DOCSLIB.ORG

Transformation of 'Galia' Melon to Improve Fruit

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Transformation of 'Galia' Melon to Improve Fruit TRANSFORMATION OF ‘GALIA’ MELON TO IMPROVE FRUIT QUALITY By HECTOR GORDON NUÑEZ-PALENIUS A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2005 Copyright 2005 by Hector Gordon Nuñez-Palenius This document is dedicated to the seven reasons in my life, who make me wake up early all mornings, work hard in order to achieve my objectives, dream on new horizons and goals, feel the beaty of the wind, rain and sunset, but mostly because they make me believe in God: my Dads Jose Nuñez Vargas and Salvador Federico Nuñez Palenius, my Moms Janette Ann Palenius Alberi and Consuelo Nuñez Solís, my wife Nélida Contreras Sánchez, my son Hector Manuel Nuñez Contreras and my daughter Consuelo Janette Nuñez Contreras. ACKNOWLEDGMENTS This dissertation could not have been completed without the support and help of many people who are gratefully acknowledged here. My greatest debt is to Dr. Daniel James Cantliffe, who has been a dedicated advisor and mentor, but mostly an excellent friend. He provided constant and efficient guidance to my academic work and research projects. This dissertation goal would not be possible without his insightful, invariable and constructive criticism. I thank Dr. Daniel J. Cantliffe for his exceptional course on Advanced Vegetable Production Techniques (HOS-5565) and the economic support for living expenses during my graduate education in UF. I extend my appreciation to my supervisory committee, Dr. Donald J. Huber, Dr. Harry J. Klee, and Dr. Donald Hopkins, for their academic guidance. I am very thankful to Dr. Donald J. Huber for his excellent advice on postharvest guidance, and also for his excellent lectures in Postharvest Physiology (HOS-6331). I am very grateful to Dr. Harry J. Klee for his constant supervision and help on plant molecular issues, as well as for his outstanding teaching in Molecular Biology of Plant Hormones (HOS-5306). I express my gratitude to Dr. Donald Hopkins for his welcoming assistance. I would like to express my deepest appreciation to Dr. Richard Lee and Dr. Charles Niblett for being part of my committee, although they had to leave before this dissertation was completed. I would like to extend an special thank to Dr. Charles Niblett for all his iv help when I arrived at UF, and for being an excellent mentor, but mostly for his exceptional friendship. I extend my appreciation to Dr. Mark Settles for allowing me to use his lab to accomplish some of the plant molecular issues, as well as to Diego Fajardo for his technical advice and untied camaraderie. I would like to make a special acknowledgement to my friends in the Seed Physiology Lab for their help and support during these years: Reggie Salazar, Dr. Do Kim, Dr. Javier Castellanos, Nicole Shaw, Dr. Ivanka Kozareva, Ashwin Paranjpe, Yousef Al-Dlaigan, Juan Carlos Rodriguez, Elio Jovicich, Jennifer Bonina, JeanMarie Mitchell, Teddy McAvoy, Jimmy Webb, Ji-Young and Cecil Shine III. I would like to thank my friends in the Plant Molecular Lab: Dr. Mark G. Taylor, Dr. Joseph A. Ciardi, Dawn Bies, Stephanie Maruhni, Michele Auldridge, Michele Zeigler, Anna Block, Patricia Moussatche, and especially Dr. Denise M. Tieman and Bryan Kevany for their outstanding technical advice and friendship to accomplish this dissertation. I would like to show appreciation to my friends in the Postharvest Lab: Dr. Steve A. Sargent, James Lee, Muharem Ergun, Yasart Karakurt, Kim Cordasco, Adrian Berry, Brandon Hurr and Daniel Stanley. I would like to express my deepest appreciation to the National Council for Science and Technology (CONACYT-México) and EDUCAFIN (Guanajuato-México) which have supported my scholastic and living expenses during my graduate education in UF. v TABLE OF CONTENTS page ACKNOWLEDGMENTS ................................................................................................. iv LIST OF TABLES............................................................................................................. ix LIST OF FIGURES ........................................................................................................... xi ABSTRACT..................................................................................................................... xiv CHAPTER 1 INTRODUCTION ........................................................................................................1 2 LITERATURE REVIEW.............................................................................................4 2.1. Importance of Melon .............................................................................................4 2.1.1 Introduction ..................................................................................................4 2.1.2 Botany and Origin of Cucumis melo ............................................................5 2.1.3 Classification and Cultivars..........................................................................7 2.1.3.1 Naudin’s categories for Cucumis melo L. are listed below:...............9 2.1.3.2 Guis’s categories: ...............................................................................9 2.1.4 Climateric and Non-climateric Fruits.........................................................11 2.2 Postharvest Physiology of Melon .........................................................................11 2.2.1 Physiological Changes During Ripening....................................................11 2.2.2 Ethylene Production ...................................................................................14 2.2.3 Biochemical Changes During Ripening .....................................................15 2.2.3.1 Introduction ......................................................................................15 2.2.3.2 Carbohydrate Metabolism................................................................16 2.2.3.3 Organic Acids...................................................................................19 2.2.3.4 Volatiles ...........................................................................................20 2.2.3.5 Cell Wall Degradation......................................................................23 2.2.3.6 Pigments...........................................................................................26 2.2.4 Ethylene and Molecular Changes During Ripening...................................28 2.2.4.1 Introduction ......................................................................................28 2.2.4.2 Biosynthesis, Perception and Effects of Ethylene............................28 2.3 Melon Biotechnology ...........................................................................................36 2.3.1 Genetic Improvement .................................................................................36 2.3.1.1 Traditional Breeding ........................................................................36 2.3.1.2 Improvement Through Genetic Engineering....................................37 vi 2.3.1.3 Melon Biotechnology.......................................................................42 2.3.2 In vitro Regeneration..................................................................................43 2.3.2.1 Genetic Control ................................................................................44 2.3.2.2 Polyploidization and Somaclonal Variation.....................................46 2.3.2.3 Vitrification ......................................................................................51 2.3.3 Regeneration by Organogenesis.................................................................53 2.3.3.1 Medium Composition.......................................................................54 2.3.3.2 Environmental Factors .....................................................................60 2.3.4 Regeneration by Somatic Embryogenesis..................................................61 2.3.4.1 Medium Composition.......................................................................65 2.3.4.2 Environmental Factors .....................................................................66 2.3.5 Haploid Plants and Embryo Culture...........................................................67 2.3.6 Genetic Transformation..............................................................................70 2.3.6.1 Improvement of Disease Resistance ................................................76 2.3.6.2 Improvement of Tolerance to Physical Factors................................78 2.3.6.3 Improvement of Postharvest Characteristics....................................78 2.4 ‘Galia’ melon........................................................................................................82 2.4.1 Introduction ................................................................................................82 2.4.2 Botany and Origin ......................................................................................83 2.4.3 Postharvest Physiology...............................................................................86 2.4.4 Genetic Improvement by Conventional Methods and Biotechnology .......89 3 EFFECT OF EXPLANT SOURCE ON REGENERATION AND TRANSFORMATION EFFICIENCY IN ‘GALIA’ MELON (Cucumis melo L.) MALE AND FEMALE PARENTAL LINES ............................................................95 3.1 Introduction...........................................................................................................95 3.2 Materials and
Load more

Recommended publications
  • The Heirloom Gardener's Seed-Saving Primer Seed Saving Is Fun and Interesting
    The Heirloom Gardener's Seed-Saving Primer Seed saving is fun and interesting. It tells the story of human survival, creativity, and community life. Once you learn the basics of saving seeds you can even breed your own variety of crop! Share your interesting seeds and stories with other gardeners and farmers while helping to prevent heirloom varieties from going extinct forever. Contact The Foodshed Project to find out about local seed saving events! 1. Food “as a system”...........................................................................................................................5 2. Why are heirloom seeds important?.................................................................................................6 3. How are plants grouped and named?...............................................................................................8 4. Why is pollination important?... ......................................................................................................11 5. What is a monoecious or a dioecious plant?....................................................................................12 6. How do you know if a plant will cross-breed?.................................................................................14 7. What types of seeds are easiest to save?........................................................................................18 8. What about harvesting and storing seeds?.....................................................................................20 9. What do I need to know
    [Show full text]
  • Correlating Sensory Attributes, Textural Parameters and Volatile Organic
    CORRELATING SENSORY ATTRIBUTES, TEXTURAL PARAMETERS AND VOLATILE ORGANIC COMPOUNDS FOR THE ASSESSMENT OF DISTINCTIVE QUALITY TRAITS OF MELON AND PEACH FRUIT CULTIVARS Tiago Luís Cardoso Ferreira Pinhanços de Bianchi Per citar o enllaçar aquest document: Para citar o enlazar este documento: Use this url to cite or link to this publication: http://hdl.handle.net/10803/671744 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art.
    [Show full text]
  • 2018-01-26 Langual Proposal from Foodex2 – Plants in Facet B
    2018-01-26 LanguaL proposal from FoodEx2 – plants in facet B The following are proposals to update LanguaL Facet B, after having indexed EFSA FoodEx2 Exposure hierarchy 20170919. To these, I have added previously-submitted 2017 proposals based on GS1 that have not (yet) been included in LanguaL facet B. GS1 terms and FoodEx2 terms in the following tables are just given to indicate the origin of the proposal. Comments are given in red. First, some simple additions of terms to the SYNONYM field, to make it easier to find descriptors in the LanguaL Food Product Indexer: descriptor synonyms FoodEx2 term FoodEx2 def WORMWOOD [B3433] Add SYN: artemisia vulgaris LITTLE RADISH [B2960] Add SYN: raphanus sativus BLACK RADISH [B2959] Add SYN: raphanus sativus niger PARSNIP [B1483] Add SYN: pastinaca sativa ARRACACHA [B3439] Add SYN: arracacia xanthorrhiza CHAYOTE [B1730] Add SYN: GS1 10006356 - Squash Squash, Choko, grown from Sechium edule (Choko) choko NEW ZEALAND SPINACH Add SYN: GS1 10006427 - New- Tetragonia tetragonoides Zealand Spinach [B1732] tetragonia tetragonoides JAPANESE MILLET Add : barnyard millet; A000Z Barnyard millet Echinochloa esculenta (A. Braun) H. Scholz, Barnyard millet or Japanese Millet. [B4320] echinochloa esculenta INDIAN LONG PEPPER Add SYN! A019B Long pepper fruit Piper longum [B2956] piper longum EUROPEAN ELDER Modify SYN: [B1403] sambucus spp. (which refers to broader term) Should be sambucus nigra DOG ROSE [B2961] ADD SYN: rosa canina LOOSE LEAF LETTUCE Add SYN: [B2087] lactusa sativa L. var. crispa LOLLO ROSSO [B2088] Add SYN: GS1 10006425 - Lollo Lactuca sativa L. var. crispa Rosso red coral lettuce JAVA APPLE [B3395] Add syn! syzygium samarangense Some existing descriptors would also greatly benefit from updated AI (and synonyms): FoodEx2 FoodEx2 def descriptor AI synonyms term ENDIVE [B1314] Add to AI: A00LD Escaroles There are two main varieties of cultivated C.
    [Show full text]
  • High Tunnel Melon and Watermelon Production
    High Tunnel Melon and Watermelon Production University of Missouri Extension M173 Contents Author Botany 1 Lewis W. Jett, Division of Plant Sciences, University of Missouri-Columbia Cultivar selection 3 Editorial staff Transplant production 4 MU Extension and Agricultural Information Planting in the high tunnel 5 Dale Langford, editor Dennis Murphy, illustrator Row covers 6 On the World Wide Web Soil management and fertilization 6 Find this and other MU Extension publications on the Irrigation 7 Web at http://muextension.missouri.edu Pollination 7 Photographs Pruning 8 Except where noted, photographs are by Lewis W. Jett. Trellising 8 Harvest and yield 9 Marketing 10 Pest management 10 Useful references 14 Melon and watermelon seed sources 15 Sources of high tunnels (hoophouses) 16 For further information, address questions to College of Dr. Lewis W. Jett Agriculture Extension State Vegetable Crops Specialist Food and Natural Division of Plant Sciences Resources University of Missouri Columbia, MO 65211 Copyright 2006 by the University of Missouri Board of Curators E-mail: [email protected] College of Agriculture, Food and Natural Resources High Tunnel Melon and Watermelon Production igh tunnels are low-cost, passive, melo has several botanical subgroups (Table 1). solar greenhouses that use no fossil In the United States, reticulatus and inodorus are Hfuels for heating or venting (Figure commercially grown, while the remaining groups 1). High tunnels can provide many benefits to are grown for niche or local markets. horticulture crop producers: The cantaloupe fruit that most Americans • High tunnels are used to lengthen the are familiar with is not actually a true cantaloupe.
    [Show full text]
  • Reimer Seeds Catalog
    LCTRONICLCTRONIC CATALOGCATALOG Cantaloupes & Melons CA52‐20 ‐ Amarillo Oro Melons CA24‐10 ‐ Ambrosia Melons 100 days. Cucumis melo. Open Pollinated. 86 days. Cucumis melo. (F1) The plant The plant produces good yields of 3 ½ to 5 lb produces high yields of 4 ½ to 5 lb round golden yellow oblong melons and can reach cantaloupes. These eastern type melons 15 lbs. It has a creamy white flesh that is have a terrific extra sweet flavor and peach‐ sweet. A winter‐type melon that is a good colored flesh. It has a nectarous aroma and shipper. An excellent choice for home is very juicy. Melons have small seed gardens and market growers. A pre‐1870 cavities. Ambrosia is recognized as one of heirloom variety from Spain. the best‐tasting melons. An excellent choice for home gardens and market growers. Disease Resistant: DM, PM. CA48‐20 ‐ Amish Melons CA31‐20 ‐ Casaba Golden Beauty Melons 90 days. Cucumis melo. Open Pollinated. 90 days. Cucumis melo. Open Pollinated. The plant produces high yields of 4 to 7 lb Plant produces good yields of 6 to 8 lb cantaloupes. The sweet orange flesh is very golden cantaloupes with dark green juicy and has a muskmelon flavor. It does mottling. The melon has white flesh that is well in most regions of the United States, very sweet. Stores well. Does well in hot dry even in extreme heat. An excellent choice climates. Excellent choice for home gardens for home gardens. An heirloom variety from and market growers. A heirloom variety the Amish community. dating back to the 1920s.
    [Show full text]
  • Genetic Resources of the Genus Cucumis and Their Morphological Description (English-Czech Version)
    Genetic resources of the genus Cucumis and their morphological description (English-Czech version) E. KŘÍSTKOVÁ1, A. LEBEDA2, V. VINTER2, O. BLAHOUŠEK3 1Research Institute of Crop Production, Praha-Ruzyně, Division of Genetics and Plant Breeding, Department of Gene Bank, Workplace Olomouc, Olomouc-Holice, Czech Republic 2Palacký University, Faculty of Science, Department of Botany, Olomouc-Holice, Czech Republic 3Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany Academy of Sciences of the Czech Republic, Olomouc-Holice, Czech Republic ABSTRACT: Czech collections of Cucumis spp. genetic resources includes 895 accessions of cultivated C. sativus and C. melo species and 89 accessions of wild species. Knowledge of their morphological and biological features and a correct taxonomical ranging serve a base for successful use of germplasm in modern breeding. List of morphological descriptors consists of 65 descriptors and 20 of them are elucidated by figures. It provides a tool for Cucumis species determination and characterization and for a discrimination of an infraspecific variation. Obtained data can be used for description of genetic resources and also for research purposes. Keywords: Cucurbitaceae; cucumber; melon; germplasm; data; descriptors; infraspecific variation; Cucumis spp.; wild Cucumis species Collections of Cucumis genetic resources include pollen grains and ovules, there are clear relation of this not only cultivated species C. sativus (cucumbers) taxon with the order Passiflorales (NOVÁK 1961). Based and C. melo (melons) but also wild Cucumis species. on latest knowledge of cytology, cytogenetics, phyto- Knowledge of their morphological and biological fea- chemistry and molecular genetics (PERL-TREVES et al. tures and a correct taxonomical ranging serve a base for 1985; RAAMSDONK et al.
    [Show full text]
  • Nutritional Composition and Oil Characteristics of Golden Melon (Cucumis Melo ) Seeds
    Food Science and Quality Management www.iiste.org ISSN 2224-6088 (Paper) ISSN 2225-0557 (Online) Vol.27, 2014 Nutritional Composition and Oil Characteristics of Golden Melon (Cucumis melo ) Seeds Oluwatoyin H. Raji * Oluwaseun T. Orelaja Department of Food Technology, Moshood Abiola Polytechnic Abeokuta, P.O.box 2210 Abeokuta, Ogun state * E-mail of the corresponding author: [email protected] Abstract This study investigated the mineral and proximate composition of Golden/canary melon ( Cucumis melo ) seeds and the physiochemical properties of the seed oil. Proximate composition and physicochemical properties of oil were performed according to AOAC procedures. Minerals were determined using the method of Novozamsky et al. (1983). Results show that the seeds contained high percentage of crude fibre (33.94%) and low percentage of carbohydrate (3.14%). The seeds also contain high value of iron (136.5ppm), zinc (48.35ppm), manganese (25.70ppm), copper (15.40ppm) and low value of calcium (0.023±0.001%). Hexane extracted oil had acid value (2.68mgKOH/g) peroxide value (7.42mgKOH/g), iodine value of (117.43mgKOH/g), saponification value (191.42), free fatty acid (2.34) moisture content (5.68%), and refractive index (1.62) respectively. The seeds serve as good sources of crude fiber, fat and protein. Results also showed that the golden/canary melon oil is non rancid. Keywords: Physicochemical, Golden melon, Hexane extracted oil 1. Introduction Cucurbitaceae (Cucurbit) is an important family comprising one of the most genetically diverse groups of food plants. Most of the plants belonging to this family are frost sensitive and drought-tolerant (Whitaker and Bohn, 1950).
    [Show full text]
  • Replacement of Sodium Chloride by Potassium Chloride in Armenian Cucumber “Cucumis Melo Var
    Middle East Journal of Applied Sciences Volume : 10 | Issue :04 |Oct.-Dec.| 2020 EISSN: 2706 -7947 ISSN: 2077- 4613 Pages: 755-761 DOI: 10.36632/mejas/2020.10.4.66 Replacement of Sodium Chloride by Potassium Chloride in Armenian Cucumber “Cucumis melo Var. Flexuosu” Pickles: Sensory and Microbiological Evaluation Zainab A. Mahdi Biomedical Sciences Department at the School of Arts and Sciences, Lebanese International University, Beirut, Lebanon. E-mail: [email protected] Ali M. Al-Khatib Nutrition and Food Sciences Department at the School of Arts and Sciences, Lebanese International University, Beirut, Lebanon. E-mail: [email protected] Bassam N. Fneich Biological and Chemical Sciences Department at the School of Arts and Sciences, Lebanese International University, Beirut, Lebanon. E-mail: [email protected] Received: 11 Nov. 2020 / Accepted 10 Dec. 2020 / Publication date: 15 Dec. 2020 ABSTRACT Pickles are considered as high salt food mainly due to the presence of high sodium ions added for taste and preservation purposes. This high amount will increase the risk of hypertension and cardiovascular diseases. In this study, an attempt was done to replace Sodium chloride (NaCl) by salt replacer which is potassium chloride (KCl) in Armenian cucumber pickles. Nine treatments were done with different salts percentages with and without chili pepper and garlic. All treatments were incubated at room temperature for 4 weeks then pH measurement, sensory analysis, and microbial analysis were performed. The pH values decreased with incubation and the lowest pH was 3.53±0.03 for Treatment 4 (75% KCl, 25%NaCl). Treatment 4 showed the highest reduction in both total plate count and yeast count with 78.5±3.0×103 CFU/ml and 73.0±3.0×102 CFU/ml respectively.
    [Show full text]
  • Veggies 2018
    Jenny’s Edibles & Blooms VEGGIES [email protected] 2018 Dragon Langerie Bush Bean Maxibel Bush Beans Unique purple stripes mottled over creamy yellow A slender, elegant bean often seen in European- 6-8 inch long flat pods. A delicious conversation style restaurants. They are mouth-watering and piece with super flavor & crispness. Commonly unsurpassed in flavor. Many filets available eaten both as a snap bean and a shell bean. from seed are miniatures, but these dark green stringless beans are a full-sized, 7-inch pod. Maxibel arises on tall, erect plants that require no trellising, and harvest easily. They mature faster than pole beans yielding a huge and concentrated harvest. Romano Bush Bean Fortex Pole Bean This bush-type Romano is the classic flat bean An extraordinary stringless French filet bean with the robust, distinctive full flavor and very growing to over 10 inches long. The delicious heavy yields you would expect from a Romano. round pods have a pronounced nutty bean flavor The plants are compact, and the yield is not only that can be harvested at any size, small and abundant, but starts early, extends over many slender or large and plump. The yields are weeks and will continue to bear until frost. Beans impressive and long lasting. A vigorous climber. are 6 inches long, excellent fresh, and lend themselves equally well to canning and freezing. Romano Pole Bean Golden Grex Beet A personal favorite! This full flavored pole bean is This jewel is a gift to the beet world from breeder early to set pods, and once set, yields a Alan Kapular! Grex, a term from orchid breeding, continuous and glorious harvest of sweet, nutty, alludes to ongoing variation in the gene pool.
    [Show full text]
  • Generate Evaluation Form
    Great Lakes Fruit, Vegetable & Farm Market EXPO Michigan Greenhouse Growers EXPO December 5-7, 2017 DeVos Place Convention Center, Grand Rapids, MI Vine Crops Where: Grand Gallery (main level) Room E & F MI Recertification credits: 2 (1B, COMM CORE, PRIV CORE) OH Recertification credits: 1 (presentations as marked) CCA Credits: PM(1.0) CM(1.0) Moderator: Ben Phillips, Vegetable Extension Educator, MSU Extension, Saginaw, MI 9:00 am Integrated Management of Powdery Mildew and Gummy Stem Blight on Cucurbits: A Florida Perspective (OH: 2B, 0.5 hr) Gary Vallad, Plant Pathology Dept., Univ. of Florida 9:30 am Perspectives and Opportunities for Growing Orange Flesh and Specialty Melons Jonathan Schultheis, Dept. Horticultural Science, North Carolina State University 10:00 am Best Management Practices: Controlling Insects While Protecting Pollinators (OH: CORE, 0.5 hr) Rick Foster, Entomology Dept., Purdue Univ. 10:30 am Cover Cropping in Melons for Reduced Washing After Harvest Chris Gunter, Horticultural Science Dept., North Carolina State Univ. 11:00 am Session Ends Perspectives and Opportunities for Growing Orange Flesh and Specialty Melons Jonathan R. Schultheis North Carolina State University Department of Horticultural Science 2721 Founders Drive, 264 Kilgore Raleigh, NC 27695-7609 [email protected] Orange Flesh Melon Types: The landscape of orange flesh muskmelons (cantaloupes) has changed dramatically over the past five to ten years. Traditionally, there were two primary orange flesh melon types grown and sold; western types, which are fruits that range in size from about 3 to 5 pounds which are heavily netted and not sutured, and eastern types which have some netting, have a shorter shelf life but have a larger fruit size than a western melon, and tend to have a softer flesh with apparent more flavor than a western melon.
    [Show full text]
  • J. Bio. & Env. Sci
    J. Bio. Env. Sci. 2016 Journal of Biodiversity and Environmental Sciences (JBES) ISSN: 2220-6663 (Print) 2222-3045 (Online) Vol. 8, No. 2, p. 166-173, 2016 http://www.innspub.net RESEARCH PAPER OPEN ACCESS Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji Rhimi Awatef1*, Boussaid Mohamed2 1Laboratory of Plant Biotechnology, National Gene Bank of Tunisia (NGBT). Boulevard of the Leader Yasser Arafat, ZI Charguia 1, 1080 Tunis Cedex, Tunisia 2Department of Biology, Laboratory of Plant Biotechnology. National Institute of Applied Sciences and Technologies (INSAT). Centre Urbain Nord, B.P. 676, 1080 Tunis Cedex, Tunisia Article published on February 28, 2016 Key words: Buds; caulogenesis; Cucumis melo L. cv. Maazoun and Beji; plant regeneration; tissue culture. Abstract A procedure for the regeneration of melon (Cucumis melo L.) cv. Maazoun and Beji via shoot organogenesis from cotyledon explants is described. Cotyledons explants cultivated on the basal Murashige and Skoog (1962) medium, added with combinations of BAP, NAA and 2,4-D developed adventitious shoots after two weeks. The rates of caulogenesis varied according to the medium hormonal composition and the genotype. The best induction medium for a morphogenic response was MS containing 0.5 mgl-1 NAA and 2 mgl-1 BAP. The highest caulogenesis percentages were 79.16% and 58.33% for Maazoun and Beji cultivars respectively. The mean number of buds per callus was 2.92. Development phases were characterized by histological studies. Some phases of shoot organogenesis were observed in Cucumis melo L. Maazoun and Beji cultivars. The shoots were rooted in MS medium without growth regulators or added NAA (0.5 or 1 mgl-1).
    [Show full text]
  • Histological Study of Organogenesis in Cucumis Melo L. After Genetic Transformation: Why Is It Difficult to Obtain Transgenic Plants? V Chovelon, V
    Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants? V Chovelon, V. Restier, N. Giovinazzo, Catherine Dogimont, J. Aarouf To cite this version: V Chovelon, V. Restier, N. Giovinazzo, Catherine Dogimont, J. Aarouf. Histological study of organo- genesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?. Plant Cell Reports, Springer Verlag, 2011, 30 (11), pp.2001-2011. 10.1007/s00299-011-1108-9. hal- 01332269 HAL Id: hal-01332269 https://hal.archives-ouvertes.fr/hal-01332269 Submitted on 29 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial| 4.0 International License Version définitive du manuscrit publié dans / Final version of the manuscript published in : Plant Cell Reports, 2011, vol 30 (11) :2001-2011 DOI: 10.1007/s00299-011-1108-9 Histological Study of Organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants? V. Chovelon .V. Restier . N. Giovinazzo . C. Dogimont J. Aarrouf INRA Avignon, UR1052, Unité de Génétique et d’Amélioration des Fruits et Légumes, BP 94, 84143 Montfavet Cedex, France e-mail : [email protected] J.
    [Show full text]