DOCSLIB.ORG

DNA Barcoding Identifies Cryptic Animal Tool Materials

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
DNA Barcoding Identifies Cryptic Animal Tool Materials DNA barcoding identifies cryptic animal tool materials Matthew Steele, Linda Neaves, Barbara Klump, James St Clair, Joana Fernandes, Vanessa Hequet, Phil Shaw, Peter Hollingsworth, Christian Rutz To cite this version: Matthew Steele, Linda Neaves, Barbara Klump, James St Clair, Joana Fernandes, et al.. DNA barcoding identifies cryptic animal tool materials. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2021, 118 (29), pp.e2020699118. hal-03285541 HAL Id: hal-03285541 https://hal.inrae.fr/hal-03285541 Submitted on 13 Jul 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License DNA barcoding identifies cryptic animal tool materials BRIEF REPORT Matthew P. Steelea,1, Linda E. Neavesb,c,1, Barbara C. Klumpa,2, James J. H. St Claira, Joana R. S. M. Fernandesa, Vanessa Hequetd, Phil Shawa, Peter M. Hollingsworthb, and Christian Rutza,3 aCentre for Biological Diversity, School of Biology, University of St Andrews, St Andrews KY16 9TH, United Kingdom; bRoyal Botanic Garden Edinburgh, Edinburgh EH3 5LR, United Kingdom; cThe Fenner School of Environment and Society, The Australian National University, Canberra, ACT 2600, Australia; and dInstitut de Recherche pour le Développement, Centre de Nouméa, 98848 Nouméa, New Caledonia, France Edited by Scott V. Edwards, Harvard University, Cambridge, MA, and approved May 2, 2021 (received for review February 11, 2021) Some animals fashion tools or constructions out of plant materials that three study populations target different species despite living to aid foraging, reproduction, self-maintenance, or protection. just a few kilometers apart (12). While we managed to identify raw Their choice of raw materials can affect the structure and proper- materials at two sites (site-1 and site-2), we failed at the third (site- ties of the resulting artifacts, with considerable fitness conse- 3), even after employing a wide range of well-established field quences. Documenting animals’ material preferences is challenging, methods aimed at observing tool manufacture directly (Fig. 1 B however, as manufacture behavior is often difficult to observe di- and C and SI Appendix). rectly, and materials may be processed so heavily that they lack Given the importance of identifying the crows’ preferred plant identifying features. Here, we use DNA barcoding to identify, from species at site-3 for our overall research program, we tried an just a few recovered tool specimens, the plant species New Caledo- innovative artifact-centered approach (Fig. 1D). We extracted nian crows (Corvus moneduloides) use for crafting elaborate hooked DNA from seven hooked stick tools recovered at the site during stick tools in one of our long-term study populations. The method 2016–2017 and amplified two DNA barcoding regions: trnL-UAA succeeded where extensive fieldwork using an array of conventional [∼500 bp (13)] and internal transcribed spacer (ITS) [∼600 bp approaches—including targeted observations, camera traps, radio- (14)]. All samples produced identical haplotypes. Comparison tracking, bird-mounted video cameras, and behavioral experiments against the National Center for Biotechnology Information with wild and temporarily captive subjects—had failed. We believe (NCBI) Nucleotide nonredundant database indicated the tool that DNA barcoding will prove useful for investigating many other samples belonged to the family Sapotaceae, most likely the genus tool and construction behaviors, helping to unlock significant re- Mimusops or Manilkara (trnL: >98% identity; ITS: >95% iden- search potential across a wide range of study systems. tity). The ITS region exhibited greater resolution and indicated ECOLOGY Mimusops elengi as a candidate (96 to 99% identity). With a animal construction behavior | DNA barcoding | nest building | New putative source identified, we collected reference leaf samples of Caledonian crow | tool use M. elengi and Planchonella cinerea, the only closely related spe- cies known to occur locally, and analyzed them using the same here is increasing interest in the plant materials selected by method. The tool samples and M. elengi reference samples Tnonhuman animals to manufacture foraging tools and con- produced identical haplotypes for both DNA barcodes. Fur- structions (1, 2). Animals’ raw-material preferences can affect thermore, the maximum likelihood phylogenetic tree for ITS the structural and functional properties of artifacts, and, in some produced well-supported lineages that clustered tool samples cases, appear to be socially transmitted, contributing to rudimen- and M. elengi reference samples together, within a wider clade of tary material “cultures” (3–5). Two complementary approaches are non-New Caledonian M. elengi sequences (Fig. 1 D, iii). P. cinerea available for identifying plant materials used by wild animals: direct reference samples clustered within the Sapotaceae, but outside the observation of manufacture behavior (“animal-centered”) and ex- Mimusops genus, as expected, confirming that the crow tools from amination of artifacts in isolation from the behavior that created site-3 were made from M. elengi. We subsequently verified that them (“artifact-centered”). The latter, adopted by necessity in ar- wild-caught, temporarily captive New Caledonian crows readily chaeology, is particularly useful when animals cannot be habituated manufacture hooked stick tools from this material (this work was or are otherwise difficult to observe, but can present considerable conducted at site-1, since birds from site-3 proved too difficult to challenges. Artifacts are often heavily processed (lacking features work with in field aviaries). that aid identification, such as leaves or flowers), may be physically The use of DNA barcoding has led to an important break- distanced from the raw materials from which they were produced through for our research program. Reliable raw-material iden- (because the animal transported them), and may comprise a tification is key to uncovering the drivers of the striking regional complex assemblage of materials from different sources (such as in divergence we observed in an important aspect of New Caledo- bird nests). In these cases, material identification has so far relied nian crows’ hooked stick tool-making behavior (15). Specifically, on expert knowledge, which may be difficult and expensive to ac- with a set of three study populations established, and the ability — quire (6). Here, we demonstrate that DNA barcoding the use of to conduct rapid surveys across additional replicate sites, it will standardized DNA regions to identify organic material to species level (7)—provides a robust, cost- and time-efficient solution to these problems. Author contributions: M.P.S., L.E.N., B.C.K., J.J.H.S.C., P.M.H., and C.R. designed research; New Caledonian crows (Corvus moneduloides) are renowned M.P.S., L.E.N., B.C.K., J.J.H.S.C., J.R.S.M.F., V.H., P.S., P.M.H., and C.R. performed research; for their ability to manufacture complex foraging tools (8). When M.P.S., L.E.N., B.C.K., and P.M.H. analyzed data; M.P.S., L.E.N., and C.R. wrote the paper; making a hooked stick tool, they select a forked plant stem, and C.R. led, and secured funding for, the long-term project. remove a suitable branch, trim off any leaves and twiglets, and The authors declare no competing interest. often refine the tool by sculpting the remains of the nodal joint This open access article is distributed under Creative Commons Attribution License 4.0 into a neat terminal hook, stripping bark near the functional end, (CC BY). and bending the tool shaft (9). These processing steps substan- 1M.P.S. and L.E.N. contributed equally to this work. tially alter the appearance of the plant material (Fig. 1A). Im- 2Present address: Max Planck Institute of Animal Behavior, 78315 Radolfzell, Germany. portantly, properties of the raw material affect the morphology 3To whom correspondence may be addressed. Email: [email protected]. of the resulting tools, which in turn affects foraging efficiency This article contains supporting information online at https://www.pnas.org/lookup/suppl/ (9–11). New Caledonian crows are highly selective when choosing doi:10.1073/pnas.2020699118/-/DCSupplemental. plants for hooked stick tool manufacture: we recently discovered Published July 12, 2021. PNAS 2021 Vol. 118 No. 29 e2020699118 https://doi.org/10.1073/pnas.2020699118 | 1of3 Downloaded by guest on July 12, 2021 AB Site-1: Mixed dry forest Tool material: mixed Site-2: Farmland Tool material: Desmanthus virgatus Site-3: Coastal/residential Tool material: Mimusops elengi (this study) 1 km C Initial investigations Research focus on site-2 Aviary experiments Raw material of NC crow tools at site-3 is shown Work focuses on site-2 where wild and Temporarily captive NC crows from site-3 to differ from that at site-2, but field observations, temporarily captive NC crows make are presented with candidate plant species aviary experiments and camera traps fail to hooked stick tools from the non-native M. elengi (along with other locally sourced identify the plant species. perennial shrub D. virgatus. plants) – low task engagement. Bird-mounted video loggersField experiments VHF radio-tracking Miniature cameras are attached to NC A variety of locally sourced plants is presented VHF radio-tags are deployed on crows at site-3 to document foraging to wild NC crows at site-3 alongside naturalistic NC crows at site-3 – challenging behavior – no tool manufacture recorded. foraging tasks – low task engagement. observation conditions.
Load more

Recommended publications
  • Morphological and Molecular Barcode Analysis of the Medicinal Tree Mimusops Coriacea (A.DC.) Miq
    Morphological and molecular barcode analysis of the medicinal tree Mimusops coriacea (A.DC.) Miq. collected in Ecuador Katherine Bustamante1, Efrén Santos-Ordóñez2,3, Migdalia Miranda4, Ricardo Pacheco2, Yamilet Gutiérrez5 and Ramón Scull5 1 Facultad de Ciencias Químicas, Ciudadela Universitaria “Salvador Allende,” Universidad de Guayaquil, Guayaquil, Ecuador 2 Centro de Investigaciones Biotecnológicas del Ecuador, ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador 3 Facultad de Ciencias de la Vida, ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador 4 Facultad de Ciencias Naturales y Matemáticas, ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador 5 Instituto de Farmacia y Alimentos, Universidad de La Habana, Ciudad Habana, Cuba ABSTRACT Background: Mimusops coriacea (A.DC.) Miq., (Sapotaceae), originated from Africa, were introduced to coastal areas in Ecuador where it is not extensively used as a traditional medicine to treat various human diseases. Different therapeutically uses of the species include: analgesic, antimicrobial, hypoglycemic, inflammation and pain relieve associated with bone and articulation-related diseases. Furthermore, Mimusops coriacea could be used as anti-oxidant agent. However, botanical, chemical or molecular barcode information related to this much used species is not available from Ecuador. In this study, morphological characterization was performed from leaves, stem and seeds. Furthermore, genetic characterization was performed using molecular barcodes for rbcL, matk, ITS1 and ITS2 using DNA extracted from leaves. Methods: Macro-morphological description was performed on fresh leaves, stem Submitted 25 March 2019 and seeds. For anatomical evaluation, tissues were embedded in paraffin and Accepted 29 August 2019 transversal dissections were done following incubation with sodium hypochlorite Published 11 October 2019 and safranin for coloration and fixated later in glycerinated gelatin.
    [Show full text]
  • Phytocompounds Investigation, Isolation of Flavan-3-Ol from the Stem of Manilkara Hexandra (Roxb.) Dubard and Its Potential in Anti- Oxidant
    INTERNATIONAL JOURNAL OF SCIENTIFIC & TECHNOLOGY RESEARCH VOLUME 8, ISSUE 08, AUGUST 2019 ISSN 2277-8616 Phytocompounds Investigation, Isolation Of Flavan-3-Ol From The Stem Of Manilkara Hexandra (Roxb.) Dubard And Its Potential In Anti- Oxidant S.Irudaya Monisha, J.Rosaline Vimala Abstract: The plant poly phenols are present adequately which has high impact on human drugs. Flavan- 3- ol consist in the stem of Manilkara hexandra and it has rich medicinal value like anti-oxidant. The physical and chemical properties were investigated from the stem of Manilkara hexandra. Proximate, histochemical, fluorescence, mineral analysis were examined by using standard procedures. The flavan-3-ol was isolated using chromatographics method such as coloumn chromatography and thin layer chromatography. Then it was characterized by UV, FT-IR, LC-Ms, NMR (1H & 13 C) and it is further extended to in-vitro anti-oxidant (DPPH method) potential. The flavanoid was identified by preliminary investigation after then flavan-3-ol was isolated and it gives good response for anti-oxidant activity. The isolated catechin has a unique biological behaviours, and act as a metal chelating agent. So it can be used for further research from this plant. Index Terms: Anti-Oxidant, Fluorescence , Histochemical, Isolation, Manilkara Hexandra , Mineral Analysis ,Proximate. 1 INTRODUCTION properties,such as sapotaceae [14]. The genus Manilkara includes 135 plants that are distributed worldwide. MEDICINAL plants are used in all the cultures as a basic Manilkara hexandra (Roxb.) and Manilkara zapota(L) are source for medicines.[1], [2], [3]. Plant posses’ secondary native to south Asia*15+. Plant’s minerals have nutritional metabolities which contain pharmacological and chemical importance [16] and it thus finds its applications in curing properties it is also encouraging tool for human health [4], diseases related to liver, kidney, hepatitis and cancer [5].
    [Show full text]
  • Chapter 3 Protection from Wind and Salt Spray
    CHAPTER 3 PROTECTION FROM WIND AND SALT SPRAY Thematic paper: Protective functions of coastal forests and trees against wind and salt spray Eugene S. Takle, T.-C. Chen and Xiaoqing Wu1 This paper provides an overview of the hazard potential presented by wind and salt spray to human settlements in coastal areas of Southeast Asia. Damage to infrastructure (such as buildings, dams, bridges and dykes) and degradation of natural systems in Southeast Asia and India due to wind and salt spray are closely linked to climatological conditions that lead to high wind in the region (Section 1.1). The mechanisms for generation of salt spray and their relation to windspeed are discussed in Section 1.3. Introducing shelterbelts and coastal forests is proposed as an environmentally attractive method for suppressing damage due to wind and sea spray. The properties of shelterbelts that define their effectiveness for reducing wind and capturing sea salt are described in Section 2 and the development of guidelines for their re-establishment in coastal areas is addressed in Section 3. There is a brief discussion on economics and social aspects of establishing coastal forests in Section 4, and Section 5 offers concluding remarks. 1 Wind and salt spray damage in Asia Section 1 gives an overview of wind and salt spray damage potential for Southeast Asia. Actual records or maps of past damage to human assets in the region are neither comprehensive nor widely available, particularly in the context of salt spray damage. Available data for assessing wind and salt spray damage potential in Southeast Asia are perhaps a useful surrogate and arguably more relevant for policy-making, as actual damage maps would be highly dependent on the value of coastal human assets exposed to damaging conditions.
    [Show full text]
  • Rapid Degradation of a Mauritian Rainforest Following the First 60 Years
    Rapid degradation of a Mauritian rainforest following the first 60 years of plant invasion M alika V irah-Sawmy,John M auremootoo,Doreen M arie,Saoud M otala and J ean-Claude S evathian Abstract Biological invasions by non-indigenous species (Vitousek et al., 1987; Vitousek, 1990; Ehrenfeld et al., 2001) are widely recognized as an important threat to biodiver- and hydrological cycles (Dyer & Rice, 1999; Zavaleta, 2000), sity. However, the dimension, magnitude and mechanism trophic structure and disturbance intensity and frequency of the impacts of invasive species remains poorly under- (D’Antonio, 2000; Brooks et al., 2004). stood. We assessed the role of invasive plants by compar- However, the magnitude of the impacts of most invasive ing vegetation changes that occurred between 1939 and species remains poorly documented and difficult to quan- 1999, a snapshot period that coincides with the onset of tify (Hulme, 2003). Of central importance, we still do not invasion, in Macabe´ Reserve in Mauritius. This Reserve know whether the positive correlation between native was described as biotically homogeneous in 1939. In both species decline and invasive species dominance in many surveys all native trees (. 10 DBH) were recorded from 10 ecosystems is cause or effect (Gurevitch & Padilla, 2004; 2 1,000 m random plots. In 1999 the mean richness of plant Didham et al., 2005) or whether invasive species ‘actively species in plots was significantly lower: 15 species com- suppress or exclude subordinates (natives) by lowering pared to 28 in 1939. The density and basal area of native resource availability to levels that only they can tolerate ..
    [Show full text]
  • International Journal of Modern Pharmaceutical
    IJMPR 2021, 5(4), 39-46 ISSN: 2319-5878 IJMPR Amandeep et al. International Journal International of Journal Modern of Modern Pharmaceutical Research 39 Review Article Pharmaceutical Research SJIF Impact Factor: 5.273 www.ijmpronline.com REVIEW ARTICLE ON MANILKARA HEXANDRA (KHIRNI) Amandeep Kaur* and Dr. Naresh Singh Gill Department of Pharmaceutical Chemistry, Rayat Institute of Pharmacy, Railmajra. Received on: 25/05/2021 ABSTRACT Revised on: 15/06/2021 Manilkara hexandra commonly known as Rayan and Khirni is an evergreen tree Accepted on: 05/07/2021 species with a long history of traditional medicinal uses in South Asia chiefly in western and central India, belongs to family Sapotaceae. The genus Manilkara includes *Corresponding Author 135 plants that are distributed Worldwide. Sapotaceae family consists of 58 genus and Amandeep Kaur just about 1250 species with morphological variation, ranging from shrubs to medium and giant trees. Brazil comprises of 11 genera, and 231 species, covering 1 endemic Department of genus, and 104 endemic species. The plant has been famous for its curative properties Pharmaceutical Chemistry, and has been put to use for treatment of various ailments suchlike ulcer, bronchitis, Rayat Institute of Pharmacy, jaundice, fever, hyper dyspepsia, arthritis and alimentary disorders. A record of the Railmajra. literature show extracts and metabolites from this plant having pharmacological properties such as anti–inflammatory, antiulcer, aphrodisiac, alexipharmic, anthelmintic, antibacterial, and free radical scavenging activity. Apart from medicinal uses, plant has high scale value because of its edible and nutritive fruit, useful wood, latex and bark and contributes substantial livelihood support to local inhabitants. KEYWORDS: Khirni, Manilkara hexandra, Sapotaceae, Rayan, Pharmacological properties.
    [Show full text]
  • Mise En Page 1
    CONSERVATOIRE ET JARDIN BOTANIQUES DE LA VILLE DE GENÈVE – RAPPORT ANNUEL 20 13 SOMMAIRE AVANT-PROPOS ET ÉDITORIAL ................................................................................... 2–3 STRUCTURE ET MISSIONS .......................................................................................... 4–5 LES COLLECTIONS DE NOS HERBIERS ....................................................................... 6–9 LES COLLECTIONS DE NOTRE BIBLIOTHÈQUE ........................................................ 10-11 LE JARDIN: UNE COLLECTION VIVANTE .................................................................. 12-15 DES MISSIONS D’EXPLORATION ET DE RÉCOLTE .................................................... 16-19 LES PROJETS DE RECHERCHE ................................................................................ 20-27 CONSERVATION ET PROTECTION DE LA FLORE ...................................................... 28-33 LES SYSTÈMES D’INFORMATIONS SUR LA BIODIVERSITÉ ...................................... 34-37 ÉDITIONS, ENSEIGNEMENT & FORMATION .............................................................. 38-41 ÉDUCATION ENVIRONNEMENTALE ET COMMUNICATION ........................................ 42-45 LES CENTRES HÉBERGÉS AUX CJBG ...................................................................... 46-49 INFO FLORA ................................................................................................................ 46-47 PROSPECIERARA .........................................................................................................
    [Show full text]
  • Accounting for Variation of Substitution Rates Through Time in Bayesian Phylogeny Reconstruction of Sapotoideae (Sapotaceae)
    Molecular Phylogenetics and Evolution 39 (2006) 706–721 www.elsevier.com/locate/ympev Accounting for variation of substitution rates through time in Bayesian phylogeny reconstruction of Sapotoideae (Sapotaceae) Jenny E.E. Smedmark ¤, Ulf Swenson, Arne A. Anderberg Department of Phanerogamic Botany, Swedish Museum of Natural History, P.O. Box 50007, SE-104 05 Stockholm, Sweden Received 9 September 2005; revised 4 January 2006; accepted 12 January 2006 Available online 21 February 2006 Abstract We used Bayesian phylogenetic analysis of 5 kb of chloroplast DNA data from 68 Sapotaceae species to clarify phylogenetic relation- ships within Sapotoideae, one of the two major clades within Sapotaceae. Variation in substitution rates through time was shown to be a very important aspect of molecular evolution for this data set. Relative rates tests indicated that changes in overall rate have taken place in several lineages during the history of the group and Bayes factors strongly supported a covarion model, which allows the rate of a site to vary over time, over commonly used models that only allow rates to vary across sites. Rate variation over time was actually found to be a more important model component than rate variation across sites. The covarion model was originally developed for coding gene sequences and has so far only been tested for this type of data. The fact that it performed so well with the present data set, consisting mainly of data from noncoding spacer regions, suggests that it deserves a wider consideration in model based phylogenetic inference. Repeatability of phylogenetic results was very diYcult to obtain with the more parameter rich models, and analyses with identical settings often supported diVerent topologies.
    [Show full text]
  • New Genetic Markers for Sapotaceae Phylogenomics: More Than 600 Nuclear Genes Applicable from Family to Population Levels
    Molecular Phylogenetics and Evolution 160 (2021) 107123 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev New genetic markers for Sapotaceae phylogenomics: More than 600 nuclear genes applicable from family to population levels Camille Christe a,b,*,1, Carlos G. Boluda a,b,1, Darina Koubínova´ a,c, Laurent Gautier a,b, Yamama Naciri a,b a Conservatoire et Jardin botaniques, 1292 Chamb´esy, Geneva, Switzerland b Laboratoire de botanique syst´ematique et de biodiversit´e de l’Universit´e de Gen`eve, Department of Botany and Plant Biology, 1292 Chamb´esy, Geneva, Switzerland c Laboratory of Evolutionary Genetics, Institute of Biology, University of Neuchatel,^ Rue Emile-Argand 11, 2000 Neuchatel,^ Switzerland ARTICLE INFO ABSTRACT Keywords: Some tropical plant families, such as the Sapotaceae, have a complex taxonomy, which can be resolved using Conservation Next Generation Sequencing (NGS). For most groups however, methodological protocols are still missing. Here Gene capture we identified531 monocopy genes and 227 Short Tandem Repeats (STR) markers and tested them on Sapotaceae STR using target capture and NGS. The probes were designed using two genome skimming samples from Capur­ Phylogenetics odendron delphinense and Bemangidia lowryi, both from the Tseboneae tribe, as well as the published Manilkara Population genetics Species tree zapota transcriptome from the Sapotoideae tribe. We combined our probes with 261 additional ones previously Tropical trees published and designed for the entire angiosperm group. On a total of 792 low-copy genes, 638 showed no signs of paralogy and were used to build a phylogeny of the family with 231 individuals from all main lineages.
    [Show full text]
  • Taxonomic Revision of the Genus Manilkara ( Sapotaceae) in Madagascar
    E D I N B U R G H J O U R N A L O F B O T A N Y 65 (3): 433–446 (2008) 433 Ó Trustees of the Royal Botanic Garden Edinburgh (2008) doi:10.1017/S096042860800485X TAXONOMIC REVISION OF THE GENUS MANILKARA ( SAPOTACEAE) IN MADAGASCAR V. PLANA1 &L.GAUTIER2 A revision of the five Madagascan species of the genus Manilkara (Sapotaceae)is presented, including a key, descriptions, diagnostic characters, ecological notes and a distribution map. Of the seven species originally described by Aubre´ville, Manilkara tampoloensis is placed in synonymy with M. boivinii, and M. sohihy is removed from the genus and placed within the existing Labramia boivinii (Pierre) Aubre´v. Keywords. Madagascar, Manilkara, Sapotaceae, taxonomic revision. Introduction The genus Manilkara Adans., probably best known for American species such as M. zapota (sapodilla) and M. chicle (chicle), is a pantropical genus comprising c.82 species (Govaerts et al., 2001). Of these, approximately one third are found in Africa (Plana, in prep.) and Madagascar. Although the Madagascan species of Manilkara share some characteristics with mainland African species, none are found in Africa. Afro-Madagascan species can be divided, according to their gross morphology, into three broad biogeographic regions: Madagascar, East and South Africa, and Central and West Africa. Malagasy species share characteristics with species in both regions, where they are commonly constituents of evergreen forest. Manilkara is one of six genera constituting the subtribe Manilkarinae H.J.Lam (tribe Mimusopeae Hartog) (Pennington, 1991) which also includes Labramia A.DC., Faucherea Lecomte, Northia Hook.f., Labourdonnaisia Bojer and Letestua Lecomte.
    [Show full text]
  • Inhibition of SARS-Cov-2 Main Protease by Phenolic Compounds
    RSC Advances View Article Online PAPER View Journal | View Issue Inhibition of SARS-CoV-2 main protease by phenolic compounds from Manilkara hexandra Cite this: RSC Adv., 2020, 10,32148 (Roxb.) Dubard assisted by metabolite profiling and in silico virtual screening† Fatma M. Abd El-Mordy, a Mohamed M. El-Hamouly,b Magda T. Ibrahim,c Gehad Abd El-Rheem,d Omar M. Aly,e Adel M. Abd El-kader,fg Khayrya A. Youssifh and Usama Ramadan Abdelmohsen *fi SARS-CoV-2 is a novel coronavirus that was first identified during the outbreak in Wuhan, China in 2019. It is an acute respiratory illness that can transfer among human beings. Natural products can provide a rich resource for novel antiviral drugs. They can interfere with viral proteins such as viral proteases, polymerases, and entry proteins. Several naturally occurring flavonoids were reported to have antiviral activity against different types of RNA and DNA viruses. A methanolic extract of Manilkara hexandra Creative Commons Attribution 3.0 Unported Licence. (Roxb.) Dubard leaves is rich in phenolic compounds, mainly flavonoids. Metabolic profiling of the secondary metabolites of Manilkara hexandra (Roxb.) Dubard leaves methanolic extract (MLME), and bark ethyl acetate (MBEE) extract using LC-HRESIMS resulted in the isolation of 18 compounds belonging to a variety of constituents, among which phenolic compounds, flavones, flavonol glycosides and triterpenes were predominant. Besides, four compounds (I–IV) were isolated and identified as myricetin I, myricitrin II, mearnsitrin III, and mearnsetin-3-O-b-D-rutinoside IV (compound IV is isolated for the first time from genus Manilkara) and dereplicated in a metabolomic study as compounds 3, 5, 6, and 12, respectively.
    [Show full text]
  • The Genus Manilkara
    The Pharma Innovation Journal 2018; 7(1): 316-318 ISSN (E): 2277- 7695 ISSN (P): 2349-8242 NAAS Rating: 5.03 The genus Manilkara: An update TPI 2018; 7(1): 316-318 © 2018 TPI www.thepharmajournal.com Anjali, Vandana Garg, Anju Dhiman, Rohit Dutt and Sweety Ranga Received: 21-11-2017 Accepted: 22-12-2017 Abstract Anjali The genus Manilkara includes 135 plants that distributed Worldwide. In this review we had discussed Department of Pharmaceutical three popular plants from Genus Manilkara. i.e. M. bidentata (A.DC.) is native to South America. M. Sciences, Maharshi Dayanand hexandra (Roxb.) and M. zapota (L.) are native to South Asia. Above mentioned species from Manilkara University, Rohtak, Haryana, i.e. M. hexandra and M. zapota are known for their medicinal properties and pleasant taste. Traditionally India these species are used in wound healing, inflammation and fever. All the three species of the Genus have been largely explored for their anticancer and antibacterial activities. Vandana Garg Department of Pharmaceutical Sciences, Maharshi Dayanand Keywords: M. zapota, M. hexandra, ethnopharmacology, anticancer, antibacterial University, Rohtak, Haryana, India 1. Introduction Literature of Genus Manilkara is organized from the chief directory such as Taylor & Francis, Anju Dhiman Forest Products Laboratory, Chemical abstracts, Annals of Phytomedicine, Scholars Research Department of Pharmaceutical Sciences, Maharshi Dayanand Library, PubMed, Research Gate, Elsevier, Academic Sciences, Pharma Scholars using as University, Rohtak, Haryana, references. India The given data of three species from Manilkara is categorized in to four parts i.e. Ethnopharmacology, morphology, chemical constituents and pharmacological activities. The Rohit Dutt ethnopharmacological uses includes its traditional and other medicinal uses.
    [Show full text]
  • Effect of Leaf Aqueous Extract of Manilkara Hexandra on Digestive and Glucose Metabolic Enzymes, and Serum Glucose Level in Labeo Rohita Fingerlings
    bioRxiv preprint doi: https://doi.org/10.1101/2021.01.17.426992; this version posted January 19, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Effect of Leaf Aqueous Extract of Manilkara hexandra on Digestive and Glucose Metabolic Enzymes, and Serum Glucose Level in Labeo rohita Fingerlings Sumana Dutta1,3, Debnarayan Chowdhury2, Ria Das1, Jayashri Das2, Poulomi Ghosh1, Koushik Ghosh2,**, Sanjib Ray1,* 1 Molecular Biology and Genetics Unit, Department of Zoology, The University of Burdwan, Golapbag, Purba Bardhaman-713104, West Bengal, India. 2 Aquaculture Laboratory, Department of Zoology, The University of Burdwan, Golapbag, Purba Bardhaman- 713104, West Bengal, India. 3 Durgapur Govt. College, Durgapur- 713214, West Bengal, India. * Corresponding author; Email address: [email protected] ** Co-corresponding author; Email address: [email protected] Abstract Manilkara hexandra (Roxb.) Dubard (common name Khirni, family: Sapotaceae) is an evergreen traditionally used medicinal plant. The present study aimed to see the effects of leaf aqueous extract of M. hexandra (LAEMH) on digestive and glucose metabolic enzyme action and serum glucose level in rohu, Labeo rohita, fingerlings. Experimental fish were fed a basal diet (Group I), and diets supplemented with LAEMH at 300 mg (Group II) and 600 mg (Group III) kg-1 body weight. A significant reduction in serum glucose was noticed in the treated groups when compared with the control group. The reduced amylase activity was noticed in the treated fingerlings in a dose-dependent manner. However, lipase and protease activities didn’t differ significantly among the experimental groups.
    [Show full text]