RESEARCH ARTICLE Pollen morphology and variability of invasive Spiraea tomentosa L. (Rosaceae) from populations in Poland 1 1 2 Dorota Wrońska-PilarekID *, Blanka Wiatrowska , Jan BocianowskiID 1 Department of Forest Botany, Poznan University of Life Sciences, Poznań, Poland, 2 Department of Mathematical and Statistical Methods, Poznan University of Life Sciences, Poznań, Poland *
[email protected] Abstract a1111111111 The aim of this study was to investigate the pollen morphology and the ranges of intraspe- a1111111111 a1111111111 cific and interindividual variability of the North American steeplebushÐSpiraea tomentosa a1111111111 L., an invasive species in Poland. Steeplebush inflorescences were collected randomly from a1111111111 30 localities of S. tomentosa in Poland. In total, 900 pollen grains were analysed with both a light and a scanning electron microscope. Nine quantitative and three qualitative pollen fea- tures were studied. The diagnostic features were: exine ornamentation (size and direction of the muri), operculum and perforation size. For the first time, the intraspecific and interindivid- OPEN ACCESS ual variability of the pollen grains of the highly invasive S. tomentosa were investigated. Pol- Citation: Wrońska-Pilarek D, Wiatrowska B, len grain features were so similar, that they did not allow to differentiate individual samples Bocianowski J (2019) Pollen morphology and of S. tomentosa and only groups of samples were recognized. variability of invasive Spiraea tomentosa L. (Rosaceae) from populations in Poland. PLoS ONE 14(8): e0218276. https://doi.org/10.1371/journal. pone.0218276 Editor: Ramasamy Perumal, Kansas State Introduction University, UNITED STATES The genus L. belongs to the Juss. family, to the Amygdaloideae subfamily and Received: February 4, 2019 Spiraea Rosaceae to the Spiraeeae tribe, which is the subject of considerable speculation regarding its internal Accepted: May 29, 2019 divisions.