Supplementary Table 1. Regulated in mice after DHT and E2

Probe Set Title Gene Symbol Fold Change Down/Up P

Genes regulated after DHT

1419487_at binding H Mybph 11.9 down 0.000

1415927_at . alpha. cardiac Actc1 3.8 down 0.000

1419606_a_at T1. skeletal. slow Tnnt1 2.8 down 0.004

1448553_at myosin. heavy polypeptide 7. cardiac muscle. beta Myh7 2.6 down 0.001

1417765_a_at amylase 1. salivary Amy1 2.5 down 0.000

1416411_at glutathione S-transferase. mu 2 Gstm2 2.5 down 0.000

1418492_at gremlin 2 homolog. cysteine knot superfamily (Xenopus laevis) Grem2 2.4 down 0.000

1424392_at alcohol dehydrogenase. iron containing. 1 Adhfe1 2.2 down 0.000

1418370_at . cardiac/slow skeletal Tnnc1 2.2 down 0.002

1448394_at myosin. light polypeptide 2. regulatory. cardiac. slow Myl2 2.2 down 0.004

1450813_a_at . skeletal. slow 1 Tnni1 2.1 down 0.001

1449996_a_at 3. gamma Tpm3 2.1 down 0.017

1449118_at dihydrolipoamide branched chain transacylase E2 Dbt 1.8 down 0.002

1418507_s_at suppressor of cytokine signaling 2 Socs2 1.8 down 0.038

1449059_a_at 3-oxoacid CoA transferase 1 Oxct1 1.8 down 0.000

1453552_at RIKEN cDNA 2310014F07 gene 2310014F07Rik 1.8 down 0.012

1436913_at CDC14 cell division cycle 14 homolog A (S. cerevisiae) Cdc14a 1.8 down 0.014

1448554_s_at myosin. heavy polypeptide 6. cardiac muscle. alpha Myh6 1.7 down 0.008

1427168_a_at procollagen. type XIV. alpha 1 Col14a1 1.7 down 0.005

1423607_at lumican Lum 1.7 down 0.005 1451310_a_at cathepsin L Ctsl 1.7 down 0.001

1452363_a_at ATPase. Ca++ transporting. cardiac muscle. slow twitch 2 Atp2a2 1.7 down 0.002

1452203_at oligonucleotide/oligosaccharide-binding fold containing 2A Obfc2a 1.7 down 0.007

1420523_at coiled-coil domain containing 50 Ccdc50 1.6 down 0.008

1417719_at sin3 associated polypeptide Sap30 1.6 down 0.007

1418152_at nucleosome binding protein 1 Nsbp1 1.6 down 0.012

1424649_a_at tetraspanin 8 Tspan8 1.6 down 0.003

1425090_s_at potassium voltage gated channel. Shaw-related subfamily. member 4 Kcnc4 1.6 down 0.001

1421891_at ST3 beta-galactoside alpha-2.3-sialyltransferase 2 St3gal2 1.6 down 0.014

1455007_s_at glutamic pyruvate transaminase (alanine aminotransferase) 2 Gpt2 1.6 down 0.004

1427343_at RASD family. member 2 Rasd2 1.6 down 0.014

1460451_at transmembrane protein 52 Tmem52 1.6 down 0.003

1427769_x_at myosin. light polypeptide 3 Myl3 1.6 down 0.002

1423606_at periostin. osteoblast specific factor Postn 1.6 down 0.025

1443299_at PDZ and LIM domain 3 (Pdlim3). mRNA Pdlim3 1.5 down 0.011

1426236_a_at glutamate-ammonia ligase (glutamine synthetase) Glul 1.5 down 0.014

1438855_x_at tumor necrosis factor. alpha-induced protein 2 Tnfaip2 1.5 down 0.028

1448181_at Kruppel-like factor 15 Klf15 1.5 down 0.002

1451047_at integral membrane protein 2A Itm2a 1.5 down 0.000

1425458_a_at growth factor receptor bound protein 10 Grb10 1.5 down 0.001

1424151_at JTV1 gene Jtv1 1.5 down 0.000

1450637_a_at AE binding protein 1 Aebp1 1.5 down 0.000

1451031_at secreted frizzled-related sequence protein 4 Sfrp4 1.5 down 0.001

1427153_at branched chain ketoacid dehydrogenase E1. beta polypeptide Bckdhb 1.5 down 0.003 1437273_at HtrA serine peptidase 4 Htra4 1.5 down 0.004

1422982_at androgen receptor Ar 1.5 down 0.001

1435148_at ATPase. Na+/K+ transporting. beta 2 polypeptide Atp1b2 1.5 down 0.000

1433711_s_at sestrin 1 Sesn1 1.5 down 0.000

1438641_x_at RIKEN cDNA 1500016O10 gene 1500016O10Rik 1.5 down 0.041

1433924_at ------1.6 down 0.002

1436092_at ------1.7 down 0.000

AFFX-18SRNAMur/X00686_M_at 1.5 down 0.029

AFFX-18SRNAMur/X00686_3_at 1.6 down 0.007

AFFX-18SRNAMur/X00686_5_at 3.4 down 0.003

1451601_a_at spinster homolog 2 (Drosophila) Spns2 3.4 up 0.000

1455031_at cell division cycle 2-like 6 (CDK8-like) Cdc2l6 3.0 up 0.000

1427339_at solute carrier family 30 (zinc transporter). member 2 Slc30a2 3.0 up 0.000

1427364_a_at ornithine decarboxylase. structural 1 Odc1 2.8 up 0.000

1424268_at spermine oxidase Smox 2.6 up 0.000

1427722_at RIKEN cDNA 9830102E05 gene 9830102E05Rik 2.6 up 0.001

1419423_at stabilin 2 Stab2 2.5 up 0.000

1455898_x_at solute carrier family 2 (facilitated glucose transporter). member 3 Slc2a3 2.5 up 0.000

1455377_at tyrosine ligase-like family. member 7 Ttll7 2.5 up 0.000

1448881_at haptoglobin Hp 2.4 up 0.020

1457587_at Voltage-gated potassium channel KCNQ5 (Kcnq5) Kcnq5 2.3 up 0.001

1451465_at ubiquitin-like 7 (bone marrow stromal cell-derived) Ubl7 2.3 up 0.000

1416835_s_at S-adenosylmethionine decarboxylase 1 /// S-adenosylmethionine decarboxylase 2 Amd1 /// Amd2 2.3 up 0.000

1460241_a_at ST3 beta-galactoside alpha-2.3-sialyltransferase 5 St3gal5 2.3 up 0.000 1428111_at solute carrier family 38. member 4 Slc38a4 2.3 up 0.010

1418509_at carbonyl reductase 2 Cbr2 2.2 up 0.000

1452035_at procollagen. type IV. alpha 1 Col4a1 2.2 up 0.000

1416138_at annexin A7 Anxa7 2.2 up 0.000

1438676_at macrophage activation 2 like Mpa2l 2.2 up 0.002

1448747_at F-box only protein 32 Fbxo32 2.1 up 0.004

1455794_at smoothelin-like 2 Smtnl2 2.1 up 0.000

1417611_at transmembrane protein 37 Tmem37 2.1 up 0.013

1435605_at ARP3 actin-related protein 3 homolog B (yeast) Actr3b 2.1 up 0.001

1424514_at ring finger protein 126 Rnf126 2.0 up 0.000

1450138_a_at serine (or cysteine) peptidase inhibitor. clade B. member 6a Serpinb6a 2.0 up 0.000

1418003_at RIKEN cDNA 1190002H23 gene 1190002H23Rik 2.0 up 0.004

1434548_at serine incorporator 3 Serinc3 2.0 up 0.002

1418091_at transcription factor CP2-like 1 Tcfcp2l1 1.9 up 0.002

1455399_at connector enhancer of kinase suppressor of Ras 1 Cnksr1 1.9 up 0.001

1433702_at endoplasmic reticulum metallopeptidase 1 Ermp1 1.9 up 0.016

1418697_at indolethylamine N-methyltransferase Inmt 1.9 up 0.002

1415897_a_at microsomal glutathione S-transferase 1 Mgst1 1.9 up 0.004

1424854_at histone 1. H4i Hist1h4i 1.9 up 0.008

1436309_at neuropilin (NRP) and tolloid (TLL)-like 2 Neto2 1.9 up 0.002

1424051_at procollagen. type IV. alpha 2 Col4a2 1.9 up 0.001

1432001_at zinc finger. MYND domain containing 17 Zmynd17 1.9 up 0.024

1415698_at golgi phosphoprotein 2 Golph2 1.9 up 0.002

1453851_a_at growth arrest and DNA-damage-inducible 45 gamma Gadd45g 1.9 up 0.002 1435902_at nudix (nucleoside diphosphate linked moiety X)-type motif 18 Nudt18 1.9 up 0.005

1426464_at nuclear receptor subfamily 1. group D. member 1 Nr1d1 1.9 up 0.015

1448300_at microsomal glutathione S-transferase 3 Mgst3 1.8 up 0.000

1449005_at solute carrier family 16 (monocarboxylic acid transporters). member 3 Slc16a3 1.8 up 0.000

1427883_a_at procollagen. type III. alpha 1 Col3a1 1.8 up 0.013

1423086_at Niemann Pick type C1 Npc1 1.8 up 0.012

1451342_at spondin 1. (f-spondin) extracellular matrix protein Spon1 1.8 up 0.000

1423296_at proteasome (prosome. macropain) 26S subunit. non-ATPase. 8 Psmd8 1.8 up 0.000

1452661_at transferrin receptor Tfrc 1.8 up 0.002

1416125_at FK506 binding protein 5 Fkbp5 1.8 up 0.002

1424126_at aminolevulinic acid synthase 1 Alas1 1.8 up 0.001

1427992_a_at RAB12. member RAS oncogene family Rab12 1.7 up 0.000

1436715_s_at CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol synthase) Cdipt 1.7 up 0.003

1434947_at family member 3C Kif3c 1.7 up 0.001

1423110_at collagen. type I. alpha 2 Col1a2 1.7 up 0.049

1431213_a_at hypothetical gene LOC433762 LOC433762 1.7 up 0.003

1424795_a_at RIKEN cDNA 1700001O22 gene 1700001O22Rik 1.7 up 0.003

1416514_a_at fascin homolog 1. actin bundling protein (Strongylocentrotus) purpuratus) Fscn1 1.7 up 0.011

1455628_at erythrocyte protein band 4.1-like 4b Epb4.1l4b 1.7 up 0.013

1417562_at eukaryotic translation initiation factor 4E binding protein 1 Eif4ebp1 1.7 up 0.000

1417279_at inositol 1.4.5-triphosphate receptor 1 Itpr1 1.7 up 0.004

1428357_at RIKEN cDNA 2610019F03 gene 2610019F03Rik 1.7 up 0.000

1422437_at procollagen. type V. alpha 2 Col5a2 1.7 up 0.015

1448566_at solute carrier family 40 (iron-regulated transporter). member 1 Slc40a1 1.7 up 0.000 1450914_at protein phosphatase 1. regulatory (inhibitor) subunit 14B Ppp1r14b 1.7 up 0.000

1453898_at integrin beta 1 binding protein 3 Itgb1bp3 1.7 up 0.043

1428536_at potassium voltage-gated channel. subfamily G. member 4 Kcng4 1.7 up 0.003

1421413_a_at PDZ and LIM domain 5 Pdlim5 1.7 up 0.000

1423596_at NIMA (never in mitosis gene a)-related expressed kinase 6 Nek6 1.7 up 0.002

1455493_at synaptic nuclear envelope 1 Syne1 1.7 up 0.001

1440085_at ectodysplasin A2 isoform receptor Eda2r 1.6 up 0.037

1416589_at secreted acidic cysteine rich glycoprotein Sparc 1.6 up 0.003

1426951_at cysteine rich transmembrane BMP regulator 1 (chordin like) Crim1 1.6 up 0.022

1435950_at hairless Hr 1.6 up 0.001

1449533_at transmembrane protein 100 Tmem100 1.6 up 0.033

1422324_a_at parathyroid hormone-like peptide Pthlh 1.6 up 0.001

1417409_at Jun oncogene Jun 1.6 up 0.011

1455494_at procollagen. type I. alpha 1 Col1a1 1.6 up 0.044

1429273_at BMP-binding endothelial regulator Bmper 1.6 up 0.001

1424842_a_at Rho GTPase activating protein 24 Arhgap24 1.6 up 0.004

1438426_at shisa homolog 4 (Xenopus laevis) Shisa4 1.6 up 0.005

1455548_at discs. large homolog-associated protein 4 (Drosophila) Dlgap4 1.6 up 0.007

1433590_at hect domain and RLD 3 Herc3 1.6 up 0.015

1440715_s_at CDKN2A interacting protein N-terminal like Cdkn2aipnl 1.6 up 0.018

1421425_a_at regulator of calcineurin 2 Rcan2 1.6 up 0.006

1422664_at RAB10. member RAS oncogene family Rab10 1.6 up 0.004

1423600_a_at Treacher Collins Franceschetti syndrome 1. homolog Tcof1 1.6 up 0.000

1449002_at pleckstrin homology-like domain. family A. member 3 Phlda3 1.6 up 0.000 1429900_at RIKEN cDNA 5330406M23 gene 5330406M23Rik 1.6 up 0.018

1438251_x_at HtrA serine peptidase 1 Htra1 1.6 up 0.002

1448669_at dickkopf homolog 3 (Xenopus laevis) Dkk3 1.6 up 0.025

1438258_at very low density lipoprotein receptor Vldlr 1.6 up 0.002

1440343_at ribosomal protein S6 kinase. polypeptide 5 Rps6ka5 1.6 up 0.029

1450511_at muscle. skeletal. receptor tyrosine kinase Musk 1.6 up 0.011

1440559_at high mobility group AT-hook 2. pseudogene 1 Hmga2-ps1 1.6 up 0.006

1436984_at abl-interactor 2 Abi2 1.6 up 0.019

1454758_a_at TSC22 domain family. member 1 Tsc22d1 1.6 up 0.000

1430036_at RIKEN cDNA 2310015B20 gene 2310015B20Rik 1.6 up 0.000

1438651_a_at angiotensin receptor-like 1 Agtrl1 1.6 up 0.017

1417533_a_at integrin beta 5 Itgb5 1.6 up 0.001

1451290_at -associated protein 1 light chain 3 alpha Map1lc3a 1.6 up 0.000

1417283_at Ly6/neurotoxin 1 Lynx1 1.6 up 0.000

1429918_at Rho GTPase activating protein 20 Arhgap20 1.5 up 0.044

1448959_at NADH dehydrogenase (ubiquinone) Fe-S protein 4 Ndufs4 1.5 up 0.000

1424719_a_at microtubule-associated protein tau Mapt 1.5 up 0.001

1455354_at WD repeat domain 22 Wdr22 1.5 up 0.002

1450940_at ganglioside-induced differentiation-associated-protein 1 Gdap1 1.5 up 0.044

1456413_at phosphodiesterase 4D interacting protein (myomegalin) Pde4dip 1.5 up 0.017

1456929_at CDNA sequence BC042782 BC042782 1.5 up 0.006

1415932_x_at ATPase. class II. type 9A Atp9a 1.5 up 0.000

1417168_a_at ubiquitin specific peptidase 2 Usp2 1.5 up 0.045

1449029_at MAP kinase-interacting serine/threonine kinase 2 Mknk2 1.5 up 0.001 1417389_at glypican 1 Gpc1 1.5 up 0.001

1451225_at protein tyrosine phosphatase. non-receptor type 11 Ptpn11 1.5 up 0.000

1417622_at solute carrier family 12. member 2 Slc12a2 1.5 up 0.008

1418395_at solute carrier family 47. member 1 Slc47a1 1.5 up 0.009

1422693_a_at SUB1 homolog (S. cerevisia Sub1 1.5 up 0.000

1429593_at solute carrier family 38. member 2 Slc38a2 1.5 up 0.004

1450505_a_at RIKEN cDNA 1810015C04 gene 1810015C04Rik 1.5 up 0.001

1435888_at epidermal growth factor receptor Egfr 1.5 up 0.007

1421321_a_at neuroepithelial cell transforming gene 1 Net1 1.5 up 0.002

1457883_at ------1.5 up 0.025

1456570_at ------1.6 up 0.004

1455418_at ------2.1 up 0.019

1433898_at ------3.3 up 0.002

1437073_x_at ------4.1 up 0.004

AFFX-r2-Ec-bioD-5_at 1.5 up 0.001

AFFX-r2-Ec-bioB-5_at 1.6 up 0.001

AFFX-r2-Ec-bioB-3_at 1.6 up 0.000

AFFX-BioC-5_at 1.6 up 0.000

AFFX-BioB-3_at 1.6 up 0.000

AFFX-r2-Ec-bioB-M_at 1.6 up 0.000

AFFX-BioDn-5_at 1.6 up 0.000

AFFX-r2-Ec-bioC-5_at 1.7 up 0.000

AFFX-BioB-M_at 1.7 up 0.001 Genes regulated after E2 1423506_a_at neuronatin Nnat 2.1 down 0.016

1438855_x_at tumor necrosis factor. alpha-induced protein 2 Tnfaip2 1.9 down 0.012

1422651_at adiponectin. C1Q and collagen domain containing Adipoq 1.9 down 0.026

1415964_at stearoyl-Coenzyme A desaturase 1 Scd1 1.8 down 0.005

1439332_at DNA-damage-inducible transcript 4-like Ddit4l 1.7 down 0.011

1418457_at chemokine (C-X-C motif) ligand 14 Cxcl14 1.6 down 0.001

1418492_at gremlin 2 homolog. cysteine knot superfamily (Xenopus laevis) Grem2 1.6 down 0.013

1416601_a_at Down syndrome critical region homolog 1 (human) Dscr1 1.6 down 0.002

1421811_at thrombospondin 1 /// similar to thrombospondin 1 Thbs1 1.6 down 0.021

1434413_at Transcribed 1.6 down 0.006

1425400_a_at Cbp/p300-interacting transactivator. with Glu/Asp-rich carboxy-terminal domain. 4 Cited4 1.6 down 0.013

1415939_at fibromodulin Fmod 1.6 down 0.023

1437082_at A kinase (PRKA) anchor protein (yotiao) 9 Akap9 1.6 down 0.001

1423852_at transmembrane protein 46 Tmem46 1.6 down 0.029

1418188_a_at metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA) Malat1 1.6 down 0.001

1437273_at HtrA serine peptidase 4 Htra4 1.6 down 0.044

1427768_s_at myosin. light polypeptide 3 Myl3 1.5 down 0.025

1417649_at cyclin-dependent kinase inhibitor 1C (P57) Cdkn1c 1.5 down 0.029

1420465_s_at major urinary protein 1 /// major urinary protein 2 Mup1 /// Mup2 3.4 up 0.049

1416383_a_at pyruvate carboxylase Pcx 3.1 up 0.002

1457587_at Voltage-gated potassium channel KCNQ5 (Kcnq5) Kcnq5 2.6 up 0.007

1447016_at TBC1 domain family. member 1 Tbc1d1 2.3 up 0.006

1449106_at glutathione peroxidase 3 Gpx3 2.2 up 0.001

1428352_at arrestin domain containing 2 Arrdc2 1.9 up 0.002 1452160_at TCDD-inducible poly(ADP-ribose) polymerase Tiparp 1.9 up 0.004

1415768_a_at ubiquitin-conjugating enzyme E2R 2 Ube2r2 1.9 up 0.001

1416125_at FK506 binding protein 5 Fkbp5 1.8 up 0.003

1433110_at RIKEN cDNA 5830474E16 gene 5830474E16Rik 1.7 up 0.008

1431805_a_at rhophilin. Rho GTPase binding protein 2 Rhpn2 1.7 up 0.009

1435125_at expressed sequence BB120497 BB120497 1.7 up 0.007

1453588_at carbonic anhydrase 3 Car3 1.7 up 0.044

1440715_s_at DNA segment. Chr 11. ERATO Doi 497. expressed D11Ertd497e 1.6 up 0.004

1418219_at interleukin 15 Il15 1.6 up 0.005

1458635_at RIKEN cDNA 4832428D23 gene 4832428D23Rik 1.6 up 0.000

1454867_at meningioma 1 Mn1 1.6 up 0.005

1449088_at fructose bisphosphatase 2 Fbp2 1.6 up 0.001

1447767_at Transcribed locus 1.6 up 0.010

1417083_at Sec61 beta subunit Sec61b 1.6 up 0.008

1417522_at F-box only protein 32 Fbxo32 1.6 up 0.019

1429900_at RIKEN cDNA 5330406M23 gene 5330406M23Rik 1.6 up 0.000

1435902_at nudix (nucleoside diphosphate linked moiety X)-type motif 18 Nudt18 1.6 up 0.009

1435370_a_at carboxylesterase 3 Ces3 1.6 up 0.006

1456413_at RIKEN cDNA 9430063L05 gene 9430063L05Rik 1.6 up 0.003

1439066_at angiopoietin 1 Angpt1 1.6 up 0.046

1447614_at Transcribed locus 1.6 up 0.002

1435325_at ubiquitin specific peptidase 46 Usp46 1.5 up 0.004

1440343_at ribosomal protein S6 kinase. polypeptide 5 Rps6ka5 1.5 up 0.007

1454937_at RIKEN cDNA B630005N14 gene B630005N14Rik 1.5 up 0.007 1435605_at ARP3 actin-related protein 3 homolog B (yeast) Actr3b 1.5 up 0.006

1446560_at protease. serine. 23 Prss23 1.5 up 0.015

1429206_at ------2.3 up 0.006

Microarray analyses of m. gastrocnemius from 12-week-old male mice that were either sham-operated or orchidectomized (orx) and treated for 5 weeks with vehicle (veh), dihydrotestosterone (DHT) (45 µg/day) or 17β-estradiol (E2) (0.05 µg/day). A gene was considered regulated if it demonstrated a fold change of ≥1.5 and p≤0.05 (t-test) in response to E2 or DHT treatment compared to vehicle, n=5 in each group.