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Chelonian Perivitelline Membrane-Bound Sperm Detection: a New Breeding Management Tool
Zoo Biology 35: 95–103 (2016) RESEARCH ARTICLE Chelonian Perivitelline Membrane-Bound Sperm Detection: A New Breeding Management Tool Kaitlin Croyle,1,2 Paul Gibbons,3 Christine Light,3 Eric Goode,3 Barbara Durrant,1 and Thomas Jensen1* 1San Diego Zoo Institute for Conservation Research, Escondido, California 2Department of Biological Sciences, California State University San Marcos, San Marcos, California 3Turtle Conservancy, New York, New York Perivitelline membrane (PVM)-bound sperm detection has recently been incorporated into avian breeding programs to assess egg fertility, confirm successful copulation, and to evaluate male reproductive status and pair compatibility. Due to the similarities between avian and chelonian egg structure and development, and because fertility determination in chelonian eggs lacking embryonic growth is equally challenging, PVM-bound sperm detection may also be a promising tool for the reproductive management of turtles and tortoises. This study is the first to successfully demonstrate the use of PVM-bound sperm detection in chelonian eggs. Recovered membranes were stained with Hoechst 33342 and examined for sperm presence using fluorescence microscopy. Sperm were positively identified for up to 206 days post-oviposition, following storage, diapause, and/or incubation, in 52 opportunistically collected eggs representing 12 species. However, advanced microbial infection frequently hindered the ability to detect membrane-bound sperm. Fertile Centrochelys sulcata, Manouria emys,andStigmochelys pardalis eggs were used to evaluate the impact of incubation and storage on the ability to detect sperm. Storage at À20°C or in formalin were found to be the best methods for egg preservation prior to sperm detection. Additionally, sperm-derived mtDNA was isolated and PCR amplified from Astrochelys radiata, C. -
Helminth Parasites (Trematoda, Cestoda, Nematoda, Acanthocephala) of Herpetofauna from Southeastern Oklahoma: New Host and Geographic Records
125 Helminth Parasites (Trematoda, Cestoda, Nematoda, Acanthocephala) of Herpetofauna from Southeastern Oklahoma: New Host and Geographic Records Chris T. McAllister Science and Mathematics Division, Eastern Oklahoma State College, Idabel, OK 74745 Charles R. Bursey Department of Biology, Pennsylvania State University-Shenango, Sharon, PA 16146 Matthew B. Connior Life Sciences, Northwest Arkansas Community College, Bentonville, AR 72712 Abstract: Between May 2013 and September 2015, two amphibian and eight reptilian species/ subspecies were collected from Atoka (n = 1) and McCurtain (n = 31) counties, Oklahoma, and examined for helminth parasites. Twelve helminths, including a monogenean, six digeneans, a cestode, three nematodes and two acanthocephalans was found to be infecting these hosts. We document nine new host and three new distributional records for these helminths. Although we provide new records, additional surveys are needed for some of the 257 species of amphibians and reptiles of the state, particularly those in the western and panhandle regions who remain to be examined for helminths. ©2015 Oklahoma Academy of Science Introduction Methods In the last two decades, several papers from Between May 2013 and September 2015, our laboratories have appeared in the literature 11 Sequoyah slimy salamander (Plethodon that has helped increase our knowledge of sequoyah), nine Blanchard’s cricket frog the helminth parasites of Oklahoma’s diverse (Acris blanchardii), two eastern cooter herpetofauna (McAllister and Bursey 2004, (Pseudemys concinna concinna), two common 2007, 2012; McAllister et al. 1995, 2002, snapping turtle (Chelydra serpentina), two 2005, 2010, 2011, 2013, 2014a, b, c; Bonett Mississippi mud turtle (Kinosternon subrubrum et al. 2011). However, there still remains a hippocrepis), two western cottonmouth lack of information on helminths of some of (Agkistrodon piscivorus leucostoma), one the 257 species of amphibians and reptiles southern black racer (Coluber constrictor of the state (Sievert and Sievert 2011). -
Summary Report of Freshwater Nonindigenous Aquatic Species in U.S
Summary Report of Freshwater Nonindigenous Aquatic Species in U.S. Fish and Wildlife Service Region 4—An Update April 2013 Prepared by: Pam L. Fuller, Amy J. Benson, and Matthew J. Cannister U.S. Geological Survey Southeast Ecological Science Center Gainesville, Florida Prepared for: U.S. Fish and Wildlife Service Southeast Region Atlanta, Georgia Cover Photos: Silver Carp, Hypophthalmichthys molitrix – Auburn University Giant Applesnail, Pomacea maculata – David Knott Straightedge Crayfish, Procambarus hayi – U.S. Forest Service i Table of Contents Table of Contents ...................................................................................................................................... ii List of Figures ............................................................................................................................................ v List of Tables ............................................................................................................................................ vi INTRODUCTION ............................................................................................................................................. 1 Overview of Region 4 Introductions Since 2000 ....................................................................................... 1 Format of Species Accounts ...................................................................................................................... 2 Explanation of Maps ................................................................................................................................ -
New Distributional Records of Freshwater Turtles
HTTPS://JOURNALS.KU.EDU/REPTILESANDAMPHIBIANSTABLE OF CONTENTS IRCF REPTILES & AMPHIBIANSREPTILES • VOL &15, AMPHIBIANS NO 4 • DEC 2008 • 28(1):146–151189 • APR 2021 IRCF REPTILES & AMPHIBIANS CONSERVATION AND NATURAL HISTORY TABLE OF CONTENTS NewFEATURE Distributional ARTICLES Records of Freshwater . Chasing Bullsnakes (Pituophis catenifer sayi) in Wisconsin: TurtlesOn the Roadfrom to Understanding West-central the Ecology and Conservation of the Midwest’s GiantVeracruz, Serpent ...................... Joshua M. KapferMexico 190 . The Shared History of Treeboas (Corallus grenadensis) and Humans on Grenada: A Hypothetical Excursion ............................................................................................................................Robert W. Henderson 198 Víctor Vásquez-Cruz1, Erasmo Cazares-Hernández2, Arleth Reynoso-Martínez1, Alfonso Kelly-Hernández1, RESEARCH ARTICLESAxel Fuentes-Moreno3, and Felipe A. Lara-Hernández1 . 1PIMVS HerpetarioThe Texas Palancoatl,Horned Lizard Avenida in Central 19 andnúmero Western 5525, Texas Colonia ....................... Nueva Emily Esperanza, Henry, JasonCórdoba, Brewer, Veracruz, Krista Mougey, Mexico and ([email protected] Perry 204 ) 2Instituto Tecnológico. The KnightSuperior Anole de Zongolica.(Anolis equestris Colección) in Florida Científica ITSZ. Km 4, Carretera a la Compañía S/N, Tepetitlanapa, Zongolica, Veracruz. México 3Colegio de Postgraduados, ............................................. Campus Montecillo.Brian J. Carretera Camposano, México-Texcoco Kenneth -
Redescription, Molecular Characterisation and Taxonomic Re-Evaluation of a Unique African Monitor Lizard Haemogregarine Karyolysus Paradoxa (Dias, 1954) N
Cook et al. Parasites & Vectors (2016) 9:347 DOI 10.1186/s13071-016-1600-8 RESEARCH Open Access Redescription, molecular characterisation and taxonomic re-evaluation of a unique African monitor lizard haemogregarine Karyolysus paradoxa (Dias, 1954) n. comb. (Karyolysidae) Courtney A. Cook1*, Edward C. Netherlands1,2† and Nico J. Smit1† Abstract Background: Within the African monitor lizard family Varanidae, two haemogregarine genera have been reported. These comprise five species of Hepatozoon Miller, 1908 and a species of Haemogregarina Danilewsky, 1885. Even though other haemogregarine genera such as Hemolivia Petit, Landau, Baccam & Lainson, 1990 and Karyolysus Labbé, 1894 have been reported parasitising other lizard families, these have not been found infecting the Varanidae. The genus Karyolysus has to date been formally described and named only from lizards of the family Lacertidae and to the authors’ knowledge, this includes only nine species. Molecular characterisation using fragments of the 18S gene has only recently been completed for but two of these species. To date, three Hepatozoon species are known from southern African varanids, one of these Hepatozoon paradoxa (Dias, 1954) shares morphological characteristics alike to species of the family Karyolysidae. Thus, this study aimed to morphologically redescribe and characterise H. paradoxa molecularly, so as to determine its taxonomic placement. Methods: Specimens of Varanus albigularis albigularis Daudin, 1802 (Rock monitor) and Varanus niloticus (Linnaeus in Hasselquist, 1762) (Nile monitor) were collected from the Ndumo Game Reserve, South Africa. Upon capture animals were examined for haematophagous arthropods. Blood was collected, thin blood smears prepared, stained with Giemsa, screened and micrographs of parasites captured. Haemogregarine morphometric data were compared with the data for named haemogregarines of African varanids. -
Red List of Bangladesh 2015
Red List of Bangladesh Volume 1: Summary Chief National Technical Expert Mohammad Ali Reza Khan Technical Coordinator Mohammad Shahad Mahabub Chowdhury IUCN, International Union for Conservation of Nature Bangladesh Country Office 2015 i The designation of geographical entitles in this book and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of IUCN, International Union for Conservation of Nature concerning the legal status of any country, territory, administration, or concerning the delimitation of its frontiers or boundaries. The biodiversity database and views expressed in this publication are not necessarily reflect those of IUCN, Bangladesh Forest Department and The World Bank. This publication has been made possible because of the funding received from The World Bank through Bangladesh Forest Department to implement the subproject entitled ‘Updating Species Red List of Bangladesh’ under the ‘Strengthening Regional Cooperation for Wildlife Protection (SRCWP)’ Project. Published by: IUCN Bangladesh Country Office Copyright: © 2015 Bangladesh Forest Department and IUCN, International Union for Conservation of Nature and Natural Resources Reproduction of this publication for educational or other non-commercial purposes is authorized without prior written permission from the copyright holders, provided the source is fully acknowledged. Reproduction of this publication for resale or other commercial purposes is prohibited without prior written permission of the copyright holders. Citation: Of this volume IUCN Bangladesh. 2015. Red List of Bangladesh Volume 1: Summary. IUCN, International Union for Conservation of Nature, Bangladesh Country Office, Dhaka, Bangladesh, pp. xvi+122. ISBN: 978-984-34-0733-7 Publication Assistant: Sheikh Asaduzzaman Design and Printed by: Progressive Printers Pvt. -
New Verified Nonindigenous Amphibians and Reptiles in Florida Through 2015, with a Summary of Over 152 Years of Introductions
WWW.IRCF.ORG/REPTILESANDAMPHIBIANSJOURNALTABLE OF CONTENTS IRCF REPTILES & IRCF AMPHIBIANS REPTILES • VOL &15, AMPHIBIANS NO 4 • DEC 2008 • 189 23(2):110–143 • AUG 2016 IRCF REPTILES & AMPHIBIANS CONSERVATION AND NATURAL HISTORY TABLE OF CONTENTS INTRODUCED SPECIES FEATURE ARTICLES . Chasing Bullsnakes (Pituophis catenifer sayi) in Wisconsin: New VerifiedOn the Road to Understanding the Nonindigenous Ecology and Conservation of the Midwest’s Giant Serpent ...................... Amphibians Joshua M. Kapfer 190 . The Shared History of Treeboas (Corallus grenadensis) and Humans on Grenada: A Hypothetical Excursion ............................................................................................................................Robert W. Henderson 198 and ReptilesRESEARCH ARTICLES in Florida through 2015, with a . The Texas Horned Lizard in Central and Western Texas ....................... Emily Henry, Jason Brewer, Krista Mougey, and Gad Perry 204 Summary. The Knight Anole of(Anolis equestris over) in Florida 152 Years of Introductions .............................................Brian J. Camposano, Kenneth L. Krysko, Kevin M. Enge, Ellen M. Donlan, and Michael Granatosky 212 1 1 2 3 3 4 Kenneth L. KryskoCONSERVATION, Louis A. Somma ALERT, Dustin C. Smith , Christopher R. Gillette , Daniel Cueva , Joseph A. Wasilewski , 5 6 7 8 9 10 Kevin M. Enge. , Steve A. Johnson , Todd S. Campbell , Jake R. Edwards , Michael R. Rochford , Rhyan Tompkins , World’s Mammals11 in Crisis .............................................................................................................................................................12 -
Chelonian Advisory Group Regional Collection Plan 4Th Edition December 2015
Association of Zoos and Aquariums (AZA) Chelonian Advisory Group Regional Collection Plan 4th Edition December 2015 Editor Chelonian TAG Steering Committee 1 TABLE OF CONTENTS Introduction Mission ...................................................................................................................................... 3 Steering Committee Structure ........................................................................................................... 3 Officers, Steering Committee Members, and Advisors ..................................................................... 4 Taxonomic Scope ............................................................................................................................. 6 Space Analysis Space .......................................................................................................................................... 6 Survey ........................................................................................................................................ 6 Current and Potential Holding Table Results ............................................................................. 8 Species Selection Process Process ..................................................................................................................................... 11 Decision Tree ........................................................................................................................... 13 Decision Tree Results ............................................................................................................. -
STUDBOOK RHINOCLEMMYS Spp. Introduction by Studbook Keeper Harry Rotmans
STUDBOOK RHINOCLEMMYS spp. Introduction by studbook keeper Harry Rotmans Rhinoclemmys pulcherrima incisa (Photo F.Grünewald) Rhinoclemmys melanosterna (Photo F. Grünewald) Rhinoclemmys punctularia (Photo F. Grünewald) Introduction Recently a start has been made with a studbook for Rhinoclemmys spp. An appeal in Trionyx no. 2(3):94, a magazine of the Nederlandse Schildpadden Vereniging (NSV) (Dutch Turtle/Tortoise Society), produced several positive responses, so the minimum requirements for a new ESF-studbook were met. Because the genus Rhinoclemmys consists of several species, the new studbook will be a coordination of several registrations. The word Rhinoclemmys will be conveniently abbreviated to “R” from now on. Why this studbook? Gradually, more R. turtles are imported from Middle- and South- America. You get the impression that it is meant to become a relatively cheap alternative to protected turtle species from other parts of the world, which are illegal to trade. The clutch of eggs of the R. species are relative small compared to the greater part of the other turtle species (HOFSTRA), mostly 1 or 2 eggs. A number of R. species produces very few clutches in a year. Sooner or later this will lead to the threatening of the population in the wild. Further factors are the affection and destruction of their environment and the pet trade. R. turtles will be used as food in some places by the locals, the same situation as in Asia. Only the population of people in Asia is more dense than in Middle- and South-America, so the need for food is much higher there. Import in the future will probably not become massive, as far as we can see, because there are no farms in the countries concerned with breeding the species. -
Rhinoclemmys Pulcherrima (Gray, 1855) – Ornate Wood Turtle – Lisa Weiss Copyright © 2003 World Chelonian Trust
Rhinoclemmys pulcherrima (Gray, 1855) – Ornate Wood Turtle – Lisa Weiss Copyright © 2003 World Chelonian Trust. All rights reserved Rhinoclemmys pulcherrima incisa - Honduran Wood Turtle Rhinoclemmys pulcherrima manni – Painted Wood Turtle Rhinoclemmys pulcherrima rogerbarbouri - Western Mexican Wood Turtle Rhinoclemmys pulcherrima pulcherrima - Guerrero Wood Turtle INTRODUCTION: Considered to be one of the most beautifully patterned of all turtles, Rhinoclemmys pulcherrima has become widely available through the reptile trade in recent years. Often called Central American Wood Turtles or C.A. Ornate Woods, members of this species are easily and cheaply purchased from dealers, breeders and even mass- market pet store chains such as Petco and Petsmart, where they are frequently misidentified and inappropriately housed. Of the four recognized subspecies, only Rhinoclemmys pulcherrima manni and Rhinoclemmys pulcherrima incisa are commonly encountered; Rhinoclemmys pulcherrima rogerbarbouri and Rhinoclemmys pulcherrima pulcherrima are rarely kept in institutional or private collections. DESCRIPTION: Rhinoclemmys pulcherrima is native to the western coast of Mexico and Central America, from Sonora south to Costa Rica. Found in the southernmost part of this range, R. p. manni is the most colorful subspecies. From some areas within their home range, these turtles display brilliant red-and-yellow eyespots on a dark carapace, with vivid alternating bands of color on the underside margin of the carapace, and intricate patterns of red stripes adorning the green head. R. p. incisa are considerably less showy, with carapace coloration varying from olive to brown and the eyespot markings drastically reduced or absent. Both subspecies are of medium size, with a body type intermediate between that of a slider and a box turtle. -
Turtles of the World, 2010 Update: Annotated Checklist of Taxonomy, Synonymy, Distribution, and Conservation Status
Conservation Biology of Freshwater Turtles and Tortoises: A Compilation ProjectTurtles of the IUCN/SSC of the World Tortoise – 2010and Freshwater Checklist Turtle Specialist Group 000.85 A.G.J. Rhodin, P.C.H. Pritchard, P.P. van Dijk, R.A. Saumure, K.A. Buhlmann, J.B. Iverson, and R.A. Mittermeier, Eds. Chelonian Research Monographs (ISSN 1088-7105) No. 5, doi:10.3854/crm.5.000.checklist.v3.2010 © 2010 by Chelonian Research Foundation • Published 14 December 2010 Turtles of the World, 2010 Update: Annotated Checklist of Taxonomy, Synonymy, Distribution, and Conservation Status TUR T LE TAXONOMY WORKING GROUP * *Authorship of this article is by this working group of the IUCN/SSC Tortoise and Freshwater Turtle Specialist Group, which for the purposes of this document consisted of the following contributors: ANDERS G.J. RHODIN 1, PE T ER PAUL VAN DI J K 2, JOHN B. IVERSON 3, AND H. BRADLEY SHAFFER 4 1Chair, IUCN/SSC Tortoise and Freshwater Turtle Specialist Group, Chelonian Research Foundation, 168 Goodrich St., Lunenburg, Massachusetts 01462 USA [[email protected]]; 2Deputy Chair, IUCN/SSC Tortoise and Freshwater Turtle Specialist Group, Conservation International, 2011 Crystal Drive, Suite 500, Arlington, Virginia 22202 USA [[email protected]]; 3Department of Biology, Earlham College, Richmond, Indiana 47374 USA [[email protected]]; 4Department of Evolution and Ecology, University of California, Davis, California 95616 USA [[email protected]] AB S T RAC T . – This is our fourth annual compilation of an annotated checklist of all recognized and named taxa of the world’s modern chelonian fauna, documenting recent changes and controversies in nomenclature, and including all primary synonyms, updated from our previous three checklists (Turtle Taxonomy Working Group [2007b, 2009], Rhodin et al. -
The Global Magnitude and Implications of Legal and Illegal Wildlife Trade in China
The global magnitude and implications of legal and illegal wildlife trade in China Y UNBO J IAO and T IEN M ING L EE Abstract China is one of the largest consumer markets in is a major component of this global commerce. China is the international legal and illegal wildlife trade. An increas- a major supply source (Nijman, ; Petrossian et al., ing demand for wildlife and wildlife products is threaten- ), but since the s has become one of the world’s ing biodiversity, both within China and in other countries largest consumers of wildlife and wildlife products (Zhou where wildlife destined for the Chinese market is being & Jiang, ; UNODC, ), as a result of economic sourced. We analysed official data on legal imports of development and increasing consumer affluence. China’s CITES-listed species in five vertebrate classes (mammals, demand for wildlife appears to continue to expand as its af- reptiles, amphibians, birds and fish), and on enforcement fluent, urban population increases, and the culture of wild- seizures of illegally traded wildlife, during –. This life consumption spreads from the south to other parts of is the first study that collates and analyses publicly available the country (Zhang et al., ; Zhang & Yin, ). data on China’s legal and illegal wildlife trade and considers This demand drives unsustainable and often illegal a broad range of species. Specifically, we estimated the scale harvest and trade, threatening biological diversity within and scope of the legal and illegal wildlife trade, quantified and beyond China’s borders. The Red List of China’s the diversity of species involved, and identified the major Biodiversity (MEE & CAS, ) shows that % of the trading partners, hotspots and routes associated with illegal , Chinese vertebrate species that have been assessed trade.