A Day in the Life of an Animal
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National Resource Material Green and Black Poison Frog (Dendrobates Auratus)
Indicative 10 Project National Resource Material Green and Black Poison frog (Dendrobates auratus) Michelle T. Christy and Win Kirkpatrick 2017 Department of Primary Industries and Regional Development 3 Baron-Hay Court, South Perth, WA 6151 An Invasive Animals CRC Project Contents Summary ............................................................................. 2 Key Messages ................................................................... 2 Classification ................................................................... 2 Common names ................................................................ 3 Biology and Ecology ................................................................ 3 Identification ................................................................... 3 Behaviours and Traits ......................................................... 4 Food and Foraging ............................................................. 4 Reproduction and Lifecycle ................................................. 5 Habitat ......................................................................... 5 Global Range ........................................................................ 5 Potential for Introduction ........................................................ 6 Potential for Eradication.......................................................... 7 Impacts ............................................................................... 7 Economic ........................................................................ 7 Environmental -
Ecol 483/583 – Herpetology Lab 3: Amphibian Diversity 2: Anura Spring 2010
Ecol 483/583 – Herpetology Lab 3: Amphibian Diversity 2: Anura Spring 2010 P.J. Bergmann & S. Foldi (Modified from Bonine & Foldi 2008) Lab objectives The objectives of today’s lab are to: 1. Familiarize yourself with Anuran diversity. 2. Learn to identify local frogs and toads. 3. Learn to use a taxonomic key. Today’s lab is the second in which you will learn about amphibian diversity. We will cover the Anura, or frogs and toads, the third and final clade of Lissamphibia. Tips for learning the material Continue what you have been doing in previous weeks. Examine all of the specimens on display, taking notes, drawings and photos of what you see. Attempt to identify the local species to species and the others to their higher clades. Quiz each other to see which taxa are easy for you and which ones give you troubles, and then revisit the difficult ones. Although the Anura has a conserved body plan – all are rather short and rigid bodied, with well- developed limbs, there is an incredible amount of diversity. Pay close attention to some of the special external anatomical traits that characterize the groups of frogs you see today. You will also learn to use a taxonomic key today. This is an important tool for correctly identifying species, especially when they are very difficult to distinguish from other species. 1 Ecol 483/583 – Lab 3: Anura 2010 Exercise 1: Anura diversity General Information Frogs are a monophyletic group comprising the order Anura. Salientia includes both extant and extinct frogs. Frogs have been around since the Triassic (~230 ma). -
A New Family of Diverse Skin Peptides from the Microhylid Frog Genus Phrynomantis
molecules Article A New Family of Diverse Skin Peptides from the Microhylid Frog Genus Phrynomantis Constantijn Raaymakers 1,2, Benoit Stijlemans 3,4, Charlotte Martin 5, Shabnam Zaman 1 , Steven Ballet 5, An Martel 2, Frank Pasmans 2 and Kim Roelants 1,* 1 Amphibian Evolution Lab, Biology Department, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Elsene, Belgium; [email protected] (C.R.); [email protected] (S.Z.) 2 Wildlife Health Ghent, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium; [email protected] (A.M.); [email protected] (F.P.) 3 Unit of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Elsene, Belgium; [email protected] 4 Myeloid Cell Immunology Lab, VIB Centre for Inflammation Research, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Elsene, Belgium 5 Research Group of Organic Chemistry, Department of Chemistry and Department of Bio-engineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Elsene, Belgium; [email protected] (C.M.); [email protected] (S.B.) * Correspondence: [email protected]; Tel.: +32-26293410 Received: 17 January 2020; Accepted: 18 February 2020; Published: 18 February 2020 Abstract: A wide range of frogs produce skin poisons composed of bioactive peptides for defence against pathogens, parasites and predators. While several frog families have been thoroughly screened for skin-secreted peptides, others, like the Microhylidae, have remained mostly unexplored. Previous studies of microhylids found no evidence of peptide secretion, suggesting that this defence adaptation was evolutionarily lost. We conducted transcriptome analyses of the skins of Phrynomantis bifasciatus and Phrynomantis microps, two African microhylid species long suspected to be poisonous. -
615.9Barref.Pdf
INDEX Abortifacient, abortifacients bees, wasps, and ants ginkgo, 492 aconite, 737 epinephrine, 963 ginseng, 500 barbados nut, 829 blister beetles goldenseal blister beetles, 972 cantharidin, 974 berberine, 506 blue cohosh, 395 buckeye hawthorn, 512 camphor, 407, 408 ~-escin, 884 hypericum extract, 602-603 cantharides, 974 calamus inky cap and coprine toxicity cantharidin, 974 ~-asarone, 405 coprine, 295 colocynth, 443 camphor, 409-411 ethanol, 296 common oleander, 847, 850 cascara, 416-417 isoxazole-containing mushrooms dogbane, 849-850 catechols, 682 and pantherina syndrome, mistletoe, 794 castor bean 298-302 nutmeg, 67 ricin, 719, 721 jequirity bean and abrin, oduvan, 755 colchicine, 694-896, 698 730-731 pennyroyal, 563-565 clostridium perfringens, 115 jellyfish, 1088 pine thistle, 515 comfrey and other pyrrolizidine Jimsonweed and other belladonna rue, 579 containing plants alkaloids, 779, 781 slangkop, Burke's, red, Transvaal, pyrrolizidine alkaloids, 453 jin bu huan and 857 cyanogenic foods tetrahydropalmatine, 519 tansy, 614 amygdalin, 48 kaffir lily turpentine, 667 cyanogenic glycosides, 45 lycorine,711 yarrow, 624-625 prunasin, 48 kava, 528 yellow bird-of-paradise, 749 daffodils and other emetic bulbs Laetrile", 763 yellow oleander, 854 galanthamine, 704 lavender, 534 yew, 899 dogbane family and cardenolides licorice Abrin,729-731 common oleander, 849 glycyrrhetinic acid, 540 camphor yellow oleander, 855-856 limonene, 639 cinnamomin, 409 domoic acid, 214 rna huang ricin, 409, 723, 730 ephedra alkaloids, 547 ephedra alkaloids, 548 Absorption, xvii erythrosine, 29 ephedrine, 547, 549 aloe vera, 380 garlic mayapple amatoxin-containing mushrooms S-allyl cysteine, 473 podophyllotoxin, 789 amatoxin poisoning, 273-275, gastrointestinal viruses milk thistle 279 viral gastroenteritis, 205 silibinin, 555 aspartame, 24 ginger, 485 mistletoe, 793 Medical Toxicology ofNatural Substances, by Donald G. -
A Review of Chemical Defense in Poison Frogs (Dendrobatidae): Ecology, Pharmacokinetics, and Autoresistance
Chapter 21 A Review of Chemical Defense in Poison Frogs (Dendrobatidae): Ecology, Pharmacokinetics, and Autoresistance Juan C. Santos , Rebecca D. Tarvin , and Lauren A. O’Connell 21.1 Introduction Chemical defense has evolved multiple times in nearly every major group of life, from snakes and insects to bacteria and plants (Mebs 2002 ). However, among land vertebrates, chemical defenses are restricted to a few monophyletic groups (i.e., clades). Most of these are amphibians and snakes, but a few rare origins (e.g., Pitohui birds) have stimulated research on acquired chemical defenses (Dumbacher et al. 1992 ). Selective pressures that lead to defense are usually associated with an organ- ism’s limited ability to escape predation or conspicuous behaviors and phenotypes that increase detectability by predators (e.g., diurnality or mating calls) (Speed and Ruxton 2005 ). Defended organisms frequently evolve warning signals to advertise their defense, a phenomenon known as aposematism (Mappes et al. 2005 ). Warning signals such as conspicuous coloration unambiguously inform predators that there will be a substantial cost if they proceed with attack or consumption of the defended prey (Mappes et al. 2005 ). However, aposematism is likely more complex than the simple pairing of signal and defense, encompassing a series of traits (i.e., the apose- matic syndrome) that alter morphology, physiology, and behavior (Mappes and J. C. Santos (*) Department of Zoology, Biodiversity Research Centre , University of British Columbia , #4200-6270 University Blvd , Vancouver , BC , Canada , V6T 1Z4 e-mail: [email protected] R. D. Tarvin University of Texas at Austin , 2415 Speedway Stop C0990 , Austin , TX 78712 , USA e-mail: [email protected] L. -
Regulation of Alkaloid Biosynthesis in Plants
CONTRIBUTORS Numbers in parentheses indicate the pages on which the authors’ contributions begin. JAUME BASTIDA (87), Departament de Productes Naturals, Facultat de Farma` cia, Universitat de Barcelona, 08028 Barcelona, Spain YEUN-MUN CHOO (181), Department of Chemistry, University of Malaya, 50603 Kuala Lumpur, Malaysia PETER J. FACCHINI (1), Department of Biological Sciences, University of Calgary, Calgary, AB, Canada TOH-SEOK KAM (181), Department of Chemistry, University of Malaya, 50603 Kuala Lumpur, Malaysia RODOLFO LAVILLA (87), Parc Cientı´fic de Barcelona, Universitat de Barcelona, 08028 Barcelona, Spain DANIEL G. PANACCIONE (45), Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506-6108, USA CHRISTOPHER L. SCHARDL (45), Department of Plant Pathology, University of Kentucky, Lexington, KY 40546-0312, USA PAUL TUDZYNSKI (45), Institut fu¨r Botanik, Westfa¨lische Wilhelms Universita¨tMu¨nster, Mu¨nster D-48149, Germany FRANCESC VILADOMAT (87), Departament de Productes Naturals, Facultat de Farma` cia, Universitat de Barcelona, 08028 Barcelona, Spain vii PREFACE This volume of The Alkaloids: Chemistry and Biology is comprised of four very different chapters; a reflection of the diverse facets that comprise the study of alkaloids today. As awareness of the global need for natural products which can be made available as drugs on a sustainable basis increases, so it has become increas- ingly important that there is a full understanding of how key metabolic pathways can be optimized. At the same time, it remains important to find new biologically active alkaloids and to elucidate the mechanisms of action of those that do show potentially useful or novel biological effects. Facchini, in Chapter 1, reviews the significant studies that have been conducted with respect to how the formation of alkaloids in their various diverse sources are regulated at the molecular level. -
Rampant Tooth Loss Across 200 Million Years of Frog Evolution
bioRxiv preprint doi: https://doi.org/10.1101/2021.02.04.429809; this version posted February 6, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Rampant tooth loss across 200 million years of frog evolution 2 3 4 Daniel J. Paluh1,2, Karina Riddell1, Catherine M. Early1,3, Maggie M. Hantak1, Gregory F.M. 5 Jongsma1,2, Rachel M. Keeffe1,2, Fernanda Magalhães Silva1,4, Stuart V. Nielsen1, María Camila 6 Vallejo-Pareja1,2, Edward L. Stanley1, David C. Blackburn1 7 8 1Department of Natural History, Florida Museum of Natural History, University of Florida, 9 Gainesville, Florida USA 32611 10 2Department of Biology, University of Florida, Gainesville, Florida USA 32611 11 3Biology Department, Science Museum of Minnesota, Saint Paul, Minnesota USA 55102 12 4Programa de Pós Graduação em Zoologia, Universidade Federal do Pará/Museu Paraense 13 Emilio Goeldi, Belém, Pará Brazil 14 15 *Corresponding author: Daniel J. Paluh, [email protected], +1 814-602-3764 16 17 Key words: Anura; teeth; edentulism; toothlessness; trait lability; comparative methods 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.04.429809; this version posted February 6, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. -
Poison Dart Frogs
POISON DART FROGS Anura Dendrobates tinctorius Family: Dendrobatidae Genus: multiple Range: Southern Central America and north and central South America Habitat: tropical rainforests Niche: Diurnal, terrestrial; breed in trees, carnivorous Wild diet: small invertebrates, particularly ants, which give them their poisonous properties in most cases Zoo diet: pinhead crickets, fruitflies Life Span: (Wild) 3-15 years (Captivity) up to 20 years Sexual dimorphism: Males slightly smaller Location in SF Zoo: South American Tropical Rainforest and Aviary APPEARANCE & PHYSICAL ADAPTATIONS: There are 40 species of Dendrobates poison dart frogs. All have bright coloration (aposematic coloration), which warns predators of their toxic skin secretions (alkaloids obtained from insects they eat). They are small frogs (most are no bigger than a paper clip). They have a good vision used to help capture prey. Their long, sticky tongue darts out and captures their prey once spoted. Each foot contains four toes which each have a flattened tip with a suction cup pad which is used for gripping and clinging to vegetation in its habitat. They lack webbing and are poor swimmers and are found near water but not in it. Weight: < 1 oz (< 28 g) Poison Dart Frogs have no webbing between the toes on their feet, so Length: 1 - 6 cm they are poor swimmers and are not often found in the water. STATUS & CONSERVATION: Many species are threatened by habitat loss and over-collection for the pet trade. COMMUNICATION AND OTHER BEHAVIOR The males are territorial, calling to advertise to females and to defend their area. Calls are species dependent and can be anything from a buzz to trilling whistles. -
3Systematics and Diversity of Extant Amphibians
Systematics and Diversity of 3 Extant Amphibians he three extant lissamphibian lineages (hereafter amples of classic systematics papers. We present widely referred to by the more common term amphibians) used common names of groups in addition to scientifi c Tare descendants of a common ancestor that lived names, noting also that herpetologists colloquially refer during (or soon after) the Late Carboniferous. Since the to most clades by their scientifi c name (e.g., ranids, am- three lineages diverged, each has evolved unique fea- bystomatids, typhlonectids). tures that defi ne the group; however, salamanders, frogs, A total of 7,303 species of amphibians are recognized and caecelians also share many traits that are evidence and new species—primarily tropical frogs and salaman- of their common ancestry. Two of the most defi nitive of ders—continue to be described. Frogs are far more di- these traits are: verse than salamanders and caecelians combined; more than 6,400 (~88%) of extant amphibian species are frogs, 1. Nearly all amphibians have complex life histories. almost 25% of which have been described in the past Most species undergo metamorphosis from an 15 years. Salamanders comprise more than 660 species, aquatic larva to a terrestrial adult, and even spe- and there are 200 species of caecilians. Amphibian diver- cies that lay terrestrial eggs require moist nest sity is not evenly distributed within families. For example, sites to prevent desiccation. Thus, regardless of more than 65% of extant salamanders are in the family the habitat of the adult, all species of amphibians Plethodontidae, and more than 50% of all frogs are in just are fundamentally tied to water. -
Chemistry and Medical Implications of Novel Amphibian Peptides
Chemistry and Medical lmplications of Novel Amph¡bian Peptides A thesis submitted for the Degree of Doctor of Philosophy by Paul Andrew Wabnitz B.Sc. (Hons) from the Department of Chemistry The University of Adelaide É.ï5 August, 1999 Preface CONTENTS Acknowledgements VI Statement of Originality vil List of Figures vil List of Tables xilt Abstract XV Ghapter l. lntroduction 1.1. Peptide PharmacologY 1.1a.General 1 1.1b. Structure and function of peptide mediators 2 4 1 .1c. Neuropeptides 1.1d. Hormonal pePtides 5 7 1 .1e. Peptide biosYnthesis 1.1f. Peptides as pharmaceuticals I 1.2. Amphibian Peptides 1.2a. Amphibians 10 1.2b. Why studY anurans? 10 1.2c. Anuran antibacterial peptides 16 I .2d. Anuran neuroPePtides 23 1.2e. The Litoria genus 24 1.3. Methodology 1.3a. Surface electrical stimulation 25 1.3b. Analysis bY HPLC 27 1.3c. Mass sPectrometry 28 1.3d. Vacuum Generators ZAB 2HF mass spectrometer 29 1.3e. Finnigan LCQ electrospray mass spectrometer 34 1.3f. Peptide sequencing 38 1.3g. C-terminal end group determination 40 1.3h. Preparation of synthetic peptides 40 41 1.4. References - Preface - Ghapter 2. lnvestigation of Bioactive Peptide Development in the Tadpole Gycle of Litoria splendida 2.1. lntroduction 2.1a. General 46 2.1b. Litoria splendida 50 2.1c. Development cycle of Litoria splendida 54 2.2. Results and Discussion 2.2a. General 56 2.2b. Structure determination 58 2.2c. lsolation of peptides from lawal Litoria splendida 58 2.2d. Conclusions 71 2.3. Experimental 2.3a. Collection and preparation of adult Litoria splendida secretions 73 2.3b. -
Yellow (Bumble Bee) Poison Dart Frog Dendrobates Leucomelas Family
Yellow (Bumble Bee) Poison Dart Frog Dendrobates leucomelas Family: Dendrobatidae Locale: Brazil; Colombia; Guyana; Venezuela Habitat: Found in moist or wet forests in lowlands, found in leaf litter on forest floors or occasionally on trees at temperatures of 26 to 30C. Average Size: Range from 3.1 to 5 cm (1.2 to 1.97in) with average of 4 cm (1.57in) Average Lifespan: 10 to 15 years, but the oldest known lived to 20.5 years Activity: Diurnal Captive care: Rainforest terrarium: a breeding pair or trio can be housed in a 10 gallon well- planted aquarium with good drainage. Expanded clay pellets covered with sphagnum moss and planted with pothos and bromeliads works well. Water should be shallow as these frogs are not strong swimmers and can drown. A small hide, such as half a coconut shell or flowerpot will provide a breeding site. The tank should be covered with a hinged glass lid that has 1.5-2 inches of plastic along back in which ~6 small holes have been drilled (allows air exchange while maintaining high humidity, 95%). A small pump can be used to generate a stream, put pump in one corner (cover with clay pellets) and run aquarium tubing under rock to other corner where rocks are piled to generate a waterfall and rock stream. Temperature: Poison dart frogs prefer moderate temperatures. Daytime temperatures between 75F and 84F (23.5C to 29C). Lighting: Frogs need light to see their food, and full spectrum lighting is needed to maintain the plants. Diet: Insectivores: in captivity, the frogs are fed flightless fruit flies or pinhead crickets daily. -
Poison Dart Frogs and South American Indians
Copyrighted Material 118 F r o G s A N d t o A d s In medieval Europe, frogs and toads were synonymous with evil and witchcraft and toads are depicted as the familiars and alter-egos of witches. Almost all writings of the time portray the toad in a negative fashion (frogs are hardly mentioned) and Shakespeare refers to them as ‘ugly and venomous’. Even today, toads are not welcome in some places and are thought to cause warts, while superstitions concerning toads were commonplace in parts of England and North America until recently. Kenneth Grahame’s The Wind in the Willows, published in 1908, characterizes Toad as pompous and unreliable, but he redresses the balance by also describing him as intelligent and witty. In other children’s tales from Europe, frogs are princes in disguise waiting only for a maiden’s kiss. Poison dart frogs and South American Indians south American Indians of the Emberá Chocó group of frog was enough to kill at least 20,000 mice when tribes from the Pacific slopes of the Andes in Colombia injected under the skin. Extrapolating to humans this is use at least three species of poison dart frogs to tip their equivalent to six or seven average-sized people though blowgun darts: Phyllobates aurotaenia, P. bicolor and comparing toxicity between mice and humans is not P. terribilis. the poison consists of steroidal alkaloids in always completely accurate. the frogs’ skin secretions, of which the most powerful the Indians make their blowguns from a length of is produced by the golden poison dart frog, P.