Dehydrated Culture Media
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Pocket Guide to Clinical Microbiology
4TH EDITION Pocket Guide to Clinical Microbiology Christopher D. Doern 4TH EDITION POCKET GUIDE TO Clinical Microbiology 4TH EDITION POCKET GUIDE TO Clinical Microbiology Christopher D. Doern, PhD, D(ABMM) Assistant Professor, Pathology Director of Clinical Microbiology Virginia Commonwealth University Health System Medical College of Virginia Campus Washington, DC Copyright © 2018 Amer i can Society for Microbiology. All rights re served. No part of this publi ca tion may be re pro duced or trans mit ted in whole or in part or re used in any form or by any means, elec tronic or me chan i cal, in clud ing pho to copy ing and re cord ing, or by any in for ma tion stor age and re trieval sys tem, with out per mis sion in writ ing from the pub lish er. Disclaimer: To the best of the pub lish er’s knowl edge, this pub li ca tion pro vi des in for ma tion con cern ing the sub ject mat ter cov ered that is ac cu rate as of the date of pub li ca tion. The pub lisher is not pro vid ing le gal, med i cal, or other pro fes sional ser vices. Any ref er ence herein to any spe cific com mer cial prod ucts, pro ce dures, or ser vices by trade name, trade mark, man u fac turer, or oth er wise does not con sti tute or im ply en dorse ment, rec om men da tion, or fa vored sta tus by the Ameri can Society for Microbiology (ASM). -
CTA with Carbohydrates Is a Semi-Solid Medium Suitable for the Determination of Fermentation Reactions of Fastidious Microorganisms
Administrative Offices Phone: 207-873-7711 Fax: 207-873-7022 Customer Service Phone: 1-800-244-8378 P.O. Box 788 Fax: 207-873-7022 Waterville, Maine 04903-0788 RT. 137, China Road Winslow, Maine 04901 TECHNICAL PRODUCT INFORMATION CYSTINE TRYPTIC AGAR [CTA] w/ or w/o CARBOHYDRATES Catalog No: T1400 Control (w/o Carbohydrates) T1410 CTA w/DEXTROSE T1440 CTA w/MALTOSE T1420 CTA w/FRUCTOSE T1445 CTA w/MANNITOL T1430 CTA w/LACTOSE T1450 CTA w/SUCROSE T1435 CTA w/XYLOSE T0340 CTA w/SORBOSE T0350 CTA w/INULIN T0355 CTA w/SORBITOL INTENDED USE: CTA with carbohydrates is a semi-solid medium suitable for the determination of fermentation reactions of fastidious microorganisms. CTA medium without carbohydrates is suitable for maintenance of organisms, and for detection of motility. HISTORY/SUMMARY: CTA medium has been accepted for the determination of carbohydrate utilization for a number of fastidious organisms, particularly Neisseria species and anaerobes. It has also been reported useful in fermentation studies of yeast. As a maintenance medium without carbohydrates, it supports the growth of organisms such as Neisseria, Pasteurella, Streptococci, Brucella, Corynebacteria and others. Motility can be detected in the semisolid medium when inoculated by stab line. PRINCIPLES: The base medium is free of carbohydrates and meat extracts. It contains Cystine and Casein Peptone as nutrients for the growth of fastidious organisms. Phenol red is added as an indicator of fermentation reactions. Carbohydrates are usually incorporated in the medium in 1% final concentrations. If a microorganism is inoculated in the medium containing a carbohydrate, and is capable of fermenting it, the medium indicator will turn from orange red to yellow. -
The Cultivable Autochthonous Microbiota of the Critically Endangered Northern Bald Ibis (Geronticus Eremita)
RESEARCH ARTICLE The cultivable autochthonous microbiota of the critically endangered Northern bald ibis (Geronticus eremita) Joachim Spergser1*, Igor Loncaric1, Alexander Tichy2, Johannes Fritz3, Alexandra Scope4 1 Institute of Microbiology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria, 2 Bioinformatics and Biostatistics Platform, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria, 3 Waldrappteam, Mutters, Austria, 4 Clinical Unit of Internal Medicine Small a1111111111 Animals, Department/Clinic for Companion Animals and Horses, University of Veterinary Medicine, Vienna, a1111111111 Austria a1111111111 a1111111111 * [email protected] a1111111111 Abstract The critically endangered Northern bald ibis (Geronticus eremita) is a migratory bird that OPEN ACCESS became extinct in Europe centuries ago. Since 2014, the Northern bald ibis is subject to an Citation: Spergser J, Loncaric I, Tichy A, Fritz J, intensive rehabilitation and conservation regime aiming to reintroduce the bird in its original Scope A (2018) The cultivable autochthonous distribution range in Central Europe and concurrently to maintain bird health and increase microbiota of the critically endangered Northern bald ibis (Geronticus eremita). PLoS ONE 13(4): population size. Hitherto, virtually nothing is known about the microbial communities associ- e0195255. https://doi.org/10.1371/journal. ated with the ibis species; an information pivotal for the veterinary management of these pone.0195255 birds. Hence, the present study was conducted to provide a baseline description of the culti- Editor: Michael Lierz, Justus-Liebeig University vable microbiota residing in the Northern bald ibis. Samples derived from the choana, tra- Giessen, GERMANY chea, crop and cloaca were examined employing a culturomic approach in order to identify Received: June 30, 2017 microbes at each sampling site and to compare their frequency among age classes, sea- Accepted: March 19, 2018 sonal appearances and rearing types. -
Prepared Culture Media
PREPARED CULTURE MEDIA 121517SS PREPARED CULTURE MEDIA Made in the USA AnaeroGRO™ DuoPak A 02 Bovine Blood Agar, 5%, with Esculin 13 AnaeroGRO™ DuoPak B 02 Bovine Blood Agar, 5%, with Esculin/ AnaeroGRO™ BBE Agar 03 MacConkey Biplate 13 AnaeroGRO™ BBE/PEA 03 Bovine Selective Strep Agar 13 AnaeroGRO™ Brucella Agar 03 Brucella Agar with 5% Sheep Blood, Hemin, AnaeroGRO™ Campylobacter and Vitamin K 13 Selective Agar 03 Brucella Broth with 15% Glycerol 13 AnaeroGRO™ CCFA 03 Brucella with H and K/LKV Biplate 14 AnaeroGRO™ Egg Yolk Agar, Modified 03 Buffered Peptone Water 14 AnaeroGRO™ LKV Agar 03 Buffered Peptone Water with 1% AnaeroGRO™ PEA 03 Tween® 20 14 AnaeroGRO™ MultiPak A 04 Buffered NaCl Peptone EP, USP 14 AnaeroGRO™ MultiPak B 04 Butterfield’s Phosphate Buffer 14 AnaeroGRO™ Chopped Meat Broth 05 Campy Cefex Agar, Modified 14 AnaeroGRO™ Chopped Meat Campy CVA Agar 14 Carbohydrate Broth 05 Campy FDA Agar 14 AnaeroGRO™ Chopped Meat Campy, Blood Free, Karmali Agar 14 Glucose Broth 05 Cetrimide Select Agar, USP 14 AnaeroGRO™ Thioglycollate with Hemin and CET/MAC/VJ Triplate 14 Vitamin K (H and K), without Indicator 05 CGB Agar for Cryptococcus 14 Anaerobic PEA 08 Chocolate Agar 15 Baird-Parker Agar 08 Chocolate/Martin Lewis with Barney Miller Medium 08 Lincomycin Biplate 15 BBE Agar 08 CompactDry™ SL 16 BBE Agar/PEA Agar 08 CompactDry™ LS 16 BBE/LKV Biplate 09 CompactDry™ TC 17 BCSA 09 CompactDry™ EC 17 BCYE Agar 09 CompactDry™ YMR 17 BCYE Selective Agar with CAV 09 CompactDry™ ETB 17 BCYE Selective Agar with CCVC 09 CompactDry™ YM 17 BCYE -
APPENDIX a Media and Reagents
APPENDIX A Media and Reagents Pauline K. w. Yu, M.S. The use of appropriate and dependable media is integral to the isolation and identification of microorganisms. Unfortunately, comparative data docu menting the relative efficacy or value of media designed for similar purposes are often lacking. Moreover, one cannot presume identity in composition of a given generic product which is manufactured by several companies because each may supplement the generic products with components, often of a proprietary nature and not specified in the product's labeling. Finally, the actual production of similar products may vary among manufacturers to a sufficient extent to affect their performance. For all of these reasons, therefore, product selection for the laboratory should not be strictly based on cost considerations and should certainly not be based on promotional materials. Evaluations that have been published in the scientific literature should be consulted when available. Alternatively, the prospective buyer should consult a recognized authority in the field. It is seldom necessary for the laboratory to prepare media using basic components since these are usually available combined in dehydrated form from commercial sources; however, knowledge of a medium's basic compo nents is helpful in understanding how the medium works and what might be wrong when it does not work. Hence, the components have been listed for each medium included in this chapter. All dehydrated media must be prepared exactly according to the manu facturers' directions. Any deviation from these directions may adversely affect or significantly alter a medium's performance. Containers of media should be dated on receipt and when opened, and the media should never be used beyond expiration dates specified by the manufacturers or recom mended by quality control programs. -
Criterionâ—¢ Cystine Tryptic Agar
CRITERION™ CYSTINE TRYPTIC AGAR (CTA) Cat. no. C5510 CRITERION™ Cystine Tryptic Agar (CTA) 59gm Cat. no. C5511 CRITERION™ Cystine Tryptic Agar (CTA) 500gm Cat. no. C5512 CRITERION™ Cystine Tryptic Agar (CTA) 2kg Cat. no. C5513 CRITERION™ Cystine Tryptic Agar (CTA) 10kg Cat. no. C5514 CRITERION™ Cystine Tryptic Agar (CTA) 50kg INTENDED USE Hardy Diagnostics CRITERION™ Cystine Tryptic Agar (CTA) is recommended for the determination of carbohydrate fermentation by fastidious microorganisms, such as Neisseria spp. It is also used for the detection of bacterial motility and the base can serve as a holding medium for the maintenance of fastidious microorganisms. This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements. SUMMARY In general, Cystine Tryptic Agar (CTA) provides a nutritious basal medium composed of casein peptones, cystine, inorganic salts, phenol red, and agar. The inorganic salts serve as a source of essential ions. Phenol red is the pH color indicator. CRITERION™ Cystine Tryptic Agar (CTA) supplemented with a 1% concentration of a specific carbohydrate is used to detect fermentation reactions. The 1% concentration is recommended to decrease the possibility of reversal reactions. Reversion occurs when the carbohydrate is depleted, thereby resulting in the masking of acid by alkaline by- products from peptone degradation. The acid produced by carbohydrate consumption causes a decrease in pH resulting in a color shift in the medium from red-pink to yellow. The addition of agar to the medium allows for the detection of motility along the stab line of inoculation. -
BD Industry Catalog
PRODUCT CATALOG INDUSTRIAL MICROBIOLOGY BD Diagnostics Diagnostic Systems Table of Contents Table of Contents 1. Dehydrated Culture Media and Ingredients 5. Stains & Reagents 1.1 Dehydrated Culture Media and Ingredients .................................................................3 5.1 Gram Stains (Kits) ......................................................................................................75 1.1.1 Dehydrated Culture Media ......................................................................................... 3 5.2 Stains and Indicators ..................................................................................................75 5 1.1.2 Additives ...................................................................................................................31 5.3. Reagents and Enzymes ..............................................................................................75 1.2 Media and Ingredients ...............................................................................................34 1 6. Identification and Quality Control Products 1.2.1 Enrichments and Enzymes .........................................................................................34 6.1 BBL™ Crystal™ Identification Systems ..........................................................................79 1.2.2 Meat Peptones and Media ........................................................................................35 6.2 BBL™ Dryslide™ ..........................................................................................................80 -
Prepared Culture Media
PREPARED CULTURE MEDIA 030220SG PREPARED CULTURE MEDIA Made in the USA AnaeroGRO™ DuoPak A 02 Bovine Blood Agar, 5%, with Esculin 13 AnaeroGRO™ DuoPak B 02 Bovine Blood Agar, 5%, with Esculin/ AnaeroGRO™ BBE Agar 03 MacConkey Biplate 13 AnaeroGRO™ BBE/PEA 03 Bovine Selective Strep Agar 13 AnaeroGRO™ Brucella Agar 03 Brucella Agar with 5% Sheep Blood, Hemin, AnaeroGRO™ Campylobacter and Vitamin K 13 Selective Agar 03 Brucella Broth with 15% Glycerol 13 AnaeroGRO™ CCFA 03 Brucella with H and K/LKV Biplate 14 AnaeroGRO™ Egg Yolk Agar, Modifi ed 03 Buffered Peptone Water 14 AnaeroGRO™ LKV Agar 03 Buffered Peptone Water with 1% AnaeroGRO™ PEA 03 Tween® 20 14 AnaeroGRO™ MultiPak A 04 Buffered NaCl Peptone EP, USP 14 AnaeroGRO™ MultiPak B 04 Butterfi eld’s Phosphate Buffer 14 AnaeroGRO™ Chopped Meat Broth 05 Campy Cefex Agar, Modifi ed 14 AnaeroGRO™ Chopped Meat Campy CVA Agar 14 Carbohydrate Broth 05 Campy FDA Agar 14 AnaeroGRO™ Chopped Meat Campy, Blood Free, Karmali Agar 14 Glucose Broth 05 Cetrimide Select Agar, USP 14 AnaeroGRO™ Thioglycollate with Hemin and CET/MAC/VJ Triplate 14 Vitamin K (H and K), without Indicator 05 CGB Agar for Cryptococcus 14 Anaerobic PEA 08 Chocolate Agar 15 Baird-Parker Agar 08 Chocolate/Martin Lewis with Barney Miller Medium 08 Lincomycin Biplate 15 BBE Agar 08 CompactDry™ SL 16 BBE Agar/PEA Agar 08 CompactDry™ LS 16 BBE/LKV Biplate 09 CompactDry™ TC 17 BCSA 09 CompactDry™ EC 17 BCYE Agar 09 CompactDry™ YMR 17 BCYE Selective Agar with CAV 09 CompactDry™ ETB 17 BCYE Selective Agar with CCVC 09 CompactDry™ YM 17 -
CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (KV)
Difco & BBL Manual Manual of Microbiological Culture Media Second Edition Editors Mary Jo Zimbro, B.S., MT (ASCP) David A. Power, Ph.D. Sharon M. Miller, B.S., MT (ASCP) George E. Wilson, MBA, B.S., MT (ASCP) Julie A. Johnson, B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks, MD 21152 Difco Manual Preface.ind 1 3/16/09 3:02:34 PM Table of Contents Contents Preface ...............................................................................................................................................................v About This Manual ...........................................................................................................................................vii History of BD Diagnostics .................................................................................................................................ix Section I: Monographs .......................................................................................................................................1 History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media ..................................................................................................................5 Culture Media Ingredients – Agars ...................................................................................................................6 -
LIOFILCHEM S.R.L. Via Scozia Zona Ind.Le - 64026 Roseto D.A
TECHNICAL SHEET TS 34071 Rev. 0 of 04.06.2008 Pag. 1 of 2 CYSTINE TRYPTIC AGAR (CTA) Semi-solid basal medium used with added carbohydrates in differentiating microorganisms on fermentation reactions and motility. TYPICAL FORMULA (g/L) Tryptone 20.0 L- Cystine 0.5 Sodium Sulphite 0.5 Sodium Chloride 5.0 Phenol Red 0.017 Agar 2.5 Final pH 7.3 ± 0.2 DESCRIPTION CYSTINE TRYPTIC AGAR (CTA) is a semi-solid basal medium used with added carbohydrates in differentiating microorganisms on fermentation reactions and motility. PRINCIPLE Tryptone provides nitrogen and other nutrient to support microbial growth. L- Cystine and Sodium Sulphite are added to stimulate growth. Sodium Chloride maintains the osmotic balance of the medium. Phenol Red is the pH indicator. Agar is added to favour anaerobic growth and for the determination of motility. PREPARATION Melt the content of one tube in a boiling water-bath at 100°C (loosing the caps partially unscrewed) until completely dissolved. Cool down to 45-50°C and aseptically add a sterile carbohydrate solution so to obtain a 5-10% concentration. Mix well avoiding the formation of bubbles and aseptically distribute into 5-7 mL tubes. Allow the medium to solidify. TECHNIQUE Touch the colony to test with an inoculation needle and stab the medium. Incubate at 36 ± 1 °C for at least 18-48 hours. INTERPRETATION OF RESULTS Fermentation of the test carbohydrate is observed when acid is formed and the medium turns from red to yellow. Motility of an organism is evident as a haze of growth extending into the agar from the stab line. -
A Non-Canonical Mismatch Repair Pathway in Prokaryotes
ARTICLE Received 29 Jun 2016 | Accepted 12 Dec 2016 | Published 27 Jan 2017 DOI: 10.1038/ncomms14246 OPEN A non-canonical mismatch repair pathway in prokaryotes A. Castan˜eda-Garcı´a1,2, A.I. Prieto1, J. Rodrı´guez-Beltra´n1, N. Alonso3, D. Cantillon4, C. Costas1,L.Pe´rez-Lago5, E.D. Zegeye6, M. Herranz5, P. Plocin´ski2,w, T. Tonjum6, D. Garcı´a de Viedma5, M. Paget7, S.J. Waddell4, A.M. Rojas8, A.J. Doherty2 & J. Bla´zquez1,3,9 Mismatch repair (MMR) is a near ubiquitous pathway, essential for the maintenance of genome stability. Members of the MutS and MutL protein families perform key steps in mismatch correction. Despite the major importance of this repair pathway, MutS–MutL are absent in almost all Actinobacteria and many Archaea. However, these organisms exhibit rates and spectra of spontaneous mutations similar to MMR-bearing species, suggesting the existence of an alternative to the canonical MutS–MutL-based MMR. Here we report that Mycobacterium smegmatis NucS/EndoMS, a putative endonuclease with no structural homology to known MMR factors, is required for mutation avoidance and anti- recombination, hallmarks of the canonical MMR. Furthermore, phenotypic analysis of natu- rally occurring polymorphic NucS in a M. smegmatis surrogate model, suggests the existence of M. tuberculosis mutator strains. The phylogenetic analysis of NucS indicates a complex evolutionary process leading to a disperse distribution pattern in prokaryotes. Together, these findings indicate that distinct pathways for MMR have evolved at least twice in nature. 1 Stress and Bacterial Evolution Group, Instituto de Biomedicina de Sevilla. Avda. Manuel Siurot S/N, 41013-Sevilla, Spain. -
Dehydrated Culture Media Contents
Dehydrated Culture Media Contents CRITERION™ Products 1 Supplements and Antibiotics 11 Blood Products 12 Petri Plates and Tubes 13 DEHYDRATED CULTURE MEDIA Choose from 250 standard formulas or request custom blending to your specifications. The innovative packaging designs and overall reliability make CRITERION™ the logical choice for culture media in your laboratory. Dehydrated Culture Media FEATURES & BENEFITS GRAY WIDE MOUTH ✓ Allows for easy access to use a scoop when measuring the powder. JAR OPENING ✓ Prevents inhalation hazards and reduces hazardous dust formations. Opaque gray jar ✓ No more shaking the bottle to dispense the media. diminishes light penetration, prolonging INDUCTION Peel-off induction seal prevents moisture from clumping performance SEAL the media, keeping it fresh and dry. and shelf life. REUSABLE A built-in cushion seal inside the lid SEAL prevents moisture from entering the previously opened container. 1 5 2 LITER POUCH 500GM JAR 2 KG BUCKET • Mylar® zip pouch bag • 500gm bottle • 2kg high density • Pre-measured to make 2 • Stackable containers polyethlene buckets liters of culture media with handle, and • No need for weighing locking screw top lid 10 KG BUCKET 50 KG BARREL • 10kg high density polyethlene • 50kg high density polyethlene buckets with handle, and locking drum screw top lid 2 CUSTOM FORMULATIONS Need a specialized formulation? Hardy Diagnostics is ready to assist you with your specific needs. Our Quality Management System is ISO 13485 certified and licensed by the FDA as an In vitro Medical Device Manufacturer, ensuring the highest standards of quality for our customers. Call: 800.266.2222 Today! 3 ® PRODUCT DESCRIPTION Mylar 500gm 2kg 10kg Zip-Bag Bottle Bucket Bucket A-1 Medium C7570 C7571 C7572 C7573 Agar, Bacteriological Grade C5000 C5001 C5002 C5003 Agar, Pharmaceutical Grade C7430 C7431 C7432 C7433 Ampicillin Dextrin Agar Base (14) C7580 C7581 C7582 C7583 Antibiotic Medium No.