochromis 89 < 1.0E-180 625 niloticus] 555 506869 4.4E-02 0.4 gi_348511307, XP_003443186.1, PREDICTED: hypothetical protein LOC100701629 [Oreochromis 91 < 1.0E-180 628 niloticus] 556 762330 3.7E-04 0.4 gi_348508762, XP_003441922.1, PREDICTED: eukaryotic elongation factor 2 kinase [Oreochromis 80 < 1.0E-180 889 niloticus] 557 541308 4.6E-02 0.4 gi_348507044, XP_003441067.1, PREDICTED: sorting nexin-5-like [Oreochromis niloticus] 96 < 1.0E-180 716 558 23561 1.4E-03 0.4 gi_348504056, XP_003439578.1, PREDICTED: 1-phosphatidylinositol-4,5-bisphosphate 90 < 1.0E-180 1491 phosphodiesterase gamma-2 [Oreochromis niloticus] 559 109246 3.4E-02 0.4 gi_348503035, XP_003439072.1, PREDICTED: uridine-cytidine kinase-like 1-like [Oreochromis niloticus] 97 < 1.0E-180 1001 560 591061 3.4E-06 0.4 gi_345111056, pdb, 3TGD, AChain A, Crystal Structure Of The Human Ubiquitin-Conjugating Enzyme 97 5.09E-64 220 (E2) Ubch5b 561 356951 5.4E-03 0.4 gi_326677540, XP_003200859.1, PREDICTED: semaphorin-4D-like [Danio rerio] 78 < 1.0E-180 1081 562 302203 6.7E-14 0.4 gi_326670904, XP_001920559.3, PREDICTED: poly [ADP-ribose] polymerase 15 [Danio rerio] 65 < 1.0E-180 812 563 751786 1.9E-04 0.4 gi_326669740, XP_003199072.1, PREDICTED: hypothetical protein LOC100538137 [Danio rerio] 63 < 1.0E-180 656 564 287374 2.8E-04 0.4 gi_301611322, XP_002935199.1, PREDICTED: hypothetical protein LOC100490020 [Xenopus (Silurana) 69 1.20E-43 174 tropicalis] 565 3853 4.0E-03 0.4 gi_292624080, XP_002665524.1, PREDICTED: carcinoembryonic antigen-related cell adhesion molecule 65 1.03E-140 457 5 [Danio rerio] 566 6036 6.9E-04 0.4 gi_292623243, XP_001332938.3, PREDICTED: grainyhead-like 3 [Danio rerio] 94 2.79E-09 69 567 403566 3.0E-15 0.4 gi_292617625, XP_686009.4, PREDICTED: leucine-rich repeat flightless-interacting protein 2 [Danio 85 2.56E-84 299 rerio] 568 606803 7.1E-09 0.4 gi_291190072, NP_001167174.1, helicase MOV-10 [Salmo salar] >gi_223648458, ACN10987.1, 98 < 1.0E-180 925 helicase MOV-10 [Salmo salar] 569 219528 1.9E-04 0.4 gi_284520970, NP_001165278.1, macrophage colony stimulating factor receptor-like protein precursor 96 < 1.0E-180 1823 [Salmo salar] >gi_256032184, ACU57063.1, macrophage colony stimulating factor receptor-like protein [Salmo salar] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 570 388581 8.7E-03 0.4 gi_260182175, ACX35603.1, hypothetical protein [Salmo salar] >gi_260182176, ACX35604.1, 59 4.17E-51 190 hypothetical protein [Salmo salar] >gi_260182181, ACX35609.1, hypothetical protein [Salmo salar] 571 394823 1.2E-03 0.4 gi_259155381, NP_001158752.1, Claudin-like protein ZF4A22 [Salmo salar] >gi_169640212, 96 9.84E-94 312 DAA06152.1, TPA_exp: claudin 7 [Salmo salar] >gi_223646908, ACN10212.1, Claudin-like protein ZF4A22 [Salmo salar] >gi_223672771, ACN12567.1, Claudin-like protein ZF4A22 [Salmo salar] >gi_339639545, DAA34901.1, TPA_exp: claudin 7 [Oncorhynchus mykiss]

572 376600 6.5E-05 0.4 gi_254911056, NP_001157171.1, retinoic acid-inducible gene-I [Salmo salar] >gi_254692471, 97 < 1.0E-180 1344 CAX48607.2, retinoic acid-inducible gene-I [Salmo salar] 573 569867 4.0E-05 0.4 gi_239985508, NP_001135285.1, Vacuolar proton translocating ATPase 116 kDa subunit a [Salmo salar] 95 < 1.0E-180 1180 >gi_209156028, ACI34246.1, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3 [Salmo salar] 574 271358 2.2E-06 0.4 gi_225708232, ACO09962.1, Cell division protein kinase 4 [Osmerus mordax] 94 4.65E-164 507 575 220804 8.7E-05 0.4 gi_224613288, ACN60223.1, Gelsolin precursor [Salmo salar] 97 < 1.0E-180 1019 576 481715 1.7E-06 0.4 gi_223647694, ACN10605.1, Twinfilin-1 [Salmo salar] 98 < 1.0E-180 611 577 297607 5.9E-04 0.4 gi_223647600, ACN10558.1, Actin-related protein 2-A [Salmo salar] >gi_223647732, ACN10624.1, 100 < 1.0E-180 758 Actin-related protein 2-A [Salmo salar] 578 200008 < 1.0E-15 0.4 gi_221221794, ACM09558.1, Proteasome activator complex subunit 1 [Salmo salar] 99 3.18E-115 352 579 741979 8.7E-09 0.4 gi_213514592, NP_001133511.1, FYN-binding protein [Salmo salar] >gi_209154290, ACI33377.1, FYN- 78 < 1.0E-180 705 binding protein [Salmo salar] 580 605956 1.5E-03 0.4 gi_213513479, NP_001133962.1, Myristoylated alanine-rich C-kinase substrate [Salmo salar] 95 1.12E-56 205 >gi_209155976, ACI34220.1, Myristoylated alanine-rich C-kinase substrate [Salmo salar] 581 85469 9.4E-03 0.4 gi_213513268, NP_001133617.1, Ras-related protein Rab-5A [Salmo salar] >gi_197631797, 98 2.31E-138 419 ACH70622.1, member RAS oncogene family [Salmo salar] >gi_209154698, ACI33581.1, Ras-related protein Rab-5A [Salmo salar]

582 581911 7.7E-03 0.4 gi_213512985, NP_001135259.1, Lamina-associated polypeptide 2 isoform beta [Salmo salar] 91 < 1.0E-180 584 >gi_209155494, ACI33979.1, Lamina-associated polypeptide 2 isoform beta [Salmo salar] 583 144693 8.2E-03 0.4 gi_213512839, NP_001133332.1, Keratinocytes-associated transmembrane protein 2 [Salmo salar] 92 1.67E-93 303 >gi_209150797, ACI33044.1, Keratinocytes-associated transmembrane protein 2 precursor [Salmo salar] 584 188790 3.8E-02 0.4 gi_213512578, NP_001133445.1, Vacuolar protein sorting-associated protein 26B-B [Salmo salar] 99 < 1.0E-180 659 >gi_209154030, ACI33247.1, Vacuolar protein sorting-associated protein 26B-B [Salmo salar] >gi_223649466, ACN11491.1, Vacuolar protein sorting-associated protein 26B-B [Salmo salar]

585 321234 9.7E-04 0.4 gi_213512082, NP_001133698.1, syncoilin [Salmo salar] >gi_209154978, ACI33721.1, Asparaginyl- 96 < 1.0E-180 649 tRNA synthetase, cytoplasmic [Salmo salar] 586 54111 3.5E-03 0.4 gi_211971033, NP_001130017.1, myeloid differentiation primary response protein MyD88 [Salmo salar] 97 < 1.0E-180 565 >gi_292630829, B3SRQ2.1, MYD88_SALSA RecName: Full=Myeloid differentiation primary response protein MyD88 >gi_157644541, ABV59003.1, MyD88 [Salmo salar]

587 113386 1.3E-03 0.4 gi_209731510, ACI66624.1, Anterior gradient protein 3 homolog precursor [Salmo salar] 100 2.54E-35 133 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 588 146880 4.4E-02 0.4 gi_209155870, ACI34167.1, Proactivator polypeptide precursor [Salmo salar] 94 5.99E-159 494 589 2764 5.8E-09 0.4 gi_209154804, ACI33634.1, Integral membrane protein 2C [Salmo salar] 100 1.32E-121 377 590 322817 3.2E-02 0.4 gi_209154488, ACI33476.1, Rho GDP-dissociation inhibitor 1 [Salmo salar] 86 2.44E-47 151 591 58364 4.6E-03 0.4 gi_185135737, NP_001117083.1, CD4-like protein isoform 1 precursor [Salmo salar] >gi_167538886, 93 < 1.0E-180 824 ABZ81916.1, CD4-1-like protein [Salmo salar] >gi_182410021, ACB88037.1, CD4-1-like protein [Salmo salar] 592 289870 1.2E-02 0.4 gi_16357472, NP_426359.1, cell division control protein 42 homolog isoform 2 [Homo sapiens] 76 7.24E-84 273 >gi_56118450, NP_001008027.1, cell division cycle 42 (GTP binding protein, 25kDa) [Xenopus (Silurana) tropicalis] >gi_344313177, NP_001230698.1, cell division control protein 42 homolog isoform 2 [Mus musculus] >gi_114554564, XP_001164385.1, PREDICTED: cell division control protein 42 homolog isoform 4 [Pan troglodytes] >gi_126328461, XP_001366319.1, PREDICTED: cell division control protein 42 homolog isoform 2 [Monodelphis domestica] >gi_291399320, XP_002716038.1, PREDICTED: cell division cycle 42 isoform 2 [Oryctolagus cuniculus] >gi_296206976, XP_002750450.1, PREDICTED: cell division control protein 42 homolog isoform 3 [Callithrix jacchus] >gi_326932586, XP_003212396.1, PREDICTED: cell division control protein 42 homolog [Meleagris gallopavo] >gi_332244946, XP_003271625.1, PREDICTED: cell division control protein 42 homolog isoform 2 [Nomascus leucogenys] >gi_338722129, XP_003364489.1, PREDICTED: cell division control protein 42 homolog [Equus caballus] >gi_354482996, XP_003503681.1, PREDICTED: cell division control protein 42 homolog isoform 2 [Cricetulus griseus] >gi_390465436, XP_003733407.1, PREDICTED: cell division control protein 42 homolog [Callithrix jacchus] >gi_395521643, XP_003764926.1, PREDICTED: cell division control protein 42 homolog isoform 1 [Sarcophilus harrisii] >gi_395821055, XP_003783864.1, PREDICTED: cell division control protein 42 homolog isoform 1 [Otolemur garnettii] >gi_395821057, XP_003783865.1, PREDICTED: cell division control protein 42 homolog isoform 2 [Otolemur garnettii] >gi_397485760, XP_003814008.1, PREDICTED: cell division control protein 42 homolog isoform 4 [Pan paniscus] >gi_397485766, XP_003814011.1, PREDICTED: cell division control protein 42 homolog isoform 7 [Pan paniscus] >gi_410966328, XP_003989685.1, PREDICTED: cell division control protein 42 homolog isoform 2 [Felis catus] >gi_6531681, AAF15538.1, AF205635_1 cell division cycle 42 [Rattus norvegicus] >gi_20379098, AAM21109.1, AF498962_1 small GTP binding protein CDC42 [Homo sapiens] >gi_182857, AAA52494.1, GTP-binding protein G25K [Homo sapiens] >gi_1321599, AAB40051.1, cdc42b [Mus musculus] >gi_6012989, CAB57325.1, hypothetical protein [Homo sapiens] >gi_31652314, CAD92551.1, cell division cycle 42 (GTP binding protein, 25kDa) [Homo sapiens] >gi_51703836, AAH80906.1, cell division cycle 42 (GTP binding protein, 25kDa) [Xenopus (Silurana) tropicalis] >gi_119615412, EAW95006.1, cell division cycle 42 (GTP binding protein, 25kDa), isoform CRA_c [Homo sapiens] >gi_119615414, EAW95008.1, cell division cycle 42 (GTP binding protein, 25kDa), isoform CRA_c [Homo sapiens] >gi_119615415, EAW95009.1, cell division cycle 42 (GTP binding protein, 25kDa), isoform CRA_c [Homo sapiens] >gi_123252260, CAM18514.1, cell division cycle 42 homolog (S. cerevisiae) [Mus musculus] >gi_149024338, EDL80835.1, cell division cycle 42 homolog (S. cerevisiae), isoform CRA_b [Rattus norvegicus] >gi_261861424, BAI47234.1, cell division cycle protein 42 [synthetic construct] >gi_351705995, EHB08914.1, Cell division control protein 42-like protein [Heterocephalus glaber] >gi_355557645, EHH14425.1, hypothetical protein EGK_00347 [Macaca mulatta] >gi_355745004, EHH49629.1, hypothetical protein EGM_00319 [Macaca fascicularis] >gi_380818556, AFE81151.1, cell division control protein 42 homolog isoform 2 [Macaca mulatta] 593 357033 1.1E-02 0.4 gi_125852188, XP_001343517.1, PREDICTED: sentrin-specific protease 1 [Danio rerio] 90 < 1.0E-180 415 594 390729 2.4E-02 0.3 gi_410921332, XP_003974137.1, PREDICTED: semaphorin-4E-like [Takifugu rubripes] 72 < 1.0E-180 885 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 595 26547 1.8E-02 0.3 gi_410912742, XP_003969848.1, PREDICTED: secretory carrier-associated membrane protein 2-like 85 2.57E-127 407 [Takifugu rubripes] 596 708005 1.4E-03 0.3 gi_410904477, XP_003965718.1, PREDICTED: uncharacterized protein LOC101071728 [Takifugu 70 2.70E-167 516 rubripes] 597 264902 4.8E-04 0.3 gi_410899068, XP_003963019.1, PREDICTED: mitogen-activated protein kinase 13-like [Takifugu 92 < 1.0E-180 651 rubripes] 598 377291 7.7E-03 0.3 gi_348544351, XP_003459645.1, PREDICTED: calcium/calmodulin-dependent protein kinase type II 97 < 1.0E-180 617 delta 1 chain-like isoform 2 [Oreochromis niloticus] 599 203680 1.9E-03 0.3 gi_348528340, XP_003451676.1, PREDICTED: mps one binder kinase activator-like 1B-like 98 8.07E-138 435 [Oreochromis niloticus] 600 709525 2.4E-02 0.3 gi_348520173, XP_003447603.1, PREDICTED: ADP-dependent glucokinase-like [Oreochromis niloticus] 93 < 1.0E-180 696 601 676489 2.0E-03 0.3 gi_348517829, XP_003446435.1, PREDICTED: retinoid-inducible serine carboxypeptidase-like 88 < 1.0E-180 677 [Oreochromis niloticus] 602 720122 5.2E-02 0.3 gi_348508416, XP_003441750.1, PREDICTED: beta-secretase 2-like [Oreochromis niloticus] 91 1.03E-154 491 603 678178 4.5E-02 0.3 gi_348507186, XP_003441137.1, PREDICTED: bifunctional protein NCOAT-like [Oreochromis niloticus] 88 < 1.0E-180 1470 604 310447 1.8E-02 0.3 gi_348506569, XP_003440831.1, PREDICTED: serine/threonine-protein phosphatase 2A 56 kDa 96 < 1.0E-180 955 regulatory subunit delta isoform-like [Oreochromis niloticus] 605 50001 2.4E-03 0.3 gi_348503191, XP_003439149.1, PREDICTED: tyrosine-protein phosphatase non-receptor type 1-like 78 < 1.0E-180 598 [Oreochromis niloticus] 606 393923 3.5E-03 0.3 gi_348502788, XP_003438949.1, PREDICTED: diacylglycerol kinase alpha-like [Oreochromis niloticus] 91 < 1.0E-180 872 607 690485 1.1E-02 0.3 gi_326670973, XP_002663332.2, PREDICTED: hypothetical protein LOC100330630 [Danio rerio] 47 4.75E-117 424 608 205405 3.1E-04 0.3 gi_259155162, NP_001158823.1, Serine/threonine-protein phosphatase 4 regulatory subunit 2-A [Salmo 98 3.63E-90 310 salar] >gi_223647572, ACN10544.1, Serine/threonine-protein phosphatase 4 regulatory subunit 2-A [Salmo salar] 609 296496 3.6E-02 0.3 gi_223647600, ACN10558.1, Actin-related protein 2-A [Salmo salar] >gi_223647732, ACN10624.1, 100 < 1.0E-180 758 Actin-related protein 2-A [Salmo salar] 610 308269 1.8E-03 0.3 gi_213514834, NP_001134536.1, Argininosuccinate synthase [Salmo salar] >gi_209734100, 98 < 1.0E-180 555 ACI67919.1, Argininosuccinate synthase [Salmo salar] 611 218219 1.5E-02 0.3 gi_213514660, NP_001133969.1, L-lactate dehydrogenase B chain [Salmo salar] >gi_209156006, 100 2.27E-141 442 ACI34235.1, L-lactate dehydrogenase B chain [Salmo salar] 612 590144 1.3E-07 0.3 gi_213513374, NP_001133504.1, polypyrimidine tract-binding protein 2 [Salmo salar] >gi_209154262, 99 3.11E-177 553 ACI33363.1, Polypyrimidine tract-binding protein 2 [Salmo salar] 613 309814 3.8E-03 0.3 gi_213512119, NP_001133668.1, Transmembrane protein 66 precursor [Salmo salar] >gi_209154874, 93 1.93E-111 353 ACI33669.1, Transmembrane protein 66 precursor [Salmo salar] 614 219241 2.0E-02 0.3 gi_213511989, NP_001133981.1, Soluble calcium-activated nucleotidase 1 [Salmo salar] 99 4.14E-120 392 >gi_209156050, ACI34257.1, Soluble calcium-activated nucleotidase 1 [Salmo salar] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 615 481675 1.7E-02 0.3 gi_213510974, NP_001133604.1, Twinfilin-1 [Salmo salar] >gi_209154652, ACI33558.1, Twinfilin-1 98 8.27E-115 356 [Salmo salar] 616 485758 2.0E-03 0.3 gi_209151380, ACI33073.1, X-box-binding protein 1 [Salmo salar] 96 5.96E-76 257 617 364945 1.1E-04 0.3 gi_185135222, NP_001117195.1, FXYD domain containing ion transport regulator 5a precursor [Salmo 62 3.42E-77 270 salar] >gi_167651261, DAA06141.1, TPA_exp: FXYD5a [Salmo salar] 618 679705 < 1.0E-15 0.3 gi_185132161, NP_001117703.1, heat shock 90kDa protein 1 beta isoform a [Oncorhynchus mykiss] 100 < 1.0E-180 889 >gi_60223015, BAD90023.1, heat shock 90kDa protein 1 beta isoform a [Oncorhynchus mykiss] 619 176082 4.9E-05 0.2 gi_410904525, XP_003965742.1, PREDICTED: septin-7-like [Takifugu rubripes] 93 < 1.0E-180 672 620 378454 1.3E-02 0.2 gi_348533149, XP_003454068.1, PREDICTED: hypothetical protein LOC100706773 [Oreochromis 63 1.38E-78 275 niloticus] 621 221678 2.5E-02 0.2 gi_344241758, EGV97861.1, GTPase KRas [Cricetulus griseus] 99 3.71E-83 282 622 296550 3.5E-03 0.2 gi_259089187, NP_001158631.1, Syntaxin-7 [Oncorhynchus mykiss] >gi_225705430, ACO08561.1, 98 1.57E-94 314 Syntaxin-7 [Oncorhynchus mykiss] 623 509629 7.6E-03 0.2 gi_213512855, NP_001133297.1, Cation-dependent mannose-6-phosphate receptor precursor [Salmo 94 9.77E-153 469 salar] >gi_209149767, ACI32990.1, Cation-dependent mannose-6-phosphate receptor precursor [Salmo salar] 624 330292 3.0E-03 -0.2 gi_210147448, NP_001002728.2, chaperone activity of bc1 complex-like, mitochondrial [Danio rerio] 87 < 1.0E-180 911 625 9733 3.8E-02 -0.3 gi_63101109, AAH95813.1, Zgc:136359 protein [Danio rerio] 85 < 1.0E-180 676 626 206813 1.1E-03 -0.3 gi_348526398, XP_003450706.1, PREDICTED: hypothetical protein LOC100711315 [Oreochromis 56 5.54E-89 296 niloticus] 627 114411 2.2E-03 -0.3 gi_348525030, XP_003450025.1, PREDICTED: tubulin beta chain-like [Oreochromis niloticus] 100 < 1.0E-180 788 628 68235 6.7E-04 -0.3 gi_348524486, XP_003449754.1, PREDICTED: ubiquitin carboxyl-terminal hydrolase 38 [Oreochromis 92 < 1.0E-180 887 niloticus] 629 493563 7.8E-04 -0.3 gi_348518149, XP_003446594.1, PREDICTED: abl interactor 2-like isoform 3 [Oreochromis niloticus] 87 < 1.0E-180 597 630 27583 2.2E-02 -0.3 gi_348509789, XP_003442429.1, PREDICTED: cGMP-inhibited 3',5'-cyclic phosphodiesterase B 85 < 1.0E-180 1339 [Oreochromis niloticus] 631 375194 1.4E-02 -0.3 gi_348505436, XP_003440267.1, PREDICTED: endoplasmic reticulum mannosyl-oligosaccharide 1,2- 83 < 1.0E-180 799 alpha-mannosidase-like [Oreochromis niloticus] 632 232010 5.4E-03 -0.3 gi_326674029, XP_001333228.3, PREDICTED: endothelin-converting enzyme 2 [Danio rerio] 96 < 1.0E-180 1283 633 742252 2.6E-02 -0.3 gi_225715518, ACO13605.1, Four and a half LIM domains protein 1 [Esox lucius] 97 < 1.0E-180 585 634 505439 1.0E-02 -0.3 gi_221219528, ACM08425.1, C-X-C motif chemokine 14 precursor [Salmo salar] 98 2.13E-47 175 635 207261 5.4E-03 -0.3 gi_213513475, NP_001133903.1, thioredoxin interacting protein [Salmo salar] >gi_197632235, 98 5.97E-124 400 ACH70841.1, thioredoxin interacting protein [Salmo salar] >gi_209155762, ACI34113.1, Thioredoxin- interacting protein [Salmo salar]

636 337059 7.8E-05 -0.3 gi_213512979, NP_001133388.1, Filamin-binding LIM protein 1 [Salmo salar] >gi_209152997, 100 8.01E-136 427 ACI33139.1, Filamin-binding LIM protein 1 [Salmo salar] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 637 527077 6.7E-03 -0.3 gi_213512582, NP_001133244.1, EH domain-containing protein 4 [Salmo salar] >gi_209147473, 98 < 1.0E-180 706 ACI32891.1, EH domain-containing protein 4 [Salmo salar] 638 295785 1.3E-10 -0.3 gi_213511987, NP_001134721.1, WW domain binding protein 2 [Salmo salar] >gi_209735452, 99 3.49E-91 300 ACI68595.1, WW domain-binding protein 2 [Salmo salar] 639 412278 8.7E-05 -0.3 gi_213511226, NP_001133826.1, Lysosomal-associated transmembrane protein 4A [Salmo salar] 93 8.81E-70 245 >gi_209155470, ACI33967.1, Lysosomal-associated transmembrane protein 4A [Salmo salar] 640 689774 1.4E-04 -0.3 gi_209154272, ACI33368.1, Serine/threonine-protein kinase PINK1, mitochondrial precursor [Salmo 97 6.18E-170 525 salar] 641 380658 2.3E-02 -0.3 gi_160333903, NP_001103941.1, alpha-parvin [Danio rerio] >gi_292616452, XP_002663041.1, 97 < 1.0E-180 642 PREDICTED: alpha-parvin-like [Danio rerio] >gi_134024868, AAI34925.1, Zgc:162217 protein [Danio rerio] 642 273238 5.8E-05 -0.3 gi_125821751, XP_691291.2, PREDICTED: solute carrier family 12 member 4 [Danio rerio] 96 < 1.0E-180 1651 643 119029 1.6E-06 -0.3 gi_116235426, NP_001070719.1, olfactomedin-like protein 3A precursor [Danio rerio] >gi_221272405, 91 < 1.0E-180 602 Q29RB4.2, OLF3A_DANRE RecName: Full=Olfactomedin-like protein 3A; Flags: Precursor >gi_115528152, AAI24743.1, Olfactomedin-like 3 [Danio rerio]

644 403135 2.2E-04 -0.4 gi_76153175, AAX24831.2, SJCHGC03009 protein [Schistosoma japonicum] 88 8.59E-58 198 645 318345 2.0E-03 -0.4 gi_68437543, XP_691101.1, PREDICTED: TBC1 domain family member 10A [Danio rerio] 83 3.93E-116 382 646 5926 1.0E-02 -0.4 gi_62955467, NP_001017747.1, coenzyme Q-binding protein COQ10 homolog B, mitochondrial 89 1.49E-129 403 precursor [Danio rerio] >gi_82178112, Q567E6.1, CQ10B_DANRE RecName: Full=Coenzyme Q-binding protein COQ10 homolog B, mitochondrial; Flags: Precursor >gi_62202676, AAH93204.1, Coenzyme Q10 homolog B (S. cerevisiae) [Danio rerio]

647 344591 4.2E-02 -0.4 gi_53933266, NP_001005603.1, chorion-specific transcription factor GCMb [Danio rerio] >gi_51773769, 85 < 1.0E-180 808 BAD38884.1, GCMb [Danio rerio] >gi_52854057, AAU88140.1, glial cell missing-like protein [Danio rerio] >gi_190339398, AAI62304.1, Glial cells missing homolog 2 (Drosophila) [Danio rerio]

648 51246 2.3E-02 -0.4 gi_47227433, CAG04581.1, unnamed protein product [Tetraodon nigroviridis] 83 1.03E-96 319 649 149557 7.6E-04 -0.4 gi_47212148, CAF94341.1, unnamed protein product [Tetraodon nigroviridis] 74 1.60E-31 135 650 662639 1.3E-04 -0.4 gi_410914622, XP_003970786.1, PREDICTED: endothelial cell-selective adhesion molecule-like 78 8.43E-115 309 [Takifugu rubripes] 651 315759 1.3E-10 -0.4 gi_410913813, XP_003970383.1, PREDICTED: large neutral amino acids transporter small subunit 3-like 84 < 1.0E-180 801 [Takifugu rubripes] 652 203524 3.9E-02 -0.4 gi_410908973, XP_003967965.1, PREDICTED: pleckstrin homology-like domain family B member 1-like 74 < 1.0E-180 790 [Takifugu rubripes] 653 743408 2.4E-02 -0.4 gi_410898529, XP_003962750.1, PREDICTED: 5'-nucleotidase-like [Takifugu rubripes] 86 < 1.0E-180 623 654 650285 2.5E-03 -0.4 gi_356640259, NP_001239283.1, CD99 molecule [Salmo salar] >gi_209155576, ACI34020.1, CD99 56 9.53E-04 49 antigen precursor [Salmo salar] 655 394929 3.3E-03 -0.4 gi_348535218, XP_003455098.1, PREDICTED: arrestin domain-containing protein 1-like, partial 76 8.94E-129 416 [Oreochromis niloticus] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 656 26670 1.0E-03 -0.4 gi_348533377, XP_003454182.1, PREDICTED: aspartate aminotransferase, cytoplasmic-like 93 < 1.0E-180 751 [Oreochromis niloticus] 657 161904 6.6E-06 -0.4 gi_348531032, XP_003453014.1, PREDICTED: sushi-repeat-containing protein SRPX-like [Oreochromis 87 < 1.0E-180 668 niloticus] 658 100832 1.5E-02 -0.4 gi_348526696, XP_003450855.1, PREDICTED: rho-related GTP-binding protein RhoU-like [Oreochromis 95 6.82E-137 422 niloticus] 659 9268 8.2E-03 -0.4 gi_348525653, XP_003450336.1, PREDICTED: transmembrane protein 106B-like [Oreochromis 91 2.32E-117 366 niloticus] 660 545816 2.7E-02 -0.4 gi_348521256, XP_003448142.1, PREDICTED: netrin-4 [Oreochromis niloticus] 87 < 1.0E-180 1042 661 14004 1.4E-04 -0.4 gi_348519687, XP_003447361.1, PREDICTED: homeobox protein Dlx2a [Oreochromis niloticus] 94 1.17E-147 438 662 80457 2.6E-02 -0.4 gi_348517704, XP_003446373.1, PREDICTED: scavenger receptor class A member 3-like [Oreochromis 84 < 1.0E-180 589 niloticus] 663 664956 1.8E-02 -0.4 gi_348515545, XP_003445300.1, PREDICTED: heparan sulfate glucosamine 3-O-sulfotransferase 1-like 91 1.44E-180 527 [Oreochromis niloticus] 664 556419 4.7E-04 -0.4 gi_348511847, XP_003443455.1, PREDICTED: protein FAM198B-like [Oreochromis niloticus] 73 < 1.0E-180 581 665 46826 1.4E-05 -0.4 gi_348504297, XP_003439698.1, PREDICTED: hematopoietic SH2 domain-containing protein homolog 52 4.36E-60 217 [Oreochromis niloticus] 666 306372 5.7E-05 -0.4 gi_348503149, XP_003439128.1, PREDICTED: integrin alpha-5-like [Oreochromis niloticus] 88 < 1.0E-180 1597 667 330041 9.0E-03 -0.4 gi_348501804, XP_003438459.1, PREDICTED: LOW QUALITY PROTEIN: protein FAM149B1-like 78 4.04E-138 455 [Oreochromis niloticus] 668 49146 4.2E-03 -0.4 gi_348501730, XP_003438422.1, PREDICTED: SH3 and PX domain-containing protein 2A-like 86 < 1.0E-180 1482 [Oreochromis niloticus] 669 575924 1.4E-02 -0.4 gi_317419931, CBN81967.1, GRAM domain-containing protein 1C, partial [Dicentrarchus labrax] 80 < 1.0E-180 562 670 319022 5.6E-03 -0.4 gi_317418700, CBN80738.1, Plakophilin-1 [Dicentrarchus labrax] 78 < 1.0E-180 594 671 184147 2.0E-02 -0.4 gi_299523039, NP_001177414.1, cullin-3 [Danio rerio] >gi_190340239, AAI63249.1, Cullin 3 [Danio 99 < 1.0E-180 739 rerio] 672 70777 4.9E-03 -0.4 gi_297692306, XP_002823503.1, PREDICTED: leucine-rich repeats and immunoglobulin-like domains 3 88 < 1.0E-180 765 isoform 2 [Pongo abelii] 673 185438 5.3E-06 -0.4 gi_259155192, NP_001158837.1, tyrosine phosphatase type IVA 1 [Salmo salar] >gi_223647656, 100 1.48E-117 361 ACN10586.1, tyrosine phosphatase type IVA 1 [Salmo salar] 674 250130 4.3E-11 -0.4 gi_238231799, NP_001154086.1, Superoxide dismutase [Oncorhynchus mykiss] >gi_225703940, 94 6.94E-96 292 ACO07816.1, Superoxide dismutase [Oncorhynchus mykiss] 675 394725 4.4E-04 -0.4 gi_225716592, ACO14142.1, Thioredoxin domain-containing protein 14 precursor [Esox lucius] 90 6.51E-160 504 676 513331 2.2E-03 -0.4 gi_225705168, ACO08430.1, ATP synthase coupling factor 6, mitochondrial precursor [Oncorhynchus 98 6.11E-27 117 mykiss] 677 35217 7.3E-03 -0.4 gi_224064163, XP_002188183.1, PREDICTED: hypothetical protein [Taeniopygia guttata] 77 4.81E-147 458 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 678 123497 3.2E-02 -0.4 gi_223648734, ACN11125.1, Elongation factor 2 [Salmo salar] 100 < 1.0E-180 1693 679 8218 2.3E-11 -0.4 gi_219130271, XP_002185292.1, probable cell surface glycoprotein [Phaeodactylum tricornutum CCAP 69 2.25E-22 112 1055/1] >gi_217403207, EEC43161.1, probable cell surface glycoprotein [Phaeodactylum tricornutum CCAP 1055/1] 680 614553 2.8E-09 -0.4 gi_213514254, NP_001133279.1, Nuclear factor erythroid 2-related factor 2 [Salmo salar] 97 < 1.0E-180 952 >gi_209148909, ACI32961.1, Nuclear factor erythroid 2-related factor 2 [Salmo salar] 681 359554 2.2E-02 -0.4 gi_213513646, NP_001133813.1, Sequestosome-1 [Salmo salar] >gi_209155416, ACI33940.1, 94 1.45E-180 532 Sequestosome-1 [Salmo salar] 682 672473 2.6E-03 -0.4 gi_213513636, NP_001133904.1, inositol hexaphosphate kinase 2 [Salmo salar] >gi_209155764, 97 < 1.0E-180 623 ACI34114.1, Inositol hexakisphosphate kinase 2 [Salmo salar] 683 116566 6.9E-05 -0.4 gi_213513097, NP_001133448.1, Alkaline phosphatase [Salmo salar] >gi_209154044, ACI33254.1, 95 < 1.0E-180 975 Alkaline phosphatase [Salmo salar] 684 282640 5.3E-04 -0.4 gi_213513069, NP_001133845.1, katanin p60 ATPase-containing subunit A1 [Salmo salar] 98 < 1.0E-180 803 >gi_238690595, B5X3X5.1, KTNA1_SALSA RecName: Full=Katanin p60 ATPase-containing subunit A1; Short=Katanin p60 subunit A1; AltName: Full=p60 katanin >gi_209155548, ACI34006.1, Katanin p60 ATPase-containing subunit [Salmo salar]

685 313793 1.1E-02 -0.4 gi_213512793, NP_001133527.1, CDC-like kinase 4 [Salmo salar] >gi_209154358, ACI33411.1, Dual 97 < 1.0E-180 651 specificity protein kinase CLK4 [Salmo salar] 686 410483 5.1E-02 -0.4 gi_213512615, NP_001133415.1, serine/threonine-protein kinase Sgk2 [Salmo salar] >gi_209153918, 100 2.03E-67 231 ACI33191.1, Serine/threonine-protein kinase Sgk2 [Salmo salar] 687 390426 9.1E-04 -0.4 gi_213511743, NP_001134775.1, microfibrillar-associated protein 2 precursor [Salmo salar] 87 3.78E-57 199 >gi_209735924, ACI68831.1, Microfibrillar-associated protein 2 precursor [Salmo salar] >gi_303666592, ADM16235.1, Microfibrillar-associated protein 2 precursor [Salmo salar]

688 412291 3.7E-02 -0.4 gi_213511226, NP_001133826.1, Lysosomal-associated transmembrane protein 4A [Salmo salar] 82 1.15E-68 238 >gi_209155470, ACI33967.1, Lysosomal-associated transmembrane protein 4A [Salmo salar] 689 32594 1.4E-04 -0.4 gi_213511022, NP_001133464.1, Para-nitrobenzyl esterase [Salmo salar] >gi_209154106, ACI33285.1, 99 < 1.0E-180 804 Para-nitrobenzyl esterase [Salmo salar] 690 646034 5.6E-03 -0.4 gi_213510780, NP_001134684.1, Glutamine synthetase [Salmo salar] >gi_209735226, ACI68482.1, 99 < 1.0E-180 732 Glutamine synthetase [Salmo salar] >gi_223647136, ACN10326.1, Glutamine synthetase [Salmo salar] >gi_223647518, ACN10517.1, Glutamine synthetase [Salmo salar] >gi_223673009, ACN12686.1, Glutamine synthetase [Salmo salar]

691 646012 2.5E-02 -0.4 gi_213510780, NP_001134684.1, Glutamine synthetase [Salmo salar] >gi_209735226, ACI68482.1, 99 < 1.0E-180 732 Glutamine synthetase [Salmo salar] >gi_223647136, ACN10326.1, Glutamine synthetase [Salmo salar] >gi_223647518, ACN10517.1, Glutamine synthetase [Salmo salar] >gi_223673009, ACN12686.1, Glutamine synthetase [Salmo salar]

692 387411 2.4E-05 -0.4 gi_189535788, XP_001921043.1, PREDICTED: tensin-like C1 domain-containing phosphatase-like 77 < 1.0E-180 1250 [Danio rerio] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 693 133198 1.3E-06 -0.4 gi_185135231, NP_001118134.1, Rhesus blood group family type B glycoprotein [Oncorhynchus mykiss] 99 < 1.0E-180 698 >gi_126542104, ABO16485.1, RhBGa [Oncorhynchus mykiss] >gi_126542106, ABO16486.1, RhBGb [Oncorhynchus mykiss]

694 662044 1.1E-03 -0.4 gi_185134049, NP_001117742.1, SOX9 alpha2 [Oncorhynchus mykiss] >gi_12003242, AAG43497.1, 98 < 1.0E-180 896 AF209872_1 SOX9 alpha2 [Oncorhynchus mykiss] 695 540560 < 1.0E-15 -0.4 gi_185132216, NP_001117192.1, hematological and neurological expressed 1 [Salmo salar] 99 5.86E-77 245 >gi_54150948, DAA05615.1, TPA_inf: HN1 protein [Salmo salar] 696 250992 1.3E-04 -0.4 gi_167555043, NP_001107898.1, E3 ubiquitin-protein ligase SMURF2 [Danio rerio] >gi_218547424, 93 < 1.0E-180 1456 A9JRZ0.1, SMUF2_DANRE RecName: Full=E3 ubiquitin-protein ligase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2 >gi_161611503, AAI55850.1, Smurf2 protein [Danio rerio]

697 218105 4.8E-03 -0.4 gi_157743316, NP_001099077.1, short-chain dehydrogenase/reductase 10 precursor [Danio rerio] 84 1.87E-130 393 >gi_183583550, NP_956617.2, hydroxysteroid 11-beta-dehydrogenase 1-like protein precursor [Danio rerio] >gi_82202701, Q6PUF3.1, DHI1L_DANRE RecName: Full=Hydroxysteroid 11-beta-dehydrogenase 1-like protein; AltName: Full=11-beta-hydroxysteroid dehydrogenase type 3; Short=11-DH3; Short=11- beta-HSD3; Flags: Precursor >gi_46361406, AAS89256.1, 11-beta-hydroxysteroid dehydrogenase type 3 [Danio rerio] >gi_47938832, AAH71452.1, Hsd11b3 protein [Danio rerio] >gi_94733978, CAK11206.1, hydroxysteroid (11-beta) dehydrogenase 3 [Danio rerio] >gi_157423427, AAI53594.1, LOC100006793 protein [Danio rerio] 698 538056 3.5E-03 -0.4 gi_113676876, NP_001038608.1, neuron navigator 3 [Danio rerio] >gi_82079546, Q5TZ18.1, 86 < 1.0E-180 1635 NAV3_DANRE RecName: Full=Neuron navigator 3 >gi_55251043, CAH69140.1, novel protein similar to vertebrate neuron navigator family [Danio rerio] >gi_56207575, CAI20640.1, novel protein similar to vertebrate neuron navigator family [Danio rerio]

699 57349 1.2E-06 -0.5 gi_54289506, AAS78629.1, gamma-glutamyl carboxylase [Sparus aurata] 88 < 1.0E-180 1225 700 762646 1.4E-04 -0.5 gi_47209751, CAF93869.1, unnamed protein product [Tetraodon nigroviridis] 95 < 1.0E-180 742 701 634898 4.3E-03 -0.5 gi_47086905, NP_997727.1, 3'-phosphoadenosine 5'-phosphosulfate synthase 2b [Danio rerio] 93 < 1.0E-180 1121 >gi_28502934, AAH47190.1, 3'-phosphoadenosine 5'-phosphosulfate synthase 2 [Danio rerio] >gi_46250238, AAH68346.1, 3'-phosphoadenosine 5'-phosphosulfate synthase 2 [Danio rerio]

702 17920 < 1.0E-15 -0.5 gi_410920409, XP_003973676.1, PREDICTED: matrix-remodeling-associated protein 8-like [Takifugu 80 2.05E-155 484 rubripes] 703 731779 1.1E-03 -0.5 gi_410916887, XP_003971918.1, PREDICTED: sestrin-1-like [Takifugu rubripes] 93 < 1.0E-180 860 704 154782 6.8E-04 -0.5 gi_410898313, XP_003962642.1, PREDICTED: cysteine-rich motor neuron 1 protein-like [Takifugu 87 < 1.0E-180 1542 rubripes] 705 474416 6.6E-07 -0.5 gi_37678182, AAP49009.1, transposase [Rana pipiens] >gi_39753972, DAA01561.1, TPA_exp: 61 1.97E-40 162 transposase [Rana pipiens] 706 73028 5.0E-04 -0.5 gi_348542553, XP_003458749.1, PREDICTED: PHD finger protein 23B-like [Oreochromis niloticus] 80 2.18E-110 362 707 26896 4.4E-03 -0.5 gi_348537912, XP_003456436.1, PREDICTED: phospholipase D3-like [Oreochromis niloticus] 87 < 1.0E-180 669 708 280230 1.7E-08 -0.5 gi_348526774, XP_003450894.1, PREDICTED: transmembrane protease serine 13-like [Oreochromis 72 < 1.0E-180 574 niloticus] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 709 44744 1.2E-03 -0.5 gi_348524747, XP_003449884.1, PREDICTED: alkaline ceramidase 3-like [Oreochromis niloticus] 92 6.24E-154 475 710 587818 2.3E-02 -0.5 gi_348524384, XP_003449703.1, PREDICTED: forkhead box protein D1-like [Oreochromis niloticus] 93 4.15E-114 228 711 563042 3.7E-02 -0.5 gi_348520014, XP_003447524.1, PREDICTED: neuropilin-1a-like [Oreochromis niloticus] 80 < 1.0E-180 1325 712 241842 1.9E-12 -0.5 gi_348519244, XP_003447141.1, PREDICTED: EIN3-binding F-box protein 1-like [Oreochromis niloticus] 78 < 1.0E-180 732 713 699980 2.0E-05 -0.5 gi_348519214, XP_003447126.1, PREDICTED: cat eye syndrome critical region protein 5 homolog 85 < 1.0E-180 679 [Oreochromis niloticus] 714 364458 1.5E-04 -0.5 gi_348515153, XP_003445104.1, PREDICTED: A disintegrin and metalloproteinase with thrombospondin 92 < 1.0E-180 1186 motifs 10 [Oreochromis niloticus] 715 307773 6.7E-04 -0.5 gi_348514413, XP_003444735.1, PREDICTED: janus kinase and microtubule-interacting protein 3-like 90 1.06E-180 562 [Oreochromis niloticus] 716 214875 1.4E-05 -0.5 gi_348511360, XP_003443212.1, PREDICTED: serine/threonine-protein kinase N2-like [Oreochromis 90 < 1.0E-180 779 niloticus] 717 28772 9.3E-03 -0.5 gi_348509720, XP_003442395.1, PREDICTED: E3 ubiquitin-protein ligase NEDD4-like [Oreochromis 86 < 1.0E-180 1466 niloticus] 718 663236 8.7E-05 -0.5 gi_348508454, XP_003441769.1, PREDICTED: MDS1 and EVI1 complex locus protein EVI1-like 88 < 1.0E-180 718 [Oreochromis niloticus] 719 121813 5.3E-03 -0.5 gi_348506906, XP_003440998.1, PREDICTED: junctional adhesion molecule B-like [Oreochromis 80 8.93E-15 87 niloticus] 720 332474 3.0E-04 -0.5 gi_348504542, XP_003439820.1, PREDICTED: palladin [Oreochromis niloticus] 97 < 1.0E-180 794 721 402519 3.9E-03 -0.5 gi_348503109, XP_003439109.1, PREDICTED: protein FAM132A-like [Oreochromis niloticus] 92 2.08E-151 472 722 124750 2.5E-02 -0.5 gi_348502291, XP_003438701.1, PREDICTED: hypothetical protein LOC100710991 [Oreochromis 66 3.74E-42 161 niloticus] 723 45033 1.4E-03 -0.5 gi_348501360, XP_003438238.1, PREDICTED: fizzy-related protein homolog isoform 1 [Oreochromis 97 1.25E-127 412 niloticus] 724 70033 2.9E-02 -0.5 gi_348501025, XP_003438071.1, PREDICTED: cell division cycle 7-related protein kinase-like 71 7.06E-114 367 [Oreochromis niloticus] 725 540442 5.1E-02 -0.5 gi_348500977, XP_003438047.1, PREDICTED: solute carrier family 25 member 36-A-like [Oreochromis 94 5.25E-96 325 niloticus] 726 333379 3.5E-02 -0.5 gi_345320086, XP_001520292.2, PREDICTED: vascular cell adhesion protein 1-like [Ornithorhynchus 45 1.57E-20 107 anatinus] 727 346384 3.8E-12 -0.5 gi_326681053, XP_003201702.1, PREDICTED: e3 ubiquitin-protein ligase Itchy-like [Danio rerio] 94 < 1.0E-180 827 728 526754 2.0E-02 -0.5 gi_326669672, XP_003199061.1, PREDICTED: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N- 95 < 1.0E-180 1149 acetylgalactosaminyltransferase-like 4b [Danio rerio] 729 299146 1.1E-02 -0.5 gi_326667865, XP_003198687.1, PREDICTED: serine/threonine-protein kinase N2 [Danio rerio] 86 < 1.0E-180 695 730 44979 1.7E-03 -0.5 gi_317418929, CBN80967.1, PDZ domain-containing RING finger protein 3 [Dicentrarchus labrax] 91 < 1.0E-180 1672 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 731 230355 4.6E-02 -0.5 gi_301610714, XP_002934892.1, PREDICTED: proteinase-activated receptor 1-like [Xenopus (Silurana) 62 3.17E-66 168 tropicalis] 732 736870 5.4E-02 -0.5 gi_291190256, NP_001167219.1, Hepatic leukemia factor [Salmo salar] >gi_223648736, ACN11126.1, 78 3.35E-100 330 Hepatic leukemia factor [Salmo salar] 733 502868 6.4E-04 -0.5 gi_291190216, NP_001167208.1, Zinc finger protein 710 [Salmo salar] >gi_223648678, ACN11097.1, 97 < 1.0E-180 1253 Zinc finger protein 710 [Salmo salar] 734 474469 5.4E-05 -0.5 gi_225707736, ACO09714.1, Tumor necrosis factor receptor superfamily member 19 precursor [Osmerus 79 5.26E-175 527 mordax] 735 726670 1.2E-03 -0.5 gi_225705522, ACO08607.1, NEDD4 family-interacting protein 1 [Oncorhynchus mykiss] 94 1.59E-93 293 736 390462 1.2E-02 -0.5 gi_221219254, ACM08288.1, Microfibrillar-associated protein 2 precursor [Salmo salar] 97 3.37E-67 224 737 132348 9.2E-03 -0.5 gi_213514164, NP_001134558.1, Apoptosis regulator Bcl-X [Salmo salar] >gi_209734268, ACI68003.1, 92 1.99E-133 410 Apoptosis regulator Bcl-X [Salmo salar] 738 539866 5.9E-04 -0.5 gi_213513774, NP_001133337.1, Calponin-3 [Salmo salar] >gi_209150926, ACI33050.1, Calponin-3 99 < 1.0E-180 628 [Salmo salar] 739 672486 6.9E-08 -0.5 gi_213513636, NP_001133904.1, inositol hexaphosphate kinase 2 [Salmo salar] >gi_209155764, 98 < 1.0E-180 683 ACI34114.1, Inositol hexakisphosphate kinase 2 [Salmo salar] 740 239735 2.0E-05 -0.5 gi_213513503, NP_001135315.1, Metalloproteinase inhibitor 3 precursor [Salmo salar] >gi_209732272, 95 7.61E-120 389 ACI67005.1, Metalloproteinase inhibitor 3 precursor [Salmo salar] 741 146638 1.8E-02 -0.5 gi_213510848, NP_001133517.1, Guanine nucleotide-binding protein GI/GS/GO subunit gamma-12 97 4.89E-36 142 [Salmo salar] >gi_259089299, NP_001158687.1, Guanine nucleotide-binding protein GI/GS/GO gamma- 12 subunit [Oncorhynchus mykiss] >gi_209154322, ACI33393.1, Guanine nucleotide-binding protein GI/GS/GO subunit gamma-12 precursor [Salmo salar] >gi_225705872, ACO08782.1, Guanine nucleotide-binding protein GI/GS/GO gamma-12 subunit precursor [Oncorhynchus mykiss]

742 504509 1.1E-03 -0.5 gi_209962463, ACJ02099.1, FGF2 [Salmo salar] 96 3.02E-06 50 743 142321 6.4E-04 -0.5 gi_209149990, ACI33002.1, Follistatin-related protein 1 precursor [Salmo salar] 98 < 1.0E-180 565 744 546215 5.6E-04 -0.5 gi_18858661, NP_571871.1, focal adhesion kinase 1 [Danio rerio] >gi_13569579, AAK31154.1, 89 < 1.0E-180 1657 AF348085_1 focal adhesion kinase 1a [Danio rerio] 745 12760 6.0E-07 -0.5 gi_185134552, NP_001117921.1, latent transforming growth factor binding protein precursor 84 < 1.0E-180 1541 [Oncorhynchus mykiss] >gi_27549564, AAO17057.1, latent transforming growth factor binding protein [Oncorhynchus mykiss] 746 416116 9.0E-09 -0.5 gi_150378445, NP_001092900.1, protein asteroid homolog 1 [Danio rerio] >gi_123889254, Q1LYL8.1, 60 2.34E-149 477 ASTE1_DANRE RecName: Full=Protein asteroid homolog 1 >gi_94732441, CAK05130.1, novel protein similar to human HT001 protein (HT001) [Danio rerio] >gi_190339910, AAI63487.1, Asteroid homolog 1 (Drosophila) [Danio rerio] >gi_190339914, AAI63493.1, Asteroid homolog 1 (Drosophila) [Danio rerio]

747 108617 6.2E-03 -0.5 gi_146147385, ABQ01988.1, reverse transcriptase-like protein [Salmo salar] >gi_148362149, 100 9.09E-13 74 ABQ59672.1, reverse transcriptase-like protein [Salmo salar] >gi_260182170, ACX35599.1, reverse transcriptase-like protein [Salmo salar]

748 11131 3.8E-03 -0.5 gi_126632452, CAM56314.1, CNDP dipeptidase 2 (metallopeptidase M20 family) [Danio rerio] 91 < 1.0E-180 659 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 749 480716 4.3E-08 -0.6 gi_86285723, ABC94584.1, protein phosphatase 1 catalytic subunit beta isoform [Scophthalmus 100 < 1.0E-180 621 maximus] 750 729477 3.8E-04 -0.6 gi_60688354, AAH91673.1, Lamb1 protein, partial [Danio rerio] 88 3.67E-162 496 751 100365 1.4E-04 -0.6 gi_51556245, NP_991292.1, nuclear receptor subfamily 1 group D member 1 [Danio rerio] 75 < 1.0E-180 712 >gi_37362256, AAQ91256.1, nuclear receptor subfamily 1, group D, member 1 [Danio rerio] 752 288655 1.4E-02 -0.6 gi_410922156, XP_003974549.1, PREDICTED: carnitine O-acetyltransferase-like [Takifugu rubripes] 89 < 1.0E-180 957 753 330241 4.8E-02 -0.6 gi_410917418, XP_003972183.1, PREDICTED: tripartite motif-containing protein 16-like [Takifugu 92 8.45E-156 480 rubripes] 754 306963 4.3E-03 -0.6 gi_410911122, XP_003969039.1, PREDICTED: oxysterol-binding protein-related protein 3-like [Takifugu 85 < 1.0E-180 1092 rubripes] 755 468655 3.1E-03 -0.6 gi_410907313, XP_003967136.1, PREDICTED: aryl hydrocarbon receptor nuclear translocator-like 94 < 1.0E-180 1118 protein 1-like [Takifugu rubripes] 756 493764 4.1E-03 -0.6 gi_410906971, XP_003966965.1, PREDICTED: rho guanine nucleotide exchange factor 15-like [Takifugu 69 < 1.0E-180 980 rubripes] 757 51283 1.4E-02 -0.6 gi_410905783, XP_003966371.1, PREDICTED: prolyl 3-hydroxylase 3-like [Takifugu rubripes] 83 < 1.0E-180 597 758 347834 2.5E-08 -0.6 gi_410904923, XP_003965941.1, PREDICTED: NADP-dependent malic enzyme, mitochondrial-like 94 < 1.0E-180 767 [Takifugu rubripes] 759 510712 3.5E-02 -0.6 gi_410903860, XP_003965411.1, PREDICTED: claudin-5-like [Takifugu rubripes] >gi_49333497, 89 3.76E-101 317 AAT64087.1, claudin 5b [Takifugu rubripes] 760 278063 2.3E-02 -0.6 gi_410902831, XP_003964897.1, PREDICTED: UNC93-like protein MFSD11-like [Takifugu rubripes] 88 3.42E-176 466 761 287478 1.1E-03 -0.6 gi_395731302, XP_002811632.2, PREDICTED: PR domain zinc finger protein 16 [Pongo abelii] 77 1.72E-05 56 762 692831 8.3E-05 -0.6 gi_395543089, XP_003773455.1, PREDICTED: actin filament-associated protein 1 isoform 1 83 < 1.0E-180 570 [Sarcophilus harrisii] 763 213154 2.6E-11 -0.6 gi_38541948, AAH62835.1, Platelet-derived growth factor receptor-like [Danio rerio] 85 < 1.0E-180 575 764 596074 1.5E-06 -0.6 gi_37681867, AAQ97811.1, insulin receptor tyrosine kinase substrate [Danio rerio] 82 7.54E-149 472 765 335246 7.2E-04 -0.6 gi_374722810, AEZ68569.1, EPS8L1a-like protein [Osmerus mordax] 73 1.56E-71 260 766 403036 1.2E-04 -0.6 gi_350579164, XP_001926069.4, PREDICTED: angiopoietin-1 receptor, partial [Sus scrofa] 81 < 1.0E-180 827 767 200285 3.2E-02 -0.6 gi_348535210, XP_003455094.1, PREDICTED: protein CYR61-like [Oreochromis niloticus] 77 3.82E-162 494 768 546453 8.8E-06 -0.6 gi_348531124, XP_003453060.1, PREDICTED: laminin subunit alpha-4 [Oreochromis niloticus] 86 < 1.0E-180 1834 769 139694 1.8E-13 -0.6 gi_348529574, XP_003452288.1, PREDICTED: spermine oxidase-like [Oreochromis niloticus] 89 < 1.0E-180 917 770 17469 2.0E-04 -0.6 gi_348526552, XP_003450783.1, PREDICTED: prickle-like protein 1-like [Oreochromis niloticus] 71 < 1.0E-180 995 771 259009 1.1E-02 -0.6 gi_348521017, XP_003448023.1, PREDICTED: secernin-2-like [Oreochromis niloticus] 90 < 1.0E-180 719 772 522399 2.8E-02 -0.6 gi_348519867, XP_003447451.1, PREDICTED: cordon-bleu protein-like 1-like [Oreochromis niloticus] 60 < 1.0E-180 684 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 773 592585 2.1E-02 -0.6 gi_348515369, XP_003445212.1, PREDICTED: phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 82 < 1.0E-180 888 2B-like [Oreochromis niloticus] 774 20666 2.9E-02 -0.6 gi_348513995, XP_003444526.1, PREDICTED: doublesex- and mab-3-related transcription factor 2 74 < 1.0E-180 565 [Oreochromis niloticus] 775 499681 1.6E-02 -0.6 gi_348510371, XP_003442719.1, PREDICTED: GATA-binding factor 2-like [Oreochromis niloticus] 94 8.51E-179 530 776 663199 1.1E-02 -0.6 gi_348508454, XP_003441769.1, PREDICTED: MDS1 and EVI1 complex locus protein EVI1-like 88 < 1.0E-180 717 [Oreochromis niloticus] 777 77471 8.9E-06 -0.6 gi_348508032, XP_003441559.1, PREDICTED: lumican-like [Oreochromis niloticus] 82 1.58E-123 382 778 551832 3.4E-03 -0.6 gi_348504136, XP_003439618.1, PREDICTED: dickkopf-related protein 3-like [Oreochromis niloticus] 73 5.12E-106 330 779 641983 3.2E-12 -0.6 gi_348502745, XP_003438928.1, PREDICTED: EMILIN-3-like [Oreochromis niloticus] 83 < 1.0E-180 1118 780 79724 2.5E-05 -0.6 gi_348500252, XP_003437687.1, PREDICTED: lysyl oxidase homolog 1-like [Oreochromis niloticus] 76 < 1.0E-180 686 781 256739 1.6E-06 -0.6 gi_326679186, XP_003201256.1, PREDICTED: basement membrane-specific heparan sulfate 87 < 1.0E-180 1869 proteoglycan core protein [Danio rerio] 782 751931 3.5E-07 -0.6 gi_326670654, XP_002663415.2, PREDICTED: collagen alpha-3(VI) chain [Danio rerio] 97 1.31E-25 91 783 371066 1.3E-02 -0.6 gi_326670523, XP_687811.5, PREDICTED: collagen alpha-2(IV) chain, partial [Danio rerio] 68 < 1.0E-180 1559 784 589201 3.3E-07 -0.6 gi_326666238, XP_684101.5, PREDICTED: calmodulin-regulated spectrin-associated protein 3 [Danio 70 < 1.0E-180 1111 rerio] 785 499735 1.5E-05 -0.6 gi_317419025, CBN81063.1, GATA-binding factor 2 [Dicentrarchus labrax] 93 1.97E-57 206 786 91424 2.3E-05 -0.6 gi_292622746, XP_002665090.1, PREDICTED: v-set and immunoglobulin domain-containing protein 10- 67 5.32E-144 459 like [Danio rerio] 787 30578 1.5E-03 -0.6 gi_292610789, XP_001920672.2, PREDICTED: nuclear receptor-binding protein 2 [Danio rerio] 90 < 1.0E-180 780 788 94261 1.2E-02 -0.6 gi_226443073, NP_001140027.1, Glycophorin-C [Salmo salar] >gi_221221284, ACM09303.1, 83 8.25E-33 135 Glycophorin-C [Salmo salar] 789 412562 1.8E-03 -0.6 gi_225707214, ACO09453.1, Tumor necrosis factor receptor superfamily member 14 precursor [Osmerus 56 2.00E-40 158 mordax] 790 329094 3.3E-11 -0.6 gi_225706062, ACO08877.1, Tumor necrosis factor receptor superfamily member 14 precursor [Osmerus 65 4.34E-63 226 mordax] 791 297284 2.6E-02 -0.6 gi_223649434, ACN11475.1, Transposable element Tcb1 transposase [Salmo salar] 63 4.42E-17 69 792 606958 1.7E-10 -0.6 gi_213513790, NP_001133302.1, Glycogenin-1 [Salmo salar] >gi_209149883, ACI32996.1, Glycogenin- 96 < 1.0E-180 660 1 [Salmo salar] 793 359540 6.3E-06 -0.6 gi_213513646, NP_001133813.1, Sequestosome-1 [Salmo salar] >gi_209155416, ACI33940.1, 85 3.19E-176 527 Sequestosome-1 [Salmo salar] 794 626480 2.1E-05 -0.6 gi_213511731, NP_001133838.1, Tumor protein D53 homolog [Salmo salar] >gi_209155528, 97 1.65E-30 127 ACI33996.1, Tumor protein D53 homolog [Salmo salar] 795 626528 9.9E-04 -0.6 gi_213511731, NP_001133838.1, Tumor protein D53 homolog [Salmo salar] >gi_209155528, 99 4.27E-73 252 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score ACI33996.1, Tumor protein D53 homolog [Salmo salar]

796 18617 6.1E-04 -0.6 gi_209732964, ACI67351.1, Carbonyl reductase 1 [Salmo salar] >gi_303667581, ADM16272.1, 99 < 1.0E-180 554 Carbonyl reductase 1 [Salmo salar] 797 385498 2.0E-06 -0.6 gi_209730800, ACI66269.1, Glyceraldehyde-3-phosphate dehydrogenase [Salmo salar] 98 5.32E-135 423 798 208141 2.2E-03 -0.6 gi_209155900, ACI34182.1, Procollagen C-endopeptidase enhancer 1 precursor [Salmo salar] 94 < 1.0E-180 713 799 344685 6.7E-04 -0.6 gi_209155872, ACI34168.1, SPARC precursor [Salmo salar] >gi_209731568, ACI66653.1, SPARC 98 2.09E-173 519 precursor [Salmo salar] 800 344710 6.1E-05 -0.6 gi_197632219, ACH70833.1, secreted acidic cysteine rich glycoprotein [Salmo salar] >gi_209737738, 98 < 1.0E-180 571 ACI69738.1, SPARC precursor [Salmo salar] >gi_223647020, ACN10268.1, SPARC precursor [Salmo salar] >gi_223672885, ACN12624.1, SPARC precursor [Salmo salar]

801 706928 5.3E-02 -0.6 gi_194222045, XP_001496580.2, PREDICTED: collagen alpha-1(IV) chain [Equus caballus] 77 < 1.0E-180 523 802 709675 6.0E-11 -0.6 gi_189525193, XP_001923590.1, PREDICTED: prolargin [Danio rerio] 88 6.41E-177 520 803 407351 4.7E-08 -0.6 gi_185135951, NP_001118049.1, Rhcg1-a [Oncorhynchus mykiss] >gi_116008510, ABD92924.1, 98 1.18E-133 435 Rhcg1-a [Oncorhynchus mykiss] >gi_126542108, ABO16487.1, RhCG1-b [Oncorhynchus mykiss] 804 469042 6.0E-10 -0.6 gi_166092745, ABY82346.1, R-ras [Kryptolebias marmoratus] 99 4.14E-122 383 805 200533 6.4E-03 -0.6 gi_156713471, NP_001096145.1, partitioning defective 6 homolog beta [Danio rerio] >gi_150404786, 95 3.35E-150 473 ABR68551.1, partitioning defective 6 beta [Danio rerio] 806 726306 2.6E-05 -0.6 gi_126631270, AAI33139.1, Si:ch211-124k10.2 protein [Danio rerio] 79 < 1.0E-180 1144 807 711178 3.3E-02 -0.6 gi_124298401, ABN04301.1, cytoplasmatic polyadenylation element binding protein 2B [Danio rerio] 97 < 1.0E-180 707 808 227060 6.7E-06 -0.7 gi_71834306, NP_001025243.1, fibromodulin-like precursor [Danio rerio] >gi_66910340, AAH96939.1, 79 6.97E-138 437 Zgc:113456 [Danio rerio] >gi_182888920, AAI64382.1, Zgc:113456 protein [Danio rerio] 809 706537 1.7E-10 -0.7 gi_55925442, NP_001007292.1, 3-oxoacid CoA transferase 1a [Danio rerio] >gi_50603835, 92 < 1.0E-180 883 AAH78408.1, 3-oxoacid CoA transferase 1a [Danio rerio] >gi_159569846, CAP19426.1, 3-oxoacid CoA transferase 1 [Danio rerio] >gi_169154681, CAQ14272.1, 3-oxoacid CoA transferase 1 [Danio rerio] >gi_169154981, CAQ13456.1, 3-oxoacid CoA transferase 1 [Danio rerio] >gi_182891146, AAI63972.1, 3-oxoacid CoA transferase 1a [Danio rerio]

810 51228 1.2E-03 -0.7 gi_47227433, CAG04581.1, unnamed protein product [Tetraodon nigroviridis] 87 9.43E-97 319 811 31969 4.0E-05 -0.7 gi_47219243, CAG11705.1, unnamed protein product [Tetraodon nigroviridis] 55 9.01E-172 596 812 395814 1.0E-02 -0.7 gi_45259478, BAD12391.1, aggrecan [Danio rerio] 89 1.19E-106 355 813 643055 3.3E-03 -0.7 gi_413951841, AFW84490.1, hypothetical protein ZEAMMB73_332720, partial [Zea mays] 56 3.79E-14 80 814 63518 3.9E-03 -0.7 gi_410918474, XP_003972710.1, PREDICTED: oxysterol-binding protein-related protein 8-like [Takifugu 92 < 1.0E-180 660 rubripes] 815 538708 < 1.0E-15 -0.7 gi_410913537, XP_003970245.1, PREDICTED: uncharacterized protein LOC101072125 [Takifugu 68 < 1.0E-180 775 rubripes] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 816 115277 4.5E-05 -0.7 gi_410911578, XP_003969267.1, PREDICTED: uncharacterized protein LOC101067704 [Takifugu 73 5.18E-68 260 rubripes] 817 355244 2.9E-02 -0.7 gi_410905825, XP_003966392.1, PREDICTED: carcinoembryonic antigen-related cell adhesion molecule 59 4.37E-16 88 1-like [Takifugu rubripes] 818 79075 8.2E-03 -0.7 gi_410896244, XP_003961609.1, PREDICTED: LOW QUALITY PROTEIN: myosin phosphatase Rho- 68 < 1.0E-180 1119 interacting protein-like [Takifugu rubripes] 819 718453 1.5E-02 -0.7 gi_41055863, NP_956450.1, nuclear pore complex protein Nup155 [Danio rerio] >gi_28277555, 92 < 1.0E-180 1158 AAH45339.1, Nucleoporin 155 [Danio rerio] 820 743777 3.7E-03 -0.7 gi_380800079, AFE71915.1, plexin-A2 precursor, partial [Macaca mulatta] >gi_380800081, AFE71916.1, 97 < 1.0E-180 787 plexin-A2 precursor, partial [Macaca mulatta] 821 679490 2.3E-05 -0.7 gi_371902294, CAZ61327.1, CD34a molecule [Oncorhynchus mykiss] 65 1.49E-94 317 822 387364 8.2E-04 -0.7 gi_348541261, XP_003458105.1, PREDICTED: disco-interacting protein 2 homolog C-like [Oreochromis 97 < 1.0E-180 1361 niloticus] 823 643643 3.5E-02 -0.7 gi_348533430, XP_003454208.1, PREDICTED: heat shock protein beta-8-like [Oreochromis niloticus] 84 8.96E-105 331 824 542041 2.7E-03 -0.7 gi_348525705, XP_003450362.1, PREDICTED: multiple PDZ domain protein [Oreochromis niloticus] 76 < 1.0E-180 1676 825 53407 2.6E-02 -0.7 gi_348522905, XP_003448964.1, PREDICTED: collagen alpha-2(IX) chain-like [Oreochromis niloticus] 89 < 1.0E-180 609 826 169378 1.2E-02 -0.7 gi_348521268, XP_003448148.1, PREDICTED: laminin subunit beta-1-like [Oreochromis niloticus] 75 1.60E-103 242 827 545377 4.3E-10 -0.7 gi_348520919, XP_003447974.1, PREDICTED: kelch-like ECH-associated protein 1-like [Oreochromis 95 < 1.0E-180 1121 niloticus] 828 242030 3.6E-02 -0.7 gi_348519154, XP_003447096.1, PREDICTED: engulfment and cell motility protein 3-like [Oreochromis 93 < 1.0E-180 1163 niloticus] 829 656278 8.1E-03 -0.7 gi_348517797, XP_003446419.1, PREDICTED: potassium voltage-gated channel subfamily S member 87 < 1.0E-180 778 3-like [Oreochromis niloticus] 830 490364 3.0E-02 -0.7 gi_348517710, XP_003446376.1, PREDICTED: hypothetical protein LOC100706280 [Oreochromis 86 7.12E-127 427 niloticus] 831 699242 1.3E-02 -0.7 gi_348516711, XP_003445881.1, PREDICTED: forkhead box protein I1-ema-like [Oreochromis niloticus] 81 3.23E-131 424 832 529034 4.5E-03 -0.7 gi_348513571, XP_003444315.1, PREDICTED: bcl-2-related ovarian killer protein homolog A-like 93 6.18E-126 383 [Oreochromis niloticus] 833 10595 5.8E-05 -0.7 gi_348513526, XP_003444293.1, PREDICTED: podocan [Oreochromis niloticus] 88 < 1.0E-180 759 834 189079 3.7E-02 -0.7 gi_348512855, XP_003443958.1, PREDICTED: prickle-like protein 1-like [Oreochromis niloticus] 75 < 1.0E-180 658 835 280805 7.0E-04 -0.7 gi_348509418, XP_003442246.1, PREDICTED: KN motif and ankyrin repeat domain-containing protein 71 1.80E-176 558 2-like [Oreochromis niloticus] 836 304778 4.4E-03 -0.7 gi_348508436, XP_003441760.1, PREDICTED: hypothetical protein LOC100696699 [Oreochromis 84 3.10E-50 196 niloticus] 837 119398 1.4E-03 -0.7 gi_348508306, XP_003441695.1, PREDICTED: frizzled-4-like [Oreochromis niloticus] 94 < 1.0E-180 909 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 838 734888 < 1.0E-15 -0.7 gi_348508209, XP_003441647.1, PREDICTED: Friend leukemia integration 1 transcription factor isoform 95 < 1.0E-180 809 2 [Oreochromis niloticus] 839 620403 9.6E-08 -0.7 gi_326672295, XP_691455.4, PREDICTED: probable E3 ubiquitin-protein ligase HECTD2-like [Danio 94 < 1.0E-180 1237 rerio] 840 53715 2.5E-04 -0.7 gi_319918507, BAJ61837.1, aggrecan [Oncorhynchus keta] 96 < 1.0E-180 2220 841 227184 2.0E-03 -0.7 gi_317419841, CBN81877.1, Uncharacterized protein [Dicentrarchus labrax] 52 1.92E-22 104 842 694553 2.4E-04 -0.7 gi_24111232, NP_705929.1, serine/threonine-protein kinase receptor R3 precursor [Danio rerio] 83 < 1.0E-180 711 >gi_21427116, AAM53074.1, AF435024_1 serine/threonine kinase receptor [Danio rerio] >gi_21427118, AAM53075.1, AF435025_1 serine/threonine kinase receptor [Danio rerio]

843 137046 < 1.0E-15 -0.7 gi_238231807, NP_001154090.1, glutathione S-transferase omega 1 [Oncorhynchus mykiss] 98 2.61E-164 488 >gi_225703964, ACO07828.1, Glutathione transferase omega-1 [Oncorhynchus mykiss] 844 614884 3.7E-03 -0.7 gi_224587796, ACN58714.1, Myosin-VI [Salmo salar] 94 9.66E-117 357 845 242519 3.2E-05 -0.7 gi_223649434, ACN11475.1, Transposable element Tcb1 transposase [Salmo salar] 60 3.23E-72 211 846 239722 7.0E-03 -0.7 gi_223647586, ACN10551.1, Metalloproteinase inhibitor 3 precursor [Salmo salar] 100 5.24E-38 153 847 715142 1.7E-13 -0.7 gi_221222086, ACM09704.1, Metalloproteinase inhibitor 2 precursor [Salmo salar] >gi_223648072, 93 1.45E-102 337 ACN10794.1, Metalloproteinase inhibitor 2 precursor [Salmo salar] 848 664521 1.1E-05 -0.7 gi_221219646, ACM08484.1, Dehydrogenase/reductase SDR family member 13 precursor [Salmo salar] 99 2.79E-151 444 849 318373 4.3E-12 -0.7 gi_213515488, NP_001135274.1, Glutamate--cysteine ligase catalytic subunit [Salmo salar] 98 < 1.0E-180 1159 >gi_209155784, ACI34124.1, Glutamate--cysteine ligase catalytic subunit [Salmo salar] 850 203312 1.4E-02 -0.7 gi_213514842, NP_001133343.1, UDP-glucuronosyltransferase 2A2 [Salmo salar] >gi_209151120, 98 < 1.0E-180 973 ACI33061.1, UDP-glucuronosyltransferase 2A2 precursor [Salmo salar] 851 631516 3.7E-08 -0.7 gi_213512238, NP_001133443.1, Transforming growth factor beta-1-induced transcript 1 protein [Salmo 100 1.61E-92 319 salar] >gi_209154026, ACI33245.1, Transforming growth factor beta-1-induced transcript 1 protein [Salmo salar] 852 91333 7.1E-07 -0.7 gi_213512008, NP_001134237.1, protein sprouty homolog 4 [Salmo salar] >gi_209731720, ACI66729.1, 91 1.20E-139 409 SPRY domain-containing protein 4 [Salmo salar] 853 651570 2.6E-02 -0.7 gi_213511554, NP_001134915.1, FAM70B [Salmo salar] >gi_209737164, ACI69451.1, FAM70B [Salmo 92 5.07E-55 199 salar] 854 79315 3.7E-03 -0.7 gi_213511322, NP_001134543.1, Glutathione-requiring prostaglandin D synthase [Salmo salar] 70 8.33E-90 280 >gi_209734168, ACI67953.1, Glutathione-requiring prostaglandin D synthase [Salmo salar] 855 523057 1.8E-04 -0.7 gi_209155952, ACI34208.1, RNA-binding protein 4B [Salmo salar] 83 3.03E-150 474 856 1662 2.0E-02 -0.7 gi_189536156, XP_691607.3, PREDICTED: nuclear receptor subfamily 1 group D member 2 [Danio 75 < 1.0E-180 606 rerio] >gi_220678407, CAX12925.1, novel protein similar to H.sapeins NR1D2, nuclear receptor subfamily 1, group D, member 2 (NR1D2) [Danio rerio]

857 25409 6.6E-04 -0.7 gi_18858321, NP_571745.1, ATPase, Na+/K+ transporting, beta 3b polypeptide [Danio rerio] 85 3.45E-95 313 >gi_9837579, AAG00609.1, AF293369_1 Na,K-ATPase beta subunit isoform 3b [Danio rerio] >gi_50370055, AAH76055.1, ATPase, Na+/K+ transporting, beta 3b polypeptide [Danio rerio] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 858 762303 1.2E-02 -0.7 gi_169154330, CAQ15056.1, novel protein similar to vertebrate stromal antigen 1 family [Danio rerio] 80 < 1.0E-180 1116 859 313288 2.0E-02 -0.7 gi_166796353, AAI59234.1, Si:ch211-142e24.2 protein [Danio rerio] 81 < 1.0E-180 1260 860 651416 3.5E-03 -0.7 gi_156383397, XP_001632820.1, predicted protein [Nematostella vectensis] >gi_156219882, 48 5.05E-11 66 EDO40757.1, predicted protein [Nematostella vectensis] 861 741735 < 1.0E-15 -0.7 gi_213511651, NM_001141671.1, Salmo salar homeobox protein HoxA3aa (hoxa3aa), mRNA 97.65 < 1.0E-180 1458 >gi_157816046, EF695307.1, Salmo salar homeobox protein HoxA3aa (HoxA3aa) mRNA, complete cds 862 357882 5.5E-03 -0.8 gi_71834428, NP_001025309.1, apolipoprotein L, 1 [Danio rerio] >gi_33284880, CAE17626.1, novel 90 4.18E-149 470 protein similar to human apolipoprotein 1 (APOL1) [Danio rerio] >gi_133777917, AAI15194.1, Apolipoprotein L, 1 [Danio rerio]

863 543263 2.2E-02 -0.8 gi_410924317, XP_003975628.1, PREDICTED: angiomotin-like 2a-like [Takifugu rubripes] 74 < 1.0E-180 654 864 331917 1.5E-02 -0.8 gi_410923415, XP_003975177.1, PREDICTED: arrestin domain-containing protein 3-like [Takifugu 71 1.89E-29 128 rubripes] 865 20016 8.7E-03 -0.8 gi_410921948, XP_003974445.1, PREDICTED: tyrosine-protein kinase receptor Tie-1-like [Takifugu 87 < 1.0E-180 1751 rubripes] 866 539495 1.3E-03 -0.8 gi_410914455, XP_003970703.1, PREDICTED: LIM domain-binding protein 2-like isoform 2 [Takifugu 95 < 1.0E-180 696 rubripes] 867 619656 2.3E-07 -0.8 gi_410907433, XP_003967196.1, PREDICTED: voltage-dependent calcium channel subunit alpha- 84 < 1.0E-180 1527 2/delta-1-like [Takifugu rubripes] 868 1625 3.9E-12 -0.8 gi_410904951, XP_003965955.1, PREDICTED: inactive serine protease 35-like [Takifugu rubripes] 82 4.24E-170 513 869 499687 1.7E-04 -0.8 gi_40538758, NP_571308.1, GATA-binding protein 2a [Danio rerio] >gi_31544961, AAH53131.1, GATA- 94 6.69E-155 480 binding protein 2a [Danio rerio] 870 115692 5.3E-09 -0.8 gi_348542696, XP_003458820.1, PREDICTED: tripartite motif-containing protein 39-like [Oreochromis 75 1.39E-165 519 niloticus] 871 168735 4.3E-04 -0.8 gi_348542537, XP_003458741.1, PREDICTED: hypothetical protein LOC100695883 [Oreochromis 80 2.67E-19 94 niloticus] 872 59963 7.6E-04 -0.8 gi_348539728, XP_003457341.1, PREDICTED: forkhead box protein F2-like [Oreochromis niloticus] 92 3.63E-138 427 873 144498 < 1.0E-15 -0.8 gi_348536500, XP_003455734.1, PREDICTED: spectrin beta chain, brain 1-like [Oreochromis niloticus] 93 < 1.0E-180 3274 874 716106 1.5E-04 -0.8 gi_348532656, XP_003453822.1, PREDICTED: trafficking kinesin-binding protein 1 [Oreochromis 82 < 1.0E-180 904 niloticus] 875 102908 7.1E-04 -0.8 gi_348530150, XP_003452574.1, PREDICTED: excitatory amino acid transporter 5-like [Oreochromis 91 < 1.0E-180 681 niloticus] 876 52227 2.1E-02 -0.8 gi_348526303, XP_003450659.1, PREDICTED: hepatic leukemia factor-like [Oreochromis niloticus] 68 5.38E-107 355 877 80694 2.9E-10 -0.8 gi_348514696, XP_003444876.1, PREDICTED: semaphorin-3ab-like [Oreochromis niloticus] 91 < 1.0E-180 1049 878 358545 3.1E-05 -0.8 gi_348513781, XP_003444420.1, PREDICTED: zinc-binding protein A33-like [Oreochromis niloticus] 88 < 1.0E-180 917 879 589231 5.5E-02 -0.8 gi_348509459, XP_003442266.1, PREDICTED: LOW QUALITY PROTEIN: calmodulin-regulated 81 7.02E-57 208 spectrin-associated protein 3-like [Oreochromis niloticus] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 880 147546 7.0E-06 -0.8 gi_348505008, XP_003440053.1, PREDICTED: acyl-coenzyme A thioesterase 11-like [Oreochromis 84 < 1.0E-180 980 niloticus] 881 704416 1.3E-14 -0.8 gi_348504396, XP_003439747.1, PREDICTED: UDP-N-acetylhexosamine pyrophosphorylase-like 89 < 1.0E-180 866 [Oreochromis niloticus] 882 76027 3.6E-08 -0.8 gi_348502559, XP_003438835.1, PREDICTED: FERM domain-containing protein 4B [Oreochromis 82 < 1.0E-180 868 niloticus] 883 120987 5.9E-06 -0.8 gi_348501940, XP_003438527.1, PREDICTED: hypothetical protein LOC100705819 [Oreochromis 65 1.22E-16 87 niloticus] 884 278083 1.5E-02 -0.8 gi_326670024, XP_001923882.3, PREDICTED: tumor necrosis factor receptor superfamily member 14 61 4.12E-09 66 [Danio rerio] 885 112860 1.5E-02 -0.8 gi_326665924, XP_002661196.2, PREDICTED: microtubule-associated protein tau [Danio rerio] 75 1.07E-61 224 886 36216 4.0E-05 -0.8 gi_317419235, CBN81272.1, Epidermal growth factor receptor kinase substrate 8-like protein 1 70 3.80E-63 239 [Dicentrarchus labrax] 887 499729 < 1.0E-15 -0.8 gi_317419025, CBN81063.1, GATA-binding factor 2 [Dicentrarchus labrax] 89 1.55E-52 196 888 40000 < 1.0E-15 -0.8 gi_301629132, XP_002943702.1, PREDICTED: hypothetical protein LOC100493523 [Xenopus (Silurana) 79 5.05E-50 143 tropicalis] 889 284068 2.1E-04 -0.8 gi_292617144, XP_695841.4, PREDICTED: G protein-coupled receptor kinase 5-like [Danio rerio] 83 < 1.0E-180 747 890 491309 1.1E-03 -0.8 gi_259155068, NP_001158776.1, sorting nexin-11 [Salmo salar] >gi_223647348, ACN10432.1, Sorting 89 9.99E-158 480 nexin-11 [Salmo salar] 891 332744 3.2E-04 -0.8 gi_259089237, NP_001158656.1, Tumor necrosis factor receptor superfamily member 5 [Oncorhynchus 98 1.04E-103 332 mykiss] >gi_225705634, ACO08663.1, Tumor necrosis factor receptor superfamily member 5 precursor [Oncorhynchus mykiss]

892 89933 9.4E-07 -0.8 gi_226442648, NP_001139917.1, phosphatase 1 regulatory subunit 1C [Salmo salar] >gi_221220010, 84 1.33E-36 146 ACM08666.1, phosphatase 1 regulatory subunit 1C [Salmo salar] 893 160299 2.7E-08 -0.8 gi_225706698, ACO09195.1, Heme-binding protein 2 [Osmerus mordax] 63 5.67E-39 153 894 160300 9.8E-07 -0.8 gi_225706698, ACO09195.1, Heme-binding protein 2 [Osmerus mordax] 70 1.12E-26 101 895 318863 4.5E-09 -0.8 gi_225705582, ACO08637.1, Microsomal glutathione S-transferase 3 [Oncorhynchus mykiss] 98 1.83E-42 162 896 410516 5.3E-09 -0.8 gi_223646616, ACN10066.1, Diamine acetyltransferase 1 [Salmo salar] >gi_223646918, ACN10217.1, 92 1.18E-54 181 Diamine acetyltransferase 1 [Salmo salar] >gi_223672463, ACN12413.1, Diamine acetyltransferase 1 [Salmo salar] >gi_223672781, ACN12572.1, Diamine acetyltransferase 1 [Salmo salar]

897 606960 2.6E-02 -0.8 gi_221221884, ACM09603.1, Glycogenin-1 [Salmo salar] 90 < 1.0E-180 647 898 628799 1.9E-02 -0.8 gi_213515346, NP_001133461.1, acid phosphatase-like protein 2 [Salmo salar] >gi_209154094, 96 < 1.0E-180 933 ACI33279.1, Acid phosphatase-like protein 2 precursor [Salmo salar] 899 160712 3.8E-07 -0.8 gi_213515222, NP_001133173.1, cofilin 2 [Salmo salar] >gi_197632323, ACH70885.1, cofilin 2 [Salmo 100 1.33E-108 333 salar] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 900 545369 6.1E-03 -0.8 gi_213514684, NP_001134305.1, Galectin-3 [Salmo salar] >gi_209732232, ACI66985.1, Galectin-3 81 1.20E-74 257 [Salmo salar] >gi_223647012, ACN10264.1, Galectin-3 [Salmo salar] >gi_223672877, ACN12620.1, Galectin-3 [Salmo salar]

901 26014 7.1E-14 -0.8 gi_213514094, NP_001133534.1, lumican precursor [Salmo salar] >gi_209154384, ACI33424.1, 99 < 1.0E-180 679 Lumican precursor [Salmo salar] 902 11636 8.3E-03 -0.8 gi_213511618, NP_001133951.1, snail homolog Sna [Salmo salar] >gi_209155942, ACI34203.1, snail 98 1.30E-104 338 homolog Sna [Salmo salar] 903 651614 3.5E-04 -0.8 gi_213511554, NP_001134915.1, FAM70B [Salmo salar] >gi_209737164, ACI69451.1, FAM70B [Salmo 85 6.91E-78 252 salar] 904 384953 2.6E-07 -0.8 gi_209730800, ACI66269.1, Glyceraldehyde-3-phosphate dehydrogenase [Salmo salar] 98 < 1.0E-180 593 905 337246 1.3E-02 -0.8 gi_209151020, ACI33056.1, Ornithine decarboxylase 1 [Salmo salar] 99 < 1.0E-180 889 906 667682 8.5E-03 -0.8 gi_189531285, XP_001922973.1, PREDICTED: cdc42 effector protein 2-like [Danio rerio] 59 2.85E-21 101 907 662075 < 1.0E-15 -0.8 gi_185134049, NP_001117742.1, SOX9 alpha2 [Oncorhynchus mykiss] >gi_12003242, AAG43497.1, 95 < 1.0E-180 915 AF209872_1 SOX9 alpha2 [Oncorhynchus mykiss] 908 18352 5.8E-04 -0.8 gi_169640206, DAA06149.1, TPA_exp: claudin 5a [Salmo salar] 98 6.90E-132 396 909 483003 < 1.0E-15 -0.9 gi_71834578, NP_001025391.1, tyrosine-protein kinase Blk [Danio rerio] >gi_55251061, CAH69080.1, 89 < 1.0E-180 798 novel protein tyrosine kinase [Danio rerio] 910 636560 2.8E-04 -0.9 gi_56207587, CAI21294.1, novel protein similar to vertebrate dermatopontin (DPT) [Danio rerio] 82 4.39E-77 254 >gi_213624691, AAI71446.1, Zgc:136613 [Danio rerio] 911 708388 3.2E-06 -0.9 gi_41387132, NP_957104.1, protein phosphatase 1, regulatory (inhibitor) subunit 14Ab [Danio rerio] 88 5.70E-70 241 >gi_37748016, AAH59686.1, Zgc:73377 [Danio rerio] 912 23449 2.6E-09 -0.9 gi_410915690, XP_003971320.1, PREDICTED: rho GTPase-activating protein 31-like [Takifugu rubripes] 88 2.93E-150 515 913 280124 < 1.0E-15 -0.9 gi_410910028, XP_003968492.1, PREDICTED: transmembrane protease serine 13-like [Takifugu 73 < 1.0E-180 585 rubripes] 914 248807 1.8E-12 -0.9 gi_410903912, XP_003965437.1, PREDICTED: proteinase-activated receptor 1-like [Takifugu rubripes] 73 2.43E-103 350 915 758448 3.2E-02 -0.9 gi_363727891, XP_429544.3, PREDICTED: cdc42 effector protein 1 [Gallus gallus] 53 7.73E-43 171 916 387354 1.1E-10 -0.9 gi_348541261, XP_003458105.1, PREDICTED: disco-interacting protein 2 homolog C-like [Oreochromis 98 < 1.0E-180 1030 niloticus] 917 200288 1.3E-05 -0.9 gi_348535210, XP_003455094.1, PREDICTED: protein CYR61-like [Oreochromis niloticus] 76 8.80E-150 460 918 1604 4.6E-04 -0.9 gi_348532101, XP_003453545.1, PREDICTED: inactive serine protease 35-like [Oreochromis niloticus] 87 < 1.0E-180 560 919 114863 6.7E-03 -0.9 gi_348527610, XP_003451312.1, PREDICTED: platelet endothelial aggregation receptor 1-like 83 < 1.0E-180 1368 [Oreochromis niloticus] 920 117675 3.7E-02 -0.9 gi_348523688, XP_003449355.1, PREDICTED: protein yippee-like 2-like [Oreochromis niloticus] 86 2.90E-96 314 921 505774 2.7E-02 -0.9 gi_348521526, XP_003448277.1, PREDICTED: ceramide kinase-like [Oreochromis niloticus] 85 < 1.0E-180 829 922 699381 3.8E-02 -0.9 gi_348516711, XP_003445881.1, PREDICTED: forkhead box protein I1-ema-like [Oreochromis niloticus] 96 2.66E-107 270 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 923 679927 3.3E-05 -0.9 gi_348511729, XP_003443396.1, PREDICTED: fibroblast growth factor-binding protein 2-like 63 4.94E-45 171 [Oreochromis niloticus] 924 476251 1.1E-02 -0.9 gi_348510749, XP_003442907.1, PREDICTED: cyclin-dependent kinase-like 1-like [Oreochromis 94 < 1.0E-180 659 niloticus] 925 321412 < 1.0E-15 -0.9 gi_348509962, XP_003442515.1, PREDICTED: transmembrane protease serine 9-like [Oreochromis 69 1.24E-116 387 niloticus] 926 734905 6.5E-07 -0.9 gi_348508207, XP_003441646.1, PREDICTED: Friend leukemia integration 1 transcription factor isoform 94 < 1.0E-180 827 1 [Oreochromis niloticus] 927 121001 1.7E-03 -0.9 gi_348506214, XP_003440655.1, PREDICTED: solute carrier organic anion transporter family member 64 8.26E-52 203 3A1-like [Oreochromis niloticus] 928 324963 2.1E-06 -0.9 gi_348504234, XP_003439667.1, PREDICTED: hyaluronan and proteoglycan link protein 1-like 91 < 1.0E-180 636 [Oreochromis niloticus] 929 751973 4.9E-05 -0.9 gi_326670654, XP_002663415.2, PREDICTED: collagen alpha-3(VI) chain [Danio rerio] 79 < 1.0E-180 2189 930 515728 4.0E-04 -0.9 gi_296215264, XP_002807289.1, PREDICTED: LOW QUALITY PROTEIN: pleckstrin homology domain- 49 < 1.0E-180 619 containing family G member 3 [Callithrix jacchus] 931 636917 1.6E-04 -0.9 gi_291190874, NP_001167414.1, Glutamate--cysteine ligase regulatory subunit [Salmo salar] 95 1.56E-171 503 >gi_223648596, ACN11056.1, Glutamate--cysteine ligase regulatory subunit [Salmo salar] 932 88693 < 1.0E-15 -0.9 gi_255982818, CAP08959.1, fish virus induced TRIM protein [Oncorhynchus mykiss] 75 1.06E-102 168 933 575393 1.8E-05 -0.9 gi_254028219, NP_001156763.1, G-protein coupled receptor 126 precursor [Danio rerio] 77 < 1.0E-180 1313 >gi_290463239, C6KFA3.1, GP126_DANRE RecName: Full=G-protein coupled receptor 126; Flags: Precursor >gi_246772226, ACS94979.1, Gpr126 [Danio rerio]

934 322179 6.6E-07 -0.9 gi_221219228, ACM08275.1, Claudin-4 [Salmo salar] 93 6.53E-50 168 935 322174 2.0E-06 -0.9 gi_221219228, ACM08275.1, Claudin-4 [Salmo salar] 98 2.21E-61 198 936 246046 5.1E-07 -0.9 gi_221124107, XP_002161911.1, PREDICTED: similar to predicted protein [Hydra magnipapillata] 59 1.87E-43 134 937 359802 < 1.0E-15 -0.9 gi_213515360, NP_001134909.1, Apolipoprotein D precursor [Salmo salar] >gi_209737078, ACI69408.1, 99 4.80E-119 361 Apolipoprotein D precursor [Salmo salar] 938 379354 9.4E-03 -0.9 gi_213515134, NP_001135075.1, Homeobox protein MSX-2 [Salmo salar] >gi_209738484, ACI70111.1, 86 7.74E-140 408 Homeobox protein MSX-2 [Salmo salar] 939 704196 3.6E-03 -0.9 gi_213514874, NP_001135048.1, pirin [Salmo salar] >gi_209738278, ACI70008.1, Pirin [Salmo salar] 98 1.45E-122 381 940 704229 4.9E-02 -0.9 gi_213514874, NP_001135048.1, pirin [Salmo salar] >gi_209738278, ACI70008.1, Pirin [Salmo salar] 100 1.45E-09 63 941 48039 1.1E-05 -0.9 gi_213514228, NP_001133425.1, Retinoblastoma-binding protein 6 [Salmo salar] >gi_209153962, 100 4.09E-65 223 ACI33213.1, Retinoblastoma-binding protein 6 [Salmo salar] 942 102088 1.5E-11 -0.9 gi_213510980, NP_001133947.1, Vimentin [Salmo salar] >gi_209155924, ACI34194.1, Vimentin [Salmo 99 < 1.0E-180 771 salar] 943 628144 9.6E-05 -0.9 gi_209737138, ACI69438.1, HD domain-containing protein 3 [Salmo salar] 94 1.00E-73 243 944 322181 < 1.0E-15 -0.9 gi_209734842, ACI68290.1, Claudin-4 [Salmo salar] >gi_303660255, ADM15987.1, Claudin-4 [Salmo 98 1.36E-98 307 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score salar] 945 304235 3.1E-14 -0.9 gi_198285523, ACH85300.1, hypothetical protein [Salmo salar] 59 1.02E-60 223 946 749264 1.7E-13 -0.9 gi_192447397, NP_001122259.1, procollagen C-endopeptidase enhancer-like precursor [Danio rerio] 69 < 1.0E-180 634 >gi_190338748, AAI63490.1, Similar to procollagen C-endopeptidase enhancer [Danio rerio] >gi_190339011, AAI63473.1, Similar to procollagen C-endopeptidase enhancer [Danio rerio]

947 709683 2.0E-04 -0.9 gi_189525193, XP_001923590.1, PREDICTED: prolargin [Danio rerio] 82 < 1.0E-180 548 948 539662 4.2E-03 -0.9 gi_167555228, NP_001107938.1, uncharacterized protein LOC100000381 precursor [Danio rerio] 89 < 1.0E-180 812 >gi_161612172, AAI55641.1, Zgc:172271 protein [Danio rerio] 949 741749 5.5E-06 -0.9 gi_157816067, ABV82052.1, homeobox protein HoxA3ab [Salmo salar] 99 5.83E-139 449 950 144061 2.8E-05 -0.9 gi_116004559, NP_001070638.1, nephrocystin-1 [Danio rerio] >gi_115313157, AAI24182.1, Im:7162391 76 < 1.0E-180 737 [Danio rerio] >gi_182890872, AAI65641.1, Im:7162391 protein [Danio rerio] 951 111144 < 1.0E-15 -1.0 gi_84993706, BAE76017.1, calcitonin receptor like receptor 3 spliced isoform [Takifugu obscurus] 77 3.23E-84 285 952 329257 1.8E-06 -1.0 gi_68444937, XP_706427.1, PREDICTED: hypothetical protein LOC567961 [Danio rerio] 63 3.18E-27 119 953 748876 4.0E-02 -1.0 gi_47228433, CAG05253.1, unnamed protein product [Tetraodon nigroviridis] 98 4.93E-138 398 954 201248 1.6E-04 -1.0 gi_41386739, NP_958497.1, putative adenosylhomocysteinase 3 [Danio rerio] >gi_37747959, 84 < 1.0E-180 656 AAH59517.1, S-adenosylhomocysteine hydrolase-like 2 [Danio rerio] 955 578127 3.4E-09 -1.0 gi_410929445, XP_003978110.1, PREDICTED: MAP kinase-interacting serine/threonine-protein kinase 90 < 1.0E-180 775 2-like [Takifugu rubripes] 956 257469 1.6E-03 -1.0 gi_410916387, XP_003971668.1, PREDICTED: chaperone activity of bc1 complex-like, mitochondrial- 93 3.66E-16 92 like [Takifugu rubripes] 957 164864 9.1E-03 -1.0 gi_410905659, XP_003966309.1, PREDICTED: sushi, von Willebrand factor type A, EGF and pentraxin 79 < 1.0E-180 522 domain-containing protein 1-like [Takifugu rubripes] 958 366191 2.3E-06 -1.0 gi_410904002, XP_003965482.1, PREDICTED: myocyte-specific enhancer factor 2C-like [Takifugu 76 < 1.0E-180 578 rubripes] 959 57256 5.0E-08 -1.0 gi_410902603, XP_003964783.1, PREDICTED: tripartite motif-containing protein 35-like [Takifugu 82 3.26E-148 459 rubripes] 960 156671 1.3E-04 -1.0 gi_388594055, AFK74524.1, STAC3 alpha [Salmo salar] 98 < 1.0E-180 560 961 398996 1.5E-06 -1.0 gi_363742629, XP_417927.3, PREDICTED: pleckstrin homology-like domain, family B, member 1 [Gallus 69 1.14E-179 581 gallus] 962 656396 2.6E-05 -1.0 gi_348537449, XP_003456207.1, PREDICTED: P2X purinoceptor 5-like [Oreochromis niloticus] 79 < 1.0E-180 625 963 136971 4.7E-02 -1.0 gi_348531840, XP_003453416.1, PREDICTED: angiopoietin-related protein 1-like [Oreochromis 89 < 1.0E-180 821 niloticus] 964 13179 6.6E-03 -1.0 gi_348523313, XP_003449168.1, PREDICTED: E3 ubiquitin-protein ligase Itchy-like [Oreochromis 92 2.71E-52 203 niloticus] 965 159392 1.5E-02 -1.0 gi_348516007, XP_003445531.1, PREDICTED: alcohol dehydrogenase 1-like [Oreochromis niloticus] 91 < 1.0E-180 629 966 80964 1.2E-13 -1.0 gi_348506275, XP_003440685.1, PREDICTED: delta-like protein 4-like [Oreochromis niloticus] 87 < 1.0E-180 1188 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 967 84534 7.5E-14 -1.0 gi_348503570, XP_003439337.1, PREDICTED: otospiralin-like [Oreochromis niloticus] 89 3.38E-42 146 968 263716 3.1E-02 -1.0 gi_345322034, XP_003430525.1, PREDICTED: actin, aortic smooth muscle isoform 2 [Ornithorhynchus 100 2.09E-171 509 anatinus] 969 735879 8.1E-06 -1.0 gi_332227793, XP_003263073.1, PREDICTED: e3 ubiquitin-protein ligase SIAH1-like isoform 2 90 < 1.0E-180 599 [Nomascus leucogenys] 970 70072 6.2E-06 -1.0 gi_327281950, XP_003225708.1, PREDICTED: SPARC-related modular calcium-binding protein 1-like 55 5.91E-86 289 [Anolis carolinensis] 971 158908 1.7E-02 -1.0 gi_326668548, XP_002662360.2, PREDICTED: leucine-rich repeat flightless-interacting protein 2 [Danio 85 3.73E-78 275 rerio] 972 504965 5.2E-02 -1.0 gi_291190658, NP_001167162.1, Calpastatin [Salmo salar] >gi_223648412, ACN10964.1, Calpastatin 96 1.06E-49 191 [Salmo salar] 973 603011 2.0E-05 -1.0 gi_27527053, CAC81754.1, corticotropin-releasing factor receptor type 2 [Oncorhynchus keta] 97 < 1.0E-180 743 974 571378 1.9E-05 -1.0 gi_259089361, NP_001158717.1, Apoptosis-inducing factor-like mitochondrion-associated inducer of 98 < 1.0E-180 585 death [Oncorhynchus mykiss] >gi_225705362, ACO08527.1, Apoptosis-inducing factor-like mitochondrion-associated inducer of death [Oncorhynchus mykiss]

975 724800 1.3E-02 -1.0 gi_224157908, XP_002337912.1, predicted protein [Populus trichocarpa] >gi_222870003, EEF07134.1, 66 1.81E-27 116 predicted protein [Populus trichocarpa] 976 255079 3.6E-07 -1.0 gi_221124107, XP_002161911.1, PREDICTED: similar to predicted protein [Hydra magnipapillata] 69 2.82E-40 92 977 652892 1.2E-02 -1.0 gi_209736324, ACI69031.1, Receptor activity-modifying protein 1 precursor [Salmo salar] 90 9.44E-72 245 >gi_209736696, ACI69217.1, Receptor activity-modifying protein 1 precursor [Salmo salar] 978 160266 2.4E-02 -1.0 gi_209731606, ACI66672.1, Testosterone 17-beta-dehydrogenase 3 [Salmo salar] 99 3.76E-91 299 979 398980 3.3E-14 -1.0 gi_209155138, ACI33801.1, Glutathione reductase, mitochondrial precursor [Salmo salar] 98 4.80E-154 486 980 361105 3.8E-02 -1.0 gi_185133409, NP_001118203.1, growth hormone receptor isoform 2 precursor [Oncorhynchus mykiss] 93 < 1.0E-180 884 >gi_56718398, AAW27914.1, growth hormone receptor type 2b [Oncorhynchus mykiss] 981 225993 1.2E-03 -1.0 gi_148725560, CAN88064.1, novel protein similar to vertebrate acyl-CoA thioesterase 11 (ACOT11, 83 5.50E-15 84 zgc:113011) [Danio rerio] >gi_169145180, CAQ14225.1, novel protein similar to vertebrate acyl-CoA thioesterase 11 (ACOT11, zgc:113011) [Danio rerio]

982 208576 2.4E-04 -1.0 gi_146147385, ABQ01988.1, reverse transcriptase-like protein [Salmo salar] >gi_148362149, 69 1.82E-37 140 ABQ59672.1, reverse transcriptase-like protein [Salmo salar] >gi_260182170, ACX35599.1, reverse transcriptase-like protein [Salmo salar]

983 122365 4.5E-06 -1.0 gi_118150440, NP_001071199.1, uncharacterized protein LOC777623 [Danio rerio] >gi_116487638, 74 8.62E-95 307 AAI25955.1, Zgc:154093 [Danio rerio] 984 329323 2.2E-02 -1.1 gi_83722645, YP_443922.1, polyprotein [Atlantic salmon swim bladder sarcoma virus] >gi_76786463, 84 2.83E-41 170 ABA54982.1, polyprotein [Atlantic salmon swim bladder sarcoma virus] 985 263698 3.2E-02 -1.1 gi_67968880, BAE00797.1, unnamed protein product [Macaca fascicularis] 99 2.38E-172 505 986 549383 6.0E-03 -1.1 gi_51871619, NP_001004109.1, quattro [Danio rerio] >gi_50086979, AAT70410.1, quattro [Danio rerio] 85 1.62E-55 219 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 987 708380 6.7E-12 -1.1 gi_41387132, NP_957104.1, protein phosphatase 1, regulatory (inhibitor) subunit 14Ab [Danio rerio] 89 2.01E-71 245 >gi_37748016, AAH59686.1, Zgc:73377 [Danio rerio] 988 715827 6.5E-03 -1.1 gi_410929235, XP_003978005.1, PREDICTED: protein FAM69C-like [Takifugu rubripes] 78 < 1.0E-180 577 989 48639 4.3E-02 -1.1 gi_410920583, XP_003973763.1, PREDICTED: DEP domain-containing mTOR-interacting protein-like 84 3.29E-140 429 [Takifugu rubripes] 990 241275 2.4E-07 -1.1 gi_410920085, XP_003973514.1, PREDICTED: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 92 < 1.0E-180 497 4-like isoform 1 [Takifugu rubripes] 991 304436 4.6E-10 -1.1 gi_410912774, XP_003969864.1, PREDICTED: membrane progestin receptor gamma-B-like [Takifugu 79 3.89E-57 209 rubripes] 992 333008 6.1E-06 -1.1 gi_410896434, XP_003961704.1, PREDICTED: LIM domain only protein 7-like [Takifugu rubripes] 62 8.78E-115 323 993 73015 8.1E-14 -1.1 gi_348542553, XP_003458749.1, PREDICTED: PHD finger protein 23B-like [Oreochromis niloticus] 80 2.11E-110 362 994 297216 4.7E-02 -1.1 gi_348520985, XP_003448007.1, PREDICTED: mucolipin-1-like [Oreochromis niloticus] 93 < 1.0E-180 575 995 545383 2.7E-06 -1.1 gi_348520919, XP_003447974.1, PREDICTED: kelch-like ECH-associated protein 1-like [Oreochromis 96 < 1.0E-180 1034 niloticus] 996 80937 5.5E-09 -1.1 gi_348515605, XP_003445330.1, PREDICTED: palmitoyltransferase ZDHHC9-like [Oreochromis 89 < 1.0E-180 626 niloticus] 997 158137 2.6E-04 -1.1 gi_348511221, XP_003443143.1, PREDICTED: myosin-10-like [Oreochromis niloticus] 94 < 1.0E-180 1440 998 62716 2.1E-03 -1.1 gi_348511023, XP_003443044.1, PREDICTED: syntaxin-11-like [Oreochromis niloticus] 64 2.43E-75 258 999 234706 1.5E-02 -1.1 gi_348507599, XP_003441343.1, PREDICTED: FYVE, RhoGEF and PH domain-containing protein 5 88 < 1.0E-180 609 [Oreochromis niloticus] 1000 34358 < 1.0E-15 -1.1 gi_348506156, XP_003440626.1, PREDICTED: UPF0577 protein KIAA1324-like [Oreochromis niloticus] 85 < 1.0E-180 1189 1001 225511 8.7E-05 -1.1 gi_348505008, XP_003440053.1, PREDICTED: acyl-coenzyme A thioesterase 11-like [Oreochromis 95 1.48E-27 91 niloticus] 1002 222374 1.1E-02 -1.1 gi_348502900, XP_003439005.1, PREDICTED: protein phosphatase 1 regulatory subunit 12B-like 88 8.32E-73 247 [Oreochromis niloticus] 1003 43327 4.5E-10 -1.1 gi_348501238, XP_003438177.1, PREDICTED: 3-ketodihydrosphingosine reductase-like [Oreochromis 94 1.64E-167 429 niloticus] 1004 693592 4.6E-02 -1.1 gi_327265065, XP_003217329.1, PREDICTED: LOW QUALITY PROTEIN: epsin-3-like [Anolis 59 9.99E-134 422 carolinensis] 1005 48213 1.7E-04 -1.1 gi_326673117, XP_002664258.2, PREDICTED: lysyl oxidase homolog 4 [Danio rerio] 89 < 1.0E-180 1315 1006 91022 5.5E-05 -1.1 gi_326671914, XP_003199551.1, PREDICTED: hypothetical protein LOC321155 [Danio rerio] 48 1.94E-51 206 1007 249791 2.3E-06 -1.1 gi_326668677, XP_001333128.4, PREDICTED: hypothetical protein LOC794764 [Danio rerio] 55 6.32E-33 141 1008 29628 4.1E-08 -1.1 gi_312102827, XP_003150007.1, hypothetical protein LOAG_14462 [Loa loa] >gi_307754829, 51 1.29E-04 47 EFO14063.1, hypothetical protein LOAG_14462, partial [Loa loa] 1009 355838 1.0E-04 -1.1 gi_29841219, AAP06232.1, similar to GenBank Accession Number AB073376 non-LTR 97 8.53E-15 84 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score retrotransposable element partially supported by GENSCAN in Oryzias latipes [Schistosoma japonicum]

1010 224009 2.7E-06 -1.1 gi_229367954, ACQ58957.1, Annexin A2 [Anoplopoma fimbria] 72 1.65E-97 319 1011 638532 3.4E-13 -1.1 gi_226442648, NP_001139917.1, phosphatase 1 regulatory subunit 1C [Salmo salar] >gi_221220010, 94 5.15E-95 296 ACM08666.1, phosphatase 1 regulatory subunit 1C [Salmo salar] 1012 384908 1.5E-12 -1.1 gi_209730800, ACI66269.1, Glyceraldehyde-3-phosphate dehydrogenase [Salmo salar] 99 4.58E-124 387 1013 413139 3.2E-04 -1.1 gi_181330207, NP_001116711.1, uncharacterized protein LOC557488 precursor [Danio rerio] 54 3.70E-17 89 >gi_169154280, CAQ13401.1, novel protein [Danio rerio] 1014 383228 3.0E-11 -1.2 gi_410903516, XP_003965239.1, PREDICTED: G-protein-signaling modulator 1-like [Takifugu rubripes] 92 < 1.0E-180 1120 1015 297185 3.7E-13 -1.2 gi_410899092, XP_003963031.1, PREDICTED: RING finger protein 223-like [Takifugu rubripes] 68 1.84E-65 225 1016 531090 1.3E-02 -1.2 gi_371902292, CAZ61326.1, CD34b2 molecule, partial [Oncorhynchus mykiss] 95 6.50E-107 335 1017 540991 4.6E-03 -1.2 gi_348544811, XP_003459874.1, PREDICTED: seizure protein 6 homolog [Oreochromis niloticus] 90 < 1.0E-180 1002 1018 637221 1.0E-09 -1.2 gi_348523021, XP_003449022.1, PREDICTED: latent-transforming growth factor beta-binding protein 2- 75 < 1.0E-180 1016 like [Oreochromis niloticus] 1019 23910 2.1E-11 -1.2 gi_348503073, XP_003439091.1, PREDICTED: calcium/calmodulin-dependent protein kinase type 1G- 87 < 1.0E-180 665 like [Oreochromis niloticus] 1020 162468 1.2E-07 -1.2 gi_326668677, XP_001333128.4, PREDICTED: hypothetical protein LOC794764 [Danio rerio] 59 5.29E-40 157 1021 40055 4.9E-02 -1.2 gi_317419516, CBN81553.1, Carbohydrate sulfotransferase 15 [Dicentrarchus labrax] 88 < 1.0E-180 884 1022 132294 4.6E-02 -1.2 gi_291190900, NP_001167070.1, peroxisomal Lon protease homolog 2 [Salmo salar] >gi_223647948, 97 < 1.0E-180 1030 ACN10732.1, Peroxisomal Lon protease homolog 2 [Salmo salar] 1023 216386 4.0E-02 -1.2 gi_225715552, ACO13622.1, 40S ribosomal protein S27 [Esox lucius] 97 4.58E-50 176 1024 742247 1.5E-06 -1.2 gi_225715518, ACO13605.1, Four and a half LIM domains protein 1 [Esox lucius] 97 < 1.0E-180 581 1025 176380 4.5E-04 -1.2 gi_213511210, NP_001134117.1, PDZ and LIM domain protein 3 [Salmo salar] >gi_209730834, 100 7.82E-144 424 ACI66286.1, PDZ and LIM domain protein 3 [Salmo salar] 1026 284791 1.2E-05 -1.2 gi_209735888, ACI68813.1, UPF0527 membrane protein [Salmo salar] 66 2.18E-05 50 1027 398941 3.2E-02 -1.2 gi_209155138, ACI33801.1, Glutathione reductase, mitochondrial precursor [Salmo salar] 93 8.70E-13 80 1028 559352 2.2E-09 -1.2 gi_189534157, XP_001920911.1, PREDICTED: apoptosis-inducing factor 3-like [Danio rerio] 78 < 1.0E-180 587 1029 144666 3.4E-06 -1.2 gi_160333855, NP_001103956.1, filamin A-interacting protein 1-like [Danio rerio] >gi_160385738, 76 < 1.0E-180 922 A3KNA5.1, FIL1L_DANRE RecName: Full=Filamin A-interacting protein 1-like >gi_126631251, AAI33735.1, Zgc:158292 protein [Danio rerio]

1030 25329 < 1.0E-15 -1.2 gi_148231115, NP_001085253.1, protein tyrosine phosphatase type IVA, member 3 [Xenopus laevis] 96 5.05E-109 335 >gi_48290364, BAD22567.1, xPRL-3 [Xenopus laevis] >gi_213623524, AAI69861.1, XPRL-3 [Xenopus laevis] >gi_213626000, AAI69716.1, XPRL-3 [Xenopus laevis]

1031 606464 2.9E-02 -1.2 gi_113682448, NP_001038893.1, ras-related protein Rab-33A [Danio rerio] >gi_112418990, AAI22390.1, 86 1.79E-126 380 Zgc:153710 [Danio rerio] >gi_182889420, AAI65073.1, Zgc:153710 protein [Danio rerio] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 1032 405916 < 1.0E-15 -1.3 gi_47226981, CAG05873.1, unnamed protein product [Tetraodon nigroviridis] 81 8.87E-88 314 1033 552046 2.8E-03 -1.3 gi_410900304, XP_003963636.1, PREDICTED: membrane-associated guanylate kinase, WW and PDZ 68 < 1.0E-180 823 domain-containing protein 3-like [Takifugu rubripes] 1034 743821 1.3E-02 -1.3 gi_348521822, XP_003448425.1, PREDICTED: alpha-1,3-galactosyltransferase 2-like [Oreochromis 86 3.59E-168 490 niloticus] 1035 620155 3.3E-05 -1.3 gi_348502957, XP_003439033.1, PREDICTED: synapsin-2-like isoform 1 [Oreochromis niloticus] 85 3.71E-170 522 1036 227852 5.2E-02 -1.3 gi_326676679, XP_003200646.1, PREDICTED: retrotransposable element Tf2 155 kDa protein type 1- 76 1.19E-153 300 like [Danio rerio] 1037 90881 5.4E-02 -1.3 gi_296189079, XP_002742638.1, PREDICTED: sciellin [Callithrix jacchus] 62 7.15E-18 96 1038 60621 1.3E-06 -1.3 gi_221221674, ACM09498.1, Whey acidic protein precursor [Salmo salar] 98 6.51E-118 345 1039 187155 1.1E-02 -1.3 gi_18859567, NP_571012.1, protein Wnt-5b precursor [Danio rerio] >gi_2501662, Q92050.1, 94 < 1.0E-180 705 WNT5B_DANRE RecName: Full=Protein Wnt-5b; Flags: Precursor >gi_1256778, AAA96519.1, Wnt5 [Danio rerio] >gi_148726430, CAN88522.1, wingless-type MMTV integration site family, member 5b [Danio rerio] >gi_190337200, AAI62984.1, Wingless-type MMTV integration site family, member 5b [Danio rerio] >gi_190339644, AAI62981.1, Wingless-type MMTV integration site family, member 5b [Danio rerio] 1040 707835 3.2E-07 -1.3 gi_185135614, NP_001117782.1, toxin-1 precursor [Oncorhynchus mykiss] >gi_20385167, AAM21198.1, 98 5.91E-41 144 AF363273_1 toxin-1 [Oncorhynchus mykiss] 1041 263920 1.3E-06 -1.3 gi_104532042, ABF72904.1, muscle-specific actin-like [Belgica antarctica] 98 1.15E-81 254 1042 642635 2.4E-02 -1.4 gi_62955239, NP_001017635.1, E3 ubiquitin-protein ligase parkin [Danio rerio] >gi_62531267, 85 < 1.0E-180 742 AAH93281.1, Parkinson disease (autosomal recessive, juvenile) 2, parkin [Danio rerio] >gi_182890500, AAI64537.1, Park2 protein [Danio rerio]

1043 175146 < 1.0E-15 -1.4 gi_410924878, XP_003975908.1, PREDICTED: c-X-C chemokine receptor type 4-like [Takifugu rubripes] 86 1.02E-175 509 1044 639593 6.4E-03 -1.4 gi_410900376, XP_003963672.1, PREDICTED: calcium homeostasis modulator protein 3-like [Takifugu 91 5.07E-178 515 rubripes] 1045 159116 1.4E-04 -1.4 gi_348513587, XP_003444323.1, PREDICTED: LOW QUALITY PROTEIN: solute carrier organic anion 83 < 1.0E-180 723 transporter family member 2A1-like [Oreochromis niloticus] 1046 605786 3.8E-04 -1.4 gi_348513175, XP_003444118.1, PREDICTED: glutamate [NMDA] receptor-associated protein 1-like 96 8.87E-112 363 [Oreochromis niloticus] 1047 658952 3.4E-02 -1.4 gi_348511346, XP_003443205.1, PREDICTED: hypothetical protein LOC100707003 [Oreochromis 73 2.54E-65 168 niloticus] 1048 612604 2.7E-04 -1.4 gi_317419175, CBN81212.1, Heat-stable enterotoxin receptor [Dicentrarchus labrax] 84 < 1.0E-180 1376 1049 356981 2.0E-03 -1.4 gi_226443370, NP_001140147.1, CDP-diacylglycerol--serine O-phosphatidyltransferase [Salmo salar] 100 3.15E-04 50 >gi_221222276, ACM09799.1, CDP-diacylglycerol--serine O-phosphatidyltransferase [Salmo salar] 1050 702976 3.8E-03 -1.4 gi_223649112, ACN11314.1, Pyruvate dehydrogenase kinase isozyme 2, mitochondrial precursor [Salmo 100 4.67E-50 177 salar] 1051 702948 1.4E-02 -1.4 gi_223649112, ACN11314.1, Pyruvate dehydrogenase kinase isozyme 2, mitochondrial precursor [Salmo 100 4.94E-24 113 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score salar] 1052 235619 1.2E-03 -1.4 gi_157422949, AAI53507.1, Zgc:174162 protein [Danio rerio] 48 2.51E-07 61 1053 589893 2.7E-02 -1.5 gi_76163164, AAX30962.2, SJCHGC09651 protein [Schistosoma japonicum] 74 9.92E-05 45 1054 183750 1.8E-05 -1.5 gi_66472590, NP_001018417.1, rab-3A-interacting protein [Danio rerio] >gi_63100634, AAH95215.1, 88 < 1.0E-180 582 Zgc:110270 [Danio rerio] 1055 323107 4.4E-13 -1.5 gi_354471245, XP_003497853.1, PREDICTED: prominin-2-like isoform 2 [Cricetulus griseus] 48 1.63E-55 218 1056 135672 1.1E-14 -1.5 gi_348542332, XP_003458639.1, PREDICTED: metastasis suppressor protein 1-like [Oreochromis 77 < 1.0E-180 907 niloticus] 1057 611298 < 1.0E-15 -1.5 gi_348523764, XP_003449393.1, PREDICTED: hypothetical protein LOC100690300 [Oreochromis 65 7.79E-14 77 niloticus] 1058 95831 2.5E-02 -1.5 gi_348523407, XP_003449215.1, PREDICTED: hepatocyte growth factor receptor-like [Oreochromis 95 < 1.0E-180 622 niloticus] 1059 541582 4.9E-05 -1.5 gi_348511147, XP_003443106.1, PREDICTED: nuclear receptor subfamily 1 group D member 2-like 87 4.76E-67 174 [Oreochromis niloticus] 1060 250551 1.9E-04 -1.5 gi_301607131, XP_002933173.1, PREDICTED: hypothetical protein LOC100488659 [Xenopus (Silurana) 78 1.40E-78 258 tropicalis] 1061 554130 8.3E-07 -1.5 gi_225707700, ACO09696.1, Claudin-8 [Osmerus mordax] 85 5.18E-98 312 1062 220689 2.6E-08 -1.5 gi_225705084, ACO08388.1, Thioredoxin [Oncorhynchus mykiss] 100 9.16E-21 94 1063 706634 4.9E-05 -1.5 gi_190358626, NP_001121817.1, uncharacterized protein LOC566600 [Danio rerio] >gi_122890777, 75 1.39E-38 130 CAM14226.1, novel protein [Danio rerio] 1064 64904 1.5E-03 -1.5 gi_185135940, NP_001117933.1, Na/K ATPase alpha subunit isoform 1a [Oncorhynchus mykiss] 97 < 1.0E-180 1053 >gi_34812027, AAQ82790.1, Na/K ATPase alpha subunit isoform 1a [Oncorhynchus mykiss] 1065 317335 1.1E-05 -1.5 gi_185135740, NP_001117965.1, caspace-like protein [Oncorhynchus mykiss] >gi_51038275, 72 1.85E-16 89 AAT94059.1, caspace-like protein [Oncorhynchus mykiss] 1066 93011 < 1.0E-15 -1.5 gi_163256399, BAC10574.2, nrf2-associated protein keap1a [Danio rerio] 88 < 1.0E-180 470 1067 220717 1.2E-14 -1.6 gi_410930458, XP_003978615.1, PREDICTED: prostaglandin reductase 1-like [Takifugu rubripes] 85 2.73E-142 434 1068 63487 2.8E-05 -1.6 gi_410913579, XP_003970266.1, PREDICTED: transmembrane protein 179-like [Takifugu rubripes] 73 4.47E-51 146 1069 681400 4.7E-02 -1.6 gi_410912888, XP_003969921.1, PREDICTED: immunoglobulin-like domain-containing receptor 1-like 90 4.18E-41 159 [Takifugu rubripes] 1070 64932 1.7E-03 -1.6 gi_410906317, XP_003966638.1, PREDICTED: sodium/potassium-transporting ATPase subunit alpha-1- 84 < 1.0E-180 384 like [Takifugu rubripes] 1071 525468 1.0E-14 -1.6 gi_348541245, XP_003458097.1, PREDICTED: vacuole membrane protein 1-like [Oreochromis niloticus] 83 < 1.0E-180 594 1072 498313 1.4E-03 -1.6 gi_225716670, ACO14181.1, 3-oxoacyl-acyl-carrier-protein reductase [Esox lucius] 97 5.99E-149 469 1073 412786 < 1.0E-15 -1.6 gi_213514162, NP_001134788.1, Arginase-1 [Salmo salar] >gi_209736026, ACI68882.1, Arginase-1 99 < 1.0E-180 674 [Salmo salar] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 1074 29599 1.6E-06 -1.6 gi_209737262, ACI69500.1, Brain protein 44 [Salmo salar] 91 1.62E-69 226 1075 628176 4.5E-06 -1.6 gi_209737138, ACI69438.1, HD domain-containing protein 3 [Salmo salar] 100 1.73E-23 99 1076 163717 < 1.0E-15 -1.6 gi_189534278, XP_001339010.2, PREDICTED: rho GTPase-activating protein 24 [Danio rerio] 80 < 1.0E-180 1029 1077 331823 1.3E-14 -1.7 gi_47224919, CAG06489.1, unnamed protein product [Tetraodon nigroviridis] 76 4.13E-35 136 1078 69055 7.1E-04 -1.7 gi_410911932, XP_003969444.1, PREDICTED: HEPACAM family member 2-like [Takifugu rubripes] 76 3.73E-180 543 1079 379154 9.5E-04 -1.7 gi_357575246, AET85182.1, TC1-like transposase [Cyprinus carpio] 65 4.12E-55 138 1080 465947 1.3E-05 -1.7 gi_348542762, XP_003458853.1, PREDICTED: hemicentin-2-like [Oreochromis niloticus] 61 6.53E-93 270 1081 699973 4.5E-06 -1.7 gi_348519214, XP_003447126.1, PREDICTED: cat eye syndrome critical region protein 5 homolog 67 < 1.0E-180 575 [Oreochromis niloticus] 1082 33097 4.3E-03 -1.7 gi_326678711, XP_001343650.4, PREDICTED: hypothetical protein LOC100004316 [Danio rerio] 73 < 1.0E-180 874 1083 340299 3.1E-03 -1.7 gi_323649958, ADX97065.1, amine sulfotransferase [Perca flavescens] 79 2.20E-133 417 1084 558739 1.7E-03 -1.7 gi_301614772, XP_002936863.1, PREDICTED: hypothetical protein LOC100493551 [Xenopus (Silurana) 69 1.49E-55 215 tropicalis] 1085 202261 8.1E-04 -1.7 gi_301612068, XP_002935557.1, PREDICTED: extracellular matrix protein 2-like [Xenopus (Silurana) 74 7.47E-96 242 tropicalis] 1086 283781 < 1.0E-15 -1.7 gi_291227761, XP_002733851.1, PREDICTED: RETRotransposon-like family member (retr-1)-like 67 2.99E-83 112 [Saccoglossus kowalevskii] 1087 465674 2.3E-03 -1.7 gi_169154038, CAQ13811.1, novel protein similar to vertebrate titin (TTN) [Danio rerio] 48 4.35E-33 116 1088 169592 3.9E-02 -1.7 gi_125846594, XP_690746.2, PREDICTED: androgen-induced gene 1 protein [Danio rerio] 92 8.22E-99 304 1089 31995 6.5E-09 -1.8 gi_47219243, CAG11705.1, unnamed protein product [Tetraodon nigroviridis] 55 1.88E-163 570 1090 49978 2.6E-05 -1.8 gi_410901741, XP_003964354.1, PREDICTED: tryptase-2-like [Takifugu rubripes] 69 2.59E-57 207 1091 754649 3.9E-02 -1.8 gi_397471308, XP_003807238.1, PREDICTED: collagen alpha-1(XXVI) chain [Pan paniscus] 67 1.07E-37 152 1092 699994 1.0E-05 -1.8 gi_355623185, ZP_09047056.1, hypothetical protein HMPREF1020_01135 [Clostridium sp. 7_3_54FAA] 50 1.77E-04 46 >gi_354822513, EHF06871.1, hypothetical protein HMPREF1020_01135 [Clostridium sp. 7_3_54FAA] 1093 145947 2.7E-03 -1.8 gi_348536556, XP_003455762.1, PREDICTED: coiled-coil domain-containing protein 3-like [Oreochromis 85 1.34E-120 367 niloticus] 1094 710221 1.9E-08 -1.8 gi_348532648, XP_003453818.1, PREDICTED: bifunctional protein NCOAT-like [Oreochromis niloticus] 86 < 1.0E-180 839 1095 743469 < 1.0E-15 -1.8 gi_348530238, XP_003452618.1, PREDICTED: zinc transporter ZIP3-like [Oreochromis niloticus] 87 4.75E-103 337 1096 149857 2.8E-04 -1.8 gi_348517477, XP_003446260.1, PREDICTED: hypothetical protein LOC100697950 [Oreochromis 61 2.32E-04 53 niloticus] 1097 613292 3.1E-04 -1.8 gi_326680337, XP_002666925.2, PREDICTED: receptor-type tyrosine-protein phosphatase zeta [Danio 84 < 1.0E-180 880 rerio] 1098 663358 2.6E-04 -1.8 gi_32394793, AAN15747.1, reverse transcriptase-like protein [Paralichthys olivaceus] 54 9.28E-08 59 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 1099 291697 1.6E-04 -1.8 gi_302854046, XP_002958534.1, hypothetical protein VOLCADRAFT_69561 [Volvox carteri f. 46 1.31E-04 50 nagariensis] >gi_300256109, EFJ40383.1, hypothetical protein VOLCADRAFT_69561 [Volvox carteri f. nagariensis] 1100 513930 9.3E-03 -1.8 gi_260785652, XP_002587874.1, hypothetical protein BRAFLDRAFT_87261 [Branchiostoma floridae] 38 4.41E-08 62 >gi_229273029, EEN43885.1, hypothetical protein BRAFLDRAFT_87261 [Branchiostoma floridae] 1101 261074 3.9E-12 -1.8 gi_225707704, ACO09698.1, Sulfotransferase 6B1 [Osmerus mordax] 83 4.17E-56 200 1102 655754 1.1E-02 -1.8 gi_213512345, NP_001133896.1, peptidyl-prolyl cis-trans isomerase FKBP4 [Salmo salar] 98 1.96E-125 392 >gi_209155736, ACI34100.1, FK506-binding protein 4 [Salmo salar] 1103 230283 7.8E-04 -1.8 gi_156380590, XP_001631851.1, predicted protein [Nematostella vectensis] >gi_156218898, 50 1.01E-08 65 EDO39788.1, predicted protein [Nematostella vectensis] 1104 655361 1.2E-12 -1.9 gi_50344860, NP_001002102.1, purine nucleoside phosphorylase 4a [Danio rerio] >gi_47939464, 90 9.77E-179 518 AAH71515.1, Zgc:86889 [Danio rerio] >gi_157888633, CAE30433.2, novel protein similar to mouse purine-nucleoside phosphorylase (PNP, zgc:86889) [Danio rerio]

1105 267764 6.4E-08 -1.9 gi_348535644, XP_003455309.1, PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like 84 < 1.0E-180 1027 [Oreochromis niloticus] 1106 261139 2.0E-04 -1.9 gi_301629132, XP_002943702.1, PREDICTED: hypothetical protein LOC100493523 [Xenopus (Silurana) 75 1.06E-14 79 tropicalis] 1107 201144 2.0E-08 -2.0 gi_41152388, NP_956302.1, pancreatic progenitor cell differentiation and proliferation factor B [Danio 93 1.37E-33 129 rerio] >gi_82186897, Q6PBI2.1, PDPFB_DANRE RecName: Full=Pancreatic progenitor cell differentiation and proliferation factor B; AltName: Full=Exocrine differentiation and proliferation factor B >gi_37590414, AAH59699.1, C20orf149 homolog (H. sapiens), b [Danio rerio]

1108 264776 < 1.0E-15 -2.0 gi_404250106, AFR53956.1, transposase [Cloning vector pT2/Gene-Trap] 93 2.22E-58 203 1109 465982 < 1.0E-15 -2.0 gi_348542760, XP_003458852.1, PREDICTED: hemicentin-1-like [Oreochromis niloticus] 57 3.03E-90 324 1110 181123 1.3E-06 -2.0 gi_326676095, XP_003200505.1, PREDICTED: perilipin-1 [Danio rerio] 68 3.97E-71 245 1111 261155 < 1.0E-15 -2.0 gi_221220976, ACM09149.1, Sulfotransferase 6B1 [Salmo salar] 88 8.06E-14 77 1112 132211 2.2E-02 -2.0 gi_209736498, ACI69118.1, Transmembrane and TPR repeat-containing protein 4 [Salmo salar] 86 5.05E-17 89 1113 7912 1.9E-02 -2.0 gi_157841250, NP_001103183.1, G-protein coupled receptor family C group 5 member C precursor 79 5.04E-155 468 [Danio rerio] >gi_156229870, AAI51954.1, Gprc5c protein [Danio rerio] 1114 193817 2.5E-05 -2.1 gi_348542981, XP_003458962.1, PREDICTED: vomeronasal type-2 receptor 1-like [Oreochromis 68 1.31E-13 60 niloticus] 1115 680139 5.4E-02 -2.1 gi_348517702, XP_003446372.1, PREDICTED: clusterin-like [Oreochromis niloticus] 69 2.42E-118 371 1116 323124 < 1.0E-15 -2.1 gi_344248867, EGW04971.1, Prominin-2 [Cricetulus griseus] 50 9.39E-43 179 1117 560661 < 1.0E-15 -2.1 gi_326676287, XP_001921093.3, PREDICTED: estrogen-related receptor gamma [Danio rerio] 78 < 1.0E-180 589 1118 504878 1.6E-02 -2.2 gi_348533397, XP_003454192.1, PREDICTED: kremen protein 1-like [Oreochromis niloticus] 93 1.79E-29 130 1119 358876 3.9E-05 -2.2 gi_348532193, XP_003453591.1, PREDICTED: regulator of G-protein signaling 4-like [Oreochromis 79 8.75E-71 245 niloticus] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 1120 550800 5.3E-02 -2.2 gi_301629132, XP_002943702.1, PREDICTED: hypothetical protein LOC100493523 [Xenopus (Silurana) 77 2.35E-17 97 tropicalis] 1121 261134 < 1.0E-15 -2.2 gi_224613232, ACN60195.1, Retrotransposable element Tf2 155 kDa protein type 3 [Salmo salar] 70 1.56E-53 207 1122 110805 1.3E-05 -2.2 gi_185133660, NP_001117732.1, apolipoprotein CII precursor [Oncorhynchus mykiss] >gi_9992871, 94 1.08E-51 176 AAG11410.1, AF140783_1 apolipoprotein CII [Oncorhynchus mykiss] 1123 412789 3.6E-14 -2.3 gi_52138509, DAA01450.1, TPA_exp: arginase type I [Oncorhynchus mykiss] 98 8.16E-114 344 1124 643863 < 1.0E-15 -2.3 gi_410912248, XP_003969602.1, PREDICTED: iroquois-class homeodomain protein irx-3-like [Takifugu 91 < 1.0E-180 656 rubripes] 1125 290063 4.1E-02 -2.3 gi_410898395, XP_003962683.1, PREDICTED: autophagy-related protein 2 homolog B-like [Takifugu 80 4.42E-82 232 rubripes] 1126 563374 8.0E-05 -2.3 gi_348525450, XP_003450235.1, PREDICTED: hypothetical protein LOC100694880 [Oreochromis 68 2.25E-52 198 niloticus] 1127 602696 2.5E-02 -2.3 gi_326666222, XP_003198219.1, PREDICTED: retrotransposable element Tf2 155 kDa protein type 1- 73 3.25E-25 80 like [Danio rerio] 1128 576697 7.1E-03 -2.3 gi_118596560, BAF37936.1, Tc1-like transporase [Oncorhynchus mykiss] 66 3.00E-13 60 1129 118305 1.6E-04 -2.4 gi_348522173, XP_003448600.1, PREDICTED: low-density lipoprotein receptor-related protein 1B-like 77 < 1.0E-180 651 [Oreochromis niloticus] 1130 140751 1.4E-02 -2.4 gi_348509187, XP_003442133.1, PREDICTED: hsp90 co-chaperone Cdc37-like [Oreochromis niloticus] 88 1.36E-165 503 1131 239306 1.7E-02 -2.4 gi_116004497, NP_001070610.1, uncharacterized protein LOC556036 [Danio rerio] >gi_115313861, 89 1.64E-10 74 AAI24525.1, Zgc:154055 [Danio rerio] >gi_182892064, AAI65766.1, Zgc:154055 protein [Danio rerio] 1132 169376 2.7E-02 -2.5 gi_47230458, CAF99651.1, unnamed protein product [Tetraodon nigroviridis] 68 9.53E-38 151 1133 706930 1.6E-02 -2.5 gi_397524284, XP_003832130.1, PREDICTED: collagen alpha-1(IV) chain [Pan paniscus] 66 < 1.0E-180 1153 >gi_410298984, JAA28092.1, collagen, type IV, alpha 1 [Pan troglodytes] >gi_410339999, JAA38946.1, collagen, type IV, alpha 1 [Pan troglodytes]

1134 656920 1.7E-08 -2.5 gi_317419955, CBN81991.1, Villin-1 [Dicentrarchus labrax] 90 1.25E-23 111 1135 342523 2.1E-02 -2.5 gi_213513191, NP_001134157.1, Carbonic anhydrase 12 precursor [Salmo salar] >gi_209731112, 97 2.79E-11 69 ACI66425.1, Carbonic anhydrase 12 precursor [Salmo salar] >gi_303657463, ADM15876.1, Carbonic anhydrase 12 precursor [Salmo salar]

1136 283757 7.9E-05 -2.5 gi_213512890, NP_001134781.1, vesicle-associated membrane protein 5-like [Salmo salar] 96 1.39E-09 60 >gi_209735950, ACI68844.1, Vesicle-associated membrane protein 5 [Salmo salar] 1137 219852 5.0E-02 -2.6 gi_76162473, AAX30323.2, SJCHGC03012 protein [Schistosoma japonicum] 76 6.60E-14 55 1138 206523 2.9E-02 -2.6 gi_56755641, AAW25999.1, SJCHGC07569 protein [Schistosoma japonicum] 59 8.71E-19 92 1139 591530 < 1.0E-15 -2.6 gi_410903321, XP_003965142.1, PREDICTED: phosphatidylinositol 4-phosphate 5-kinase-like protein 1- 68 1.17E-62 227 like [Takifugu rubripes] 1140 379046 4.0E-02 -2.6 gi_410896043, XP_003961509.1, PREDICTED: vesicular glutamate transporter 1-like [Takifugu rubripes] 93 < 1.0E-180 1028 1141 660425 1.1E-04 -2.6 gi_317419955, CBN81991.1, Villin-1 [Dicentrarchus labrax] 86 < 1.0E-180 504 AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 1142 626087 1.1E-07 -2.8 gi_60701364, AAX31108.1, SJCHGC09823 protein [Schistosoma japonicum] 87 5.48E-24 86 1143 595101 8.2E-06 -2.8 gi_185135617, NP_001117690.1, 11-beta-hydroxysteroid dehydrogenase [Oncorhynchus mykiss] 94 1.16E-98 309 >gi_30844232, BAC76709.1, 11-beta-hydroxysteroid dehydrogenase [Oncorhynchus mykiss] 1144 519506 4.2E-05 -2.9 gi_76445922, ABA42831.1, thiazide sensitive Na-Cl co-transporter, solute carrier family 12, member 3 99 < 1.0E-180 655 [Oncorhynchus mykiss] 1145 694390 4.3E-06 -2.9 gi_410903085, XP_003965024.1, PREDICTED: mpv17-like protein-like [Takifugu rubripes] 92 4.38E-120 364 1146 563524 1.9E-02 -2.9 gi_348536829, XP_003455898.1, PREDICTED: C-type mannose receptor 2-like [Oreochromis niloticus] 58 4.25E-28 116 1147 219748 4.1E-07 -2.9 gi_348533570, XP_003454278.1, PREDICTED: mitochondrial sodium/hydrogen exchanger NHA2-like 86 < 1.0E-180 708 [Oreochromis niloticus] 1148 139733 4.0E-15 -2.9 gi_348506234, XP_003440665.1, PREDICTED: FERM domain-containing protein 5-like [Oreochromis 91 1.67E-94 312 niloticus] 1149 101332 < 1.0E-15 -2.9 gi_209731810, ACI66774.1, Heme-binding protein 2 [Salmo salar] 96 3.37E-144 421 1150 200987 3.7E-09 -3.0 gi_326669021, XP_002662656.2, PREDICTED: hypothetical protein LOC100331153 [Danio rerio] 60 5.71E-07 58 1151 64931 1.4E-02 -3.0 gi_185135940, NP_001117933.1, Na/K ATPase alpha subunit isoform 1a [Oncorhynchus mykiss] 72 < 1.0E-180 481 >gi_34812027, AAQ82790.1, Na/K ATPase alpha subunit isoform 1a [Oncorhynchus mykiss] 1152 657935 3.6E-04 -3.0 gi_123414046, XP_001304408.1, hypothetical protein [Trichomonas vaginalis G3] >gi_121885858, 42 8.50E-06 53 EAX91478.1, hypothetical protein TVAG_384580 [Trichomonas vaginalis G3] 1153 740824 2.2E-02 -3.1 gi_47227825, CAG08988.1, unnamed protein product [Tetraodon nigroviridis] 75 < 1.0E-180 1617 1154 124214 < 1.0E-15 -3.1 gi_209735022, ACI68380.1, Glutathione S-transferase P [Salmo salar] 97 9.66E-96 313 1155 124232 < 1.0E-15 -3.1 gi_209735022, ACI68380.1, Glutathione S-transferase P [Salmo salar] 83 3.51E-61 205 1156 622548 4.3E-06 -3.1 gi_185134400, NP_001117748.1, glucose transporter 1A [Oncorhynchus mykiss] >gi_8489493, 87 < 1.0E-180 786 AAF75681.1, AF247728_1 glucose transporter 1A [Oncorhynchus mykiss] 1157 117908 2.4E-03 -3.1 gi_18157526, BAB83841.1, ReO_6 [Oryzias latipes] 57 5.65E-35 91 1158 161373 3.5E-02 -3.2 gi_41107591, AAH65452.1, Tnnt3b protein [Danio rerio] 90 2.77E-71 233 1159 261113 2.4E-08 -3.2 gi_317419774, CBN81810.1, Putative transposase [Dicentrarchus labrax] 71 2.35E-58 207 1160 220754 1.3E-09 -3.2 gi_308321805, ADO28045.1, prostaglandin reductase 1 [Ictalurus furcatus] 98 4.96E-22 98 1161 755967 4.7E-02 -3.2 gi_213510970, NP_001133114.1, L-lactate dehydrogenase A chain [Salmo salar] >gi_197631979, 99 < 1.0E-180 632 ACH70713.1, lactate dehydrogenase A4 [Salmo salar] 1162 350396 1.8E-04 -3.3 gi_10801662, BAB16748.1, hypothetical protein [Macaca fascicularis] 100 4.72E-20 92 1163 752316 5.3E-02 -3.4 gi_76153153, AAX24812.2, SJCHGC04011 protein [Schistosoma japonicum] 72 8.63E-04 49 1164 136005 1.9E-02 -3.4 gi_391344860, XP_003746712.1, PREDICTED: uncharacterized protein LOC100906026 [Metaseiulus 61 1.68E-08 64 occidentalis] 1165 548032 1.8E-07 -3.4 gi_120300948, NP_001073337.1, orexin receptor type 2 [Danio rerio] >gi_119712738, ABL96925.1, 87 6.29E-123 374 hypocretin receptor [Danio rerio] AC gill Expression BLASTX alignment

Count Contig ID P Log2(ER) Sequence description % ID E-Value Score 1166 759711 1.6E-04 -3.5 gi_348543594, XP_003459268.1, PREDICTED: matrix metalloproteinase-14-like [Oreochromis niloticus] 87 < 1.0E-180 839 1167 64913 3.9E-12 -3.5 gi_185135940, NP_001117933.1, Na/K ATPase alpha subunit isoform 1a [Oncorhynchus mykiss] 99 1.65E-85 242 >gi_34812027, AAQ82790.1, Na/K ATPase alpha subunit isoform 1a [Oncorhynchus mykiss] 1168 529230 1.5E-03 -3.5 gi_156373919, XP_001629557.1, predicted protein [Nematostella vectensis] >gi_156216560, 43 3.84E-07 55 EDO37494.1, predicted protein [Nematostella vectensis] 1169 595825 < 1.0E-15 -3.6 gi_317420106, CBN82142.1, Beta-ketoacyl synthase [Dicentrarchus labrax] 93 < 1.0E-180 611 1170 120156 7.8E-03 -3.7 gi_209731868, ACI66803.1, Troponin I, fast skeletal muscle [Salmo salar] 99 1.98E-102 307 1171 499797 3.1E-02 -3.8 gi_29561775, CAD87780.1, novel protein similar to human titin (TTN) [Danio rerio] 89 2.71E-73 250 1172 117910 7.0E-15 -3.8 gi_18157526, BAB83841.1, ReO_6 [Oryzias latipes] 57 6.23E-35 91 1173 111380 1.5E-03 -3.9 gi_213512959, NP_001133188.1, creatine kinase-2 [Salmo salar] >gi_197632381, ACH70914.1, 97 < 1.0E-180 713 creatine kinase-2 [Salmo salar] 1174 274688 2.3E-03 -4.0 gi_395847864, XP_003796584.1, PREDICTED: unconventional myosin-IXb [Otolemur garnettii] 81 < 1.0E-180 1442 1175 630605 5.1E-03 -4.1 gi_213514108, NP_001134761.1, GTP-binding protein RAY-like [Salmo salar] >gi_209735750, 100 1.50E-125 371 ACI68744.1, GTP-binding protein RAY-like [Salmo salar] 1176 522444 8.2E-05 -4.3 gi_348519867, XP_003447451.1, PREDICTED: cordon-bleu protein-like 1-like [Oreochromis niloticus] 51 1.82E-60 198 1177 7907 < 1.0E-15 -5.8 gi_157841250, NP_001103183.1, G-protein coupled receptor family C group 5 member C precursor 77 8.59E-156 480 [Danio rerio] >gi_156229870, AAI51954.1, Gprc5c protein [Danio rerio]

Percent sequence identity (% ID); P = FDR corrected P-value for differential gene expression; ER = ratio of genes expressed in seawater relative to freshwater.