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The Gut Microbiota of the Egyptian Mongoose As an Early Warning Indicator of Ecosystem Health in Portugal
International Journal of Environmental Research and Public Health Article The Gut Microbiota of the Egyptian Mongoose as an Early Warning Indicator of Ecosystem Health in Portugal Mónica V. Cunha 1,2,3,* , Teresa Albuquerque 1, Patrícia Themudo 1, Carlos Fonseca 4, Victor Bandeira 4 and Luís M. Rosalino 2,4 1 National Institute for Agrarian and Veterinary Research (INIAV, IP), Wildlife, Hunting and Biodiversity R&D Unit, 2780-157 Oeiras, Portugal; [email protected] (T.A.); [email protected] (P.T.) 2 Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, Portugal; [email protected] 3 Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, Portugal 4 Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal; [email protected] (C.F.); [email protected] (V.B.) * Correspondence: [email protected]; Tel.: +351-214-403-500 Received: 1 April 2020; Accepted: 27 April 2020; Published: 29 April 2020 Abstract: The Egyptian mongoose is a carnivore mammal species that in the last decades experienced a tremendous expansion in Iberia, particularly in Portugal, mainly due to its remarkable ecological plasticity in response to land-use changes. However, this species may have a disruptive role on native communities in areas where it has recently arrived due to predation and the potential introduction of novel pathogens. We report reference information on the cultivable gut microbial landscape of widely distributed Egyptian mongoose populations (Herpestes ichneumon, n = 53) and related antimicrobial tolerance across environmental gradients. -
Microbiology Media - Ready to Use, Prepared Plates
Microbiology Media - Ready to use, prepared plates Ready poured plates, general purpose media Ready poured plate, general anaerobe agar 65 65 Columbia blood agar with neomycin. Chromogenic UTI medium Catalogue No Plate diameter, mm Pack qty Price Catalogue No Pack qty Price PO794A 90 10 12.55 PO219A 10 7.54 CLED medium Catalogue No Plate diameter, mm Pack qty Price Ready poured plate, Lactobacilli PO120A 90 10 5.75 64 CLED square plate Catalogue No Plate dimensions, mm Pack qty Price MRS Agar. OXPO0299L 120 x 120 10 16.92 CLED medium with andrades Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO231A 90 10 7.30 PO121A 90 10 5.75 Columbia agar base Ready poured plates, Legionnella media Catalogue No Plate diameter, mm Pack qty Price 65 OXPO0537A 90 10 5.45 MacConkey agar with salt Legionella growth medium, BCYE Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO149A 90 10 5.95 PO5072A 90 10 14.72 MacConkey agar without salt Legionella selective medium, BMPA Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO148A 90 10 5.58 PO0324A 90 10 20.31 MacConkey agar No. 3 Catalogue No Plate diameter, mm Pack qty Price PO495A 90 10 5.90 Malt extract agar Catalogue No Plate diameter, mm Pack qty Price PO182A 90 10 6.38 MRSA agar Catalogue No Plate diameter, mm Pack qty Price OXPO1162A 90 10 15.49 Nutrient agar Catalogue No Plate diameter, mm Pack qty Price PO155A 90 10 5.75 Plate count agar Catalogue No Plate diameter, mm Pack qty Price PO158A 90 10 6.36 R2A agar Catalogue No Plate diameter, mm Pack qty Price PO659A 90 10 6.50 Sabouraud dextrose agar Catalogue No Plate diameter, mm Pack qty Price OXPO0160A 90 10 5.54 Sorbitol MacConkey agar Catalogue No Plate diameter, mm Pack qty Price PO232A 90 10 5.84 Tryptone soya agar Catalogue No Plate diameter, mm Pack qty Price PO163A 90 10 5.49 OXPO0193-C 55 10 10.91 Yeast extract agar Catalogue No Plate diameter, mm Pack qty Price PO441A 90 10 5.08 55. -
Microbiology (Cbcs Structure)
B.Sc. (HONOURS) MICROBIOLOGY (CBCS STRUCTURE) Proposed Scheme for Choice Based Credit System in B.Sc. Honours in Microbiology Year Semester Core Course Ability Skill enhancement Discipline Specific Generic (14 Papers) enhancement course (SEC)(any 2 Elective Course Elective Course 6 credits compulsory papers) (2 Credits (any 4 papers)(6 (any 4 papers) each course each) credits each (6 credits each) (AECC)(2 papers) (2 credits each) I Paper 1 AECC-1 GE-1/2 Paper 2 Paper 1/2 1 (Any one) II Paper 3 AECC-2 GE-1/2 Paper 3/4 Paper 4 (Any one) III Paper 5 SEC-Paper 1/2 GE-1/2 Paper 6 (Any one) Paper 1/2 2 Paper 7 (Any one) IV Paper 8 SEC-Paper 3/4 GE-1/2 Paper 9 (Any one) Paper 3/4 Paper 10 (Any one) V Paper 11 DSE- Paper 1/2(Any one) Paper 12 DSE- Paper 3/4 (Any one) 3 VI Paper 13 DSE- Paper 5/6 Paper 14 (Any one) DSE- Paper 7/8 (Any one) UNIVERSITY OF NORTH BENGAL Page 1 B.Sc. (HONOURS) MICROBIOLOGY (CBCS STRUCTURE) Overall distribution of credits and marks in B.Sc.(Hons.) In Microbiology Course Total Credits /per Total papers Theory Practical Credits I.Core 14 4 2 14X6=84 Courses II.DSE 4 4 2 4X6=24 III.GE 4 4 2 4X6=24 IV.AECC 2 2 - 2x2=4 V.SEC 2 2 - 2x2=4 Grand 140 total UNIVERSITY OF NORTH BENGAL Page 2 B.Sc. (HONOURS) MICROBIOLOGY (CBCS STRUCTURE) Structure of B. -
XL Agar Base • XLD Agar
XL Agar Base • XLD Agar clinical evaluations have supported the claim for the relatively Intended Use high efficiency of XLD Agar in the primary isolation ofShigella XL (Xylose Lysine) Agar Base is used for the isolation and and Salmonella.5-9 differentiation of enteric pathogens and, when supplemented with appropriate additives, as a base for selective enteric media. XLD Agar is a selective and differential medium used for the isolation and differentiation of enteric pathogens from clinical XLD Agar is the complete Xylose Lysine Desoxycholate Agar, specimens.10-12 The value of XLD Agar in the clinical laboratory a moderately selective medium recommended for isolation and is that the medium is more supportive of fastidious enteric organ- differentiation of enteric pathogens, especially Shigella species. isms such as Shigella.12 XLD Agar is also recommended for the XLD Agar meets United States Pharmacopeia (USP), European testing of food, dairy products and water in various industrial Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP)1-3 standard test methods.13-17 General Chapter <62> of the USP performance specifications, where applicable. describes the test method for the isolation of Salmonella from nonsterile pharmaceutical products using XLD Agar as the solid Summary and Explanation culture medium.1 A wide variety of media have been developed to aid in the selective isolation and differentiation of enteric pathogens. Due Principles of the Procedure to the large numbers of different microbial species and strains Xylose is incorporated into the medium because it is fermented with varying nutritional requirements and chemical resistance by practically all enterics except for the shigellae. -
The Safety of Ready-To-Eat Meals Under Different Consumer Handling Conditions
Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere without the permission of the Author. The Safety of Ready-to-Eat Meals Under Different Consumer Handling Conditions A thesis presented in partial fulfilment of the requirements for the degree of Master in Food Technology at Massey University, 0DQDZDWnj, New Zealand Fan Jiang 2016 i Abstract Microbial count is an important index to measure the safety status of a food. This trial aimed to determine the safety of eight meals (four meats and four vegetarians) by using the agar plate counting method to measure the populations of total bacteria and specific pathogenic microorganisms during four day’ abusing. The results showed that chicken & lemon sauce, pork & cranberry loaf and lasagne veg can be considered as acceptable after a series of handling steps including heating and holding in different environments. BBQ beef, quiche golden and pie rice & vegetable were all marginal for the microbial load before heating, but afterwards all of them were acceptable. Casserole chickpea and hot pot sausage were in marginal for the microbial load by the end of trial. Keywords: microbial count; eight meals; pathogenic microorganisms ii Acknowledgements I am like to acknowledge my chief supervisor Prof. Steve Flint for his consistent support and guidance. Without his insightful advice, this project would not go smoothly. I learnt a lot from him how to deal with the challenges faced in the research. I am also grateful to Mrs Julia Good at the Microbiology Laboratory for providing me with her expert guidance on the experimental operation. -
Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment
antibiotics Article Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment Alicia Sorgen 1, James Johnson 2, Kevin Lambirth 2, Sandra M. Clinton 3 , Molly Redmond 1 , Anthony Fodor 2 and Cynthia Gibas 2,* 1 Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] (A.S.); [email protected] (M.R.) 2 Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] (J.J.); [email protected] (K.L.); [email protected] (A.F.) 3 Department of Geography & Earth Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] * Correspondence: [email protected]; Tel.: +1-704-687-8378 Abstract: Bacterial resistance to antibiotics is a growing global concern, threatening human and environmental health, particularly among urban populations. Wastewater treatment plants (WWTPs) are thought to be “hotspots” for antibiotic resistance dissemination. The conditions of WWTPs, in conjunction with the persistence of commonly used antibiotics, may favor the selection and transfer of resistance genes among bacterial populations. WWTPs provide an important ecological niche to examine the spread of antibiotic resistance. We used heterotrophic plate count methods to identify Citation: Sorgen, A.; Johnson, J.; phenotypically resistant cultivable portions of these bacterial communities and characterized the Lambirth, K.; Clinton, -
Salmonella Typhimurium Infection Disrupts but Continuous Feeding of Bacillus Based Probiotic Restores Gut Microbiota in Infected Hens Samiullah Khan and Kapil K
Khan and Chousalkar Journal of Animal Science and Biotechnology (2020) 11:29 https://doi.org/10.1186/s40104-020-0433-7 RESEARCH Open Access Salmonella Typhimurium infection disrupts but continuous feeding of Bacillus based probiotic restores gut microbiota in infected hens Samiullah Khan and Kapil K. Chousalkar* Abstract Background: The gut microbiota plays an important role in the colonisation resistance and invasion of pathogens. Salmonella Typhimurium has the potential to establish a niche by displacing the microbiota in the chicken gut causing continuous faecal shedding that can result in contaminated eggs or egg products. In the current study, we investigated the dynamics of gut microbiota in laying chickens during Salmonella Typhimurium infection. The optimisation of the use of an infeed probiotic supplement for restoration of gut microbial balance and reduction of Salmonella Typhimurium load was also investigated. Results: Salmonella infection caused dysbiosis by decreasing (FDR < 0.05) the abundance of microbial genera, such as Blautia, Enorma, Faecalibacterium, Shuttleworthia, Sellimonas, Intestinimonas and Subdoligranulum and increasing the abundance of genera such as Butyricicoccus, Erysipelatoclostridium, Oscillibacter and Flavonifractor. The higher Salmonella Typhimurium load resulted in lower (P < 0.05) abundance of genera such as Lactobacillus, Alistipes, Bifidobacterium, Butyricimonas, Faecalibacterium and Romboutsia suggesting Salmonella driven gut microbiota dysbiosis. Higher Salmonella load led to increased abundance of genera such as Caproiciproducens, Acetanaerobacterium, Akkermansia, Erysipelatoclostridium, Eisenbergiella, EscherichiaShigella and Flavonifractor suggesting a positive interaction of these genera with Salmonella in the displaced gut microbiota. Probiotic supplementation improved the gut microbiota by balancing the abundance of most of the genera displaced by the Salmonella challenge with clearer effects observed with continuous supplementation of the probiotic. -
Get Consistent and Reliable Results
Need help selecting? M Microbiology Free Tech Support 800-323-4340 Cole-Parmer® Dehydrated Culture Media Get consistent and reliable results – Use when ready or dehydrated culture extends shelf life – Individually numbered with certificate of compliance expiration date, and shelf-life guarantee – Quick dissolve for faster processing – For laboratory use only Media, Dehydrated Media, Media Description Size (g) Catalog number Price Agar, bacteriological This clear gel agar is high grade and manufactured using the ice method 500 GH-14200-10 Azide dextrose broth Use for detection of fecal Streptococci in water and sewage 500 GH-14202-00 Baird parker agar Use for the selective isolation of coagulase positive Staphylococci in food 500 GH-14202-01 Blood agar base with low pH Use to cultivate a wide variety of bacteria 500 GH-14202-02 This is used as a differential medium in the isolation and presumptive identification of Bile Esculin agar 500 GH-14202-03 enterococci/group D streptococci Brain heart infusion agar Use to cultivate a wide spectrum of bacteria, yeasts, and molds 500 GH-14200-12 Brain heart infusion broth Use to cultivate fastidious aerobic and anaerobic bacteria 500 GH-14202-05 Brilliant green bile broth A standard methods medium for confirmed and completed tests for coliforms in water 500 GH-14200-14 Buffered peptone water Helps in the recovery of Salmonella from foods after preservation techniques 500 GH-14202-08 Cetrimide agar Use for the selective isolation of Pseudomonas aeruginosa 500 GH-14200-52 This broth is especially suited for environmental sampling where neutralization of the chemical D/E neutralizing Broth 500 GH-14202-11 is important to determining the bactericidal activity Use to isolate E. -
Superficieibacter Electus Gen. Nov., Sp. Nov., an Extended-Spectrum β
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Digital Commons@Becker Washington University School of Medicine Digital Commons@Becker Open Access Publications 2018 Superficieibacter electus gen. nov., sp. nov., an extended-spectrum β-lactamase possessing member of the enterobacteriaceae family, isolated from Intensive Care Unit surfaces Robert F. Potter Washington University School of Medicine in St. Louis Alaric W. D-Souza Washington University School of Medicine in St. Louis Meghan A. Wallace Washington University School of Medicine in St. Louis Angela Shupe Washington University School of Medicine in St. Louis Sanket Patel Washington University School of Medicine in St. Louis See next page for additional authors Follow this and additional works at: https://digitalcommons.wustl.edu/open_access_pubs Recommended Citation Potter, Robert F.; D-Souza, Alaric W.; Wallace, Meghan A.; Shupe, Angela; Patel, Sanket; Gul, Danish; Kwon, Jennie H.; Beatty, Wandy; Andleeb, Saadia; Burnham, Carey-Ann D.; and Dantas, Gautam, ,"Superficieibacter electus gen. nov., sp. nov., an extended- spectrum β-lactamase possessing member of the enterobacteriaceae family, isolated from Intensive Care Unit surfaces." Frontiers in Microbiology.9,. 1629. (2018). https://digitalcommons.wustl.edu/open_access_pubs/7020 This Open Access Publication is brought to you for free and open access by Digital Commons@Becker. It has been accepted for inclusion in Open Access Publications by an authorized administrator of Digital Commons@Becker. For more information, please contact [email protected]. Authors Robert F. Potter, Alaric W. D-Souza, Meghan A. Wallace, Angela Shupe, Sanket Patel, Danish Gul, Jennie H. Kwon, Wandy Beatty, Saadia Andleeb, Carey-Ann D. -
Product List 2008/09
Product List 2008/09 Part of Thermo Fisher Scientific Oxoid Chromogenic Media … Sheer Brilliance™ to reflect this, the names of products are being changed so that the current range now includes: Brilliance Bacillus cereus Agar Brilliance Candida Agar Brilliance E. coli/coliform Agar Brilliance E. coli/coliform Selective Agar Brilliance Enterobacter sakazakii Agar (DFI) Brilliance Listeria Agar Brilliance UTI Agar Brilliance UTI Clarity Agar Brilliance Salmonella Agar The chromogens used in our Brilliance range of chromogenic media ensure that colony colours are vivid, allowing rapid, easy differentiation and presumptive identification of the organism in question. So remember Brilliance – chromogenic media that delivers clearly visible answers on a single culture plate. Contents 1 Culture Media & Supplements 2 Supplementary Reagents 20 Antibiotic Single Supplements 21 Biochemical Reagents 22 Laboratory Preparations & Biological Extracts 22 Veggietones 24 Bagged Media 25 Dip Slides 25 Atmosphere Generation 26 AGS 26 Traditional Systems 27 Food Testing 28 DuPont Qualicon 29 BAX® System, Test and Components 29 Culture Media for the BAX System 30 RiboPrinter® Microbial Characterisation System 31 Lateral Flow System Range 31 Blood Culture 32 Signal 32 Wampole Isolator® 32 Antimicrobial Susceptibility Testing 33 M.I.C.Evaluator Strips 33 aura image 34 AST Accessories 34 Antimicrobial Susceptibility Testing Discs 35 Miscellaneous 38 Sundries 38 Publications 38 Diagnostics 39 Biochemical I.D. 39 Toxin Detection Kits 40 Agglutination Tests 41 Enzyme Immunoassays 42 Immunofluorescence Assays 43 Lateral Flow Assays 44 Environmental Monitoring and Process Simulation 45 Oxoid Air Sampler 45 Environmental Monitoring Swabs 45 Prepared Media 45 Dehydrated Media 45 Process Simulation 46 2 Culture Media and Supplements A wide range of general purpose, enrichment, selective and differential Anaerobe Basal Broth media, diluents and supplements a medium for general growth of anaerobes NB: information provided here is intended only as an aid to purchasing. -
Food Microbiology
Food Microbiology Food Water Dairy Beverage Online Ordering Available Food, Water, Dairy, & Beverage Microbiology Table of Contents 1 Environmental Monitoring Contact Plates 3 Petri Plates 3 Culture Media for Air Sampling 4 Environmental Sampling Boot Swabs 6 Environmental Testing Swabs 8 Surface Sanitizers 8 Hand Sanitation 9 Sample Preparation - Dilution Vials 10 Compact Dry™ 12 HardyCHROM™ Chromogenic Culture Media 15 Prepared Media 24 Agar Plates for Membrane Filtration 26 CRITERION™ Dehydrated Culture Media 28 Pathogen Detection Environmental With Monitoring Contact Plates Baird Parker Agar Friction Lid For the selective isolation and enumeration of coagulase-positive staphylococci (Staphylococcus aureus) on environmental surfaces. HardyCHROM™ ECC 15x60mm contact plate, A chromogenic medium for the detection, 10/pk ................................................................................ 89407-364 differentiation, and enumeration of Escherichia coli and other coliforms from environmental surfaces (E. coli D/E Neutralizing Agar turns blue, coliforms turn red). For the enumeration of environmental organisms. 15x60mm plate contact plate, The media is able to neutralize most antiseptics 10/pk ................................................................................ 89407-354 and disinfectants that may inhibit the growth of environmental organisms. Malt Extract 15x60mm contact plate, Malt Extract is recommended for the cultivation and 10/pk ................................................................................89407-482 -
Prepared Culture Media
PREPARED CULTURE MEDIA 121517SS PREPARED CULTURE MEDIA Made in the USA AnaeroGRO™ DuoPak A 02 Bovine Blood Agar, 5%, with Esculin 13 AnaeroGRO™ DuoPak B 02 Bovine Blood Agar, 5%, with Esculin/ AnaeroGRO™ BBE Agar 03 MacConkey Biplate 13 AnaeroGRO™ BBE/PEA 03 Bovine Selective Strep Agar 13 AnaeroGRO™ Brucella Agar 03 Brucella Agar with 5% Sheep Blood, Hemin, AnaeroGRO™ Campylobacter and Vitamin K 13 Selective Agar 03 Brucella Broth with 15% Glycerol 13 AnaeroGRO™ CCFA 03 Brucella with H and K/LKV Biplate 14 AnaeroGRO™ Egg Yolk Agar, Modified 03 Buffered Peptone Water 14 AnaeroGRO™ LKV Agar 03 Buffered Peptone Water with 1% AnaeroGRO™ PEA 03 Tween® 20 14 AnaeroGRO™ MultiPak A 04 Buffered NaCl Peptone EP, USP 14 AnaeroGRO™ MultiPak B 04 Butterfield’s Phosphate Buffer 14 AnaeroGRO™ Chopped Meat Broth 05 Campy Cefex Agar, Modified 14 AnaeroGRO™ Chopped Meat Campy CVA Agar 14 Carbohydrate Broth 05 Campy FDA Agar 14 AnaeroGRO™ Chopped Meat Campy, Blood Free, Karmali Agar 14 Glucose Broth 05 Cetrimide Select Agar, USP 14 AnaeroGRO™ Thioglycollate with Hemin and CET/MAC/VJ Triplate 14 Vitamin K (H and K), without Indicator 05 CGB Agar for Cryptococcus 14 Anaerobic PEA 08 Chocolate Agar 15 Baird-Parker Agar 08 Chocolate/Martin Lewis with Barney Miller Medium 08 Lincomycin Biplate 15 BBE Agar 08 CompactDry™ SL 16 BBE Agar/PEA Agar 08 CompactDry™ LS 16 BBE/LKV Biplate 09 CompactDry™ TC 17 BCSA 09 CompactDry™ EC 17 BCYE Agar 09 CompactDry™ YMR 17 BCYE Selective Agar with CAV 09 CompactDry™ ETB 17 BCYE Selective Agar with CCVC 09 CompactDry™ YM 17 BCYE