PREPARED CULTURE MEDIA

121517SS PREPARED CULTURE MEDIA

Made in the USA AnaeroGRO™ DuoPak A 02 Bovine Blood Agar, 5%, with Esculin 13 AnaeroGRO™ DuoPak B 02 Bovine Blood Agar, 5%, with Esculin/ AnaeroGRO™ BBE Agar 03 MacConkey Biplate 13 AnaeroGRO™ BBE/PEA 03 Bovine Selective Strep Agar 13 AnaeroGRO™ Agar 03 with 5% Sheep Blood, Hemin, AnaeroGRO™ Campylobacter and Vitamin K 13 Selective Agar 03 Brucella Broth with 15% Glycerol 13 AnaeroGRO™ CCFA 03 Brucella with H and K/LKV Biplate 14 AnaeroGRO™ Egg Yolk Agar, Modified 03 Buffered Peptone Water 14 AnaeroGRO™ LKV Agar 03 Buffered Peptone Water with 1% AnaeroGRO™ PEA 03 Tween® 20 14 AnaeroGRO™ MultiPak A 04 Buffered NaCl Peptone EP, USP 14 AnaeroGRO™ MultiPak B 04 Butterfield’s Phosphate Buffer 14 AnaeroGRO™ Chopped Meat Broth 05 Campy Cefex Agar, Modified 14 AnaeroGRO™ Chopped Meat Campy CVA Agar 14 Broth 05 Campy FDA Agar 14 AnaeroGRO™ Chopped Meat Campy, Blood Free, Karmali Agar 14 Broth 05 Cetrimide Select Agar, USP 14 AnaeroGRO™ Thioglycollate with Hemin and CET/MAC/VJ Triplate 14 Vitamin K (H and K), without Indicator 05 CGB Agar for Cryptococcus 14 Anaerobic PEA 08 15 Baird-Parker Agar 08 Chocolate/Martin Lewis with Barney Miller Medium 08 Lincomycin Biplate 15 BBE Agar 08 CompactDry™ SL 16 BBE Agar/PEA Agar 08 CompactDry™ LS 16 BBE/LKV Biplate 09 CompactDry™ TC 17 BCSA 09 CompactDry™ EC 17 BCYE Agar 09 CompactDry™ YMR 17 BCYE Selective Agar with CAV 09 CompactDry™ ETB 17 BCYE Selective Agar with CCVC 09 CompactDry™ YM 17 BCYE with DGVP 09 CompactDry™ XSA 17 BCYE Selective Agar with GVPC 09 Derm-Duet™ II DTM/RSM Biplate 18 BE Agar 09 Derm-Duet™ RSM/DTM Biplate 18 BE Agar with Azide 09 Dermatophyte Milk Agar 18 BE Agar with Azide/CNA Biplate 09 Agar 18 BG Agar 10 SabDex Agar with C and G/DTM Biplate 18 BG Agar with Novobiocin 10 Dilu-Lok™ Pre-filled Dilution Vials 19 Headquarters BG Bile Broth with Durham Tube 10 Chocolate/MTM Biplate 20 1430 West McCoy Lane BHI Agar 10 Chocolate Agar with Bacitracin 20 Santa Maria, CA 93455 BHI Agar with Blood 10 CIN/MacConkey with Sorbitol Biplate 20 800.266.2222 : phone [email protected] BHI Agar with Blood and 11 CIN Agar 20 www.HardyDiagnostics.com BHI Agar, with Blood, Chloramphenicol, CLED Agar 20 and Gentamicin 11 CNA Agar 20 Distribution Centers BHI Agar with Chloramphenicol and CNA Agar/EMB Agar Biplate 20 Santa Maria, California Cycloheximide 11 CNA Agar/MacConkey Agar Biplate 21 Olympia, Washington Salt Lake City, Utah BHI Agar with Vancomycin 11 Columbia Agar, USP 21 Phoenix, Arizona BHI Broth 11 Columbia Agar, 5% Sheep Blood 21 Dallas, Texas Springboro, Ohio BHI Broth with 5% Fildes Solution 11 Columbia Agar with 5% Sheep Blood/ Lake City, Florida BiGGY Agar 11 MacConkey Agar Biplate 21 Albany, New York Raleigh, North Carolina Blood Agar, 5% 12 Cooked Meat with Iron, H and K 21 Blood Agar, 5%/Chocolate Biplate 12 Corn Meal Agar with Tween® 80 21 Blood Agar 5%/EMB Biplate 12 CT-SMAC 21

The Quality Management Blood Agar, 5%/MacConkey Biplate 12 CTA 22 System at the Hardy Blood Agar, 5% with Ampicillin 12 D/E Neutralizing Broth 22 Diagnostics manufacturing facility is certified to ISO Blood Agar, 8% 13 D/E Neutralizing Agar 22 13485. Blood Agar, 10% 13 DNase Agar with Toluidine Blue 22 Blood Agar, EH 13 Diamonds Medium, Modified 22 Blood, Sheep, Defibrinated 13 Dichloran-Glycerol (DG-18) Agar 22 Bovine Blood Agar, 5% 13 EC Broth with Durham Tube 22 EC Broth with MUG iii22 Copyright © 2018 Hardy Diagnostics EE Broth Mossel, USP 23 Letheen Agar, Modified with MSA with Oxacillin 38 Egg Yolk Agar, Modified 23 Tween® 80, 1.5% 32 Mueller Hinton Agar 38 EMB Levine Agar 23 Letheen Agar/MacConkey Agar Biplate 33 Mueller Hinton Agar with Blood 39 EnteroScreen 4™ 23 Letheen Broth 33 Mueller Hinton Broth 39 EnviroTrans™ Swab Rinse Kit 24 Letheen Broth, Modified 33 Mycobiotic Agar 39 FB Broth 24 LIA 33 Mycoplasma Agar 39 Fluid A, USP 24 Liver Infusion Agar Selective 33 Mycoplasma Broth 39 Fluid D, USP 24 LJ Gruft 33 MycoVue™ Slide Culture System 40 Fluid K, USP 24 LJ Medium 33 MYP Agar 40 Fraser Broth, Modified 24 LJ with Ferric Ammonium Citrate, Slant 33 NaCl (Salt Tolerance) 6.5% Broth 40 FTM with Lecithin and Tween® 25 LJ with Pyruvate 33 Neomycin Anaerobic Blood Agar 40 FTM with Hemin and Vitamin K 25 LKV Agar 34 Neutralizing Buffer 40 GBS Detect™ 25 Loeffler’s Slant 34 Nitrate Broth with Durham Tube 41 GN Broth 25 m EI Agar 34 Non-Nutrient 41 Group A Beta Strep Agar 25 m Endo LES Agar 34 41 Haemophilus ID Quadplate 25 m FC Agar with 1% Rosolic Acid 34 Nutrient Agar with MUG 41 HardyCHROM™ BluEcoli™ Biplate 26 m HPC Agar 35 Nutrient Broth 41 HardyCHROM™ Candida 26 m PA Agar 35 OF Media 41 HardyCHROM™ ECC 26 m TEC Agar, Modified 35 ONPG Rapid Test Broth 41 HardyCHROM™ ESBL 27 MacConkey Agar 35 Orange Serum Agar 41 HardyCHROM™ HUrBi™ 27 MacConkey Agar with Cefotaxime 35 Oxford Medium, Modified 41 HardyCHROM™ 27 MacConkey Agar with Sorbitol 35 Page’s Saline 41 HardyCHROM™ MRSA 27 MacConkey/EMB Biplate 35 PALCAM 41 HardyCHROM™ O157 28 MacConkey Broth 36 PC Agar 41 HardyCHROM™ Sakazakii 28 Malt Extract Agar 36 Phenylalanine Agar Slant 42 HardyCHROM™ 28 Malt Extract Agar with Lecithin and Tween 36 PEA with 5% Sheep Blood 42 HardyCHROM™ Salmonella/XLT-4 Malt Extract Agar with 0.01% Peptic Digest Agar with Fildes Agar Biplate 28 Chloramphenicol 36 and Nafcillin 42 HardyCHROM™ SS NoPRO 28 Martin Lewis with Lincomycin 36 Peptone Water 42 HardyCHROM™ Staph aureus 29 Mastitis Triplate 37 Peptone Salt 42 HardyCHROM™ UTI 29 McFarland Standard 37 Phenol Red Broth with Durham Tube 42 HardyCHROM™ Vibrio 30 37 Phosphate Buffered Saline (PBS), pH 6.8 42 HE Agar 30 Middlebrook 7H11/7H11 Selective Phosphate Buffered Saline (0.01M) with HE Agar/SS Agar Biplate 31 Agar Biplate 37 0.02% Tween® 80 42 HTM Agar 31 37 Phosphate Buffered Saline, pH 7.5 43 Inhibitory Mold Agar 31 Middlebrook 7H11 Selective 37 Phosphate Buffer with Magnesium Inhibitory Mold Agar with Gentamicin 31 Middlebrook 7H11, Thin Pour 37 Chloride 43 Iodine-Iodide Solution 31 37 Phosphate Buffered Saline with 0.05% KF Agar 31 Middlebrook 7H9 Broth with Tween® 80 37 Tween 20 43 KIA Slant 31 MIL Medium 37 PLET Agar 43 Lactobacilli MRS Agar 31 MIO Medium 38 43 Lactobacilli MRS Broth 32 Moeller’s Decarboxylase 38 Potato Dextrose Agar with Lactose Broth 32 Motility Test Medium with TTC 38 Chlortetracycline 43 with Durham Tube 32 Motility Test Medium without TTC 38 Potato Dextrose Agar with Leeds Medium 32 MRSA Screen Plate 38 Chloramphenicol 43 Letheen Agar 32 MR-VP Broth 38 Potato Dextrose Agar with TA 43 Letheeniv Agar, Modified 32 MSA Agar 38 Potato Flake Agar 43 TCBS Agar 51 Urea Medium, Rapid 54 Tergitol 7 Agar with TTC 51 UVM Modified Listeria Broth 54 Tetrathionate Broth 51 V9 Agar 54 Tetrathionate Broth with Brilliant Green 51 Vogel and Johnson Agar 54 Thayer Martin Agar 51 VRBA 54 Thayer Martin Agar, Modified 52 VRBGA 54 Thioglycollate with H and K 52 Wallenstein Media 54 Thioglycollate without Indicator 52 Water with Tween® 80 54 Thioglycollate without Indicator, with XLD Agar 54 Supplements 52 XLT4 Agar 54 Thioglycollate with Indicator 52 XLT4 Agar/Brilliant Green Agar with Todd Hewitt Broth 52 Sulfadiazine Biplate 54 Todd Hewitt Broth with SXT 52 Tryptic Soy Agar (TSA), FlexEM™ Potato Flake Selective Agar 44 Trehalose Broth, Rapid 52 Media Paddles 56 PPLO Selective Agar 44 Tryptic Soy Agar 52 Malt Extract Agar (MEA), FlexEM™ Pseudomonas Agar F 44 TSA, SterEM™, Irradiated, USP 52 Media Paddles 56 Pseudomonas Isolation Agar 44 TSA with Cycloheximide 52 (TSB), USP, R10 Broth 44 TSA with Lecithin and Tween® 80 52 ReadyRack™ 57 Rapid Ornithine 44 TSA Blood/CLED/MAC 53 Fluid Thioglycollate Medium (FTM), USP, Regan-Lowe Agar 45 TSA with Lecithin and Tween® 80, USP 53 ReadyRack™ 57 Regan-Lowe Semisolid 45 TSB, USP, Bottles 53 Rose Agar 45 TSB, Double Strength, USP 53 Rose/MacConkey Biplate 45 TSB Tubes 53 Rose Bengal Agar with Chloramphenicol 45 TSB with 6.5% Sodium Chloride 54 RTF Modified Casman Agar 46 TSB with Lecithin and Tween® 20, USP 54 RV Broth, USP 46 TSB with Lecithin and Tween® 80 54 SabDex Agar 46 TSI Agar 54 SabDex Agar, Emmons 46 Tryptone Broth, 2% 54 SabDex Agar with C and G/DTM Biplate 46 Tryptone Glucose Extract Agar 54 SabDex Agar with Chloramphenicol 46 Urea (Christensen’s Urea) Agar Slant 54 SabDex Agar with Chloramphenicol and Tetracycline 46 SabDex Agar with Gentamicin and Chloramphenicol 46 SabDex Agar with Lecithin and Tween® 80 46 SabDex Agar with Lecithin and Tween® 80, SterEM™, Irradiated 46 SabDex Broth 47 SabHI Agar 47 SabHI Agar with Blood, Chloramphenicol, and Cycloheximide 47 Saline, 0.45% 47 Saline, 0.85% 47 Saline, 0.9% with 0.5% Tween® 80 48 Selective Beta Strep Agar, Group A 48 Selective Strep Agar (COBA Medium) 48 Selenite Cystine Broth 48 SIM Medium 48 Simmons Citrate Agar 49 Skim Milk Agar 49 SP4 Agar 49 SP4 Broth 49 SS Agar 49 Standard Methods Agar 49 Sterile Deionized Water 49 Sterile Water 49 Strep B Carrot Broth™ One-Step 50 TAT Broth 51 TB Base Digestant 51 v SELECTION Since 1980, Hardy Diagnostics has grown to manufacture an extensive selection of prepared media products which are PREPARED packaged in plates, tubes, bottles, bags, and flasks. MEDIA QUALITY CONTROL PROGRAM Hardy products go through rigorous and extensive QC testing and lot specific Certificates of Analysis are available at HardyDiagnostics.com.

GUARANTEED DATING We provide at least one month of shelf life on most products.

TECHNICAL ASSISTANCE Instructions for Use (IFU) are available for all products through our web catalog. Experienced Medical Technologists and microbiologists are available for personalized assistance.

SAME DAY SHIPPING Orders placed before 1:30pm PST are processed and shipped out the same day. Most of our customers receive their orders the following morning.

Call us at 800.266.2222

For over 37 years Hardy Diagnostics has worked with its laboratory partners to manufacture custom culture media. Whether for specialized formulations, containers, or packaging, Hardy Diagnostics is ready to assist you with your specific needs.

The Quality Management System at Hardy Diagnostics is ISO 13485 certified and is licensed by the FDA as an “In vitro Medical Device Manufacturer,” which ensures the highest standards of quality for our customers.

vi Easy-to-open Bag is flushed with Oxygen Scavenger Sachet

Pre-scored Notch Oxygen-free Gas Absorbs residual O2 after gas flushing.

3 plates/pack 15x100mm

Oxygen Impermeable Foil Pouch Moisture Absorbing Desiccant

AnaeroGRO™ plates are pre-reduced and packaged in an oxygen barrier mylar pouch that has been flushed with oxygen-free gas. In addition, each pouch contains an oxygen scavenger sachet and a desiccant to control excess condensation. ¨¨Pre-reduced (ready-to-use) culture media ¨¨Room temperature storage packaged in oxygen-free, gas-flushed foil ¨¨Quality Control tested for reliable and pouches reproducible results ¨¨Contains an oxygen scavenger sachet and a ¨¨Exceptional growth and performance moisture absorbing desiccant packet characteristics compared to other brands ¨¨Foil pouches with easy-to-open, pre-scored ¨¨Manufactured in an ISO 13485 certified facility notch ¨¨Competitive pricing, quality products, and ¨¨Wide variety of packaging combinations exceptional service and support available: monoplates, biplates, and primary set-up combinations

¨¨See the Instructions For Use (IFU) online for the complete procedure with color photos. ¨¨ t Remember that Hardy offers all your anaerobic te a bacteriology supplies: si m »»Gas generators eb .co »»Jars w tics »»Pouches ur os »»Indicators o n »»ID reagents and disks it ag is Di V dy ar H 01 AnaeroGRO™ DuoPak A COMBINATION (2 plates per pouch) ¨¨Brucella with Hemin and Vitamin K Monoplate PACKS ¨¨BBE/LKV Biplate For the cultivation and differentiation of anaerobic Monoplate: Brucella with Hemin and Vitamin K Monoplate Recommended for use in the primary isolation, quantitation, and partial identification of obligately anaerobic . Biplate: BBE/LKV Section I: BBE Agar is an enriched, selective, and differential medium recommended for the isolation and presumptive identification of obligately anaerobic Gram-negative bacilli of the Bacteroides fragilis group and Bilophila spp. Section II: LKV Agar is recommended for use in the selective isolation and partial identification of obligately an- aerobic Gram-negative bacilli, such as Prevotella spp. and Bacteroides spp. 15x100mm plate, 2 plates/mylar pouch AG302

AnaeroGRO™ DuoPak B (2 plates per pouch) ¨¨Brucella with Hemin and Vitamin K Monoplate ¨¨BBE/PEA Biplate Bacteroides fragilis growing on Anaerobic For the cultivation and differentiation of PEA, Cat. no. A90. anaerobic bacteria Monoplate: Brucella with Hemin and Vitamin K Recommended for use in the primary isolation, quantitation, and partial identification of obligately anaerobic microorganisms. Biplate: BBE/PEA Section I: BBE Agar is an enriched, selective, and differential medium recommended for the isolation and presumptive identification of obligately anaerobic Gram-negative bacilli of the Bacteroides fragilis group and Bilophila spp. Section II: Recommended for use as an enriched and selective medium for the cultivation and selective isolation of Gram-positive and negative obligate anaerobic bacteria. 15x100mm plate, 2 plates/mylar pouch AG312 02 PRE-REDUCED PLATES

AnaeroGRO™ BBE Agar AnaeroGRO™ Brucella Agar AnaeroGRO™ Egg Yolk Agar, (Bacteroides Bile Esculin) (Brucella with Hemin and Vitamin K, Modified An enriched, selective, and Anaerobe) An enriched, non-selective, differential medium recom- For use in the primary differential medium mended for the isolation isolation, quantitation, and recommended for use in the and presumptive identifica- identification of obligately detection of lecithinase and tion of obligately anaerobic anaerobic microorganisms. lipase production; proteolytic Gram-negative bacilli of the The medium is also suitable activity of certain obligate Bacteroides fragilis group for the growth of aerobic anaerobes; and, presumptive and Bilophila spp. and microaerophilic bacteria identification of various 15x100mm plate, when incubated under the , Fusobacterium, 1 plate/mylar pouch AG051 appropriate conditions. and Prevotella spp. Modified 15x100mm plate: Egg Yolk Agar is also used AnaeroGRO™ BBE/LKV 1 plate/mylar pouch AG301 in the Nagler Test for the (Bacteroides /Brucella 4 plates/mylar pouch AG304 presumptive identification of Agar with Laked Sheep Blood, Kanamycin, AnaeroGRO™ Campylobacter . 15x100mm plate, and Vancomycin) Selective Agar Section I: BBE Agar is an 1 plate/mylar pouch AG401 Recommended for the se- enriched, selective, and dif- lective isolation of Campy- ferential medium recom- AnaeroGRO™ LKV Agar lobacter jejuni subsp. jejuni, mended for the isolation (Laked Blood with Kanamycin and growth of normal fecal flora and presumptive identifi- Vancomycin) is inhibited on this medium. cation of obligately anaer- Recommended for use 15x100mm plate, obic Gram-negative bacilli in the selective isolation 1 plate/mylar pouch AG701 of the Bacteroides fragilis and partial identification group and Bilophila spp. AnaeroGRO™ CCFA of obligately anaerobic Section II: LKV Agar is recom- (Cycloserine-Cefoxitin Fructose Agar) Gram-negative bacilli, mended for use in the se- An enriched selective such as Prevotella spp. and lective isolation and partial and differential medium Bacteroides spp. identification of obligately recommended for the 15x100mm plate, anaerobic Gram-negative cultivation and isolation 1 plate/mylar pouch AG601 bacilli, such as Prevotella of Clostridium difficile, AnaeroGRO™ PEA spp. and Bacteroides spp. a recognized cause of (Phenylethyl Alcohol Agar) 15x100mm biplate, intestinal infections and For use as an enriched and 1 plate/mylar pouch AG061 Pseudomembranous colitis selective medium for the following antibiotic therapy. cultivation and selective iso- 15x100mm plate, lation of Gram-positive and 1 plate/mylar pouch AG501 negative obligate anaerobic bacteria. It is useful in isolat- ing obligate anaerobes from mixed flora by inhibiting Gram-negative facultative anaerobes and controls for swarming organisms. 15x100mm plate, 1 plate/mylar pouch AG901

03 PRE-REDUCED PLATES

AnaeroGRO™ MultiPak A AnaeroGRO™ MultiPak B (3 plates per pouch) (3 plates per pouch) ¨¨Brucella with Hemin and Vitamin K Monoplate ¨¨Brucella with Hemin and Vitamin K Monoplate ¨¨Phenylethyl Alcohol Monoplate ¨¨Laked Blood with Kanamycin and Vancomycin Monoplate ¨¨Bacteroides Bile Esculin Agar/Laked Blood with Kanamycin ¨¨Phenylethyl Alcohol Monoplate Vancomycin Biplate Monoplate: AnaeroGRO™ Brucella Agar with Monoplate: AnaeroGRO™ Brucella Agar with Hemin and Vitamin K Recommended Hemin and Vitamin K Recommended for use in the primary isolation, for use in the primary isolation, quantitation, and partial quantitation, and partial identification of obligately anaerobic identification of obligately anaerobic microorganisms from clinical microorganisms from clinical specimens. The medium is also specimens. The medium is also suitable for the growth of aerobic suitable for the growth of aerobic and microaerophilic bacteria when and microaerophilic bacteria when incubated under the incubated under the appropriate conditions. appropriate conditions. Monoplate: AnaeroGRO™ LKV Agar Monoplate: AnaeroGRO™ Anaerobic PEA Agar Recommended for use in the selective Recommended for use as an enriched isolation and partial identification of and selective medium for the obligately anaerobic Gram-negative cultivation and selective isolation of bacilli, such as Prevotella spp. and Gram-positive and negative obligate Bacteroides spp. anaerobic bacteria. It is useful in isolating obligate anaerobes from Monoplate: AnaeroGRO™ Anaerobic PEA mixed flora, by inhibiting Gram- Recommended for use as an enriched negative facultative anaerobes and the and selective medium for the control of swarming organisms. cultivation and selective isolation of Biplate: AnaeroGRO™ BBE/LKV Gram-positive and negative obligate Section I: AnaeroGRO™ BBE Agar is an anaerobic bacteria. It is useful in enriched, selective, and differential isolating obligate anaerobes from medium recommended for the mixed flora, by inhibiting Gram- rapid isolation and presumptive negative facultative anaerobes and the identification of obligately anaerobic control of swarming organisms. Gram-negative bacilli of the 15x100mm plate, Bacteroides fragilis group 3 plates/mylar pouch AG313 Bilophila spp. Section II: AnaeroGRO™ LKV (Laked Blood with Kanamycin and Vancomycin) Agar is recommended for use in the selective isolation and partial identification of obligately anaerobic Gram-negative bacilli, such as Prevotella spp. and Bacteroides spp. 15x100mm plate, 3 plates/mylar pouch AG303

04 TUBES

AnaeroGRO™ Chopped Meat Broth Recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium spp. 16x125mm glass tube, needle port hungate cap, 9ml fill, Needle port 20/pk AG21H (Hungate Cap) AnaeroGRO™ Chopped Meat Carbohydrate Broth Recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium spp. 16x125mm glass tube, needle port hungate cap, 9ml fill, 20/pk AG20H

AnaeroGRO™ Chopped Meat Glucose Broth Recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium spp. 16x125mm glass tube, needle port hungate cap, 9ml fill, 20/pk AG19H AnaeroGRO™ Chopped Meat Broth, AnaeroGRO™ Thioglycollate with Hemin and Cat. no. AG21H Vitamin K (H and K), without Indicator Recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms. 16x125mm glass tube, needle port hungate cap, 7ml fill, 20/pk AG23H 16x125mm glass tube, needle port hungate cap, 9ml fill, 20/pk AG22H

05 ANAEROBE JAR

Holds 14 plates Long-lasting anodized Quad-Ring™ aluminum lid gasket-Best seal in the industry Solid, durable, screw locking lid

Stainless steel rack for Gas-generating easy plate removal sachet

Cat. no. 16000

06 PREPARED CULTURE

MEDIA Bacteroides fragilis on BBE/LKV Biplate, Cat. no. J102.

BE Agar with Azide/CNA Biplate, Cat. no. J66.

Staphylococcus aureus growing on Baird Parker Agar, Cat. no. G96.

Under UV Light Legionella bozemanii (ATCC® 33217) colonies growing on BCYE Selective Agar under UV light, Cat. no. G08.

07 adidophilus (ATCC® 314) growing in Barney Miller Medium, Cat. no. G93.

Anaerobic PEA (Phenylethyl Alcohol) For the isolation of anaerobic bacteria; CDC formula with phenylethanol, 5% sheep blood, hemin, and vitamin K. 15x100mm plate, Barney Miller Medium 10/pk A90 For the determination and identification of beer Baird-Parker Agar spoilage microorganisms. For the selective isolation 15x60mm plate, and enumeration of coagu- 10/pk G93 lase positive staphylococci. Contact plate, BBE Agar 10/pk P16 (Bacteroides Bile Esculin) 15x100mm plate, For the isolation and 10/pk G96 presumptive identification of the Bacteroides fragilis group; for pre-reduced ready-to-use media. (See AnaeroGRO™, page 02) 15x100mm plate, 10/pk G05 BBE Agar/PEA Agar Bacteroide Bile Esculin (BBE) Agar/Anaerobic Phenylethyl Alcohol (PEA) Agar Section I: BBE is recommend- ed for use in the rapid isolation and presumptive identification of Bacteroi- des fragilis group. Section II: PEA is recommended for use as an enriched medium for the cultivation and selective isolation of gram-positive and negative obligate anaerobic bacteria. BE Agar with Azide/CNA 15x100mm biplate, Biplate, Cat. no. J66. 10/pk J122 08 BCYE Selective Agar with CAV BE Agar (Buffered Charcoal Extract Agar with (Bile Esculin) Colistin, Anisomycin, and Vancomycin) For the cultivation and For the selective isolation of differentiation of group D Legionella spp. streptococci and enterococci; 15x100mm plate, inhibits Gram-negative 10/pk G08 bacteria. 15x100mm plate, BCYE Selective Agar with CCVC 10/pk G12 (Buffered Charcoal Yeast Extract Agar with 16x100mm glass tube, Cephalothin, Colistin, Vancomycin, and 5.5ml slant, Cycloheximide) 20/pk L10 For the selective isolation BE Agar with Azide and cultivation of Legionella. 15x100mm plate, (Bile Esculin) For the differentiation of 24ml deep fill, 10/pk G170 group D streptococci and enterococci. BCYE with DGVP 15x100mm plate, (Buffered Charcoal Yeast Extract Agar with 10/pk G11 Dyes, Glycine, Vancomycin, and Polymyxin B) For use in the cultivation BE Agar with Azide/CNA Biplate and primary isolation of (Bile Esculin Agar with Azide/Columbia BBE/LKV Biplate Legionella spp. Agar with 5% Sheep Blood, Colistin, and (Bacteroides Bile Esculin/Laked Blood, 15x100mm plate, Nalidixic Acid) Kanamycin, Vancomycin) 10/pk G209 Section I: BE with Azide Media Section I: BBE is recommend- are recommended for the ed for use in the rapid BCYE Selective Agar with GVPC isolation and differentiation isolation and presumptive (Buffered Charcoal Yeast Extract Agar of group D streptococci identification of Bacteroi- with Glycine, Vancomycin, Polymyxin, from non-group D des fragilis group. and Cycloheximide) streptococci. Section II: Recommended for For the selective isolation of Section II: CNA Agar is use as an enriched medium Legionella spp. recommended for use as a for the cultivation and 15x100mm plate, selective selective isolation of Gram- 26ml deep fill, for the isolation and positive and negative 10/pk W169 differentiation of Gram- obligate anaerobic positive cocci. bacteria. 15x100mm biplate, Under UV 10/pk 15x100mm biplate, Light J66 10/pk J102 BCSA Under UV Light (Burkholderia cepacia Selective Agar) For the selective Legionella isolation and differentiation pneumphila of Burkholderia (Under UV light) (Pseudomonas) cepacia. growing on BCYE with DGVP, 15x100mm plate, 15x100mm plate, 10/pk G09 Cat. no. G209. BCYE Agar (Buffered Charcoal Yeast Extract) For the cultivation of Legionella spp. 15x100mm plate, 10/pk G07 Bacteroides fragilis (ATCC® 25285) colonies growing on Bacteriodes Bile Esculin Agar (Cat. no. G05). Incubated o anaerobically for 24 hours at 35 C. 09 Yeast growing on BiGGY Agar, Cat. no. G17. faecalis (ATCC™ 51299) growing on BHI Agar with Vancomycin, Cat. no. G14. BG Agar (Brilliant Green) For the isolation of Salmonella spp. other than and BHI Agar BHI Agar with Blood Salmonella paratyphi. (Brain Heart Infusion) (Brain Heart Infusion) 15x100mm plate, For the cultivation of For the cultivation of 10/pk G75 microorganisms. microorganisms. BG Agar with Novobiocin Plate 15x100mm plate, 15x100mm, 26ml deep fill, 10/pk A20 (Brilliant Green) 25x100mm plate, 10/pk W15 For the selective isolation 37ml deep fill, and differentiation of 25x100mm, 60ml deep fill, 50/pk W185BX Salmonella spp.; BG 5/pk W163 Tube, 20x125mm glass tube, Agar contains 20μg/ml 10ml slant, Novobiocin. 16x100mm glass, 5.5ml slant, 20/pk L31 20/pk L36 15x100mm plate, HardyFlask™, 12ml slant, 10/pk 20x125mm glass, 10ml slant, G175 20/pk X13 20/pk L35 BG Bile Broth with Durham Tube Flask (Brilliant Green) HardyFlask™, 12ml slant, For the detection of 20/pk X10 coliforms, contains Durham tube for gas detection. 16x125mm glass tube, 10ml fill, 20/pk K66 20x125mm glass tube, 13ml fill, 20/pk K09

10 (ATCC® 25922) growing in Brilliant Green Bile Broth, Cat. no. K09.

Brilliant Green Agar with Novobiocin, Cat. no. G175.

BHI Agar with Blood, 50ml HardyFlask™, slant, 12ml fill, Cat. no. X13.

BHI Agar with Chloramphenicol and Cycloheximide, BHI Agar with Blood and Cat. no. X11. Gentamicin BHI Broth (Brain Heart Infusion) (Brain Heart Infusion) For the selective isolation of For the cultivation of pathogenic fungi. microorganisms. HardyFlask™, 12ml slant, Tubes 20/pk X12 16x125mm glass, 10ml fill, 20/pk K25 BHI Agar, with Blood, 16x100mm Chloramphenicol, and polycarbonate, 5ml fill, Gentamicin 20/pk K27 13x100mm (Brain Heart Infusion) polycarbonate, 2ml fill, For the selective isolation of pathogenic fungi. 20/pk R15 15x100mm plate, BHI Broth with 5% Fildes 26ml deep fill, 10/pk W65 BHI Broth HardyFlask™, 12ml slant, Solution with 5% Fildes 20/pk X14 (Brain Heart Infusion) Solution, Cat. For the cultivation of no. K08 BHI Agar with Chloramphenicol fastidious bacteria and Cycloheximide and capsular strains of (Brain Heart Infusion) . For the selective isolation of 16x125mm glass tube, pathogenic fungi. 10ml fill, HardyFlask™, 12ml slant, 20/pk K08 20/pk X11 BiGGY Agar BHI Agar with Vancomycin (Nickerson Agar, Bismuth Sulfite, Glucose, (Brain Heart Infusion) Glycine, Yeast) An agar screen used for For the selective isolation detection of VRE and identification of Enterococcus spp., with 6μg/ Candida spp. ml Vancomycin. 15x100mm plate, 15x100mm plate, 10/pk G17 10/pk G14 (See HardyCHROM Candida™, page 26) 11 Clostridium perfringens Blood Agar 5%/EMB Biplate (ATCC® 13124) colonies (Tryptic Soy Agar with 5% Sheep Blood/Eosin growing on Brucella Agar with Methylene Blue Agar) H and K, Cat. no. A30. Section I: Blood Agar for use as general purpose growth media for the isolation, cultivation, and differenti- ation of a wide variety of microorganisms. Section II: EMB Agar for- mulations for use as selective and differential media for the isolation of Gram-negative bacilli (including coliform organ- isms and enteric patho- Blood Agar, 5% Sheep blood/ gens) from clinical and EMB Biplate, Cat. no. J22. non-clinical specimens. 15x100mm biplate, 10/pk J22 Blood Agar, 5%/MacConkey Biplate (Tryptic Soy Agar with 5% Sheep Blood/ MacConkey Agar) Section I: Blood Agar products for use as general purpose growth media for the Blood Agar 5%/Blood Agar 5% isolation, cultivation, and Biplate differentiation of a wide (Tryptic Soy Agar with 5% Sheep Blood) variety of microorganisms. Section II: MacConkey Agar For the cultivation of for use as a selective and Blood Agar, 5% microorganisms. differential medium for the Tryptic Soy Agar is a 15x100mm biplate, isolation of Gram-negative nutritious basal medium with 10/pk J93 the addition of 5% sheep bacilli, on the basis of blood provides an excellent Blood Agar, 5%/Chocolate lactose fermentation. 15x100mm biplate, general growth medium. Biplate Contact plate, 10/pk J32 Section I: Blood Agar products 10/pk P33 15x100mm biplate, are recommended for 15x100mm plate, 100/pk J32BX use as general purpose 10/pk A10 growth media for the 15x100mm plate, Blood Agar, 5% with Ampicillin isolation, cultivation, and 100/pk A10BX (Tryptic Soy Agar with 5% Sheep Blood differentiation of a wide 15x100mm plate, and Ampicillin) reduced stacking ring, variety of microorganisms. For the cultivation and Section II: Chocolate Agar 10/pk GA10 selective isolation of is recommended for 15x150mm plate, 70ml fill, Aeromonas spp. use in the isolation and 10/pk H28 15x100mm plate, cultivation of fastidious 16x100mm glass tube, 10/pk A12 5.5ml slant, microorganisms, particularly Haemophilus 20/pk L12 and species. 15x100mm biplate, 10/pk J42

Blood Agar, 5% Sheep blood, Cat. no. P33. 12 Blood Agar, 8% Blood Agar, EH (Tryptic Soy Agar with 8% Sheep Blood) Haemophilus Blood Agar products are influenzae and Streptococcus recommended for use pneumoniae, as general purpose Cat. no. A03. growth media for the isolation, cultivation, and differentiation of a wide variety of microorganisms. 15x100mm plate, 100/pk A17BX Blood Agar, 10% (Tryptic Soy Agar with 10% Sheep Blood) For the cultivation of microorganisms. Blood Agar, EH 15x100mm plate, 10/pk A71 aureus, Cat. no. A03. Blood Agar, EH (Tryptic Soy Agar with 5% Blood, Enhanced Hemolysis Formula) For the cultivation of micro- organisms; Blood Agar, EH produces larger and clearer zones of beta hemolysis. 15x100mm plate, Bovine Blood Agar, 5%, 10/pk A03 with Esculin Blood, Sheep, Defibrinated (Tryptic Soy Agar with 5% Triple Washed Our sheep blood is obtained Bovine Blood and Esculin) from a donor herd specifi- For the detection of organ- cally for Hardy Diagnostics: ism Streptococcus uberis. growing on Blood Agar, sheep are humanely bled 15x100mm plate, Cat. no. A03. and rotated on a veterinary 10/pk A189 supervised program. This ensures blood is kept antibi- Bovine Blood Agar, 5%, with otic-free and as fresh as pos- Esculin/MacConkey Biplate sible, making it less prone to (Tryptic Soy Agar with 5%, Triple Washed Bovine Selective Strep Agar spontaneous hemolysis. Bovine Blood/MacConkey) For the selective isolation of Section I: Bovine Blood Media Glass bottle Streptococcus species from is recommended as a 30ml, Each SB30 bovine mastitis specimens. general purpose growth 50ml, Each SB50 15x100mm plate, media for the cultivation, 10/pk A143 60ml, Each SB60 selective isolation, and 100ml, Each SB100 differentiation of organ- Brucella Agar with 5% Sheep Glass vial, needle port isms responsible for masti- Blood, Hemin, and Vitamin K 100ml, Each SB100S tis in dairy populations. For the cultivation of 500ml, Each SB500S Section II: MacConkey Agar is anaerobic bacteria. Bovine Blood Agar, 5% recommended for use as 15x100mm plate, (Tryptic Soy Agar with 5% Triple Washed a selective and differential 10/pk A30 Bovine Blood) medium for the isolation 15x150mm plate, For the detection of organ- of gram-negative bacilli 10/pk H05 isms that may cause mas- (including coliform titis in dairy cattle such as organisms and enteric Brucella Broth with 15% Glycerol and pathogens), on the basis For the cryopreservation of . of lactose fermentation. organisms by freezing. 15x100mm plate, 15x100mm biplate, 15x45mm glass vial, 2ml fill, 10/pk A188 10/pk J129 100/pk D04

13 Brucella with H and K/LKV Buffered NaCl Peptone EP, USP Campy FDA Agar Biplate For use as a rinse fluid (Brucella Agar with 7% Lysed Horse Blood, (Brucella Agar with 5% Sheep Blood, Hemin for membrane filtration and Polymyxin B, Vancomycin, Amphotericin and Vitamin K /Laked Blood Kanamycin and methods. B, Novobiocin, and Trimethoprim) 500ml polycarbonate bottle, Vancomycin Agar) For the selective isolation of 500ml fill, For the cultivation of Campylobacter spp. 10/pk anaerobic bacteria and U301 15x100mm plate, 10/pk the selective isolation of Butterfield’s Phosphate Buffer A139 Bacteroides spp. For the preparation of Section I: Brucella Agar with H Campy, Blood Free, Karmali Agar dilutions for plate count and and K is recommended for (Karmali Formula, with Charcoal, other laboratory tests. use in the primary isolation Cefoperazone, Vancomycin, and and cultivation of anaero- Dilu-Lok II™, polypropylene vial, Amphotericin B) bic microorganisms. flip-top lid For the selective isolation of Section II: LKV Agar is 145ml, 90ml fill, Campylobacter spp. recommended for use in 50/pk D590 15x100mm plate, the isolation and partial 145ml, 99ml fill, 10/pk G06 identification of anaerobic 50/pk D599 microorganisms. Carrot Broth Polycarbonate bottle/jar 15x100mm biplate, (See Strep B Carrot Broth, page 50) 500ml bottle, 500ml fill, 10/pk J87 10/pk U190 Cetrimide Select Agar, USP For the selective isolation of Buffered Peptone Water Polypropylene bottle . A pre-enrichment medium 125ml, 100ml fill, used to increase the recovery 15x100mm plate, 12/pk U290 of injured Salmonella spp. 10/pk G18 prior to selective enrichment Glass tube and isolation. 16x125mm, 9ml fill, CET/MAC/VJ Triplate Tube 20/pk K109 (Cetrimide Select Agar/MacConkey Agar/ 16x125mm glass, 9ml fill, 20x125mm, 9ml fill, Vogel and Johnson Agar) 20/pk K107 20/pk K209 Section I: CET is for the 16x125mm glass, 10ml fill, Polypropylene tube selective isolation of Pseudomonas aeruginosa. 20/pk K195 13ml, 9ml fill, 20/pk K119 Section II: MacConkey Agar Bottle Campy Cefex Agar, Modified is for the isolation and 500ml polycarbonate, differentiation of Gram- 225ml fill, For the selective isolation of cephalothin resistant negative bacteria. 10/pk U142 Section III: VJ is for the Campylobacter spp. such 500ml polycarbonate, selective isolation of 400ml fill, as , Campylobacter coli, and coagulase-positive 10/pk U143 Campylobacter lari. and mannitol-positive Dilu-Lok™ Vial, 145ml 15x100mm plate, Staphylococcus aureus. polypropylene vial, 15x100mm triplate, 10/pk A122 with flip-top lid 10/pk J314 145ml, irradiated, 90ml fill, Campy CVA Agar 50/pk D080 (Campy Agar with 5% Sheep Blood, Cefo- CGB Agar for Cryptococcus 145ml, irradiated, 99ml fill, perazone, Vancomycin and Amphotericin B) (Canavanine, Glycine, Bromothymol 50/pk D089 For the selective isolation of Blue Agar) Recommended for Buffered Peptone Water with Campylobacter jejuni. 15x100mm plate, the differentiation of 1% Tween® 20 Cryptococcus neoformans 10/pk A40 Pre-filled flip-top dilution var. neoformans and vial format. Cryptococcus gattii 145ml polypropylene vial, (formerly Cryptococcus flip-top lid, 90ml fill, neoformans var. gattii). 50/pk D085 15x100mm plate, 10/pk G113 14 Chocolate Agar (Gonococci Agar Base With 1% Hemoglobin and Enrichments) A nutritious medium used for culturing fastidious organisms such as Haemophilus spp. and Neisseria spp. Plate mirabilis (ATCC® 12453) growth inhibited 15x100mm plate, on Campy Cefex Agar, 10/pk E14 Modified, Cat. no. A122. 15x100mm plate, 100/pk E14BX on Chocolate Agar, Tube Cat. no. E14. 16x100mm glass tube, 5.5ml slant, 20/pk L37 Chocolate/Martin Lewis with Lincomycin Biplate Section I: Chocolate Agar is recommended for use in the isolation and cultivation of fastidious Campylobacter jejuni microorganisms, growing on Campy CVA Agar, particularly Haemophilus Cat. no. A40. and Neisseria species. Section II: Martin Lewis with Lincomycin is a selective medium for the recovery of from both genital and oropharyngeal specimens. 15x100mm biplate, 10/pk J44

Pseudomonas aeruginosa (ATCC® 9027) colonies growing on Cetrimide Selective Agar, Cat. no. G18. Vogel and Johnson

MacConkey

Cetrimide Bacteroides fragilis (ATCC® 25285) colonies growing on Brucella with H and K/LKV Biplate, Cat. no. J87. CET/MAC/VJ Triplate, Cat. no. J314.

15 CompactDry™ is a ready-to-use system reducing the time needed to perform microbial testing on food, beverage, meat, cosmetics, cannabis market, and raw materials. Colonies grow and develop specific colors from chromogenic substrates. This provides for easy organism differentiation and counting.

Room Temp Storage and Long Shelf Life Self-Diffusing Media

Colonies easy to pick and Stackable subculture

Removable Lid

Write-on Area Easy to Pick Up

Features and Benefits: ¨¨Room temperature storage ¨¨18 month shelf life from date of manufacture ¨¨Self diffusing media ¨¨Stackable ¨¨Write-on area ¨¨Easy to handle and pick up ¨¨Simple to pick colonies for subculturing ¨¨Easy read-out of results

CompactDry™ SL CompactDry™ LS (Listeria) (Salmonella) For the selective growth, For the presumptive iden- presumptive identification, tification and enumeration and enumeration of of Salmonella spp. Listeria spp. 16 100/pk SL100 100/pk LS100 CompactDry™ TC CompactDry™ EC CompactDry™ YMR (Total Count) (E. coli and Coliforms) (Yeast and Mold Rapid) CompactDry™ TC for total CompactDry™ EC CompactDry™ YMR, viable bacterial count. contains two kinds of and mold can be Growing colonies appear chromogenic enzyme differentiated by color. red due to redox indicator substrates: Magenta-Gal Molds form colonies with tetrazolium salt. and X-Gluc. E. coli forms a blueish color. 100/pk TPC100 blue colonies, Coliforms 100/pk YMR100 turn red. The total coli- form group count is the sum of both the red and blue colonies. 100/pk EC100

CompactDry™ ETB CompactDry™ XSA () (Staphylococcus aureus) For the isolation and For the selective growth quantification of and differentiation of Enterobacteriaceae Staphylococcus aureus. in foods, cosmetics Staphylococcus aureus or raw materials. CompactDry™YM growth results in blue/ Enterobacteriaceae (Yeast and Mold) blue green colonies. ™ will appear magenta CompactDry YM, yeasts 100/pk XSA100 in color, and are easily and mold can be differ- counted. Genera of entiated by color. Molds Enterobacteriaceae form colonies with a consists of: , blueish color. , Escherichia, 100/pk YM100 , Morganella, Proteus, Salmonella, , , Yersinia and more. 100/pk ETB100 17 DERMATOPHYTE TESTS Derm-Duet™ II DTM/ RSM Biplate (Dermatophytes Test Medium/ Rapid Sporulation Media) Individually wrapped, long shelf life, room temperature storage. 15x100mm biplate, Dermatophyte 10/pk J175 Test Medium, Cat. no. L115. Derm-Duet™ RSM/DTM Biplate (Rapid Sporulation Medium/Dermatophyte Test Medium) For the detection of dermatophytic fungi. 15x100mm biplate, Trichophyton 10/pk J350 mentagrophytes (ATCC® 9533) Dermatophyte Milk Agar growing on For the cultivation and Dermatophyte Test differentiation of certain Medium, dermatophytes especially Cat. no. L115. Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum persicolor. 16x100mm glass tube, 6ml fill, 20/pk L75 Dermatophyte Test Medium Agar For the selective isolation of pathogenic, dermato- phytic fungi. 20x125mm glass tube, 10ml slant, 20/pk L115 Trichophyton 10ml polycarbonate vial, mentagrophytes 7.5ml, slant, (ATCC™ 9533) growing 20/pk L27 on both sections of Derm- HardyFlask™, 12ml slant, Duet™ II, 15x100mm Biplate, 20/pk X15 Cat. no. J175. SabDex Agar with C and HardyFlask™ DTM, G/DTM Biplate Cat. no. X15. (Sabouraud Dextrose Agar with Chlorampheni- col and Gentamicin/Dermatophyte Test Medium) Section I: Sabdex Agar with C and G are recommended for the isolation, cultivation, and maintenance of non-patho- genic and pathogenic spe- cies of fungi and yeasts. Section II: DTM is a selective and differential medium recom- mended for the cultivation and isolation of pathogenic dermatophytic fungi. 15x100mm biplate, 10/pk J107 18 DILUTION VIALS

Dilu-Lok™ eliminates the time and money spent on preparing your own dilution vials! Easy one handed operation.

Dilu-Lok™, polypropylene bottle, flip-top lid, Cat. no. D599.

Dilu-Lok™, Butterfield’s Phosphate Dilu-Lok™, Deionized Sterile Water, Buffer, 145ml polypropylene vial, 145ml polypropylene vial, flip-top lid flip-top lid 25ml fill, 50 pk D125 90ml fill, 50 pk D090 90ml fill, 50 pk D590 99ml fill, 50 pk D099 99ml fill, 50 pk D599 Dilu-Lok™, Peptone Water, 0.1% , Pre-filled Dilution Vials Dilu-Lok™ Buffered Peptone Water, Irradiated, 145ml polypropylene Our pre-filled dilution vials Irradiated, 145ml polypropylene vial, flip-top lid are designed for food, dairy, vial, flip-top lid 90ml fill, 50 pk pharmaceutical and water D290 90ml fill, 50 pk 99ml fill, 50 pk industries. A wide 45mm D080 D299 opening accommodates 99ml fill, 50 pk D089 large samples. A fliptop lid Dilu-Lok™, Phosphate Buffer with allows for easy one-handed Dilu-Lok™, Buffered Peptone MgCl2, 145ml polypropylene vial, opening. Water with 1% Tween® 20, flip-top lid Irradiated, 145ml polypropylene 90ml fill, 50 pk D690 vial, flip-top lid 99ml fill, 50 pk D699 90ml fill, 50 pk D085

19 Bacteroides fragilis (ATCC® 25285) growing in Cooked Meat Medium, Cat. no. K19. CGB Agar for Cryptococcus, Cat. no. G113.

Chocolate/MTM Biplate Chromogenic Media CNA Agar (Chocolate Agar/Modified Thayer Martin) (See HardyCHROM™, page 26) (Columbia Agar with 5% Sheep Blood, Colistin Section I: Chocolate Agar CIN/MacConkey with Sorbitol and Nalidixic Acid) is recommended for For the selective isolation use in the isolation and Biplate and differentiation of Gram- cultivation of fastidious (Cefsulodin Irgasan Novobiocin Agar) positive bacteria. microorganisms, partic- Section I: For the selective 15x100mm plate, ularly Haemophilus and isolation of Yersinia and 10/pk A50 Neisseria species. Aeromonas spp. 15x100mm plate, Section II: Section II: MTM is a selective For the detection of 100/pk A50BX medium used in qual- sorbitol negative enterohe- 15x100mm plate, itative procedures for morrhagic E. coli O157. reduced stacking ring, the isolation of Neisseria 15x100mm biplate, 10/pk GA50 gonorrhoeae with sup- 10/pk J49 pression of most other CIN Agar Gram-negative diplococ- CNA Agar/EMB Agar Biplate ci, Gram-negative bacilli, (Cefsulodin Irgasan Novobiocin Agar) (Columbia Agar with 5% Sheep Blood, Gram-positive organisms, For the selective isolation of Colistin and Nalidixic Acid/Eosin and yeast. and Methylene Blue) 15x100mm biplate, Aeromonas spp. Section I: CNA Agar is 15x100mm plate, 10/pk J72 recommended for use 10/pk G20 as a selective growth Chocolate Agar with Bacitracin medium for the isolation For selective isolation of CLED Agar and differentiation of Haemophilus spp. from respi- (Cystine-Lactose-Electrolyte-Deficient) Gram-positive cocci from ratory specimens. Chocolate For the isolation, enumera- clinical and non-clinical Agar with Bacitracin inhibits tion, and presumptive identi- specimens which contain most staphylococci, strep- fication of urinary pathogens mixed flora. tococci, Nesseria spp., and on the basis of lactose fer- Section II: EMB Agar other normal flora. mentation; controls swarm- formulations are 15x100mm plate, ing of Proteus spp. recommended for use as 10/pk E11 15x100mm plate, selective and differential 10/pk G223 media for the isolation of Gram-negative bacilli from clinical and non-clinical specimens. 15x100mm biplate, 10/pk J52 20 CNA Agar/MacConkey Agar Biplate (Columbia Agar with 5% Sheep Blood, Colistin and Nalidixic Acid/ MacConkey Agar) Section I: CNA Agar is recommended for use as a selective growth medium for the isolation and differentiation of Gram-positive cocci from clinical and non-clinical specimens which contain mixed flora. Section II: A selective and differential medium for the isolation of Gram-negative bacilli, including coliform organisms and enteric pathogens, on the basis of lactose fermen- tation. 15x100mm biplate, 10/pk J62 15x100mm biplate, 100/pk J62BX Columbia Agar, USP For the isolation and cultivation of nonfastidi- ous and fastidious micro-organisms; conforms to harmonized USP/EP/JP requirements. 15x100mm, 10/pk G250 Columbia Agar, 5% Sheep Blood A general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms. 15x100mm, 10/pk A16 15x100mm, 100/pk A16BX Columbia Agar with 5% Sheep Blood/MacConkey Agar Biplate Section I: For the cultivation of microorganisms. Section II: For the isolation and differentiation of Gram-negative bacteria. 15x100mm biplate, 100/pk J57BX Cooked Meat with Iron, H and K For the cultivation of anaerobic bacteria and the preservation of stock cultures; contains iron filings, hemin, yeast extract, and vitamin K. 16x125mm glass tube, 10ml fill, 20/pk K19 Corn Meal Agar with Tween® 80 Recommended for use in cultivation of fungi and for the inducement of chlamydospore formation by Candida spp. Staphylococcus aureus on CNA Agar/ 15x100mm plate, 26ml deep fill, MacConkey Agar Biplate, Cat. no. J62. 10/pk W10 CT-SMAC (Cefixime and Tellurite with MacConkey and Sorbitol) For selection and differentiation of entero- hemorrhagic E. coli O157. 15x100mm plate, 10/pk G129

21 CTA () For fermentation testing of fastidious bacteria. 13x100mm polycarbonate tube, 3ml fill, 20/pk With Cellobiose Y10 With Dextrose Y12 With Lactose Y13 With Maltose Y14 Diamond With Sorbitol Medium Y17 Modified, With Trehalose Y20 Cat. no. K02. D/E Neutralizing Agar (Dey/Engley) For neutralizing and evaluating the efficacy of antiseptics and disinfectants. Dichloran-Glycerol Contact plate, (DG-18) Agar 10/pk P99 For the selective isolation D/E Neutralizing Broth and cultivation of xerophilic molds. (Dey/Engley) 15x100mm plate, For neutralizing and evaluat- 26ml, deep fill, ing the efficacy of antiseptics 10/pk W85 and disinfectants. 16x125mm glass tube, 10ml fill, EC Broth with Durham Tube 20/pk K108 (Escherichia coli Broth) 180ml polycarbonate jar, For the detection of coli- 90ml fill, forms, includes Durham tube D/E Neutralizing 12/pk U75 for gas detection. Broth, 1L polycarbonate bottle, Cat. no. U76. 16x125mm glass tube, 750ml fill, 10ml fill, 10/pk U76 20/pk K63 20x125mm glass tube, DNase Agar with Toluidine Blue 13ml fill, (Deoxyribonuclease) 20/pk K13 For the detection of de- oxyribonuclease activity EC Broth with MUG in Gram-negative bac- and Durham Tube teria, especially Serra- (Escherichia coli Broth with tia spp. and . Methylumbelliferyl Glucuronide) EE Broth Mossel, USP 15x100mm plate, For the detection of E. coli (Enterobacteriaceae 10/pk G24 by fluorescence, includes Enrichment Broth), Durham tube for gas detec- Cat. no. K191. Diamonds Medium, tion; contains methylum- Modified belliferyl glucuronide, read For the selective culti- with a fluorescent lamp, vation of Trichomonas long wave, 366nm, spp., especially Tricho- Cat. no. UVL56. monas vaginalis. 16x125mm glass tube, 16x100mm glass tube, 10ml fill, 6ml fill, 20/pk K64 20/pk K02 20x125mm glass tube, 13ml fill, 20/pk K18

22 Saccharomyces cervisiae growing on Dichloran-Glycerol (DG-18) Agar, Cat. no. W85.

EE Broth Mossel, USP EnteroScreen 4™ (Enterobacteriaceae Enrichment Broth) For screening of isolates of For the selective enrichment enteric pathogens and of Enterobacteriaceae. differentiation of Salmonella EnteroScreen 4™ 16x125mm glass tube, and Shigella. 9ml fill, ¨¨Quickly screen lactose- *Technical 20/pk K191 negative colonies information 180ml po lycarbonate jar, ¨¨Inexpensively detect available upon 90ml fill, Salmonella and request. 12/pk U291 Shigella spp. 180ml polycarbonate jar, ¨¨Inoculate only one tube 100ml fill, per colony rather than 12/pk U391 three or four tubes ¨¨Simply pick a non- LIA with Egg Yolk Agar, Modified lactose fermenting H2S Indicator For the isolation, cultivation, colony and inoculate and differentiation of the Enteroscreen 4™ by Clostridium spp. and other penetrating all four layers anaerobic bacteria. ¨¨Incubate and read results 15x100mm plate, in as early as 6 to 18 hours 10/pk G215 ¨¨Use growth directly LIA without from the slant for PYR, EMB Levine Agar H2S Indicator oxidase, indole, and () antisera confirmation For isolation and 16x125mm glass tube, differentiation of Gram- 4 layer slant, Sterile negative bacilli. 20/pk L225 Petrolatum 15x100mm plate, provides a 10/pk G25 hydrophobic Contact plate, 10/pk P09 barrier

Urea Agar Clostridium sporogenes on Egg Yolk Agar, Modified, Cat. no. G215.

23 EnviroTrans™ Swab Rinse Kit FB Broth Fraser Broth, Modified A ready-to-use swab and (Fastidious Bacteria Broth) For the selective enrichment a pre-filled tube with your For the enriched of Listeria spp., without choice of solutions to fit cultivation of Neisseria, ferric ammonium citrate. your specific needs! Collect Haemophilus, Streptococcus, 16x125mm glass tube, and transport environmental Corynebacteria, and other 10ml fill, 20/pk K98 samples with efficiency fastidious bacteria. and ease. A dacron swab is 16x125mm glass tube, 10ml fill, FTM, USP affixed to a screw cap for 20/pk K31 (Fluid Thioglycollate Broth Medium) easy handling. Recommended for the Polypropylene tube, with dacron Fluid A, USP cultivation of aerobic, swab attached to screw cap: A rinsing and diluting fluid microaerophilic, and for use in USP testing anaerobic microorganisms in With 0.85% Saline, protocols. normally sterile materials. 5ml fill, Glass bottle, needle port USP, boston round glass bottle 20/pk SRK35 With 0.1% peptone, 100ml, 250ml, 200ml fill, 12/pk With D/E Neutralizing Broth crimp cap, 100ml fill, U434 5ml, 20/pk U109 USP, glass vial, needle port Polypropylene bottle 20/pk SRK45 20ml, crimp cap, 15ml fill, 125ml, 100ml fill, 50/pk U66 With Letheen Broth 12/pk U19 20ml, crimp cap, 20ml fill, 5ml, 1L, 1000ml fill, 50/pk U68 20/pk SRK25 10/pk U208 USP, glass bottle, needle port With Neutralizing Buffer 100ml, crimp cap, 5ml, Fluid D, USP A rinsing and diluting 100ml fill, 20/pk SRK15 fluid for use in USP 20/pk U84 testing protocols. 200ml, crimp cap, 150ml fill, Polycarbonate bottle 10/pk 500ml, needle port, U207 500ml, 300ml fill, 300ml fill, 10/pk U210 10/pk U427 500ml, needle port, 500ml, 500ml fill, 500ml fill, 10/pk U273 10/pk U215 Jar 1L, bottle, 1000ml fill, 236ml glass, 100ml fill, 10/pk U115 12/pk U41 Glass bottle Glass tube, 100ml, needle port, crimp needle port hungate cap cap, 100ml fill, 16x125mm, 10ml fill, 20/pk U110 20/pk K282 Fluid K, USP Glass tube A rinsing and diluting fluid 16x125mm, 10ml fill, for use in USP 20/pk K21 testing protocols. 1L polycarbonate bottle, 1000ml fill, 10/pk U206

EnviroTrans™ Swab Rinse Kit

24 Fluid A, USP, 100ml bottle with needle port, crimp cap, 100ml fill, Fluid Cat. no. U109. Thioglycollate Broth Medium (FTM) with Indicator, USP, glass tube, 16x125mm, 10ml Haemophilus ID Quadplate, fill, Cat. no. K21. Cat. no. J82.

FTM with Lecithin and Tween® Haemophilus ID Quadplate (Fluid Thioglycollate Medium) GN Broth For the differentiation of For use in the cultivation of Haemophilus spp. aerobic microaerophilic, and (Gram-Negative) Section I: RTF Casman anaerobic mircroorganisms For the selective Medium, Modified in normally sterile materials. enrichment of Salmonella (X- and V-factors) is 200ml glass vial, needle and Shigella spp. supplemented with sheep port, crimp cap, 150ml fill, Polycarbonate tube, 6ml, blood in order to supply 20/pk 10/pk U430 K01 hemin (X-factor), and 16x125mm glass tube, 10ml nicotinamide adenine FTM with Hemin and Vitamin K fill, dinucleotide (NAD or Fluid Thioglycollate Medium with Hemin 20/pk K39 V-factor) which are and Vitamin K 12x80mm polypropylene growth factors required by tube, 4ml, For the cultivation of aer- Haemophilus influenzae. 100/pk obic, microaerophilic, and R76BX Section II: Tryptic Soy anaerobic microorganisms in Granada Medium Agar (TSA) with Hemin normally sterile materials. (See Strep B Carrot Broth™ page 50) (X-factor) 16x125mm glass tube, Section III: Tryptic Soy Agar 10ml fill, 20/pk K73 Group A Beta Strep Agar (TSA), modified with NAD For the selective isolation (V-factor) GBS Detect™ of Group A Strep only Section IV: Chocolate Agar For the isolation and differ- (Streptococcus pyogenes); (X- and V-factors) entiation by enhanced he- inhibits other Streps and 15x100mm quadplate, molysis of gamma hemolytic normal flora. Contains 5% 5ml/quadrant, Group B Streptococcus. sheep blood, , 10/pk J82 (See Strep B Carrot Broth™ page 50) colistin, and SXT. 15x100mm plate, 15x100mm plate, 10/pk 10/pk A300 A72 15x100mm plate, (For selection of all streps, see Selective Strep 100/pk A300BX Agar, page 48) 25 Hardy TM HardyCHROM™ BluEcoli™

HardyCHROM™ BluEcoli™ Biplate Used for the culture and isolation of urinary pathogens and for the confirmatory identification of E. coli. Colonies turn a distinct blue color within 24 hours on the chromogenic side. Found to be 98.5% sensitive and 100% specific for urine specimens. No further testing required: 75 to 90% of positive urine specimens are E. coli. BluEcoli™/Blood Agar, 15x100mm biplate, 10/pk J123

HardyCHROM™ Candida HardyCHROM™ Candida HardyCHROM™ Candida is a selective and differential culture medium that facilitates the isolation and differentiation of clinically important yeast species. ¨¨Useful in detecting mixed yeast infections ¨¨Inhibits the majority of bacterial species 15x100mm plate, 10/pk G301

HardyCHROM™ ECC (E. coli and Coliforms) A differential chromogenic medium used for rapid and reliable detection and differentiation of E. coli from other coliforms. E. coli can be identified as pink colonies HardyCHROM™ ECC on the plate, while other coliform bacteria will appear as turquoise colonies. 15x100mm plate, 10/pk G303 (Coliform)

Escherichia coli

26 HardyCHROM™ ESBL (Extended-Spectrum Beta-Lactamase) A selective chromogenic medium recommended for primary screening and Klebsiella spp. differentiation of ESBL in Proteus spp. Enterobacteriaceae. ¨¨Results in as little as 18 hours ¨¨Easy-to-read color read- out 15x100mm plate, ESBL producing 10/pk G321 Klebsiella pneumoniae, Cat. no. G321. HardyCHROM™ HUrBi™ (Urine Biplate) HardyCHROM™ HUrBi™ is HardyCHROM™ ESBL formulated to isolate Gram- positive organisms and yeast on the left side of the biplate and Gram-negative organisms on the right side HardyCHROM™ HUrBi™ of the biplate. 15x100mm biplate, Gram- Gram- 10/pk J100 Positive Negative HardyCHROM™ Listeria A chromogenic medium recommended for the isolation, differentiation, Escherichia coli Staphylococcus and enumeration of Listeria (Dark Pink) aureus (White) monocytogenes from food Enterococcus Klebsiella and environmental faecalis (Blue) pneumoniae samples by colony color (Dark Blue) Staphylococcus and appearance. saprophyticus 15x100mm plate, (Magenta) 10/pk G317 HardyCHROM™ MRSA A selective and differential chromogenic medium recommended for the Pseudomonas qualitative detection aeruginosa of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA). Not intended to diagnose MRSA infection or to guide or monitor therapy HardyCHROM™ MRSA for MRSA infection. ¨¨Distinct color change read-out ¨¨Bright color development ¨¨Economically priced ¨¨Read-out at 24 hours Methicillin-resistant Staphylococcus aureus colonies 15x100mm plate, grown aerobicallyfor 24 hours, Cat. no. G307. 10/pk G307

27 HardyCHROM™ O157 HardyCHROM™ SS NoPRO A selective and differential In 1968, Hektoen Enteric medium recommended Agar was introduced to se- for the isolation of ente- lect for most Salmonella and H2S producing Salmonella Showing colonies with ro-hemorrhagic E. coli O157. Shigella (high sensitivity). large black centers with Chromogenic substances Unfortunately it also pro- a clear perimeter. in the medium facilitate de- duces many annoying false tection by colony color. Not positives (low specificity). Escherichia coli Showing small pink for human diagnostic use. This chromogenic medium colonies. 15x100mm plate, for Salmonella and Shigella 10/pk G305 is both sensitive and specific without needless work-ups Showing teal-colored HardyCHROM™ Sakazakii colonies. A selective and differen- for Proteus. tial chromogenic medium 15x100mm plate, Proteus spp. You won’t find it here! recommended for the 10/pk G327 isolation and detection of Cronobacter (Enterobacter) sakazakii. 15x100mm plate, 10/pk G315 HardyCHROM™ Salmonella A selective and differential medium for the isolation and HardyCHROM™ SS NoPRO differentiation of Salmonella Cat. no. G327. spp. from other members

of the Enterobacteriaceae based on colony color. All A60 no. Cat. Agar, LKV Salmonella spp. including Contains a Salmonella typhi and Salmo- proprietary nella paratyphi A, produce antibiotic that deep pink colonies. Other eliminates the occurrence of members of the Entero- Proteus! bacteriaceae produce blue, white, or colorless colonies, while Gram-positive bacteria Plate incubated aerobically for 24 hours at 35 ºC. will be inhibited. 15x100mm plate, 10/pk G309 HardyCHROM™ Salmonella/ XLT-4 Agar Biplate, Manufactured and sold in the US 15x100mm, under license from Glycosynth 10/pk J37 Limited under U.S. Patent No.’s 7,323,488 B2, 7,384,763 B2, and 7,709,223 B2.

HardyCHROM™ O157

Salmonella enterica growing on HardyCHROM™ Salmonella, Escherichia coli O157 growing on Chronobacter sakazakii growing on 28 Cat. no. J37. HardyCHROM™ O157, Cat. no. G305. HardyCHROM™ Sakazakii, Cat. no. G315. HardyCHROM™ Staph aureus For the rapid and reliable Staphylococcus aureus growing on detection of Staphylococ- HardyCHROM™ Staph aureus (top), cus aureus within 24 hours. Staphylococcus saprophyticus (bottom), Staphylococcus aureus Cat. no. G311. will produce deep pink to fuschia colonies. Selective agents inhibit the growth of Gram-negative organisms, yeast, and some Gram-posi- tive cocci. 15x100mm plate, 10/pk G311

HardyCHROM™ UTI primary set-up of urine A culture medium that specimens. HardyCHROM™ facilitates the isolation and UTI can be used to identify differentiation of common E. coli and Enterococcus spp. urinary tract pathogens, based on their characteristic including Gram-negative color reactions, with no and Gram-positive further testing needed. bacteria. The development 15x100mm plate, of various colors, due to 10/pk G313 chromogenic substances in the medium, allows for the differentiation of microorganisms from the Staphylococcus saprophyticus

Citrobacter spp. Staphylococcus aureus Escherichia coli Proteus mirabilis

Klebsiella pneumoniae Candida albicans Pseudomonas aeruginosa 29 Hardy TM Chromogenic Culture Media

HardyCHROM™ Vibrio HardyCHROM™ Vibrio is a selective and differential screening media recommended for the isolation of Vibrio Vibrio pathogenic Vibrio spp. from food vulnificus cholerae and environmental sources. HardyCHROM™ Vibrio was developed as a medium for Under UV Light differentiating , and from other Vibrios based on colony color and fluorescence under UV light on a single plate. 15x100mm plate, 10/pk G319 Vibrio algonyticus *Also available in Dehydrated Culture Media (Cat. nos. C9010, C9011, C9012, C9013)

Vibrio parahaemolyticus

Vibrio cholerae

Vibrio vulnificus

HE Agar (Hektoen Enteric) For the selective isolation of HTM Agar for Haemophilus pathogenic, Gram-negative, spp., Cat. no. G33. enteric bacteria, such as Salmonella spp. and Shigella spp. 15x100mm plate, 10/pk G63

Salmonella enterica (ATCC® 14028) colonies growing on Hektoen 30 Enteric Agar, Cat. no. G63. Salmonella enterica growing on SS agar, HE Agar/SS Agar Biplate, Cat. no. J139.

HE Agar/SS Agar Biplate Inhibitory Mold Agar with (Hektoen Enteric/Salmonella Shigella) Gentamicin, Cat. no. W27. For the selective and differential isolation of pathogenic enteric bacteria. Section I: HE Agar is a selective and differential Inhibitory Mold Agar medium used for the For the selective isolation of KF Streptococcus Agar isolation and differentiation pathogenic fungi, contains For the selective isolation of Gram-negative enteric chloramphenicol for and enumeration of fecal pathogen. inhibition of bacteria. streptococci (including Section II: SS Agar is 15x100mm plate, Enterococcus). recommended for use 26ml deep fill, 15x100mm plate, as a selective and 10/pk W25 10/pk G376 differential medium for 20x125mm glass tube, KIA Slant the isolation of Salmonella 10ml slant, (Kligler Iron Agar) and some Shigella species 20/pk L47 For the identification of from clinical and non- HardyFlask™, 12ml slant, Gram-negative enteric clinical specimens. 20/pk X20 15x100mm biplate, bacteria based on hydrogen sulfide production and the 10/pk J139 Inhibitory Mold Agar with Gentamicin fermentation of dextrose and lactose. HTM Agar For the selective isolation 13x100mm glass tube, (Haemophilus Test Medium) of pathogenic fungi; 4.5ml fill, 20/pk For disk diffusion contains chloramphenicol R70 16x125mm glass tube, susceptibility testing of and gentamicin. Haemophilus spp. 15x100mm plate, 8ml fill, 20/pk L70 15x100mm plate, 26ml deep fill, Lactobacilli MRS Agar 10/pk G33 10/pk W27 For the isolation, 15x150mm plate, 20x125mm glass tube, enumeration, and cultivation 10/pk H07 10ml slant, of Lactobacillus spp. 20/pk L49 15x60mm plate, Iodine-Iodide Solution 10/pk G197 For use with Tetrathionate 15x100mm plate, Broth, Cat. no. K65. 10/pk G117 1L polycarbonate bottle, 990ml fill, Each Z139 31 Lactobacilli MRS Broth Leeds Medium For the cultivation of For the selective isolation lactobacilli. and differentiation of Glass tube Acinetobacter spp. Lactobacillus 16x125mm, 9ml fill, 15x100mm plate, fermentum 10/pk G261 (ATCC® 9338) 20/pk K15 growing in 20x125mm, Letheen Agar Lactobacilli MRS 20/pk K317 For enumeration of Broth, 16x125mm, with Durham microorganisms and Cat. no. K17. tube, 9ml fill, evaluation of disinfectants in 20/pk K17 cosmetic products. Bottle 15x100mm plate, 500ml polycarbonate bottle, 10/pk G42 500ml fill, 20x125mm glass pour Durham tube with 10/pk U203 tube, 18ml deep fill, trapped gas bubble. 20/pk Q18 Lactose Broth For the cultivation of coli- Letheen Agar, Modified forms and Salmonella spp. For enumeration of microorganisms and Polypropylene bottle evaluation of disinfectants in 500ml bottle, 225ml fill, cosmetic products. 10/pk U145 15x100mm plate, 10/pk G221 Lauryl Tryptose Broth with Durham Tube Letheen Agar, Modified with For the detection of Tween® 80, 1.5% LIA (Lysine coliforms. For enumeration of Iron Agar) Glass tube microorganisms and Slant, Cat. no. R22. 16x125mm, Single Strength, evaluation of disinfectants 10ml fill, in cosmetic products. 20/pk K61 15x100mm plate, 20x125mm, Single Strength, 10/pk W55 13ml fill, 20/pk K33 20x125mm, Double Strength, 10ml fill, 20/pk K32

LIA () Slant, Cat. no. L25.

Acinetobacter baumannii (ATCC® BAA-1605) colonies growing on Leeds Medium, Cat. no. G261. 32 Letheen Agar/MacConkey Agar LIA LJ Medium Biplate (Lysine Iron Agar) (Lowenstein Jensen) Section I: Letheen Agar is For the differentiation of For the cultivation of used for enumeration of Gram-negative enteric Mycobacterium spp. microorganisms and eval- pathogens from normal 20x125mm glass tube, uation of disinfectants in enteric flora by hydrogen 10ml slant, cosmetic products. sulfide production, lysine 20/pk C21 Section II: MacConkey Agar is deaminase, and lysine HardyFlask™, 12ml slant, used for the isolation and decarboxylase reactions. 20/pk X22 differentiation of Gram- 13x100mm tube, 4.5ml slant, negative organisms. 20/pk R22 LJ with Ferric Ammonium 15x100mm biplate, 16x125mm tube, 8ml slant, Citrate, Slant 20/pk L25 10/pk J110 (Lowenstein Jensen) Liver Infusion Agar Selective Letheen Broth For the cultivation of (Liver Infusion Selective Agar with Polymyxin Mycobacterium spp. For cultivation of B, Nalidixic Acid, Vancomycin) HardyFlask™, microorganisms and For the selective isolation of 20/pk X21 evaluation of disinfectants in fungi. cosmetic products. 15x100mm plate, LJ with Pyruvate Glass tube 10/pk W95 (Lowenstein Jensen) 16x125mm, 9ml fill, For the cultivation of Myco- 20/pk K207 LJ Gruft bacterium spp., especially 16x125mm, 10ml fill, (Lowenstein Jensen) Mycobacterium bovis. 20/pk K106 For the selective isolation HardyFlask™, 12ml slant, of Mycobacterium spp.; Polypropylene bottle 20/pk X19 1L, 1000ml fill, contains penicillin, nalidixic acid, and RNA. 10/pk U371 Jar 20x125mm glass tube, 10ml fill slant, 236ml wide mouth glass jar, 20/pk C23 90ml fill, 12/pk U39 HardyFlask™, 12ml slant, Letheen Broth, Modified 20/pk X23 For cultivation of microorganisms and evaluation of disinfectants in cosmetic products. 236ml wide mouth glass jar, 90ml fill, 12/pk U293

Lactobacillus acidophilus (ATCC® 4356) colonies growing on Lactobacilli MRS Agar, Cat. no. G117.

Letheen Agar/MacConkey Agar Biplate, Cat. no. J110. 33 LKV Agar (Brucella Agar with Laked Sheep Blood, Kanamycin, Vancomycin, Hemin, and Vitamin K) For the selective isolation of Gram-negative anaerobic bacteria, especially Bacteroides spp. 15x100mm plate, 10/pk A60

Loeffler’s Slant For the cultivation of Corynebacterium diphtheriae. 16x100mm glass tube, 5.5ml slant, 20/pk L28

m EI Agar (Enterococci Indicator) For the selective chromogenic detection and enumeration of enterococci. 15x60mm plate, 10/pk G124 m Endo LES Agar Escherichia coli growing on For the enumeration of coliforms. MacConkey Agar, USP, 15x60mm plate, Cat. no. G35. 10/pk G128 15x100mm plate, 10/pk G28

m FC Agar with 1% Rosolic Acid (Membrane- Medium) For the isolation and enumeration of fecal coliforms based on lactose fermentation. 11x50mm plate, 10/pk G272 15x60mm plate, 10/pk G126

HardyFlask™ LJ with Iron, Cat. Pseudomonas aeruginosa no. X21. growing on m-PA Agar, Cat. no. G133.

34 m HPC Agar MacConkey Agar with (Heterotrophic Plate Count) Cefotaxime For the enumeration of For the selective cultivation heterotrophic organisms. of Gram-negative bacteria. 15x100mm plate, 15x100mm plate, 10/pk G195 10/pk G121 m PA Agar MacConkey Agar with Sorbitol (Pseudomonas aeruginosa) For the detection of E. coli For the cultivation O157 (sorbitol negative). and enumeration of 15x100mm plate, Pseudomonas aeruginosa. 10/pk G36 15x60mm plate, 10/pk G133 MacConkey/EMB Biplate (Eosin Methylene Blue) m TEC Agar, Modified Section I: MacConkey Agar is For selective chromogenic recommended for use as differentiation and enumera- a selective and differential tion of Escherichia coli. medium for the isolation 15x60mm plate, of Gram-negative bacilli 10/pk G106 (including coliform organisms and enteric MacConkey Agar pathogens), on the basis of For isolation and lactose fermentation. differentiation of Gram- Section II: EMB Agar negative bacteria. formulations are Plate recommended for use as USP, 15x100mm, selective and differential m FC Medium 10/pk media for the isolation G35 with 1% Rosolic Acid, USP, 15x100mm, of Gram-negative bacilli Cat. no. G126. 100/pk G35BX (including coliform 15x100mm, organisms and enteric reduced stacking ring, pathogens) from clinical Fecal 10/pk GA35 and nonclinical specimens. Coliforms 15x100mm biplate, 10/pk J58

Membrane filtration technique. Bacteroides fragilis (ATCC ® 25285) colonies growing on m El Agar, Cat. no. G124 LKV Agar (Cat. no. A60).

35 Pill pocket for CO2 generating tablet.

Bacteroides fragilis (ATCC ® 25285) colonies growing on Neisseria gonorhoeae Neomycin Anaerobic growing on Martin Blood Agar Lewis with Lincomycin, (Cat. no. A62). 15x100mm pill pocket

plate with bag and CO2 tablet, Cat. no. E31.

Mastitis Triplate (Blood Agar/ Salmonella enterica growing on MacConkey Agar/ MacConkey/EMB (Eosin Methylene Blue) TKT Agar), Biplate, Cat. no. J58. 15x100mm, Cat. no. J312.

Middlebrook 7H10 Agar, Cat. no. W30.

MacConkey Broth Malt Extract Agar with Lecithin Martin Lewis with Lincomycin For the detection of Gram- and Tween (Martin Lewis Agar with Lincomycin, negative, lactose Contact plate, Colistin, Amphotericin, Trimethoprim, fermenting bacilli. 10/pk P93 and Vancomycin) 15X103mm polycarbonate Malt Extract Agar with 0.01% For the selective isolation tube, with Durham tube, of Neisseria gonorrhoeae. 5ml fill, Chloramphenicol Pill pocket plate with bag 20/pk K194 For the selective isolation and CO2 tablet, 180ml wide mouth of fungi. 10/pk E31 polycarbonate jar, 100ml fill, 15x100mm plate, 15x100mm plate, 12/pk U125 26ml deep fill, 10/pk E39 Malt Extract Agar 10/pk W80 For the cultivation and enumeration of yeasts and molds. 15x100mm plate, 26ml deep fill, 10/pk W28

36 Mastitis Triplate (TKT Agar/MacConkey Agar/Blood Agar) For the isolation of MacConkey Broth, organisms responsible Cat. no. U125. Middlebrook 7H9 for mastitis in dairy Broth, populations. 16x125mm tube, 10ml fill, Section I: TKT Agar is used Cat. no. C32. in the selective isolation and differentiation of Streptococcus species. Section II: MacConkey Agar is used for the selective isolation and differentiation of coliforms. Section III: Tryptic Soy Agar (TSA) with blood agar, is used to grow all organisms as well as demonstrate hemolysis patterns. 15x100mm triplate, 7ml fill, 10/pk J312 Group IV (ATCC® 6841) McFarland Standard colonies growing on McFarland Latex Standards Middlebrook 7H11 Agar Middlebrook 7H11 Agar, are more stable and offer a For the cultivation of Cat. no. SP57. significantly longer shelf- Mycobacterium spp. life than the original barium 15x100mm plate, sulfate standards. 28ml deep fill, 10/pk W35 Middlebrook 7H9 Broth 16x100mm glass tube, 8ml fill 20x125mm glass tube, 10ml slant, For the cultivation of # 0.5, Each ML05 20/pk Mycobacterium spp. # 1, Each ML1 C36 HardyFlask™, 12ml slant, 16x125mm glass tube, # 2 Each ML2 10ml fill, 20/pk X25 # 3, Each ML3 20/pk C32 # 4, Each ML4 # 5, Each ML5 Middlebrook 7H11 Selective Middlebrook 7H9 Broth with # 6, Each ML6 For selective isolation Tween® 80 # 7, Each ML7 of Mycobacterium spp.; For the cultivation of # 8, Each ML8 contains carbenicillin, Mycobacterium spp. amphotericin B, polymyxin Middlebrook 7H10 Agar 16x125mm glass tube, 5ml fill, B, and trimethoprim lactate. 20/pk C62 For the cultivation of 20x125mm glass tube, Mycobacterium spp. 10ml slant, MIL Medium 15x100mm plate, 20/pk C38 (Motility, Indole, Lysine Medium) 26ml deep fill, 15x100mm plate, For the determination 10/pk W30 28ml deep fill, of motility, indole, lysine 20x125mm glass tube, 10/pk W40 deamination, and lysine 10ml slant, HardyFlask™, 12ml slant, decarboxylation of Gram- 20/pk C34 20/pk X28 negative bacilli. HardyFlask™, 12ml slant, 16x100mm glass tube, 20/pk X26 Middlebrook 7H11, Thin Pour 8ml deep fill, For the isolation and 20/pk Q09 Middlebrook 7H11/7H11 Selective detection of mycobacteria Agar Biplate using the micro-colony For the cultivation of method. Thin pour plate for Mycobacterium spp. microscopic examination. 15x100mm biplate, Use with MycoSeal™, 10/pk J75 Cat. no. SS9225. SpaceSaver™ plate, 10x100mm, thin pour, 37 15/pk SP57 Motility Test Medium without TTC For the detection of motility in bacteria, without triphenyltetrazolium chloride (TTC) growth indicator. 16x100mm glass tube, Motility Test Medium with 8ml deep fill, TTC 20/pk Q10 Escherichia MRSA Screen Plate coli (ATCC® Motile (Methicillin-resistant Staphylococcus aureus 25922), Bacteria with 4% NaCl and Oxacillin 6μg/ml) Cat. no. Q11. Screen isolates to determine methicillin-resistance in Staphylococcus aureus. 15x100mm plate, 10/pk G47 (See HardyCHROM™ MRSA, page 27) MR-VP Broth (Methyl Red and Voges-Proskauer) MIO Medium For the identification of (Motility, Indole, and Ornithine Gram-negative bacilli. Decarboxylase) 15x103mm polycarbonate tube, 5ml fill, For the determination of 20/pk motility, indole, and ornithine K37 decarboxylation of Gram- MSA Agar negative bacilli. (Mannitol Salt) 13x100mm glass tube, 4ml fill, For the selective isolation 20/pk R24 and differentiation of 16x100mm glass tube, Staphylococcus spp. 6.5ml fill, 15x100mm plate, 20/pk Q20 10/pk G40 Moeller’s Decarboxylase MSA with Oxacillin For the differentiation of ( with 6.5% NaCl and Gram-negative enteric 4μg/ml Oxacillin) bacilli based on the Screen isolates to determine decarboxylation reaction of methicillin-resistance in MIL Medium, amino acids. Staphylococcus aureus. 16x125mm glass tube, 5ml fill, Cat. no. Q09. 15x100mm plate, 20/pk 10/pk G97 With Arginine Y42 With Lysine Y43 Mueller Hinton Agar With Ornithine Y44 For antimicrobial susceptibil- Base Medium, Control Y41 ity testing of non-fastidious bacteria using the disk diffu- Motility Test Medium with TTC sion (Kirby-Bauer) method. For the determination 15x100mm plate, 28ml fill, of motility in Gram- 10/pk G45 negative bacteria, contains 15x150mm plate, 69ml fill, triphenyltetrazolium chloride 10/pk H11 (TTC) growth indicator. 16x100mm glass tube, 8ml deep fill, 20/pk Q11

38 Left Positive: grown in MR-VP Broth. Right Negative: Enterobacter cloacae grown in MR-VP Broth.

Mueller Hinton Agar with Blood (Mueller Hinton Agar with 5% Sheep Blood) For disk diffusion (Kirby- Bauer) susceptibility testing of Streptococcus spp. including . 15x100mm plate, 25ml fill, 10/pk A59 15x150mm plate, 72ml fill, 10/pk H21 Mueller Hinton Broth For the preparation of suspensions for disk diffusion (Kirby-Bauer) Mueller Hinton susceptibility testing. Agar, Cat. 13x100mm glass tube, 3ml fill, no. H11 20/pk R38 Mycobiotic Agar For the selective isolation of pathogenic fungi, especially from hair, skin, and nail specimens; contains chloramphenicol and cycloheximide. 15x100mm plate, 26ml deep fill, 10/pk W50 20x125mm glass tube, 10ml slant, 20/pk L45 HardyFlask™, 12ml slant, 20/pk X30 Mycoplasma Agar For the detection of Myco- plasma spp. associated with bovine mastitis. 15x100mm plate, 10/pk G102 Mycoplasma Broth For the detection of Myco- plasma spp. associated with bovine mastitis. 3913x100mm polycarbonate tube, 4ml fill, Mycoplasma bovis growing on 20/pk R102 Mycoplasma Agar, Cat. no. G102

39 MYP Agar NaCl (Salt Tolerance) 6.5% Broth Neomycin Anaerobic Blood Agar (Mannitol Yolk Polymyxin Agar) For the identification of (Blood Agar with 5% Sheep Blood, Hemin, For the enumeration of Enterococcus spp. Vitamin K, and Neomycin) cereus from foods. Tube with Indicator For the selective isolation of 15x100mm plate, 13x100mm polycarbonate, anaerobic bacteria. 10/pk G147 1ml fill, 15x100mm plate, 20/pk R26 10/pk A62 15x103mm polycarbonate, 2ml fill, Neutralizing Buffer 20/pk K49 Neutralizes the bacteriostat- ic effects of chlorine and quaternary ammonia com- pounds, allowing recovery of sensitive organisms. 16x125mm glass tube, 10ml fill, 20/pk K105 Neomycin Anaerobic Blood Agar, 15x100mm plate, Cat. no. A62.

Acanthamoeba castellani (ATCC® 30010) growing in Non-nutrient Agar plate, Cat. no. G225.

Nutrient Agar with MUG, Cat. no. G114.

MycoVue™ Slide Culture System MycoVue™ is a ready-to-use slide cul- ture system for the cultivation of fungi. Simplify your fungal slide cultures while saving money, reducing frustration, and eliminating preparation time. Desicca- tion is prevented with a built-in humidity chamber and the culture can be incubat- ed for long periods of time. Device fits ™ conveniently on the microscope stage MycoVue for easy observation without disturbing fungal structures. Each unit contains: ¨¨1 agar block in a molded plastic con- tainer, covered by a plastic tab ¨¨1 humidifying chamber The ultimate ready-to-use method for slide Fungal Slide ¨¨1 plastic coverslip culturing. View directly on the microscope. Culture Made Easy! ¨¨1 plastic lid Potato Flake Agar, 40 5 trays/pk MV1 growing on PALCAM, Cat. no. G149.

Nitrate Broth with Durham Tube Escherichia coli (ATCC ® 25922) For the determination of growing in ONPG Rapid Test Broth nitrate reduction and Cat. no. R92. gas production. OF Media 15x103mm polycarbonate tube, For the detection of 20/pk K42 carbohydrate oxidation or Non-Nutrient Agar Plate fermentation by bacteria, OF Media, with Dextrose (positive), For the detection of free- base medium does not Cat. no. Y57. living amoebae, including contain carbohydrate. Acanthamoeba and 13x100mm polycarbonate tube, Page’s Saline Naegleria spp. 3ml fill, 20/pk For detecting the presence 15x100mm plate, 20ml fill, With Dextrose Y57 of free-living amoebae cysts 10/pk G225 and trophozoites in tissue, ONPG Rapid Test Broth soil, or water samples; for use Nutrient Agar For the rapid determination with Non-Nutrient Agar, For the cultivation of non- of beta-galactosidase activi- Cat. no. G225. fastidious microorganisms. ty in Gram-negative bacilli. 13x100mm polycarbonate 15x100mm plate, 13x100mm polycarbonate tube, 2ml fill, 20/pk R225 26ml deep fill, tube, 0.5ml fill, PALCAM 10/pk W51 20/pk R92 (Polymyxin Acriflavin Lithium-chloride Glass tube Orange Serum Agar 16x100mm, 5.5ml slant, Ceftazidime Esculin Mannitol) For the cultivation and 20/pk L20 For the isolation and enumeration of aciduric cultivation of Listeria spp. Nutrient Agar with MUG bacteria and fungi. 15x100mm plate, (Methylumbelliferyl Glucuronide) 15x60mm plate, 11ml fill, 10/pk G149 For the detection and 10/pk G91 enumeration of E. coli. 15x100mm plate, 18ml fill, PC Agar 15x60mm plate, 10/pk G181 (Pseudomonas cepacia) 10/pk G114 For the selective isolation Oxford Medium, Modified of Pseudomonas Nutrient Broth For the selective isolation (Burkholderia) cepacia. A general purpose growth and differentiation of 15x100mm plate, Listeria monocytogenes. medium recommended 10/pk G48 for use in the cultivation 15x100mm plate, of nonfastidious 10/pk G46 microorganisms. 15x103mm polycarbonate tube, 5ml fill, 20/pk K43 125ml polypropylene bottle, 100ml fill, 12/pk U234 41 Burkholderia (Pseudomonas) Trichophyton rubrum cepacia (ATCC® 25416) colonies (ATCC® 28188) growing on growing on PC Agar, Potato Dextrose Agar, Cat. no. G48. Cat. no. W60.

Phenol Red Broth with Durham Tube For the differentiation of microorganisms by Staphylococcus aureus carbohydrate fermentation. colonies growing on PEA with 5% Phenol Red Broth Sheep Blood, Cat. no. A93. contains a Durham tube Phenylalanine Agar Slant for gas detection; base medium does not contain (Phenylalanine alcohol) carbohydrate. For the differentiation 16x125mm glass, 10ml fill, 20/pk of Gram-negative bacilli With Dextrose based on the production Y304 Peptone Water With Lactose Y309 of phenylpyruvic acid by (Peptone Broth, Peptone Salt) oxidative deamination. 10%, For use as a diluent and Phosphate Buffered Saline Cat no. Z63 not included. for the preparation of 16x100mm glass tube, (PBS), pH 6.8 suspensions, contains 0.1% For preparation of specimens 6.5ml slant, Proteose Peptone. 20/pk L21 for AFB culture. With Sodium Chloride: Tube PEA with 5% Sheep Blood with 1% Sodium Chloride, 13x100mm polycarbonate, (Phenylethyl Alcohol Agar) 20x125mm glass tube, 3ml fill, For use in the cultivation 9ml fill, 20/pk R196 and selective isolation of 20/pk K47 anaerobic Gram-positive Without Sodium Chloride: Bottle bacteria. 500ml polycarbonate bottle, 60ml polypropylene, 15x100mm plate, 500ml fill, 40ml fill, 10/pk A93 10/pk U201 25/pk X43 500ml polycarbonate, Peptic Digest Agar with Fildes Peptone Salt 500ml fill, and Nafcillin For use as a diluent and Each U10 For the selective isolation for the preparation of Phosphate Buffered Saline suspensions. Contains 0.1% and cultivation of (0.01M) with 0.02% Tween® 80 Haemophilus spp. Proteose Peptone and 15x100mm plate, 0.85% NaCl. Recommended for use in performing microbial 10/pk G141 180ml polycarbonate jar, 90ml fill, sampling, culture, and surveillance procedures for 12/pk U390 reprocessed endoscopes. 125ml polycarbonate bottle, 50ml fill, 42 16/pk U334 Phosphate Buffered Saline, pH 7.5 Phosphate Buffered Saline with Potato Dextrose Agar Used to prepare dilutions. 0.05% Tween 20 with Chlortetracycline 13x100mm polycarbonate PBS is used during For the selective cultivation tube, 1ml fill, specimen processing of fungi from cosmetics. 20/pk R201 for recovery of 15x100mm plate, 16x125mm polycarbonate bacterial endospores. 26ml deep fill, 10/pk W93 tube, 9ml fill, Recommended as a 20/pk K146 component of Potato Dextrose Agar 16x125mm polycarbonate screening programs for with Chloramphenicol tube, 7ml fill, use in nasal swab heat- For the selective cultivation 20/pk K148 shock procedures for of fungi. 16x125mm polycarbonate recovery of Bacillus 15x100mm plate, tube, 9.9ml fill, anthracis endospores. 28ml fill, 100/pk W94BX 20/pk K163 125ml polypropylene Potato Dextrose Agar with TA bottle, HDPE, 100ml fill, 125ml polypropylene bottle, (Tartaric Acid) 100ml fill, 12/pk U235 For the cultivation of Each U137 PLET Agar fungi from food and dairy 1L polycarbonate bottle, (Polymyxin Lysozyme EDTA Thallous products. 1000ml fill, Acetate) 15x100mm plate, 10/pk U138 For the selective isolation 28ml deep fill, Phosphate Buffer with of from 10/pk W96 Magnesium Chloride environmental samples, Potato Flake Agar animal products, carcasses, For preparation of dilutions For the cultivation of fungi. and clinical samples from Dilu-Lok™ Vial, 145ml Induces conidia and spore non-sterile sites. polypropylene vial, with flip-top formation. 15x100mm plate, lid, 50/pk: 15x100mm plate, 10/pk G153 90ml fill D690 26ml deep fill, 99ml fill D699 Potato Dextrose Agar, USP 10/pk W59 For the cultivation of fungi. HardyFlask™, 12ml slant, Plate 20/pk X32 15x100mm, 26ml deep fill, Dilu-Lok™ 10/pk W60 Tube 20x125mm glass, 10ml slant, 20/pk L90

Phosphate Buffer with Magnesium Chloride, Cat. no. D690.

Trichophyton mentagrophytes Trichophyton growing on Potato Dextrose Agar mentagrophytes with Chloramphenicol, (ATCC® 19533) colonies Cat. no. W94BX. growing on Potato Flake Agar, Cat no. W59.

43 Potato Flake Selective Agar For the selective isolation of Rapid Ornithine, Showing positive (right) Staphylococcus lugdunensis pathogenic fungi. (ATCC® 49576) and negative (left) Staphylococcus epidermidis (ATCC® 12228) reactions on Rapid Ornithine within 4 hours, Cat. no. K279. 15x100mm plate, 26ml deep fill, 10/pk W159 PPLO Selective Agar (Pleuropneumonia-Like Organisms) For the selective isolation of Mycoplasma and ureaplasma, especially . With amphotericin B, and penicillin, 15x60mm plate, 10/pk G04 Pseudomonas Agar F (Pseudomonas Agar Fluorescein) For the identification of Pseudomonas aeruginosa and the detection of fluorescein, a fluorescent Burkholderia (Pseudomonas) cepacia greenish-yellow pigment. (ATCC ® 25416) growing on Pseudomonas Isolation Agar, Cat. no. G145 15x100mm plate, 10/pk G198 Pseudomonas Agar P For enhancement of pyocyanin production by Pseudomonas aeruginosa. 15x100mm plate, 18ml fill, 10/pk G201 Pseudomonas aeruginosa Pseudomonas Isolation Agar growing on Pseudomonas Agar For the selective isolation of F, Cat. no. G198. Pseudomonas aeruginosa. 15x60mm plate, 10/pk G145 15x100mm plate, 10/pk G219

R10 Broth (Rappaport-Vassiliadis R10 Broth) For the selective enrichment (ATCC® 9797) of Salmonella spp. growing on Regan-Lowe Agar, 16x125 glass tube, 10ml fill, Cat. no. A65. 20/pk K167 Rapid Ornithine This test will assist in the identification of Enterobacteriaceae and Staphylococcus lugdunensis; detects ornithine decarboxylase Rappaport-Vassiliadis R10 Broth, activity in as little as two to Cat. no. K167 four hours. 15x103mm polycarbonate tube, 1ml fill, 20/pk K279

44 Regan-Lowe Agar (Charcoal Blood Agar with 10% Horse Blood and Cephalexin) For the selective isolation of Bordetella pertussis. 15x100mm plate, 10/pk A65 Regan-Lowe Semisolid (Charcoal Blood Agar with 10% Horse Blood Pseudomonas aeruginosa and Cephalexin) growing on Pseudomonas Agar A semisolid medium P, 15x100mm plate, developed specifically for Cat. no. G201. Bordetella pertussis. 13x100mm polycarbonate tube, 4ml deep fill, 20/pk Q32 Rose/MacConkey Biplate Rose Agar (Rose Agar, Selective For Gram-Positive Bacteria/ For the selective isolation of MacConkey Agar, Selective Gram-positive bacteria for Gram-Negative Bacteria), 15x100mm plate, Cat. no. J85. 10/pk A66 Rose/MacConkey Biplate (Rose Agar, Selective For Gram-Positive Bacteria/MacConkey Agar, Selective for Gram-Negative Bacteria) Section I: Rose Agar is recommended for use as a selective growth medium for the cultivation and isolation of Gram-positive cocci from clinical and non- clinical specimens which contain mixed flora. Section II: MacConkey Agar is recommended for use as a selective and Candida albicans differential medium for growing on SabDex the isolation of gram- Agar, contact plate, negative bacilli (including 15ml fill, coliform organisms and Cat. no. P36. enteric pathogens), on the basis of lactose fermentation. 15x100mm biplate, 10/pk J85 Rose Bengal Agar with Chloramphenicol For the selective isolation Trichophyton and enumeration of fungi. mentagrophytes Contact plate, (ATCC® 9533) colonies 10/pk P42 growing on Sabouraud 20x150mm glass tube, Dextrose Agar, 20ml fill, 100/pk Q81 Emmons, Cat. no. W20.

45 RTF Modified Casman Agar SabDex Agar with C SabDex Agar with Gentamicin (Rousseau, Trowbridge, Fisher) and G/DTM Biplate and Chloramphenicol For the cultivation and (Sabouraud Dextrose Agar with (Sabouraud Dextrose) differentiation of fastidious Chloramphenicol and Gentamicin/ For the selective cultivation microorganisms, especially Dermatophyte Test Medium) of fungi and dermatophytes. Haemophilus spp. by Section I: Sabdex Agar with C 15x60mm plate, hemolytic reactions. and G are recommended 10/pk G159 15x100mm plate, for the isolation, cultiva- 15x100mm plate, 10/pk A68 tion, and maintenance 26ml deep fill, RV Broth, USP of non-pathogenic and 10/pk W73 (Rappaport-Vassiliadis Broth) pathogenic species of SabDex Agar with Lecithin For the selective enrichment fungi and yeasts. and Tween® 80 Section II: DTM is a selective of Salmonella spp. (Sabouraud Dextrose) 16x125mm glass tube, and differential medium recommended for the For the isolation and 10ml fill, cultivation of fungi and 20/pk K246 cultivation and isolation of pathogenic dermato- the neutralization of disinfectants. SabDex Agar phytic fungi. 500ml polypropylene bottle, (Sabouraud Dextrose) 15x100mm biplate, 400ml fill, For the cultivation of fungi. 10/pk J107 USP, Plate 10/pk U175 SterEM™, 15x100mm plate, SabDex Agar 15x100mm plate, 18ml fill, irradiated, with Chloramphenicol 26ml deep fill, triple bagged, (Sabouraud Dextrose Agar with 10/pk W71 Contact plate, 10/pk W1770 Chloramphenicol) Plate For the selective isolation 10/pk P46 USP, 15x100mm plate, of fungi. SabDex Agar with Lecithin and 26ml deep fill, 15x100mm plate, Tween® 80, SterEM™, Irradiated 26ml deep fill, 10/pk W70 (Sabouraud Dextrose) 10/pk Contact plate, W72 For the cultivation and 10/pk P36 HardyFlask™, 12ml slant, enumeration of fungi and for Glass tube 20/pk X41 environmental monitoring. 20x125mm, 10ml slant, SabDex Agar with Neutralizes disinfectants. 20/pk L40 Chloramphenicol and 15x100mm plate, 34ml fill, USP, 20x125mm, 18ml deep triple bagged, Tetracycline 10/pk W257 fill, 20/pk Q31 (Sabouraud Dextrose) USP, 20x150mm, 20ml For the selective isolation deep fill, 100/pk Q83 of fungi. USP, 500ml, 500ml fill, 15x100mm plate, 10/pk U353 26ml deep fill, Flask 10/pk W74 HardyFlask™, 12ml slant, 20/pk X40 SabDex Agar, Emmons (Sabouraud Dextrose) For the cultivation of fungi. 15x100mm plate, 26ml deep fill, 10/pk W20 HardyFlask™, 10ml fill, 20/pk X57

SabDex Broth, Tube, 16x125mm, 10ml fill, Cat. no. K54. 46 Sabdex Agar with Lecithin and Tween® 80, contact plate, Cat. no. P46.

Candida albicans growing on SabHI Agar with Blood, SabDex Agar with Chloramphenicol Chloramphenicol, and and Tetracycline, Cat. no. W74. Cycloheximide HardyFlask™, Cat. no. X73.

Trichophyton mentagrophytes (ATCC® 9533) colonies growing on Sabouraud Dextrose Saline, 0.85% Agar, Emmons, For the preparation of Cat. no. W20. isotonic suspensions and dilutions of microorganisms. Glass tube 13x100mm, 2ml fill, SabHI Agar with Blood, SabDex Broth 20/pk R55 (Sabouraud Dextrose) Chloramphenicol, and 16x100mm, 5ml fill, For the cultivation of fungi. Cycloheximide 20/pk K59 Glass tube (Sabouraud-Brain Heart Infusion) 16x100mm, 9ml fill, 16x125mm, 10ml fill, HardyFlask™, 12ml fill, 20/pk K52 20/pk K54 20x150mm, 10ml fill, 20/pk X73 USP, polycarbonate bottle 20/pk K58 125ml, 100ml fill, Saline, 0.45% Polycarbonate tube For the preparation of 16/pk U73 13x100mm, 2ml fill, isotonic suspensions and SabHI Agar dilutions of microorganisms. 20/pk R45 (Sabouraud-Brain Heart Infusion) 11.75x75mm glass tube, 13x100mm, 4ml fill, For the cultivation of fungi. 1.8ml fill, 20/pk R47 HardyFlask™, 12ml fill, 100/pk D12 Polypropylene bottle 20/pk X75 125ml, 100ml fill, 12/pk U155 1L, 1000ml fill, 10/pk U157

47 Saline, 0.9% with 0.5% Tween® 80 For preparing dilutions and suspensions of microorganisms. 16x125mm glass tube, 9ml fill, 20/pk K123 Selective Beta Strep Agar, Group A Group A Beta Strep Agar is an enriched media for the selective isolation of Group A streptococci (Streptococcus pyogenes). 15x100mm plate, 10/pk A72 Streptococcus pyogenes growing on Selective Beta Strep Agar, Group A, Cat. no. A72. Selective Strep Agar (COBA Medium) (Colistin, Oxolinic Acid in a Blood Agar Base) Recommended for use in the primary isolation of all Streptococcus species, including streptococcal Groups A (Staphylococcus pyogenes), B (Streptococcus agalactiae), C, D, F, G, and Streptococcus pneumoniae, especially from Streptococcus pneumoniae (ATCC® 6305) respiratory specimens. colonies growing on Selective Strep Agar, Cat. no. A70. 15x100mm plate, 10/pk A70 Selenite Cystine Broth For the selective enrichment of Salmonella spp. 16x125mm glass tube, 10ml fill, 20/pk K69

SIM Medium (Sulfide-Indole-Motility) For the identification of Gram-negative bacilli based on sulfide production, indole formation, and motility. 16x100mm glass tube, Salmonella enterica growing on 8ml deep fill, SS Agar, 15x100mm plate, 20/pk Cat. no. G50. Q30 48 Salmonella enterica (ATCCC® 14028) colonies growing on Tergitol 7 Agar with TTC, Cat. no. G58. Simmons Citrate Agar For the detection of citrate Vibrio parahaemolyticus (ATCC® 17802) utilization by Gram-negative colonies growing on TCBS Agar, enteric bacilli. Cat. no. G55. 16x100mm glass tube, 5.5ml slant, 20/pk L80 Pseudomonas aeruginosa (ATCC® 27853) growing on Skim Milk Agar, Skim Milk Agar With Urea Cat. no. G138. For the cultivation and dif- 13x100mm polycarbonate ferentiation of microorgan- tube, 2ml fill, isms based on proteolytic 20/pk R87 activity. SS Agar 15x60mm plate, Sterile Deionized Water 10/pk G138 (Salmonella Shigella) Sterile deionized water For the isolation of SP4 Agar lacks ions such as sodium, pathogenic enteric bacteria, For the selective cultivation calcium, iron, and copper. especially Salmonella and and differentiation of Useful for general laboratory Shigella spp. Mycoplasma spp. use and for the preparation 15x100mm plate, With Arginine of dilutions and suspensions. 10/pk 15x60mm plate, G50 13x100mm tube, 5ml fill, 10/pk G32 Standard Methods Agar 20/pk K187 (/TGY [Tryptone Glucose 125ml polypropylene bottle, SP4 Broth 100ml fill, For the selective Yeast] Agar) For the cultivation 12/pk U85 cultivation and differentia- 1L polycarbonate bottle, tion of mycoplasma includ- and enumeration of microorganisms. 1000ml fill, 10/pk ing Mycoplasma hominis, U284 Mycoplasma pneumoniae, Glass tube and . 20x125mm, 18ml deep fill, Sterile Water 20/pk Q21 With Arginine For use in performing 13x100mm polycarbonate Plate microbial sampling, tube, 2ml fill, 15x100mm, 10/pk G43 culture, and surveillance procedures for reprocessed 20/pk R85 Glass bottle With Glucose 118ml glass, 100ml fill, endoscopes. 125ml polypropylene bottle, 20/pk U95 125ml polycarbonate bottle, 90ml fill, 500ml glass, 400ml fill, 50ml fill, 16/pk Each U86 12/pk U395 U336 49 ™ Strep B Carrot Broth One-Step Strep B Carrot Broth™ One-Step is a selective and differential medium for the detection of Group B Streptococcus (GBS) from anovaginal specimens collected from pregnant women. The medium is used as an aid in the qualitative determination of GBS colonization in pregnant women. The color change reaction from white to orange is representative of a positive result for presence of hemolytic GBS.

Our one-step test is an improvement over conventional methods by increasing sensitivity, decreasing turn-around time, and lowering overall cost. All components are now included in the color change test, so the use of additional tiles is no longer necessary. Positives require no follow up testing! • Easy one-step process! Place sample directly in the tube. Adding tiles is no longer necessary. • Easy read-out. Development of any orange to red color whatsoever, indicates a positive result. • For positives, there is no need for further sub- culturing or testing. • Lower cost when compared to the LIM broth method. • Can be used with liquid and gel-based transport systems. • Carrot Broth media is available in multiple formats that work on many automated inoculating machines, such as the WASP® or BD Innova® 12x80mm plastic tube, compatible with the WASP® System, 100/pk Z44BX 13x100mm tube, 4ml fill, 20/pk Z40 16x100mm tube, 6ml fill, compatible with BD Innova® 100/pk Z46BX (See GBS Detect Plate, page 25)

50 Trehalose Broth, Rapid, showing positive results for Candida glabrata on the left, Cat. no. Z205.

Standard Methods Agar, Cat. no. Q21.

Vibrio parahaemolyticus (ATCC® 17802) colonies growing on TCBS Agar, Cat. no. G55. TAT Broth ® (Tryptone-Azolectin-Tween ) For use in the cultivation of microorganisms from highly viscous or gelatinous materials. 20x125mm glass tube, 9ml fill, TCBS Agar 20/pk K251 (Thiosulfate Citrate Bile Sucrose) 125ml boston round glass For the selective Tryptic Soy Agar, bottle, 90ml fill, isolation of Vibrio USP, tube, 20ml 20/pk U87 and deep fill, 125ml boston round glass Vibrio Cat. no. Q85. bottle, 99ml fill, parahaemolyticus. 20/pk U78 15x100mm plate, 236ml wide mouth glass jar, 10/pk G55 90ml fill, (See HardyCHROM™ Vibrio, page 30) 12/pk U77 Tergitol 7 Agar with TTC 236ml wide mouth glass jar, For the isolation and 99ml fill, differentiation of Gram- 12/pk U88 Tetrathionate Broth with negative bacilli, contains triphenyltetrazolium Brilliant Green TB Base Digestant chloride. For the selective For processing specimens 15x100mm plate, enrichment of Salmonella spp., use for AFB culture; add NALC, 10/pk G58 Cat. no. Z60, upon use. with iodine-iodide solution, Cat. no. Z129. Bottle Tetrathionate Broth 60ml polypropylene, 16x125mm glass tube, For selective enrichment of 50ml fill, 10ml fill, 20/pk K164 Salmonella spp., use 25/pk X45 with iodine-iodide solution, 125ml polypropylene, Thayer Martin Agar Cat. no. Z129. 100ml fill, For the selective 16x125mm glass tube, 12/pk U22 isolation of Neisseria 10ml fill, 500ml polypropylene, gonorrhoeae; contains 20/pk K65 500ml fill, vancomycin, colistin, 1L polycarbonate bottle, Each U20 and nystatin. 1000ml fill, 15x100mm plate, 10/pk U165 100/pk E130BX 51 Thayer Martin Agar, Modified Trehalose Broth, Rapid 15x100mm plate, 26ml deep For the selective Carbon Assimilation Test fill, triple bagged, isolation of Neisseria gon- for the identification of 10/pk W1670 orrhoeae; contains vanco- yeasts, especially Candida 15x100mm plate, 26ml fill, mycin, colistin, nystatin, and (Torulopsis) glabrata. with Lecithin and Tween® 80 trimethoprim. 13x100mm polycarbonate double-bagged, 15x100mm plate, tube, 0.35ml fill, 10/pk W2530 10/pk E30 20/pk Z205 15x100mm plate, 34ml deep fill, triple bagged, Thioglycollate with H and K Tryptic Soy Agar 10/pk W3670 (Hemin and Vitamin K) (TSA) 15x100mm, red tinted For the cultivation of For the cultivation of plate, 34ml deep fill, triple anaerobic bacteria, filtered microorganisms, also known bagged, to remove dead bacteria. as TSA or Soybean-Casein 10/pk W3770R Glass tube Digest Agar. 16x100mm, 5ml fill, TSA with Cycloheximide USP, plate 20/pk K22 15x100mm, (Tryptic Soy Agar) 16x125mm, 10ml fill, For general growth for the 10/pk G60 20/pk isolation and cultivation K24 15x100mm, of microorganisms while 100/pk Thioglycollate without Indicator G60BX inhibiting Saprophytic fungi. 15x100mm, 26ml deep fill, For the cultivation of 15x100mm plate, 10/pk microorganisms filtered to W64 10/pk G70 15x150mm, remove dead bacteria TSA with Lecithin and Tween® 80 16x125mm glass tube, 10/pk H19 10ml fill, without plate label, orientation (Tryptic Soy Agar) tabs, and logo For the cultivation and enu- 20/pk K29 15x100mm plate, meration of microorganisms, Thioglycollate without Indicator, 10/pk G62 neutralizes disinfectants. Glass tube Lok-Tight™ Contact Plates with Supplements 16x100mm, 5.5ml slant, 10/pk P34 For the cultivation of 20/pk L60 Irradiated, USP, 15ml fill, fastidious anaerobes; 20x150mm, 20ml deep fill, triple bagged, contains hemin, vitamin K, 100/pk Q85 and a calcium carbonate 10/pk P520 USP, glass tube Irradiated, USP, red tinted, chip as a buffer; filtered to 20x125mm, 18ml deep fill, remove dead bacteria. 15ml fill, triple bagged, 20/pk Q58 10/pk P520R 16x125mm glass tube, USP, bottle 10ml fill, 20/pk USP, Plate K23 250ml glass, 150ml fill, USP, 15x100mm, Thioglycollate with Indicator 20/pk U49 250ml glass,200ml fill, 10/pk G41 (See Fluid Thioglycollate, page 24) 12/pk U260 Plate Todd Hewitt Broth 500ml glass, 400ml fill, 15x100mm, 27ml deep fill, 10/pk For the cultivation of 12/pk U360 W41 Bottle Streptococcus spp. 500ml polycarbonate, 500ml polycarbonate, 15X103mm polycarbonate 500ml fill, 10/pk U361 400ml fill, tube, 5ml fill, 1L polycarbonate, 10/pk 20/pk K79 700ml fill, 10/pk U60 U174 500ml boston round glass TSA, SterEM™, Irradiated, USP bottle, 400ml fill, Todd Hewitt Broth with SXT (Tryptic Soy Agar) (Sulfamethoxazole with Trimethoprim) 12/pk U412 For general growth medium For the selective isolation of for the detection and enu- Group A strep. meration of microorganisms 16x100mm polycarbonate from environmental sources. tube, 3ml fill, 15x100mm plate, 20/pk K113 triple bagged, 2/pk G267

52 TSA Blood/CLED/MAC Glass bottle, needle port TSB Tubes (Tryptic Soy Agar with 5% Blood/ 500ml, 500ml fill, (Tryptic Soy Broth) Cystine-Lactose-Electrolyte-Deficient/ 10/pk U274 For use as a general purpose MacConkey Agar) Glass bottle medium for the isolation and Section I: Blood Agar prod- 60ml boston round, cultivation of a wide variety of bacteria and fungi. ucts are used as general 50ml fill, 24/pk U44 USP, Glass tube purpose growth media 125ml boston round, for the isolation, cultiva- 16x125mm, 10ml fill, 100ml fill, 20/pk U42 tion, and differentiation of 20/pk K82 microorganisms. Polycarbonate bottle 16x125mm, 10ml fill, 80 tubes Section II: CLED Agar is 125ml, 100ml fill, with needle in ReadyRack™ K82BX used for the isolation, port cap, 16/pk U141 20x125mm, 15ml fill, 250ml, square bottom, enumeration, and pre- 20/pk K83 sumptive identification of 160ml fill, 12/pk U171 20x150mm, 20ml fill, 500ml, 500ml fill, Gram-positive organisms 100/pk K380 on the basis of lactose Each U65 fermentation, while con- 1L, 1000ml fill, Glass tube trolling the swarming of 10/pk U67 13x100mm, 2ml fill, Proteus spp. Polycarbonate jar 20/pk R31 Section III: MacConkey Agar 180ml, wide mouth, 90ml fill, 13x100mm, 5ml fill, 20/pk R30 is used as a selective and 12/pk U133 16x100mm, 9ml fill, differential medium for the 180ml, wide mouth, 20/pk K88 isolation of urinary patho- 100ml fill, gens bacilli, on the basis of 12/pk U71 Polycarbonate tube lactose fermentation. 13x100mm, 1ml fill, 15x100mm triplate, TSB, Double Strength, USP 20/pk 10/pk R36 J315 (Tryptic Soy Broth) 13x100mm, 3ml fill, TSA (Tryptic Soy Agar) with For the cultivation of 20/pk R41 Lecithin and Tween® 80, USP microorganisms. 15x103mm, 2ml fill, For the cultivation and enu- USP 20/pk K131 meration of microorganisms. 180ml wide mouth 16x100mm, 5ml fill, Irradiated, triple bagged, polycarbonate jar, 50ml fill, 20/pk K89 10/pk P870 12/pk U205 15x103mm, filtered, 1ml fill, 250ml glass bottle, 20/pk K85 TSB, USP, Bottles 100ml fill, 15x103mm, filtered, 2ml fill, 12/pk (Tryptic Soy Broth) U70 20/pk K285 For use as a general purpose Bottle With 15% Glycerol medium for the isolation and 118ml glass, 25ml fill, 13x100mm polycarbonate cultivation of a wide variety 12/pk U225 tube, 2ml fill, of bacteria and fungi. 20/pk R34 Glass serum vial, needle port, crimp cap 20ml, 15ml fill, 50/pk U82 20ml, 20ml fill, 50/pk U38 20ml, 10ml fill, 50/pk U8210 100ml, 50ml fill, 20/pk U267 100ml, 100ml fill, Tryptic Soy Broth, USP, 20/pk U46 with needle port sept Cat. no. U46.

53 TSB with 6.5% Sodium Chloride Tryptone Glucose Extract Agar Wallenstein Media (Tryptic Soy Broth) For the cultivation and For the cultivation of For the differentiation of enumeration of bacteria. Mycobacterium spp. Enterococcus spp. from 15x100mm plate, especially Mycobacteria Group D streptococci. 10/pk G115 avium complex. 15X103mm polycarbonate 20x125mm glass tube, tube, 5ml fill, Urea (Christensen’s Urea) 10ml slant, 20/pk K126 Agar Slant 20/pk C61 For the detection of urease ® TSB with Lecithin and production in bacteria. Water with Tween 80 ® ® Tween 20, USP 13x100mm glass tube, (Deionized water with 0.02% Tween 80) (Tryptic Soy Broth) 3ml slant, For preparing dilutions. For the cultivation of 20/pk R42 20x125mm glass tube, microorganisms. 16x100mm glass tube, 24ml fill, 240ml wide mouth jar, 5.5ml slant, 20/pk V25 90ml fill, 20/pk L65 XLD Agar 12/pk U241 Urea Medium, Rapid (Xylose Lysine Deoxycholate) 240ml wide mouth jar, For the rapid determination For selective isolation of 100ml fill, of urease activity. pathogenic, enteric, Gram- 12/pk U261 15x45mm glass tube, negative bacteria. TSB with Lecithin and 2ml fill, 15x100mm plate, Tween® 80 10/pk Z54 10/pk G65 (Tryptic Soy Broth) UVM Modified Listeria Broth XLT4 Agar For the cultivation of (Univesity of Vermont) (Xylose Lysine Tergitol 4) microorganisms. For the selective cultivation For the selective isolation of 20x125mm glass tube, of Listeria monocytogenes. non-typhi Salmonella spp. 9ml fill, 500ml polycarbonate 15x60mm plate, 20/pk K134 bottle, 225ml fill, 10/pk G277 500ml polycarbonate bottle, 10/pk U153 15x100mm plate, 500ml fill, 10/pk G165 10/pk U229 V9 Agar 236ml glass, USP, wide (V8™ Juice and Potato Flakes) XLT4 Agar/Brilliant Green Agar mouth jar, 90ml fill, For the cultivation of fungi. with Sulfadiazine Biplate 12/pk Induces sporulation. U134G Section I: XLT-4 Agar is a 15x100mm plate, highly selective plating TSI Agar 22ml deep fill, medium for the detection 10/pk (Triple Sugar Iron) G98 and isolation of non-typhi For the differentiation of Vogel and Johnson Agar Salmonella species. Gram-negative bacilli. For the selective isolation Section II: Brilliant Green 13x100mm glass tube, of coagulase-positive, Agar with Sulfadiazine 4ml slant, mannitol-positive is recommended for the 20/pk R32 staphylococci. isolation of Salmonella 16x125mm glass tube, 15x100mm plate, spp., other than 8ml slant, 10/pk G193 Salmonella typhi and 20/pk L50 Salmonella paratyphi, from VRBA Tryptone Broth, 2% food samples, especially (Violet Red Bile Agar) eggs, following an For detecting indole For the detection and enrichment procedure. production, use with Kovac’s enumeration of coliforms. 15x100mm biplate, Reagent, Cat. no. Z67. 15x100mm plate, 10/pk 13x100mm polycarbonate J131 10/pk (See HardyCHROM™ Salmonella/XLT-4 Agar tube, 2ml fill, G78 Biplate, page 28) 20/pk R40 VRBGA (Violet Red Bile Agar with Glucose) For the detection of Enterobacteriaceae. 15x100mm plate,

54 10/pk G178 Surface Sampling

Contact Plates Surface sampling shall be performed in all ISO classified areas on a periodic basis per USP <797>

TSA (Tryptic Soy Agar) with Lecithin and Tween® 80 FINGERTIP For the cultivation and enumeration SAMPLING of microorganisms. 1. Twist and remove lid. 10/pk P34 SabDex (Sabouraud Dextrose) Agar For the cultivation of fungi. 10/pk P36 MEA (Malt Extract Agar) with Lecithin and Tween 1. Twist and remove lid. For the cultivation and 2. Gently press plate to surface without enumeration of fungi. twisting or sliding. 10/pk P93

IRRADIATED CONTACT PLATES TSA (Tryptic Soy Agar) with 2. Gently press glove tips to Lecithin and Tween® 80, USP plate surface. 3. Twist clockwise until frosted bands For the cultivation line up and lock lids into place. Incubate and enumeration of at 30 oC - 35 oC. After 48 - 72 hours, microorganisms. count the number of colonies. Record Irradiated, triple bagged, your results. 10/pk P520 Red tinted plate, triple bagged, 10/pk P520R SabDex (Sabouraud Twist lid clockwise until frosted bands line up and lid locks into o o Dextrose) Agar with place. Incubate at 30 C - 35 C. After Lecithin & Tween® 80, USP 48 - 72 hours, count the number of For the cultivation of fungi. colonies. Record your results. Friction Lid design keeps lid in place. Irradiated, triple bagged, Locking feature assures lids stay secured. 10/pk P595 55 Media Paddles COME IN 2 FORMATS Tryptic Soy Agar (TSA) or Malt Extract Agar (MEA)

Tryptic Soy Agar (TSA) Media-Paddles Support the growth of a wide range of organisms. TSA Media-Paddles are often used for environmental and cleanroom monitoring for bacterial and fungal contamination.

Malt Extract Agar (MEA) Media-Paddles Specific for fungal growth, a general purpose agar that reduces bacterial growth. Tryptic Soy Agar (TSA), Features and Benefits FlexEM™ Media Paddles 10/pk TSA10 ``Recessed handle built into the slide Malt Extract Agar (MEA), FlexEM™ Media Paddles 10/pk MEA10 Packaged in a Clean Environment to Reduce Particulates ``Flexible hinged paddle that can FINGERTIP be angled up to 90+ degrees in SAMPLING each direction

``Identifier on the side - allows agar identification

``Dual sided - same agar on both FlexEM™ Media Paddle, sides of the paddle Cat. no. TSA10 can also be used for fingertip sampling. ``Quality tested for performance, pH

56 and sterility TO BRING MEDIA INTO A CONTROLLED AREA A CONVENIENT WAY WHILE AVOIDING OUTSIDE CONTAMINATION.

80 tube ReadyRack™

Holes in the bottom of the rack allow for sterilant to drain Remove ReadyRack™ from box outside of cleanroom.

Packaged in a Clean Environment to Reduce Particulates

Tryptic Soy Broth (TSB), USP Take into cleanroom 16x125mm glass tube, 10ml fill, then remove plastic bag. 80/pk K82BX Fluid Thioglycollate Medium (FTM), USP 16x125mm glass tube, 10ml fill, 80/pk K212BX Other tube formats and fill volumes are available upon request. Call (800) 266-2222 ext. 5696

57 1430 West McCoy Lane Santa Maria, CA 93455 p: 800.266.2222 www.HardyDiagnostics.com [email protected]