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University of Groningen Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88 Pel, Herman J.; de Winde, Johannes H.; Archer, David B.; Dyer, Paul S.; Hofmann, Gerald; Schaap, Peter J.; Turner, Geoffrey; Albang, Richard; Albermann, Kaj; Andersen, Mikael R. Published in: Nature Biotechnology DOI: 10.1038/nbt1282 IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2007 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Pel, H. J., de Winde, J. H., Archer, D. B., Dyer, P. S., Hofmann, G., Schaap, P. J., Turner, G., Albang, R., Albermann, K., Andersen, M. R., Bendtsen, J. D., Benen, J. A. E., van den Berg, M., Breestraat, S., Caddick, M. X., Contreras, R., Cornell, M., Coutinho, P. M., Danchin, E. G. J., ... de Vries, R. P. (2007). Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nature Biotechnology, 25(2), 221-231. https://doi.org/10.1038/nbt1282 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license. More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne- amendment. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date: 30-09-2021 Supplementary Table 7 A. niger CBS 513.88 gene expression Gene name day3 day3 day3_Detection day5 day5_ day5_Detection Descriptions Signal Detection p-value Signal Detection p-value An01e00180 3.9 A 0.216384 15.7 P 0.04219 trnaKctt An01e00230 42.2 P 0.02493 51.1 P 0.001437 trnaDgtc An01e01100 10 A 0.07897 24.1 P 0.001851 5S ribosomal RNA An01e01100 58.8 P 0.001109 61.1 P 0.001437 trnaPagg An01e01570 14.2 A 0.406973 6.8 A 0.437665 trnaItat An01e01910 4.4 A 0.759912 3.8 A 0.5 trnaAtgc An01e02450 56.6 P 0.017085 65.3 P 0.017085 trnaDgtc An01e02580 38 A 0.085938 61.5 P 0.009766 trnaMcat An01e02710 44.7 P 0.011455 35.7 P 0.02987 transposon Vader - Aspergillus niger An01e03830 14.9 A 0.173261 16.7 A 0.240088 trnaEttc An01e03890 8.8 A 0.376842 17 A 0.265142 trnaEttc An01e04290 4.8 A 0.173261 1.3 A 0.406973 trnaMcat An01e04780 127.9 P 0.001437 84.9 P 0.001109 trnaVcac An01e05430 184.6 P 0.00302 98.6 P 0.001851 trnaVaac An01e05550 3.9 A 0.70854 0.5 A 0.805907 trnaTagt An01e06590 3 A 0.652557 0.6 A 0.95781 trnaFgaa An01e06680 131.4 P 0.001109 86.6 P 0.001109 trnaVcac An01e07610 90.6 P 0.04974 68.1 P 0.02987 trnaLaag An01e07880 62 P 0.000977 55.4 P 0.000977 trnaEctc An01e08320 25.9 A 0.173261 22 A 0.265142 trnaSgct An01e08330 22.9 A 0.194093 15.8 A 0.318935 trnaSgct An01e08340 17.8 A 0.194093 7.4 A 0.623158 trnaSgct An01e08350 19.1 A 0.318935 7.8 A 0.437665 trnaSgct An01e08940 36.6 P 0.014028 30.9 P 0.017085 trnaHgtg An01e09990 9.1 A 0.437665 11.7 A 0.562335 trnaSaga An01e10260 18.4 P 0.04219 18.4 A 0.173261 trnaAtgc An01e11040 115.3 P 0.001109 81.9 P 0.001109 trnaQctg An01e11050 30 A 0.27417 21.8 A 0.19458 trnaQttg An01e11060 39.6 P 0.002371 27.4 P 0.004816 trnaQctg An01e11230 104 P 0.003825 121 P 0.001109 transposon Vader - Aspergillus niger An01e11460 6.9 A 0.531264 3.4 A 0.468736 trnaCgca An01e11490 31.9 M 0.058332 39.1 P 0.02987 trnaSaga An01e11980 99.3 P 0.011455 50.7 P 0.035595 trnaYgta An01e12140 141.4 P 0.001109 117.5 P 0.001109 trnaGgcc An01e12510 36.3 A 0.136048 20.2 A 0.07897 trnaScga An01e12650 41.5 P 0.009301 47.7 P 0.007511 trnaVcac An01e12870 88.2 P 0.004816 70.7 P 0.014028 trnaLaag An01e12900 78 P 0.017085 67.9 P 0.02493 trnaLaag An01e12930 14.4 A 0.29146 13.1 A 0.376842 trnaSaga An01e13800 66.3 P 0.000977 63.3 P 0.000977 trnaEctc An01e15090 5 A 0.468736 2.2 A 0.562335 probable transposon Tndm3 - Aspergillus niger An01g00010 2 A 0.863952 8.1 A 0.652557 hypothetical protein [truncated ORF] An01g00020 23.3 A 0.318935 29.8 A 0.406973 weak similarity to nucleotide binding protein phnN - Escherichia coli An01g00030 394.2 P 0.002371 381.5 P 0.002371 strong similarity to HGH1 - Saccharomyces cerevisiae An01g00040 192.6 P 0.014028 285.4 P 0.006032 strong similarity to alpha subunit of transcription initiation factor TFIIF TFG1 - Saccharomyces cerevisiae [truncated ORF] An01g00050 57.3 M 0.058332 168.7 P 0.004816 similarity to fatty-acyl-CoA synthase beta chain FAS1 - Saccharomyces cerevisiae [truncated orf] An01g00060 776.2 P 0.001109 791.8 P 0.001109 strong similarity to fatty acid synthase, subunit alpha FAS2 - Schizosaccharomyces pombe An01g00070 73.9 P 0.020695 73.5 P 0.006032 strong similarity to N2,N2-dimethylguanosine tRNA methyltransferase trm1 - Schizosaccharomyces pombe An01g00080 98.7 P 0.001437 84.8 P 0.001437 weak similarity to probable membrane protein YOR228c - Saccharomyces cerevisiae An01g00090 35.3 A 0.437665 45.3 A 0.376842 weak similarity to hypothetical protein F21E10.7 - Arabidopsis thaliana An01g00100 950.1 P 0.001109 1046.6 P 0.001109 strong similarity to pyruvate dehydrogenase beta chain precursor PDB1 - Saccharomyces cerevisiae An01g00110 251.5 P 0.011455 272.5 P 0.007511 strong similarity to hypothetical protein SPAC1039.02 - Schizosaccharomyces pombe An01g00120 268.7 P 0.001109 295.1 P 0.001109 similarity to mitochondrial respiratory function protein MRF1 - Saccharomyces cerevisiae An01g00130 83.8 P 0.002371 81.3 P 0.001437 strong similarity to glutamyl-tRNA (Gln) amidotransferase chain A gatA - Bacillus subtilis An01g00140 22.4 A 0.173261 16 A 0.104713 weak similarity to trithorax protein ash2 - Drosophila melanogaster An01g00150 258.9 P 0.001109 288.2 P 0.001437 strong similarity to nuclear protein ENP1 - Saccharomyces cerevisiae An01g00160 1373.5 P 0.001109 1319.3 P 0.001437 similarity to regulator of unfolded protein response (UPR) Hac1p - Saccharomyces cerevisiae An01g00170 223.1 P 0.011455 208.9 P 0.014028 similarity to FTI1 protein - Saccharomyces cerevisiae An01g00190 3.5 A 0.931951 7.8 A 0.941668 similarity to PRIB protein - Lentinus edodes An01g00200 129 P 0.002371 108.7 P 0.002371 strong similarity to succinyl-CoA:3-ketoacid-CoA transferase SCOT - Homo sapiens An01g00210 40.7 A 0.136048 46.6 A 0.136048 hypothetical protein An01g00220 36.8 A 0.29146 62 A 0.136048 strong similarity to hypothetical membrane protein YJR124c - Saccharomyces cerevisiae [truncated] An01g00240 13.6 A 0.759912 22.6 A 0.531264 strong similarity to cytochrome P450 protein 4F5 - rattus norvegicus An01g00250 2.5 A 0.964405 3.2 A 0.95781 strong similarity to nitrate reductase NR2 - Arabidopsis thaliana An01g00260 1.4 A 0.783616 1.5 A 0.805907 strong similarity to hypothetical transcription activator SPAC139.03 - Schizosaccharomyces pombe An01g00270 32.4 A 0.240088 30.2 A 0.194093 similarity to fluconazole resistance protein FLU1 - Candida albicans [putative frameshift] An01g00280 123.6 P 0.003825 226.4 P 0.001437 strong similarity to hypothetical protein PAC823.14 - Schizosaccharomyces pombe An01g00290 102.4 P 0.017085 38.7 A 0.153911 similarity to the hypothetical protein encoded by An17g00430 - Aspergillus niger An01g00310 1.1 A 0.783616 2.5 A 0.805907 strong similarity to the hypothetical protein encoded by An02g10090 - Aspergillus niger An01g00320 11.5 A 0.406973 10.1 A 0.468736 similarity to the hypothetical protein encoded by An08g08380 - Aspergillus niger An01g00330 8 A 0.376842 24.3 A 0.136048 alpha-l-arabinofuranosidase a precursor abfA - Aspergillus niger An01g00340 384.5 P 0.001437 354.3 P 0.001109 strong similarity to hypothetical protein SPCC320.08 - Schizosaccharomyces pombe An01g00360 4.3 A 0.92103 3 A 0.95781 hypothetical protein An01g00370 60.9 A 0.216384 193.2 P 0.011455 strong similarity to aspergillopepsin apnS - Aspergillus phoenicis An01g00380 15.6 A 0.29146 11 A 0.29146 similarity to monocarboxylate transporter MCT3 - Homo sapiens An01g00390 116 P 0.04219 107.6 A 0.068049 hypothetical protein An01g00400 69.6 P 0.04974 67.5 P 0.04219 strong similarity to hypothetical protein SPBC12C2.09c - Schizosaccharomyces pombe An01g00420 36.9 A 0.29146 33.8 A 0.136048 strong similarity to the hypothetical protein encoded by An01g01580 - Aspergillus niger An01g00430 133 P 0.011455 50.6 P 0.04219 similarity to heterokaryon incompatibility protein het-6 - Neurospora crassa An01g00450 5.1 A 0.652557 3.7 A 0.562335 similarity to 3-hydroxyisobutyrate dehydrogenase mmsB - Pseudomonas aeruginosa An01g00460 102.8 P 0.001109 179.3 P 0.001109 hypothetical protein An01g00470 4.9 A 0.468736 19.4 A 0.194093 strong similarity to hypothetical protein jhp0584 - Helicobacter pylori An01g00480 72.5 P 0.001437 24.7 P 0.006032 strong similarity to the hypothetical protein encoded by An08g06260 - Aspergillus niger An01g00490 4.8 A 0.681065 24.7 A 0.562335 strong similarity to isoamyl alcohol oxidase mreA - Aspergillus oryzae An01g00500 21.5 A 0.07897 11 A 0.347443 hypothetical protein An01g00510 29.4 P 0.035595 19.2
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    Biocatalysis and Agricultural Biotechnology 21 (2019) 101342 Contents lists available at ScienceDirect Biocatalysis and Agricultural Biotechnology journal homepage: http://www.elsevier.com/locate/bab Tannin degradation by phytopathogen’s tannase: A Plant’s defense perspective Kanti Prakash Sharma Department of Biosciences, Mody University of Science and Technology, Lakshmangarh, Sikar, Rajasthan, 332311, India ARTICLE INFO ABSTRACT Keywords: Tannins are plant secondary metabolites and characterized as plant defensive molecules. They impose a barrier Tannin against phytopathogens invasion in plants and thus oppose diseases occurrence in them. Tannase is an enzyme Tannase known for its ability to degrade plant tannins. Important plant pathogens also possess tannase coding sequence in Plant defense their genome. Researches on tannase are till date focused on its applications in animal nutrition, in bioreme­ Pathogen diation and in food industries etc. The information about tannase role with respect to pathogen’s virulence is Virulence very scanty or almost nil in scientific literature. The presence of tannase in pathogen’s genome is an adaptive feature which may be a part of its strategy to overcome the negative effects of plants tannins. The present review summarizes important aspects of tannase and its possible role in disease causing ability of pathogens in plants. 1. Introduction hydrolyzable tannins (Zhang et al., 2001). In complex tannins, a cate­ chin or epicatechin unit is bound glycosidically to a gallotannin or an Tannins are universally present in plants and represent the fourth ellagitannin unit to yield catechin or epicatechin and gallic acid or most abundant group of secondary metabolites after cellulose, hemi­ ellagic acid upon hydrolysis.
  • Maleylacetate Reductase from Trichosporon Cutaneum

    Maleylacetate Reductase from Trichosporon Cutaneum

    Biochem. J. (1980) 185, 783-786 783 Printed in Great Britain Maleylacetate Reductase from Trichosporon cutaneum Andras B. GAAL and Halina Y. NEUJAHR Department ofBiochemistry and Biochemical Technology, The Royal Institute of Technology, S 100 44 Stockholm, Sweden (Received 12 November 1979) The enzyme catalysing the reduction of maleylacetate to 3-oxoadipate was purified 150-fold from Trichosporon cutaneum, induced for aromatic metabolism by growth with resorcinol as a major carbon source. The enzyme separated upon electrofocusing into three species with pI values 4.6, 5.1 and 5.6. They had similar catalytic properties and the same molecular weight. Our group has previously reported that the yeast fer, pH 7.6, 0.2pmol of maleylacetate, 0.2,mol of Trichosporon cutaneum can be induced to metabol- NADPH and 50-200#ug of enzyme protein. Under ize phenol and resorcinol, both phenols being these conditions 1 enzyme unit corresponds to an attacked by the same hydroxylating enzyme and the absorbance decrease of 6.1 A340 units/min. same ring-cleavage enzyme (Neujahr & Varga, Determination of molecular weight. Molecular 1970; Varga & Neujahr, 1970). We have since weight was determined by gel filtration (Andrews, demonstrated that both phenol and resorcinol are 1965) on a Sephadex G-150 column metabolized to 3-oxoadipate (Gaal & Neujahr, (2.5cm x 85cm) equilibrated with 50mM-Tris/ 1979). The metabolism of resorcinol goes through H2SO4 buffer, pH 7.6. Alcohol dehydrogenase 1,2,4-trihydroxybenzene and maleylacetate. The (mol.wt. 150000), lactate dehydrogenase (mol.wt. enzyme catalysing the reduction of maleylacetate to 136000), hexokinase (mol.wt. 105000) and avidin 3-oxoadipate was enriched from crude extracts (mol.wt.