B3GAT1 Antibody Cat

B3GAT1 Antibody Cat. No.: 64-189 B3GAT1 Antibody Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti- rabbit secondary antibodies were used for the analysis. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human This B3GAT1 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 21-48 amino acids from the N-terminal region of human B3GAT1. TESTED APPLICATIONS: Flow, WB For WB starting dilution is: 1:1000 APPLICATIONS: For FACS starting dilution is: 1:10~50 PREDICTED MOLECULAR 38 kDa WEIGHT: September 26, 2021 1 https://www.prosci-inc.com/b3gat1-antibody-64-189.html Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: B3GAT1 Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1,3- ALTERNATE NAMES: glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P, B3GAT1, GLCATP ACCESSION NO.: Q9P2W7 PROTEIN GI NO.: 205830910 GENE ID: 27087 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars BACKGROUND: and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). REFERENCES: 1) Petrovas, C., et al. J. Immunol. 183(2):1120-1132(2009) 2) Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009) 3) Chong, L.K., et al. Eur. J. Immunol. 38(4):995-1000(2008) 4) Casado, J.G., et al. Tumour Biol. 29(5):304-310(2008) September 26, 2021 2 https://www.prosci-inc.com/b3gat1-antibody-64-189.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/b3gat1-antibody-64-189.html.

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List of Genes Used in Cell Type Enrichment Analysis
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Markers of T Cell Senescence in Humans
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Human Β‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody Monoclonal Mouse Igg2a Clone # 1002707 Catalog Number: MAB8560
Human β‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody Monoclonal Mouse IgG2A Clone # 1002707 Catalog Number: MAB8560 DESCRIPTION Species Reactivity Human Specificity Detects human β-1,3-Glucuronyltransferase 1/B3GAT1 in direct ELISAs. Source Monoclonal Mouse IgG2A Clone # 1002707 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Chinese Hamster Ovary cell line, CHO-derived human β‑1,3‑Glucuronyltransferase 1/B3GAT1 His25-Ile334 Accession # Q9P2W7 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS. APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Immunohistochemistry 5-25 µg/mL See Below DATA Immunohistochemistry β-1,3-Glucuronyltransferase 1/B3GAT1 in Human Brain. β-1,3-Glucuronyltransferase 1/B3GAT1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human β-1,3- Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB8560) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
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B3GAT1 Monoclonal Antibody, Clone NK-1
B3GAT1 monoclonal antibody, Gene Alias: CD57, GLCATP, GlcAT-P, GlcUAT-P, clone NK-1 (PE) HNK-1, HNK1, LEU7, NK-1 Catalog Number: MAB6074 Gene Summary: The protein encoded by this gene is a member of the glucuronyltransferase gene family. These Regulatory Status: For research use only (RUO) enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric Product Description: Mouse monoclonal antibody linkages. This gene product functions as the key enzyme raised against native B3GAT1. in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural Clone Name: NK-1 killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. Immunogen: Native purified from human peripheral [provided by RefSeq] blood mononuclear cells. References: Host: Mouse 1. CD57+ T lymphocytes are derived from CD57- precursors by differentiation occurring in late immune Reactivity: Human responses. d'Angeac AD, Monier S, Pilling D, Travaglio-Encinoza A, Reme T, Salmon M. Eur J Applications: Flow Cyt, IHC-Fr Immunol. 1994 Jul;24(7):1503-11. (See our web site product page for detailed applications 2. Characterization of HNK-1+ (Leu-7) human information) lymphocytes. I. Two distinct phenotypes of human NK Protocols: See our web site at cells with different cytotoxic capability. Abo T, Cooper http://www.abnova.com/support/protocols.asp or product MD, Balch CM. J Immunol. 1982 Oct;129(4):1752-7. page for detailed protocols 3. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). Abo T, Specificity: Specificity human CD57 Balch CM.
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