Kanamycin kinase type II Recombinant Protein Cat. No.: 92-658 Kanamycin kinase type II Recombinant Protein Specifications SPECIES: K. pneumoniae SOURCE SPECIES: E. coli SEQUENCE: Met1-Phe264 FUSION TAG: Tag Free TESTED APPLICATIONS: APPLICATIONS: This recombinant protein can be used for biological assays. For research use only. PREDICTED MOLECULAR 29 kD WEIGHT: Properties Greater than 95% as determined by reducing SDS-PAGE. PURITY: Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. PHYSICAL STATE: Lyophilized Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4. It is not recommended to BUFFER: reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. September 29, 2021 1 https://www.prosci-inc.com/kanamycin-kinase-type-ii-recombinant-protein-92-658.html Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks. STORAGE CONDITIONS: Reconstituted protein solution can be stored at 4-7˚C for 2-7 days. Aliquots of reconstituted samples are stable at -20˚C for 3 months. Additional Info OFFICIAL SYMBOL: neo Aminoglycoside 3'-phosphotransferase, APH(3')-II, APH(3')II, Kanamycin kinase type II, ALTERNATE NAMES: Neomycin-kanamycin phosphotransferase type II, neo ACCESSION NO.: P00552 Background and References Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides BACKGROUND: including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 2 https://www.prosci-inc.com/kanamycin-kinase-type-ii-recombinant-protein-92-658.html.