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Chocolate Agar Plate MP103 Intended Use for Isolation of Neisseria Gonorrhoeae from Chronic and Acute Gonococcal Infections
Chocolate Agar Plate MP103 Intended use For isolation of Neisseria gonorrhoeae from chronic and acute gonococcal infections. Composition** Ingredients Gms / Litre Proteose peptone 20.000 Dextrose 0.500 Sodium chloride 5.000 Disodium phosphate 5.000 Agar 15.000 After sterilization Sterile Lysed blood (at 80°C) 50.000 Vitamino Growth Supplement (FD025) 2 vials Final pH ( at 25°C) 7.3±0.2 **Formula adjusted, standardized to suit performance parameters Directions Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate. Principle And Interpretation Neisseria gonorrhoeae is a gram-negative bacteria and the causative agent of gonorrhea, however it is also occasionally found in the throat. The cultivation medium for gonococci should ideally be a rich nutrients base with blood, either partially lysed or completely lysed. The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoeae. Chocolate Agar Base, with the addition of supplements, gives excellent growth of the gonococcus without overgrowth by contaminating organisms. G.C. Agar (M434) can also be used in place of Chocolate Agar Base, which gives slightly better results than Chocolate Agar (4). The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoea. Interest in the cultural procedure for the diagnosis of gonococcal infection was stimulated by Ruys and Jens (9), Mcleod and co-workers (8), Thompson (7), Leahy and Carpenter (1), Carpenter, Leahy and Wilson (2) and Carpenter (10), who clearly demonstrated the superiority of this method over the microscopic technique. -
Microbiology Media - Ready to Use, Prepared Plates
Microbiology Media - Ready to use, prepared plates Ready poured plates, general purpose media Ready poured plate, general anaerobe agar 65 65 Columbia blood agar with neomycin. Chromogenic UTI medium Catalogue No Plate diameter, mm Pack qty Price Catalogue No Pack qty Price PO794A 90 10 12.55 PO219A 10 7.54 CLED medium Catalogue No Plate diameter, mm Pack qty Price Ready poured plate, Lactobacilli PO120A 90 10 5.75 64 CLED square plate Catalogue No Plate dimensions, mm Pack qty Price MRS Agar. OXPO0299L 120 x 120 10 16.92 CLED medium with andrades Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO231A 90 10 7.30 PO121A 90 10 5.75 Columbia agar base Ready poured plates, Legionnella media Catalogue No Plate diameter, mm Pack qty Price 65 OXPO0537A 90 10 5.45 MacConkey agar with salt Legionella growth medium, BCYE Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO149A 90 10 5.95 PO5072A 90 10 14.72 MacConkey agar without salt Legionella selective medium, BMPA Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO148A 90 10 5.58 PO0324A 90 10 20.31 MacConkey agar No. 3 Catalogue No Plate diameter, mm Pack qty Price PO495A 90 10 5.90 Malt extract agar Catalogue No Plate diameter, mm Pack qty Price PO182A 90 10 6.38 MRSA agar Catalogue No Plate diameter, mm Pack qty Price OXPO1162A 90 10 15.49 Nutrient agar Catalogue No Plate diameter, mm Pack qty Price PO155A 90 10 5.75 Plate count agar Catalogue No Plate diameter, mm Pack qty Price PO158A 90 10 6.36 R2A agar Catalogue No Plate diameter, mm Pack qty Price PO659A 90 10 6.50 Sabouraud dextrose agar Catalogue No Plate diameter, mm Pack qty Price OXPO0160A 90 10 5.54 Sorbitol MacConkey agar Catalogue No Plate diameter, mm Pack qty Price PO232A 90 10 5.84 Tryptone soya agar Catalogue No Plate diameter, mm Pack qty Price PO163A 90 10 5.49 OXPO0193-C 55 10 10.91 Yeast extract agar Catalogue No Plate diameter, mm Pack qty Price PO441A 90 10 5.08 55. -
Estudio Molecular De Poblaciones De Pseudomonas Ambientales
Universitat de les Illes Balears ESTUDIO MOLECULAR DE POBLACIONES DE PSEUDOMONAS AMBIENTALES T E S I S D O C T O R A L DAVID SÁNCHEZ BERMÚDEZ DIRECTORA: ELENA GARCÍA-VALDÉS PUKKITS Departamento de Biología Universitat de les Illes Balears Palma de Mallorca, Septiembre 2013 Universitat de les Illes Balears ESTUDIO MOLECULAR DE POBLACIONES DE PSEUDOMONAS AMBIENTALES Tesis Doctoral presentada por David Sánchez Bermúdez para optar al título de Doctor en el programa Microbiología Ambiental y Biotecnología, de la Universitat de les Illes Balears, bajo la dirección de la Dra. Elena García-Valdés Pukkits. Vo Bo Director de la Tesis El doctorando DRA. ELENA GARCÍA-VALDÉS PUKKITS DAVID SÁNCHEZ BERMÚDEZ Catedrática de Universidad Universitat de les Illes Balears PALMA DE MALLORCA, SEPTIEMBRE 2013 III IV Index Agradecimientos .................................................................................................... IX Resumen ................................................................................................................ 1 Abstract ................................................................................................................... 3 Introduction ............................................................................................................ 5 I.1. The genus Pseudomonas ............................................................................................ 7 I.1.1. Definition ................................................................................................................ 7 I.1.2. -
Dehydrated Culture Media
Dehydrated Culture Media Manufactured by Dehydrated Culture Media Table of Contents 4 CRITERION™ Products 12 Supplements and Antibiotics 13 CRITERION™ Agarose for Gel Electrophoresis Dehydrated Culture Media ™ TM Hardy Diagnostics’ dehydrated culture media, CRITERION , is formulated to meet or exceed the highest quality standards. DEHYDRATED CULTURE MEDIA Choose from 250 standard formulas or request custom blending to your specifications. The innovative packaging designs and overall reliability makeCRITERION ™ the logical choice for culture media in your laboratory. FEATURES & BENEFITS Hand Grip Convenient hand-grip design features finger indentations to allow for easy and safe handling of the bottle. Induction Seal Gray Jar Pull-off induction seal prevents moisture from clumping the media, keeping it fresh and dry. Opaque gray jar diminishes Wide Mouth Opening light penetration, • Allows for easy access to use a scoop when prolonging superior measuring the powder. performance and • Prevents inhalation hazards and reduces shelf life. hazardous dust formations. • No more shaking the bottle to dispense the media. Desiccant Pack A silica gel pack is included in each bottle to prevent clumping. Reusable Seal A built-in cushion seal inside the lid prevents moisture from entering the previously opened container. 1 UNPARALLELED PERFORMANCE Every formulation and lot is thoroughly tested for optimal growth characteristics. WIDE MOUTH OPENING Scooping media from the wide mouth bottle, instead of pouring and shaking, reduces dangerous dust formation CONVENIENT SIZES Packaged in four standard sizes to fit your needs: • 2 liter Mylar® bag (pre-measured to make 2 liters of culture media) • 500gm bottle • 2kg buckets with locking screw top lid • 10kg buckets with locking screw top lid STACKABLE Bottles and buckets have a nesting design and are stackable for efficient and economical storage. -
Dehydrated Culture Media Description Packaging Ref
Product Catalogue 2016 © Liofilchem® s.r.l. Clinical and Industrial Microbiology Est. 1983 Dehydrated Culture Media Description Packaging Ref. A1 Medium APHA 500 g 610105 Basal liquid medium for fecal coliforms detection in water and food. 100 g 620105 TRITON X 100 supplement 5x5 mL 80046 Acetamide Agar 500 g 610312 Medium for differentiation of nonfermentative, Gram-negative bacteria, especially Pseudomonas aeruginosa, on the basis of acetamide utilization. Acetamide Broth 500 g 610313 Broth for the differentiation of nonfermentative, Gram-negative bacteria, especially Pseudomonas aeruginosa, on the basis of acetamide utilization. Aeromonas Agar Base 500 g 610048 Basal medium for selective isolation of Aeromonas spp. 100 g 620048 Ampicillin supplement 10 vials 81001 Alkaline Peptone Water APHA 500 g 610098 Liquid enrichment medium for Vibrio spp. isolation. 100 g 620098 Amies Transport Medium (with charcoal) 500 g 610152 Semi-solid medium for transport of clinical, environmental specimens and of 100 g 620152 microorganisms. 5 kg 6101525 Amies Transport Medium (w/o charcoal) 500 g 610191 Semi-solid medium for transport of clinical, environmental specimens and of 100 g 620191 microorganisms. 5 kg 6101915 Anaerobic Agar (Brewer) 500 g 610320 Medium for cultivating anaerobic microorganisms. Andrade Lactose Peptone Water 500 g 610118 Liquid medium for coliforms detection with andrade's indicator. 100 g 620118 Andrade Peptone Water 500 g 610119 Liquid enrichment medium with andrade's indicator. 100 g 620119 Antibiotic Agar No.1 E.P. 500 g 610314 Surface medium for the antibiotic assay by Agar-diffusion method. Antibiotic Broth No.3 U.S.P. 500 g 610316 Broth for turbidimetric assay of antibiotics. -
Bile Esculin Agar
BILE ESCULIN AGAR INTENDED USE Remel Bile Esculin Agar is a solid medium recommended for use in qualitative procedures for the presumptive identification of group D streptococci and enterococci. SUMMARY AND EXPLANATION Rochaix first demonstrated the value of esculin hydrolysis for identification of enterococci.1 Meyer et al. found that 61 of 62 strains of enterococci hydrolyzed esculin in a medium containing bile.2 Swan determined that positive results obtained on esculin agar containing 40% bile correlated well with serologically confirmed group D streptococci.3 Using Swan’s formula, Facklam and Moody tested over 700 strains of streptococci and enterococci representing all known serological groups and found all strains to be bile-resistant and esculin-positive. PRINCIPLE Group D streptococci and enterococci hydrolyze esculin in the presence of bile to form esculetin and dextrose. Esculetin reacts with ferric ions supplied by ferric ammonium citrate to form brown-black colonies on Bile Esculin Agar. Oxgall in a concentration of 4% (equivalent to 40% bile) inhibits most strains of streptococci and enterococci other than group D. REAGENTS (CLASSICAL FORMULA)* Oxgall (40% Bile) ............................................................ 40.0 g Esculin...............................................................................1.0 g Gelatin Peptone ................................................................ 5.0 g Ferric Ammonium Citrate...................................................0.5 g Beef Extract....................................................................... 3.0 g Agar.................................................................................15.0 g Demineralized Water...................................................1000.0 ml pH 6.8 ± 0.2 @ 25°C *Adjusted as required to meet performance standards. PRECAUTIONS This product is For Laboratory Use only. It is not intended for use in the diagnosis of disease or other conditions. PREPARATION OF DEHYDRATED CULTURE MEDIUM 1. Suspend 64 g of medium in 1000 ml of demineralized water. -
Evaluating Historical Paradigms of Sterility in Perinatal Microbiology and Ramifications Orf Pregnancy Outcomes
Wayne State University Wayne State University Dissertations January 2020 Evaluating Historical Paradigms Of Sterility In Perinatal Microbiology And Ramifications orF Pregnancy Outcomes Jonathan Greenberg Wayne State University Follow this and additional works at: https://digitalcommons.wayne.edu/oa_dissertations Part of the Microbiology Commons, and the Obstetrics and Gynecology Commons Recommended Citation Greenberg, Jonathan, "Evaluating Historical Paradigms Of Sterility In Perinatal Microbiology And Ramifications orF Pregnancy Outcomes" (2020). Wayne State University Dissertations. 2468. https://digitalcommons.wayne.edu/oa_dissertations/2468 This Open Access Dissertation is brought to you for free and open access by DigitalCommons@WayneState. It has been accepted for inclusion in Wayne State University Dissertations by an authorized administrator of DigitalCommons@WayneState. EVALUATING HISTORICAL PARADIGMS OF STERILITY IN PERINATAL MICROBIOLOGY AND RAMIFICATIONS FOR PREGNANCY OUTCOMES by JONATHAN MURRAY GREENBERG DISSERTATION Submitted to the Graduate School of Wayne State University, Detroit, Michigan in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY 2020 MAJOR: BIOCHEMISTRY, MICROBIOLOGY, & IMMUNOLOGY Approved by: Advisor Date DEDICATION This dissertation is dedicated to my parents and sister, who have all been immeasurably supportive over my graduate career and more importantly my entire life. I could not have done this without you. ii ACKNOWLEDGMENTS There is no way for me to express fully my sincere gratitude to all the individuals who have had an impact on my life and throughout my time here at Wayne State. None of it would have been possible without Dr. Kevin Theis. Rotating in his lab was one of the best decisions of my life and quite literally got me to where I am. -
Francisella Tularensis 6/06 Tularemia Is a Commonly Acquired Laboratory Colony Morphology Infection; All Work on Suspect F
Francisella tularensis 6/06 Tularemia is a commonly acquired laboratory Colony Morphology infection; all work on suspect F. tularensis cultures .Aerobic, fastidious, requires cysteine for growth should be performed at minimum under BSL2 .Grows poorly on Blood Agar (BA) conditions with BSL3 practices. .Chocolate Agar (CA): tiny, grey-white, opaque A colonies, 1-2 mm ≥48hr B .Cysteine Heart Agar (CHA): greenish-blue colonies, 2-4 mm ≥48h .Colonies are butyrous and smooth Gram Stain .Tiny, 0.2–0.7 μm pleomorphic, poorly stained gram-negative coccobacilli .Mostly single cells Growth on BA (A) 48 h, (B) 72 h Biochemical/Test Reactions .Oxidase: Negative A B .Catalase: Weak positive .Urease: Negative Additional Information .Can be misidentified as: Haemophilus influenzae, Actinobacillus spp. by automated ID systems .Infective Dose: 10 colony forming units Biosafety Level 3 agent (once Francisella tularensis is . Growth on CA (A) 48 h, (B) 72 h suspected, work should only be done in a certified Class II Biosafety Cabinet) .Transmission: Inhalation, insect bite, contact with tissues or bodily fluids of infected animals .Contagious: No Acceptable Specimen Types .Tissue biopsy .Whole blood: 5-10 ml blood in EDTA, and/or Inoculated blood culture bottle Swab of lesion in transport media . Gram stain Sentinel Laboratory Rule-Out of Francisella tularensis Oxidase Little to no growth on BA >48 h Small, grey-white opaque colonies on CA after ≥48 h at 35/37ºC Positive Weak Negative Positive Catalase Tiny, pleomorphic, faintly stained, gram-negative coccobacilli (red, round, and random) Perform all additional work in a certified Class II Positive Biosafety Cabinet Weak Negative Positive *Oxidase: Negative Urease *Catalase: Weak positive *Urease: Negative *Oxidase, Catalase, and Urease: Appearances of test results are not agent-specific. -
Pocket Guide to Clinical Microbiology
4TH EDITION Pocket Guide to Clinical Microbiology Christopher D. Doern 4TH EDITION POCKET GUIDE TO Clinical Microbiology 4TH EDITION POCKET GUIDE TO Clinical Microbiology Christopher D. Doern, PhD, D(ABMM) Assistant Professor, Pathology Director of Clinical Microbiology Virginia Commonwealth University Health System Medical College of Virginia Campus Washington, DC Copyright © 2018 Amer i can Society for Microbiology. All rights re served. No part of this publi ca tion may be re pro duced or trans mit ted in whole or in part or re used in any form or by any means, elec tronic or me chan i cal, in clud ing pho to copy ing and re cord ing, or by any in for ma tion stor age and re trieval sys tem, with out per mis sion in writ ing from the pub lish er. Disclaimer: To the best of the pub lish er’s knowl edge, this pub li ca tion pro vi des in for ma tion con cern ing the sub ject mat ter cov ered that is ac cu rate as of the date of pub li ca tion. The pub lisher is not pro vid ing le gal, med i cal, or other pro fes sional ser vices. Any ref er ence herein to any spe cific com mer cial prod ucts, pro ce dures, or ser vices by trade name, trade mark, man u fac turer, or oth er wise does not con sti tute or im ply en dorse ment, rec om men da tion, or fa vored sta tus by the Ameri can Society for Microbiology (ASM). -
Detection of Staphylococcus Aureus and Other Coagulase Positive Staphylococci in Bovine Raw Milk in Khartoum State by Ikhtyar Ah
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by KhartoumSpace Detection of Staphylococcus aureus and other Coagulase Positive Staphylococci in Bovine Raw Milk in Khartoum State By Ikhtyar Ahmed Hassan Ali B.C.Sc (2003) Supervisor Prof. Mohammed Taha Shigiddi Department of Microbiology Faculty of Veterinary medicine A dissertation submitted to University of Khartoum in partial fulfillment for the requirement of the Degree of Master of Science in Microbiology Department of Microbiology Faculty of veterinary Medicine University of Khartoum 2010 Dedication to my father, mother, brothers and sisters with love I Table of Contents Subject Page Dedication………………………………………………………. I Table of Contents………………………………………………. II List of Figures…………………………………………………… VII List of Table…………………………………………………….. VIII Acknowledgments………………………………………………. IX Abstract…………………………………………………………. X Abstract (Arabic)……………………………………………… XI Introduction…………………………………………………… 1 Chapter One: Literature Review…………………………….. 3 1.1. Health Hazards of Raw Milk…………………………………… 4 1.2. Pathogenic bacteria in milk........................................................ 5 1.3. Microbial quality of raw milk.................................................... 6 1.4. Staphylococci........................................................................... 7 1.4.1. Coagulase positive staphylococci (CPS)……………………… 8 1.4.2. Coagulase negative staphylococci (CNS)……………………… 10 1.5. Staphylococcus aureus………………………………………… 10 1.5.1. Virulence characteristics of S. -
Get Consistent and Reliable Results
Need help selecting? M Microbiology Free Tech Support 800-323-4340 Cole-Parmer® Dehydrated Culture Media Get consistent and reliable results – Use when ready or dehydrated culture extends shelf life – Individually numbered with certificate of compliance expiration date, and shelf-life guarantee – Quick dissolve for faster processing – For laboratory use only Media, Dehydrated Media, Media Description Size (g) Catalog number Price Agar, bacteriological This clear gel agar is high grade and manufactured using the ice method 500 GH-14200-10 Azide dextrose broth Use for detection of fecal Streptococci in water and sewage 500 GH-14202-00 Baird parker agar Use for the selective isolation of coagulase positive Staphylococci in food 500 GH-14202-01 Blood agar base with low pH Use to cultivate a wide variety of bacteria 500 GH-14202-02 This is used as a differential medium in the isolation and presumptive identification of Bile Esculin agar 500 GH-14202-03 enterococci/group D streptococci Brain heart infusion agar Use to cultivate a wide spectrum of bacteria, yeasts, and molds 500 GH-14200-12 Brain heart infusion broth Use to cultivate fastidious aerobic and anaerobic bacteria 500 GH-14202-05 Brilliant green bile broth A standard methods medium for confirmed and completed tests for coliforms in water 500 GH-14200-14 Buffered peptone water Helps in the recovery of Salmonella from foods after preservation techniques 500 GH-14202-08 Cetrimide agar Use for the selective isolation of Pseudomonas aeruginosa 500 GH-14200-52 This broth is especially suited for environmental sampling where neutralization of the chemical D/E neutralizing Broth 500 GH-14202-11 is important to determining the bactericidal activity Use to isolate E. -
(12) Patent Application Publication (10) Pub. No.: US 2011/0206654 A1 Hodin Et Al
US 2011 0206654A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2011/0206654 A1 Hodin et al. (43) Pub. Date: Aug. 25, 2011 (54) METHODS OF MODULATING Related U.S. Application Data GASTRONTESTINAL TRACTFLORA LEVELS WITH ALKALINE PHOSPHATASE (60) Provisional application No. 61/093,129, filed on Aug. 29, 2008. (75) Inventors: Richard A. Hodin, Newton, MA (US); Madhu S. Malo, Burlington, Publication Classification MA (US) (51) Int. Cl. (73) Assignee: THE GENERAL, HOSPITAL A638/46 (2006.01) CORPORATION, Boston, MA CI2N 9/16 (2006.01) (US) A6IPI/00 (2006.01) (21) Appl. No.: 13/060,863 (52) U.S. Cl. ........................................ 424/94.6; 435/196 (22) PCT Fled: Aug. 27, 2009 (57) ABSTRACT (86) PCT NO.: PCT/USO9/55216 This invention relates generally to a therapeutic use of alka S371 (c)(1), line phosphatase to modulate gastrointestinal tract flora levels (2), (4) Date: May 5, 2011 in Subject. C 7 e 4 ---. W. Mouse Stoo B Mac Brucella MacConkey Agar Plates Media Aerobic Condition) B D WT AP-KO n . 1 ^: O Š AP-KO Mouse Stool Brucella MacConkey Agar Plates Media (Anaerobic Condition) Patent Application Publication Aug. 25, 2011 Sheet 1 of 7 US 2011/0206654 A1 F.G. 1 W &ws s IAP-KOS ls S 4 S.Š 3 WT Mouse Stoo LB BH Mac Brucella MacConkey Agar Plates Media (Aerobic Condition) D 12 SS 9 : AP-KO Mouse Stool Brucella MacConkey Agar Plates Media (Anaerobic Condition) Patent Application Publication Aug. 25, 2011 Sheet 2 of 7 US 2011/0206654 A1 FIG 2 $2$3$?2 8?duesdødsweeg38),jojaquumN A.