DOCSLIB.ORG

Prepared Culture Media

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Prepared Culture Media PREPARED CULTURE MEDIA 030220SG PREPARED CULTURE MEDIA Made in the USA AnaeroGRO™ DuoPak A 02 Bovine Blood Agar, 5%, with Esculin 13 AnaeroGRO™ DuoPak B 02 Bovine Blood Agar, 5%, with Esculin/ AnaeroGRO™ BBE Agar 03 MacConkey Biplate 13 AnaeroGRO™ BBE/PEA 03 Bovine Selective Strep Agar 13 AnaeroGRO™ Brucella Agar 03 Brucella Agar with 5% Sheep Blood, Hemin, AnaeroGRO™ Campylobacter and Vitamin K 13 Selective Agar 03 Brucella Broth with 15% Glycerol 13 AnaeroGRO™ CCFA 03 Brucella with H and K/LKV Biplate 14 AnaeroGRO™ Egg Yolk Agar, Modifi ed 03 Buffered Peptone Water 14 AnaeroGRO™ LKV Agar 03 Buffered Peptone Water with 1% AnaeroGRO™ PEA 03 Tween® 20 14 AnaeroGRO™ MultiPak A 04 Buffered NaCl Peptone EP, USP 14 AnaeroGRO™ MultiPak B 04 Butterfi eld’s Phosphate Buffer 14 AnaeroGRO™ Chopped Meat Broth 05 Campy Cefex Agar, Modifi ed 14 AnaeroGRO™ Chopped Meat Campy CVA Agar 14 Carbohydrate Broth 05 Campy FDA Agar 14 AnaeroGRO™ Chopped Meat Campy, Blood Free, Karmali Agar 14 Glucose Broth 05 Cetrimide Select Agar, USP 14 AnaeroGRO™ Thioglycollate with Hemin and CET/MAC/VJ Triplate 14 Vitamin K (H and K), without Indicator 05 CGB Agar for Cryptococcus 14 Anaerobic PEA 08 Chocolate Agar 15 Baird-Parker Agar 08 Chocolate/Martin Lewis with Barney Miller Medium 08 Lincomycin Biplate 15 BBE Agar 08 CompactDry™ SL 16 BBE Agar/PEA Agar 08 CompactDry™ LS 16 BBE/LKV Biplate 09 CompactDry™ TC 17 BCSA 09 CompactDry™ EC 17 BCYE Agar 09 CompactDry™ YMR 17 BCYE Selective Agar with CAV 09 CompactDry™ ETB 17 BCYE Selective Agar with CCVC 09 CompactDry™ YM 17 BCYE with DGVP 09 CompactDry™ XSA 17 BCYE Selective Agar with GVPC 09 Derm-Duet™ II DTM/RSM Biplate 18 BE Agar 09 Derm-Duet™ RSM/DTM Biplate 18 BE Agar with Azide 09 Dermatophyte Milk Agar 18 BE Agar with Azide/CNA Biplate 09 Dermatophyte Test Medium Agar 18 BG Agar 10 SabDex Agar with C and G/DTM Biplate 18 BG Agar with Novobiocin 10 Dilu-Lok™ Pre-fi lled Dilution Vials 19 Headquarters BG Bile Broth with Durham Tube 10 Chocolate/MTM Biplate 20 1430 West McCoy Lane BHI Agar 10 Chocolate Agar with Bacitracin 20 Santa Maria, CA 93455 BHI Agar with Blood 10 CIN/MacConkey with Sorbitol Biplate 20 800.266.2222 : phone [email protected] BHI Agar with Blood and Gentamicin 11 CIN Agar 20 www.HardyDiagnostics.com BHI Agar, with Blood, Chloramphenicol, CLED Agar 20 and Gentamicin 11 CNA Agar 20 Distribution Centers BHI Agar with Chloramphenicol and CNA Agar/EMB Agar Biplate 20 Santa Maria, California Cycloheximide 11 CNA Agar/MacConkey Agar Biplate 21 Olympia, Washington Salt Lake City, Utah BHI Agar with Vancomycin 11 Columbia Agar, USP 21 Phoenix, Arizona BHI Broth 11 Columbia Agar, 5% Sheep Blood 21 Dallas, Texas Springboro, Ohio BHI Broth with 5% Fildes Solution 11 Columbia Agar with 5% Sheep Blood/ Lake City, Florida BiGGY Agar 11 MacConkey Agar Biplate 21 Albany, New York Raleigh, North Carolina Blood Agar, 5% 12 Cooked Meat with Iron, H and K 21 Blood Agar, 5%/Chocolate Biplate 12 Corn Meal Agar with Tween® 80 21 Blood Agar 5%/EMB Biplate 12 CT-SMAC 21 The Quality Management Blood Agar, 5%/MacConkey Biplate 12 CTA 22 System at the Hardy Blood Agar, 5% with Ampicillin 12 D/E Neutralizing Broth 22 Diagnostics manufacturing facility is certifi ed to ISO Blood Agar, 8% 13 D/E Neutralizing Agar 22 13485. Blood Agar, 10% 13 DNase Agar with Toluidine Blue 22 Blood Agar, EH 13 Diamonds Medium, Modifi ed 22 Blood, Sheep, Defi brinated 13 Dichloran-Glycerol (DG-18) Agar 22 Bovine Blood Agar, 5% 13 EC Broth with Durham Tube 22 EC Broth with MUG iii22 Copyright © 2019 Hardy Diagnostics EE Broth Mossel, USP 23 Letheen Agar, Modifi ed with MSA with Oxacillin 38 Egg Yolk Agar, Modifi ed 23 Tween® 80, 1.5% 32 Mueller Hinton Agar 38 EMB Levine Agar 23 Letheen Agar/MacConkey Agar Biplate 33 Mueller Hinton Agar with Blood 39 EnteroScreen 4™ 23 Letheen Broth 33 Mueller Hinton Broth 39 EnviroTrans™ Swab Rinse Kit 24 Letheen Broth, Modifi ed 33 Mycobiotic Agar 39 FB Broth 24 LIA 33 Mycoplasma Agar 39 Fluid A, USP 24 Liver Infusion Agar Selective 33 Mycoplasma Broth 39 Fluid D, USP 24 LJ Gruft 33 MycoVue™ Slide Culture System 40 Fluid K, USP 24 LJ Medium 33 MYP Agar 40 Fraser Broth, Modifi ed 24 LJ with Ferric Ammonium Citrate, Slant 33 NaCl (Salt Tolerance) 6.5% Broth 40 FTM with Lecithin and Tween® 25 LJ with Pyruvate 33 Neomycin Anaerobic Blood Agar 40 FTM with Hemin and Vitamin K 25 LKV Agar 34 Neutralizing Buffer 40 GBS Detect™ 25 Loeffl er’s Slant 34 Nitrate Broth with Durham Tube 41 GN Broth 25 m EI Agar 34 Non-Nutrient Agar Plate 41 Group A Beta Strep Agar 25 m Endo LES Agar 34 Nutrient Agar 41 Haemophilus ID Quadplate 25 m FC Agar with 1% Rosolic Acid 34 Nutrient Agar with MUG 41 HardyCHROM™ BluEcoli™ Biplate 26 m HPC Agar 35 Nutrient Broth 41 HardyCHROM™ Candida 26 m PA Agar 35 OF Media 41 HardyCHROM™ ECC 26 m TEC Agar, Modifi ed 35 ONPG Rapid Test Broth 41 HardyCHROM™ ESBL 27 MacConkey Agar 35 Orange Serum Agar 41 HardyCHROM™ HUrBi™ 27 MacConkey Agar with Cefotaxime 35 Oxford Medium, Modifi ed 41 HardyCHROM™ Listeria 27 MacConkey Agar with Sorbitol 35 Page’s Saline 41 HardyCHROM™ MRSA 27 MacConkey/EMB Biplate 35 PALCAM 41 HardyCHROM™ O157 28 MacConkey Broth 36 PC Agar 41 HardyCHROM™ Sakazakii 28 Malt Extract Agar 36 Phenylalanine Agar Slant 42 HardyCHROM™ Salmonella 28 Malt Extract Agar with Lecithin and Tween 36 PEA with 5% Sheep Blood 42 HardyCHROM™ Salmonella/XLT-4 Malt Extract Agar with 0.01% Peptic Digest Agar with Fildes Agar Biplate 28 Chloramphenicol 36 and Nafcillin 42 HardyCHROM™ SS NoPRO 28 Martin Lewis with Lincomycin 36 Peptone Water 42 HardyCHROM™ Staph aureus 29 Mastitis Triplate 37 Peptone Salt 42 HardyCHROM™ UTI 29 McFarland Standard 37 Phenol Red Broth with Durham Tube 42 HardyCHROM™ Vibrio 30 Middlebrook 7H10 Agar 37 Phosphate Buffered Saline (PBS), pH 6.8 42 HE Agar 30 Middlebrook 7H11/7H11 Selective Phosphate Buffered Saline (0.01M) with HE Agar/SS Agar Biplate 31 Agar Biplate 37 0.02% Tween® 80 42 HTM Agar 31 Middlebrook 7H11 Agar 37 Phosphate Buffered Saline, pH 7.5 43 Inhibitory Mold Agar 31 Middlebrook 7H11 Selective 37 Phosphate Buffer with Magnesium Inhibitory Mold Agar with Gentamicin 31 Middlebrook 7H11, Thin Pour 37 Chloride 43 Iodine-Iodide Solution 31 Middlebrook 7H9 Broth 37 Phosphate Buffered Saline with 0.05% KF Streptococcus Agar 31 Middlebrook 7H9 Broth with Tween® 80 37 Tween 20 43 KIA Slant 31 MIL Medium 37 PLET Agar 43 Lactobacilli MRS Agar 31 MIO Medium 38 Potato Dextrose Agar 43 Lactobacilli MRS Broth 32 Moeller’s Decarboxylase 38 Potato Dextrose Agar with Lactose Broth 32 Motility Test Medium with TTC 38 Chlortetracycline 43 Lauryl Tryptose Broth with Durham Tube 32 Motility Test Medium without TTC 38 Potato Dextrose Agar with Leeds Medium 32 MRSA Screen Plate 38 Chloramphenicol 43 Letheen Agar 32 MR-VP Broth 38 Potato Dextrose Agar with TA 43 Letheeniv Agar, Modifi ed 32 MSA Agar 38 Potato Flake Agar 43 TCBS Agar 51 Urea Medium, Rapid 54 Tergitol 7 Agar with TTC 51 UVM Modifi ed Listeria Broth 54 Tetrathionate Broth 51 V9 Agar 54 Tetrathionate Broth with Brilliant Green 51 Vogel and Johnson Agar 54 Thayer Martin Agar 51 VRBA 54 Thayer Martin Agar, Modifi ed 52 VRBGA 54 Thioglycollate with H and K 52 Wallenstein Media 54 Thioglycollate without Indicator 52 Water with Tween® 80 54 Thioglycollate without Indicator, with XLD Agar 54 Supplements 52 XLT4 Agar 54 Thioglycollate with Indicator 52 XLT4 Agar/Brilliant Green Agar with Todd Hewitt Broth 52 Sulfadiazine Biplate 54 Todd Hewitt Broth with SXT 52 Tryptic Soy Broth (TSB), USP, Potato Flake Selective Agar 44 Trehalose Broth, Rapid 52 ReadyRack™ 56 PPLO Selective Agar 44 Tryptic Soy Agar 52 Fluid Thioglycollate Medium (FTM), USP, Pseudomonas Agar F 44 TSA, SterEM™, Irradiated, USP 52 ReadyRack™ 56 Pseudomonas Isolation Agar 44 TSA with Cycloheximide 52 R10 Broth 44 TSA with Lecithin and Tween® 80 52 Rapid Ornithine 44 TSA Blood/CLED/MAC 53 Regan-Lowe Agar 45 TSA with Lecithin and Tween® 80, USP 53 Regan-Lowe Semisolid 45 TSB, USP, Bottles 53 Rose Agar 45 TSB, Double Strength, USP 53 Rose/MacConkey Biplate 45 TSB Tubes 53 Rose Bengal Agar with Chloramphenicol 45 TSB with 6.5% Sodium Chloride 54 RTF Modifi ed Casman Agar 46 TSB with Lecithin and Tween® 20, USP 54 RV Broth, USP 46 TSB with Lecithin and Tween® 80 54 SabDex Agar 46 TSI Agar 54 SabDex Agar, Emmons 46 Tryptone Broth, 2% 54 SabDex Agar with C and G/DTM Biplate 46 Tryptone Glucose Extract Agar 54 SabDex Agar with Chloramphenicol 46 Urea (Christensen’s Urea) Agar Slant 54 SabDex Agar with Chloramphenicol and Tetracycline 46 SabDex Agar with Gentamicin and Chloramphenicol 46 SabDex Agar with Lecithin and Tween® 80 46 SabDex Agar with Lecithin and Tween® 80, SterEM™, Irradiated 46 SabDex Broth 47 SabHI Agar 47 SabHI Agar with Blood, Chloramphenicol, and Cycloheximide 47 Saline, 0.45% 47 Saline, 0.85% 47 Saline, 0.9% with 0.5% Tween® 80 48 Selective Beta Strep Agar, Group A 48 Selective Strep Agar (COBA Medium) 48 Selenite Cystine Broth 48 SIM Medium 48 Simmons Citrate Agar 49 Skim Milk Agar 49 SP4 Agar 49 SP4 Broth 49 SS Agar 49 Standard Methods Agar 49 Sterile Deionized Water 49 Sterile Water 49 Strep B Carrot Broth™ One-Step 50 TAT Broth 51 TB Base Digestant 51 v SELECTION Since 1980, Hardy Diagnostics has grown to manufacture an extensive selection of prepared media products which are PREPARED packaged in plates, tubes, bottles, bags, and flasks.
Load more

Recommended publications
  • Meningitis Manual Text
    Laboratory Methods for the Diagnosis of MENINGITIS Caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae Centers for Disease Control and Prevention August, 1998 Laboratory Methods for the Diagnosis of Meningitis Caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae Table of Contents Introduction………………………………………………………………………………… 1 Acknowledgments ……………………………………………………………………….. 2 I. Epidemiology of Meningitis Caused by Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae,…………………………………………… 3 II. General Considerations ......................................................................................................... 5 A. Record Keeping ................................................................................................................... 5 III. Collection and Transport of Clinical Specimens ................................................................... 6 A. Collection of Cerebrospinal Fluid (CSF)............................................................................... 6 A1. Lumbar Puncture ................................................................................................... 6 B. Collection of Blood .............................................................................................................. 7 B1. Precautions ............................................................................................................ 7 B2. Sensitivity of Blood Cultures ................................................................................
    [Show full text]
  • Enterobacteriaceae Family (CRE), Is of Utmost Importance for the Enterobacteriaceae Management of Infected Or Colonized Patients
    2013 iMedPub Journals THE INTERNATIONAL ARABIC JOURNAL Vol. 3 No. 3:5 Our Site: http://www.imedpub.com/ OF ANTIMICROBIAL AGENTS doi: 10.3823/737 Evaluation of Rula Al-Dawodi1,3, Rawan Liddawi1,3, Raed Ghneim1,3, Randa Kattan1,3, Issa Siryani1,3, Afaf Abu-Diab1, Riyad Meropenem, Ghneim1, Madeleine Zoughbi1,3, Abed-El-Razeq Issa1,3, Randa Al Qass1,3, Sultan Turkuman1, Hiyam Marzouqa1, and Imipenem and Musa Hindiyeh1,2,3* Ertapenem 1 Caritas Baby Hospital, 3 Palestinian Forum for Medical * Correspondence: Bethlehem, Palestine; Research (PFMR), Ramallah, Impregnated 2 Bethlehem University, Palestine Bethlehem, Palestine; [email protected] MacConkey * Musa Y Hindiyeh, Caritas Baby Hospital Bethlehem Agar Plates for Palestine. the Detection of Carbapenem Abstract Resistant Background: Rapid detection of carbapenem resistant bacteria, in particular, members of the Enterobacteriaceae family (CRE), is of utmost importance for the Enterobacteriaceae management of infected or colonized patients. Methods: Three carbapenems; meropenem, imipenem and ertapenem, with two different concentrations (0.5 mg/ml and 1.0 mg/ml), were impregnated in Mac- Conkey agar. The carbapenem impregnated MacConkey agar plates; ([Mac-Mem], [Mac-Imp] and [Mac-Ert]), were then evaluated for the detection of carbapenem resistant Gram-negative bacteria in particular the blaKPC producing Enterobacteria- ceae. The Limit of Detection (LOD) of the plates was determined in triplicate after serial logarithmic dilution of the bacterial strains in saline. This was followed by inoculating the plates and counting the colonies that grew after 24 hours of incu- bation. The specificity and the shelf-life of the plates were determined by testing the plates with six Extended Spectrum β-lactamases (ESBL) producing members of the Enterobacteriaceae family and one genus with the blaAmpC phenotype.
    [Show full text]
  • Chocolate Agar Plate MP103 Intended Use for Isolation of Neisseria Gonorrhoeae from Chronic and Acute Gonococcal Infections
    Chocolate Agar Plate MP103 Intended use For isolation of Neisseria gonorrhoeae from chronic and acute gonococcal infections. Composition** Ingredients Gms / Litre Proteose peptone 20.000 Dextrose 0.500 Sodium chloride 5.000 Disodium phosphate 5.000 Agar 15.000 After sterilization Sterile Lysed blood (at 80°C) 50.000 Vitamino Growth Supplement (FD025) 2 vials Final pH ( at 25°C) 7.3±0.2 **Formula adjusted, standardized to suit performance parameters Directions Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate. Principle And Interpretation Neisseria gonorrhoeae is a gram-negative bacteria and the causative agent of gonorrhea, however it is also occasionally found in the throat. The cultivation medium for gonococci should ideally be a rich nutrients base with blood, either partially lysed or completely lysed. The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoeae. Chocolate Agar Base, with the addition of supplements, gives excellent growth of the gonococcus without overgrowth by contaminating organisms. G.C. Agar (M434) can also be used in place of Chocolate Agar Base, which gives slightly better results than Chocolate Agar (4). The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoea. Interest in the cultural procedure for the diagnosis of gonococcal infection was stimulated by Ruys and Jens (9), Mcleod and co-workers (8), Thompson (7), Leahy and Carpenter (1), Carpenter, Leahy and Wilson (2) and Carpenter (10), who clearly demonstrated the superiority of this method over the microscopic technique.
    [Show full text]
  • Dehydrated Culture Media
    Dehydrated Culture Media Manufactured by Dehydrated Culture Media Table of Contents 4 CRITERION™ Products 12 Supplements and Antibiotics 13 CRITERION™ Agarose for Gel Electrophoresis Dehydrated Culture Media ™ TM Hardy Diagnostics’ dehydrated culture media, CRITERION , is formulated to meet or exceed the highest quality standards. DEHYDRATED CULTURE MEDIA Choose from 250 standard formulas or request custom blending to your specifications. The innovative packaging designs and overall reliability makeCRITERION ™ the logical choice for culture media in your laboratory. FEATURES & BENEFITS Hand Grip Convenient hand-grip design features finger indentations to allow for easy and safe handling of the bottle. Induction Seal Gray Jar Pull-off induction seal prevents moisture from clumping the media, keeping it fresh and dry. Opaque gray jar diminishes Wide Mouth Opening light penetration, • Allows for easy access to use a scoop when prolonging superior measuring the powder. performance and • Prevents inhalation hazards and reduces shelf life. hazardous dust formations. • No more shaking the bottle to dispense the media. Desiccant Pack A silica gel pack is included in each bottle to prevent clumping. Reusable Seal A built-in cushion seal inside the lid prevents moisture from entering the previously opened container. 1 UNPARALLELED PERFORMANCE Every formulation and lot is thoroughly tested for optimal growth characteristics. WIDE MOUTH OPENING Scooping media from the wide mouth bottle, instead of pouring and shaking, reduces dangerous dust formation CONVENIENT SIZES Packaged in four standard sizes to fit your needs: • 2 liter Mylar® bag (pre-measured to make 2 liters of culture media) • 500gm bottle • 2kg buckets with locking screw top lid • 10kg buckets with locking screw top lid STACKABLE Bottles and buckets have a nesting design and are stackable for efficient and economical storage.
    [Show full text]
  • Dehydrated Culture Media Description Packaging Ref
    Product Catalogue 2016 © Liofilchem® s.r.l. Clinical and Industrial Microbiology Est. 1983 Dehydrated Culture Media Description Packaging Ref. A1 Medium APHA 500 g 610105 Basal liquid medium for fecal coliforms detection in water and food. 100 g 620105 TRITON X 100 supplement 5x5 mL 80046 Acetamide Agar 500 g 610312 Medium for differentiation of nonfermentative, Gram-negative bacteria, especially Pseudomonas aeruginosa, on the basis of acetamide utilization. Acetamide Broth 500 g 610313 Broth for the differentiation of nonfermentative, Gram-negative bacteria, especially Pseudomonas aeruginosa, on the basis of acetamide utilization. Aeromonas Agar Base 500 g 610048 Basal medium for selective isolation of Aeromonas spp. 100 g 620048 Ampicillin supplement 10 vials 81001 Alkaline Peptone Water APHA 500 g 610098 Liquid enrichment medium for Vibrio spp. isolation. 100 g 620098 Amies Transport Medium (with charcoal) 500 g 610152 Semi-solid medium for transport of clinical, environmental specimens and of 100 g 620152 microorganisms. 5 kg 6101525 Amies Transport Medium (w/o charcoal) 500 g 610191 Semi-solid medium for transport of clinical, environmental specimens and of 100 g 620191 microorganisms. 5 kg 6101915 Anaerobic Agar (Brewer) 500 g 610320 Medium for cultivating anaerobic microorganisms. Andrade Lactose Peptone Water 500 g 610118 Liquid medium for coliforms detection with andrade's indicator. 100 g 620118 Andrade Peptone Water 500 g 610119 Liquid enrichment medium with andrade's indicator. 100 g 620119 Antibiotic Agar No.1 E.P. 500 g 610314 Surface medium for the antibiotic assay by Agar-diffusion method. Antibiotic Broth No.3 U.S.P. 500 g 610316 Broth for turbidimetric assay of antibiotics.
    [Show full text]
  • Bile Esculin Agar
    BILE ESCULIN AGAR INTENDED USE Remel Bile Esculin Agar is a solid medium recommended for use in qualitative procedures for the presumptive identification of group D streptococci and enterococci. SUMMARY AND EXPLANATION Rochaix first demonstrated the value of esculin hydrolysis for identification of enterococci.1 Meyer et al. found that 61 of 62 strains of enterococci hydrolyzed esculin in a medium containing bile.2 Swan determined that positive results obtained on esculin agar containing 40% bile correlated well with serologically confirmed group D streptococci.3 Using Swan’s formula, Facklam and Moody tested over 700 strains of streptococci and enterococci representing all known serological groups and found all strains to be bile-resistant and esculin-positive. PRINCIPLE Group D streptococci and enterococci hydrolyze esculin in the presence of bile to form esculetin and dextrose. Esculetin reacts with ferric ions supplied by ferric ammonium citrate to form brown-black colonies on Bile Esculin Agar. Oxgall in a concentration of 4% (equivalent to 40% bile) inhibits most strains of streptococci and enterococci other than group D. REAGENTS (CLASSICAL FORMULA)* Oxgall (40% Bile) ............................................................ 40.0 g Esculin...............................................................................1.0 g Gelatin Peptone ................................................................ 5.0 g Ferric Ammonium Citrate...................................................0.5 g Beef Extract....................................................................... 3.0 g Agar.................................................................................15.0 g Demineralized Water...................................................1000.0 ml pH 6.8 ± 0.2 @ 25°C *Adjusted as required to meet performance standards. PRECAUTIONS This product is For Laboratory Use only. It is not intended for use in the diagnosis of disease or other conditions. PREPARATION OF DEHYDRATED CULTURE MEDIUM 1. Suspend 64 g of medium in 1000 ml of demineralized water.
    [Show full text]
  • Multicellular Oxidant Defense in Unicellular Organisms MUCHOU MA and JOHN W
    Proc. Natl. Acad. Sci. USA Vol. 89, pp. 7924-7928, September 1992 Microbiology Multicellular oxidant defense in unicellular organisms MUCHOU MA AND JOHN W. EATON* Division of Experimental Pathology, Department of Pathology and Laboratory Medicine, Albany Medical College, A-81, 47 New Scotland Avenue, Albany, NY 12208 Communicated by David W. Talmage, May 8, 1992 ABSTRACT Although catalase is thought to be a major MATERIALS AND METHODS defense against hydrogen peroxide (H202), the catalase activity Reagents. Brain heart infusion broth, Todd-Hewitt broth, within individual Escherichia coil fails to protect against ex- Lennox L agar (LB agar), and Bactoagar were obtained from ogenous H202. Contrary to earlier reports, we find that dilute GIBCO/BRL. The bicinchoninic acid protein microassay suspensions, of wild-type and catalase-deficient E. colt are was from Pierce. All other enzymes and chemicals were identical in their sensitivity to H202, perhaps because even purchased from Sigma. wild-type, catalase-positive E. colU cannot maintain an inter- Bacterial Strains and Culture Conditions. A catalase- nal/externail concentration gradient of this highly diffusible deficient mutant strain of E. coli K-12 [UM1, hereafter, oxidant. However, concentrated suspensions or colonies of cat(-)] and its parent wild-type [CSH7, hereafter cat(+)] (17) catalase-positive E. colt do preferentially survive H202 chal- were provided by P. C. Loewen (University ofManitoba). E. lenge and can even cross-protect adjacent catalase-deficient coli were grown statically in brain heart infusion broth or M9 organisms. Furthermore, high-density catalase-positive-but minimal salts medium supplemented with 10 mM glucose not catalase-negative-E. colt can survive and multiply in the (M9/glucose) (25) at 370C in room air overnight (18-20 hr) to presence of competitive, peroxide-generating streptococci.
    [Show full text]
  • Evaluating Historical Paradigms of Sterility in Perinatal Microbiology and Ramifications Orf Pregnancy Outcomes
    Wayne State University Wayne State University Dissertations January 2020 Evaluating Historical Paradigms Of Sterility In Perinatal Microbiology And Ramifications orF Pregnancy Outcomes Jonathan Greenberg Wayne State University Follow this and additional works at: https://digitalcommons.wayne.edu/oa_dissertations Part of the Microbiology Commons, and the Obstetrics and Gynecology Commons Recommended Citation Greenberg, Jonathan, "Evaluating Historical Paradigms Of Sterility In Perinatal Microbiology And Ramifications orF Pregnancy Outcomes" (2020). Wayne State University Dissertations. 2468. https://digitalcommons.wayne.edu/oa_dissertations/2468 This Open Access Dissertation is brought to you for free and open access by DigitalCommons@WayneState. It has been accepted for inclusion in Wayne State University Dissertations by an authorized administrator of DigitalCommons@WayneState. EVALUATING HISTORICAL PARADIGMS OF STERILITY IN PERINATAL MICROBIOLOGY AND RAMIFICATIONS FOR PREGNANCY OUTCOMES by JONATHAN MURRAY GREENBERG DISSERTATION Submitted to the Graduate School of Wayne State University, Detroit, Michigan in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY 2020 MAJOR: BIOCHEMISTRY, MICROBIOLOGY, & IMMUNOLOGY Approved by: Advisor Date DEDICATION This dissertation is dedicated to my parents and sister, who have all been immeasurably supportive over my graduate career and more importantly my entire life. I could not have done this without you. ii ACKNOWLEDGMENTS There is no way for me to express fully my sincere gratitude to all the individuals who have had an impact on my life and throughout my time here at Wayne State. None of it would have been possible without Dr. Kevin Theis. Rotating in his lab was one of the best decisions of my life and quite literally got me to where I am.
    [Show full text]
  • Macconkey Agar, CS, Product Information
    MacCONKEY AGAR, CS (7391) Intended Use MacConkey Agar, CS is used for the isolation and differentiation of Gram-negative enteric bacilli from specimens containing swarming strains of Proteus spp. in a laboratory setting. MacConkey Agar, CS is not intended for use in the diagnosis of disease or other conditions in humans Product Summary and Explanation MacConkey Agar is based on the bile salt-neutral red-lactose agar of MacConkey.1 The original MacConkey medium was used to differentiate strains of Salmonella typhosa from members of the coliform group. Formula modifications improved growth of Shigella and Salmonella strains. These modifications include the addition of 0.5% sodium chloride, decreased agar content, altered bile salts, and neutral red concentrations. The formula modifications improved differential reactions between enteric pathogens and coliforms. MacConkey Agar, CS (“Controlled Swarming”) contains carefully selected raw materials to reduce swarming of Proteus spp., which could cause difficulty in isolating and enumerating other Gram-negative bacilli. Principles of the Procedure Enzymatic Digest of Gelatin, Enzymatic Digest of Casein, and Enzymatic Digest of Animal Tissue are the nitrogen and vitamin sources in MacConkey Agar, CS. Lactose is the fermentable carbohydrate. During Lactose fermentation a local pH drop around the colony causes a color change in the pH indicator, Neutral Red, and bile precipitation. Bile Salts and Crystal Violet are the selective agents, inhibiting Gram-positive cocci and allowing Gram-negative organisms to grow. Sodium Chloride maintains the osmotic environment. Agar is the solidifying agent. Formula / Liter Enzymatic Digest of Gelatin .................................................... 17 g Enzymatic Digest of Casein ................................................... 1.5 g Enzymatic Digest of Animal Tissue.......................................
    [Show full text]
  • Francisella Tularensis 6/06 Tularemia Is a Commonly Acquired Laboratory Colony Morphology Infection; All Work on Suspect F
    Francisella tularensis 6/06 Tularemia is a commonly acquired laboratory Colony Morphology infection; all work on suspect F. tularensis cultures .Aerobic, fastidious, requires cysteine for growth should be performed at minimum under BSL2 .Grows poorly on Blood Agar (BA) conditions with BSL3 practices. .Chocolate Agar (CA): tiny, grey-white, opaque A colonies, 1-2 mm ≥48hr B .Cysteine Heart Agar (CHA): greenish-blue colonies, 2-4 mm ≥48h .Colonies are butyrous and smooth Gram Stain .Tiny, 0.2–0.7 μm pleomorphic, poorly stained gram-negative coccobacilli .Mostly single cells Growth on BA (A) 48 h, (B) 72 h Biochemical/Test Reactions .Oxidase: Negative A B .Catalase: Weak positive .Urease: Negative Additional Information .Can be misidentified as: Haemophilus influenzae, Actinobacillus spp. by automated ID systems .Infective Dose: 10 colony forming units Biosafety Level 3 agent (once Francisella tularensis is . Growth on CA (A) 48 h, (B) 72 h suspected, work should only be done in a certified Class II Biosafety Cabinet) .Transmission: Inhalation, insect bite, contact with tissues or bodily fluids of infected animals .Contagious: No Acceptable Specimen Types .Tissue biopsy .Whole blood: 5-10 ml blood in EDTA, and/or Inoculated blood culture bottle Swab of lesion in transport media . Gram stain Sentinel Laboratory Rule-Out of Francisella tularensis Oxidase Little to no growth on BA >48 h Small, grey-white opaque colonies on CA after ≥48 h at 35/37ºC Positive Weak Negative Positive Catalase Tiny, pleomorphic, faintly stained, gram-negative coccobacilli (red, round, and random) Perform all additional work in a certified Class II Positive Biosafety Cabinet Weak Negative Positive *Oxidase: Negative Urease *Catalase: Weak positive *Urease: Negative *Oxidase, Catalase, and Urease: Appearances of test results are not agent-specific.
    [Show full text]
  • Genital Brucella Suis Biovar 2 Infection of Wild Boar (Sus Scrofa) Hunted in Tuscany (Italy)
    microorganisms Article Genital Brucella suis Biovar 2 Infection of Wild Boar (Sus scrofa) Hunted in Tuscany (Italy) Giovanni Cilia * , Filippo Fratini , Barbara Turchi, Marta Angelini, Domenico Cerri and Fabrizio Bertelloni Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy; fi[email protected] (F.F.); [email protected] (B.T.); [email protected] (M.A.); [email protected] (D.C.); [email protected] (F.B.) * Correspondence: [email protected] Abstract: Brucellosis is a zoonosis caused by different Brucella species. Wild boar (Sus scrofa) could be infected by some species and represents an important reservoir, especially for B. suis biovar 2. This study aimed to investigate the prevalence of Brucella spp. by serological and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. From 287 animals, sera, lymph nodes, livers, spleens, and reproductive system organs were collected. Within sera, 16 (5.74%) were positive to both rose bengal test (RBT) and complement fixation test (CFT), with titres ranging from 1:4 to 1:16 (corresponding to 20 and 80 ICFTU/mL, respectively). Brucella spp. DNA was detected in four lymph nodes (1.40%), five epididymides (1.74%), and one fetus pool (2.22%). All positive PCR samples belonged to Brucella suis biovar 2. The results of this investigation confirmed that wild boar represents a host for B. suis biovar. 2 and plays an important role in the epidemiology of brucellosis in central Italy. Additionally, epididymis localization confirms the possible venereal transmission. Citation: Cilia, G.; Fratini, F.; Turchi, B.; Angelini, M.; Cerri, D.; Bertelloni, Keywords: Brucella suis biovar 2; wild boar; surveillance; epidemiology; reproductive system F.
    [Show full text]
  • Pocket Guide to Clinical Microbiology
    4TH EDITION Pocket Guide to Clinical Microbiology Christopher D. Doern 4TH EDITION POCKET GUIDE TO Clinical Microbiology 4TH EDITION POCKET GUIDE TO Clinical Microbiology Christopher D. Doern, PhD, D(ABMM) Assistant Professor, Pathology Director of Clinical Microbiology Virginia Commonwealth University Health System Medical College of Virginia Campus Washington, DC Copyright © 2018 Amer i can Society for Microbiology. All rights re served. No part of this publi ca tion may be re pro duced or trans mit ted in whole or in part or re used in any form or by any means, elec tronic or me chan i cal, in clud ing pho to copy ing and re cord ing, or by any in for ma tion stor age and re trieval sys tem, with out per mis sion in writ ing from the pub lish er. Disclaimer: To the best of the pub lish er’s knowl edge, this pub li ca tion pro­ vi des in for ma tion con cern ing the sub ject mat ter cov ered that is ac cu rate as of the date of pub li ca tion. The pub lisher is not pro vid ing le gal, med i cal, or other pro fes sional ser vices. Any ref er ence herein to any spe cific com mer cial prod ucts, pro ce dures, or ser vices by trade name, trade mark, man u fac turer, or oth er wise does not con sti tute or im ply en dorse ment, rec om men da tion, or fa vored sta tus by the Ameri can Society for Microbiology (ASM).
    [Show full text]