Monoclonal Antibody to Kanamycin Kinase Type II - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AM19000PU-N Monoclonal Antibody to Kanamycin kinase type II - Purified

Alternate names: APH(3')-II, Aminoglycoside 3'-phosphotransferase, Neomycin-kanamycin phosphotransferase type II, kan, neo, nptII Quantity: 0.1 mg Background: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents. Uniprot ID: P00552 NCBI: 573 Host / Isotype: Mouse / IgG2a Recommended Isotype AM03096PU-N Controls: Clone: 4B4D1 Immunogen: Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3). Format: State: Liquid purified Ig fraction. Purification: Affinity Chromatography on Protein A Applications: Suitable for use in ELISA (1/10,000) and Western Blot (1/500-1/2,000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Storage: Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Antibodies should not be exposed to prolonged high temperatures. Shelf life: one year from despatch. Product Citations: Purchased from Acris: 1. Ott, S. Untersuchungen zur Genregulation durch den Tumormarker CD133. Thesis 2017. https://edoc.ub.uni-muenchen.de/20476/1/Ott_Sabrina.pdf General Readings: 1. Landis WG et.al Human and ecological Risk Assessment 6(5): 875-899. 2. Stewart CN et.al BioTechniques 29(4): 832-843. 3. Thomson JA et.al Journal of Food Science 66(2): 188-193.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170426 1 / 1