SNAPAP Antibody Cat

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SNAPAP Antibody Cat. No.: XW-8015 SNAPAP Antibody Specifications HOST SPECIES: Chicken SPECIES REACTIVITY: Human, Mouse, Rat IMMUNOGEN: 31-136 TESTED APPLICATIONS: WB SNARE-associated protein Snapin antibody can be used for the detection of SNARE- APPLICATIONS: associated protein Snapin by Western Blot. PREDICTED MOLECULAR 14.9 kDa (calculated) WEIGHT: Properties PURIFICATION: Immunoaffinity Purified CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Phosphate-Buffered Saline. No preservatives added. CONCENTRATION: 1 mg/mL October 1, 2021 1 https://www.prosci-inc.com/snapap-antibody-8015.html SNAPAP antibody can be stored at 4˚C for short term (weeks). Long term storage should STORAGE CONDITIONS: be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: SNAPIN BLOC1S7, SNAP25BP, SNAPAP, SNARE-associated protein Snapin, Biogenesis of lysosome- related organelles complex 1 subunit 7, BLOC-1 subunit 7, BLOS7, BLOC1S7, ALTERNATE NAMES: SNAREassociated protein Snapin, Synaptosomal-associated protein 25 binding protein, SNAP-associated protein ACCESSION NO.: NP_036569.1 PROTEIN GI NO.: 6912674 GENE ID: 23557 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References FUNCTION: May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state BACKGROUND: influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non- neuronal cells (By similarity). ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 2 https://www.prosci-inc.com/snapap-antibody-8015.html.

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Supplementary Table 1 Genes Tested in Qrt-PCR in Nfpas
Supplementary Table 1 Genes tested in qRT-PCR in NFPAs Gene Bank accession Gene Description number ABI assay ID a disintegrin-like and metalloprotease with thrombospondin type 1 motif 7 ADAMTS7 NM_014272.3 Hs00276223_m1 Rho guanine nucleotide exchange factor (GEF) 3 ARHGEF3 NM_019555.1 Hs00219609_m1 BCL2-associated X protein BAX NM_004324 House design Bcl-2 binding component 3 BBC3 NM_014417.2 Hs00248075_m1 B-cell CLL/lymphoma 2 BCL2 NM_000633 House design Bone morphogenetic protein 7 BMP7 NM_001719.1 Hs00233476_m1 CCAAT/enhancer binding protein (C/EBP), alpha CEBPA NM_004364.2 Hs00269972_s1 coxsackie virus and adenovirus receptor CXADR NM_001338.3 Hs00154661_m1 Homo sapiens Dicer1, Dcr-1 homolog (Drosophila) (DICER1) DICER1 NM_177438.1 Hs00229023_m1 Homo sapiens dystonin DST NM_015548.2 Hs00156137_m1 fms-related tyrosine kinase 3 FLT3 NM_004119.1 Hs00174690_m1 glutamate receptor, ionotropic, N-methyl D-aspartate 1 GRIN1 NM_000832.4 Hs00609557_m1 high-mobility group box 1 HMGB1 NM_002128.3 Hs01923466_g1 heterogeneous nuclear ribonucleoprotein U HNRPU NM_004501.3 Hs00244919_m1 insulin-like growth factor binding protein 5 IGFBP5 NM_000599.2 Hs00181213_m1 latent transforming growth factor beta binding protein 4 LTBP4 NM_001042544.1 Hs00186025_m1 microtubule-associated protein 1 light chain 3 beta MAP1LC3B NM_022818.3 Hs00797944_s1 matrix metallopeptidase 17 MMP17 NM_016155.4 Hs01108847_m1 myosin VA MYO5A NM_000259.1 Hs00165309_m1 Homo sapiens nuclear factor (erythroid-derived 2)-like 1 NFE2L1 NM_003204.1 Hs00231457_m1 oxoglutarate (alpha-ketoglutarate)
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Supplementary Table 2
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Data Sheet 148
Rudolf-Wissell-Str. 28a Background 37079 Göttingen, Germany Phone: +49 551-50556-0 Snapin, also referred to as Snapap, was initially identified as a SNAP 25 interacting protein which Fax: +49 551-50556-384 enhances the binding of synaptotagmin 1 to SNAREs in a phosphorylation dependent manner. Later E-mail: [email protected] an ubiquitous expression pattern in neuronal and non-neuronal cells and interaction with SNAP 23 Web: www.sysy.com was described. The protein contains heptad repeats typical for coiled coils in its C-terminal part. Snapin The role of this protein in SNARE mediated fusion is still under discussion. Cat.No. 148 002; Polyclonal rabbit antibody, 200 µl antiserum (lyophilized) Selected References for 148 002 Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells. Data Sheet Kinoshita SM, Kogure A, Taguchi S, Nolan GP PloS one (2013) 810: e75297. WB, IP, ICC; tested species: human Reconstitution/ 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells. Storage store at -20°C until use. Ginter-Matuszewska B, Spik A, Rembiszewska A, Koyias C, Kupryjanczyk J, Jaruzelska J For detailed information, see back of the data sheet. Molecular human reproduction (2009) 153: 173-9. WB, IHC-P; tested species: human,mouse Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3) promotes immunity to Applications WB: 1 : 100 up to 1 : 5000 mycobacteria.
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Supplementary Figure S1. Generation of Floxed Nphs2 Exon 2 Allele and Confirmation
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