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(12) United States Patent (10) Patent No.: US 8,911,984 B2 Nakagawa Et Al

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(12) United States Patent (10) Patent No.: US 8,911,984 B2 Nakagawa Et Al USOO891 1984B2 (12) United States Patent (10) Patent No.: US 8,911,984 B2 Nakagawa et al. (45) Date of Patent: Dec. 16, 2014 (54) TANNASE, GENE ENCODING SAME, AND Food Safety Commission, “Safety evaluation standard of additives PROCESS FOR PRODUCING SAME produced in use of genetically modified organisms' determined on Mar. 25, 2004, pp. 1-10. (71) Applicant: Amano Enzyme Inc., Nagoya (JP) Ascención et al., "A novel tannase from Aspergillus niger with (72) Inventors: Megumi Nakagawa, Kakamigahara B-glucosidase activity.” Microbiology, vol. 149, 2003, pp. 2941 2946. (JP): Naoki Matsumoto, Kakamigahara Batra et al., “Potential tannase producers from the genera Aspergillus (JP); Hidoshi Amano, Kakamigahara and Penicillium," Process Biochemistry, vol. 40, 2005, pp. 1553 (JP); Shotaro Yamaguchi, 1557. Kakamigahara (JP) Robert W.-J., “Tannase from Aspergillus awamori: Purification, char (73) Assignee: Amano Enzyme Inc., Nagoya-shi (JP) acter-ization, and the elucidation of the role of tannins in low-ering in vitro protein digestibility of black beans.” Dissertation Abstract. (*) Notice: Subject to any disclaimer, the term of this International B, vol. 50, Bo.7, 1989, p. 2902-2902-B and a cover patent is extended or adjusted under 35 page. U.S.C. 154(b) by 0 days. Sharma et al., “Isolation, purification and properties of tannase from Aspergillus niger van Tieghem.” World Journal of Microbiol. & (21) Appl. No.: 14/088,862 Biotechnol., vol. 15, No. 6, 1999, pp. 673-677 and a cover page. (22) Filed: Nov. 25, 2013 Dhar et al., “Purification, Crystallisation and Physico-Chemical Properties of Tannase of Aspergillus niger," Leather Science, vol. 11, (65) Prior Publication Data No. 2, 1964, pp. 27-38. US 2014/OO93939 A1 Apr. 3, 2014 Pel H.J., “unnamed protein product Aspergillus niger”. GenBank Accession CAK38707.1, EMBL Accession AM270075.1, Mar. 24, 2007, retrieved on Oct. 23, 2009.J. Retrieved from the Internet: Related U.S. Application Data <URL: http://www.ncbi.nlm.nih.gov/protein/134058723> (2 pages). Mahapatra et al., “Purification, characterization and some studies on (62) Division of application No. 13/124.510, filed as Secondary structure of tannase from Aspergillus awamori application No. PCT/JP2009/005105 on Oct. 2, 2009, nakazawa," Process Biochem... vol. 40, 2005, pp. 3251-3254. now Pat. No. 8,617,865. International Search Report dated Nov. 10, 2009, issued for PCT/ (30) Foreign Application Priority Data JP2009/005105. Mahapatra et al., “Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori Oct. 24, 2008 (JP) ................................. 2008-274360 nakazawa," Process Biochemistry, 40, 2005, pp. 3251-3254. (51) Int. Cl. Office Action dated Mar. 12, 2012, issued for the Chinese patent CI2N L/20 (2006.01) application No. 200980 142179.1. CI2N 9/14 (2006.01) An extended European Search Report issued in corresponding EP CI2N IS/00 (2006.01) Patent Application No. 0982 17473.4, dated Jul. 5, 2012. CI2N 9/18 (2006.01) Banerjee et al., “Production and characterization of extracellular and CI2P 7/42 (2006.01) intracellular tannase from newly isolated Aspergillus aculeatus DBF 9.” Journal of Basic Microbiology, vol. 41, Issue 6, pp. 313-318, Dec. (52) U.S. Cl. 2001. CPC. CI2N 9/18 (2013.01): CI2P 7/42 (2013.01) Accession No. A1DD15 (Jan. 23, 2007). USPC ..................... 435/252.3; 435/320.1; 435/195; Kanauchietal. “Purification and Characteristics of Feruloyl Esterase 536/23.2 from Aspergillus awamori G-2 Strain”. Journal of Food Science (58) Field of Classification Search (Aug. 2008): 73(6): C458-C463. CPC ................ C12N 9/14: C12N 9/18: C12P 7/42 Barthomeufetal. "Production, Purification and Characterization of a USPC .................................................. 435/195, 197 Tannase from Aspergillus niger LCF 8.” Journal of Fermentation & See application file for complete search history. Bioengineering (1994), 77(3): 320-323. (56) References Cited Primary Examiner — Tekchand Saidha U.S. PATENT DOCUMENTS (74) Attorney, Agent, or Firm — Edwards Wildman Palmer LLP 2003/0O82595 A1 5/2003 Jiang et al. 2004/0082053 A1 4/2004 Machida et al. (57) ABSTRACT FOREIGN PATENT DOCUMENTS Disclosed is a thermostable tannase derived from a microor CN 10 1260392 A 9, 2008 JP 48-035478 B2 10, 1973 ganism. Specifically disclosed is a thermostable tannase JP 08-080196. A 3, 1996 derived from Aspergillus awamori or Aspergillus niger. A JP 2003-250588 A 9, 2003 preferred embodiment of the tannase has the following WO WO-03/O12071 A2 2/2003 chemoenzymatic properties: (1) activity: to act on a depside OTHER PUBLICATIONS bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) Cristóbal et al., “Microbial tannases: advances and perspectives.” thermal stability: stable at a temperature up to 65°C. (pH 5.0, Appl. Microbiol. Biotechnol., vol. 76, 2007, pp. 47-59. 30 min.). Hatamoto et al., “Cloning and sequencing of the gene encoding tannase and a structural study of the tannase subunit from Aspergillus oryzae,' Gene, vol. 175, 1996, pp. 215-221. 3 Claims, 5 Drawing Sheets U.S. Patent Dec. 16, 2014 Sheet 1 of 5 US 8,911,984 B2 Fig. 1 Optimum temperature 100% 90% 80% 70% 60% 50% 40% 30% 20% 10% O% 3OC 4OC 50°C 6OC 7OC 8OC U.S. Patent Dec. 16, 2014 Sheet 2 of 5 US 8,911,984 B2 Fig. 2 Optimum pH 1 10% 100% 90% 80% 70% 60% 50% 40% 30% 20% 10% 0% 3.0 3.5 40 4.5 5.0 5.5 6.O 65 7.O 75 8.0 pH U.S. Patent Dec. 16, 2014 Sheet 3 of 5 US 8,911,984 B2 Fig. 3 Thermostability 100%90% al g 80% 70% 60% 50% 40%30% 20% 10% O% 30°C 40°C 50°C 60°C 70°C 80°C U.S. Patent Dec. 16, 2014 Sheet 4 of 5 US 8,911,984 B2 Fig. 4 pH stability 90% - -- O 80% Y-/ 70% 60% 50% 40% 30% 20% 10% O% 3.0 3.5 4.0 4.5 5.0 5.5 6.O 6.5 7.O 7.5 8.0 pH U.S. Patent Dec. 16, 2014 Sheet 5 of 5 US 8,911,984 B2 Fig. 5 COR US 8,911,984 B2 1. 2 TANNASE, GENE ENCODING SAME, AND Non-patent Document 2: Gene, Vol. 175, pp. 215-221, 1996 PROCESS FOR PRODUCING SAME Non-patent Document 3: Food Safety Commission “Safety evaluation standard of additives produced in use of geneti TECHNICAL FIELD cally modified organisms' determined on Mar. 25, 2004 The present invention relates to novel tannase produced by Non-patent Document 4: Microbiology, Vol. 149, pp. 2941 a microorganism belonging to the genus Aspergillus. Specifi 2946, 2003 cally, the invention relates to tannase produced by the genus Non-patent Document 5: Process Biochemistry, Vol. 40(5), Aspergillus, which is excellent in thermostability, a gene April 2005, pp. 1553-1557, 2005 encoding the tannase, a process for producing the tannase, a use of the tannase, and the like. 10 SUMMARY OF THE INVENTION BACKGROUND ART Problems to be Solved by the Invention Tannase (tannin acyl hydrolase, EC3.1.1.20) is an enzyme An object of the present invention is to provide tannase that hydrolyzes a depside bond of tannins. In the food indus 15 try, tannase is used for prevention of cream down in tea excellent in thermostability, which is derived from microor beverages, prevention of lee in fruit juice beverages, clarifi ganisms existing in the natural world. Another object of the cation of beer, and the like. invention is also to provide a gene of the tannase, a process for Production of tannase by bacteria, yeasts, and filamentous producing the tannase and a use of the tannase. fungi has been reported in large numbers So far (Non-patent Document 1). There are many reports about the genus Means for Solving the Problems Aspergillus and the genus Penicillium for the filamentous fungi. Chemoenzymatic properties, an amino acid sequence The present inventors have intensively investigated in and a gene encoding tannase have been revealed for tannase order to solve the above-mentioned problems. As a result, derived from Aspergillus Oryzae (see Patent Documents 1 and 25 production of tannase having high thermostability was 2, and Non-patent Document 2). observed in totally 5 strains that are 3 strains of Aspergillus The tannase described in Patent Document 1 has mild acidity Such as an optimum pH range around from 5.0 to 5.5, niger and 2 strains of Aspergillus awamori. Further investi and an optimum temperature range of around 40°C., and is gations proceeded and the present inventors succeeded in deactivated within 10 minutes at a high temperature Such as isolation of tannase produced by Aspergillus awamori higher than 60° C. Therefore, the tannase is reacted under 30 NBRC-4033 (IFO-4033) and determined the chemoenzy very limited conditions in industrial applications. The tannase matic properties thereof. They also succeeded in identifica described in Patent Document 2 is excellent in thermostabil tion of the amino acid sequence of the tannase and a base ity, a residual activity after treatment at 65°C. for 10 minutes sequence of a gene encoding the tannase. As a result of in a citric acid buffer solution (pH 5.5) is 80% or more, and an comparing the chemoenzymatic properties and the amino optimum temperature range is from 60 to 80°C., and thus, the 35 acid sequence to those of tannase previously reported, the defect of the tannase described in Patent Document 1 is over tannase Successfully obtained and identified was confirmed to come, but production of a recombinant was only reported and be novel. production of a non-recombinant is not confirmed.
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