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Stapled Peptides—A Useful Improvement for Peptide-Based Drugs
molecules Review Stapled Peptides—A Useful Improvement for Peptide-Based Drugs Mattia Moiola, Misal G. Memeo and Paolo Quadrelli * Department of Chemistry, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy; [email protected] (M.M.); [email protected] (M.G.M.) * Correspondence: [email protected]; Tel.: +39-0382-987315 Received: 30 July 2019; Accepted: 1 October 2019; Published: 10 October 2019 Abstract: Peptide-based drugs, despite being relegated as niche pharmaceuticals for years, are now capturing more and more attention from the scientific community. The main problem for these kinds of pharmacological compounds was the low degree of cellular uptake, which relegates the application of peptide-drugs to extracellular targets. In recent years, many new techniques have been developed in order to bypass the intrinsic problem of this kind of pharmaceuticals. One of these features is the use of stapled peptides. Stapled peptides consist of peptide chains that bring an external brace that force the peptide structure into an a-helical one. The cross-link is obtained by the linkage of the side chains of opportune-modified amino acids posed at the right distance inside the peptide chain. In this account, we report the main stapling methodologies currently employed or under development and the synthetic pathways involved in the amino acid modifications. Moreover, we report the results of two comparative studies upon different kinds of stapled-peptides, evaluating the properties given from each typology of staple to the target peptide and discussing the best choices for the use of this feature in peptide-drug synthesis. Keywords: stapled peptide; structurally constrained peptide; cellular uptake; helicity; peptide drugs 1. -
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GCCL TRAVEL PLANNING GUIDE Grand European Cruise 2022 Learn how to personalize your experience on this vacation Grand Circle Cruise Line® The Leader in River Cruising Worldwide 1 Grand Circle Cruise Line ® 347 Congress Street, Boston, MA 02210 Dear Traveler, At last, the world is opening up again for curious travel lovers like you and me. Soon, you’ll once again be discovering the places you’ve dreamed of. In the meantime, the enclosed Grand Circle Cruise Line Travel Planning Guide should help you keep those dreams vividly alive. Before you start dreaming, please let me reassure you that your health and safety is our number one priority. As such, we’re requiring that all Grand Circle Cruise Line travelers, ship crew, Program Directors, and coach drivers must be fully vaccinated against COVID-19 at least 14 days prior to departure. Our new, updated health and safety protocols are described inside. The journey you’ve expressed interest in, Grand European Cruise River Cruise Tour, will be an excellent way to resume your discoveries. It takes you into the true heart of Europe, thanks to our groups of 38-45 travelers. Plus, our European Program Director will reveal their country’s secret treasures as only an insider can. You can also rely on the seasoned team at our regional office in Bratislava, who are ready to help 24/7 in case any unexpected circumstances arise. Throughout your explorations, you’ll meet local people and gain an intimate understanding of the regional culture. Enter the home of a local family in Wertheim for a Home-Hosted Kaffeeklatsch where you will share coffee and cake, and experience what life is like for a typical German family; and chat with a member of Serbia’s Roma community to gain insight into the stigma facing this culture in Europe—and how they are paving the way for a new future for their people. -
Food Consumption Impact Datasets – Questions and Answers
v Food Consumption Impact Datasets – Questions and Answers Farm-to-Fork & Farm-to-Retail Datasets DATE: 19-6-2020 Blonk Consultants helps companies, governments and civil society organisations put sustainability into practice. Our team of dedicated consultants works closely with our clients to deliver clear and practical advice based on sound, independent research. To ensure optimal outcomes we take an integrated approach that encompasses the whole production chain. Title Food Consumption Impact Datasets – Questions and Answers Date 19-6-2020 Place Gouda, NL Authors Mike van Paassen Blonk Consultants Roline Broekema Blonk Consultants Laura Wolf Blonk Consultants Blonk Consultants (+31) 0182 579970 Gravin Beatrixstraat 34 www.blonkconsultants.nl 2805 PJ Gouda [email protected] Contents The Food Consumption Impact Datasets – Q&A..................................................................................................... 2 1. What are the Food Consumption Impact Datasets and what can I use them for? ........................................ 2 How am I allowed to use the datasets? ......................................................................................................... 2 2. In which formats are the datasets available? ................................................................................................. 3 3. What are the differences between the Farm-to-Fork and Farm-to-Retail datasets? .................................... 3 System Boundaries ........................................................................................................................................ -
Plant Proteomics As a Tool for Elucidating the Molecular Mechanisms Underlying Signaling and Plant Interactions with the Environment
PALACKÝ UNIVERESITY OLOMOUC Faculty of Science Plant proteomics as a tool for elucidating the molecular mechanisms underlying signaling and plant interactions with the environment Mgr. Martin Černý, Ph.D. MENDEL UNIVERSITY IN BRNO Faculty of AgriSciences Department of Molecular Biology and Radiobiology HABILITATION THESIS Study field: Biochemistry Brno 2021 Děkuji všem, kteří se přímo i nepřímo zasloužili o tuto práci. Mé rodině a přátelům za podporu a trpělivost. Těm, kteří se mnou spolupracovali a těm, co ve spolupráci pokračovali a pokračují i přes řadu experimentů, které nevedly k očekávaným cílům. V neposlední řadě patří poděkování prof. Břetislavu Brzobohatému, bez kterého by tato práce nevznikla. Contents 1. Introduction ....................................................................................................................... 1 2. Fractionation techniques to increase plant proteome coverage ......................................... 4 2.1. Tissue separation ........................................................................................................ 5 2.2. Subcellular plant proteome ......................................................................................... 7 2.3. Separations at the protein level ................................................................................... 9 2.4. Separation at the peptide level .................................................................................. 11 2.5. Comparison of fractionation techniques .................................................................. -
An Investigation Into the Availability of Horsemeat in Belgium, France and the Netherlands
An investigation into the availability of horsemeat in Belgium, France and the Netherlands October 2012 An investigation into the availability of horsemeat in Belgium, France and the Netherlands Introduction Each year, several hundred thousand tonnes of horsemeat are imported to the European Union, primarily from Canada, Mexico, Brazil, Argentina and Uruguay. This is in addition to the tens of thousands of horses that are slaughtered for human consumption within the EU itself. To gain greater insight into the European horsemeat market, Humane Society International (HSI) conducted a retail investigation in three EU Member States where horsemeat is commonly eaten, namely Belgium, France and the Netherlands. The fieldwork was conducted in June 2012. The aim of this study was to determine the availability and range of horsemeat and products containing horsemeat sold in these countries. It also sought to ascertain whether and what proportion of horsemeat products are labelled with information concerning the meat’s country of origin. Data concerning the price, weight and composition of the horsemeat products were also collected. Horsemeat production and trade in Belgium, France and the Netherlands The retail and consumption of horsemeat in Belgium, France and the Netherlands must be situated in the broader context of these three EU Member States’ involvement in the global horsemeat trade, both in terms of their role as major importers of horsemeat as well as the producers thereof. Each year, several thousand horses are slaughtered domestically in Belgium, France and the Netherlands. Not all of these animals have been necessarily raised in the country where they meet their end. -
UNIVERSITY of CALIFORNIA, SAN DIEGO Biochemical Kinds And
UNIVERSITY OF CALIFORNIA, SAN DIEGO Biochemical Kinds and Selective Naturalism A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Philosophy (Science Studies) by Joyce Catherine Havstad Committee in charge: Professor William Bechtel, Chair Professor Craig Callender Professor Nancy Cartwright Professor Cathy Gere Professor James Griesemer 2014 © Joyce Catherine Havstad, 2014 All rights reserved. The Dissertation of Joyce Catherine Havstad is approved, and it is acceptable in quality and form for publication on microfilm and electronically: __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ Chair University of California, San Diego 2014 iii DEDICATION for my parents iv TABLE OF CONTENTS Signature Page…………………………………………………………………….. iii Dedication…………………………………………………………………………. iv Table of Contents………………………………………………………………….. v List of Figures……………………………………………………………………... viii Vita………………………………………………………………………………… x Abstract of the Dissertation………………………………………………………... xi Introduction………………………………………………………………………... 1 References……………………………………………………………………... 7 Chapter 1: Messy Chemical Kinds………………………………………………… 8 Section 1: Introduction………………………………………………………… 8 Section 2: Microstructuralism about Chemical Kinds…………………………. -
Peelbergen Menu Not Applicable During CSI Shows
Peelbergen menu Not applicable during CSI shows COLD DISHES CHEESE SANDWICH 2,0 HEALTHY SANDWICH/BROODJE GEZOND 3,5 Ham, cheese, egg, tomato, cucumber and mixed lettuce Ham, kaas, ei, tomaat, komkommer en gemengde sla EGG SALAD SANDWICH 2,5 Homemade egg salad and mixed lettuce Huisgemaakte eiersalade en gemengde sla PULLED CHICKEN SANDWICH 3,5 Pulled chicken, mixed lettuce and spicy sauce Kip, gemengde sla en pittige saus ITALIAN CARPACCIO SANDWICH 5,0 Carpaccio, sun dried tomatoes, Parmesan cheese, pine nuts & truffle mayonnaise Carpaccio, zongedroogde tomaten, Parmezaanse kaas, pijnboompitten & truffelmayonaise SMOKED SALMON CIABATTA 5,0 Smoked salmon, cream cheese, onion, capers, honey mustard dressing Gerookte zalm, roomkaas, uien, kappertjes en honingmosterddressing SALADS Salads are served with bread & butter on the side CAESAR SALAD 6,5 Chicken fillet, tomatoes, cucumber, egg, croutons, Parmesan cheese and dressing Kipfilet, tomaat, komkommer, ei, croutons, Parmezaanse kaas en dressing SCAMPI SALAD 6,5 Salad with scampi with lemon mayonnaise, croutons and herbal vinaigrette Salade scampi met citroenmayonaise, croutons en kruidenvinaigrette BRIE CHEESE SALAD 6,5 Salad with brie cheese, pecan, raisins, croutons and sweet apple dressing Salade met brie, pecannoten, rozijnen, croutons en appelstroop dressing Page 1 of 3 WARM Dishes BURLY BURGER 7,0 Beef burger, cheddar cheese, bacon, pickles, tomato salsa, sesame bun Rundvlees hamburger, cheddarkaas, bacon, augurk, tomatensalsa, sesambol NOT A WIENER, BUT A SCHNITZEL 6,0 Chicken schnitzel -
TC İSTANBUL Üniversitesi ( DOKTORA Tezi )
T.C. İSTANBUL ÜNiVERSiTESi SAGLIK BiLiMLERİ ENSTİTÜSÜ ( DOKTORA TEZi ) 1933 ÜNİVERSİTE REFORMU İLE BİRLİKTE TÜRKİYE'YE GELEN YABANCI BİYOKİMYA HOCALARı VE KATKILARI i -- -- --- ŞÜKRÜARAS DANIŞMAN DOÇ. DR. GÜL TEN DİNÇ TIP TARİHİ VE ETİK ANABiLiM DALI DEONTOLOJİ VE TIP TARİHİ PROGRAMI İSTANBUL-2012 ii TEZ ONAYI Aşağıda tanıtımı yapılan tez. jüri tarafından başarılı bulunarak Doktora Tezi olarak kabul edilmiştir. d { n vw 13/06 /2012 Prof. Dr. Nevin Yalınan Enstitü Müdürü)'- Kurum : İstanbul Üniversitesi Sağlık Bilimleri Enstitüsü Program Adı : Deontoloji ve Tıp Tarihi Doktora Programı, Programın Seviyesi: Yüksek Lisans () Doktora ( X ) Anabilim Dalı :Tıp Tarihi ve Etik Tez Sahibi : Şükrü Aras Tez Başlığı : l 933 Üniversite Reformu ile birlikte Türkiye'ye gelen yabancı biyokimya hocaları ve katkı ları Sınav Yeri : İ.Ü. Cerrahpaşa Tıp Fakültesi. Tıp Tarihi ve Etik Anabilim Dalı Sınav Tarihi : l 3.06.20 l 2 Tez Sınav Jürisi \. Doç. Dr. Gülten Dinç (Tez Danışmanı), İstanb~l Üniversitesi. Cerrahpaşa Tıp Fakültesi. Tıp Tarihi ve Etik Anabilim Dalı. j·.J ~~ \ 2. Prof. Dr. Nuran Yıldırım (Tez İzleme Komitesi Üyesi), İstanbul Üniversitesi. İstanbul Tıp Fakültesi. Tıp Tarihi ve Etik Anabilim Dalı. ,X._LGUı \ 3. Prof. Dr. Emre Dölen (Tez İzleme Komitesi Üyesi), Marmara Üniversitesi. Eczacılık Fakültesi. ali ik Kimya Anabilim Dalı. yten Altıntaş, İstanbul Üniversitesi. Cerrahpaşa Tıp Fakültesi. Tıp Tarihi ve Etik Anabilim Dalı. ~-- ~~--j -- 'j .. .. S. Doç. Dr. Yeşim lşıl Ulman. Acıbadem Universitesi. Tıp Fakültesi. Tıp Tarihi ve Etik Anabili~alı. iii BEYAN Bu tez çalışmasının kendi çalışınam olduğunu, tezin planlanmasından yazımına kadar bütün safhalarda etik dışı davranışıının olmadığını, bu tezdeki bütün bilgileri akademik ve etik kurallar içinde elde ettiğimi, bu tez çalışmayla elde edilmeyen bütün bilgi ve yorumlara kaynak gösterdiğimi ve bu kaynakları da kaynaklar listesine aldığımı, yine bu tezin çalışılması ve yazımı sırasında patent ve telif hakiarım ihlal edici bir davranışıının olmadığı beyan ederim. -
Read Travel Planning Guide
GCCL TRAVEL PLANNING GUIDE Romance of the Rhine & Mosel 2021 Learn how to personalize your experience on this vacation Grand Circle Cruise Line® The Leader in River Cruising Worldwide 1 Grand Circle Cruise Line ® 347 Congress Street, Boston, MA 02210 Dear Traveler, At last, the world is opening up again for curious travel lovers like you and me. Soon, you’ll once again be discovering the places you’ve dreamed of. In the meantime, the enclosed Grand Circle Cruise Line Travel Planning Guide should help you keep those dreams vividly alive. Before you start dreaming, please let me reassure you that your health and safety is our number one priority. As such, we’re requiring that all Grand Circle Cruise Line travelers, ship crew, Program Directors, and coach drivers must be fully vaccinated against COVID-19 at least 14 days prior to departure. Our new, updated health and safety protocols are described inside. The journey you’ve expressed interest in, Romance of the Rhine & Mosel River Cruise, will be an excellent way to resume your discoveries. It takes you into the true heart of Europe, thanks to our groups of 38-45 travelers. Plus, our European Program Director will reveal their country’s secret treasures as only an insider can. You can also rely on the seasoned team at our regional office in Bratislava, who are ready to help 24/7 in case any unexpected circumstances arise. Throughout your explorations, you’ll meet local people and gain an intimate understanding of the regional culture. Get a glimpse of everyday German life when you join a family for a Home-Hosted Visit in Speyer, enjoying an afternoon of homemade treats and friendly conversation; and learn about the love and pride poured into Germany’s fine vintages during a behind-the-scenes tour of a winery in Bernkastel, followed by a tasting. -
Fire Brigade Trains at Margraten Fire-Fighters’ Goals Are to Save Lives, Property and the Environment
Volume 25, No. 10 NATO Air Base Geilenkirchen 29 May 2009 Fire Brigade trains at Margraten Fire-fighters’ goals are to save lives, property and the environment Text and photos Control Tower. After he has assigned poisonous gases, and obscures vision. firemen have learned in their initial SMSgt. Johan Hijmenberg his men to their mandatory daily As the interior of an aircraft is a long fire-fighter training. “Compressed air duties on base he drives with a group corridor containing many passengers situational awareness is mandatory The first step of fire-fighting of ten to Margraten, which is located the main task is to create a survivable in the basic training,” said Bours. “If operations is reconnaissance to approximately 50 kilometres from environment, which is done by you don’t succeed in maintaining that search for the origin of the fire, the base. “For the last 15 years we ventilating the area. “Unlike aircraft, awareness you will never be a fire- which may not be obvious for an have used this facility,” said Bours. buildings have many different rooms fighter.” indoor fire, especially when there “Eight times a year we hire it for and spaces,” said Bours. “The dangers are no witnesses, and also to spot a morning session so that all the are quite different from what you The training facility exists of two the specific risks and the possible Fire Brigade personnel receive their can expect when you fight a fire on connected houses: number 77, three casualties. That’s why training is compressed air and hot situational an aircraft. -
De Geschiedenis Van De Scheikunde in Nederland. Deel 1
De geschiedenis van de scheikunde in Nederland. Deel 1 Van alchemie tot chemie en chemische industrie rond 1900 H.A.M. Snelders bron H.A.M. Snelders, De geschiedenis van de scheikunde in Nederland. Deel 1: Van alchemie tot chemie en chemische industrie rond 1900. Delftse Universitaire Pers, Delft 1993 Zie voor verantwoording: http://www.dbnl.org/tekst/snel016gesc01_01/colofon.htm © 2008 dbnl / H.A.M. Snelders II ‘De scheider’, van Jan Luyken (Het menselijk bedrijf Amsterdam, 1694) H.A.M. Snelders, De geschiedenis van de scheikunde in Nederland. Deel 1 vii Ten geleide We schrijven 1992. In onze complexe samenleving staat de scheikunde er niet goed op. ‘Het vak scheikunde is uit de gratie’ schrijft een bekende journalist. ‘Om eerlijk te zijn heb ik in het verleden wel eens verteld dat ik Frans studeerde’ zegt een scheikundig student, om vragen te voorkomen. In 1993 bestaat de Koninklijke Nederlandse Chemische Vereniging, KNCV, negentig jaar. Er zijn in Nederland sinds het vak tot ontwikkeling kwam zeer belangrijke prestaties op scheikundig gebied verricht. Er zijn perioden geweest waarin er voor deze prestaties een brede waardering bestond, dit was misschien het sterkst het geval in de jaren vijftig-zestig van onze eeuw. In de tegenwoordige tijd bestaat ten aanzien van de scheikunde en al het chemisch handelen veelal een sfeer van wantrouwen. In brede kring wordt gedacht dat de scheikunde, de scheikundigen, veroorzakers van, vooral milieugerelateerde, problemen zijn. Er wordt daarbij vergeten dat dezelfde mensen die ervan worden verdacht de veroorzakers van deze problemen te zijn in hoge mate er toe bijdragen dat (milieu)problemen kunnen worden voorkómen, opgespoord en opgelost. -
RIVM Rapport 350070001 Dutch National Food
Dutch National Food Consumption Survey – Young Children 2005/2006 Children Young – Survey Consumption Food National Dutch Report 350070001/2007 M.C. Ocké | C.T.M. van Rossum | H.P. Fransen et al Dutch National Food RIVM National Institute fot Public Health and the Environment Consumption Survey – Centre for Nutrition and Food PO Box 1 Young Children 2005/2006 3720 BA Bilthoven The Netherlands www.rivm.nl RIVM Report 350070001/2008 Dutch National Food Consumption Survey- Young Children 2005/2006 M.C. Ocké C.T.M. van Rossum H.P. Fransen E.J.M. Buurma E.J. de Boer H.A.M. Brants E.M. Niekerk J.D. van der Laan J.J.M.M. Drijvers Z. Ghameshlou Contact: C.T.M. van Rossum Centre for Nutrition and Health [email protected] This investigation has been performed by order and for the account of Ministry of Health, Welfare and Sport, within the framework of 5.4.10 'Food consumption survey -Young Children' RIVM, P.O. Box 1, 3720 BA Bilthoven, the Netherlands Tel +31 30 274 91 11 www.rivm.nl © RIVM 2008 Parts of this publication may be reproduced, provided acknowledgement is given to the 'National Institute for Public Health and the Environment', along with the title and year of publication. 2 RIVM Report 350070001 Abstract Dutch National Food Consumption Survey-Young Children 2005/2006 Young children in the Netherlands aged 2 to 6 years do not consume sufficient amounts of vegetables, fruit, fish and fibre. In addition the intake of saturated fatty acids is higher than recommended in the 4 to 6 year-old age group.