Patent Applications in the Area of Fermentation

Patent# Subject Assignee Author Date Status•

W09703185 Production of metabolites in Bacillus cells mutated Novo Nordisk AS Diderichsen B, Fleming AB, 01/30/97 A to prevent sporulation. Useful for the production of (Denmark) Jorgensen P. Priest JG, Industrial or medical enzymes. Tangney M, Jorgensen PLLWO 9705468 Describes the preparation of antigens from infectious and Mendes Pereira R Mendes PR, Mendes 02/13/97 A parasitic microorganisms. Useful for the diagnosis, therapy, Pereira R and prophylaxis of diseases such as Chagas' disease. JP9047292 Preparation of pyruvic acid and its salts, with improved Toray Industries Toray Industries 02/1 8/97 A fermentation efficiency yield and purity in supernatant. (Ehime, Japan) This is achieved by stepwise addition of nitrogen source during pyruvic acid fermentation by microorganisms. W09707231 Improved production of amphotericln B by microbial Rutgers University Kientzler DJ, 02/27/97 A fermentation-comprises selective inhibition of (New Brunswick, NJ) Schaffner CP amphotericln A production by addition of protein synthesis Inhibitor, e.g., streptomycin, to the nutrient medium. JP9065873 Describes the culture of yeast according to a mathematical Oriental Yeast Co. Oriental Yeast Co. 03/11 /97 A model. Produces required.quality with high precision. US5622845 Fermentatlve production of norleucine using Escherichia UpJohn Company Brunner DP, Garlick RL, 04/22/97 A coli in medium with low amino acid concentration. (Uppsala, Sweden) Harbour GC, Kirschner RJ , Pinner JF GB2305936 Describes a bacterial culture vessel able to hold Eliott TJ , Lewis JW Ell iott T J, Lewis JW 04/23/97 A encapsulated culture supplements. EP770676A Heat treatment of fermentation broth obtained by culturing Ajinomoto KK, Ohmori K, Tanabe T 05/02/97 A Escherichia coli tor increasing subsequent rate of permeation Ajinomoto Co. through membrane to separate cells. (Tokyo) W09716558 Method for positive selection of transformed Gram- E.1. Du Pont Bramucci MG, 05/09/97 A positive bacteria, especially Bacillus. Useful for cloning De Nemours & Co. Nagarajan V heterologous genes encoding, for example, industrial (Wilmington, DE) enzymes, lmmunoglobulin(s). W09718298 Long-term culture of hematopoietic stem cells or committed University of McGlave PB, Miller JS, 05/22/97 A progenitor(s) in the presence of stromal conditioned medium Minnesota Verfaillie CM and cytokine(s). Stems cells retain ability to differentiate. (Minneapolis, MN) JP9135696 Microbial preparation of arachidonic acid. Describes culture Suntory 05/27/97 A of Mortierella in a medium containing, e.g., fatty acid. (Osaka, Japan) W09719187 Describes a method for producing clavulanic acid, an Antibioticos SA Collados de la Vieja AJ, 05/29/97 A inhibitor of beta-lactamase(s) useful as antibiotic, by (Milan, Italy) Lopez Nieto MJ, Moreno submerged, continuous, or semicontinuous aerobic Valle MA, Rodriguez Otero C, fermentation of Streptomyces clavuligerus or its mutants. Vrtaller Alba A EP776978A Preparation of 4'-deoxy-O-mycaminosyltylonolide by Pfizer (New York) Lam LH 06/04/97 A aerobic fermentation of repromicin with Streptomyces fradiae. EP776975A Recombinant protein production using yeast containing Hoechst AG Aretz W, Badziong W, 06/04/97 A alcohol dehydrogenase II promoter in fed-batch process (Frankfurt) Habermann P, Moller J, with constant glucose supply. MoellerJ W09720834 Production of lovastatin from culture broth-has high purity Antibiotic Co. Dimitrova AS, Dimov DI , 06/ 12/ 97 A and stability, prepared using antioxidant, chlorine-containing Grozdanov GA, Petkov NG, solvents, etc. Todorovad DT W09721347 Cell culture apparatus formed of gas-permeable, silicon Avecor Cardio- Codner M 06/ 19/97 A membranes-sealed at edges to form bag-like vessel with vascular Inc. Interior chambers for containing cell culture media. (Bellshill, UK)

DE19548954 Preparation of culture solutions containing high molecular Hans-Knoell lnstitut Guenther E, Liebisch W, 07/03/97 A weight hyaluronic acids-by fermentation of Streptococcus fur Naturstoff- Mueller P, Ozegowski J, equi ssp. zooepidemicus V2541 , at constant pH and Forschung Veith G temperature, with maintenance of glucose supply. (Jena, Austria)

Source: Derwent lnfomiation, Alexandria, VA 'The patents in the table are pending. The status of each application is slightly different from country to country. For further details, contact De!Went lnfonnation, 1725 Duke St., Suite 250, Alexandria, VA 22314. Tel: 1 (800) DERWENT ([email protected]).

NATURE BIOTECHNOLOGY VOLUME 15 SEPTEMBER 1997 913