004b_Posters_S25 28-12-2011 14:05 Pagina 25 Journal of Plant Pathology (2011), 93 (4, Supplement), S4.25-S4.62 Edizioni ETS Pisa, 2011 S4.25 CHARACTERIZATION OF A PR-10 PROTEIN FROM VI- tions of specialized cells that coexist within highly structured com- TIS RIPARIA. M. Alessandrini, A. Lovato, L. Bortesi, G. Zoc- munities. Here we report how phenotypic dissociations in B. sub- catelli, S. Zenoni, M. Pezzotti and A. Polverari. Dipartimento di tilis can induce a significant reduction of the secondary metabo- Biotecnologie, Università degli Studi, Strada le Grazie 15, 37134 lites which are associated with antifungal activity. Strain ET-1 ini- Verona, Italy. E-mail:
[email protected] tially has a rough, dense, colony morphology phenotype (R-form) with undulated edges, which dissociated into a S-form (smooth, We have started a project aimed at characterizing the expres- soft, whitish) and into a M-form (slimy, translucent, amoeba- sion profile and protein activity of pathogenesis-related proteins of shaped colony). Correlations between colony forms, spore forma- the PR-10 family in grapevine. According to a previous microarray tion and in-vitro antifungal activity of cell-free supernatants were analysis, the expression of several genes, annotated as similar to studied. Germinal inhibition over 95% of Penicillium digitatum PR-10 proteins, is strongly increased in resistant Vitis riparia and, conidia was induced only by S and R forms when cell-free super- to a lesser extent, in V. vinifera, following infection with Plas- natants were diluted 1:16 and 1:128 respectively. Biosynthesis of mopara viticola. PR-10 proteins are induced by pathogen infection antifungal molecules in broth was significantly reduced when an and abiotic stresses in a number of species; because they often old R-form colony was used for inoculation because it was already show ribonucleolytic activity it has been suggested that they may dissociated in M and S phenotypic cells.