Difference Between Chemoorganotrophic and Obligate Autotroph
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Prokaryotes (Domains Bacteria & Archaea)
2/4/15 Prokaryotes (Domains Bacteria & Archaea) KEY POINTS 1. Decomposers: recycle organic and inorganic molecules in environment; makes them available to other organisms. 2. Essential components of symbioses. 3. Encompasses the origins of metabolism and metabolic diversity. 4. Origin of photosynthesis and formation of atmospheric Oxygen Ceno- Meso- zoic zoic ANTIQUITY Humans Paleozoic Colonization of land Animals Origin of solar system and Earth • >3.5 BILLION years old. • Alone for 2 1 4 billion years Proterozoic Archaean Prokaryotes Billions of 2 years ago3 Multicellular eukaryotes Single-celled eukaryotes Atmospheric oxygen General characteristics 1. Small: compare to 10-100µm for 0.5-5µm eukaryotic cell; single-celled; may form colonies. 2. Lack membrane- enclosed organelles. 3. Cell wall present, but different from plant cell wall. 1 2/4/15 General characteristics 4. Occur everywhere, most numerous organisms. – More individuals in a handful of soil then there are people that have ever lived. – By far more individuals in our gut than eukaryotic cells that are actually us. General characteristics 5. Metabolic diversity established nutritional modes of eukaryotes. General characteristics 6. Important decomposers and recyclers 2 2/4/15 General characteristics 6. Important decomposers and recyclers • Form the basis of global nutrient cycles. General characteristics 7. Symbionts!!!!!!! • Parasites • Pathogenic organisms. • About 1/2 of all human diseases are caused by Bacteria General characteristics 7. Symbionts!!!!!!! • Parasites • Pathogenic organisms. • Extremely important in agriculture as well. Pierce’s disease is caused by Xylella fastidiosa, a Gamma Proteobacteria. It causes over $56 million in damage annually in California. That’s with $34 million spent to control it! = $90 million in California alone. -
Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas Sp
microorganisms Article Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5 Karel Kopejtka 1 , Yonghui Zeng 1,2, David Kaftan 1,3 , Vadim Selyanin 1, Zdenko Gardian 3,4 , Jürgen Tomasch 5,† , Ruben Sommaruga 6 and Michal Koblížek 1,* 1 Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 379 81 Tˇreboˇn,Czech Republic; [email protected] (K.K.); [email protected] (Y.Z.); [email protected] (D.K.); [email protected] (V.S.) 2 Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark 3 Faculty of Science, University of South Bohemia, 370 05 Ceskˇ é Budˇejovice,Czech Republic; [email protected] 4 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 370 05 Ceskˇ é Budˇejovice,Czech Republic 5 Research Group Microbial Communication, Technical University of Braunschweig, 38106 Braunschweig, Germany; [email protected] 6 Laboratory of Aquatic Photobiology and Plankton Ecology, Department of Ecology, University of Innsbruck, 6020 Innsbruck, Austria; [email protected] * Correspondence: [email protected] † Present Address: Department of Molecular Bacteriology, Helmholtz-Centre for Infection Research, 38106 Braunschweig, Germany. Abstract: An aerobic, yellow-pigmented, bacteriochlorophyll a-producing strain, designated AAP5 Citation: Kopejtka, K.; Zeng, Y.; (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located in the Ty- Kaftan, D.; Selyanin, V.; Gardian, Z.; rolean Alps, Austria. Here, we report its description and polyphasic characterization. Phylogenetic Tomasch, J.; Sommaruga, R.; Koblížek, analysis of the 16S rRNA gene showed that strain AAP5 belongs to the bacterial genus Sphingomonas M. Characterization of the Aerobic and has the highest pairwise 16S rRNA gene sequence similarity with Sphingomonas glacialis (98.3%), Anoxygenic Phototrophic Bacterium Sphingomonas psychrolutea (96.8%), and Sphingomonas melonis (96.5%). -
A Study on the Phototrophic Microbial Mat Communities of Sulphur Mountain Thermal Springs and Their Association with the Endangered, Endemic Snail Physella Johnsoni
A Study on the Phototrophic Microbial Mat Communities of Sulphur Mountain Thermal Springs and their Association with the Endangered, Endemic Snail Physella johnsoni By Michael Bilyj A thesis submitted to the Faculty of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science Department of Microbiology Faculty of Science University of Manitoba Winnipeg, Manitoba October 2011 © Copyright 2011, Michael A. Bilyj 1 Abstract The seasonal population fluctuation of anoxygenic phototrophs and the diversity of cyanobacteria at the Sulphur Mountain thermal springs of Banff, Canada were investigated and compared to the drastic population changes of the endangered snail Physella johnsoni. A new species and two strains of Rhodomicrobium were taxonomically characterized in addition to new species of Rhodobacter and Erythromicrobium. Major mat-forming organisms included Thiothrix-like species, oxygenic phototrophs of genera Spirulina, Oscillatoria, and Phormidium and purple nonsulfur bacteria Rhodobacter, Rhodopseudomonas and Rhodomicrobium. Aerobic anoxygenic phototrophs comprised upwards of 9.6 x 104 CFU/cm2 of mat or 18.9% of total aerobic heterotrophic bacterial isolates at certain sites, while maximal purple nonsulfur and purple sulfur bacteria were quantified at 3.2 x 105 and 2.0 x 106 CFU/cm2 of mat, respectively. Photosynthetic activity measurements revealed incredibly productive carbon fixation rates averaging 40.5 mg C/cm2/24 h. A temporal mismatch was observed for mat area and prokaryote-based organics to P. johnsoni population flux in a ―tracking inertia‖ manner. 2 Acknowledgements It is difficult to express sufficient gratitude to my supervisor Dr. Vladimir Yurkov for his unfaltering patience, generosity and motivation throughout this entire degree. -
ORGANIC GEOCHEMISTRY: CHALLENGES for the 21St CENTURY
ORGANIC GEOCHEMISTRY: CHALLENGES FOR THE 21st CENTURY VOL. 2 Book of Abstracts of the Communications presented to the 22nd International Meeting on Organic Geochemistry Seville – Spain. September 12 -16, 2005 Editors: F.J. González-Vila, J.A. González-Pérez and G. Almendros Equipo de trabajo: Rocío González Vázquez Antonio Terán Rodíguez José Mª de la Rosa Arranz Maquetación: Rocío González Vázquez Fotomecánica e impresión: Akron Gráfica, Sevilla © 22nd IMOG, Sevilla 2005 Depósito legal: SE-61181-2005 I.S.B.N.: 84-689-3661-8 COMMITTEES INVOLVED IN THE ORGANIZATION OF THE 22 IMOG 2005 Chairman: Francisco J. GONZÁLEZ-VILA Vice-Chairman: José A. GONZÁLEZ-PÉREZ Consejo Superior de Investigaciones Científicas (CSIC) Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS) Scientific Committee Francisco J. GONZÁLEZ-VILA (Chairman) IRNAS-CSIC, Spain Gonzalo ALMENDROS Claude LARGEAU CCMA-CSIC, Spain ENSC, France Pim van BERGEN José C. del RÍO SHELL Global Solutions, The Netherlands IRNAS-CSIC, Spain Jørgen A. BOJESEN-KOEFOED Jürgen RULLKÖTTER GEUS, Denmark ICBM, Germany Chris CORNFORD Stefan SCHOUTEN IGI, UK NIOZ, The Netherlands Gary ISAKSEN Eugenio VAZ dos SANTOS NETO EXXONMOBIL, USA PETROBRAS RD, Brazil Local Committee José Ramón de ANDRÉS IGME, Spain Mª Carmen DORRONSORO Mª Enriqueta ARIAS Universidad del País Vasco Universidad de Alcalá Antonio GUERRERO Tomasz BOSKI Universidad de Sevilla Universidad do Algarve, Faro, Portugal Juan LLAMAS Ignacio BRISSON ETSI Minas de Madrid Repsol YPF Albert PERMANYER Juan COTA Universidad de Barcelona Universidad de Sevilla EAOG Board Richard L. PATIENCE (Chairman) Sylvie DERENNE (Secretary) Ger W. van GRAAS (Treasurer) Walter MICHAELIS (Awards) Francisco J. GONZALEZ-VILA (Newsletter) C. -
Identification of Active Methylotroph Populations in an Acidic Forest Soil
Microbiology (2002), 148, 2331–2342 Printed in Great Britain Identification of active methylotroph populations in an acidic forest soil by stable- isotope probing Stefan Radajewski,1 Gordon Webster,2† David S. Reay,3‡ Samantha A. Morris,1 Philip Ineson,4 David B. Nedwell,3 James I. Prosser2 and J. Colin Murrell1 Author for correspondence: J. Colin Murrell. Tel: j44 24 7652 2553. Fax: j44 24 7652 3568. e-mail: cmurrell!bio.warwick.ac.uk 1 Department of Biological Stable-isotope probing (SIP) is a culture-independent technique that enables Sciences, University of the isolation of DNA from micro-organisms that are actively involved in a Warwick, Coventry CV4 7AL, UK specific metabolic process. In this study, SIP was used to characterize the active methylotroph populations in forest soil (pH 35) microcosms that were exposed 2 Department of Molecular 13 13 13 13 and Cell Biology, to CH3OH or CH4. Distinct C-labelled DNA ( C-DNA) fractions were resolved University of Aberdeen, from total community DNA by CsCl density-gradient centrifugation. Analysis of Institute of Medical 16S rDNA sequences amplified from the 13C-DNA revealed that bacteria related Sciences, Foresterhill, Aberdeen AB25 2ZD, UK to the genera Methylocella, Methylocapsa, Methylocystis and Rhodoblastus had assimilated the 13C-labelled substrates, which suggested that moderately 3 Department of Biological Sciences, University of acidophilic methylotroph populations were active in the microcosms. Essex, Wivenhoe Park, Enrichments targeted towards the active proteobacterial CH3OH utilizers were Colchester, Essex CO4 3SQ, successful, although none of these bacteria were isolated into pure culture. A UK parallel analysis of genes encoding the key enzymes methanol dehydrogenase 4 Department of Biology, and particulate methane monooxygenase reflected the 16S rDNA analysis, but University of York, PO Box 373, YO10 5YW, UK unexpectedly revealed sequences related to the ammonia monooxygenase of ammonia-oxidizing bacteria (AOB) from the β-subclass of the Proteobacteria. -
Thermophilic Lithotrophy and Phototrophy in an Intertidal, Iron-Rich, Geothermal Spring 2 3 Lewis M
bioRxiv preprint doi: https://doi.org/10.1101/428698; this version posted September 27, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Thermophilic Lithotrophy and Phototrophy in an Intertidal, Iron-rich, Geothermal Spring 2 3 Lewis M. Ward1,2,3*, Airi Idei4, Mayuko Nakagawa2,5, Yuichiro Ueno2,5,6, Woodward W. 4 Fischer3, Shawn E. McGlynn2* 5 6 1. Department of Earth and Planetary Sciences, Harvard University, Cambridge, MA 02138 USA 7 2. Earth-Life Science Institute, Tokyo Institute of Technology, Meguro, Tokyo, 152-8550, Japan 8 3. Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 9 91125 USA 10 4. Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, 11 Japan 12 5. Department of Earth and Planetary Sciences, Tokyo Institute of Technology, Meguro, Tokyo, 13 152-8551, Japan 14 6. Department of Subsurface Geobiological Analysis and Research, Japan Agency for Marine-Earth 15 Science and Technology, Natsushima-cho, Yokosuka 237-0061, Japan 16 Correspondence: [email protected] or [email protected] 17 18 Abstract 19 Hydrothermal systems, including terrestrial hot springs, contain diverse and systematic 20 arrays of geochemical conditions that vary over short spatial scales due to progressive interaction 21 between the reducing hydrothermal fluids, the oxygenated atmosphere, and in some cases 22 seawater. At Jinata Onsen, on Shikinejima Island, Japan, an intertidal, anoxic, iron- and 23 hydrogen-rich hot spring mixes with the oxygenated atmosphere and sulfate-rich seawater over 24 short spatial scales, creating an enormous range of redox environments over a distance ~10 m. -
Adaptive Laboratory Evolution Enhances Methanol Tolerance and Conversion in Engineered Corynebacterium Glutamicum
ARTICLE https://doi.org/10.1038/s42003-020-0954-9 OPEN Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum Yu Wang 1, Liwen Fan1,2, Philibert Tuyishime1, Jiao Liu1, Kun Zhang1,3, Ning Gao1,3, Zhihui Zhang1,3, ✉ ✉ 1234567890():,; Xiaomeng Ni1, Jinhui Feng1, Qianqian Yuan1, Hongwu Ma1, Ping Zheng1,2,3 , Jibin Sun1,3 & Yanhe Ma1 Synthetic methylotrophy has recently been intensively studied to achieve methanol-based biomanufacturing of fuels and chemicals. However, attempts to engineer platform micro- organisms to utilize methanol mainly focus on enzyme and pathway engineering. Herein, we enhanced methanol bioconversion of synthetic methylotrophs by improving cellular tolerance to methanol. A previously engineered methanol-dependent Corynebacterium glutamicum is subjected to adaptive laboratory evolution with elevated methanol content. Unexpectedly, the evolved strain not only tolerates higher concentrations of methanol but also shows improved growth and methanol utilization. Transcriptome analysis suggests increased methanol con- centrations rebalance methylotrophic metabolism by down-regulating glycolysis and up- regulating amino acid biosynthesis, oxidative phosphorylation, ribosome biosynthesis, and parts of TCA cycle. Mutations in the O-acetyl-L-homoserine sulfhydrylase Cgl0653 catalyzing formation of L-methionine analog from methanol and methanol-induced membrane-bound transporter Cgl0833 are proven crucial for methanol tolerance. This study demonstrates the importance of -
Aerobic Respiration
Life is based on redox • All energy generation in biological systems is due to redox (reduction-oxidation) reactions Aerobic Respiration: + - C6H12O6 + 6 H2O ==> 6 CO2 + 24 H +24 e oxidation electron donor (aka energy source) + - (O2+ 4H + 4e ==> 2H2O) x6 reduction electron acceptor --------------------------------------- C6H12O6 + 6 O2 ==> 6 CO2 + 6 H2O overall reaction (24 electrons) Types of bacterial metabolisms • While eukaryotes only reduce O2 and oxidize organic compounds, prokaryotes can use a variety of electron donors and acceptors, organic and inorganic. - • Aerobic respiration: e acceptor is O2 - • Anaerobic respiration: e acceptor is not O2 • Fermentation: e- donor and acceptor are organic molecules • Chemolithotrophy: e- donor and acceptor are inorganic molecules • Phototrophy: e- donor is light and e- acceptor is either organic or inorganic all microorganisms energy source? chemical light chemotroph phototroph carbon source? carbon source? organic organic CO CO compound 2 compound 2 chemoheterotroph chemoautotroph photoheterotroph photoautotroph e- acceptor? Nitrifying and sulfur- use H O to reduce CO ? oxidizing bacteria 2 2 green non-sulfur and O Other than O 2 2 purple non-sulfur bacteria anoxygenic oxygenic photosynthesis: photosynthesis: green sulfur and most bacteria Organic Inorganic cyanobacteria compound compound purple sulfur bacteria fermentative organism anaerobic respiration: nitrate, sulfate, Fe(III) Aerobic or anaerobic respiration Chemolithotrophy Important molecules Redox Electron Carrier: for example the -
Nitrogen and Phosphorus Limitation of Oceanic Microbial Growth During Spring in the Gulf of Aqaba
Vol. 56: 227–239, 2009 AQUATIC MICROBIAL ECOLOGY Printed September 2009 doi: 10.3354/ame01357 Aquat Microb Ecol Published online August 27, 2009 Contribution to AME Special 2 ‘Progress and perspectives in aquatic primary productivity’ OPEN ACCESS Nitrogen and phosphorus limitation of oceanic microbial growth during spring in the Gulf of Aqaba David J. Suggett1,*, Noga Stambler2, Ondrej Prá$il3, Zbigniew Kolber4, Antonietta Quigg5, Evaristo Vázquez-Domínguez6, Tamar Zohary7, Tom Berman7, David Iluz8, Orly Levitan2, Tracy Lawson1, Efrat Meeder9,10, Boaz Lazar9,10, Edo Bar-Zeev2, Hana Medova3, Ilana Berman-Frank8 1Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, UK 2Department of Geography and Environment, Bar-Ilan University, Ramat-Gan 52900, Israel 3Photosynthesis Laboratory, Institute of Microbiology ASCR, Opatovicky mlyn, 379 81 Trˇ ebonˇ, Czech Republic 4Monterey Bay Aquarium Research Institute, 7700 Sandholdt Road, Moss Landing, California 95039, USA 5Department of Marine Biology and Oceanography, Texas A&M University, Galveston, Texas 77551, USA 6CSIC, Institut de Ciències del Mar, Passeig Marítim de la Barceloneta 39-43, 08003 Barcelona, Spain 7Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, PO Box 447, Migdal 14950, Israel 8The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel 9The Interuniversity Institute for Marine Sciences Coral Beach, PO Box 469, 88103 Eilat, Israel 10Institute of Earth Sciences, The Hebrew University Edmond J. Safra Campus, Jerusalem 91904, Israel ABSTRACT: Bioassay experiments were performed to identify how growth of key groups within the mi- crobial community was simultaneously limited by nutrient (nitrogen and phosphorus) availability during spring in the Gulf of Aqaba’s oceanic waters. -
IB HL Biology: Ecology Review Fall 2017 Populations 1. Define the Following Terms Associated with Population Ecology; Population and Carrying Capacity
IB HL Biology: Ecology Review Fall 2017 Populations 1. Define the following terms associated with population ecology; population and carrying capacity. 2. What processes contribute to changes in population size? 3. What are some factors which can increase the carrying capacity of a population? Decrease? 4. What is quadrat sampling? When would it be used? Communities 5. Define the following terms; community, autotroph, heterotroph, producer, primary consumer, secondary consumer, detritivore and saprotroph. 6. What is the initial energy source for all communities? 7. Be able to read food webs and determine the trophic level of different species. 8. Choose 2 regions below and determine the Simpson’s Diversity Index value for these regions. Which region is more diverse? A. An area of the Black Forest in Germany contains 134 pitch pines, 24 douglas firs, and 53 red pines. B. A meadow contains 1532 chestnut oaks, 342 black cherry trees, 12 white ash trees, and 1022 yellow birches. C. You school science classroom contains 12 beetles, 34 termites, 84 ants, 93 fleas, and 1 butterfly. D. An African park contains 15 lions, 94 giraffes, 1000 wildebeests, 50 elephants, and 5 hyenas. Choose more areas if you need more practice. 9. What is a keystone species? 10. Distinguish between primary and secondary succession. Ecosystems 11. What is an ecosystem? 12. Explain the 10% rule of energy transfer. How is the energy lost between trophic levels? 13. Review the Carbon Cycle. What are the main sources of carbon dioxide on earth? 14. Review the Nitrogen Cycle. 15. Distinguish between Gross Primary Productivity and Net Primary Productivity. -
Chapter 11 – PROKARYOTES: Survey of the Bacteria & Archaea
Chapter 11 – PROKARYOTES: Survey of the Bacteria & Archaea 1. The Bacteria 2. The Archaea Important Metabolic Terms Oxygen tolerance/usage: aerobic – requires or can use oxygen (O2) anaerobic – does not require or cannot tolerate O2 Energy usage: autotroph – uses CO2 as a carbon source • photoautotroph – uses light as an energy source • chemoautotroph – gets energy from inorganic mol. heterotroph – requires an organic carbon source • chemoheterotroph – gets energy & carbon from organic molecules …more Important Terms Facultative vs Obligate: facultative – “able to, but not requiring” e.g. • facultative anaerobes – can survive w/ or w/o O2 obligate – “absolutely requires” e.g. • obligate anaerobes – cannot tolerate O2 • obligate intracellular parasite – can only survive within a host cell The 2 Prokaryotic Domains Overview of the Bacterial Domain We will look at examples from several bacterial phyla grouped largely based on rRNA (ribotyping): Gram+ bacteria • Firmicutes (low G+C), Actinobacteria (high G+C) Proteobacteria (Gram- heterotrophs mainly) Gram- nonproteobacteria (photoautotrophs) Chlamydiae (no peptidoglycan in cell walls) Spirochaetes (coiled due to axial filaments) Bacteroides (mostly anaerobic) 1. The Gram+ Bacteria Gram+ Bacteria The Gram+ bacteria are found in 2 different phyla: Firmicutes • low G+C content (usually less than 50%) • many common pathogens Actinobacteria • high G+C content (greater than 50%) • characterized by branching filaments Firmicutes Characteristics associated with this phylum: • low G+C Gram+ bacteria -
Nicole Kube Phd
Leibniz-Institut für Meereswissenschaften The integration of microalgae photobioreactors in a recirculation system for low water discharge mariculture Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultät an der Christian-Albrechts-Universität zu Kiel vorgelegt von Nicole Kube Kiel, 2006 Referentin: Prof. Dr. Karin Lochte Koreferent: Prof. Dr. Dr. h.c. Harald Rosenthal Tag der mündlichen Prüfung: Zum Druck genehmigt: Kiel, den Der Dekan Foreword The manuscripts included in this thesis are prepared for submission to peer- reviewed journals as listed below: Wecker B., Kube N., Bischoff A.A., Waller U. (2006). MARE – Marine Artificial Recirculated Ecosystem: feasibility and modelling of a novel integrated recirculation system. (manuscript) Kube N., Bischoff A.A., Wecker B., Waller U. Cultivation of microalgae using a continuous photobioreactor system based on dissolved nutrients of a recirculation system for low water discharge mariculture (manuscript) Kube N. And Rosenthal H. Ozonation and foam fractionation used for the removal of bacteria and parti- cles in a marine recirculation system for microalgae cultivation (manuscript) Kube N., Bischoff A.A., Blümel M., Wecker B., Waller U. MARE – Marine Artificial Recirulated Ecosystem II: Influence on the nitrogen cycle in a marine recirculation system with low water discharge by cultivat- ing detritivorous organisms and phototrophic microalgae. (manuscript) This thesis has been realised with the help of several collegues. The contributions in particular