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3 rd International Glucosinolate Conference 2014 GLUCOSINOLATES & BEYOND Sponsors rd Proceedings Proceedings | 3 International Glucosinolate Conference 2014 12th October – 15th October 2014 | Wageningen | The Netherlands Guusje Bonnema en Ruud Verkerk ISBN 978-94-6257-382-6 Proceedings of the 3rd International Glucosinolate Conference 2014 “Glucosinolates and Beyond” Wageningen University Editors: Guusje Bonnema Ruud Verkerk 12th October – 15th October 2014 Wageningen The Netherlands Proceedings of the 3rd International Glucosinolate Conference 2014 “Glucosinolates and Beyond” Wageningen University 12th October – 15th October 2014 Conference Centre and Hotel De Wageningsche Berg, Wageningen The Netherlands Editors: Guusje Bonnema and Ruud Verkerk Wageningen University P.O. Box 47, 6700 AA Wageningen, the Netherlands Quotation from or reference to any part of this book should be made with full reference to the ISBN number mentioned below Lay-out: Hedy Wessels Cover design: Wageningen UR, Communication Services ISBN: 978-94-6257-382-6 Printing: MediaCenter Rotterdam BV, The Netherlands 2 Welcome 3 Welcome to the third international glucosinolate conference: Glucosinolates and beyond. Dear colleagues, On behalf of the organizing committee, we have the pleasure to welcome you to Wageningen, The Netherlands, to attend the 3rd international glucosinolate conference “Glucosinolates and Beyond”. This conference is a follow-up of previous conferences held in Jena, Germany (2006) and Elsinore, Denmark (2009). We succeeded in attracting over 100 participants, from 23 countries in Europe, Asia, USA and Africa, and sponsors from Dutch graduate schools, research organizations and companies. The mission of Wageningen University and Research Center is: “to explore the potential of nature to improve the quality of life”. Wageningen UR combines the strengths of research institutes and the university in the fields of plant science, agrotechnology and food science, environmental science, animal science and the social sciences. These different disciplines, and the interactions and synergy between disciplines, are also reflected in the breadth of research focused on glucosinolates. During this conference we aim at bringing together scientists that are active in diverse areas of glucosinolate research in order to exchange scientific knowledge and ideas. To stimulate exchange between the disciplines molecular genetics, evolution and ecology, food science, agronomy and human nutrition, we chose four plenary sessions and invited keynote speakers in the respective disciplines and poster sessions introduced by poster flash presentations. This, together with the excursion Tuesday afternoon, where we will take you on a tour of the Arboretum, the city of Wageningen or the Wageningen University campus, followed by a dinner in restaurant O Mundo, in the historical building “Hotel de Wereld”, should lead to new friendships and collaborations between scientists from different disciplines, to strengthen world-wide glucosinolate research. We wish you a very inspiring conference and a pleasant stay in Wageningen. Guusje Bonnema & Ruud Verkerk on behalf of the Scientific Organizing Committee 4 Contents Welcome ........................................................................................................................ 3 Sponsors ........................................................................................................................ 6 Organizing committee .................................................................................................... 7 Conference secretariat ................................................................................................... 7 General Information ....................................................................................................... 9 Program ....................................................................................................................... 11 Oral presentations ........................................................................................................ 17 Opening Session ........................................................................................................... 19 Session 1. Molecular and genetic aspects of glucosinolates .......................................... 23 Session 2. Applications of glucosinolates in agro- and food systems ............................ 33 Session 3. Glucosinolates and human health ................................................................ 43 Session 4. Ecology and evolution of glucosinolates ...................................................... 53 Poster presentations abstracts in alphabetic order ...................................................... 65 Author index .............................................................................................................. 129 5 Sponsors Sponsors 6 Organizing committee Organizing committee Dr Ruud Verkerk Dr Guusje Bonnema Wageningen University, Wageningen UR, Food Quality and Plant Breeding, Design, The The Netherlands Netherlands Dr Tom de Jong Dr Arjen Biere Plant Ecology & Terrestrial Ecology, Phytochemistry, Institute Netherlands Institute of Biology Leiden, for Ecology (NIOO- Leiden University, KNAW) Wageningen, The Netherlands The Netherlands Prof Dr Eric Schranz Prof Dr Nicole van Dam Wageningen University, iDiv Halle-Jena-Leipzig & Biosystematics, RU Nijmegen The Netherlands Conference secretariat Hedy Wessels Wageningen UR, Communication Services, The Netherlands 7 8 General Information Abstracts These Proceedings contain abstracts of keynote speakers, followed by abstracts of oral presentations, and finally poster abstracts. All posters are arranged in alphabetical order of the presenting author. The organising committee does not take any responsibility for scientific of typographical errors. Oral presentations All presentations are plenary and will be held in the Bosrand room, Hotel and Conference Centre De Wageningsche Berg Posters All posters are enlisted in alphabetical order in this book. During the whole conference they will be on display and can be found in the Arboretum room. To enlarge the attention of all participants, poster flash presentations are included in the program. Presenters can briefly highlight their work on one single slide in a plenary session. Excursions and Conference dinner Tuesday 14th October 15.30-22.30 hrs For attending an excursion please subscribe at the registration desk on site 1. Tour Wageningen Campus During this visit you have the opportunity to have a quick glance around Wageningen Campus, where expertise and talent come together. Scientists and students carry out globally pioneering research here or train to become professionals who will make breakthroughs in knowledge and technology in the future. Students will guide you around Wageningen Campus. Transportation by bus between Conference Centre de Wageningsche Berg and Wageningen Campus is arranged. Date and Time: Tuesday 14th October 15.30 – 17.30 hrs, Wageningen 2. Tour Wageningen Centre During this visit you have the opportunity to have a quick glance around the old Centre of Wageningen. There are traces of an early medieval settlement on the mountain (Wageningsche Berg). Presumably people first settled in the foothills of the Western-Veluwe massif in the 12th century. The defence works were modernized early in the 17th century, when the double moat became one single and wider moat. The walls were strengthened by martial ramparts in six places. Towards the end of the 17th century members of the influential Torck family were responsible for numerous changes in and around the castle. Lubbert Adolf Torck (1687-1758), mayor of Wageningen and squire of Rosendael, also ordered the construction of three major (and luxurious) buildings with apartments for rent. Two of these still exist, the most important one being the former administrative centre of the Wageningen University. It now forms the nucleus of the prestigious new housing development built by the well-known Belgian architect Charles Vandenhove. The excursion will start and end at Conference Centre De Wageningsche Berg and will be on foot. To reach Wageningen Centre starting from Hotel de Wageningsche Berg, we will first walk through the botenical garden “Arboretum Bel Monte”. This botanical garden manages special collections of the genera Rhodendron and the Rosaceae, with most important representatives the roses, flowering cherries, ornamental apples, - pears, hawthorns and rowans. Date and Time: Tuesday 14th October 15.30 – 17.30 hrs, Wageningen 9 3. Conference Dinner Hotel De Wereld All participants are invited to join the conference dinner. A great opportunity to network and socialize with other participants while enjoying a delicious dinner. Hotel de Wereld in Wageningen was the site of the capitulation of the German troops in the Netherlands on 5 and 6 May 1945, and the end of German occupation during World War II. On 6 May 1945, the German general Blaskowitz surrendered to the Canadian General Charles Foulkes, which ended the Second World War in the Netherlands. Prince Bernhard, acting as commander-in-chief of the Dutch Interior Forces, attended the meeting as well. The Generals negotiated the terms of surrender in Hotel de Wereld. This fact is remembered annually by a Remembrance ceremony May the 4th and a Liberation festival May the 5th. Transportation by bus between Conference Centre de Wageningsche Berg and Hotel De Wereld is arranged. Date and Time: Tuesday 14th