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Prokaryotes (Domains Bacteria & Archaea) KEY POINTS 1. Decomposers: recycle organic and inorganic molecules in environment; makes them available to other organisms. 2. Essential components of symbioses. 3. Encompasses the origins of metabolism and metabolic diversity. 4. Origin of photosynthesis and formation of atmospheric Oxygen
Ceno- Meso- zoic zoic ANTIQUITY Humans Paleozoic Colonization of land
Animals Origin of solar system and Earth • >3.5 BILLION years old.
• Alone for 2 1 4
billion years Proterozoic Archaean Prokaryotes Billions of
2 years ago3 Multicellular eukaryotes
Single-celled eukaryotes Atmospheric oxygen
General characteristics
1. Small: compare to 10-100µm for 0.5-5µm eukaryotic cell; single-celled; may form colonies. 2. Lack membrane- enclosed organelles. 3. Cell wall present, but different from plant cell wall.
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General characteristics
4. Occur everywhere, most numerous organisms. – More individuals in a handful of soil then there are people that have ever lived. – By far more individuals in our gut than eukaryotic cells that are actually us.
General characteristics
5. Metabolic diversity established nutritional modes of eukaryotes.
General characteristics
6. Important decomposers and recyclers
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General characteristics
6. Important decomposers and recyclers • Form the basis of global nutrient cycles.
General characteristics
7. Symbionts!!!!!!! • Parasites • Pathogenic organisms. • About 1/2 of all human diseases are caused by Bacteria
General characteristics
7. Symbionts!!!!!!! • Parasites • Pathogenic organisms. • Extremely important in agriculture as well. Pierce’s disease is caused by Xylella fastidiosa, a Gamma Proteobacteria. It causes over $56 million in damage annually in California. That’s with $34 million spent to control it! = $90 million in California alone.
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General characteristics
7. Symbionts!!!!!!! • Commensalists • They are everywhere (really). • There can be 10 million cells per square centimeter of skin.
General characteristics
7. Symbionts!!!!!!! • Mutualists • Eukaryotic life would be impossible without this.
General characteristics
7. Symbionts!!!!!!! • Mutualists • Allows herbivorous (plant-feeding) animals to digest cellulose and other low-quality plant tissues. • Termites • Ungulates “chewing the cud” • Lagomorph coprophagy
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General characteristics
7. Symbionts!!!!!!! • Mutualists • Mealybug endosymbionts with endosymbionts.
General characteristics 7. Symbionts!!!!!!! • Mutualists • Komodo dragons and their “toxins”. • Hunt large prey and can inflict fairly minor wound. • Prey die fairly quickly from wound. • Infection by highly pathogenic Pasteurella multocida (Gamma Proteobacteria). • Prominent in saliva of dragons, but dragons have an anti- Pasteurella antibody.
TAXONOMY is problematic
• Relationships obscured by billions of years of evolution • Also obscured by unique bacterial means of recombination (more later). • Grouped primarily by DNA sequence data. • Immense genetic/genomic diversity.
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Current taxonomy is stabilizing • Note that “Prokaryote” is paraphyletic. Why? • Two Domains: • Archaea: extremophiles (mostly), ancient, probable progenitors of eukaryotes. • Bacteria: most commonly-encountered prokaryotes.
Characteristics
• Cell Surface • Motility • Genome • Reproduction & Growth • Metabolic Diversity • Nitrogen Metabolism • Oxygen Relationships
Cell Surface
• Archaea: plasma membrane of ether-lipids (unique in life). • Bacteria: a sugar polymer - peptidoglycan
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Cell Surface
• Cell wall is often modified with structures to adhere to substrate. • Many secrete a sticky capsule or adhere by fimbriae (ocasionally called pili).
Motility
• ~half the species can move. 1. Flagella most common (different structure from eukaryote) 2. Spiral filaments: spirochetes corkscrew 3. Gliding over slimy secretions (via flagellar motors without filament) • Capable of taxis (photo, chemo, geo, etc.)
Genome
• Small genomes: ~1/1000th DNA content of eukaryotes. • No membrane enclosed nucleus. • DNA concentrated in ‘nucleoid’ region.
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Genome
• One major chromosome, double stranded DNA molecule in ring. • Sometimes several small DNA rings of few genes: plasmids. – Replicate independently of main chromosome. – Permit recombination via conjugation (later). – Involved in resistance to antibodies/antibiotics.
Genome
• Broadly, replication & translation of genetic info like that of eukaryotes; differ in details and simplicity. • Used in first DNA recombinant research. • Genetic recombination: Not like eukaryotes (e.g. chiasma & crossing over)!! – Transformation – Conjugation – Transduction
Genome: Recombination via transformation • DNA taken up from the environment
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Genome: Recombination via conjugation • Direct transfer of DNA between cells. • Both plasmids and portions of bacterial chromosome.
Genome: Recombination via transduction • Transfer of DNA via phage viruses.
Reproduction & Growth
• Meiosis & Mitosis NOT PRESENT. • Asexual binary fission. • DNA replication can be nearly continuous in ideal conditions (depends on pH, salinity, temperature, etc.) • Generation times as fast as 20 minutes
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Metabolism Metabolic Diversity
• Nutrition: • Requires a source of carbon for synthesizing organic compounds: either carbon dioxide or living matter. • Requires a source of energy to drive reactions: either light or chemical.
Metabolic Diversity: Metabolism Source of Carbon
• AUTOTROPHS: Need only carbon
dioxide (CO2) as carbon source • HETEROTROPHS: Need at least one organic nutrient as carbon source (e.g. glucose; petroleum)
• Both of these present in domain Eucarya as well.
Metabolic Diversity: Metabolism Source of Energy
• PHOTOTROPHS: Need only sunlight as energy source • CHEMOTROPHS: Derive energy from oxidation of organic molecules.
• Both of these present in domain Eucarya as well.
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Metabolism Metabolic Diversity: Combined
Energy Source:
Sun Environment
Photoautotroph Chemoautotroph CO2 Source: Source: Carbon Organic Photoheterotroph Chemoheterotroph molecules Which of these are present in multicellular Eucarya?
Metabolism Photoautotrophs
• Use sun for energy,
CO2 for carbon. • Photosynthetic bacteria (e.g. cyanobacteria). • Present in many plants and single- celled Eucarya
Metabolism Chemoautotrophs • Oxidize inorganics (H2S, NH3) for energy.
• Need only CO2 as carbon source. • Unique to Bacteria and Archaea. • E.g. Methanococcus jannaschii lives on hydrothermal vents at 2600m below sea level.
• Reduces H2 + CO2 to CH4 + 2H2O.
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Metabolism Photoheterotrophs
• Get enery from light but must obtain carbon in organic
form (NOT CO2). • Unique to Bacteria and Archaea. • E.g. Halobacterium salinarium.
Metabolism Chemoheterotrophs • Consume organic molecules for both energy and carbon. • Common among prokaryotes: – saprobes (decomposers) – parasites (rely on living hosts) • Also widespread in Protista, Animalia, Plantae.
Metabolism Nitrogen metabolism
• Nitrogen fixation: • The only* mechanism that makes atmospheric Nitrogen available to other organisms.
• Convert N2 into ammonia (NH3) which is quickly protonated into + ammonium (NH4 ). • Essential for multicellular life!
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Oxygen Relationships
• Aerobic vs. Anaerobic • Cellular respiration:
• Obligate aerobes: use O2 for – Carbohydrates + O2 →CO2 cellular respiration. + H2O + energy • Facultative anaerobes: use • Fermentation: O2 if it is present by carry out – Carbohydrates →CO2 + anaerobic respiration or ethanol + energy fermentation in anaerobic • Anaerobic respiration: environment. – Carbohydrates + [X] → • Obligate anaerobes: bicarbonate + [X-] + energy poisoned by O2; use – Where [X] is a substance anaerobic respiration or other than O2 that accepts fermentation. electrons such as nitrates or sulfates
Oxygen Relationships
• Early life (during the Archaean) was primarily anaerobic. • Evolution of photosynthesis in Cyanobacteria changed all this.
Taxonomy of Prokaryotes
• Archaea or Archaebacteria – Methanogens – Halophiles – Thermophiles • Bacteria or Eubacteria – Protobacteria – Chlamyidias – Spirochetes – Cyanobacteria – Gram-positive bacteria
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Archaea or Archaebacteria
• Live in extreme environments (extremophiles): sulfur hot springs, deep sea vents, high salt environments. • Lack peptidoglycan, unique plasma membrane of liquids • Likely sister group of Eukaryotes
Archaea or Archaebacteria
• Methanogens
– Use H2 to reduce CO2 to methane (CH4). – Chemoautotrophs – Anaerobic – In swamps, marshes, deep sea vents, important decomposers.
Archaea or Archaebacteria
• Halophiles – Saline environments. – Salinity several times higher than sea water. – Photoheterotrophs
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Archaea or Archaebacteria
• Thermophiles – 60º-80ºCpH 2-4 optimal – Chemoautotrophs – Oxidize HS
Bacteria or Eubacteria
• Grouped by molecular systematics.
Bacteria or Eubacteria
Proteobacteria • VERY diverse, grouped into five taxa based on DNA sequence data. • Includes most types of metabolism that we’ve discussed. • Includes most of the types of symbioses we’ve discussed • Review the summary in Figure 27.16.
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Bacteria or Eubacteria Gram positive Bacteria: • Simple peptidoglycan cell wall. • Rival Proteobacteria in diversity. • Most are free-living decomposers. • Some pathogenic (e.g. strains of Staphylococcus, Streptococcus, Bacillus anthracis, Clostridium botulinum). • Include the mycoplasms--the only bacteria that lack cell walls
Bacteria or Eubacteria Cyanobacteria: • Photoautotrophs • Only prokaryotes with plant-like, O2-generating photosynthesis. • Present in freshwater and marine environments. • Often colonial--first steps toward multicellularity?
Bacteria or Eubacteria
Spirochetes: • Helical • Recall motility: move by means of rotating, internal, flagellum-like filaments. • Free-living and parasitic. • Chemoheterotrophs (like us).
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Bacteria or Eubacteria
Chlamydias: • ALL are parasites of animals. • Intercellular. • Lack peptidoglycan in the cell wall (are they gram-positive or gram-negative?). • Most common form of STD in USA (urethritis).
Prokaryotes: Summary
• You should now have a good sense of prokaryote biology and diversity. • Including roles in metabolism, symbioses, global energy cycles. • Important distinguishing characteristics of cell wall, motility, genome, replication. • General aspects of their systematics.
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