Prepared Culture Media
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Insight Into the Resistome and Quorum Sensing System of a Divergent Acinetobacter Pittii Isolate from 1 an Untouched Site Of
bioRxiv preprint doi: https://doi.org/10.1101/745182; this version posted November 27, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Insight into the resistome and quorum sensing system of a divergent Acinetobacter pittii isolate from 2 an untouched site of the Lechuguilla Cave 3 4 Han Ming Gan1,2,3*, Peter Wengert4 , Hazel A. Barton5, André O. Hudson4 and Michael A. Savka4 5 1 Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong 6 3220 ,Victoria, Australia 7 2 Deakin Genomics Centre, Deakin University, Geelong 3220 ,Victoria, Australia 8 3 School of Science, Monash University Malaysia, Bandar Sunway, 47500 Petaling Jaya, Selangor, 9 Malaysia 10 4 Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, USA 11 5 Department of Biology, University of Akron, Akron, Ohio, USA 12 *Corresponding author 13 Name: Han Ming Gan 14 Email: [email protected] 15 Key words 16 Acinetobacter, quorum sensing, antibiotic resistance 17 18 Abstract 19 Acinetobacter are Gram-negative bacteria belonging to the sub-phyla Gammaproteobacteria, commonly 20 associated with soils, animal feeds and water. Some members of the Acinetobacter have been 21 implicated in hospital-acquired infections, with broad-spectrum antibiotic resistance. Here we report the 22 whole genome sequence of LC510, an Acinetobacter species isolated from deep within a pristine 23 location of the Lechuguilla Cave. -
Enterobacteriaceae Family (CRE), Is of Utmost Importance for the Enterobacteriaceae Management of Infected Or Colonized Patients
2013 iMedPub Journals THE INTERNATIONAL ARABIC JOURNAL Vol. 3 No. 3:5 Our Site: http://www.imedpub.com/ OF ANTIMICROBIAL AGENTS doi: 10.3823/737 Evaluation of Rula Al-Dawodi1,3, Rawan Liddawi1,3, Raed Ghneim1,3, Randa Kattan1,3, Issa Siryani1,3, Afaf Abu-Diab1, Riyad Meropenem, Ghneim1, Madeleine Zoughbi1,3, Abed-El-Razeq Issa1,3, Randa Al Qass1,3, Sultan Turkuman1, Hiyam Marzouqa1, and Imipenem and Musa Hindiyeh1,2,3* Ertapenem 1 Caritas Baby Hospital, 3 Palestinian Forum for Medical * Correspondence: Bethlehem, Palestine; Research (PFMR), Ramallah, Impregnated 2 Bethlehem University, Palestine Bethlehem, Palestine; [email protected] MacConkey * Musa Y Hindiyeh, Caritas Baby Hospital Bethlehem Agar Plates for Palestine. the Detection of Carbapenem Abstract Resistant Background: Rapid detection of carbapenem resistant bacteria, in particular, members of the Enterobacteriaceae family (CRE), is of utmost importance for the Enterobacteriaceae management of infected or colonized patients. Methods: Three carbapenems; meropenem, imipenem and ertapenem, with two different concentrations (0.5 mg/ml and 1.0 mg/ml), were impregnated in Mac- Conkey agar. The carbapenem impregnated MacConkey agar plates; ([Mac-Mem], [Mac-Imp] and [Mac-Ert]), were then evaluated for the detection of carbapenem resistant Gram-negative bacteria in particular the blaKPC producing Enterobacteria- ceae. The Limit of Detection (LOD) of the plates was determined in triplicate after serial logarithmic dilution of the bacterial strains in saline. This was followed by inoculating the plates and counting the colonies that grew after 24 hours of incu- bation. The specificity and the shelf-life of the plates were determined by testing the plates with six Extended Spectrum β-lactamases (ESBL) producing members of the Enterobacteriaceae family and one genus with the blaAmpC phenotype. -
Dehydrated Culture Media
Dehydrated Culture Media Manufactured by Dehydrated Culture Media Table of Contents 4 CRITERION™ Products 12 Supplements and Antibiotics 13 CRITERION™ Agarose for Gel Electrophoresis Dehydrated Culture Media ™ TM Hardy Diagnostics’ dehydrated culture media, CRITERION , is formulated to meet or exceed the highest quality standards. DEHYDRATED CULTURE MEDIA Choose from 250 standard formulas or request custom blending to your specifications. The innovative packaging designs and overall reliability makeCRITERION ™ the logical choice for culture media in your laboratory. FEATURES & BENEFITS Hand Grip Convenient hand-grip design features finger indentations to allow for easy and safe handling of the bottle. Induction Seal Gray Jar Pull-off induction seal prevents moisture from clumping the media, keeping it fresh and dry. Opaque gray jar diminishes Wide Mouth Opening light penetration, • Allows for easy access to use a scoop when prolonging superior measuring the powder. performance and • Prevents inhalation hazards and reduces shelf life. hazardous dust formations. • No more shaking the bottle to dispense the media. Desiccant Pack A silica gel pack is included in each bottle to prevent clumping. Reusable Seal A built-in cushion seal inside the lid prevents moisture from entering the previously opened container. 1 UNPARALLELED PERFORMANCE Every formulation and lot is thoroughly tested for optimal growth characteristics. WIDE MOUTH OPENING Scooping media from the wide mouth bottle, instead of pouring and shaking, reduces dangerous dust formation CONVENIENT SIZES Packaged in four standard sizes to fit your needs: • 2 liter Mylar® bag (pre-measured to make 2 liters of culture media) • 500gm bottle • 2kg buckets with locking screw top lid • 10kg buckets with locking screw top lid STACKABLE Bottles and buckets have a nesting design and are stackable for efficient and economical storage. -
Bile Esculin Agar
BILE ESCULIN AGAR INTENDED USE Remel Bile Esculin Agar is a solid medium recommended for use in qualitative procedures for the presumptive identification of group D streptococci and enterococci. SUMMARY AND EXPLANATION Rochaix first demonstrated the value of esculin hydrolysis for identification of enterococci.1 Meyer et al. found that 61 of 62 strains of enterococci hydrolyzed esculin in a medium containing bile.2 Swan determined that positive results obtained on esculin agar containing 40% bile correlated well with serologically confirmed group D streptococci.3 Using Swan’s formula, Facklam and Moody tested over 700 strains of streptococci and enterococci representing all known serological groups and found all strains to be bile-resistant and esculin-positive. PRINCIPLE Group D streptococci and enterococci hydrolyze esculin in the presence of bile to form esculetin and dextrose. Esculetin reacts with ferric ions supplied by ferric ammonium citrate to form brown-black colonies on Bile Esculin Agar. Oxgall in a concentration of 4% (equivalent to 40% bile) inhibits most strains of streptococci and enterococci other than group D. REAGENTS (CLASSICAL FORMULA)* Oxgall (40% Bile) ............................................................ 40.0 g Esculin...............................................................................1.0 g Gelatin Peptone ................................................................ 5.0 g Ferric Ammonium Citrate...................................................0.5 g Beef Extract....................................................................... 3.0 g Agar.................................................................................15.0 g Demineralized Water...................................................1000.0 ml pH 6.8 ± 0.2 @ 25°C *Adjusted as required to meet performance standards. PRECAUTIONS This product is For Laboratory Use only. It is not intended for use in the diagnosis of disease or other conditions. PREPARATION OF DEHYDRATED CULTURE MEDIUM 1. Suspend 64 g of medium in 1000 ml of demineralized water. -
Evaluating Historical Paradigms of Sterility in Perinatal Microbiology and Ramifications Orf Pregnancy Outcomes
Wayne State University Wayne State University Dissertations January 2020 Evaluating Historical Paradigms Of Sterility In Perinatal Microbiology And Ramifications orF Pregnancy Outcomes Jonathan Greenberg Wayne State University Follow this and additional works at: https://digitalcommons.wayne.edu/oa_dissertations Part of the Microbiology Commons, and the Obstetrics and Gynecology Commons Recommended Citation Greenberg, Jonathan, "Evaluating Historical Paradigms Of Sterility In Perinatal Microbiology And Ramifications orF Pregnancy Outcomes" (2020). Wayne State University Dissertations. 2468. https://digitalcommons.wayne.edu/oa_dissertations/2468 This Open Access Dissertation is brought to you for free and open access by DigitalCommons@WayneState. It has been accepted for inclusion in Wayne State University Dissertations by an authorized administrator of DigitalCommons@WayneState. EVALUATING HISTORICAL PARADIGMS OF STERILITY IN PERINATAL MICROBIOLOGY AND RAMIFICATIONS FOR PREGNANCY OUTCOMES by JONATHAN MURRAY GREENBERG DISSERTATION Submitted to the Graduate School of Wayne State University, Detroit, Michigan in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY 2020 MAJOR: BIOCHEMISTRY, MICROBIOLOGY, & IMMUNOLOGY Approved by: Advisor Date DEDICATION This dissertation is dedicated to my parents and sister, who have all been immeasurably supportive over my graduate career and more importantly my entire life. I could not have done this without you. ii ACKNOWLEDGMENTS There is no way for me to express fully my sincere gratitude to all the individuals who have had an impact on my life and throughout my time here at Wayne State. None of it would have been possible without Dr. Kevin Theis. Rotating in his lab was one of the best decisions of my life and quite literally got me to where I am. -
Macconkey Agar, CS, Product Information
MacCONKEY AGAR, CS (7391) Intended Use MacConkey Agar, CS is used for the isolation and differentiation of Gram-negative enteric bacilli from specimens containing swarming strains of Proteus spp. in a laboratory setting. MacConkey Agar, CS is not intended for use in the diagnosis of disease or other conditions in humans Product Summary and Explanation MacConkey Agar is based on the bile salt-neutral red-lactose agar of MacConkey.1 The original MacConkey medium was used to differentiate strains of Salmonella typhosa from members of the coliform group. Formula modifications improved growth of Shigella and Salmonella strains. These modifications include the addition of 0.5% sodium chloride, decreased agar content, altered bile salts, and neutral red concentrations. The formula modifications improved differential reactions between enteric pathogens and coliforms. MacConkey Agar, CS (“Controlled Swarming”) contains carefully selected raw materials to reduce swarming of Proteus spp., which could cause difficulty in isolating and enumerating other Gram-negative bacilli. Principles of the Procedure Enzymatic Digest of Gelatin, Enzymatic Digest of Casein, and Enzymatic Digest of Animal Tissue are the nitrogen and vitamin sources in MacConkey Agar, CS. Lactose is the fermentable carbohydrate. During Lactose fermentation a local pH drop around the colony causes a color change in the pH indicator, Neutral Red, and bile precipitation. Bile Salts and Crystal Violet are the selective agents, inhibiting Gram-positive cocci and allowing Gram-negative organisms to grow. Sodium Chloride maintains the osmotic environment. Agar is the solidifying agent. Formula / Liter Enzymatic Digest of Gelatin .................................................... 17 g Enzymatic Digest of Casein ................................................... 1.5 g Enzymatic Digest of Animal Tissue....................................... -
Bile Esculin Agar Intended Use Bile Esculin Agar Is A
Bile Esculin Agar Intended Use Bile Esculin Agar is a differential medium used for isolation and presumptive identification of group D Streptococci / Enterococci from food samples. Summary Swan formulated Bile Esculin Agar for the isolation and identification of group D Streptococci from foods. Originally, Bile Esculin Test was used for the identification of Enterococci. However, since group D Streptococci share the test with Enterococci, it is advisable that other tests such as salt tolerance be performed while identifying Enterococci. Meyer and Schonfeld showed that majority of Enterococci were able to grow in esculin and split it, while other Streptococci could not. This medium is used to differentiate Enterococci and Streptococcus bovis from other Streptococci. Principle Oxgall inhibits gram-positive bacteria other than group D Streptococci / Enterococci. Ferric citrate is an indicator of esculin hydrolysis and resulting esculetin formation. Enterococci / group D Streptococci hydrolyze the glycoside esculin to esculetin and dextrose. Esculetin reacts with ferric citrate producing brownish black complex. This medium is also shown to aid differentiation of genus Klebsiella-Enterobacter-Serratia from other Enterobacteriaceae on the basis of esculin hydrolysis. Formula* Ingredients g/L Pancreatic Digest of Gelatin 5.0 Beef Extract 3.0 Oxgall 40.0 Ferric Citrate 0.5 Esculin 1.0 Agar 15.0 Final pH (at 25°C) 6.6 ± 0.2 *Adjusted to suit performance parameters Storage and Stability Store dehydrated medium below 30°C in tightly closed container and the prepared medium at 2ºC-8°C. Avoid freezing and overheating. Use before expiry date on the label. Once opened keep powdered medium closed to avoid hydration. -
Get Consistent and Reliable Results
Need help selecting? M Microbiology Free Tech Support 800-323-4340 Cole-Parmer® Dehydrated Culture Media Get consistent and reliable results – Use when ready or dehydrated culture extends shelf life – Individually numbered with certificate of compliance expiration date, and shelf-life guarantee – Quick dissolve for faster processing – For laboratory use only Media, Dehydrated Media, Media Description Size (g) Catalog number Price Agar, bacteriological This clear gel agar is high grade and manufactured using the ice method 500 GH-14200-10 Azide dextrose broth Use for detection of fecal Streptococci in water and sewage 500 GH-14202-00 Baird parker agar Use for the selective isolation of coagulase positive Staphylococci in food 500 GH-14202-01 Blood agar base with low pH Use to cultivate a wide variety of bacteria 500 GH-14202-02 This is used as a differential medium in the isolation and presumptive identification of Bile Esculin agar 500 GH-14202-03 enterococci/group D streptococci Brain heart infusion agar Use to cultivate a wide spectrum of bacteria, yeasts, and molds 500 GH-14200-12 Brain heart infusion broth Use to cultivate fastidious aerobic and anaerobic bacteria 500 GH-14202-05 Brilliant green bile broth A standard methods medium for confirmed and completed tests for coliforms in water 500 GH-14200-14 Buffered peptone water Helps in the recovery of Salmonella from foods after preservation techniques 500 GH-14202-08 Cetrimide agar Use for the selective isolation of Pseudomonas aeruginosa 500 GH-14200-52 This broth is especially suited for environmental sampling where neutralization of the chemical D/E neutralizing Broth 500 GH-14202-11 is important to determining the bactericidal activity Use to isolate E. -
Macconkey Agar Base
MACCONKEY AGAR BASE INTENDED USE Remel MacConkey Agar Base is a solid medium recommended for use in qualitative procedures for ithe cultivation of gram-negative bacilli. SUMMARY AND EXPLANATION In 1900, MacConkey first described a neutral red bile salt medium for cultivation and identification of enteric organisms.1 A detailed description of the selective and differential properties of the medium was published in 1905.2 Over the years, MacConkey’s original formula has been modified; the agar content has been reduced, the concentration of bile salts and neutral red has been adjusted, and sodium chloride has been added.3 MacConkey Agar Base is used with added carbohydrate to differentiate enteric gram-negative bacilli based on fermentation reactions. PRINCIPLE Peptones provide nitrogenous nutrients and amino acids necessary for bacterial growth. Sodium chloride supplies essential electrolytes and maintains osmotic equilibrium. Crystal violet and bile salts are selective agents which inhibit most gram-positive organisms. MacConkey Agar Base is used with added carbohydrate to differentiate enteric gram-negative bacilli based on fermentation reactions. When the carbohydrate is fermented, a local pH drop around the colony causes bile preciptitation in the agar around the colony. Neutral red is an indicator which turns colonies pink when the carbohydrate is fermented. Agar is a solidifying agent. REAGENTS (CLASSICAL FORMULA)* Gelatin Peptone .............................................................. 17.0 g Meat Peptone ..................................................................1.5 -
CTA with Carbohydrates Is a Semi-Solid Medium Suitable for the Determination of Fermentation Reactions of Fastidious Microorganisms
Administrative Offices Phone: 207-873-7711 Fax: 207-873-7022 Customer Service Phone: 1-800-244-8378 P.O. Box 788 Fax: 207-873-7022 Waterville, Maine 04903-0788 RT. 137, China Road Winslow, Maine 04901 TECHNICAL PRODUCT INFORMATION CYSTINE TRYPTIC AGAR [CTA] w/ or w/o CARBOHYDRATES Catalog No: T1400 Control (w/o Carbohydrates) T1410 CTA w/DEXTROSE T1440 CTA w/MALTOSE T1420 CTA w/FRUCTOSE T1445 CTA w/MANNITOL T1430 CTA w/LACTOSE T1450 CTA w/SUCROSE T1435 CTA w/XYLOSE T0340 CTA w/SORBOSE T0350 CTA w/INULIN T0355 CTA w/SORBITOL INTENDED USE: CTA with carbohydrates is a semi-solid medium suitable for the determination of fermentation reactions of fastidious microorganisms. CTA medium without carbohydrates is suitable for maintenance of organisms, and for detection of motility. HISTORY/SUMMARY: CTA medium has been accepted for the determination of carbohydrate utilization for a number of fastidious organisms, particularly Neisseria species and anaerobes. It has also been reported useful in fermentation studies of yeast. As a maintenance medium without carbohydrates, it supports the growth of organisms such as Neisseria, Pasteurella, Streptococci, Brucella, Corynebacteria and others. Motility can be detected in the semisolid medium when inoculated by stab line. PRINCIPLES: The base medium is free of carbohydrates and meat extracts. It contains Cystine and Casein Peptone as nutrients for the growth of fastidious organisms. Phenol red is added as an indicator of fermentation reactions. Carbohydrates are usually incorporated in the medium in 1% final concentrations. If a microorganism is inoculated in the medium containing a carbohydrate, and is capable of fermenting it, the medium indicator will turn from orange red to yellow. -
Superficieibacter Electus Gen. Nov., Sp. Nov., an Extended-Spectrum β
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Digital Commons@Becker Washington University School of Medicine Digital Commons@Becker Open Access Publications 2018 Superficieibacter electus gen. nov., sp. nov., an extended-spectrum β-lactamase possessing member of the enterobacteriaceae family, isolated from Intensive Care Unit surfaces Robert F. Potter Washington University School of Medicine in St. Louis Alaric W. D-Souza Washington University School of Medicine in St. Louis Meghan A. Wallace Washington University School of Medicine in St. Louis Angela Shupe Washington University School of Medicine in St. Louis Sanket Patel Washington University School of Medicine in St. Louis See next page for additional authors Follow this and additional works at: https://digitalcommons.wustl.edu/open_access_pubs Recommended Citation Potter, Robert F.; D-Souza, Alaric W.; Wallace, Meghan A.; Shupe, Angela; Patel, Sanket; Gul, Danish; Kwon, Jennie H.; Beatty, Wandy; Andleeb, Saadia; Burnham, Carey-Ann D.; and Dantas, Gautam, ,"Superficieibacter electus gen. nov., sp. nov., an extended- spectrum β-lactamase possessing member of the enterobacteriaceae family, isolated from Intensive Care Unit surfaces." Frontiers in Microbiology.9,. 1629. (2018). https://digitalcommons.wustl.edu/open_access_pubs/7020 This Open Access Publication is brought to you for free and open access by Digital Commons@Becker. It has been accepted for inclusion in Open Access Publications by an authorized administrator of Digital Commons@Becker. For more information, please contact [email protected]. Authors Robert F. Potter, Alaric W. D-Souza, Meghan A. Wallace, Angela Shupe, Sanket Patel, Danish Gul, Jennie H. Kwon, Wandy Beatty, Saadia Andleeb, Carey-Ann D. -
Bile Esculin Azide Agar (ISO 7899-2:2000) (Dehydrated Culture Media) for Microbiology
PRODUCT CODE: 415523 Bile Esculin Azide Agar (ISO 7899-2:2000) (Dehydrated Culture Media) for microbiology Preparation Suspend 56.6 grams of the medium in one litre of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121ºC for 15 minutes. Overheating can cause darkening of the medium. If tubes are used, allow cooling in a slanted position. The prepared medium should be stored at 8-15°C. The colour is tournasol. The dehydrated medium should be homogeneous, free-flowing and toasted in colour. If there are any physical changes, discard the medium. Caution: This medium is toxic if swallowed, inhaled or comes into contact with skin. Wear gloves and eye/face protection. Uses BILE ESCULIN AZIDE AGAR is a modification of Bile Esculin Agar with the addition of sodium azide as an inhibitor and with the reduction of the bile concentration. The resulting medium is more selective but still provides rapid growth and efficient recovery of enterococci. The ability to hydrolyse esculin in the presence of bile is a characteristic of enterococci. Organisms positive for esculin hydrolysis hydrolyse the glycoside esculin to esculetin and dextrose. The esculetin reacts with the Ferric citrate to form a dark brown or black colony. Ox bile does not inhibit enterococci while other Gram-positive bacteria are inhibited. Sodium azide inhibits Gram negative bacteria. Tryptone, Peptone and Yeast extract supply the nutrients essential for growth. Sodium chloride provides the osmotic balance. Bacteriological agar is the solidifying agent.