Reduction by the Methylreductase System in Methanobacterium Bryantii WILLIAM B
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The Wolfe Cycle Comes Full Circle
The Wolfe cycle comes full circle Rudolf K. Thauer1 Max Planck Institute for Terrestrial Microbiology, D-35043 Marburg, Germany n 1988, Rouvière and Wolfe (1) H - ΔμNa+ 2 CO2 suggested that methane formation + MFR from H and CO by methanogenic + 2H+ *Fd + H O I 2 2 ox 2 archaea could be a cyclical process. j O = Indirect evidence indicated that the CoB-SH + CoM-SH fi *Fd 2- a rst step, the reduction of CO2 to for- red R mylmethanofuran, was somehow coupled + * H MPT 2 H2 Fdox 4 to the last step, the reduction of the het- h erodisulfide (CoM-S-S-CoB) to coenzyme CoM-S-S-CoB b MFR M (CoM-SH) and coenzyme B (CoB-SH). H Over 2 decades passed until the coupling C 4 10 mechanism was unraveled in 2011: Via g flavin-based electron bifurcation, the re- CoB-SH duction of CoM-S-S-CoB with H provides 2 H+ the reduced ferredoxin (Fig. 1h) required c + Purines for CO2 reduction to formylmethanofuran ΔμNa + H MPT 4 f H O (2) (Fig. 1a). However, one question still 2 remained unanswered: How are the in- termediates replenished that are removed CoM-SH for the biosynthesis of cell components H Methionine d from CO2 (orange arrows in Fig. 1)? This Acetyl-CoA e anaplerotic (replenishing) reaction has F420 F420H2 recently been identified by Lie et al. (3) as F420 F420H2 the sodium motive force-driven reduction H i of ferredoxin with H2 catalyzed by the i energy-converting hydrogenase EhaA-T H2 (green arrow in Fig. -
Annotation Guidelines for Experimental Procedures
Annotation Guidelines for Experimental Procedures Developed By Mohammed Alliheedi Robert Mercer Version 1 April 14th, 2018 1- Introduction and background information What is rhetorical move? A rhetorical move can be defined as a text fragment that conveys a distinct communicative goal, in other words, a sentence that implies an author’s specific purpose to readers. What are the types of rhetorical moves? There are several types of rhetorical moves. However, we are interested in 4 rhetorical moves that are common in the method section of a scientific article that follows the Introduction Methods Results and Discussion (IMRaD) structure. 1- Description of a method: It is concerned with a sentence(s) that describes experimental events (e.g., “Beads with bound proteins were washed six times (for 10 min under rotation at 4°C) with pulldown buffer and proteins harvested in SDS-sample buffer, separated by SDS-PAGE, and analyzed by autoradiography.” (Ester & Uetz, 2008)). 2- Appeal to authority: It is concerned with a sentence(s) that discusses the use of standard methods, protocols, and procedures. There are two types of this move: - A reference to a well-established “standard” method (e.g., the use of a method like “PCR” or “electrophoresis”). - A reference to a method that was previously described in the literature (e.g., “Protein was determined using fluorescamine assay [41].” (Larsen, Frandesn and Treiman, 2001)). 3- Source of materials: It is concerned with a sentence(s) that lists the source of biological materials that are used in the experiment (e.g., “All microalgal strains used in this study are available at the Elizabeth Aidar Microalgae Culture Collection, Department of Marine Biology, Federal Fluminense University, Brazil.” (Larsen, Frandesn and Treiman, 2001)). -
Untargetted Metabolomic Exploration of the Mycobacterium Tuberculosis Stress Response to Cinnamon Essential Oil
biomolecules Article Untargetted Metabolomic Exploration of the Mycobacterium tuberculosis Stress Response to Cinnamon Essential Oil Elwira Sieniawska 1,* , Rafał Sawicki 2 , Joanna Golus 2 and Milen I. Georgiev 3,4 1 Chair and Department of Pharmacognosy, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland 2 Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland; [email protected] (R.S.); [email protected] (J.G.) 3 Group of Plant Cell Biotechnology and Metabolomics, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria; [email protected] 4 Center of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria * Correspondence: [email protected] Received: 6 January 2020; Accepted: 24 February 2020; Published: 26 February 2020 Abstract: The antimycobacterial activity of cinnamaldehyde has already been proven for laboratory strains and for clinical isolates. What is more, cinnamaldehyde was shown to threaten the mycobacterial plasma membrane integrity and to activate the stress response system. Following promising applications of metabolomics in drug discovery and development we aimed to explore the mycobacteria response to cinnamaldehyde within cinnamon essential oil treatment by untargeted liquid chromatography–mass spectrometry. The use of predictive metabolite pathway analysis and description of produced lipids enabled the evaluation of the stress symptoms shown by bacteria. This study suggests that bacteria exposed to cinnamaldehyde could reorganize their outer membrane as a physical barrier against stress factors. They probably lowered cell wall permeability and inner membrane fluidity, and possibly redirected carbon flow to store energy in triacylglycerols. Being a reactive compound, cinnamaldehyde may also contribute to disturbances in bacteria redox homeostasis and detoxification mechanisms. -
Towards Kinetic Modeling of Global Metabolic Networks with Incomplete Experimental Input on Kinetic Parameters
Towards Kinetic Modeling of Global Metabolic Networks with Incomplete Experimental Input on Kinetic Parameters Ping Ao 1,2 , Lik Wee Lee 1, Mary E. Lidstrom 3,4 , Lan Yin 5, and Xiaomei Zhu 6 1 Department of Mechanical Engineering , University of Washington , Seattle , WA 98195, USA 2 Department of Physics , University of Washington , Seattle , WA 98195, USA 3 Department of Microbiology , University of Washington , Seattle , WA 98195, USA 4 Department of Chemical Engineering , University of Washington , Seattle , WA 98195, USA 5 School of Physics , Peking University , 100871 Beijing , PR China 6 GeneMath , 5525 27 th Ave . N .E., Seattle , WA 98105, USA Abstract: This is the first report on a systematic method for constructing a large scale kinetic metabolic model and its initial application to the modeling of central metabolism of Methylobacterium extorquens AM1, a methylotrophic and environmental important bacterium. Its central metabolic network includes formaldehyde metabolism, serine cycle, citric acid cycle, pentose phosphate pathway, gluconeogensis, PHB synthesis and acetyl-CoA conversion pathway, respiration and energy metabolism. Through a systematic and consistent procedure of finding a set of parameters in the physiological range we overcome an outstanding difficulty in large scale kinetic modeling: the requirement for a massive number of enzymatic reaction parameters. We are able to construct the kinetic model based on general biological considerations and incomplete experimental kinetic parameters. Our method consists of -
Recycling of Vitamin B12 and NAD+ Within the Pdu Microcompartment of Salmonella Enterica Shouqiang Cheng Iowa State University
Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2010 Recycling of vitamin B12 and NAD+ within the Pdu microcompartment of Salmonella enterica Shouqiang Cheng Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Biochemistry, Biophysics, and Structural Biology Commons Recommended Citation Cheng, Shouqiang, "Recycling of vitamin B12 and NAD+ within the Pdu microcompartment of Salmonella enterica" (2010). Graduate Theses and Dissertations. 11713. https://lib.dr.iastate.edu/etd/11713 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. + Recycling of vitamin B12 and NAD within the Pdu microcompartment of Salmonella enterica by Shouqiang Cheng A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Biochemistry Program of Study Committee: Thomas A. Bobik, Major Professor Alan DiSpirito Basil Nikolau Reuben Peters Gregory J. Phillips Iowa State University Ames, Iowa 2010 Copyright © Shouqiang Cheng, 2010. All rights reserved. ii Table of contents Abstract............................................................................................................................. -
Methane Utilization in Methylomicrobium Alcaliphilum 20ZR: a Systems Approach Received: 8 September 2017 Ilya R
www.nature.com/scientificreports OPEN Methane utilization in Methylomicrobium alcaliphilum 20ZR: a systems approach Received: 8 September 2017 Ilya R. Akberdin1,2, Merlin Thompson1, Richard Hamilton1, Nalini Desai3, Danny Alexander3, Accepted: 22 January 2018 Calvin A. Henard4, Michael T. Guarnieri4 & Marina G. Kalyuzhnaya1 Published: xx xx xxxx Biological methane utilization, one of the main sinks of the greenhouse gas in nature, represents an attractive platform for production of fuels and value-added chemicals. Despite the progress made in our understanding of the individual parts of methane utilization, our knowledge of how the whole-cell metabolic network is organized and coordinated is limited. Attractive growth and methane-conversion rates, a complete and expert-annotated genome sequence, as well as large enzymatic, 13C-labeling, and transcriptomic datasets make Methylomicrobium alcaliphilum 20ZR an exceptional model system for investigating methane utilization networks. Here we present a comprehensive metabolic framework of methane and methanol utilization in M. alcaliphilum 20ZR. A set of novel metabolic reactions governing carbon distribution across central pathways in methanotrophic bacteria was predicted by in-silico simulations and confrmed by global non-targeted metabolomics and enzymatic evidences. Our data highlight the importance of substitution of ATP-linked steps with PPi-dependent reactions and support the presence of a carbon shunt from acetyl-CoA to the pentose-phosphate pathway and highly branched TCA cycle. The diverged TCA reactions promote balance between anabolic reactions and redox demands. The computational framework of C1-metabolism in methanotrophic bacteria can represent an efcient tool for metabolic engineering or ecosystem modeling. Climate change concerns linked to increasing concentrations of anthropogenic methane have spiked inter- est in biological methane utilization as a novel platform for improving ecological and human well-being1–4. -
Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane
Supporting Information for Manuscript Submitted to Archaea Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane Wei-Yu Chen1, Lucia Kraková2, Jer-Horng Wu1*, Domenico Pangallo2, Lenka Jeszeová2, Bing Liu3, Hidenari Yasui3 , 1Department of Environmental Engineering, National Cheng Kung University, No.1, University Road, East District, Tainan City 701, Taiwan (ROC) 2Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 84551 Bratislava, Slovakia 3Faculty of Environmental Engineering, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu, Kitakyushu, Japan 808-0135 1 Table S1 Annotation of proteins related to the conversion of methylamines by the methanogens in the CMJP sample analyzed in this study. Relative Coverage Peptides Accession Organism gene Annotation abundance (%) (#) (%) A0A0E3SIM3 Methanosarcina barkeri 3 MSBR3_0618 Dimethylamine:corrinoid methyltransferase 17.13 21 1.39 A0A0E3Q5F5 Methanosarcina vacuolata Z-761 MSVAZ_1366 Methanol methyltransferase corrinoid protein 12.94 15 0.28 A0A0E3P0I6 Methanosarcina siciliae T4/M MSSIT_0113 Monomethylamine:corrinoid methyltransferase 23.58 20 0.48 A0A0E3WTL8 Methanosarcina lacustris Z-7289 MSLAZ_3204 Monomethylamine:corrinoid methyltransferase 15.94 9 0.24 A0A0E3KRC1 Methanosarcina thermophila CHTI-55 MSTHC_1357 Monomethylamine:corrinoid methyltransferase 18.12 10 0.22 A0A0E3WT78 Methanosarcina lacustris Z-7289 MSLAZ_2479 Dimethylamine methyltransferase corrinoid protein 25.00 11 0.13 A0A0E3Q3C8 Methanosarcina vacuolata -
Xuejun Yu Dissertation Final
UNIVERSITY OF CALIFORNIA RIVERSIDE Conversion of Carbon Dioxide to Formate by a Formate Dehydrogenase from Cupriavidus necator A Dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in Bioengineering by Xuejun Yu September 2018 Dissertation Committee: Dr. Ashok Mulchandani, Co-Chairperson Dr. Xin Ge, Co-Chairperson Dr. Russ Hille Copyright by Xuejun Yu 2018 The Dissertation of Xuejun Yu is approved: Committee Co-Chairperson Committee Co-Chairperson University of California, Riverside ACKNOWLEDGEMENTS I would like to express sincere appreciation to my advisor, Professor Ashok Mulchandani, for accepting me to be his student when I was suffering. Thank you very much for your delicate guidance, support and encouragement during the past years. You not only guide me on my PhD study, but also help me to be mature on my personality. From bottom of my heart, I feel very lucky to have you as professor. Also, many thanks go to my other committee members. Professor Xin Ge acted as my co-advisor and provided many valuable comments on my molecular cloning work. Professor Russ Hille has also provided insights, encouragements and advices as a member of my committee members. I have learned so many important insights from our meetings and discussions and thank you for bringing me into the molybdenum/tungsten enzyme conference. I am also thankful to the past and current group members, colleagues and friends - Dimitri Niks, Pankaj Ramnani, Feng Tan, Rabeay Hassan, Trupti Terse, Thien-Toan Tran, Claudia Chaves, Jia-wei Tay, Hui Wang, Pham Tung, Hilda Chan, Yingning Gao and Tynan Young. -
The Genome Sequence of Methanohalophilus Mahii SLPT
Hindawi Publishing Corporation Archaea Volume 2010, Article ID 690737, 16 pages doi:10.1155/2010/690737 Research Article TheGenomeSequenceofMethanohalophilus mahii SLPT Reveals Differences in the Energy Metabolism among Members of the Methanosarcinaceae Inhabiting Freshwater and Saline Environments Stefan Spring,1 Carmen Scheuner,1 Alla Lapidus,2 Susan Lucas,2 Tijana Glavina Del Rio,2 Hope Tice,2 Alex Copeland,2 Jan-Fang Cheng,2 Feng Chen,2 Matt Nolan,2 Elizabeth Saunders,2, 3 Sam Pitluck,2 Konstantinos Liolios,2 Natalia Ivanova,2 Konstantinos Mavromatis,2 Athanasios Lykidis,2 Amrita Pati,2 Amy Chen,4 Krishna Palaniappan,4 Miriam Land,2, 5 Loren Hauser,2, 5 Yun-Juan Chang,2, 5 Cynthia D. Jeffries,2, 5 Lynne Goodwin,2, 3 John C. Detter,3 Thomas Brettin,3 Manfred Rohde,6 Markus Goker,¨ 1 Tanja Woyke, 2 Jim Bristow,2 Jonathan A. Eisen,2, 7 Victor Markowitz,4 Philip Hugenholtz,2 Nikos C. Kyrpides,2 and Hans-Peter Klenk1 1 DSMZ—German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany 2 DOE Joint Genome Institute, Walnut Creek, CA 94598-1632, USA 3 Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM 87545-001, USA 4 Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA 5 Oak Ridge National Laboratory, Oak Ridge, TN 37830-8026, USA 6 HZI—Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany 7 Davis Genome Center, University of California, Davis, CA 95817, USA Correspondence should be addressed to Stefan Spring, [email protected] and Hans-Peter Klenk, [email protected] Received 24 August 2010; Accepted 9 November 2010 Academic Editor: Valerie´ de Crecy-Lagard´ Copyright © 2010 Stefan Spring et al. -
Methylotrophic Methanogenesis in Hydraulically Fractured Shales
Methylotrophic Methanogenesis in Hydraulically Fractured Shales Master’s Thesis Presented in Partial Fulfillment of the Requirements for the Degree Master of Science in the Graduate School of The Ohio State University By: Daniel Nimmer Marcus, B.S. Graduate Program in Microbiology The Ohio State University 2016 Thesis Committee: Kelly Wrighton PhD, Advisor Joseph Krzycki PhD Charles Daniels PhD Matthew Sullivan PhD Copyright by Daniel Nimmer Marcus 2016 ABSTRACT Over the last decade shale gas obtained from hydraulic fracturing of deep shale formations has become a sizeable component of the US energy portfolio. There is a growing body of evidence indicating that methanogenic archaea are both present and active in hydraulically fractured shales. However, little is known about the genomic architecture of shale derived methanogens. Here we leveraged natural gas extraction activities in the Appalachian region to gain access to fluid samples from two geographically and geologically distinct shale formations. Samples were collected over a time series from both shales for a period of greater than eleven months. Using assembly based metagenomics, two methanogen genomes from the genus Methanohalophilus were recovered and estimated to be near complete (97.1 and 100%) by 104 archaeal single copy genes. Additionally, a Methanohalophilus isolate was obtained which yielded a genome estimated to be 100% complete by the same metric. Based on metabolic reconstruction, it is inferred that these organisms utilize C-1 methyl substrates for methanogenesis. The ability to utilize monomethylamine, dimethylamine and methanol was experimentally confirmed with the Methanohalophilus isolate. In situ concentrations of C-1 methyl substrates, osmoprotectants, and Cl- were measured in parallel with estimates of community membership. -
Selenoproteins and Selenoproteomes
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Vadim Gladyshev Publications Biochemistry, Department of June 2006 Selenoproteins and selenoproteomes Vadim N. Gladyshev University of Nebraska-Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/biochemgladyshev Part of the Biochemistry, Biophysics, and Structural Biology Commons Gladyshev, Vadim N., "Selenoproteins and selenoproteomes" (2006). Vadim Gladyshev Publications. 36. https://digitalcommons.unl.edu/biochemgladyshev/36 This Article is brought to you for free and open access by the Biochemistry, Department of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Vadim Gladyshev Publications by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Published in Selenium: Its molecular biology and role in human health, Second Edition, edited by Dolph L. Hatfi eld, Marla J. Berry, and Vadim N. Gladyshev. Springer Science+Business Me- dia LLC, 2006. Pages 99–114. Copyright © 2006 Springer Science+Business Media LLC. Used by permission. C HAPTER 9 Selenoproteins and selenoproteomes Vadim N. Gladyshev Department of Biochemistry, University of Nebraska–Lincoln Summary: In the past several years, progress in genome sequencing and development of specialized bioinformatics tools allowed effi cient identifi cation of selenocysteine-con- taining proteins encoded in completely sequenced genomes. Information is currently available on selenoproteomes from a variety of organisms, including humans, which contain 25 known selenoprotein genes. This review provides basic information about mammalian selenoproteins and other known selenoprotein families. Analysis of full sets of selenoproteins in organisms provides exciting avenues for examining selenoprotein evolution and dependence of organisms on the trace element selenium and allows link- ing selenoproteins with specifi c biological and biomedical effects of dietary selenium. -
An Unconventional Pathway for Reduction of CO2 to Methane in CO-Grown Methanosarcina Acetivorans Revealed by Proteomics
An unconventional pathway for reduction of CO2 to methane in CO-grown Methanosarcina acetivorans revealed by proteomics Daniel J. Lessner*, Lingyun Li†, Qingbo Li*‡, Tomas Rejtar†, Victor P. Andreev†, Matthew Reichlen*, Kevin Hill*, James J. Moran§, Barry L. Karger†¶, and James G. Ferry*¶ *Department of Biochemistry and Molecular Biology and Center for Microbial Structural Biology, Pennsylvania State University, 205 South Frear Laboratory, University Park, PA 16802; †Barnett Institute and Department of Chemistry, Northeastern University, Boston, MA 02115; and §Department of Geosciences and Penn State Astrobiology Research Center, Pennsylvania State University, 220 Deike Building, University Park, PA 16802 Communicated by Lonnie O. Ingram, University of Florida, Gainesville, FL, October 5, 2006 (received for review June 27, 2006) Methanosarcina acetivorans produces acetate, formate, and methane synthesis during growth on CO (16, 22, 23). M. acetivorans was when cultured with CO as the growth substrate [Rother M, Metcalf isolated from marine sediments where giant kelp is decomposed to WW (2004) Proc Natl Acad Sci USA 101:16929–16934], which suggests methane (24). The flotation bladders of kelp contain CO that is a novel features of CO metabolism. Here we present a genome-wide presumed substrate for M. acetivorans in nature. M. acetivorans proteomic approach to identify and quantify proteins differentially produces acetate and formate in addition to methane during abundant in response to growth on CO versus methanol or acetate. CO-dependent growth (17), the first report of any product in The results indicate that oxidation of CO to CO2 supplies electrons for addition to methane and CO2 during growth of methane-producing reduction of CO2 to a methyl group by steps and enzymes of the species.