Kanamycin type II Recombinant Protein Cat. No.: 92-658

Kanamycin kinase type II Recombinant Protein

Specifications

SPECIES: K. pneumoniae

SOURCE SPECIES: E. coli

SEQUENCE: Met1-Phe264

FUSION TAG: Tag Free

TESTED APPLICATIONS:

APPLICATIONS: This recombinant protein can be used for biological assays. For research use only.

PREDICTED MOLECULAR 29 kD WEIGHT:

Properties

Greater than 95% as determined by reducing SDS-PAGE. PURITY: Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

PHYSICAL STATE: Lyophilized

Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4. It is not recommended to BUFFER: reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.

September 29, 2021 1 https://www.prosci-inc.com/kanamycin-kinase-type-ii-recombinant-protein-92-658.html Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks. STORAGE CONDITIONS: Reconstituted protein solution can be stored at 4-7˚C for 2-7 days. Aliquots of reconstituted samples are stable at -20˚C for 3 months.

Additional Info

OFFICIAL SYMBOL: neo

Aminoglycoside 3'-, APH(3')-II, APH(3')II, Kanamycin kinase type II, ALTERNATE NAMES: -kanamycin phosphotransferase type II, neo

ACCESSION NO.: P00552

Background and References

Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying and widely presented in resistant bacteria. These ATP-dependent phosphorylate the 3'-hydroxyl of a variety of BACKGROUND: including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.

ANTIBODIES FOR RESEARCH USE ONLY.

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